A cherry fruit tortrix sex attractant and a method for preparing the same

Abstract

The present application belongs to the technical field of insect control, and particularly relates to a Cydia funebrana sex attractant and a preparation method thereof. The present application aims to provide a new option for controlling Cydia funebrana. The technical scheme of the present application is a Cydia funebrana sex attractant, which comprises sex pheromone, synergist and solvent, the ratio of the sex pheromone to the synergist is 100:10-30; the solvent is at least one of n-heptane, n-hexane, silicone oil and paraffin oil; the sex pheromone comprises (Z)-8-dodecene acetate (Z8-12:Ac) and (E)-8-dodecene acetate (E8-12:Ac), and the mass ratio of the two is (85-90):(15-10); and the synergist is n-tetradecyl acetate (N-14:Ac). The present application provides a brand new Cydia funebrana sex attractant, which can be used for efficiently trapping Cydia funebrana.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of insect control, and particularly relates to a Cydia funebrana sex attractant and a preparation method thereof. BACKGROUND

[0002] Cydia funebrana belongs to Lepidoptera Tortricidae, and currently only Japan, South Korea, China and Russia have reported it. Cydia funebrana has been identified as an important pest of Japanese apple and plum fruits, and also causes damage to pear and peach fruits. With the increase of plum orchard area in South Korea, the damage of Cydia funebrana is also increasing. China first reported in 1979 that Cydia funebrana is a new fruit tree pest, but because Cydia funebrana has a very close genetic relationship with Cydia pomonella, the interspecific similarity of COI genes of the two species is 95.0% to 95.6%, and the interspecific similarity of COII genes is 94.6 to 95.5%. The external morphology and biological characteristics are very similar to those of Cydia pomonella, and it is difficult to distinguish them with the naked eye, so that the damage loss caused by Cydia funebrana is misjudged as being caused by Cydia pomonella. Japan and South Korea have conducted in-depth research on Cydia funebrana, and found that its host selection, oviposition habit and damage characteristics have some differences from those of Cydia pomonella, and have their own characteristics. The research on the biology and ecology of Cydia funebrana and the mechanism of disaster is less in China. Due to the high similarity of the external morphology of the two pests and the partial overlap of the occurrence period, Cydia funebrana is monitored and controlled as Cydia pomonella in production. However, in fact, the proportion of Cydia funebrana collected by Cydia pomonella sex pheromone is limited, which cannot accurately reflect the actual occurrence dynamics of Cydia funebrana in the field, resulting in monitoring error of Cydia funebrana and great discount of control effect. The use of sex pheromone for monitoring and biological control of agricultural pests is an important means of current green pest control. At present, there is a blank of Cydia funebrana sex pheromone attractant in China, which is difficult to meet the demand of modern agricultural green pest control in fruit orchard. Cydia funebrana has evolved for many years, and there may be genetic differentiation among different geographical populations. The development of suitable sex pheromone attractant for domestic population can accurately identify and distinguish Cydia funebrana, which is beneficial to better study the host range, ecological niche distribution and occurrence regularity of Cydia funebrana, and helps to reduce the damage loss of Cydia funebrana to plum, apple, peach and pear fruits, and promote the healthy development of the industry. SUMMARY

[0003] The technical problem to be solved by the present application is to provide a new choice for preventing and controlling Cydia funebrana.

[0004] The technical scheme of the present application is a kind of Cydia funebrana sex attractant, including sex pheromone, synergist and solvent, the mass ratio of solvent and (sex pheromone and synergist) is 1:1;The mass ratio of sex pheromone and synergist is 100:10~30;The solvent is at least one of n-heptane, n-hexane, silicone oil and paraffin oil;The sex pheromone includes (Z)-8-dodecene acetate (Z8-12:Ac) and (E)-8-dodecene acetate (E8-12:Ac), and the mass ratio of the two is 80~95:5~20;The synergist is n-tetradecyl acetate (N-14:Ac) or (Z)-8-tetradecenyl acetate (Z8-14:Ac).

[0005] Among them, the mass ratio of sex pheromone and synergist is 100:30.

[0006] Further, the mass ratio of (Z)-8-dodecene acetate (Z8-12:Ac) and (E)-8-dodecene acetate (E8-12:Ac) is 85~90:10~15.

[0007] Preferably, the mass ratio of (Z)-8-dodecene acetate (Z8-12:Ac) and (E)-8-dodecene acetate (E8-12:Ac) is 85:15.

[0008] Preferably, the purity of the sex pheromone is more than 90.00%.

[0009] Preferably, the purity of the synergist is more than 90.00%.

[0010] The present application also provides a preparation method of Cydia funebrana sex attractant, comprising the following steps:

[0011] S1, mix sex pheromone with solvent to obtain sex pheromone solution;

[0012] S2, mix synergist with solvent to obtain synergist solution;

[0013] S3, mix sex pheromone solution and synergist solution with equal volume to obtain attractant.

[0014] Further, in step S3, the attractant is placed in a releaser carrier.

[0015] Further, the releaser carrier is an ethylene slow-release bag, an ethylene slow-release bottle, an ethylene slow-release tube, an ethylene slow-release bottle, an ethylene slow-release rubber plug, a polyvinyl chloride slow-release bag, a polyvinyl chloride slow-release bottle, a polyvinyl chloride slow-release tube, a polyvinyl chloride slow-release bottle or a polyvinyl chloride slow-release rubber plug.

[0016] The application provides a new type of Cydia funebrana L. sex attractant, can adjust the amount of sex pheromone in a limited range according to the sensitivity of different geographical populations of Cydia funebrana L. to the sex pheromone, so that the optimal trapping level is achieved, and the effect of the sex attractant can be maintained or improved by replacing the synergist component. The application can select one of an ethylene slow-release bag, a polyvinyl chloride slow-release bag, a bottle, a tube, a bottle and a rubber plug as a slow-release carrier. The application can effectively monitor the occurrence law of Cydia funebrana L. in an orchard, clearly determine the occurrence period and reflect the occurrence dynamics. Most importantly, the application can be applied to the green control of fruit boring pests, improve the green control effect, reduce the use of chemical pesticides and be friendly to the environment. DETAILED DESCRIPTION

[0017] All reagents in the following examples are purchased from the Macron Fine Chemicals Company, and the purity of sex pheromone and synergist is more than 90.00%.

[0018] Example 1: Preparation of the Cydia funebrana L. sex attractant

[0019] (1) Sex pheromone (Z)-8-dodecenyl acetate (Z8-12:Ac) and (E)-8-dodecenyl acetate (E8-12:Ac) are dissolved in n-hexane, and the sex pheromone and n-hexane are mixed in a mass ratio of 1 mg:1 mg to obtain a sex pheromone mixture;

[0020] (2) The synergist n-tetradecyl acetate and n-hexane are mixed in a mass ratio of 0.3 mg:1 mg to obtain a synergist mixture;

[0021] (3) The sex pheromone mixture and the synergist mixture are mixed in equal volumes to obtain the Cydia funebrana L. sex attractant.

[0022] (4) The sex attractant prepared by the above preparation method is added to a releaser carrier (an ethylene slow-release rubber plug), and after the solvent volatilizes, a Cydia funebrana L. sex pheromone attractant core is obtained.

[0023] Example 2: Field experiment of the Cydia funebrana L. sex attractant

[0024] The test area is located in Xintan Town, Suixian County, Yunnan Province (28°36'46''N, 104°8'23''E), has a long history of plum tree planting, and the total area is more than 100,000 mu. The test orchard area is not less than 2*667 m 2 .

[0025] The test has a total of 8 treatments with 3 repetitions, and the preparation method in the 8 treatments is shown in Example 1. The amount can be adjusted according to the proportion in Table 1.

[0026] Each treatment is respectively:

[0027] Treatment one: Z8-12:Ac + E8-12:Ac (85:15);

[0028] Treatment two: Z8-12:Ac + E8-12:Ac (90:15);

[0029] Treatment three: Z8-12:Ac + E8-12:Ac (95:5);

[0030] Treatment four: Z8-12:Ac + E8-12:Ac (80:20);

[0031] Treatment five: Z8-12:Ac + E8-12:Ac (100:0);

[0032] Treatment six: Z8-12:Ac + E8-12:Ac (0:100);

[0033] Treatment seven: Z8-12:Ac + E8-12:Ac + N-14:Ac (85:15:30);

[0034] Treatment eight: Z8-12:Ac + E8-12:Ac + Z8-14:Ac (85:15:1).

[0035] Three repetitions are respectively set at 500 m, 700 m, 900 m altitude area, and each area is one repetition.

[0036] The installation method of the trap is as follows: in the middle area of the orchard, 8 adjacent trees are randomly selected, and one trap is hung on each tree, and each trap is installed with one sex attractant (prepared according to the preparation method of the patent), numbered 1-8, corresponding to treatment one to treatment eight. The trap is selected as a boat-shaped adhesive type, and the adhesive board is white. The trap is uniformly set on the branch outside the south side of the tree crown, and the lower end is 1.2-1.5 m away from the ground (adjusted according to the branches).

[0037] The trapping and investigation time is as follows: the test is carried out twice at the peak of the withered shoot and the fruit near the mature period. Five days after the installation of the trap, the number of adult insects trapped in each trap is counted and recorded manually. The results are shown in Table 1. From the data in the table, it can be seen that the sex attractant of the invention for the codling moth has an attracting effect of more than 95% (the ratio of the number of target insects trapped to the total number) on the codling moth in the plum orchard during the spring shoot period, and an attracting effect of more than 98% on the codling moth during the fruit harvesting period in the plum orchard; the sex attractant can be used for occurrence dynamic monitoring and trapping control of the codling moth in apple orchards, peach orchards, pear orchards and the like, and can achieve the purposes of labor saving, energy saving, economy and environmental protection.

[0038] Table 1 Trapping effect .

Claims

1. A Malacosoma neustria sex attractant, characterized by: The pheromone includes (Z)-8-dodecenyl acetate (Z8-12:Ac) and (E)-8-dodecenyl acetate (E8-12:Ac), and the mass ratio of the two is 80-95:5-20; the synergist is n-tetradecyl acetate (N-14:Ac) or (Z)-8-tetradecenyl acetate (Z8-14:Ac).

2. The C. cerasi sex attractant of claim 1, wherein: The mass ratio of the pheromone to the synergist is 100:

30.

3. The C. cerasi sex attractant of claim 1, wherein: The mass ratio of (Z)-8-dodecenyl acetate (Z8-12:Ac) to (E)-8-dodecenyl acetate (E8-12:Ac) is 85-90:10-15.

4. The C. cerasi sex attractant of claim 3, wherein: The mass ratio of (Z)-8-dodecenyl acetate (Z8-12:Ac) to (E)-8-dodecenyl acetate (E8-12:Ac) is 85:

15.

5. The C. cerasi sex attractant of claim 1, wherein: The purity of the pheromone is 90.00% or more.

6. The C. cerasi sex attractant of claim 1, wherein: The purity of the synergist is 90.00% or more.

7. A method of preparing the Cydia coarctata sex attractant of any one of claims 1 to 6, characterized by: The method comprises the following steps: S1, mixing the pheromone and the solvent to obtain a pheromone solution; S2, mixing the synergist and the solvent to obtain a synergist solution; S3, mixing the pheromone solution and the synergist solution in equal volumes to obtain the attractant.

8. The method of claim 7, wherein: In step S3, the attractant is placed in a releaser carrier.

9. The method of claim 8, wherein: The releaser carrier is an ethylene slow-release bag, an ethylene slow-release bottle, an ethylene slow-release tube, an ethylene slow-release bottle, an ethylene slow-release rubber plug, a polyvinyl chloride slow-release bag, a polyvinyl chloride slow-release bottle, a polyvinyl chloride slow-release tube, a polyvinyl chloride slow-release bottle, or a polyvinyl chloride slow-release rubber plug.