Cosmetic topical composition for improving capillary density containing oily extract of at least one moringa arabica seed as active ingredient, method for preparing composition and method for cosmetic treatment of hair

Abstract

The present invention relates to the field of cosmetics and, more specifically, to an oily extract of Moringa oleifera seeds as an active ingredient for improving capillary density. The invention also relates to a method for preparing said composition and to the cosmetic treatment of hair (hair).

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and more specifically to the field of active ingredients included in the formulation of skin care topical compositions. The present invention more specifically relates to an oily extract of Moringa peregrina seeds as an active ingredient for improving capillary density. The present invention also relates to a method for preparing said composition and to the cosmetic treatment of hair (hair). Background Art

[0002] It has long been known to those skilled in the art that natural hair loss in humans generally reflects a balance between hair follicles in alternating growth phases (anagen) and shedding phases (telogen). The average ratio of the number of follicles in the anagen phase to the number of follicles in the telogen phase is about 9 (90 / 10). Under normal physiological conditions, it is estimated that hundreds of hairs are naturally lost every day. It is also known that certain factors such as hormonal imbalances, physiological stress, and malnutrition can exacerbate this hair loss phenomenon. In certain scalp skin diseases with inflammatory features, such as, for example, psoriasis or seborrheic dermatitis, hair loss may be greatly exacerbated. If we want to respond effectively to the problem of hair loss, it is important to stop the shedding, but you must also do everything you can to stimulate the growth of new hair.

[0003] Furthermore, the risks of controversial ingredients in cosmetics have been widely criticized. Chemical treatments are increasingly being abandoned in favor of natural treatments.

[0004] In view of the above limitations, the applicant surprisingly found that a composition comprising as active ingredient an oil extracted from Moringa arabica seeds enables it to substantially improve capillary density. The oil may be virgin oil or winterized oil.

[0005] Virgin oil is obtained solely by physical methods, such as crushing or pressing the seeds, without the use of heat or chemical treatments.

[0006] Winterization of oil is the process of separating lipids and other desirable oil compounds from waxes using solvents and low temperatures. Fractional crystallization is used to separate different components using differences in their melting points, solubility, and volatility.

[0007] Applicants have found that the composition is particularly effective for hair regrowth and / or slowing hair loss. It appears that the natural composition itself provides improved capillary density without the need for added chemical ingredients.

[0008] The Moringaceae is a monogeneric family (only one genus, Moringa Adans), an element of the Saharo-Sindian flora, which, according to the authors, consists of between 12 and 14 species, distributed from eastern Africa to Asia. The genus is conventionally divided into three parts, which, however, have not been confirmed as monophyletic by phylogenetic analysis. Said analysis has shown, to some extent, a branch centered on certain morphological features: pachycauls ("bottle trees"); "tuberous trees" and those that are neither bottle trees nor tuberous trees ("slender trees"). The species Moringa peregrina (Forssk.) Fiori belongs to the third group. Sparse genetic studies of the genus or family confirm the real existence of this species relative to the other species of the genus, especially with regard to the Indian Moringa, Moringa oleifera Lam. (See in particular the articles: Olson, ME 2002, Combining Data from DNA Sequences and Morphology for a Phylogeny of Moringaceae (Brassicales), Systematic Botany 27 (1): 55-73; Hassanein, AMA and Al-Soqee, AA, 2018, Morphological and genetic diversity of Moringa oleifera and Moringa peregrina genotypes, Horticulture, Environment and Biotechnology 59 (2): 251-261). A recent article on Moringa oleifera sampled from different locations in Saudi Arabia concluded that the species is genetically stable (Alaklabi, A., 2015, Genetic diversity of Moringa peregrina species in Saudi Arabia with ITS sequences, Saudi Journal of Biological Sciences 22: 186-190), but has a high level of genetic variation within the population.

[0009] The species Moringa peregrina is found in rocky environments in Yemen, Oman, Saudi Arabia, East Africa, Sudan, Ethiopia, Eritrea, Somalia and Djibouti. Its presence in Iran seems to be limited to the southeastern provinces, but this needs to be confirmed (PROTA14 = Munyanziza E. and Yongabi KA, Vegetable oils / Oleaginous plants, Moringa peregrina (Forssk.) Fiori, http: / / database.prota.org / protahtml / moringa peregrina_fr.htm, accessed on 10 / 23 / 2019). In the Middle East and in Egypt, the species is now represented only by rare scattered relict stations (except for a few populations in the highlands), mainly in the Sudan region. Today, Moringa peregrina is also considered rare and endangered in Sudan and Yemen. Relative to other species of its branches, Moringa peregrina occupies the driest and harshest habitats. It is apparently more drought-resistant than the large-scale commercially planted Moringa in tropical and subtropical regions. Recent studies have shown that seed size and girth have a favorable effect on germination time and growth rate and speed of young individuals (Gomaa NH and Picó F.X., 2011, Seed germination, seedling traits, and seed bank of the tree Moringa peregrina (Moringaceae) in a hyper-arid environment, American Journal of Botany 98 (6): 1024-1030), indicating that resource allocation has been adjusted for seed quality rather than quantity, which enables Moringa arabicus to reproduce efficiently in extreme (ultra-dry) abiotic environments. In terms of cell layers, Moringa arabicus seeds have a central mesotesta that is thicker than those of Moringa oleifera.

[0010] There are some historical reports that tend to indicate that Moringa oil was actively traded in the Al-Ula region during the early Islamic period (Naseef, AAS, 1995, Al-'Ulā, A study of Cultural and Social Heritage). Locally produced oil from Moringa is now primarily destined for personal consumption or for the local market. In Saudi Arabia, the leaves are traditionally used as an internal decoction for the treatment of diabetes, intestinal diseases, eye diseases and anemia (Abdel-Kader, MS, Hazazi AMA, Elmakki OA and Alqasoumi SI, 2018, A survey on the traditional plants used in Al Kobah village, Saudi Pharmaceutical Journal 26(6):817-821) and as a diuretic, erythematous and astringent (Aqeel AAM, Tariq M., Mossa JS, Al-Yahya MA and Al-Said MS, 1984, "Plants used in Arabian Folk medicine", Report submitted to Saudi Arabian National Centre for Science and Technology, Riyadh, Saudi Arabia). In Oman, women use the oil extracted in late summer to combat migraines, fevers, burns, lacerations and fractures, constipation and stomach aches, as well as muscle pains, dry hair and labor pains (Ghazanfar S.A., 1994, Handbook of Arabian Medicinal Plants, 1 sted., CRC Press, Boca Raton, Ann Arbor, US; Ghazanfar SA, 1998, Plants of Economic Importance, cap.15, in Ghazanfar, SA and Fisher, M. (ed.) Vegetation of the Arabian Peninsula. Geobotany25, pages 241-264, Kluwer Academic Publishers, table11.1, page 247and 11.7page251). It is also used in aromatic compositions (Ghazanfar SA, 1998, page 259) and as a facial wash in Oman and Yemen (Ghazanfar SA and Rechinger B., 1996, Two multi-purpose seed oils from Oman. Plants for Food and Medicine. Paper presented at the joint meeting of the Society for Economic Botany and International Society for Ethnopharmacology, London, July 1-7, 1996).

[0011] Traditional uses of Moringa arabicum seed oil include treating headaches, fever, constipation, burns, abdominal pain, back and muscle pain, and labor pain (Boulos, L., 2000. Flora of Egypt, vol. I. Al Hadara Publishing, Cairo, p. 238; El Batran, SA, Abdel-Salam, OM, Abdelshfeek, KA, Nazif, NM, Ismail, SI, Hammouda, FM, 2005. Phytochemical and pharmacological investigation on Moringa peregrina (Forssk) Fiori., Nat. Prod. Sci. 11 (3), 199-206.). Other traditional uses of the seeds include cooking and curing abdominal pain (Van der Vossen, HAM, Mkamilo, GS, 2007. PROTA 14: Vegetable Oils. PROTA publishers, Wageningen, pp. 119-120).

[0012] Lalas et al. (Lalas, S., Gortzi, O., Athanasiadis, V., Tsaknis, J, and Chinou, I., 2012, Determination of Antimicrobial Activity and Resistance to Oxidation of Moringa peregrina Seed Oil, Molecules 17, p. 2330-2334) found that Moringa peregrina seed oil was active against Staphylococcus aureus, S. epidermidis, Pseudomonas aeruginosa, Escherichia cloacae, E. coli, Klebsellia pneumoniae, Candida albicans, C. tropicalis, and C. glabrata. The MICs of these microorganisms were 3.5, 3.35, 4.38, 4.8, 4.3, 4.95, 5.7, 3.3, and 3.25 mg / ml, respectively.

[0013] Tsaknis (Tsaknis, J., 1998. Characterization of Moringa peregrina Arabiaseed oil. Grassas y Aceites 49 (2), 170-176) studied the chemical composition of Moringa peregrina Arabiaseed oil and found that palmitic acid and stearic acid were the main components of saturated FA, while oleic acid and gadoleic acid were the main unsaturated FA. Among the sterols, β-sitosterol was the most important, and there were also campesterol, stigmasterol and rapeseed sterol. α-, γ- and δ-tocopherol were also detected.

[0014] Osman and Abohassan (2012) also showed that of the nine fatty acids identified in the seeds, five were classified as saturated fatty acids, and the highest content was oleic acid (53.94%-64.19%), palmitic acid (12.89%-17.88%), and stearic acid (6.63%-11.51%). Another comprehensive analysis of seed oil by Somali et al. (Somali, MA, Bajneid, MA, Alfhaimani, SS, 1984. Chemical composition and characteristics of M. peregrina seeds and seed oil. Journal of the American Oil Chemists' Society 61 (1), 85-86) showed that unsaturated fatty acids accounted for 84.7% of the oil. The authors summarize this unprecedented discovery as follows: "Moringa arabica seeds contain 1.8% moisture, 54.3% oil, 22.1% protein, 3.6% fiber, 15.3% carbohydrates, and 2.5% ash. The composition and characteristics of the extracted oil were determined. Gas-liquid chromatography of fatty acid methyl esters showed the presence of 14.7% saturated fatty acids and 84.7% unsaturated fatty acids. The fatty acid composition was as follows: palmitic acid 9.3%, palmitoleic acid 2.4%, stearic acid 3.5%, oleic acid 78.0%, linoleic acid 0.6%, linolenic acid 1.6%, arachidonic acid 1.8%, and behenic acid 2.6%.

[0015] Other studies on Moringa peregrina seed oil from Iran (Gharibzahedi, SMT, Ansarifard, I., Hasanabadi, Y., Sadeghi, H., Ghahderijani, M., and Yousefi, R., 2013. Physicochemical properties of Moringa peregrina seeds and its oil. Quality Assurance and Safety Crops and Food 5(4), 303-309) also showed similar data: oleic acid (77.9%) was the main fatty acid, followed by palmitic acid (9.3%), stearic acid (3.5%) and behenic acid (2.6%). All these data and others (e.g., Salaheldeen, M., Aroua, MK, Mariod, AA, Cheng, SF, and Abdelrahman, MA, 2014. An evaluation of Moringa peregrina seeds as a source for bio-fuel, Industrial Crops and Products 61, 49-61) indicate that polyunsaturated fatty acids are the main components of Moringa peregrina seed oil.

[0016] The vitamin composition of Moringa peregrina seeds was studied (Asghari, G., Palizban, A., and Bakhshaei, B., 2015. Quantitative analysis of the nutritional components in leaves and seeds of the Persian Moringa peregrina (Forssk.) Fiori, Pharmacognosy Research 7 (3), 242-248). It was recorded as: sufficient amount of vitamin C: 14.0 ± 0.6 mg / 100 g / dry weight; and vitamin A: 24.8 ± 0.7 mg / 100 g / dry weight. In addition, elemental analysis in the seeds showed that the calcium content was 1164.8 ± 43.4 mg / 100 g / dry weight, and the potassium content was 572 ± 10 mg / 100 g / dry weight.

[0017] The composition of the oil of Moringa peregrina and Moringa oleifera from Saudi Arabia and Egypt was studied by Robiansyah et al. in 2014 (Robiansyah, I., Hajar, AS, Al-kordy, MA, and Ramadan, A. 2014. Current Status of Economically Important Plant Moringa peregrina (Forrsk.) Fiori in Saudi Arabia: A Review, International Journal of Theoretical and Applied Sciences 6 (1), 79-86; table 1, p. 81 & table 2 & 3, p. 82). The oil has a high tocopherol content, which is composed of α-, β- and δ-tocopherols, with concentrations of 145, 58 and 66 mg / kg, respectively. This high content of tocopherol will act as a protective agent for the oil during storage and processing. Other studies have shown that Moringa seed oil can withstand up to 10.5 hours at 120°C, which is much higher than extra virgin olive oil (8.9 hours).

[0018] Cosmetic compositions comprising extracts derived from Moringa seeds are known in the cosmetics field. For example, document US667047 describes an oxidatively stable emollient produced from Moringa oil or its derivatives. It discloses a composition having high slip characteristics comprising Moringa long-chain oil having less than 1% methylene interrupted unsaturation.

[0019] Document KR101540333 discloses a mixed extract prepared by extracting a mixture of Moringa oleifera, Dictamnus albus and Annona muricata species with purified water; extracting at 100°C for 6 hours, and then filtering the obtained extract. The obtained filtered mixed extract is used for preventing hair loss.

[0020] Document KR101947705 discloses a cosmetic composition, a pharmaceutical composition and a food composition for preventing hair loss or promoting hair growth, comprising an extract of Saussurea lappa and Caesalpinia sappan obtained by extraction with ethanol, as well as Moringa oil and black cumin seed oil. The composition comprises 0.01 to 1% by weight of Moringa oil, and preferably 0.01%, the use being due to its mechanical lubricating activity. The Moringa species described in the document, according to its geographical origin, appears to be Moringa, as described in the abstract.

[0021] Finally, document KR102277088 discloses a cosmetic composition comprising a fermentation product of camellia oil, argan oil and moringa oil and a preparation method thereof, which are used as active ingredients for improving the scalp or preventing hair loss.

[0022] All the cited prior art discloses compositions for preventing or treating hair loss made from mixtures of different extracts, including extracts of the species Moringa oleifera. No document discloses that Moringa oil can be used alone.

[0023] In view of the foregoing, one problem that the present invention proposes to solve is to develop novel products based on natural extracts of Moringa arabicum, such as Moringa arabicum oil, which can be used in cosmetics or medicines and can be easily used to improve capillary density or prevent and treat hair loss.

[0024] The applicant has found that a composition comprising, as an active ingredient, oil extracted from Moringa arabica seeds makes it possible to significantly improve the capillary density of the scalp or skin.

[0025] Applicants have discovered that this novel composition is particularly effective for regrowth of hair and / or slowing down hair loss. Summary of the invention

[0026] A first aspect of the present invention is a cosmetic topical composition for improving capillary density, comprising as active ingredient at least one oily extract of Moringa oleifera seeds, optionally in combination with cosmetically acceptable excipients.

[0027] A second aspect of the present invention is a method for preparing a composition according to the present invention, according to which the method comprises the following steps:

[0028] a) drying the hulled seeds of Moringa oleifera (Forssk.) Fiori harvested when the fruit is ripe to obtain an internal moisture content of less than 8%, preferably less than or about 6%, even more preferably about 4%,

[0029] b) pressing the dry hulled seeds obtained in step a) with a mechanical endless screw press in order to separate the oil of the dry hulled seeds from the rest of the dry hulled seeds, thereby obtaining on the one hand virgin oil of the dry hulled seeds and on the other hand cake,

[0030] c) said virgin oil of the dried hulled seeds obtained in step b) is then naturally decanted, separated and placed in sterile containers and optionally combined with cosmetically acceptable excipients.

[0031] A third aspect of the invention is a cosmetic treatment for hair, characterised in that it comprises applying to the scalp and / or hair an effective amount of at least about 0.06 g of a composition according to the invention twice a day for at least 5 minutes, preferably 10 minutes, and optionally rinsing with water and / or conventional shampoo.

[0032] A fourth aspect of the present invention is a dermatological composition for preventing and treating hair loss, containing as an active ingredient at least one oily extract of Moringa arabica seeds, optionally with a dermatologically acceptable excipient.

[0033] The present invention will be better understood, and other objects, details, features and advantages of the present invention will become more apparent, in the following description of several specific embodiments of the present invention, which are given by way of illustration only and not limitation. DETAILED DESCRIPTION

[0034] In this description, unless otherwise stated, it is understood that when a range is given, it includes the upper and lower limits of the range.

[0035] In the present invention, the following definitions apply:

[0036] - "Topical composition": an active ingredient according to the invention or a composition comprising said active ingredient is applied or spread onto the surface of the skin or scalp or hair.

[0037] - “Cosmetically or dermatologically acceptable”: suitable for topical use, in contact with human skin, without any risk of toxicity, incompatibility, instability or allergic response.

[0038] - The expression "preventing hair loss" as used herein refers to minimizing hair loss, preventing hair loss due to various habits and environmental influences such as genetic causes, hormone imbalance, mental stress of social life, exposure to air pollution and intake of processed foods.

[0039] - The expression "treatment of hair loss" as used herein means minimizing the loss of hair and causing hair to grow in areas where it would normally be present but is not present due to various diseases and treatments that lead to hair loss.

[0040] - "Shelled seeds": means seeds that are harvested with the husk or pericarp remaining around the seed.

[0041] - "When the fruit is ripe": means that the fruit is ripe, preferably when the pod starts to split and turns from dark beige to brown, and when the lower quarter of the pod twists 180° so that the valves open.

[0042] - The expression "improving the capillary density of hair": means preventing hair loss, stimulating hair growth and increasing hair density, i.e. per cm2 The amount of hair is higher than before the beauty treatment.

[0043] - "About": plus or minus 10% to 20% relative to given information (duration, percentage, etc.).

[0044] - "Active ingredient" or "active agent": a sufficient amount of an extract according to the invention to obtain the described biological activity. Depending on preventive or therapeutic reasons, the amount of active agent may vary in a proportion of 5% to 100% by weight relative to the total weight of the composition.

[0045] - "Less than": By "less than", it is meant strictly less than. When no minimum value is specified, "less than X" means included in the range between 0 and X, excluding X. For example, "less than 8%" means 0% to 8% inclusive, excluding 8%; "less than 25 μm" means 0% to 25 μm inclusive, excluding 25 μm; "5% to less than 100%" means 5% to 100%, including 5%, excluding 100%.

[0046] A first aspect of the present invention is a cosmetic topical composition for improving capillary density, comprising as an active ingredient an oily extract of Moringa arabica seeds, optionally in combination with a cosmetically acceptable excipient.

[0047] Due to its characteristics, the oily extract of Moringa arabica seeds has been shown to be particularly effective for improving capillary density, hair regrowth and / or slowing down hair loss.

[0048] Depending on the embodiment, such a composition may include one or more of the following features.

[0049] According to one embodiment, the composition contains virgin oil of Moringa arabica seeds as active ingredient.

[0050] According to another embodiment, the composition contains winterized oil of Moringa arabica seeds as an active ingredient. This winterized oil is described in Example 1 below.

[0051] According to a preferred embodiment, the seeds are shelled seeds of Moringa oleifera (Forssk.) Fiori harvested when the fruit is ripe and then dried to obtain an internal moisture content of less than 8%, preferably less than or about 6%, even more preferably about 4%. The shelled seeds can also be dried to obtain an internal moisture content of less than 4%.

[0052] Generally, the in-hull seeds are dried to obtain an internal moisture content comprised between 2% and 8%.

[0053] According to one embodiment, the composition contains 5 to 100% by weight of an oily extract of Moringa oleifera seeds.

[0054] According to another embodiment, the composition contains 100% by weight of an oily extract of Moringa arabica seeds. In this embodiment, the oily extract of Moringa arabica seeds does not contain any excipients or ingredients other than the natural ingredients extracted from the seeds. In this case, the composition includes 100% Moringa arabica oil. The oily extract may be the virgin oil. In another embodiment, the oily extract is the winterized oil.

[0055] According to one embodiment, the composition contains between 5% and less than 100% by weight of an oily extract of Moringa oleifera seeds and other ingredients for improving the formulation, ie, adjusting the formulation to another formulation more suitable for administration.

[0056] Because composition according to the present invention is intended for topical administration, said composition optionally contains the acceptable medium of cosmetics, i.e. a medium compatible with skin and skin appendages, and encompasses all cosmetic forms. These compositions can be in the following form in particular: emulsifiable paste, oil-in-water or water-in-oil emulsion or composite emulsion, slurry, solution, suspension, gel, milk, lotion, stick or even powder, and can be applicable to being applied to skin and / or skin appendages such as hair. These compositions comprise the necessary excipients for their preparations, such as solvents, emollients, thickeners, diluents, surfactants, antioxidants, bioactivators, dyes, preservatives and spices.

[0057] The composition according to the invention may consist especially of a hair care composition and in particular a shampoo, a hair conditioner, a treatment lotion, a styling cream or gel, a hair conditioning lotion, a hair mask, etc. The cosmetic composition according to the invention may be used especially in treatments involving an application that may or may not be followed by rinsing, or alternatively in the form of a shampoo.

[0058] The compositions according to the invention may also include any additives usually used in the envisaged field of application and also adjuvants required for their formulation, such as solvents, thickeners, diluents, antioxidants, dyes, sunscreens, pigments, fillers, preservatives, fragrances, odor absorbers, cosmetic or pharmaceutical active agents, essential oils, vitamins, essential fatty acids, surfactants, film-forming polymers, etc.

[0059] The INCI Dictionary and Handbook ("International Nomenclature of Cosmetic Ingredients" (13th Edition, 2010), published by Personal Care Products Council Inc., Washington, DC) describes a wide variety of cosmetic and pharmaceutical ingredients commonly used in the skin care industry, but are not limited thereto, which are suitable for use as additional ingredients in the compositions according to the present invention.

[0060] In any case, the skilled person will take care to ensure that these adjuvants and their proportions are chosen so that the desired beneficial properties of the composition according to the invention are not adversely affected.

[0061] A second aspect of the present invention is a method for preparing a composition according to the present invention, according to which the method comprises the following steps:

[0062] a) drying the hulled seeds of Moringa oleifera (Forssk.) Fiori harvested when the fruit is ripe to obtain an internal moisture content of less than 8%, preferably less than or about 6%, even more preferably about 4%,

[0063] b) pressing the dry hulled seeds obtained in step a) with a mechanical screw press in order to separate the oil contained in the dry hulled seeds from the rest of the dry hulled seeds, thereby obtaining on the one hand virgin oil of the dry hulled seeds and on the other hand cake,

[0064] c) said virgin oil of the dried hulled seeds obtained in step b) is then separated and placed in a sterile container. It is optionally combined with a cosmetically acceptable excipient.

[0065] In order to obtain the virgin oil of the dried hulled seeds, step b) comprises carrying out solid / liquid separation. This solid / liquid separation can be carried out by various processes, such as natural decantation, centrifugation and / or filtration.

[0066] According to another embodiment, the method for preparing the composition according to the invention comprises the following steps:

[0067] - carrying out steps a) and b) as described above to obtain virgin oil of the dried shelled seeds of Moringa oleifera,

[0068] - in step c), subjecting said virgin oil of the dried hulled seeds obtained in step b) to a first crystallization at about 13° C. under moderate stirring for 60 to 180 minutes, depending on the batch size, followed by a first vacuum filtration at about 13° C. and with a filtration cut-off of about 25 μm or less, preferably less than 10 μm, preferably about 5 μm, to obtain a first winterized oil of dried hulled seeds,

[0069] - in step d), the first winterized oil obtained in step c) is subjected to a second crystallization at about 4° C. under moderate stirring for 60 to 180 minutes depending on the batch size, followed by a second vacuum filtration at about 4° C. with a filter cutoff of about 25 μm or less, preferably less than 10 μm, preferably about 5 μm, to obtain a second winterized oil of dry hulled seeds,

[0070] - isolating, in step e), said second winterized oil obtained in step d) and placing it in a sterile container and optionally combining it with a cosmetically acceptable excipient.

[0071] Generally speaking, said filter cut-off of the first and second vacuum filtrations is comprised between 4 and 30 micrometers (μm).

[0072] A third aspect of the invention is a method for cosmetic treatment of hair comprising applying to the scalp and / or hair an effective amount of at least about 0.06 g of a composition according to the invention twice a day for at least 5 minutes, preferably 10 minutes, and optionally rinsing with water and / or conventional shampoo.

[0073] A fourth aspect of the present invention is a dermatological composition for preventing and treating hair loss, containing as an active ingredient at least one oily extract of Moringa arabica seeds, optionally with a dermatologically acceptable excipient.

[0074] According to one embodiment, the dermatological composition is further used to prevent and relieve itching.

[0075] The dermatological composition is then used as a medicament for preventing or treating hair loss or as a medicament for preventing or alleviating itching.

[0076] According to another embodiment, the dermatological composition contains virgin or winterized oil of Moringa arabica seeds.

[0077] According to another preferred embodiment, the seeds are shelled seeds of Moringa arabicum (Forssk.) Fiori, harvested when the fruit is ripe and then dried to obtain an internal moisture content of less than 8%, preferably less than or about 6%, even more preferably about 4%. The shelled seeds are dried to an internal moisture content comprised between 2% and 8%.

[0078] According to an embodiment, the dermatological composition contains 5 to 100% by weight of the oily extract of Moringa oleifera seeds.

[0079] Although the present invention has been described in conjunction with several specific embodiments, it is apparent that the present invention is not limited thereto in any way, and that the present invention includes all technical equivalents of the described manner and combinations thereof if they fall within the scope of the present invention.

[0080] Use of the verb "to comprise", "to contain" or "to include" and its conjugations does not exclude the presence of other elements or steps than those stated in a claim.

[0081] In the claims, any reference signs placed between parentheses shall not be construed as limiting the claim.

[0082] Example

[0083] Example 1: Preparation of plant extract from Moringa arabica seeds

[0084] The hulled seeds of Moringa arabicum (Forssk.) are dried in Fiori, harvested when the fruit is ripe, to obtain an internal moisture content of less than 8%, preferably less than 6%. The internal moisture can be about 6%, preferably about 4%. It is included between 2 and 8%.

[0085] They are then pressed with a mechanical screw press in order to separate the oil from the rest of the seed, thereby obtaining virgin oil on the one hand, and cake on the other hand.

[0086] The virgin oil is recovered after solid / liquid separation and / or additional filtration.

[0087] The virgin oil is subjected to a first crystallization for 60 to 180 minutes at about 13°C under moderate stirring (depending on the batch size), followed by vacuum filtration at about 13°C, with a filtration cutoff included between 4 and 30 μm, preferably about 25 μm or less than 25 μm, preferably less than 10 μm, preferably about 5 μm, to obtain the first winterized oil. The first filtrate, i.e. the first winterized oil, is subjected to a second crystallization for 60 to 180 minutes at about 4°C under moderate stirring (depending on the batch size), followed by vacuum filtration at about 4°C, with a filtration cutoff included between 4 and 30 μm, preferably about 25 μm or less than 25 μm, preferably less than 10 μm, preferably about 5 μm, to obtain the second winterized oil. The second winterized oil is then separated and placed in a sterilized container. It constitutes the winterized oil. Thereafter, the winterized oil is referred to as WINTERIZED ALULA PEREGRINA OIL.

[0088] Example 2: Using Aramo ASG 200F measures hair density

[0089] The use test was carried out in a laboratory under the control of a dermatologist. The study aimed to:

[0090] To evaluate the effects of cosmetic products on the tolerability of the application site after routine application of winterized ALULAPEREGRINA oil, according to the intended and designated duration of use;

[0091] Provide evidence of the activity of winterized ALULA PEREGRINA oil by conducting studies to confirm or exclude the properties claimed by the cosmetic product.

[0092] The questionnaire format, group characteristics and number of subjects met the requirements. The subjects applied winterized ALULA PEREGRINA oil according to the declared method of use.

[0093] The statement is considered confirmed only if the ratio of positive responses received to the total number of subjects participating in the study is above 50%.

[0094] A description of the test subjects is given in Table 1 .

[0095] [Table 1]

[0096]

[0097] Eligible subjects received the tested winterized ALULA PEREGRINA oil, a specially developed questionnaire and were obliged to:

[0098] Use the product according to the instructions.

[0099] Any other products with similar effects may not be used during the test.

[0100] · Conduct a detailed evaluation of the test products by using the questionnaires received,

[0101] If any side effects occur on the skin, they should stop using the product immediately and consult a specialist

[0102] Test results may be affected by the following factors:

[0103] · Type and condition of the site where the product is to be applied,

[0104] Genetic characteristics between individuals,

[0105] · The individual preferences of the subjects.

[0106] The purpose of this study was to evaluate the direct effect of the winterized ALULA PEREGRINA oil of the invention according to Example 1 on hair density. The test was carried out using a special measuring device manufactured by ARAM HUVIS Co., Ltd. - ARAMO ASG 200F. Instrumental studies have been carried out on 21 subjects. Measurements at 60 times magnification were carried out on the designated areas - before product application (D0), after 56 days of regular use (D56) and 84 days (D84). Each subject must apply twice a day, in the morning and evening, 3 drops of winterized ALULA PEREGRINA oil each time (one drop is about 0.02 g).

[0107] The study was conducted in an air-conditioned room at a temperature of 20 ± 2°C and a relative humidity of 50 ± 10%. Table 2 below gives the average results of hair density at the product application site measured before application (D0), after 56 days (D56) and 84 days (D84) of regular use, in units of [average hair volume / cm 2 ].

[0108] [Table 2]

[0109]

[0110] Conclusion: The composition comprising winterized ALULA PEREGRINA oil (winterizing oil) improved hair density by about 10% (average) after 56 days of regular use and by about 16% (average) after 84 days of regular use.

[0111] Example 3: Evaluation of the Effect of Compositions on Administration Site Tolerance

[0112] Based on medical examinations and interviews collected from the subjects, the test composition according to Example 1 (winterized ALULA PEREGRINA oil) was found to be very well tolerated at the site of application. In all subjects who completed the study, during routine application, in the interviews, no negative symptoms and sensations that could indicate intolerance to any component of the product, such as irritation, burning, redness or itching, occurred. The product did not cause dryness at the site of application in any subject.

[0113] Based on the results of the use tests, the properties of the composition winterized ALULA PEREGRINA oil were evaluated as shown in Table 3 below.

[0114] [Table 3]

[0115]

[0116] Conclusion: The results obtained in the tests allow to conclude that the composition according to the invention, used as intended, is very well tolerated by the test population having no contraindications to its use.

[0117] It can also be concluded that the composition helps relieve itching, as for 70% of the testers, the product soothed itching.

[0118] Under the conditions of the study, after 84 days of regular application, it was concluded that the composition winterized ALULA PEREGRINA oil:

[0119] Very well tolerated at the site of application,

[0120] Claimed properties have been confirmed based on a subjective questionnaire:

[0121] - Hair is softer after application.

[0122] -The product gives a protective feel to the hair.

[0123] -Leaves hair silkier after application.

[0124] -This product removes unpleasant hair odor.

[0125] -It leaves a neutral or even pleasant smell on the hair.

[0126] -The product gives a cleansed scalp feeling.

[0127] -The product soothes itching.

[0128] - Hair looks stronger after application.

[0129] -Hair looks longer after application.

[0130] -The product improves itching and a feeling of well-being.

[0131] -The product adds volume to hair.

[0132] -The product does not cause a dry feeling in the hair after application.

[0133] -This product has improved the texture of my hair.

[0134] -Hair looks moisturized after application.

[0135] -No change in hair color.

[0136] -The product restores shine to hair.

[0137] -I had no difficulty handling and using this product.

[0138] -I like the cosmetic qualities of this product.

[0139] Example 4: Comparative data on fatty acid profiles of Moringa oleifera and Moringa arabicum seed oils

[0140] [Table 4]

[0141]

[0142] Example 5: Hair Styling « Wax Gel » Hair Strengthener: Wet Hair Appearance Styling Product

[0143] [Table 5]

[0144]

[0145] Example 6: Stimulating Shampoo Hair Strengthener: Apply a thick layer and leave on for 5 to 10 minutes. Lather and wash with water rinse

[0146] [Table 6]

[0147]

[0148] Example 7: Anti-hair loss cream: Apply to scalp and massage in the morning and evening. Use a brush to style hair. If applied lengthwise, it will give a slightly wet look.

[0149] [Table 7]

[0150]

[0151] Example 8: Hair growth mask: Apply a thick layer on the scalp and leave it on for 5 to 10 minutes. Rinse with clean water.

[0152] [Table 8]

[0153]

[0154]

[0155] Example 9: Hair Strengthener Dry Oil: Hair care product. Apply to scalp and massage in morning and evening. Use a brush to If applied lengthwise, it creates a slightly wet look.

[0156] [Table 9]

[0157]

[0158] Example 10: Hair Strengthening Powder

[0159]

[0160]

[0161] Use after shampooing. Apply to scalp and leave for 5 to 10 minutes: Sprinkle on wet hair and massage into scalp

[0162] Allow the thick layer to stand for up to 10 minutes

[0163] Rinse with clean water.

Claims

1. A cosmetic topical composition for improving capillary density comprising as an active ingredient at least one oily extract of Moringa peregrina seeds.

2. The composition according to claim 1, characterized in that The oily extract of Moringa arabica seeds is combined with a cosmetically acceptable excipient.

3. The composition according to any one of claims 1 and 2, characterized in that The oily extract is virgin oil from Moringa arabica seeds.

4. The composition according to any one of claims 1 and 2, characterized in that The oily extract is winterized oil of Moringa arabicum seeds.

5. The composition according to any one of claims 1 to 4, characterized in that The seeds are shelled seeds of Moringa oleifera (Forssk.) Fiori which are harvested when the fruit is ripe and then dried to obtain an internal moisture content of less than 8%.

6. The composition according to claim 5, characterized in that The in-hull seeds are dried to obtain an internal moisture content of less than 6%.

7. The composition according to claim 6, characterized in that The hulled seeds were dried to obtain an internal moisture content of about 4%.

8. The composition according to any one of claims 1 to 7, characterized in that The composition contains 5% to 100% by weight of an oily extract of Moringa arabicum seeds.

9. The composition according to claim 8, characterized in that The composition contains 100% by weight of an oily extract of Moringa oleifera seeds.

10. The composition according to claim 8, characterized in that The composition contains between 5% and less than 100% by weight of an oily extract of Moringa arabica seeds and other ingredients for improving the formulation.

11. A method for preparing a composition according to any one of claims 1 to 3, characterized in that According to the method, it comprises the following steps: a) drying the hulled seeds of Moringa oleifera (Forssk.) Fiori harvested when the fruit is ripe to obtain an internal moisture content of less than 8%, b) pressing the dried, shelled seeds with a mechanical screw press in order to separate the oil from the rest of the seeds, thereby obtaining on the one hand virgin oil and on the other hand cake, c) The virgin oil obtained is then separated and placed in sterile containers.

12. The method according to claim 11, characterized in that In step c), the virgin oil is combined with a cosmetically acceptable excipient.

13. A method for preparing the composition according to claim 4, characterized in that According to the method, it comprises the following steps: a) drying the hulled seeds of Moringa oleifera (Forssk.) Fiori harvested when the fruit is ripe to obtain an internal moisture content of less than 8%, b) pressing the dried, shelled seeds with a mechanical screw press in order to separate the oil from the rest of the seeds, thereby obtaining on the one hand virgin oil and on the other hand cake, c) subjecting the virgin oil to a first crystallization at about 13° C. under moderate stirring for 60 to 180 minutes depending on the batch size, followed by vacuum filtration at about 13° C. with a filter cutoff of about 25 μm or less to obtain a first winterized oil, d) subjecting the first winterized oil to a second crystallization at about 4° C. with moderate stirring for 60 to 180 minutes depending on the batch size, followed by vacuum filtration at about 4° C. with a filter cutoff of about 25 μm or less to obtain a second winterized oil, e) The second winterized oil is then separated and placed in a sterilized container.

14. The method according to claim 13, characterized in that In step e), the second winterized oil is combined with a cosmetically acceptable excipient.

15. A method for hair beauty treatment, characterized in that: It comprises applying an effective amount of at least about 0.06 g of a composition according to any one of claims 1 to 10 to the scalp and / or hair twice a day for at least 5 minutes.

16. The method according to claim 15, characterized in that The effective amount is applied to the scalp and / or hair twice daily for 10 minutes.

17. The method according to any one of claims 15 and 16, characterized in that It includes the further step of rinsing with water and / or conventional shampoo.

18. A dermatological composition for preventing and treating hair loss, comprising as an active ingredient at least one oily extract of Moringa arabica seeds.

19. The dermatological composition according to claim 18, comprising a dermatologically acceptable excipient.

20. The dermatological composition according to any one of claims 18 and 19, for use in preventing and alleviating itching.

21. The dermatological composition according to any one of claims 18 to 20, characterized in that The oily extract is virgin oil or winterized oil of Moringa arabicum seeds.

22. The dermatological composition according to any one of claims 18 to 21, characterized in that The seeds are shelled seeds of Moringa oleifera (Forssk.) Fiori which are harvested when the fruit is ripe and then dried to obtain an internal moisture content of less than 8%.

23. The dermatological composition according to claim 21, characterized in that It contains 5 to 100% by weight of an oily extract of Moringa arabica seeds.

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