Rice bran fatty alkanol composition for reducing fat and losing weight and preparation method thereof

Abstract

The invention provides a rice bran fatty alkanol composition for reducing fat and losing weight and a preparation method of the rice bran fatty alkanol composition. The rice bran fatty alkanol composition for reducing fat and losing weight is composed of rice bran fatty alkanol and soybean sterol, and the rice bran fatty alkanol accounts for 5-30% of the mass of the composition. By optimizing the fingerprint spectrum of rice bran fatty alkanol, the composition proportion of higher fatty alkanol is effectively improved, the lipid-lowering, weight-losing and liver-protecting effects can be effectively improved, the lipid-lowering, weight-losing and liver-protecting effects are greatly improved by matching with soybean sterol, and a tablet containing the composition is further developed. The obtained lipid-lowering weight-losing liver-protecting tablet is safe and free of side effects, and can be eaten for a long time to prevent obesity and fatty liver.

Description

Technical Field

[0001] The invention relates to a rice bran fatty alkanol composition for reducing fat and weight, and in particular to the technical field of fat-reducing products. Background Art

[0002] Obesity has become a major disease that affects health. Studies have found that obesity increases the risk of diabetes, cardiovascular disease, hypertension, hyperlipidemia, fatty liver, various tumors, metabolic disorders and other diseases. Effectively reducing and controlling fat content is the key to improving the public's health level.

[0003] For the intervention and treatment of obesity, the current lifestyle intervention combining exercise therapy and diet therapy is more effective and the most recommended, but it is inefficient, has a long cycle, and is difficult for patients to adhere to; surgical intervention is also one of the effective means of weight loss, but it is often high-risk and expensive, which is difficult for patients to afford; in addition, drug treatment is efficient, effective, low-risk, and easy for patients to accept, but some drugs may have severe adverse reactions to patients. Therefore, it is urgent to develop new natural ingredients for lipid-lowering functional foods for the prevention and treatment of obesity. Summary of the invention

[0004] In view of this, the object of the present invention is to provide a rice bran fatty alkanol composition for reducing blood lipids and losing weight. Rice bran fatty alkanol is rich in resources and has rich health benefits. It is a naturally occurring higher fatty alcohol, the main component of which is octacosanol, which is derived from rice bran wax and is a new type of food raw material. Rice bran fatty alkanol has unique physiological functions such as bidirectional regulation of blood lipids, enhancement of endurance and anti-fatigue, and liver protection. The present invention combines rice bran fatty alkanol and soybean sterols to develop a new type of lipid-lowering and weight-loss product.

[0005] The present invention is achieved by adopting the following technical solutions:

[0006] A rice bran fatty alkanol composition for reducing fat and weight consists of rice bran fatty alkanol and soybean sterol, wherein the rice bran fatty alkanol accounts for 5-30% of the mass of the composition.

[0007] Preferably, the method for preparing rice bran fatty alkanol of the present invention comprises the following steps:

[0008] Add 3 times the volume of anhydrous ethanol and 7 times the volume of No. 200 solvent oil and 0.12 times the mass of sodium hydroxide to 10 kg of refined rice bran wax, heat to boiling in a water bath, stir and reflux for 4 hours, wash 3 times with 5 times the volume of hot water, remove the lower aqueous phase, wash until the pH is neutral, cool the supernatant overnight, and vacuum filter to obtain a filter cake;

[0009] The filter cake is desolventized and dried to obtain crude saponified rice bran wax alcohol;

[0010] Under the conditions of working pressure of 100-150Pa, kettle temperature of 240-300°C and reflux ratio of 4:1-12:1, the rice bran wax saponification crude alcohol is distilled to collect fractions of 236-237°C, 240-242°C and 247-248°C respectively; the above three fractions are mixed in a mass ratio of 10:65:25 to obtain rice bran fatty alkanol.

[0011] Preferably, the mass ratio of rice bran fatty alkanol to soybean sterol in the rice bran fatty alkanol composition is 7:93.

[0012] Preferably, the mass ratio of rice bran fatty alkanol to soybean sterol in the rice bran fatty alkanol composition is 13:87.

[0013] Preferably, the mass ratio of rice bran fatty alkanol to soybean sterol in the rice bran fatty alkanol composition is 23:77.

[0014] The preparation method of the rice bran fatty alkanol composition for reducing fat and weight of the invention is to uniformly mix the rice bran fatty alkanol and soybean sterol according to a certain proportion.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The present invention optimizes the fingerprint of rice bran fatty alkanols, effectively improves the composition ratio of higher fatty alkanols, can effectively improve the lipid-lowering, weight-loss and liver-protecting effects, and is combined with soybean sterols to greatly increase the lipid-lowering, weight-loss and liver-protecting effects. A tablet containing the composition is further developed. The obtained lipid-lowering, weight-loss and liver-protecting tablet is safe and has no side effects, and can be taken for a long time to prevent obesity and fatty liver. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 The gas phase fingerprint of rice bran fatty alkanol in Example 1;

[0018] Figure 2 This is a comparison chart of the body weight and total weight gain of hamsters in each group at the end of the experiment;

[0019] Figure 3 This is a comparison of the weights of the liver and adipose tissue of each group of hamsters at the end of the experiment;

[0020] Figure 4 This is a comparison chart of blood lipid indexes of hamsters in each group at the end of the experiment;

[0021] Figure 5 This is a comparison chart of serum liver function indicators of hamsters in each group at the end of the experiment. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical scheme and advantages of the present invention clearer, the preferred embodiments of the present invention are further described in detail in conjunction with the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Examples 1-3 and Comparative Examples 1-2 Lipid-lowering and Weight-loss Compositions

[0024] Table 1. Formula of lipid-lowering and weight-loss composition

[0025]

[0026] 1. Preparation of rice bran fatty alkanol: Take 10 kg of refined rice bran wax (purchased from Gaoan Qinghe Grease Co., Ltd.), add 3 volumes of anhydrous ethanol, 7 volumes of No. 200 solvent oil, and 0.12 times the mass of sodium hydroxide, heat to boiling in a water bath, stir and reflux for 4 hours, wash 3 times with 5 volumes of hot water, remove the lower aqueous phase, wash until the pH value is approximately equal to 7, cool the supernatant overnight, vacuum filter to obtain a filter cake, desolventize the filter cake, dry it, and obtain a crude saponification product of rice bran wax. Alcohol; under the conditions of working pressure of 100-150Pa, kettle temperature of 240-300℃, reflux ratio of 4:1-12:1, the prepared crude alcohol is distilled, and the fraction with the tower top temperature of 230-250℃ is collected at every 1-2℃ tower top temperature interval, and the fractions of 236-237℃, 240-242℃ and 247-248℃ are taken, and the three fractions are mixed at a mass ratio of 10:65:25 to obtain a rice bran fatty alkanol sample (POLI);

[0027] 2. Preparation of standard solution of higher fatty alkanol: Accurately weigh docosanol (C 22 ), tetracosanol (C 24 ), hexacosanol (C 26 ), octacosanol (C 28 ), triacontanol (C 30 ) standard, and 5 mg of rice bran fatty alkyl alcohol (POLI), were dissolved in n-hexane and fixed to volume with a 10 mL volumetric flask to prepare a 500 μg / mL solution;

[0028] 3. The GC chromatographic analysis conditions of rice bran fatty alkanols are as follows: detector: FID; chromatographic column: DB-5HT (30m×320μm×0.1μm); column temperature: 260℃ for 1 min, then rise to 300℃ at a rate of 5℃ / min, and maintain for 11min; vaporization chamber temperature: 300℃; detector temperature: 300℃; carrier gas (N2) flow rate: 25.0mL / min; hydrogen (H2) flow rate: 30.0mL / min; auxiliary gas (Air) flow rate: 400mL / min; injection volume: 1μL; split ratio: 10:1; qualitative analysis was performed using higher fatty alkanol standards, and the mass percentage of each peak was calculated using the area normalization method. The results are as follows: Figure 1 shown.

[0029] Example 4 Test of lipid-lowering activity of various lipid-lowering and weight-loss compositions

[0030] 56 golden hamsters (142±5g) were randomly divided into 7 groups according to their body weight: (1) normal diet control group (CON); (2) high-fat and high-cholesterol diet model group (HFC, 20% fat, 2% cholesterol); (3) comparative example 1 treatment group (HFC + 0.135% rice bran fatty alcohol); (4) comparative example 2 treatment group (HFC diet + 0.432% soybean sterol); Example 1 treatment group (HFC diet + 0.034% rice bran fatty alcohol + 0.432% soybean sterol); Example 2 treatment group (HFC diet + 0.068% rice bran fatty alcohol + soybean sterol); Example 3 treatment group (HFC diet + 0.135% rice bran fatty alcohol + 0.432% soybean sterol). Hamster rearing conditions: 12h / 12h, dark / light; 25±2℃, free access to food and free drinking water. The pre-test period was 1 week, and the basic diet was fed; the test period was 4 weeks, and the diets of each group were fed. At the end of the 4th week, the animals were killed and sampled, and serum, liver, fat (subcutaneous / abdominal / back fat) were collected, tissue weights were weighed, and serum TC, TG, HDL-C, LDL-C, ALT, AST and other indicators were tested. The results are shown in Table 2. Figures 2 to 5 .

[0031] Experimental Results

[0032] Table 2. Effects of rice bran fatty alkanols and sterols on body weight and body fat in hamsters

[0033]

[0034] Note: * represents P < 0.05, ** represents P < 0.01, *** represents P < 0.001

[0035] The results show that the lipid-lowering and weight-loss compositions of the present invention can reduce the final body weight, body weight gain, liver, subcutaneous fat, abdominal fat, back fat, and serum TG, TC, LDL-C, ALT, and AST values ​​in the serum of hamsters fed with a high-fat and high-cholesterol diet, and increase the HDL-C value. Among them, the final body weight (p=0.011), body weight gain (p=0.013), liver (p=0.002), subcutaneous fat (p<0.001), and serum TG (p=0.004), TC (p<0.001), and LDL-C (p<0.001) of Example 3 have extremely significant lipid-lowering, weight-loss, and liver-protecting effects; and the lipid-lowering, weight-loss, and liver-protecting effects of Examples 1 and 2 are second only to those of Example 3.

[0036] From Table 2, Figure 2-5 It can be seen that the lipid-lowering, weight-loss and liver-protecting effects of Comparative Examples 1 and 2 are less reduced than those of Examples 1-3. The results show that the combination of rice bran fatty alkyl alcohols and soybean sterols has a synergistic effect on indicators such as final body weight, body weight gain, liver weight, back fat, TG, TC, LDL-C, and HDL-C, which is more conducive to inhibiting the production of obesity and fatty liver induced by a high-fat diet, improving abnormal blood lipid levels caused by hyperlipidemia, and improving liver damage caused by a high-fat diet.

[0037] The above results show that the lipid-lowering and weight-loss composition of the present invention can effectively inhibit the absorption and deposition of body fat, protect the liver, and improve the abnormal blood lipid level caused by hyperlipidemia.

[0038] Embodiment 5-7

[0039] The lipid-lowering and weight-loss composition of Example 3 was prepared into lipid-lowering and weight-loss tablets according to Table 3.

[0040] Table 3

[0041]

[0042] Tablet preparation process

[0043] S1. Mixing: According to the formula, weigh rice bran fatty alkyl alcohol powder, soybean sterol powder, microcrystalline cellulose, pregelatinized starch, maltodextrin, magnesium stearate and gum arabic, and mix them with a blender. The stirring time is 20 min, the blender temperature is 25 ° C, and the ambient humidity is 40%;

[0044] S2. Tablet preparation: The mixed powder is compressed into tablets according to the formula to obtain tablets of about 1 g each.

[0045] The embodiments described above are part of the embodiments of the present invention, rather than all of the embodiments. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

Claims

1. A rice bran fatty alkanol composition for reducing blood lipids and weight, characterized in that: The rice bran fatty alkanol composition is composed of rice bran fatty alkanol and soybean sterol, and the rice bran fatty alkanol accounts for 5-30% of the mass of the composition.

2. The rice bran fatty alkanol composition for reducing blood lipids and losing weight according to claim 1, characterized in that: The preparation method of the rice bran fatty alkanol comprises the following steps: Add 3 times the volume of anhydrous ethanol and 7 times the volume of No. 200 solvent oil and 0.12 times the mass of sodium hydroxide to 10 kg of refined rice bran wax, heat to boiling in a water bath, stir and reflux for 4 hours, wash 3 times with 5 times the volume of hot water, remove the lower aqueous phase, wash until the pH is neutral, cool the supernatant overnight, and vacuum filter to obtain a filter cake; The filter cake is desolventized and dried to obtain crude rice bran wax saponification alcohol; Under the conditions of working pressure of 100-150Pa, kettle temperature of 240-300°C and reflux ratio of 4:1-12:1, the rice bran wax saponification crude alcohol is distilled to collect fractions of 236-237°C, 240-242°C and 247-248°C respectively; the above three fractions are mixed in a mass ratio of 10:65:25 to obtain rice bran fatty alkanol.

3. The rice bran fatty alkanol composition for reducing blood lipids and losing weight according to claim 1, characterized in that: The mass ratio of rice bran fatty alkanol to soybean sterol in the rice bran fatty alkanol composition is 7:

93.

4. The rice bran fatty alkanol composition for reducing blood lipids and losing weight according to claim 1, characterized in that: The mass ratio of rice bran fatty alkanol to soybean sterol in the rice bran fatty alkanol composition is 13:

87.

5. The rice bran fatty alkanol composition for reducing blood lipids and losing weight according to claim 1, characterized in that: The mass ratio of rice bran fatty alkanol to soybean sterol in the rice bran fatty alkanol composition is 23:

77.

6. The method for preparing the rice bran fatty alkanol composition for reducing blood lipids and weight according to any one of claims 1 to 5, characterized in that: Just mix the bran fatty alcohol and soybean sterol evenly according to the proportion.

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