A blood-activating traditional Chinese medicine composition for relieving pain, a preparation method and application thereof

Abstract

The application belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to a blood-activating traditional Chinese medicine composition for relieving pain and a preparation method and application thereof. The blood-activating traditional Chinese medicine composition is composed of the following components in parts by weight: 10-20 parts of chuanxiong, 1-5 parts of aucklandia, 1-5 parts of dragon's blood, 15-30 parts of chizhou, 10-20 parts of licorice, 10-20 parts of myrrh, 5-15 parts of angelica, 10-15 parts of astragalus, 5-10 parts of eucommia and 10-15 parts of red peony root. The traditional Chinese medicine composition obtained by reasonable compounding has good effects of relieving pain and treating osteoarthritis.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of traditional Chinese medicine composition, and particularly relates to a blood-activating traditional Chinese medicine composition for relieving pain and a preparation method and application thereof. BACKGROUND

[0002] Tendon-bone joint pain and soft tissue and muscle injury, as well as periarthritis scapuli, hyperosteogeny, contusion and injury, chronic strain, lumbar intervertebral disc herniation and the like can all cause pain. These pains greatly affect people's daily life and cause inconvenience to people's daily life. The whole body muscle joint is also attacked by wind, cold, heat, damp, dry and fire "six evils", or external injury, chronic strain, and the "six evils" penetrate into the bone to cause "six poisons", which are commonly known as "bone poison". The "bone poison" exists on the bone and periosteum for a long time to form "residual poison". The residual poison causes the soft tissue around the bone and joint to repeatedly hyperemia, edema, adhesion and contracture, thereby leading to local blood circulation disorder and a large amount of acid substances stimulating blood vessels, damaging muscle tissue, and showing muscle tendon-bone joint pain, numbness, swelling, and even redness, swelling and deformation. The severe cases can cause destruction of joint bone, joint deformation, and loss of self-care ability. Tendon-bone pain is an important clinical manifestation of rheumatic bone disease.

[0003] Arthritis is a common autoimmune disease mainly manifested as chronic inflammation of joint tissue. The main manifestations of the affected joint are inflammatory cell infiltration, synovial membrane proliferation, pannus formation and the resulting damage to cartilage and bone. Arthritis belongs to the "arthralgia" category of traditional Chinese medicine. The "Huangdi's Canon of Internal Medicine" believes that the deficiency of qi and blood in the body, the invasion of wind, cold and dampness into the body, and the combination of the three evils, which flow into the tendons and blood vessels and block the joints, are the main causes and pathogenesis of arthralgia. Therefore, the evil of wind, cold and dampness is only the external condition for the occurrence of the disease, and the deficiency of qi and blood and the imbalance of the nutrient and defensive systems are the internal factors of the disease. The treatment of the disease should be to dispel wind and cold, remove dampness, tonify qi and blood, and unblock the collaterals. The occurrence of knee synovitis is mainly due to acute trauma of the knee joint, or sterile inflammation of the synovium caused by chronic strain, hyperosteogeny and rheumatic factors. The main pathological changes are as follows: first, the blood vessels of the synovium in the affected area are dilated and hyperemic, and plasma, red blood cells and white blood cells are exuded, producing a large amount of exudate; second, synovial cells are actively proliferated, and cell secretion is abnormally vigorous, producing a large amount of mucin, joint cavity effusion, joint pressure increase, hindering the circulation of the lymphatic system, and causing the accumulation of acidic metabolites in the joint, which can aggravate synovitis and make the disease worse in a vicious cycle. In the later stage, the synovium is thickened and adhesion, and the joint cartilage is atrophied, leading to knee joint dysfunction.

[0004] The drugs for treating orthopedic diseases in modern medicine are usually chemical drugs containing estrogen, which have great side effects in clinical use. Traditional Chinese medicines are favored by doctors and patients because of their unique efficacy and small side effects.

[0005] As disclosed in Chinese patent CN105168978A, a traditional Chinese medicine composition for treating muscle and joint pain by dispelling wind and cold, relaxing sinews and promoting blood circulation, removing blood stasis and resolving nodules is composed of the following components in parts by weight: honey 150-180 parts, beeswax 10-20 parts, cantharis 180-220 parts, soap needle 2-8 parts, dragon clothes 3-6 parts, frankincense 10-15 parts, myrrh 10-15 parts, cinnabar 5-10 parts, realgar 5-10 parts, earth yuan 10-15 parts, sea horse 10-15 parts, musk 1 part, Gastrodia 3-8 parts, Notopterygium 3-9 parts, Angelica pubescens 3-9 parts, cassia twig 4-8 parts, Sichuan rhizoma 4-8 parts, cistanche 5-10 parts. The traditional Chinese medicine composition and traditional Chinese medicine rehabilitation pill provided by the application adopt a plurality of traditional Chinese medicines with synergistic compatibility, are scientifically formulated, and have the effects of dispelling wind and cold, relaxing sinews and promoting blood circulation, removing blood stasis and resolving nodules, and are used for treating muscle and joint pain caused by wind, cold and dampness.

[0006] For example, as disclosed in Chinese patent CN103100017A, an external use traditional Chinese medicine health product for relieving muscle and joint pain is disclosed, and the weight parts of various raw materials in the traditional Chinese medicine health product are as follows: star anise 10-20 parts, Sichuan lovage 10-20 parts, salvia miltiorrhiza 10-20 parts, baizhi 10-20 parts, safflower 10-20 parts, kujay 10-20 parts, tendril grass 10-20 parts, shank 10-20 parts, giant knotweed 10-20 parts, silvertop 10-20 parts, asarum 10-20 parts, drynaria 10-20 parts, old crane grass 10-20 parts, white 10-20 parts, through bone grass 10-20 parts, myrrh 10-20 parts, frankincense 10-20 parts, rhizoma 10-20 parts, Notopterygium 10-20 parts, earthworm 10-20 parts. The application has certain effect on joints by using traditional Chinese medicines for tonifying kidney and strengthening bones, promoting blood circulation and removing blood stasis, can improve local arterial blood supply dysfunction, enhance local blood flow, promote bone trabecula regeneration, reduce cystic changes, harden bone absorption, repair cartilage site, and restore blood vessels and muscle atrophy.

[0007] However, the existing traditional Chinese medicine composition has many components, and the blood circulation effect is not obvious, which cannot meet the demand of relieving pain. SUMMARY

[0008] Based on the deficiencies in the prior art, the present application aims to provide a blood circulation traditional Chinese medicine composition for relieving pain, a preparation method and application thereof. In the implementation process, the present application controls various traditional Chinese medicine components reasonably, and the obtained traditional Chinese medicine composition has good effects of relieving pain and treating osteoarthritis through reasonable compatibility.

[0009] In order to achieve the above-mentioned purpose, the present application adopts the following technical scheme:

[0010] A blood-activating traditional Chinese medicine composition for relieving pain, which is composed of the following components in parts by weight: 10-20 parts of Chuanxiong Rhizoma, 1-5 parts of Huoxue, 1-5 parts of Xuejie, 15-30 parts of Niuxi, 10-20 parts of Gancao, 10-20 parts of Modyng, 5-15 parts of Danggui, 10-15 parts of Huangqi, 5-10 parts of Duzhong and 10-15 parts of Chishao.

[0011] Preferably, the blood-activating traditional Chinese medicine composition is composed of the following components in parts by weight: 12-18 parts of Chuanxiong Rhizoma, 2-3 parts of Huoxue, 2-3 parts of Xuejie, 20-28 parts of Niuxi, 8-15 parts of Gancao, 8-15 parts of Modyng, 8-12 parts of Danggui, 12-15 parts of Huangqi, 6-9 parts of Duzhong and 12-15 parts of Chishao.

[0012] More preferably, the blood-activating traditional Chinese medicine composition is composed of the following components in parts by weight: 14-16 parts of Chuanxiong Rhizoma, 2.5-3 parts of Huoxue, 2.5-3 parts of Xuejie, 22-26 parts of Niuxi, 7-8 parts of Gancao, 10-12 parts of Modyng, 8-12 parts of Danggui, 13-15 parts of Huangqi, 7-8 parts of Duzhong and 14-15 parts of Chishao.

[0013] As some preferred embodiments, the mass ratio of Chuanxiong Rhizoma, Modyng, Gancao and Duzhong is 1.5-2.5:1-2:1-2:1.

[0014] More preferably, the mass ratio of Chuanxiong Rhizoma, Modyng, Gancao and Duzhong is 2:2:1:1.

[0015] As some preferred embodiments, the mass ratio of Danggui, Niuxi, Huangqi and Chishao is 1:2-3:1-2:1-2.

[0016] More preferably, the mass ratio of Danggui, Niuxi, Huangqi and Chishao is 1:2.5:1.5:1.5.

[0017] As a preferred embodiment, the blood-activating traditional Chinese medicine composition is composed of the following components in parts by weight: 16 parts of Chuanxiong Rhizoma, 2.5 parts of Huoxue, 2.5 parts of Xuejie, 25 parts of Niuxi, 8 parts of Gancao, 12 parts of Modyng, 10 parts of Danggui, 15 parts of Huangqi, 8 parts of Duzhong and 15 parts of Chishao.

[0018] The application also provides a preparation method of the blood-activating traditional Chinese medicine composition, which comprises the following steps: taking the prescription amount of medicinal materials, adding water to decoct, filtering, obtaining decocting liquid, and concentrating the decocting liquid to thick extract to obtain the blood-activating traditional Chinese medicine composition.

[0019] As some preferred embodiments, the preparation method of the blood-activating traditional Chinese medicine composition comprises the following steps: taking the prescription amount of medicinal materials, adding water to decoct for 2-3 times, filtering, and concentrating the combined decocting liquid to relative density of 1.20-1.48 (60℃) to obtain the blood-activating traditional Chinese medicine composition.

[0020] As some preferred embodiments, the preparation method of the blood-activating traditional Chinese medicine composition comprises the following steps: taking formula amount of medicinal materials, adding 8-12 times of water to decoct for 2-3 hours, filtering to obtain decocting liquid 1, adding 6-10 times of water to decoct for 1-2 hours, filtering to obtain decocting liquid 2, and combining the decocting liquid 1 and the decocting liquid 2 and concentrating to a relative density of 1.20-1.48 (60 DEG C) thick extract to obtain the blood-activating traditional Chinese medicine composition.

[0021] The application further provides application of the blood-activating traditional Chinese medicine composition in preparation of a medicine for treating knee osteoarthritis.

[0022] The compatibility principle of the application is as follows:

[0023] The application takes Chuanqiong, E-zhu, Xuejie and Niuxi as monarch drugs, Ganzao, Muyang and Danggui as minister drugs, Huangqi as auxiliary drug and Duzhong and Chishao as ministerial drugs, and the obtained active traditional Chinese medicine composition has good effects of relieving pain and treating arthritis.

[0024] Ganzao, Muyang and Danggui are taken as minister drugs, Ganzao is sweet and neutral, and has no toxicity, can tonify the spleen and boost qi, clear heat and detoxify, dispel phlegm and relieve cough, and relieve pain, Muyang can activate blood and relieve pain, and dissipate swelling and promote tissue regeneration, and Danggui is sweet, pungent and warm, can tonify blood, activate blood, and regulate menstruation and relieve pain, and the three drugs are combined to enhance circulation of qi and blood, relieve pain, and assist the monarch drugs in tonifying qi and activating blood, and relieving pain.

[0025] Huangqi is taken as auxiliary drug, Huangqi is sweet and warm, and can tonify qi, consolidate the exterior, promote diuresis and remove toxin, discharge pus, and promote tissue regeneration, and can assist the monarch and minister drugs in relieving pain.

[0026] Duzhong and Chishao are taken as ministerial drugs, Duzhong is sweet and warm, can tonify the liver and kidney, strengthen the muscles and bones, regulate the chong and ren, and consolidate the channels and prevent miscarriage, Chishao is sweet and cold, and can clear heat and cool blood, and dissipate blood stasis, and the two drugs are combined to regulate the other drugs, ensure the tonifying effect to be mild and durable, and avoid discomfort caused by excessive heat.

[0027] Compared with the prior art, the application has the following beneficial effects:

[0028] (1) The application takes Chuanqiong, E-zhu, Xuejie and Niuxi as monarch drugs, Ganzao, Muyang and Danggui as minister drugs, Huangqi as auxiliary drug, and Duzhong and Chishao as ministerial drugs, and the obtained active traditional Chinese medicine composition has good effects of relieving pain and treating arthritis.

[0029] (2) The application improves the efficacy of relieving pain and treating arthritis of the blood-activating traditional Chinese medicine composition by controlling the mass ratio of Chuanxiong, Myrrha, Licorice and Eucommia as 1.5-2.5:1-2:1-2:1; and the mass ratio of Angelica, Radix Cyathulae, Radix Astragali and Radix Paeoniae Rubra as 1:2-3:1-2:1-2. DETAILED DESCRIPTION

[0030] Various exemplary embodiments of the present application will now be described in detail, which should be considered in a descriptive sense only and not for purposes of limitation to the application described herein. It will be appreciated that the detailed description is only exemplary and is not intended to limit the application described herein.

[0031] It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. Additionally, the use of the term "about" in relation to a value is intended to encompass the value ±10% of the stated value. Unless otherwise specified, all ranges of values are to be understood as encompassing each and every sub-range of values within the indicated ranges. These sub-ranges of values are inherently described. The use of the term "at least one" in relation to a value is intended to encompass the value and any sub-range of values within the indicated range. These sub-ranges of values are inherently described. The use of the term "one or more" in relation to a value is intended to encompass the value and any sub-range of values within the indicated range. These sub-ranges of values are inherently described.

[0032] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present application, the preferred methods and materials are described. It will be apparent to those skilled in the art that many modifications, both to methods and materials, can be practiced without departing from the scope of the application. Other embodiments of the application will be apparent to those skilled in the art from consideration of the specification and practice of the application disclosed herein. Various embodiments of the application are presented in reference to the specification, drawings and examples. It is to be understood that features of the various embodiments can be combined, substituted, or removed for purposes of constructing other embodiments of the application.

[0033] As used herein, the terms "comprises", "comprising", "includes", "including", "has", "having" or variants thereof are open-ended, and specifically include a complete range of equivalence and / or one or more elements or articles without necessarily being limited to only those elements or articles. Unless otherwise specified, the use of the singular includes the plural.

[0034] Example 1

[0035] A blood-activating traditional Chinese medicine composition for relieving pain, which is composed of the following components in parts by weight: Chuanxiong 10 parts; E Zhu 1 part; Dragon's Blood 1 part; Radix Cyathulae 15 parts; Licorice 10 parts; Myrrha 10 parts; Angelica 5 parts; Radix Astragali 10 parts; Eucommia 5 parts and Radix Paeoniae Rubra 10 parts.

[0036] The preparation method of the blood-activating traditional Chinese medicine composition is as follows: take the formula amount of medicinal materials, add 8 times of water and decoct for 2 hours, filter to obtain decoction liquid 1, add 6 times of water and decoct for 1 hour, filter to obtain decoction liquid 2, combine the decoction liquid 1 and the decoction liquid 2, and concentrate to a relative density of 1.3 (60℃) thick extract paste to obtain the blood-activating traditional Chinese medicine composition.

[0037] Example 2

[0038] A blood-activating traditional Chinese medicine composition for relieving pain, consisting of the following components in parts by weight: Chuanxiong 20 parts; E Zhu 5 parts; Dragon's Blood 5 parts; Huikui 30 parts; Gancao 20 parts; Mody 20 parts; Danggui 15 parts; Huangqi 15 parts; Duzhong 10 parts; and Chishao 15 parts.

[0039] The preparation method of the blood-activating traditional Chinese medicine composition is as follows: take the formula amount of medicinal materials, add 12 times water and decoct for 3 h, filter to obtain decoction liquid 1, then add 10 times water and decoct for 2 h, filter to obtain decoction liquid 2, combine decoction liquid 1 and decoction liquid 2, and concentrate to a thick extract with a relative density of 1.3 (60°C) to obtain the blood-activating traditional Chinese medicine composition.

[0040] Example 3

[0041] A blood-activating traditional Chinese medicine composition for relieving pain, consisting of the following components in parts by weight: Chuanxiong 16 parts; E Zhu 2.5 parts; Dragon's Blood 2.5 parts; Huikui 25 parts; Gancao 8 parts; Mody 12 parts; Danggui 10 parts; Huangqi 15 parts; Duzhong 8 parts; and Chishao 15 parts.

[0042] The preparation method of the blood-activating traditional Chinese medicine composition is as follows: take the formula amount of medicinal materials, add 10 times water and decoct for 2.5 h, filter to obtain decoction liquid 1, then add 8 times water and decoct for 1.5 h, filter to obtain decoction liquid 2, combine decoction liquid 1 and decoction liquid 2, and concentrate to a thick extract with a relative density of 1.3 (60°C) to obtain the blood-activating traditional Chinese medicine composition.

[0043] Comparative Example 1

[0044] The only difference between Comparative Example 1 and Example 3 is that Huikui is replaced by Huangqi of the same weight fraction, and Dragon's Blood is replaced by Olibanum of the same weight fraction; the rest are the same.

[0045] Comparative Example 2

[0046] The only difference between Comparative Example 2 and Example 3 is that E Zhu is replaced by Honghua of the same weight fraction, and Huikui is replaced by Taoren of the same weight fraction; the rest are the same.

[0047] Comparative Example 3

[0048] The only difference between Comparative Example 3 and Example 3 is that Danggui is replaced by Baizhi of the same weight fraction, and Gancao is replaced by Jixuecao of the same weight fraction; the rest are the same.

[0049] Comparative Example 4

[0050] The only difference between Comparative Example 4 and Example 3 is that Danggui is replaced by Sanqi of the same weight fraction; the rest are the same.

[0051] Comparative Example 5

[0052] The only difference between the present example and Example 3 is that the blood-activating traditional Chinese medicine composition comprises, by weight fraction, the following components: Chuanqiong 16 parts; E Zhu 2.5 parts; Dried Dragon's Blood 2.5 parts; Huangqi 25 parts; Gancao 8 parts; Mody 12 parts; Danggui 10 parts; Niuqi 15 parts; Zhuduzhong 8 parts; and Chishao 15 parts; and the rest is the same as in Example 3.

[0053] Comparative Example 6

[0054] The only difference between the present example and Example 3 is that the blood-activating traditional Chinese medicine composition comprises, by weight fraction, the following components: Chuanqiong 22 parts; E Zhu 2.5 parts; Dried Dragon's Blood 2.5 parts; Niuqi 25 parts; Gancao 4 parts; Mody 9 parts; Danggui 10 parts; Huangqi 15 parts; Zhuduzhong 15 parts; and Chishao 15 parts; and the rest is the same as in Example 3.

[0055] Comparative Example 7

[0056] The only difference between the present example and Example 3 is that the blood-activating traditional Chinese medicine composition comprises, by weight fraction, the following components: Chuanqiong 16 parts; E Zhu 2.5 parts; Dried Dragon's Blood 2.5 parts; Niuqi 20 parts; Gancao 8 parts; Mody 12 parts; Danggui 6 parts; Huangqi 20 parts; Zhuduzhong 8 parts; and Chishao 19 parts; and the rest is the same as in Example 3.

[0057] Pain-relieving effect of Experimental Example 1

[0058] 1. Experimental animals: 130 SPF male rats, 6-8 weeks old, weighing 180-220 g.

[0059] 2. Model establishment: The rats were anesthetized with urethane, and after losing consciousness, the right knee joint was prepared for skinning, then iodine was used for disinfection, and 3 mg / 50 μL of sodium iodoacetate was injected into the knee joint cavity of the rats.

[0060] 3. Grouping: The rats were randomly divided into 12 groups: a model control group, Experimental Example 1, Experimental Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, Comparative Example 5, Comparative Example 6, Comparative Example 7, and a Shentongzhuyu Decoction group; 10 rats in each group; another 10 rats were taken as a normal control group and injected with the same volume of normal saline.

[0061] Shentongzhuyu Decoction group: 3 g of Qianjie, 6 g of Chuanqiong, 9 g of Taoren, 9 g of Honghua, 6 g of Gancao, 3 g of Qianghuo, 6 g of Mody, 9 g of Danggui, 6 g of Lingzhi (fried), 3 g of Xiangfu, 9 g of Niuqi, and 6 g of Dilong (soil removed) were added to 10 times the amount of water and decocted for 2.5 h, filtered to obtain decoction 1, and then 8 times the amount of water was added and decocted for 2 h, filtered to obtain decoction 2, and the decoction 1 and decoction 2 were combined and concentrated to a thick extract to obtain the Shentongzhuyu Decoction.

[0062] 4. Administration: 3 mg / 50 μL MIA was injected on the 10th day, and the Shengyan Zhuyu Decoction (prepared into a liquid in advance with normal saline) was administered orally at a dose of 18 g / kg, with a volume of 10 mL / kg. The normal control group and the model control group were administered with the same amount of normal saline, the Shentong Zhuyu Decoction was administered at a dose of 18 g / kg, with a volume of 10 mL / kg, once a day, for 14 consecutive days.

[0063] 5. Measurement of mechanical allodynia threshold: After the administration, the right hind paw of the rat was stimulated with a standard nylon wire, and the label of the nylon wire was gradually increased in order from the thinnest to the thickest, until the right hind leg of the rat was lifted, and the label of the nylon wire used was the mechanical pain threshold, which was measured in grams (g).

[0064] 6. Measurement of thermal pain threshold: After the administration, the right hind paw of the rat was irradiated with a laser heat source lamp to measure the thermal pain threshold, the intensity of the laser heat source was 20%, and the irradiation time when the right hind leg of the rat was lifted was the thermal pain threshold, which was measured in seconds (s).

[0065] 7. The data were analyzed and processed by SPSS21.0 statistical software, and the comparison between groups was performed by t-test.

[0066] 8. Result analysis

[0067] 8.1 Mechanical allodynia threshold: as shown in Table 1:

[0068] Table 1

[0069] Group Before administration (g) After administration (g) Normal control group 21.3±0.52 20.8±0.65 Model control group 10 ± 1.64 ** ]] 10.3 ± 0.31 ** ]] Example 1 10.3±1.22 14.7 ± 0.52 ## ]] Example 2 10.5±1.34 14.3 ± 1.26 # ]] Example 3 11.2±0.92 14.8 ± 1.24 ## ]] Comparative example 1 10.6±1.16 12.3±1.20 Comparative example 2 10.8±1.25 13±1.68 Comparative example 3 10.3±1.22 13.3±1.92 Comparative example 4 11±1.31 13.7 ± 1.26 # <!-- 5 -->]]> Comparative example 5 10.5±0.96 11.7±1.75 Comparative example 6 10.1±1.85 12.6±0.86 Comparative example 7 10.6±1.34 12.1±2.17 Shentong Zhuyu decoction group 10.5±1.53 14.1 ± 1.43 # ]]

[0070] Note: compared with the normal control group, ** P<0.01; compared with the model control group, ## P<0.01, # P<0.05.

[0071] 8.2 Thermal pain threshold: as shown in Table 2:

[0072] Table 2

[0073]

[0074]

[0075] Note: compared with the normal control group, ** P<0.01; compared with the model control group, ## P<0.01, # P<0.05.

[0076] As can be seen from Table 1 and Table 2, the blood-activating traditional Chinese medicine composition of the embodiments 1-3 has significant differences in mechanical allodynia threshold and thermal pain threshold compared with the model group; the mechanical allodynia threshold and thermal pain threshold are greater compared with the Shentong Zhuyu decoction group, indicating that the blood-activating traditional Chinese medicine composition of the embodiments 1-3 has better analgesic effect compared with the Shentong Zhuyu decoction.

[0077] In the comparative example 1, the Radix Cyathulae is replaced by Astragalus membranaceus in the same weight fraction, and the blood stasis is replaced by Boswellia sacra in the same weight fraction; in the comparative example 2, the Curcuma zedoaria is replaced by Carthamus tinctorius in the same weight fraction, and the Radix Cyathulae is replaced by Semen Persicae in the same weight fraction; in the comparative example 3, the Angelica sinensis is replaced by Radix Angelicae Pubescentis in the same weight fraction, and the Glycyrrhiza uralensis is replaced by Elephantopus japonicus in the same weight fraction; the blood-activating traditional Chinese medicine composition obtained has no significant difference in mechanical allodynia threshold and thermal pain threshold compared with the model group;

[0078] In the comparative example 4, the Angelica sinensis is replaced by Panax notoginseng in the same weight fraction; the blood-activating traditional Chinese medicine composition obtained has significant differences in mechanical allodynia threshold and thermal pain threshold compared with the model group, but the effect is not as good as that of the embodiments 1-3.

[0079] In the comparative example 5, the Astragalus membranaceus and the Radix Cyathulae are replaced by each other in the weight fraction; the blood-activating traditional Chinese medicine composition obtained has no significant difference in mechanical allodynia threshold and thermal pain threshold compared with the model group.

[0080] In the comparative example 6, the mass ratio of Chuanxiong Rhizoma, Myrrha, Glycyrrhiza uralensis and Eucommia ulmoides is not in the range of 1.5-2.5:1-2:1-2:1; in the comparative example 7, the mass ratio of Angelica sinensis, Radix Cyathulae, Astragalus membranaceus and Radix Paeoniae Rubra is not in the range of 1:2-3:1-2:1-2; the blood-activating traditional Chinese medicine composition obtained has no significant difference in mechanical allodynia threshold and thermal pain threshold compared with the model group.

[0081] Experimental example 2: effect of treating arthritis

[0082] 1. Experimental animals: 130 SPF male SD rats, 6-8 weeks old, weighing 180-220 g.

[0083] 2. Modeling: take the rats, inhale isoflurane for about 5 s to achieve the effect of general anesthesia, then use a sterile insulin syringe to suck 50 mg·mL-1 of sodium urate suspension 25 μL, inject it into the right ankle joint of the rat in a 30° direction, and the opposite side of the joint cavity swells to indicate successful modeling. -1

[0084] 3. Grouping: the successfully modeled rats are randomly divided into 12 groups: a model control group, embodiments 1, 2 and 3, comparative examples 1, 2, 3, 4, 5, 6 and 7, and a Shentong Zhuyu decoction group; 10 rats in each group; another 10 rats are taken as a normal control group and injected with the same volume of normal saline. ​

[0085] 4. Administration: the examples and comparative examples are respectively administered with the corresponding blood-activating traditional Chinese medicine composition (previously prepared into liquid with normal saline) 18 g / kg, the administration volume is 10 mL / kg, the normal control group and the model control group are administered with the same amount of normal saline, the Shitong Zhuyu decoction group is administered with the dose of 18 g / kg, the administration volume is 10 mL / kg, once a day, for 14 days.

[0086] 5. Detection: after the administration is completed, the rats are sacrificed by the cervical vertebra dislocation method, the model rats are surgically opened, the knee joint fluid of the rats is collected, and the IL-1β, IL-6 and TNF-α levels in the knee joint fluid of the rats are determined by the ELISA method.

[0087] 6. The SPSS21.0 statistical software is used for analysis and processing, the data are represented by (x±s), and the t test is used for comparison between groups.

[0088] 7. The results are shown in Table 3:

[0089] Table 3

[0090] Group IL-1β (pg / mL) IL-6 (pg / mL) TNF-α (pg / mL) Normal control group 134.7±13.19 40.2±7.72 75.6±8.62 Model control group 258.6 ± 9.97 ** ]] 203.6 ± 8.38 ** ]] 198.5 ± 25.31 ** ]] Example 1 161.5 ± 8.22 ## ]] 71 ± 7.79 ## ]] 93.2 ± 6.82 ## ]] Example 2 164 ± 11.77 ## ]] 71.2 ± 10.19 ## ]] 93.9 ± 8.52 ## ]] Example 3 160.2 ± 8.95 ## ]] 70.3 ± 7.29 ## ]] 92.5 ± 7.38 ## ]] Comparative example 1 233.3±12.47 161.7±23.21 164±17.55 Comparative example 2 230.7±13.02 154±25.67 160.5±22.81 Comparative example 3 230±16.32 152.5±26.55 156.8±24.94 Comparative example 4 215.8 ± 19.43 # ]] 121.6 ± 26.25 # ]] 126.3 ± 21.61 # ]] Comparative example 5 226.2±32.99 162.5±27.15 168.1±25.21 Comparative example 6 237.5±34.9 175.6±28.12 181.3±27.68 Comparative example 7 235.3±30.86 175.1±27.95 179.5±25.95 Shentong Zhuyu decoction group 216.8 ± 16.36 # ]] 127.4 ± 22.45 # ]] 128.3 ± 23.01 # ]]

[0091] Note: compared with the normal control group, ** P<0.01; compared with the model control group, ## P<0.01, # P<0.05.

[0092] As can be seen from Table 3, the blood-activating traditional Chinese medicine composition of the examples 1-3 has significant differences in IL-1β, IL-6 and TNF-α compared with the model group; compared with the Shitong Zhuyu decoction group, IL-1β, IL-6 and TNF-α are smaller, which indicates that the blood-activating traditional Chinese medicine composition of the examples 1-3 has a better effect on treating arthritis than the Shitong Zhuyu decoction.

[0093] In the comparative example 1, the radix cyathulae is replaced with the same weight fraction of radix astragali, and the blood is replaced with the same weight fraction of olibanum; in the comparative example 2, the curcuma zedoaria is replaced with the same weight fraction of safflower, and the radix cyathulae is replaced with the same weight fraction of persicae semen; in the comparative example 3, the angelica sinensis is replaced with the same weight fraction of radix anemarrhenae, and the radix glycyrrhizae is replaced with the same weight fraction of radix rubi; the blood-activating traditional Chinese medicine composition obtained has no significant difference in IL-1β, IL-6 and TNF-α compared with the model group;

[0094] In the comparative example 4, the angelica sinensis is replaced with the same weight fraction of notoginseng, and the blood-activating traditional Chinese medicine composition obtained has significant differences in IL-1β, IL-6 and TNF-α compared with the model group, but the effect is not as good as that of the examples 1-3.

[0095] Comparative Example 5 replaces the weight proportions of Huangqi and Niuxi with each other, and the obtained blood-activating traditional Chinese medicine composition has no significant difference in IL-1β, IL-6 and TNF-α compared with the model group;

[0096] Comparative Example 6 has a mass ratio of Chuanxiong, Mo Yao, Gancao and Duzhong not in the range of 1.5-2.5:1-2:1-2:1; Comparative Example 7 has a mass ratio of Dangui, Niuxi, Huangqi and Chishao not in the range of 1:2-3:1-2:1-2; and the obtained blood-activating traditional Chinese medicine composition has no significant difference in IL-1β, IL-6 and TNF-α compared with the model group.

[0097] The above examples are only used to help understand the method of the present application and its core idea. It should be noted that for those skilled in the art, without departing from the principles of the present application, the present application can be improved and modified in several ways, and these improvements and modifications also fall within the scope of the claims of the present application.

Claims

1. A pain-relieving and blood-activating Chinese medicinal composition, characterized in that: The invention is composed of the following components by weight: 10-20 parts of Chuanxiong; 1-5 parts of Curcuma; 1-5 parts of Sanguisorba officinalis; 15-30 parts of Achyranthes bidentata; 10-20 parts of Licorice; 10-20 parts of Myrrh; 5-15 parts of Angelica sinensis; 10-15 parts of Astragalus; 5-10 parts of Eucommia ulmoides and 10-15 parts of Red Peony Root.

2. A pain-relieving and blood-activating Chinese medicinal composition, characterized in that: The blood-activating Chinese medicinal composition is composed of the following components by weight: 12-18 parts of Chuanxiong; 2-3 parts of Curcuma; 2-3 parts of Sanguisorba officinalis; 20-28 parts of Achyranthes bidentata; 8-15 parts of Licorice; 8-15 parts of Myrrh; 8-12 parts of Angelica sinensis; 12-15 parts of Astragalus; 6-9 parts of Eucommia ulmoides and 12-15 parts of Red Peony Root.

3. A pain-relieving and blood-activating Chinese medicinal composition, characterized in that: The blood-activating Chinese medicinal composition is composed of the following components by weight: 14-16 parts of Chuanxiong; 2.5-3 parts of Curcuma; 2.5-3 parts of Sanguisorba officinalis; 22-26 parts of Achyranthes bidentata; 7-8 parts of Licorice; 10-12 parts of Myrrh; 8-12 parts of Angelica sinensis; 13-15 parts of Astragalus; 7-8 parts of Eucommia and 14-15 parts of Red Peony Root.

4. The blood-activating Chinese medicinal composition according to claim 3, characterized in that: The mass ratio of the chuanxiong rhizome, myrrh, liquorice and eucommia bark is 1.5-2.5:1-2:1-2:

1.

5. The blood-activating Chinese medicinal composition according to claim 3, characterized in that: The mass ratio of the angelica sinensis, Achyranthes bidentata, Astragalus membranaceus and Paeonia lactiflora is 1:2-3:1-2:1-2.

6. The blood-activating Chinese medicinal composition according to claim 5, characterized in that: The mass ratio of the angelica sinensis, Achyranthes bidentata, Astragalus membranaceus and Paeonia lactiflora is 1:2.5:1.5:1.

5.

7. The blood-activating Chinese medicinal composition according to claim 6, characterized in that: The blood-activating Chinese medicinal composition is composed of the following components by weight: 16 parts of Chuanxiong; 2.5 parts of Curcuma; 2.5 parts of Sanguisorba officinalis; 25 parts of Achyranthes bidentata; 8 parts of Licorice; 12 parts of Myrrh; 10 parts of Angelica sinensis; 15 parts of Astragalus; 8 parts of Eucommia ulmoides and 15 parts of Red Peony Root.

8. The method for preparing the blood-activating Chinese medicinal composition according to any one of claims 1 to 7, characterized in that: The method comprises the following steps: taking a prescribed amount of medicinal materials, adding water to boil, filtering to obtain a decoction, and concentrating the decoction to a thick extract to obtain a blood-activating Chinese medicine composition.

9. Use of the blood-activating Chinese medicinal composition according to any one of claims 1 to 7 in the preparation of a medicament for treating knee osteoarthritis.

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