A millet foliar spray, a preparation method and application thereof

Abstract

The application discloses millet leaf surface spraying agent and a preparation method and application thereof. The millet leaf surface spraying agent comprises, in mass fractions, methyl jasmonate 15-18 parts, salicylic acid 6-9 parts and a surfactant 190-210 parts. The raw materials, i.e., methyl jasmonate, salicylic acid and the surfactant, are weighed according to proportions and uniformly mixed, and then diluted with water before use. The application realizes the improvement of millet quality by applying plant hormone methyl jasmonate and plant growth regulator salicylic acid and compounding with the surfactant. After the grain formation in the grain filling period of millet, the millet leaf surface spraying agent is sprayed, so that, compared with the millet without spraying, the content of millet xanthophyll, the content of yellow pigment and the content of total flavonoids after shelling are increased, the paste characteristics of millet powder are improved, and the quality of millet is improved as a whole.

Description

Technical Field

[0001] This invention relates to the field of fertilizer technology, and in particular to a foliar fertilizer spray for improving the quality of millet after hulling, its preparation method, and its application. Background Technology

[0002] Millet has strong tolerance to poor soil and drought, and is widely planted in dryland agricultural ecological zones, making it a strategic reserve crop for ensuring food security.

[0003] Hulled millet contains abundant functional nutrients, such as lutein, yellow pigments, and total flavonoids, and has unique advantages in balancing the nutritional structure of residents' diets.

[0004] Therefore, further increasing the content of the above-mentioned nutrients is of great significance for improving the overall quality of millet. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a millet foliar spray, its preparation method and application, so as to improve the lutein content, yellow pigment content, total flavonoid content and gelatinization characteristics of millet after hulling.

[0006] To solve the above technical problems, according to one aspect of the present invention, a millet foliar spray is provided, comprising, by weight parts, 15-18 parts of methyl jasmonate, 6-9 parts of salicylic acid, and 190-210 parts of surfactant.

[0007] Furthermore, by mass, it includes 16 parts of methyl jasmonate, 8 parts of salicylic acid, and 200 parts of surfactant.

[0008] Furthermore, the surfactant is polyethylene glycol octylphenyl ether (Triton X-100).

[0009] According to another aspect of the present invention, a method for preparing the millet foliar spray is provided, wherein methyl ester, salicylic acid and surfactant are weighed in proportion and mixed evenly; and diluted with water before use.

[0010] Furthermore, the dilution factor was 2 × 10⁻⁶. 5 times.

[0011] According to another aspect of the present invention, the application of the above-described millet foliar spray in improving the lutein content, yellow pigment content, total flavonoid content and gelatinization characteristics of millet is provided.

[0012] Furthermore, the millet foliar spray is applied to the leaves after the grains have formed during the millet filling stage.

[0013] Furthermore, the number of spraying applications is 3 to 4.

[0014] This invention improves the quality of millet by applying the plant hormone methyl jasmonate and the plant growth regulator salicylic acid, and by compounding them with surfactants.

[0015] Spraying the millet foliar spray described in this invention after grain formation during the grain-filling stage of millet results in increased lutein, yellow pigment, and total flavonoid content in the hulled millet compared to unsprayed millet, and also improves the gelatinization characteristics of millet flour, thus enhancing the overall quality of millet. Detailed Implementation

[0016] The millet foliar spray provided in a typical embodiment of the present invention comprises, by weight, 15-18 parts of methyl jasmonate, 6-9 parts of salicylic acid, and 190-210 parts of surfactant.

[0017] Methyl jasmonate is a plant hormone, and salicylic acid is a plant growth regulator. The addition of a surfactant increases the emulsifying ability between the spray components, allowing them to mix more effectively, adhere to the leaf surface, reduce the spray's flow and release rate, increase the spray's coverage area, and enhance the foliar application effect. The surfactant is preferably polyethylene glycol octylphenyl ether (Triton X-100).

[0018] When preparing the millet foliar spray, weigh out the raw materials methyl ester, salicylic acid, and surfactant according to the specified ratio and mix them evenly. Dilute with water before use; the preferred dilution ratio is 2 × 10⁻⁶. 5 times.

[0019] After the grains have formed during the grain-filling stage of millet, the millet foliar spray is applied to the leaves, preferably 3 to 4 times.

[0020] The technical solutions claimed in this invention will be further described below through some embodiments. However, the embodiments and comparative examples are for explaining the implementation schemes of this invention and do not exceed the scope of the subject matter of this invention. The scope of protection of this invention is not limited by the embodiments. Unless otherwise specified, the materials and reagents used in this invention can be obtained from commercially available products in the art.

[0021] The methyl jasmonate, salicylic acid, and surfactant used in the following examples were all purchased from Sigma-Aldrich (Shanghai) Trading Co., Ltd. Example 1

[0022] Planting Datong 29. The raw material composition of the foliar spray is: 16 kg of methyl jasmonate, 8 kg of salicylic acid, and 200 kg of surfactant. The above raw materials are mixed evenly and diluted 2×10 before use. 5Apply 0.5 L per square meter four times after the grains have formed during the millet's grain-filling stage. Example 2

[0023] Planting Datong 29. The raw material composition of the foliar spray is: 15 kg of methyl jasmonate, 6 kg of salicylic acid, and 210 kg of surfactant. The above raw materials are mixed evenly and diluted 2×10 before use. 5 Apply 0.5 L per square meter three times during the grain-filling stage of millet, after grain formation. Example 3

[0024] Planting Datong 29. The raw material composition of the foliar spray is: 17 kg of methyl jasmonate, 7 kg of salicylic acid, and 190 kg of surfactant. The above raw materials are mixed evenly and diluted 2×10 before use. 5 Apply 0.5 L per square meter four times after the grains have formed during the millet's grain-filling stage.

[0025] Comparative Example 1

[0026] Plant Datong 29. Spray the leaves four times with distilled water during the grain-filling stage of millet, after the grains have formed, without adding any reagents, at a rate of 0.5 L per square meter.

[0027] Comparative Example 2

[0028] The only difference from Example 1 is that it is diluted 6 × 10⁻⁶ before use. 5 times.

[0029] Comparative Example 3

[0030] The only difference from Example 1 is that the raw material composition is: 16 kg of methyl jasmonate and 200 kg of surfactant are mixed evenly, and salicylic acid is not included.

[0031] Comparative Example 4

[0032] The only difference from Example 1 is that the raw material composition is: 8 kg of salicylic acid and 200 kg of surfactant mixed evenly, without containing methyl jasmonic acid. Example 4

[0033] Planting Jingu 21. The raw material composition of the foliar spray is: 16 kg of methyl jasmonate, 8 kg of salicylic acid, and 200 kg of surfactant. The above raw materials are mixed evenly and diluted 2×10 before use. 5 Apply 0.5 L per square meter four times after the grains have formed during the millet's grain-filling stage. Example 5

[0034] Planting Jingu 21. The raw material composition of the foliar spray is: 18 kg of methyl jasmonate, 9 kg of salicylic acid, and 205 kg of surfactant. The above raw materials are mixed evenly and diluted 2×10 before use. 5 Apply 0.5 L per square meter three times during the grain-filling stage of millet, after grain formation. Example 6

[0035] Planting Jingu 21. The raw material composition of the foliar spray is: 16 kg of methyl jasmonate, 8 kg of salicylic acid, and 195 kg of surfactant. The above raw materials are mixed evenly and diluted 2×10 before use. 5 Apply 0.5 L per square meter four times after the grains have formed during the millet's grain-filling stage.

[0036] Comparative Example 5

[0037] Planting Jingu 21. Spray with distilled water four times during the grain-filling stage of millet, after grain formation, without adding any reagents, at a rate of 0.5 L per square meter.

[0038] Comparative Example 6

[0039] The only difference from Example 4 is that it is diluted 6 × 10⁻⁶ before use. 5 times.

[0040] Comparative Example 7

[0041] The only difference from Example 4 is that the raw material composition is: 16 kg of methyl jasmonate and 200 kg of surfactant are mixed evenly, and salicylic acid is not included.

[0042] Comparative Example 8

[0043] The only difference from Example 5 is that the raw material composition is: 8 kg of salicylic acid and 200 kg of surfactant mixed evenly, without containing methyl jasmonic acid.

[0044] The effects of the fertilizers prepared in Comparative Examples 1-8 and Examples 1 and 4 were verified as follows:

[0045] Experimental location: Dongyang Experimental Base of Shanxi Agricultural University (37°33′21″N, 112°40′2″E, altitude 800m).

[0046] Experimental crops: millet (varieties: Jingu 21 and Datong 29).

[0047] Planting area: Each treatment area was foliar sprayed with the comparative examples 1-8 and examples 1 and 4 respectively. Three replicate experimental fields were set up in a completely randomized block design. Each experimental field was 6 m long and 5 m wide. Fertilization methods and daily management were carried out according to local customs.

[0048] After the millet matured and was hulled, the contents of lutein, zeaxanthin, yellow pigment, total flavonoids and total polyphenols in the millet were determined. The results are shown in Table 1.

[0049] Table 1

[0050] Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 1 Example 4 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 5 Lutein 8.90 7.90 7.65 7.30 7.25 29.78 26.88 26.58 25.28 25.24 Zeaxanthin 2.98 2.91 2.62 2.35 2.26 8.75 8.28 8.31 8.21 8.28 Yellow pigment (μg / g) 7.76 6.82 6.83 6.78 6.81 20.10 18.51 18.75 18.48 18.52 Total flavonoids (mg / 100g) 67.90 74.77 58.65 52.36 48.43 89.26 91.62 86.26 82.35 77.95 Total polyphenols (mg / 100g) 48.75 45.03 44.23 44.08 44.11 56.98 51.25 50.48 50.52 50.20

[0051] As can be seen from Table 1, compared with Comparative Examples 1 and 5, which were not treated with foliar spray, the application of the foliar spray described in Examples 1 and 4 of this invention significantly increased the content of lutein, yellow pigment, total flavonoids and total polyphenols in millet.

[0052] Compared with Comparative Examples 2 and 6, which applied lower concentrations of foliar spray, after applying the foliar sprays described in Examples 1 and 4 of this invention, the total flavonoid content in millet decreased slightly, while the lutein content, yellow pigment content, and total polyphenol content in millet significantly increased.

[0053] Compared with Comparative Examples 3, 4, 7, and 8, which do not contain methyl jasmonate or salicylic acid, the application of the foliar sprays described in Examples 1 and 4 of this invention significantly increased the content of lutein, yellow pigment, total flavonoids, and total polyphenols in millet.

[0054] The cooking quality of millet was determined by hulling mature millet and using gelatinization characteristics as an indicator. The results are shown in Table 2.

[0055] Table 2

[0056] Gelatinization characteristics Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 1 Example 4 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 5 Gelatinization start time (min) 9.76 10.03 9.89 9.82 10.16 10.4 10.2 10.1 10.2 9.96 Gelatinization temperature (°C) 78.5 79.4 78.4 79.2 79.5 80.6 79.8 79.4 79.5 79.1 Peak viscosity (BU) 158 161 163 160 162 222 211 208 205 202 Final viscosity (BU) 283 278 276 274 275 452 442 438 435 430 Disintegration Value (BU) 40 41 45 41 42 55 48 47 43 41 Recovery Value (BU) 165 158 158 155 155 285 279 277 273 269 Peak-valley viscosity (BU) 118 120 118 119 120 167 163 161 162 161

[0057] As can be seen from Table 2, compared with Comparative Examples 1 and 5, which were not sprayed with foliar spray, the application of the foliar spray described in Examples 1 and 4 of this invention significantly improved the final viscosity and recovery value of millet paste.

[0058] Compared with Comparative Examples 2 and 6, which applied lower concentrations of foliar spray, the application of the foliar sprays described in Examples 1 and 4 of this invention further improved the final viscosity and recovery value of millet paste.

[0059] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A millet foliar spray, characterized in that: By mass, it includes 15-18 parts of methyl jasmonate, 6-9 parts of salicylic acid, and 190-210 parts of surfactant; the surfactant is polyethylene glycol octylphenyl ether (Triton X-100).

2. The millet foliar spray according to claim 1, characterized in that: By weight, it includes 16 parts methyl jasmonate, 8 parts salicylic acid, and 200 parts surfactant.

3. The method for preparing the millet foliar spray according to claim 1 or 2, characterized in that: Weigh out methyl jasmonate, salicylic acid, and surfactant in the specified proportions and mix thoroughly; dilute with water before use.

4. The preparation method according to claim 3, characterized in that: The dilution factor is 2×10. 5 times.

5. The application of the millet foliar spray according to claim 1 or 2 in improving the content of lutein, yellow pigment, total flavonoids and gelatinization characteristics of millet.

6. The application according to claim 5, characterized in that: The millet foliar spray is applied to the leaves after the grains have formed during the grain-filling stage of millet.

7. The application according to claim 6, characterized in that: Spray 3 to 4 times.