Sex pheromone composition for controlling spodoptera litura and application thereof

Abstract

This invention discloses a sex pheromone composition for controlling the Thai armyworm and its application. The composition comprises cis-11-hexadecene acetate and cis-11-hexadecene alcohol in a mass ratio of 3-3.6:0.4-1, with an optimal mass ratio of 5:1. This invention not only requires small dosages and is environmentally friendly, but can also be accurately applied to pest monitoring, mass trapping, and mating disruption, significantly improving the effectiveness of integrated pest management of the Thai armyworm and holding significant importance for its control.

Description

Technical Field

[0001] This invention belongs to the field of insect sex pheromone preparation technology, specifically relating to a sex pheromone composition for controlling the Taydipus tibetus and its application. Background Technology

[0002] The Thai armyworm (Odontestra attila Hreblay & Ronkay), belonging to the order Lepidoptera and family Noctuidae, is a leaf-eating pest. It primarily damages various plants, including sorghum, lavender, and clematis. Currently, control of the Thai armyworm mainly relies on chemical pesticides. The use of chemical pesticides has caused pollution to humans and the environment and has led to pesticide resistance in insects. Therefore, there is a need to develop green control technologies for the Thai armyworm.

[0003] Insect sex pheromones originate from the insect itself, causing no harm to humans or the environment, and are unlikely to induce pesticide resistance in pests. Therefore, insect sex pheromones are increasingly being used in fields to trap pests. Based on the principle that female insects release sex pheromones that strongly attract males of the same species, accurately determining the composition and proportion of female *Taymania taeniorhynchus* sex pheromones can be used for pest monitoring, mating disruption, and mass trapping, achieving the ideal effect of integrated pest management for *Taymania taeniorhynchus*. Currently, pheromone-trapping products are lacking for the monitoring and green control of *Taymania taeniorhynchus*. Summary of the Invention

[0004] In view of this, the purpose of this invention is to provide a sex pheromone composition for controlling the Taydipterus thunbergii, so as to improve the control effect of Taydipterus thunbergii.

[0005] To achieve the above objectives, the present invention provides a sex pheromone composition of the moth *Taydipterus xanthipes*, which is composed of cis-11-hexadecene acetate (Z11-16:Ac) and cis-11-hexadecene alcohol (Z11-16:OH) in a mass ratio of 3-3.6:0.4-1.

[0006] Preferably, the composition consists of cis-11-hexadecene acetate (Z11-16:Ac) and cis-11-hexadecene alcohol (Z11-16:OH) in a mass ratio of 3-8:1, more preferably 5:1.

[0007] A sex pheromone lure for the moth *Taydipterus taeniorhynchus* is prepared as follows: a hexane solution containing a *Taydipterus taeniorhynchus* pheromone composition is added dropwise to the lure. After the hexane solvent evaporates, the lure containing the *Taydipterus taeniorhynchus* sex pheromone is obtained. The prepared lure is then sealed in aluminum foil and refrigerated at -20°C.

[0008] The lure is made of rubber.

[0009] The aforementioned application of the *Taymania taeniorhynchus* pheromone lure in trapping *Taymania taeniorhynchus* involves suspending traps containing *Taymania taeniorhynchus* pheromone lures at different heights and intervals in areas where *Taymania taeniorhynchus* is present or likely to be present. This is used for monitoring *Taymania taeniorhynchus* infestations, mass trapping, and disrupting mating. The trap is a barrel-shaped trap.

[0010] Compared with existing technologies, the present invention has the following beneficial effects: the sex pheromone composition for the Thai armyworm provided by the present invention is simple to prepare and the raw materials are readily available; it has a strong trapping ability, a long-lasting effect, and the control effect can be maintained for more than 30 days; it is highly specific and will not cause the Thai armyworm to develop resistance; it is harmless to humans and animals and environmentally friendly; it has strong resistance and is easy to use. The present invention can be accurately applied to insect infestation monitoring, mating interference, and mass trapping, and is of great significance for the integrated management of the Thai armyworm. Detailed implementation method:

[0011] The specific embodiments of the present invention will be described in detail below, but it should be understood that the scope of protection of the present invention is not limited to the specific embodiments.

[0012] Example 1: Preparation of sex pheromones from the taeniacus 'Taiping' moth

[0013] A hexane solution containing the pheromone of the Taydipharma taurid was added dropwise to the lure. After the hexane solvent evaporated, a lure containing the Taydipharma taurid sex pheromone was prepared. The lure was placed in a barrel-shaped trap, which was then placed in areas with severe Taydipharma taurid infestations. To ensure release effectiveness, each rubber lure contained 200 μl of Taydipharma taurid sex pheromone or a combination thereof (167-1500 μg). The average spacing between traps was 10 m, and the trap height was set to 120 cm above the ground. To further illustrate the feasibility of this embodiment, nine specific formulations are given in Table 1:

[0014] Table 1

[0015]

[0016] Selected areas with severe Taydiplasma puja infestations, exceeding 10 mu (approximately 667 hectares), used each formulation with lures set 120 cm above the ground. Each treatment had three replicates. Barrel-shaped traps containing Taydiplasma puja pheromone lures were placed evenly and randomly, along with three blank barrel-shaped traps at 120 cm above the ground and three barrel-shaped traps containing three live, unmated female Taydiplasma puja molts (3 days after emergence). The average spacing between traps was 10 m. The number of Taydiplasma puja molts was assessed every two days after placement, and the live females were replaced. The trap locations were also randomly changed. The assessment continued for 30 days. The trapping results are shown in Table 2.

[0017] Table 2

[0018]

[0019]

[0020] The results show that the sex pheromone composition for *Taymania tamariscina* of the present invention has a good trapping effect on *Taymania tamariscina*. Significant comparison shows that cis-11-hexadecenyl acetate (Z11-16:Ac) or cis-11-hexadecenol (Z11-16:OH) alone has an attractant effect on *Taymania tamariscina*, but the effect is worse compared with the natural sex pheromone released by live female *Taymania tamariscina*. The trapping quantity treated with the composition of the present invention containing cis-11-hexadecenyl acetate (Z11-16:Ac) and cis-11-hexadecenol (Z11-16:OH) was significantly higher than that of the control group. The trapping effect is significantly better than the insect-attracting effect, indicating that cis-11-hexadecene acetate (Z11-16:Ac) and cis-11-hexadecene alcohol (Z11-16:OH) have a synergistic effect under the ratio of the present invention, which enhances the trapping ability against Taydipterus thaliana. Among them, the formulation 6-3 (1250 μg of cis-11-hexadecene acetate and 250 μg of cis-11-hexadecene alcohol (Z9, E12-14:Ac) has the best trapping effect against Taydipterus thaliana.

[0021] The sex pheromone composition of the *Taymania taeniorhynchus* provided by this invention can be accurately applied to the monitoring, mass trapping, and mating interference of *Taymania taeniorhynchus*, which is of great significance for the integrated management of *Taymania taeniorhynchus*.

[0022] The inventors used structurally similar trans-11-hexadecenyl acetate (E11-16:Ac) and trans-11-hexadecenol (E11-16:OH) as trapping agents, respectively, and found no trapping effect. This proves that the pheromones mentioned above have high specificity and cannot be replaced by their structural analogs.

Claims

1. The application of a sex pheromone composition in the biological control and pest monitoring of the Taydipterus thunbergii, wherein the sex pheromone composition is composed of cis-11-hexadecene acetate and cis-11-hexadecene alcohol in a mass ratio of 3-3.6:0.4-1.

2. In the application according to claim 1, the mass ratio of cis-11-hexadecene acetate to cis-11-hexadecene alcohol is 3-8:

1.

3. In the application according to claim 2, the mass ratio of cis-11-hexadecene acetate to cis-11-hexadecene alcohol is 5:

1.

4. The application according to any one of claims 1-3, using a trap containing a lure containing the sex pheromone composition to trap Taydipterus.

5. In the application according to claim 4, the trap is a barrel-shaped trap.

6. The application according to claim 5, wherein the method is as follows: in areas where Tay-no-Ophiopogon japonicus occurs or may occur, traps for monitoring the Tay-no-Ophiopogon japonicus, trapping large numbers of them and interfering with mating are suspended at different heights and at certain intervals.

7. In the application according to claim 4, the lure is prepared by the following method: a hexane solution of the sex pheromone composition is added dropwise to the lure, and the lure is obtained after the hexane solvent evaporates. The lure is then sealed in aluminum foil and refrigerated at -20°C.

8. In the application according to claim 7, the material of the lure core is rubber.

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