Artemisia apiacea extract-containing plant oral care product capable of clearing fire, protecting gingiva and inhibiting bacteria

Abstract

The invention provides a plant oral care product capable of clearing away fire, protecting gum and inhibiting bacteria and containing an artemisia apiacea extract. The artemisia apiacea extract, a radix zanthoxyli extract, a liquorice root extract, an isatis tinctoria extract and a golden camellia extract are added into the oral care product as active ingredients; wherein the addition amount of the artemisia apiacea extract is 0.005%-5% of the total weight of the oral care product, the addition amount of the radix zanthoxyli extract is 0.05%-10% of the total weight of the oral care product, the addition amount of the licorice root extract is 0.01%-5% of the total weight of the oral care product, the addition amount of the isatis tinctoria extract is 0.01%-5% of the total weight of the oral care product, and the addition amount of the licorice root extract is 0.01%-5% of the total weight of the oral care product. The addition amount of the camellia nitidissima extract is 0.01%-5% of the total weight of the oral care product. According to the oral care product, various plant components capable of clearing heat, protecting gingiva and inhibiting bacteria are applied to the oral care product, dental plaque can be effectively inhibited, and oral symptoms such as excessive internal heat, red and swollen gingiva and gingival bleeding can be relieved.

Description

Technical Field

[0001] The invention relates to the field of oral care products, in particular to a plant fire-clearing, gum-protecting and antibacterial oral care product containing an Artemisia annua extract. Background Art

[0002] In recent years, according to the results of the national oral health survey, more than 90% of the surveyed people have been troubled by oral problems, among which caries, bad breath, and bleeding gums are the most frequent, followed by sensitivity, ulcers, redness and swelling, dry mouth, etc. The main reasons are irregular diet, excessive fatigue, staying up late and eating spicy food, etc., which cause oral problems such as bleeding gums, redness and swelling, dry mouth, and bad breath. People with oral problems generally tend to choose functional oral care products, and daily care is simpler and more convenient than going to the hospital for treatment.

[0003] At present, the main ingredients for solving common oral problems on the market are potassium nitrate, fluoride, antibacterial agents, tranexamic acid, etc. Although they can quickly solve the corresponding symptoms, long-term use or improper use can easily cause tolerance in the oral flora. Chinese herbal medicine has a long history of application, and it also has the advantages of rich sources, confirmed efficacy of ancient prescriptions, small toxic side effects, and not easy to produce drug resistance. Long-term use can not only relieve symptoms but also play a role in conditioning. Therefore, it is very necessary to study a Chinese herbal ointment that can effectively inhibit dental plaque and relieve oral symptoms such as oral inflammation, gingival redness and swelling, and gingival bleeding. Summary of the invention

[0004] The present invention provides a plant oral care product containing an Artemisia annua extract for clearing heat, protecting gums and inhibiting bacteria. A variety of plant ingredients for clearing heat, protecting gums and inhibiting bacteria are applied to the oral care product, which can effectively inhibit dental plaque and relieve oral symptoms such as oral inflammation, red and swollen gums, and bleeding gums.

[0005] To achieve the above object, the technical solution of the present invention is:

[0006] A plant oral care product containing an Artemisia annua extract for clearing heat, protecting gums and inhibiting bacteria, wherein the oral care product contains Artemisia annua extract, Zanthoxylum chinense extract, Licorice extract, Isatis indigotica extract and Camellia chrysantha extract as active ingredients; wherein: the Artemisia annua extract is added in an amount of 0.005% to 5% of the total weight of the oral care product, the Zanthoxylum chinense extract is added in an amount of 0.05% to 10% of the total weight of the oral care product, the Licorice extract is added in an amount of 0.01% to 5% of the total weight of the oral care product, the Isatis indigotica extract is added in an amount of 0.01% to 5% of the total weight of the oral care product, and the Camellia chrysantha extract is added in an amount of 0.01% to 5% of the total weight of the oral care product.

[0007] Preferably, the weight ratio of the Artemisia annua extract, the Zygophyllum chinensis extract, the Licorice root extract, the Isatis indigotica extract and the Camellia chrysantha extract is 1:4-40:1-5:1-5:0.2-5.

[0008] Preferably, the weight ratio of the Artemisia annua extract, the Zygophyllum chinensis extract, the Licorice root extract, the Isatis indigotica extract and the Camellia chrysantha extract is 1:4:1.5:1.5:1.

[0009] Preferably, the added amount of the Artemisia annua extract is 0.005% to 2% of the total weight of the oral care product.

[0010] Preferably, the added amount of the Zanthoxylum bungeanum extract is 0.05% to 8% of the total weight of the oral care product.

[0011] Preferably, the added amount of the licorice extract is 0.01% to 3% of the total weight of the oral care product.

[0012] Preferably, the added amount of the Isatis indigotica extract is 0.02% to 3% of the total weight of the oral care product.

[0013] Preferably, the added amount of the Camellia chrysantha extract is 0.01% to 3% of the total weight of the oral care product.

[0014] Preferably, the Artemisia annua extract is an ethanol extract; and / or the Zanthoxylum bungeana extract is an ethanol extract; and / or the Licorice root extract is an ethanol extract; and / or the Isatis indigotica extract is an ethanol extract; and / or the Camellia chrysantha extract is an ethanol extract.

[0015] Preferably, the oral care product is toothpaste, mouthwash, tooth powder, oral spray, oral rinse or chewing gum.

[0016] The Artemisia annua extract, Zanthoxylum bungeanum extract, Licorice extract, Isatis indigotica extract and Camellia chrysantha extract of the present invention are preferably extracted from raw medicinal materials, wherein:

[0017] Artemisia annua is the dried aerial part of Artemisia annual.L., a plant of the Asteraceae family. It tastes bitter and slightly pungent. It has antimalarial, antipyretic and antibacterial effects.

[0018] Zanthoxylum nitidum (Roxb.) DC., the dried root of the Rutaceae plant, tastes bitter and pungent, and is neutral in nature. It has the effects of promoting blood circulation and removing blood stasis, promoting qi and relieving pain, dispelling wind and unblocking meridians, detoxifying and reducing swelling, and can be used for injuries from falls, stomachache, toothache, rheumatic pain, etc.

[0019] Licorice, the dried root and rhizome of the leguminous plants Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L., is sweet and neutral in nature. It has the effects of tonifying the spleen and replenishing qi, relieving pain, moistening the lungs and relieving cough, clearing away heat and detoxifying. It can be used for sore throat, peptic ulcer, carbuncle and sore ulcer, etc.

[0020] Isatis indigotica Fort., the dried root of the cruciferous plant, is bitter and cold in nature. It has the effects of clearing away heat and detoxifying, cooling blood and removing spots, relieving sore throat and relieving pain, and can be used for sore throat, mouth ulcers, etc.

[0021] Camellia nitidissima CWChi, Theaceae, is a twin sister of tea, camellia, camellia nitidissima, camellia oleifera, and camellia sasanqua. Its flowers and leaves are unique medicinal plants in Guangxi, and are also plants with medicinal and edible properties. It has the effects of clearing away heat and detoxifying, promoting diuresis and removing dampness, stopping diarrhea, and stopping bleeding. It can be used for dysentery, sore throat, blood in stool, etc.

[0022] For Zanthoxylum bungeanum extract, licorice extract, Isatis indigotica extract and Camellia chrysantha extract, the present invention provides a preferred preparation method: the Zanthoxylum bungeanum extract, licorice extract, Isatis indigotica extract and Camellia chrysantha extract are respectively crushed with the corresponding original medicinal materials, extracted by heating and refluxing with ethanol, and then concentrated to a ratio of 0.5 to 2:1 by mass of the original medicinal materials.

[0023] For the Artemisia annua extract, the present invention provides a preferred preparation method: crushing the original Artemisia annua medicinal material, extracting it with ethanol cold soaking, concentrating, purifying, crystallizing and drying to obtain the Artemisia annua extract, wherein the purity of the Artemisia annua extract is ≥98%.

[0024] In the extraction method, for the extract of Zanthoxylum bungeanum, licorice extract, isatis indigotica extract, and camellia chrysantha extract, the original medicinal materials are crushed, and the dried plant original medicinal materials are crushed into particles, slices, or segments; extracted with ethanol, preferably extracted 1 to 3 times with a volume fraction of 60 to 95% ethanol aqueous solution of 6 to 10 times the weight of the original medicinal materials; concentrated preferably by reduced pressure concentration to obtain a concentrated liquid or extract. For the Artemisia annua extract, the preferred extraction method is: take the dried Artemisia annua branches and leaves and crush them, add 8 to 10 times the weight of 90 to 99% ethanol aqueous solution with a volume fraction of 2 to 3 times, and concentrate under reduced pressure to 0.5 to 2.5 times the weight of the original medicinal material, extract continuously with ethyl acetate, decolorize with activated carbon, filter, crystallize and recrystallize, and dry to obtain a powdered Artemisia annua extract. Of course, the extraction method of the original medicinal material is not limited to the above method, and other methods such as supercritical extraction and ultrasonic extraction can also be used for extraction.

[0025] The present invention adopts a variety of plant active ingredients such as Artemisia annua extract, Zanthoxylum bungeanum extract, Licorice extract, Isatis indigotica extract, Camellia chrysantha extract and the like in a specific ratio to prepare oral care products. The above ingredients work together. Antibacterial tests have shown that the oral care products of the present invention have a strong antibacterial effect on Staphylococcus aureus, Candida albicans and Escherichia coli, and have a strong inhibitory effect on the biological cell growth curves of Porphyromonas gingivalis and Streptococcus mutans; pharmacological tests have shown that the oral care products of the present invention also have significant hemostatic and anti-inflammatory effects, can relieve oral inflammation, reduce gum bleeding and swelling and other related problems, maintain gums, and maintain oral health. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a growth curve of Porphyromonas gingivalis.

[0027] Figure 2 This is the growth curve of Streptococcus mutans. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0029] A plant-based oral care product containing an Artemisia annua extract for clearing heat, protecting gums and inhibiting bacteria, wherein the oral care product contains an Artemisia annua extract, a Zanthoxylum bungeanum extract, a Licorice extract, an Isatis indigotica extract and a Camellia chrysantha extract as active ingredients; wherein: the amount of the Artemisia annua extract added is 0.005% to 5% (e.g., 0.005%, 0.1%, 1%, 2% or 5%) of the total weight of the oral care product, the amount of the Zanthoxylum bungeanum extract added is 0.05% to 10% (e.g., 0.05%, 0.1%, 1% , 4%, 8% or 10%), the added amount of the licorice extract is 0.01% to 5% (for example, 0.01%, 0.5%, 1%, 3% or 5%) of the total weight of the oral care product, the added amount of the isatis indigotica extract is 0.01% to 5% (for example, 0.01%, 0.02%, 0.5%, 3% or 5%) of the total weight of the oral care product, and the added amount of the camellia chrysantha extract is 0.01% to 5% (for example, 0.01%, 0.02%, 0.5%, 3% or 5%) of the total weight of the oral care product.

[0030] Preferably, the weight ratio of the Artemisia annua extract, the Zygophyllum chinensis extract, the Glycyrrhiza uralensis extract, the Isatis indigotica extract and the Camellia chrysantha extract is 1:4-40:1-5:1-5:0.2-5 (for example, 1:4:1:1:0.2, 1:10:1:2:1, 1:40:5:5:5, 1:4:1.5:1.5:1, 1:10:2:4:2, 1:20:2:2:2 or 1:10:3:3:5).

[0031] Preferably, the weight ratio of the Artemisia annua extract, the Zygophyllum chinensis extract, the Licorice extract, the Isatis indigotica extract, and the Camellia chrysantha is 1:4:1.5:1.5:1.

[0032] Preferably, the amount of the Artemisia annua extract added is 0.005% to 2% (eg, 0.005%, 0.1%, 0.5%, 1% or 2%) of the total weight of the oral care product.

[0033] Preferably, the added amount of the Zanthoxylum bungeanum extract is 0.05% to 8% (eg, 0.05%, 0.1%, 1%, 4% or 8%) of the total weight of the oral care product.

[0034] Preferably, the added amount of the licorice extract is 0.01% to 3% (eg, 0.01%, 0.5%, 0.8%, 1% or 3%) of the total weight of the oral care product.

[0035] Preferably, the added amount of the Isatis indigotica extract is 0.02% to 3% (eg, 0.01%, 0.02%, 0.1%, 0.5% or 3%) of the total weight of the oral care product.

[0036] Preferably, the added amount of the Camellia chrysantha extract is 0.01% to 3% (eg, 0.01%, 0.02%, 0.1%, 0.5% or 3%) of the total weight of the oral care product.

[0037] Preferably, the Artemisia annua extract is an ethanol extract; and / or the Zanthoxylum bungeana extract is an ethanol extract; and / or the Licorice root extract is an ethanol extract; and / or the Isatis indigotica extract is an ethanol extract; and / or the Camellia chrysantha extract is an ethanol extract.

[0038] Preferably, the oral care product is toothpaste, mouthwash, tooth powder, oral spray, oral rinse or chewing gum.

[0039] For Zanthoxylum bungeanum extract, licorice extract, Isatis indigotica extract, and Camellia chrysantha extract, this embodiment provides a preferred preparation method: the Zanthoxylum bungeanum extract, licorice extract, Isatis indigotica extract, and Camellia chrysantha extract are respectively crushed to the corresponding original medicinal materials, extracted by heating and refluxing with ethanol, and then concentrated to a ratio of 0.5 to 2:1 by mass of the original medicinal materials.

[0040] For the Artemisia annua extract, the present invention provides a preferred preparation method: crushing the original Artemisia annua medicinal material, extracting with ethanol cold soaking, concentrating, purifying, crystallizing and drying to obtain the Artemisia annua extract, wherein the purity of the Artemisia annua extract is ≥98%.

[0041] In the extraction method, for the extract of Zanthoxylum bungeanum, the extract of Licorice, the extract of Isatis indigotica, and the extract of Camellia chrysantha, the original medicinal materials are crushed, and the dried plant original medicinal materials can be crushed into particles, slices, or segments; extracted with ethanol, preferably extracted 1 to 3 times with a volume fraction of 60 to 95% (for example, 60%, 75%, 80% or 95%) of 6 to 10 times (for example, 6 times, 8 times, 9 times or 10 times) the weight of the original medicinal materials; concentrated preferably by reduced pressure concentration to obtain a concentrated liquid or extract, and for the extract of Artemisia annua, it is more preferred to take the dried Artemisia annua branches and leaves and crush them, add 8 to 10 times the weight of 90 to 99% ethanol aqueous solution with a volume fraction of 2 to 3 times, and concentrate under reduced pressure to 0.5 to 2.5 times the weight of the original medicinal materials, extract continuously with ethyl acetate, decolorize with activated carbon, filter, crystallize and recrystallize, and dry to obtain a powdered Artemisia annua extract. Of course, the extraction method of the dried original medicinal materials is not limited to the above method, and other methods such as supercritical extraction and ultrasonic extraction can also be used for extraction.

[0042] When preparing oral care products, artemisia annua extract, linden extract, liquorice extract, isatis indigotica extract and camellia chrysantha extract are added as hemostatic, anti-inflammatory and antibacterial ingredients.

[0043] The present invention will be further described below by way of examples, but the protection scope of the present invention is not limited to these embodiments.

[0044] In this embodiment, the preparation methods of Zanthoxylum indigoferum extract, Licorice extract, Isatis indigotica extract and Camellia chrysantha extract are as follows: crush the corresponding raw medicinal materials, extract them by heating and refluxing with 10 times the weight volume fraction of 75% ethanol for 3 hours, extract them 3 times, and then concentrate them under reduced pressure to 0.5 times the weight of the raw medicinal materials. The preparation method of Artemisia annua extract is as follows: crush the dried Artemisia annua branches and leaves, extract them by stirring and cold soaking with 10 times the volume fraction of 95% ethanol for 2 hours, extract them 3 times, and then concentrate them under reduced pressure to 0.5 times the weight of the raw medicinal materials, extract them continuously with solvents such as ethyl acetate, decolorize them with activated carbon, filter them, cool them for crystallization, recrystallize them, and dry them to obtain Artemisia annua extract.

[0045] 1. Botanical oral care products containing Artemisia annua extract, Zanthoxylum bungeanum extract, Licorice extract, Isatis indigotica extract, and Camellia chrysantha extract for clearing heat, protecting gums, and inhibiting bacteria (taking toothpaste as an example)

[0046] The toothpaste components and weight percentages in Examples 1 to 5 are shown in Table 1 below:

[0047] Table 1 Plant fire-clearing, gum-protecting and antibacterial toothpaste containing Artemisia annua extract

[0048]

[0049]

[0050] 2. The hemostatic effect of plant-based fire-clearing, gum-protecting and antibacterial toothpaste containing Artemisia annua extract, Zanthoxylum bungeanum extract, Licorice extract, Isatis indigotica extract and Camellia chrysantha extract

[0051] Thirty healthy New Zealand rabbits were selected per group, weighing 2.0-2.5 kg, both male and female.

[0052] ), toothpaste blank matrix group (toothpaste blank matrix 1g / rabbit), Yunnan Baiyao powder group (Yunnan Baiyao powder 1g / rabbit), toothpaste QH-1 group, toothpaste QH-2 group, toothpaste QH-3 group, toothpaste QH-4 group, toothpaste QH-5 group, toothpaste QH-6 group (corresponding to toothpaste 1g / rabbit). 30mg / kg of 3% sodium pentobarbital was slowly injected into the ear vein. After anesthesia, the rabbits were fixed on the operating table in supine position. A midline incision of about 6-8cm in length was made in the abdomen to expose and fix the liver. The liver tissue was cut off from the left lobe of the liver with surgical scissors, and the length, width and height of the liver tissue were about 1cm, 1cm and 1cm respectively. The wound surface showed jet-like bleeding. After wiping off the spurted blood, immediately apply 1g of the test sample to the wound surface, cover with gauze, and press with a 200g weight for 1min. After 1min, remove the weight and observe the hemostasis effect. Stop bleeding when there is no bleeding. If there is still bleeding, apply the test sample and continue to press. Observe every 30s and record the hemostasis time. If there is still bleeding after more than 3min, it is recorded as failure to stop bleeding, and the hemostasis time is recorded as >3min. After each sample test, wash away the residual test substance with 37℃ saline to make the wound bleed again, and then test the next test sample. Each rabbit was subjected to four liver local wound injury hemostasis test experiments. Each test sample was applied according to the Latin square procedure to reduce the influence of the difference in the incision of the liver local wound injury and the order of medication. A total of 20 rabbit liver local wound injury bleeding hemostasis experiments were conducted for each test sample. The hemostasis effect was recorded as a score F according to the reciprocal scoring method (10 / T). If there was no hemostasis for more than 3min, F had no score and was recorded as 0. The results are shown in Table 2.

[0053] Table 2 Hemostatic effect on local wound injury and bleeding in rabbit liver

[0054]

[0055] Note: Compared with starch group: #P<0.05, ##P<0.01, ###P<0.001, ▲P>0.05.

[0056] Compared with the toothpaste blank matrix group: *P<0.05, **P<0.01, ***P<0.001, △P>0.05.

[0057] The QH-1 toothpaste group, QH-2 toothpaste group, QH-3 toothpaste group, QH-4 toothpaste group, QH-5 toothpaste group, and QH-6 toothpaste group respectively use the ointments of Example 1, Example 2, Example 3, Example 4, Example 5, and Example 6.

[0058] The experimental results showed that the toothpaste QH-4 group, toothpaste QH-5 group, toothpaste QH-6 group and Yunnan Baiyao group all had obvious hemostatic effects on local wound injury and bleeding in rabbit liver.

[0059] 3. Anti-inflammatory effect of plant-based fire-clearing, gum-protecting and antibacterial toothpaste containing Artemisia annua extract, Zanthoxylum bungeanum extract, Licorice extract, Isatis indigotica extract and Camellia chrysantha extract

[0060] 60 healthy Kunming mice weighing 18-22g were selected and randomly divided into 7 groups, with 10 mice in each group. The experiment set up a blank control group (normal saline 0.1ml / mouse), a toothpaste blank matrix group (toothpaste blank matrix group 0.1g / mouse), a fluocinolone acetonide cream group (fluocinolone acetonide cream 0.1g / mouse), a toothpaste QH-1 group, a toothpaste QH-2 group, a toothpaste QH-3 group, a toothpaste QH-4 group, a toothpaste QH-5 group, and a toothpaste QH-6 group (corresponding to toothpaste 0.1g / mouse). During the experiment, xylene 0.1ml / mouse was evenly applied to the outer two sides of the right auricle of each animal in each group to cause inflammation, and the left ear was not treated as its own blank control. After applying xylene for 30 minutes, 0.1g / mouse of the test sample of each group was evenly applied to the inner and outer two sides of the right auricle of each animal in each group. One hour after the administration of the test substance, the mice were killed by cervical dislocation, the two auricles were cut off, the test substance sample of the right auricle was washed with physiological saline, wiped clean with dry cotton balls, the two auricles were overlapped, and two round ear pieces were punched out at the same position with a puncher with an inner diameter of 8mm. They were weighed on a FA1004 electronic analytical balance, and the swelling value was obtained by subtracting the weight of the left ear piece from the weight of the right ear piece. The data were subjected to statistical t test. The results are shown in Table 3.

[0061] Table 3 Effects of xylene on acute inflammation and swelling of the auricle of mice ( n=10)

[0062] Group Dosage (g / each) Auricular swelling value (mg) Blank control group 0.1ml / pc 16.71±3.85 Toothpaste blank matrix group 0.1 <![CDATA[16.43±3.58 ▲ <!-- 6 -->]]> Fluocinolone acetonide cream group 0.1 <![CDATA[10.56±2.33 ### ]]> Toothpaste QH-1 set 0.1 <![CDATA[15.63±3.47 ▲△ ]]> Toothpaste QH-2 set 0.1 <![CDATA[15.27±3.45 ▲△ ]]> Toothpaste QH-3 set 0.1 <![CDATA[14.55±3.12 ▲△ ]]> Toothpaste QH-4 set 0.1 <![CDATA[13.51±2.62 # *]]> Toothpaste QH-5 set 0.1 <![CDATA[12.87±2.56 # **]]> Toothpaste QH-6 set 0.1 <![CDATA[12.13±2.49 # **]]>

[0063] Note: Compared with the blank control group: #P<0.05, ##P<0.01, ###P<0.001, ▲P>0.05.

[0064] Compared with the toothpaste blank matrix group: *P<0.05, **P<0.01, ***P<0.001, △P>0.05.

[0065] QH-1 is Example 1, QH-2 is Example 2, QH-3 is Example 3, QH-4 is Example 4, QH-5 is Example 5, QH-6 is Example 6

[0066] The experimental results showed that the toothpaste QH-4 group, toothpaste QH-5 group, toothpaste QH-6 group and fluocinolone acetonide cream group could significantly reduce the weight of the swollen ear pinna of mice with acute inflammation, significantly inhibit the acute inflammation swelling of the ear pinna of mice caused by xylene, and had a significant anti-inflammatory effect on acute inflammation.

[0067] 4. Antibacterial effect of plant-based fire-clearing, gum-protecting and antibacterial toothpaste containing Artemisia annua extract, Zanthoxylum bungeanum extract, Licorice extract, Isatis indigotica extract and Camellia chrysantha extract

[0068] Dental plaque is a bacterial biofilm that is firmly attached to the surface of teeth. Bacteria are the main components of dental plaque, and there are many types of bacteria, including aerobic bacteria and anaerobic bacteria, mainly including Staphylococcus aureus, Candida albicans, Escherichia coli, Streptococcus mutans, Porphyromonas gingivalis, etc. The amount of dental plaque is closely related to the occurrence of oral diseases. The bacteria in dental plaque will produce acidic substances, which will lead to tooth demineralization and caries, and also cause oral diseases such as gingivitis and periodontal disease.

[0069] 1. Antibacterial experiment

[0070] (1) Aerobic bacteria inhibition test: The antibacterial effect test was carried out according to QBT 2738-2012 "Evaluation Method for Antibacterial Effect of Oral Cleaning and Care Products". The toothpaste experimental concentration was 1:4 under the conditions of (36±1)℃ and (28±1)℃, the action time was 3min, and the test bacteria were Staphylococcus aureus (ATCC 6538) ((36±1)℃), Escherichia coli (ATCC25922) ((36±1)℃), and Candida albicans (ATCC 10231) ((28±1)℃). The test was repeated 3 times; when the inhibition rate was 50%-90%, it was marked as having an antibacterial effect; when the inhibition rate was ≥90%, it was marked as having a strong antibacterial effect. The results are shown in Table 4.

[0071] (2) Anaerobic bacteria inhibition experiment: The antibacterial effect test was carried out using the biological growth curve monitoring method. The bacterial culture solution prepared with the effective content of the above-mentioned extraction composition in the toothpaste was used as the test group; the bacterial culture solution without the above-mentioned extraction composition was used as the control group. At 35°C-37°C, under anaerobic conditions, the OD value was measured once every 1 hour using an ultraviolet spectrophotometer to represent the concentration and growth of the tested bacteria. Among them, the measurement wavelength of Porphyromonas gingivalis (ATCC 33277) was 450nm, and the measurement wavelength of Streptococcus mutans (ATCC 25175) was 600nm. The biological growth curve of the sample group was lower than that of the control group, indicating that it had an inhibitory effect on bacterial growth at this concentration and had an antibacterial effect. See the growth curve Figure 1 , Figure 2 .

[0072] 2. Experiments were carried out using Examples 1, 2, 3, 4 and 6, with the control group being phosphate buffered saline (PBS).

[0073] Table 4 Aerobic antibacterial test results

[0074]

[0075] As shown in Table 4, the antibacterial test results show that Example 1 has an antibacterial rate of more than 50% against Staphylococcus aureus and Escherichia coli, and has an antibacterial effect. Example 2 has an antibacterial rate of more than 50% against Staphylococcus aureus, Escherichia coli, and Candida albicans, and has an antibacterial effect. The antibacterial rates of Example 3, Example 4, and Example 6 against Candida albicans, Staphylococcus aureus, and Escherichia coli are all greater than 90%, and have a strong antibacterial effect.

[0076] Combination Figure 1 and Figure 2 As shown, the growth curve diagram shows that Example 1 and Example 2 have a certain inhibitory effect on the biological cell growth curve of Porphyromonas gingivalis and Streptococcus mutans. Example 3, Example 4, and Example 6 have a strong inhibitory effect on the biological cell growth curve of Porphyromonas gingivalis and Streptococcus mutans, and can reduce their reproduction rate and growth time.

Claims

1. A plant-based oral care product containing an Artemisia annua extract for clearing heat, protecting gums and inhibiting bacteria, characterized in that: The oral care product contains Artemisia annua extract, Zanthoxylum bungeana extract, Licorice extract, Isatis indigotica extract and Camellia chrysantha extract as active ingredients; wherein: the Artemisia annua extract is added in an amount of 0.005% to 5% of the total weight of the oral care product, the Zanthoxylum bungeana extract is added in an amount of 0.05% to 10% of the total weight of the oral care product, the Licorice extract is added in an amount of 0.01% to 5% of the total weight of the oral care product, the Isatis indigotica extract is added in an amount of 0.01% to 5% of the total weight of the oral care product, and the Camellia chrysantha extract is added in an amount of 0.01% to 5% of the total weight of the oral care product.

2. The plant fire-clearing, gum-protecting and antibacterial oral care product containing Artemisia annua extract according to claim 1, characterized in that: The weight ratio of the Artemisia annua extract, the Zygophyllum chinensis extract, the Licorice root extract, the Isatis indigotica extract and the Camellia chrysantha extract is 1:4-40:1-5:1-5:0.2-5.

3. The plant fire-clearing, gum-protecting and antibacterial oral care product containing Artemisia annua extract according to claim 1, characterized in that: The weight ratio of the Artemisia annua extract, the Zygophyllum chinensis extract, the Licorice root extract, the Isatis indigotica extract and the Camellia chrysantha extract is 1:4:1.5:1.5:1.5:

1.

4. The plant fire-clearing, gum-protecting and antibacterial oral care product containing Artemisia annua extract according to claim 1, characterized in that: The added amount of the Artemisia annua extract is 0.005% to 2% of the total weight of the oral care product.

5. The plant fire-clearing, gum-protecting and antibacterial oral care product containing Artemisia annua extract according to claim 1, characterized in that: The added amount of the Zanthoxylum bungeanum extract is 0.05% to 8% of the total weight of the oral care product.

6. The plant fire-clearing, gum-protecting and antibacterial oral care product containing Artemisia annua extract according to claim 1, characterized in that: The added amount of the licorice extract is 0.01% to 3% of the total weight of the oral care product.

7. The plant-based oral care product for clearing heat, protecting gums and inhibiting bacteria containing an Artemisia annua extract according to claim 1, characterized in that: The added amount of the isatis indigotica extract is 0.02% to 3% of the total weight of the oral care product.

8. The plant-based oral care product for clearing heat, protecting gums and inhibiting bacteria containing an Artemisia annua extract according to claim 1, characterized in that: The added amount of the Camellia chrysantha extract is 0.01% to 3% of the total weight of the oral care product.

9. The plant-based oral care product for clearing heat, protecting gums and inhibiting bacteria containing an Artemisia annua extract according to claim 1, characterized in that: The Artemisia annua extract is an ethanol extract; and / or the Zygophyllum chinensis extract is an ethanol extract; and / or the Licorice root extract is an ethanol extract; and / or the Isatis indigotica extract is an ethanol extract; and / or the Camellia chrysantha extract is an ethanol extract.

10. The plant fire-clearing, gum-protecting and antibacterial oral care product containing Artemisia annua extract according to claim 1, characterized in that: The oral care product is toothpaste, mouthwash, tooth powder, oral spray, oral rinse or chewing gum.

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