Equipment and method for extracting flavone and anthocyanin from waxberry kernels

Abstract

The invention relates to the technical field of waxberry fruit extraction, and discloses a waxberry kernel brass and anthocyanin extraction device and method, fresh waxberry fruits are prepared, the fresh waxberry fruits are washed to remove surface dirt, the whole fruits are pulped, the kernels are subjected to low temperature freeze drying treatment, and the dried kernels are obtained; and filling the dried kernels into a supercritical fluid extraction device, setting pressure and temperature, and adding a cosolvent ethanol to obtain an extract of flavone and anthocyanin compounds. According to the equipment and method for extracting brass and anthocyanin from waxberry kernels, fresh waxberry fruits are selected and washed with a citric acid aqueous solution with the pH value of 3.5, so that surface dirt is removed, meanwhile, microbial growth is inhibited, and the quality and sanitary conditions of raw materials are ensured; dried kernels are put into a supercritical fluid extraction device, accurate pressure and temperature are set, and a proper amount of ethanol is added through an automatic liquid adding system to serve as a cosolvent.

Description

Technical Field

[0001] The invention relates to the technical field of bayberry fruit extraction, in particular to an extraction device and method for bayberry fruit core brass and anthocyanin. Background Art

[0002] Bayberry pit extraction refers to the process of separating and obtaining valuable chemical components from the hard seed part inside the bayberry fruit (i.e., the pit). These components include but are not limited to flavonoids and anthocyanins, which have multiple health benefits such as antioxidant and anti-inflammatory effects, and have broad application prospects in the fields of food additives, health products, and drug development. However, since the pit has a compact structure and is rich in difficult-to-handle substances such as cellulose, specially designed methods and techniques are required to effectively extract and purify these active ingredients. The present invention provides an innovative method for extracting flavonoids and anthocyanins from bayberry pits, which aims to overcome many challenges in the prior art and achieve an efficient, environmentally friendly, and high-quality extraction process.

[0003] In the prior art, the pretreatment of bayberry kernels is often not fine enough, resulting in a large number of impurities, which affects the quality of the subsequent extraction process. In addition, the traditional method cannot efficiently extract the target compound during the extraction process and is prone to introduce impurities. At the same time, the purification steps in the prior art are usually cumbersome, making it difficult to effectively remove impurities and obtain a high-purity target product. Due to the lack of effective stabilization treatment, traditional extracts are easily affected by factors such as oxidation, which reduces their shelf life and biological activity. Summary of the invention

[0004] The purpose of this section is to summarize some aspects of embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and the invention title of this application to avoid blurring the purpose of this section, the specification abstract and the invention title, and such simplifications or omissions cannot be used to limit the scope of the present invention.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A method for extracting bayberry fruit core brass and anthocyanin, comprising:

[0007] Prepare fresh bayberry fruits, wash them to remove surface dirt, peel them and take out the cores, and perform low-temperature freeze-drying on the cores to obtain dried cores;

[0008] The dried fruit core is placed in a supercritical fluid extraction device, the pressure and temperature are set, and the co-solvent ethanol is added to obtain an extract of flavonoids and anthocyanidin compounds;

[0009] The extract of flavonoids and anthocyanidins is centrifuged to remove large particle impurities, and the solvent is evaporated using a rotary evaporator to obtain a crude extract solution;

[0010] The crude extract solution is selectively adsorbed by molecularly imprinted polymer to obtain the purified target product;

[0011] The purified target product is stabilized by using an antioxidant from natural sources to obtain flavonoid and anthocyanidin extracts.

[0012] As a further solution of the present invention: the fresh bayberry fruit is prepared, cleaned to remove surface dirt, peeled and pitted, and the pit is subjected to low-temperature freeze-drying treatment to obtain a dried pit, and the specific steps are:

[0013] Select fresh bayberry fruits, use a citric acid solution with a pH value of 3.5 and 5% salt, and wash the bayberry fruits at room temperature to remove dirt, dust and insects on the surface;

[0014] The centrifuge is used to separate the bayberry and the pit. Under the centrifugal force generated by high-speed rotation, the heavier pit will settle at the bottom of the centrifuge tube, while the lighter pulp will remain on the upper layer.

[0015] The fruit core is placed in an ultra-low temperature refrigerator at -80℃ for rapid freezing, then transferred to a freeze dryer and gradually heated to room temperature under vacuum conditions. The entire drying process is maintained between -40℃ and -50℃, with the relative pressure set below 0.1mbar, until the moisture content is reduced to 5%, and the dried fruit core is obtained.

[0016] As a further solution of the present invention: the dried fruit core is placed in a supercritical fluid extraction device, the pressure and temperature are set, and the co-solvent ethanol is added to obtain an extract of flavonoids and anthocyanidin compounds, and the specific steps are:

[0017] The dried fruit core is placed into an extraction kettle of a supercritical fluid extraction device;

[0018] The supercritical fluid extraction device was set to an extraction pressure of 350 bar and an extraction temperature of 40 °C;

[0019] Add ethanol as a co-solvent to the device at a ratio not exceeding 5% by volume;

[0020] Turn on the supercritical fluid extraction device, start the extraction program, and set the extraction time to 60 minutes;

[0021] When the extraction is completed, a supercritical fluid extract of flavonoids and anthocyanidin compounds is obtained from the collection container;

[0022] The extraction kinetics-based optimization of supercritical fluid extraction process is introduced, and the expression is:

[0023]

[0024] Where e is the extraction efficiency, P is the extraction pressure, V co2 is the volume flow rate of supercritical carbon dioxide, R is the ideal gas constant, T is the absolute temperature, k eth anol is the ethanol enhancement factor, C target is the concentration of the target compound, and A is the mass transfer area.

[0025] As a further solution of the present invention: the extract of flavonoids and anthocyanin compounds is subjected to a centrifuge to remove large particle impurities, and a rotary evaporator is used to evaporate the solvent to obtain a crude extract solution, and the specific steps are as follows:

[0026] transferring the supercritical fluid extract of flavonoid and anthocyanin compounds into a container dedicated to the centrifuge;

[0027] Select Eppendorf 5810R high-speed refrigerated centrifuge, set the speed to 3500 rpm, and maintain the temperature at 4°C;

[0028] The centrifugation time was set to 20 minutes to allow the suspended large particles of impurities to settle to the bottom of the container to form a precipitate, and a clarified extract was obtained;

[0029] Transfer the clarified extract to the evaporating flask of the rotary evaporator R-215, set the water bath temperature to 40°C, set the system vacuum to about 200 mbar, and set the rotation speed to 120 rpm;

[0030] Start the rotary evaporator and begin the evaporation process, and continue to monitor the evaporation progress until most of the solvent is removed and a small amount of concentrate remains;

[0031] When an appropriate amount of concentrated liquid is observed to remain in the evaporating flask, stop heating and release the vacuum state to restore the system to normal pressure;

[0032] The concentrated crude extract solution is taken out from the evaporating flask, transferred to a clean storage container, and a crude extract solution is obtained.

[0033] As a further solution of the present invention: the introduction of physical and chemical principles based on the optimization of centrifugal separation and rotary evaporation process, the expression is:

[0034]

[0035] Among them, C final is the concentration of the final crude extract solution, C initial is the concentration of the initial extract, V initial is the initial volume, Vfinal is the final volume, k is the evaporation rate constant, t is the evaporation time, F centrifuge is the centrifugal force, M impurities is the impurity mass, A filter is the effective filtration area.

[0036] As a further solution of the present invention: the molecular imprinting polymer is used to selectively adsorb the crude extract solution to obtain the purified target product, and the specific steps are:

[0037] According to the chemical structure characteristics of flavonoids and anthocyanins, molecular imprinting polymers that specifically recognize compounds are designed and synthesized;

[0038] MIPs were prepared by thermal polymerization under anhydrous conditions using template molecules quercetin as a representative of flavonoids, cyanidin as a representative of anthocyanins, functional monomer methacrylic acid, crosslinker ethylene glycol dimethacrylate and initiator;

[0039] The synthesized MIPs are filled into a solid phase extraction column;

[0040] The concentrated crude extract solution was slowly passed through the solid phase extraction column containing MIPs to allow the target flavonoids and anthocyanins in the solution to fully contact with the MIPs, and the flow rate was controlled at 0.5 mL / min;

[0041] Use low-polarity solvent water as eluent to remove non-target substances that are not adsorbed;

[0042] After confirming that the non-target substances have been completely eluted, switch to a high-polarity eluent, a methanol / water mixture, with a set ratio of 7:3;

[0043] Collect the desorbed liquid to obtain the purified target product.

[0044] As a further solution of the present invention: the purified target product is stabilized by using a natural antioxidant to obtain flavonoid and anthocyanidin extracts, and the specific steps are as follows:

[0045] Selecting a natural antioxidant vitamin C, and preparing it into a 5% w aqueous solution according to the expected final product concentration;

[0046] Add the high-purity flavonoids and anthocyanins target products collected after desorption to the above antioxidant solution, adding 1-2 mL of antioxidant solution per 100 mL of target product;

[0047] Allow the mixed solution to stand at room temperature for 30 minutes to 1 hour to allow the antioxidant to form a complex with the target compound;

[0048] After stabilization, the mixed solution was filtered using a 0.45 μm filter membrane to remove precipitates or macromolecular impurities.

[0049] Use gentle stirring or ultrasonic-assisted methods to fully mix the two, and avoid loss or destruction of ingredients due to violent stirring;

[0050] The stabilized and filtered flavonoid and anthocyanin extracts were packaged into dark glass bottles and stored in a cool, dry, dark place at a temperature of 25°C and a humidity of 60%.

[0051] An extraction device for bayberry fruit core brass and anthocyanin, comprising: a pretreatment and low-temperature freeze-drying module, a supercritical fluid extraction module, a preliminary filtration and concentration module, a molecular imprinting polymer adsorption module, a stabilization treatment module, and a packaging and storage module;

[0052] The pretreatment and low-temperature freeze-drying module is located at the front end of the extraction equipment and is connected to the cleaning device through a conveyor belt, and is used to select and clean fresh bayberry fruits, peel them and remove the cores;

[0053] The pretreatment and low-temperature freeze-drying module comprises an ultra-low temperature refrigerator and a freeze dryer, which are connected by a pipeline and are used for rapid freezing and gradually heating under vacuum conditions to remove moisture, and finally obtain a dried fruit core;

[0054] The supercritical fluid extraction module is immediately after the pretreatment and low-temperature freeze-drying module and is physically connected via an automated feeding system to feed the dried fruit cores into the extraction kettle;

[0055] The supercritical fluid extraction module is equipped with a pressure regulating valve and a temperature controller, which can accurately set the extraction conditions of 350 bar pressure and 40 ° C temperature, and add an appropriate amount of ethanol as a co-solvent through the automatic liquid addition system to complete the efficient extraction of the target compound;

[0056] The preliminary filtration and concentration module is located after the SFE module, connected to the SFE module through a pumping system, and receives the extract flowing out of the SFE module;

[0057] The preliminary filtration and concentration module is equipped with a centrifuge and a rotary evaporator, the centrifuge is used to remove large particle impurities, and the rotary evaporator is responsible for evaporating the solvent under controlled conditions to obtain a crude extract solution;

[0058] The molecularly imprinted polymer adsorption module is located after the preliminary filtration and concentration module, and is connected to the former through a precision metering pump to receive the crude extract solution that has undergone preliminary treatment;

[0059] The molecularly imprinted polymer adsorption module has a built-in special solid phase extraction column filled with MIPs designed and synthesized for flavonoids and anthocyanins. The target compound is highly selectively adsorbed by slow flow, and then desorbed by using eluents of different polarities to collect the purified target product.

[0060] The stabilization treatment module is located after the MIPs adsorption module and is connected through a liquid transmission pipeline to receive the high-purity target product desorbed;

[0061] The stabilization treatment module is equipped with a mixing and stirring device and a constant temperature static zone, which can add natural antioxidants according to a predetermined ratio and ensure that the two are fully mixed and uniformly formed by gentle stirring or ultrasonic-assisted methods to form a stable complex;

[0062] The packaging and storage module is located after the stabilization treatment module and is connected via a sealed conveyor belt to receive the final product after stabilization treatment;

[0063] The stabilization processing module has an automatic packaging function, which can accurately distribute the product into dark glass bottles or other light-proof containers and store them in sealed containers.

[0064] The present invention provides a computer device, comprising a memory and a processor, wherein the memory stores a computer program, wherein: when the computer program is executed by the processor, any step of the method for extracting bayberry fruit core brass and anthocyanins as described in the first aspect of the present invention is implemented.

[0065] The present invention provides a computer-readable storage medium having a computer program stored thereon, wherein: when the computer program is executed by a processor, any step of the method for extracting bayberry fruit core brass and anthocyanins as described in the first aspect of the present invention is implemented.

[0066] Compared with the prior art, the present invention has the following beneficial effects:

[0067] By selecting fresh bayberry fruits and washing them with a citric acid aqueous solution with a pH value of 3.5 and salt, we can remove surface dirt and pests such as insects, thereby ensuring the quality and sanitary conditions of the raw materials. By loading the dried fruit cores into a supercritical fluid extraction device, setting precise pressure and temperature, and adding an appropriate amount of ethanol as a co-solvent through an automatic liquid addition system, we can achieve efficient extraction of the target compounds, significantly improving the extraction rate and purity of flavonoids and anthocyanins. Centrifugal separation in a centrifuge under low temperature conditions can effectively remove large particle impurities and prevent the target compounds from being degraded due to high temperature, thereby achieving effective removal of impurities and efficient evaporation of solvents. MIPs that specifically identify compounds are designed and synthesized based on the chemical structure characteristics of flavonoids and anthocyanins, ensuring highly selective adsorption capacity, which not only improves the purity of the product, but also provides high-quality basic materials for subsequent applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0068] Figure 1 The present invention is a flow chart of a method for extracting bayberry fruit core brass and anthocyanin;

[0069] Figure 2 The system diagram of a device for extracting bayberry kernel brass and anthocyanin. DETAILED DESCRIPTION

[0070] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and understandable, the specific implementation modes of the present invention are described in detail below in conjunction with the accompanying drawings.

[0071] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0072] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0073] Example 1

[0074] See also Figure 1-2 , which is the first embodiment of the present invention, provides an extraction device and method for bayberry fruit core brass and anthocyanin, comprising:

[0075] S1, prepare fresh bayberry fruit, wash it to remove surface dirt, and beat the whole fruit, and perform low-temperature freeze-drying on the fruit core to obtain a dried fruit core;

[0076] Furthermore, fresh bayberry fruits are selected, and a citric acid aqueous solution with a pH value of 3.5 and 5% salt is used to wash the bayberry fruits at room temperature to remove dirt, dust and insects on the surface;

[0077] The centrifuge is used to separate the bayberry and the pit. Under the centrifugal force generated by high-speed rotation, the heavier pit will settle at the bottom of the centrifuge tube, while the lighter pulp will remain on the upper layer.

[0078] The fruit core is placed in an ultra-low temperature refrigerator at -80℃ for rapid freezing, then transferred to a freeze dryer and gradually heated to room temperature under vacuum conditions. The entire drying process is maintained between -40℃ and -50℃, with the relative pressure set below 0.1mbar, until the moisture content is reduced to 5%, and the dried fruit core is obtained.

[0079] It should be noted that selecting fresh bayberry fruits and using a citric acid aqueous solution with a pH value of 3.5 combined with salt to wash them can not only effectively remove surface dirt, but also effectively remove insects and other diseases, ensuring sanitary conditions in the subsequent extraction process. The fruit cores are quickly frozen and freeze-dried under specific conditions, which not only retains the active ingredients to the maximum extent, but also makes the dried fruit cores easy to store and transport, while reducing the difficulty of the subsequent extraction process.

[0080] S2, placing the dried fruit core into a supercritical fluid extraction device, setting the pressure and temperature, and adding co-solvent ethanol to obtain an extract of flavonoids and anthocyanidin compounds;

[0081] Furthermore, the dried fruit core is placed into an extraction kettle of a supercritical fluid extraction device;

[0082] The supercritical fluid extraction device was set to an extraction pressure of 350 bar and an extraction temperature of 40 °C;

[0083] Add ethanol as a co-solvent to the device at a ratio not exceeding 5% by volume;

[0084] Turn on the supercritical fluid extraction device, start the extraction program, and set the extraction time to 60 minutes;

[0085] When the extraction is completed, a supercritical fluid extract of flavonoids and anthocyanidin compounds is obtained from the collection container;

[0086] The extraction kinetics-based optimization of supercritical fluid extraction process is introduced, and the expression is:

[0087]

[0088] Where E is the extraction efficiency, P is the extraction pressure, V co2 is the volume flow rate of supercritical carbon dioxide, R is the ideal gas constant, T is the absolute temperature, k eth anol is the ethanol enhancement factor, C target is the concentration of the target compound, and A is the mass transfer area.

[0089] It should be noted that setting specific extraction parameters (pressure of 350 bar and temperature of 40°C) and adding an appropriate amount of ethanol as a co-solvent are the results of optimizing the physicochemical properties of flavonoids and anthocyanins, aiming to improve the extraction efficiency while protecting heat-sensitive components. By introducing extraction kinetics, the operating conditions can be dynamically adjusted to achieve the best extraction effect, ensure the maximum release of target compounds, and reduce unnecessary impurities.

[0090] S3, using a centrifuge to remove large particle impurities from the extract of flavonoids and anthocyanin compounds, and using a rotary evaporator to evaporate the solvent to obtain a crude extract solution;

[0091] Furthermore, the supercritical fluid extract of flavonoid and anthocyanin compounds is transferred to a container dedicated to a centrifuge;

[0092] Select Eppendorf 5810R high-speed refrigerated centrifuge, set the speed to 3500 rpm, and maintain the temperature at 4°C;

[0093] The centrifugation time was set to 20 minutes to allow the suspended large particles of impurities to settle to the bottom of the container to form a precipitate, and a clarified extract was obtained;

[0094] Transfer the clarified extract to the evaporating flask of the rotary evaporator R-215, set the water bath temperature to 40°C, set the system vacuum to about 200 mbar, and set the rotation speed to 120 rpm;

[0095] Start the rotary evaporator and begin the evaporation process, and continue to monitor the evaporation progress until most of the solvent is removed and a small amount of concentrate remains;

[0096] When an appropriate amount of concentrated liquid is observed to remain in the evaporating flask, stop heating and release the vacuum state to restore the system to normal pressure;

[0097] Taking out the concentrated crude extract solution from the evaporating flask, transferring it to a clean storage container, and obtaining a crude extract solution;

[0098] During the centrifugal separation and rotary evaporation process, optimization based on physical and chemical principles was introduced, and the expression is:

[0099]

[0100] Among them, C final is the concentration of the final crude extract solution, C initial is the concentration of the initial extract, V initial is the initial volume, V final is the final volume, k is the evaporation rate constant, t is the evaporation time, F centrifuge is the centrifugal force, M impurities is the impurity mass, A filter is the effective filtration area.

[0101] It should be noted that centrifugal separation is performed in a low-temperature environment using an Eppendorf 5810R high-speed refrigerated centrifuge, which can not only effectively remove large particle impurities, but also prevent the target compound from being degraded due to high temperature. The mild conditions set by the rotary evaporator (40°C water bath temperature, 200mbar vacuum degree) help to remove most of the solvent without destroying the active ingredients to obtain a concentrate. By introducing physical and chemical principles, the concentration process can be precisely controlled to ensure the quality and purity of the final crude extract solution.

[0102] S4, selectively adsorbing the crude extract solution using a molecular imprinting polymer to obtain a purified target product;

[0103] Furthermore, molecular imprinting polymers that specifically recognize the compounds were designed and synthesized based on the chemical structural characteristics of flavonoids and anthocyanins;

[0104] MIPs were prepared by thermal polymerization under anhydrous conditions using template molecules quercetin as a representative of flavonoids, cyanidin as a representative of anthocyanins, functional monomer methacrylic acid, crosslinker ethylene glycol dimethacrylate and initiator;

[0105] The synthesized MIPs are filled into a solid phase extraction column;

[0106] The concentrated crude extract solution was slowly passed through the solid phase extraction column containing MIPs to allow the target flavonoids and anthocyanins in the solution to fully contact with the MIPs, and the flow rate was controlled at 0.5 mL / min;

[0107] Use low-polarity solvent water as eluent to remove non-target substances that are not adsorbed;

[0108] After confirming that the non-target substances have been completely eluted, switch to a high-polarity eluent, a methanol / water mixture, with a set ratio of 7:3;

[0109] Collect the desorbed liquid to obtain the purified target product.

[0110] It should be noted that the design and synthesis of MIPs that specifically recognize flavonoids and anthocyanins are completed based on the unique structural characteristics of these two compounds, ensuring highly selective adsorption capacity. By strictly controlling the flow rate of 0.5 mL / min and the selection of eluent (low polarity first and then high polarity), the target compounds can be recovered to the greatest extent while removing non-target substances. This process not only improves the purity of the product, but also provides high-quality basic materials for subsequent applications.

[0111] S5, using a natural antioxidant to stabilize the purified target product to obtain flavonoid and anthocyanin extracts;

[0112] Furthermore, a natural antioxidant vitamin C is selected and formulated into a 5%w aqueous solution according to the expected final product concentration;

[0113] Add the high-purity flavonoids and anthocyanins target products collected after desorption to the above antioxidant solution, adding 1-2 mL of antioxidant solution per 100 mL of target product;

[0114] Allow the mixed solution to stand at room temperature for 30 minutes to 1 hour to allow the antioxidant to form a complex with the target compound;

[0115] After stabilization, the mixed solution was filtered using a 0.45 μm filter membrane to remove precipitates or macromolecular impurities.

[0116] Use gentle stirring or ultrasonic-assisted methods to fully mix the two, and avoid loss or destruction of ingredients due to violent stirring;

[0117] The stabilized and filtered flavonoid and anthocyanin extracts were packaged into dark glass bottles and stored in a cool, dry, dark place at a temperature of 25°C and a humidity of 60%.

[0118] It should be noted that the use of natural antioxidant vitamin C and its preparation into an aqueous solution in a certain proportion not only enhances the stability of the product but also maintains its natural properties, meeting consumers' demand for healthy food. By allowing the mixed solution to stand for a period of time, the antioxidant and the target compound are fully combined to form a stable complex. In addition, filtering with a 0.45μm filter membrane can further remove possible precipitates or macromolecular impurities to ensure the clarity and quality of the final product. The product is packaged in dark glass bottles and stored in a suitable environment to extend the shelf life and ensure the stability and biological activity of the product throughout its life cycle.

[0119] This embodiment also provides an extraction device for bayberry fruit core brass and anthocyanin, comprising:

[0120] Pretreatment and low-temperature freeze-drying module, supercritical fluid extraction module, preliminary filtration and concentration module, molecular imprinting polymer adsorption module, stabilization treatment module and packaging and storage module;

[0121] The pretreatment and low-temperature freeze-drying module is located at the front end of the extraction equipment and is connected to the cleaning device through a conveyor belt, and is used to select and clean fresh bayberry fruits, peel them and remove the cores;

[0122] The pretreatment and low-temperature freeze-drying module comprises an ultra-low temperature refrigerator and a freeze dryer, which are connected by a pipeline and are used for rapid freezing and gradually heating under vacuum conditions to remove moisture, and finally obtain a dried fruit core;

[0123] The supercritical fluid extraction module is immediately after the pretreatment and low-temperature freeze-drying module and is physically connected via an automated feeding system to feed the dried fruit cores into the extraction kettle;

[0124] The supercritical fluid extraction module is equipped with a pressure regulating valve and a temperature controller, which can accurately set the extraction conditions of 350 bar pressure and 40 ° C temperature, and add an appropriate amount of ethanol as a co-solvent through the automatic liquid addition system to complete the efficient extraction of the target compound;

[0125] The preliminary filtration and concentration module is located after the SFE module, connected to the SFE module through a pumping system, and receives the extract flowing out of the SFE module;

[0126] The preliminary filtration and concentration module is equipped with a centrifuge and a rotary evaporator, the centrifuge is used to remove large particle impurities, and the rotary evaporator is responsible for evaporating the solvent under controlled conditions to obtain a crude extract solution;

[0127] The molecularly imprinted polymer adsorption module is located after the preliminary filtration and concentration module, and is connected to the former through a precision metering pump to receive the crude extract solution that has undergone preliminary treatment;

[0128] The molecularly imprinted polymer adsorption module has a built-in special solid phase extraction column filled with MIPs designed and synthesized for flavonoids and anthocyanins. The target compound is highly selectively adsorbed by slow flow, and then desorbed by using eluents of different polarities to collect the purified target product.

[0129] The stabilization treatment module is located after the MIPs adsorption module and is connected through a liquid transmission pipeline to receive the high-purity target product desorbed;

[0130] The stabilization treatment module is equipped with a mixing and stirring device and a constant temperature static zone, which can add natural antioxidants according to a predetermined ratio and ensure that the two are fully mixed and uniformly formed by gentle stirring or ultrasonic-assisted methods to form a stable complex;

[0131] The packaging and storage module is located after the stabilization treatment module and is connected via a sealed conveyor belt to receive the final product after stabilization treatment;

[0132] The stabilization processing module has an automatic packaging function, which can accurately distribute the product into dark glass bottles or other light-proof containers and store them in sealed containers.

[0133] This embodiment also provides a computer device, which is suitable for the method of extracting bayberry fruit core brass and anthocyanins, including: a memory and a processor; the memory is used to store computer executable instructions, and the processor is used to execute computer executable instructions to implement the method for extracting bayberry fruit core brass and anthocyanins as proposed in the above embodiment.

[0134] The computer device may be a terminal, and the computer device includes a processor, a memory, a communication interface, a display screen and an input device connected via a system bus. The processor of the computer device is used to provide computing and control capabilities. The memory of the computer device includes a non-volatile storage medium and an internal memory. The non-volatile storage medium stores an operating system and a computer program. The internal memory provides an environment for the operation of the operating system and the computer program in the non-volatile storage medium. The communication interface of the computer device is used to communicate with an external terminal in a wired or wireless manner, and the wireless manner can be achieved through Wi-Fi, an operator network, NFC (near field communication) or other technologies. The display screen of the computer device may be a liquid crystal display screen or an electronic ink display screen, and the input device of the computer device may be a touch layer covering the display screen, or a key, trackball or touchpad provided on the housing of the computer device, or an external keyboard, touchpad or mouse, etc.

[0135] This embodiment also provides a storage medium on which a computer program is stored. When the program is executed by a processor, the method for extracting brass and anthocyanins from bayberry core proposed in the above embodiment is implemented; the storage medium can be implemented by any type of volatile or non-volatile storage device or a combination thereof, such as static random access memory (Static Random Access Memory, referred to as SRAM), electrically erasable programmable read-only memory (Electrically Erasable Programmable Read-Only Memory, referred to as EEPROM), erasable programmable read-only memory (Erasable Programmable Read Only Memory, referred to as EPROM), programmable read-only memory (Programmable Red-Only Memory, referred to as PROM), read-only memory (Read-Only Memory, referred to as ROM), magnetic storage, flash memory, disk or optical disk.

[0136] In summary, by selecting fresh bayberry fruits and washing them with a citric acid aqueous solution with a pH value of 3.5 and salt, the surface dirt and pests such as insects can be removed, ensuring the quality and sanitary conditions of the raw materials. By loading the dried fruit cores into a supercritical fluid extraction device, setting precise pressure and temperature, and adding an appropriate amount of ethanol as a co-solvent through an automatic liquid addition system, efficient extraction of the target compounds can be achieved, and the extraction rate and purity of flavonoids and anthocyanins can be significantly improved. Centrifugal separation is performed in a centrifuge under low temperature conditions, which can effectively remove large particle impurities and prevent the target compounds from being degraded due to high temperature, achieving effective removal of impurities and efficient evaporation of solvents. MIPs that specifically recognize compounds are designed and synthesized according to the chemical structure characteristics of flavonoids and anthocyanins, ensuring highly selective adsorption capacity, which not only improves the purity of the product, but also provides high-quality basic materials for subsequent applications.

[0137] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A method for extracting bayberry fruit core brass and anthocyanins, characterized in that: include: Prepare fresh bayberry fruits, wash them to remove surface dirt, beat the whole fruits, and perform low-temperature freeze-drying on the fruit cores to obtain dried fruit cores; The dried fruit core is placed in a supercritical fluid extraction device, the pressure and temperature are set, and the co-solvent ethanol is added to obtain an extract of flavonoids and anthocyanidin compounds; The extract of flavonoids and anthocyanidins is centrifuged to remove large particle impurities, and the solvent is evaporated using a rotary evaporator to obtain a crude extract solution; The crude extract solution is selectively adsorbed by molecularly imprinted polymer to obtain the purified target product; The purified target product is stabilized by using an antioxidant from natural sources to obtain flavonoid and anthocyanidin extracts.

2. A method for extracting bayberry fruit core brass and anthocyanins according to claim 1, characterized in that: The method comprises preparing fresh bayberry fruits, washing them to remove surface dirt, peeling and removing the cores, and performing low-temperature freeze-drying treatment on the cores to obtain dried cores. The specific steps are as follows: Select fresh bayberry fruits, use a citric acid solution with a pH value of 3.5 and 5% salt, and wash the bayberry fruits at room temperature to remove dirt, dust and insects on the surface; The centrifuge is used to separate the bayberry and the pit. Under the centrifugal force generated by high-speed rotation, the heavier pit will settle at the bottom of the centrifuge tube, while the lighter pulp will remain on the upper layer. The fruit core is placed in an ultra-low temperature refrigerator at -80℃ for rapid freezing, then transferred to a freeze dryer and gradually heated to room temperature under vacuum conditions. The entire drying process is maintained between -40℃ and -50℃, with the relative pressure set below 0.1mbar, until the moisture content is reduced to 5%, and the dried fruit core is obtained.

3. A method for extracting bayberry fruit core brass and anthocyanins according to claim 2, characterized in that: The dried fruit core is placed in a supercritical fluid extraction device, the pressure and temperature are set, and the co-solvent ethanol is added to obtain an extract of flavonoids and anthocyanidin compounds. The specific steps are: The dried fruit core is placed into an extraction kettle of a supercritical fluid extraction device; The supercritical fluid extraction device was set to an extraction pressure of 350 bar and an extraction temperature of 40 °C; Add ethanol as a co-solvent to the device at a ratio not exceeding 5% by volume; Turn on the supercritical fluid extraction device, start the extraction program, and set the extraction time to 60 minutes; When the extraction is completed, a supercritical fluid extract of flavonoids and anthocyanidin compounds is obtained from the collection container; The extraction kinetics-based optimization of supercritical fluid extraction process is introduced, and the expression is: Where E is the extraction efficiency, P is the extraction pressure, V co2 is the volume flow rate of supercritical carbon dioxide, R is the ideal gas constant, T is the absolute temperature, k eth anol is the ethanol enhancement factor, C target is the concentration of the target compound, and A is the mass transfer area.

4. A method for extracting bayberry fruit core brass and anthocyanins according to claim 3, characterized in that: The method of using a centrifuge to remove large particle impurities from the extract of flavonoids and anthocyanin compounds and using a rotary evaporator to evaporate the solvent to obtain a crude extract solution comprises the following specific steps: transferring the supercritical fluid extract of flavonoid and anthocyanin compounds into a container dedicated to the centrifuge; Select Eppendorf 5810R high-speed refrigerated centrifuge, set the speed to 3500 rpm, and maintain the temperature at 4°C; The centrifugation time was set to 20 minutes to allow the suspended large particles of impurities to settle to the bottom of the container to form a precipitate, and a clarified extract was obtained; Transfer the clarified extract to the evaporating flask of the rotary evaporator R-215, set the water bath temperature to 40°C, set the system vacuum to about 200 mbar, and set the rotation speed to 120 rpm; Start the rotary evaporator and begin the evaporation process, and continue to monitor the evaporation progress until most of the solvent is removed and a small amount of concentrate remains; When an appropriate amount of concentrated liquid is observed to remain in the evaporating flask, stop heating and release the vacuum state to restore the system to normal pressure; The concentrated crude extract solution is taken out from the evaporating flask, transferred to a clean storage container, and a crude extract solution is obtained.

5. A method for extracting bayberry fruit core brass and anthocyanins according to claim 4, characterized in that: During the centrifugal separation and rotary evaporation, optimization based on physical and chemical principles is introduced, and the expression is: Among them, C final is the concentration of the final crude extract solution, C initial is the concentration of the initial extract, V initial is the initial volume, V final is the final volume, k is the evaporation rate constant, t is the evaporation time, F centrifuge is the centrifugal force, M impurities is the impurity mass, A filter is the effective filtration area.

6. A method for extracting bayberry fruit core brass and anthocyanins according to claim 5, characterized in that: The molecularly imprinted polymer is used to selectively adsorb the crude extract solution to obtain the purified target product, and the specific steps are: According to the chemical structure characteristics of flavonoids and anthocyanins, molecular imprinting polymers that specifically recognize compounds are designed and synthesized; MIPs were prepared by thermal polymerization under anhydrous conditions using template molecules quercetin as a representative of flavonoids, cyanidin as a representative of anthocyanins, functional monomer methacrylic acid, crosslinker ethylene glycol dimethacrylate and initiator; The synthesized MIPs are filled into a solid phase extraction column; The concentrated crude extract solution was slowly passed through the solid phase extraction column containing MIPs to allow the target flavonoids and anthocyanins in the solution to fully contact with the MIPs, and the flow rate was controlled at 0.5 mL / min; Use low-polarity solvent water as eluent to remove non-target substances that are not adsorbed; After confirming that the non-target substances have been completely eluted, switch to a high-polarity eluent, a methanol / water mixture, with a set ratio of 7:3; Collect the desorbed liquid to obtain the purified target product.

7. A method for extracting bayberry fruit core brass and anthocyanins according to claim 6, characterized in that: The method of using a natural antioxidant to stabilize the purified target product to obtain flavonoid and anthocyanidin extracts comprises the following specific steps: Selecting a natural antioxidant vitamin C, and preparing it into a 5% w aqueous solution according to the expected final product concentration; Add the high-purity flavonoids and anthocyanins target products collected after desorption to the above antioxidant solution, adding 1-2 mL of antioxidant solution per 100 mL of target product; Allow the mixed solution to stand at room temperature for 30 minutes to 1 hour to allow the antioxidant to form a complex with the target compound; After stabilization, the mixed solution was filtered using a 0.45 μm filter membrane to remove precipitates or macromolecular impurities. Use gentle stirring or ultrasonic-assisted methods to fully mix the two, and avoid loss or destruction of ingredients due to violent stirring; The stabilized and filtered flavonoid and anthocyanin extracts were packaged into dark glass bottles and stored in a cool, dry, dark place at a temperature of 25°C and a humidity of 60%.

8. An extraction device for bayberry fruit core brass and anthocyanins, according to the method for extracting bayberry fruit core brass and anthocyanins according to any one of claims 1 to 7, characterized in that: include: Pretreatment and low-temperature freeze-drying module, supercritical fluid extraction module, preliminary filtration and concentration module, molecular imprinting polymer adsorption module, stabilization treatment module and packaging and storage module; The pretreatment and low-temperature freeze-drying module is located at the front end of the extraction equipment and is connected to the cleaning device through a conveyor belt, and is used to select and clean fresh bayberry fruits, peel them and remove the cores; The pretreatment and low-temperature freeze-drying module comprises an ultra-low temperature refrigerator and a freeze dryer, which are connected by a pipeline and are used for rapid freezing and gradually heating under vacuum conditions to remove moisture, and finally obtain a dried fruit core; The supercritical fluid extraction module is immediately after the pretreatment and low-temperature freeze-drying module and is physically connected via an automated feeding system to feed the dried fruit cores into the extraction kettle; The supercritical fluid extraction module is equipped with a pressure regulating valve and a temperature controller, which can accurately set the extraction conditions of 350 bar pressure and 40 ° C temperature, and add an appropriate amount of ethanol as a co-solvent through the automatic liquid addition system to complete the efficient extraction of the target compound; The preliminary filtration and concentration module is located after the SFE module, connected to the SFE module through a pumping system, and receives the extract flowing out of the SFE module; The preliminary filtration and concentration module is equipped with a centrifuge and a rotary evaporator, the centrifuge is used to remove large particle impurities, and the rotary evaporator is responsible for evaporating the solvent under controlled conditions to obtain a crude extract solution; The molecularly imprinted polymer adsorption module is located after the preliminary filtration and concentration module, and is connected to the former through a precision metering pump to receive the crude extract solution that has undergone preliminary treatment; The molecularly imprinted polymer adsorption module has a built-in special solid phase extraction column filled with MIPs designed and synthesized for flavonoids and anthocyanins. The target compound is highly selectively adsorbed by slow flow, and then desorbed by using eluents of different polarities to collect the purified target product. The stabilization treatment module is located after the MIPs adsorption module and is connected through a liquid transmission pipeline to receive the high-purity target product desorbed; The stabilization treatment module is equipped with a mixing and stirring device and a constant temperature static zone, which can add natural antioxidants according to a predetermined ratio and ensure that the two are fully mixed and uniformly formed by gentle stirring or ultrasonic-assisted methods to form a stable complex; The packaging and storage module is located after the stabilization treatment module and is connected via a sealed conveyor belt to receive the final product after stabilization treatment; The stabilization processing module has an automatic packaging function, which can accurately distribute the product into dark glass bottles or other light-proof containers and store them in sealed containers.

9. A computer device comprising a memory and a processor, wherein the memory stores a computer program, wherein: When the processor executes the computer program, the steps of the method for extracting bayberry fruit core brass and anthocyanins according to any one of claims 1 to 7 are implemented.

10. A computer-readable storage medium having a computer program stored thereon, characterized in that: When the computer program is executed by a processor, the steps of the method for extracting bayberry fruit core brass and anthocyanins as described in any one of claims 1 to 7 are implemented.

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