Soil conditioner with combination of saline-alkaline tolerant fungicide and xanthoceras sorbifolia bunge shells and preparation method of soil conditioner

Abstract

The invention designs a soil conditioner with combination of a saline-alkaline tolerant microbial agent and shiny-leaved yellowhorn shell and a preparation method of the soil conditioner. The soil conditioner comprises shiny-leaved yellowhorn shell powder, saline-alkaline tolerant microbial agent fermentation liquor, peat and humic acid. The mass ratio of the xanthoceras sorbifolia bunge shell powder to the saline-alkaline tolerant microbial agent fermentation liquor is (1-20): 1; the mass ratio of the shiny-leaved yellowhorn shell powder to the peat is 1: (0.1-0.5); the mass ratio of the xanthoceras sorbifolia bunge shell powder to the humic acid is 1: (0.05-0.2); the saline-alkaline tolerant microbial agent fermentation liquor is prepared from one or more of bacillus subtilis, bacillus megatherium, halophilus coryneform, mycobacillus halophilus, halophilic bacillus, saline-alkaline philic bacillus, alcalimonas amyloliquefaciens and saline-alkaline amyloliquefaciens; and the saline-alkaline tolerant microbial agent fermentation liquor is prepared from one or more of bacillus subtilis, bacillus megatherium, halophilus coryneform, mycobacillus halophilus, halophilic bacillus and saline-alkaline amyloliquefaciens. The soil conditioner designed by the invention is applied to a plough layer of the saline-alkali soil in manners of broadcasting, ploughing and the like, and can increase the quantity of saline-alkali tolerant beneficial microorganisms in the saline-alkali soil and the content of long-acting organic matters, so that the problem that microorganisms and organic matters in the saline-alkali soil are rare can be solved.

Description

Technical Field

[0001] The invention belongs to the field of soil improvement, and particularly relates to a soil improver of a salt-alkali tolerant bacterial agent combined with Xanthoceras sorbifolia husk and a preparation method thereof. Background Art

[0002] Salinized soil is widely distributed throughout the world, and the problem of soil salinization is a global problem. The area of ​​saline-alkali land in the world is about 950 million hectares, accounting for about 10% of the total land area. Saline-alkali land resources are important land resources in my country. Therefore, improving the production potential of developed saline-alkali land and developing and utilizing undeveloped saline-alkali land are of great significance to expanding China's arable land area and ensuring food security.

[0003] Soil salinization will lead to a decrease in soil fertility, difficulty in plant roots absorbing water, difficulty in crop growth or a significant reduction in yield or even abandonment of cultivation, which seriously affects the sustainable development of my country's agriculture and forestry. Therefore, in the current situation of shrinking cultivated land and severe shortage of land resources in my country, improving saline-alkali soil and comprehensively utilizing saline-alkali land has become an important national strategic task and urgent need.

[0004] Generally speaking, the main factors in improving saline-alkali soil include soil pH, salt content and groundwater level, etc. However, there are several key issues that deserve attention in improving saline-alkali soil and planting crops on saline-alkali soil: ① soil organic matter deficiency, especially water-soluble organic matter; ② small number of soil microorganisms and imbalanced community structure, which are prone to soil-borne diseases.

[0005] In the past, microbial agents were often added to saline-alkali soils in the form of bacterial powder or liquid. On the one hand, most of these bacterial agents and powders were microbial strains that were not tolerant to salt and alkali. On the other hand, these microorganisms lacked effective carriers and were difficult to survive in the soil and function for a long time. Summary of the invention

[0006] In order to solve the above problems, the present application designs a soil conditioner of salt-alkali tolerant bacteria agent combined with Xanthoceras sorbifolia shell and its preparation method, in order to provide salt-alkali tolerant microbial strains and their effective carriers, thereby solving the problems of scarce saline-alkali soil microorganisms, difficult survival of exogenous strains and lack of organic matter in saline-alkali soil.

[0007] Xanthoceras sorbifolia Bunge is a woody oil plant unique to my country. Due to its wide ecological adaptability, it is distributed or planted in 14 provinces (municipalities, autonomous regions) in northern my country. In the 21st century, after Xanthoceras sorbifolia Bunge was listed as a key bioenergy tree species for development in my country, the planned planting area in various provinces and regions has increased rapidly, and incomplete statistics have reached more than one million hectares.

[0008] Generally speaking, the fruit harvested from Xanthoceras sorbifolia is composed of pericarp and seeds, and the seeds include seed coat and kernel. In terms of unit weight, the pericarp (also known as Xanthoceras sorbifolia shell) accounts for about 48% of the fruit, the seed coat about 25%, and the kernel about 27%. The oil content in the kernel is 55% to 65%, which shows that oil accounts for about 15% of the fruit harvest. According to this ratio, for every portion of Xanthoceras sorbifolia oil produced, more than 5 times the shell and seed coat and other residues will be produced. Previously, no effective way to utilize the shell and seed coat and other residues has been found. The shell and seed coat of Xanthoceras sorbifolia are porous structures with strong adsorption capacity. At the same time, the shell of Xanthoceras sorbifolia is also a potential resource of excellent organic matter. If the resource utilization problem of the residues from Xanthoceras sorbifolia processing cannot be solved, it will not only cause a huge waste of raw material resources, but also bring huge pressure to the environment.

[0009] Salt-alkali tolerant microorganisms adapt to high saline-alkali environments by changing membrane transport fluxes and maintaining normal cell life activities. They can help plants rebuild ion and osmotic balance, reduce cell damage caused by stress responses, and restore plant growth under salt stress conditions. In addition, salt-alkali tolerant bacteria have different effects in phosphorus solubilization, potassium solubilization, and nitrogen fixation, providing species resources and theoretical basis for microbial improvement of saline-alkali land.

[0010] Bacillus is the most studied and commercialized agricultural salt-tolerant microorganism. It can tolerate salt content ≤15wt% (>15% is classified as an extremely salt-tolerant microorganism), and can adapt to soil pH range between 5.0-9.5. The dormant spores it produces have extremely strong stress tolerance, usually have growth-promoting effects such as dissolving phosphorus and potassium, resisting diseases, and secreting plant growth regulators. The yield increase effect of application can reach about 5.0% for rice, more than 6.6% for corn, and more than 7.9% for soybeans. They are the engines of biogenic elements and micronutrient migration and transformation in the soil-plant system. The decomposition and accumulation of organic matter in the soil, nitrogen transformation, release of nutrients such as phosphorus, potassium, iron, and zinc, supply of plant nutrition to stimulate plant growth, and inhibition of pathogens and improvement of soil are all closely related to the activities of Bacillus, including Bacillus megaterium, Bacillus amyloliquefaciens, Bacillus firmus, Bacillus licheniformis, Bacillus brevis, etc. In saline-alkali land, Bacillus can intensify the biochemical processes of saline-alkali soil and improve the activity of soil enzymes by secreting organic acids, enzymes and other substances, thereby increasing the effective nutrients in the soil, lowering the soil pH, increasing soil water-stable aggregates, and reducing the salt content of the cultivated layer. Therefore, Bacillus can play an extremely important role in improving the rhizosphere microhabitat of crops in saline-alkali land.

[0011] It can be seen that salt-alkali tolerant microorganisms, especially Bacillus, have many advantages in improving saline-alkali soil, but in the implementation process, due to the lack of adsorption carriers and slow-release measures, it is difficult for them to fully play their role in the soil. Therefore, the present application designs a soil conditioner of salt-alkali tolerant bacteria combined with Xanthoceras sorbifolia shell, the soil conditioner includes: Xanthoceras sorbifolia shell powder, salt-alkali tolerant bacteria fermentation liquid, peat, humic acid;

[0012] The mass ratio of the Xanthoceras sorbifolia shell powder to the salt-alkali-tolerant bacteria agent fermentation liquid is 1 to 20:1;

[0013] The mass ratio of the Xanthoceras sorbifolia shell powder to peat is 1:0.1-0.5;

[0014] The mass ratio of the Xanthoceras sorbifolia shell powder to humic acid is 1:0.05-0.2.

[0015] Preferably, the active strains in the salt-alkali tolerant bacterial agent fermentation broth include one or more strains of Bacillus subtilis (CGMCC1.3358), Bacillus megaterium (CGMCC1.7339), Halobellus clavatus (CGMCC1.10118), Virgibacillus salinus (CGMCC1.10449), Bacillus saliphilus (CGMCC1.15441), Alkalibacillus haloalkaliphilus (CGMCC1.15274), Alkalimonasamylolytica (CGMCC1.3430), and Natronococcus amylolyticus (CGMCC1.6249).

[0016] This number is the strain preservation number of the China General Microbiological Culture Collection Center (CGMCC).

[0017] Preferably, the pH value of the salt-alkali tolerant bacteria fermentation broth ranges from 0.3 to 9.0, and the total effective viable count is greater than 200 million CFU / ml.

[0018] Preferably, the size of the Xanthoceras sorbifolia husk powder is 5-200 mesh.

[0019] A method for preparing a soil conditioner of a salt-alkali tolerant bacterial agent and Xanthoceras sorbifolia husks comprises the following steps:

[0020] Step S1, preparing salt-alkali tolerant bacterial agent fermentation liquid;

[0021] Step S2, crushing the Xanthoceras sorbifolia shell into 5-200 meshes;

[0022] Step S3, mixing the Xanthoceras sorbifolia shell powder and the salt-alkali-tolerant bacteria agent fermentation liquid in a ratio of 1 to 20:1 to form a preliminary mixture;

[0023] Step S4, mixing the preliminary mixture, peat and humic acid in a ratio of 1:0.1-0.5:0.05-0.2 to form a mid-step mixture;

[0024] Step S5, adding water and a binder to the intermediate step mixture, and making a granular soil conditioner by extrusion granulation; or air-drying or drying the intermediate step mixture to make a powdered soil conditioner.

[0025] Preferably, in step S1, the method for preparing the salt-alkali tolerant bacterial agent fermentation broth comprises:

[0026] Step S11, placing a carbon source, a nitrogen source, and trace elements in a fermentation system of the strain;

[0027] Step S12, after high temperature sterilization and cooling to 28-32° C., inoculating one or more of Bacillus subtilis, Bacillus megaterium, Bacillus halogenus, Bacillus halogenus, Bacillus halophilus, Bacillus halophilus, Alkalimonas amyloliquefaciens, and Alkalimonas amyloliquefaciens;

[0028] Step S13: After fermentation for 24 to 96 hours, a salt-alkali tolerant bacterial agent fermentation liquid is formed.

[0029] Preferably, in step S5, the binder includes diatomaceous earth.

[0030] Preferably, in step S13, the ventilation volume of the fermentation is a ratio of the volume of compressed air introduced per minute to the volume of the fermentation liquid of 1:0.2-0.6, and the fermentation temperature is 28-32°C.

[0031] Preferably, in step S11, the carbon source includes: glucose, fructose, maltose, sucrose, molasses, organic acid; the nitrogen source includes: urea, ammonia, nitrate, peptone, yeast extract; and the trace elements include: sodium, potassium, magnesium, iron.

[0032] The advantages and effects of this application are as follows:

[0033] 1. The soil conditioner of salt-alkali-tolerant bacteria agent combined with Xanthoceras sorbifolia shell designed by the present application is formed by mixing Xanthoceras sorbifolia shell powder, salt-alkali-tolerant bacteria agent fermentation liquid, peat, and humic acid, and applied to the saline-alkali land arable layer by broadcasting combined with tillage, etc. The application amount per mu each time is 10 to 500 kg, and the total number of applications of the soil conditioner during the crop growing season is 1 to 3 times. Through the above design, the present application can reduce the pH value of saline-alkali soil and increase the organic matter content of soil.

[0034] 2. The salt- and alkali-tolerant bacterial agent combined with Xanthoceras sorbifolia shell soil conditioner designed in the present application uses Xanthoceras sorbifolia shell, which is the processing residue of Xanthoceras sorbifolia oil, and utilizes the Xanthoceras sorbifolia shell as a resource. On the one hand, it is used as an important source of supplementing soil organic matter, which can significantly increase soil organic matter. On the other hand, Xanthoceras sorbifolia shell, as an adsorptive carrier with strong adsorption capacity, can adsorb the salt- and alkali-tolerant bacterial agent.

[0035] 3. The salt-alkali tolerant bacterial agent designed in the present application and the soil improver of Xanthoceras sorbifolia husk use the salt-alkali tolerant bacterial agent, which can increase the number of salt-alkali tolerant microorganisms in the soil and improve the structure of soil microbial flora on the one hand; on the other hand, it can provide a large amount of organic matter for saline-alkali soil, thereby achieving efficient improvement of alkaline soil.

[0036] 4. The salt-alkali-tolerant bacteria agent combined with Xanthoceras sorbifolia shell soil improver designed in the present application is a mixture of salt-alkali-tolerant bacteria agent, humic acid, peat, and Xanthoceras sorbifolia shell, forming a diversified combination of small molecules, medium molecules, large molecules to super-large molecules, forming a reasonable distribution of organic matter, and increasing the content of various organic matter components such as fast-acting, medium-acting, and long-acting in saline-alkali soils.

[0037] The above description is only an overview of the technical solution of the present application. In order to more clearly understand the technical means of the present application so that it can be implemented in accordance with the contents of the specification, and to make the above and other purposes, features and advantages of the present application more obvious and easy to understand, the following is a detailed description of the preferred embodiments of the present application in conjunction with the accompanying drawings as follows.

[0038] Based on the detailed description of the specific embodiments of the present application in combination with the accompanying drawings below, those skilled in the art will become more aware of the above and other objects, advantages and features of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings required for use in the embodiments or the prior art descriptions are briefly introduced below. Obviously, the drawings described below are some embodiments of the present application. For ordinary technicians in this field, other drawings can also be obtained based on these drawings without creative work. In all drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, each element or part is not necessarily drawn according to the actual scale.

[0040] Figure 1 A flow chart for preparing a soil conditioner of a salt-alkali tolerant bacterial agent combined with Xanthoceras sorbifolia shell designed for this application;

[0041] Figure 2 The flow chart of the preparation of the salt-alkali tolerant bacterial fermentation broth designed for this application; DETAILED DESCRIPTION

[0042] To make the purpose, technical scheme and advantages of the embodiment of the present application clearer, the technical scheme in the embodiment of the present application will be clearly and completely described below in conjunction with the accompanying drawings in the embodiment of the present application. Obviously, the described embodiment is a part of the embodiment of the present application, rather than all of the embodiments. In the following description, specific details such as specific configuration and components are provided only to help fully understand the embodiments of the present application. Therefore, it should be clear to those skilled in the art that various changes and modifications can be made to the embodiments described herein without departing from the scope and spirit of the present application. In addition, for clarity and brevity, the description of known functions and structures is omitted in the embodiment.

[0043] It should be understood that the references to "one embodiment" or "this embodiment" throughout the specification mean that the specific features, structures, or characteristics associated with the embodiment are included in at least one embodiment of the present application. Therefore, the references to "one embodiment" or "this embodiment" appearing throughout the specification do not necessarily refer to the same embodiment. In addition, these specific features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.

[0044] In addition, the present application may repeat reference numerals and / or letters in different examples. This repetition is for the purpose of simplicity and clarity, and does not in itself indicate the relationship between the various embodiments and / or settings discussed.

[0045] The term "and / or" in this article is only a description of the association relationship of associated objects, indicating that there can be three relationships. For example, A and / or B can mean: A exists alone, B exists alone, and A and B exist at the same time. The term " / and" in this article describes another type of association object relationship, indicating that there can be two relationships. For example, A / and B can mean: A exists alone, and A and B exist alone. In addition, the character " / " in this article generally indicates that the previous and next associated objects are in an "or" relationship.

[0046] The term "at least one" in this article is merely a description of the association relationship of associated objects, indicating that there may be three relationships. For example, at least one of A and B can mean: A exists alone, A and B exist at the same time, and B exists alone.

[0047] It should also be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprises" or any other variations thereof are intended to cover non-exclusive inclusions.

[0048] Example 1

[0049] This embodiment mainly introduces the composition of a soil conditioner composed of a salt-alkali tolerant bacterial agent and Xanthoceras sorbifolia shells, including: Xanthoceras sorbifolia shell powder, salt-alkali tolerant bacterial agent fermentation liquid, peat, and humic acid;

[0050] The mass ratio of the Xanthoceras sorbifolia shell powder to the salt-alkali-tolerant bacteria agent fermentation liquid is 1 to 20:1;

[0051] The mass ratio of the Xanthoceras sorbifolia shell powder to peat is 1:0.1-0.5;

[0052] The mass ratio of the Xanthoceras sorbifolia shell powder to humic acid is 1:0.05-0.2.

[0053] These salt-alkali tolerant bacteria can release hydrogen ions through certain chemical reactions in the soil, reduce the soil pH, increase the soil organic matter and nutrient content, promote the growth and reproduction of soil microorganisms, and alleviate soil salinization, hardening and other problems. Salt-alkali tolerant bacteria can improve soil structure and increase soil porosity, thereby improving soil permeability and aeration, thereby promoting the release of inorganic phosphorus, iron, zinc and other nutrients locked in the soil, and increasing the chances of plants absorbing these elements. In addition, salt-alkali tolerant bacteria can also improve soil texture, structure and aeration through processes such as dissolving minerals and decomposing organic matter, improve soil fertility and water use efficiency, and thus promote crop growth. Salt-alkali tolerant bacteria can be used as a plant growth regulator to promote plant germination, growth and disease resistance by providing the nutrients and water required by plants. At the same time, they can also promote photosynthesis and respiration, thereby improving the utilization rate of light energy and growth rate of plants. In addition, salt-alkali tolerant bacteria can increase the sugar content, vitamin content and color brightness of fruits, increase crop yield and quality, and make crops more nutritious and marketable.

[0054] Peat is a natural swamp product formed over thousands of years. It contains high organic matter, humic acid and nutrients, has good aeration properties, is light in weight, retains water and fertilizer, and is conducive to microbial activity.

[0055] Humic acid is a macromolecular organic substance, which is formed and accumulated by the decomposition and transformation of animal and plant remains, mainly plant remains, by microorganisms and a series of geochemical processes. 2 ~10 6 Dalton. Due to its widespread existence, it has a great impact on the earth, involving carbon cycle, mineral migration and accumulation, soil fertility, ecological balance and other aspects. The total amount of humic acid contained in the soil is the largest, but its content in the soil is less than 1% on average. The basic structure of humic acid macromolecules is aromatic rings and alicyclic rings, with functional groups such as carboxyl, hydroxyl, carbonyl, quinone, and methoxy groups attached to the rings.

[0056] The main components of the Xanthoceras sorbifolia shell are ultra-large molecular cellulose and lignin, which are difficult to decompose in the short term. The advantage of this arrangement is that it provides long-lasting organic matter to loosen the saline-alkali soil and increase air permeability and water retention. Peat and humic acid are auxiliary ingredients that also play a role in improving the soil and promoting plant growth.

[0057] Furthermore, the salt-alkali tolerant bacterial fermentation broth comprises one or more of Bacillus subtilis, Bacillus megaterium, Bacillus rod-shaped, Bacillus halogenus, Bacillus halophilus, Bacillus halophilus, Bacillus amyloliquefaciens, and Bacillus halophilus.

[0058] Furthermore, the pH value of the salt-alkali tolerant bacterial agent fermentation liquid ranges from 0.3 to 9.0, and the effective viable bacteria count is greater than 200 million CFU / ml. The advantage of this arrangement is that it improves the acid-base neutralization effect (reducing the pH value of saline-alkali soil by 0.1-0.3), greatly increases the number and activity of salt-alkali tolerant bacterial agents of growth-promoting bacteria in alkaline soil, thereby making the barren alkaline soil relatively fertile and rapidly improving the survival rate and biomass of plants.

[0059] Furthermore, the size of the Xanthoceras sorbifolia shell powder is 5 to 200 meshes.

[0060] The present application uses the Xanthoceras sorbifolia shell, which is the processing residue of Xanthoceras sorbifolia oil, and utilizes the Xanthoceras sorbifolia shell as a resource. On the one hand, it is used as an important source of supplementing soil organic matter, which can significantly increase soil organic matter. On the other hand, the Xanthoceras sorbifolia shell, as an adsorptive carrier with strong adsorption capacity, can adsorb salt-alkali resistant bacterial agents.

[0061] Example 2

[0062] Based on Example 1, this example mainly introduces a method for preparing a soil conditioner of a salt-alkali tolerant bacterial agent combined with Xanthoceras sorbifolia shell, comprising the following steps:

[0063] Step S1, preparing salt-alkali tolerant bacterial agent fermentation liquid;

[0064] Step S2, crushing the Xanthoceras sorbifolia shell into 5-200 meshes;

[0065] Step S3, mixing the Xanthoceras sorbifolia shell powder and the salt-alkali-tolerant bacteria agent fermentation liquid in a ratio of 1 to 20:1 to form a preliminary mixture;

[0066] Step S4, mixing the preliminary mixture, peat and humic acid in a ratio of 1:0.1-0.5:0.05-0.2 to form a mid-step mixture;

[0067] Step S5, adding water and a binder to the intermediate step mixture, and making a granular soil conditioner by extrusion granulation; or air-drying or drying the intermediate step mixture to make a powdered soil conditioner.

[0068] Furthermore, in step S1, the method for preparing the salt-alkali tolerant bacterial agent fermentation broth comprises:

[0069] Step S11, placing a carbon source, a nitrogen source, and trace elements in a fermentation system of the strain;

[0070] Step S12, after high temperature sterilization and cooling to 28-32° C., inoculating one or more of Bacillus subtilis, Bacillus megaterium, Bacillus halogenus, Bacillus halogenus, Bacillus halophilus, Bacillus halophilus, Alkalimonas amyloliquefaciens, and Alkalimonas amyloliquefaciens;

[0071] Step S13: After fermentation for 24 to 96 hours, a salt-alkali tolerant bacterial agent fermentation liquid is formed.

[0072] Furthermore, in step S5, the binder includes diatomaceous earth.

[0073] Furthermore, in step S13, the ventilation volume of the fermentation is 1:0.2-0.6 of the volume of compressed air introduced per minute to the volume of the fermentation liquid, and the fermentation temperature is 28-32°C.

[0074] Furthermore, in step S11, the carbon source includes: glucose, fructose, maltose, sucrose, molasses, and organic acid; the nitrogen source includes: urea, ammonia, nitrate, peptone, and yeast extract; and the trace elements include: sodium, potassium, magnesium, and iron.

[0075] Furthermore, the application amount of the soil conditioner is 10 to 500 kg per mu, and the application frequency of the soil conditioner during the crop growing season is 1 to 3 times. Through the above design, the present application can increase the soil, reduce the pH value of saline-alkali soil, and increase the organic matter content of the soil.

[0076] The soil conditioner designed in the present application mixes the fermentation liquid of salt-alkali tolerant bacteria, humic acid, peat and Xanthoceras sorbifolia husk to form a diversified combination of small molecules, medium molecules, large molecules to super large molecules, forms a reasonable distribution of organic matter, and increases the content of various organic matter components such as fast-acting, medium-acting and long-acting in saline-alkali soil.

[0077] The present application designs a soil conditioner of salt-alkali tolerant bacteria agent combined with Xanthoceras sorbifolia shell, including: Xanthoceras sorbifolia shell powder, salt-alkali tolerant bacteria agent fermentation liquid, peat and humic acid; the mass ratio of Xanthoceras sorbifolia shell powder to salt-alkali tolerant bacteria agent fermentation liquid is 1-20:1; the mass ratio of the Xanthoceras sorbifolia shell powder to peat is 1:0.1-0.5; the mass ratio of the Xanthoceras sorbifolia shell powder to humic acid is 1:0.05-0.2; the salt-alkali tolerant bacteria agent fermentation liquid includes: one or more of Bacillus subtilis, Bacillus megaterium, Beautiful Halobacterium rod-shaped, Bacillus halogenus, Bacillus halophilus, Bacillus halophilus, Alkalimonas amyloliquefaciens, and Alkali halophilus amyloliquefaciens. The soil conditioner designed in the present application is applied to the cultivated layer of saline-alkali land by broadcasting combined with plowing, etc., which can increase the number of salt-alkali tolerant beneficial microorganisms and the content of long-term organic matter in saline-alkali soil, so the present application can solve the problem of scarce microorganisms and organic matter in saline-alkali soil.

[0078] Example 3

[0079] Based on Example 1 and Example 2, this example mainly introduces the first optimized design of a soil conditioner of a salt-alkali tolerant bacterial agent combined with Xanthoceras sorbifolia husk.

[0080] After the salt-alkali tolerant bacteria fermentation liquid and Xanthoceras sorbifolia shell powder are mixed in a ratio of 1:6, the "salt-alkali tolerant bacteria fermentation liquid + Xanthoceras sorbifolia shell powder mixture", peat and humic acid are mixed in a ratio of 1:0.2:0.1. According to the water content of the above materials, appropriate amount of water and binder (such as diatomaceous earth) are added, and a cylindrical or spherical granular saline-alkali soil conditioner is prepared by extrusion granulation.

[0081] In the test experiment, the pH value of saline-alkaline soil was 7.8. The application rate of soil conditioner was 150 kg per mu, which reduced the soil pH value by 0.1 (the pH value of the plant rhizosphere microenvironment decreased by 0.2-0.3), increased the soil organic matter from 7.1 g / kg to 7.5 g / kg, and significantly increased the number of soil bacteria, especially the number of Bacillus, from 2.3×10 4 CFU / g increased to 5.4×10 4 CFU / g.

[0082] Example 4

[0083] Based on Example 1 and Example 2, this example mainly introduces the second optimized design of the soil conditioner of salt-alkali tolerant bacteria agent combined with Xanthoceras sorbifolia husk.

[0084] After the salt-alkali tolerant bacteria fermentation liquid and Xanthoceras sorbifolia shell powder are mixed in a ratio of 1:10, the "salt-alkali tolerant bacteria fermentation liquid + Xanthoceras sorbifolia shell powder mixture", peat and humic acid are mixed in a ratio of 1:0.3:0.4. According to the water content of the above materials, appropriate amount of water and binder (such as diatomaceous earth) are added, and a cylindrical or spherical granular saline-alkali soil conditioner is prepared by extrusion granulation.

[0085] In Example 4, the pH value of the saline-alkaline soil was 8.3, and the application rate of the soil conditioner was 300 kg per mu, which reduced the soil pH by 0.24 (the pH value of the plant rhizosphere microenvironment decreased by 0.4-0.5), increased the soil organic matter from 5.3 g / kg to 6.7 g / kg, and increased the number of soil Bacillus by one order of magnitude from 1.7×10 3 CFU / g increased by 2.1×10 4 CFU / g.

[0086] The above description is only the preferred embodiment of the present invention, which does not limit the protection scope of the present invention. For those skilled in the art, the present invention can be modified and varied in various ways. Within the spirit and principle of the present invention, any change, modification, replacement, integration and parameter change of these embodiments by conventional substitution or capable of achieving the same function without departing from the principle and spirit of the present invention shall fall within the protection scope of the present invention.

Claims

1. A soil conditioner comprising a salt-alkali tolerant bacterial agent and Xanthoceras sorbifolia husk, characterized in that: Soil conditioners include: Xanthoceras sorbifolia shell powder, fermentation liquid of salt-alkali tolerant bacteria, peat, humic acid; The mass ratio of the Xanthoceras sorbifolia shell powder to the salt-alkali tolerant bacteria agent fermentation liquid is 1 to 20:1; The mass ratio of the Xanthoceras sorbifolia shell powder to peat is 1:0.1-0.5; The mass ratio of the Xanthoceras sorbifolia shell powder to humic acid is 1:0.05-0.

2.

2. The soil conditioner of a salt-alkali tolerant bacterial agent combined with Xanthoceras sorbifolia husk according to claim 1, characterized in that: The active strains in the fermentation liquid of the salt-alkali tolerant bacteria agent include one or more strains of Bacillus subtilis, Bacillus megaterium, Bacillus halogenus, Bacillus halogenus, Bacillus halophilus, Bacillus halophilus, Bacillus amyloliquefaciens, and Bacillus halophilus.

3. A soil conditioner comprising a salt-alkali tolerant bacterial agent and Xanthoceras sorbifolia husk according to any one of claims 1 or 2, characterized in that: The pH value of the salt-alkali resistant bacterial agent fermentation liquid ranges from 0.3 to 9.0, and the total effective viable bacteria count is greater than 200 million CFU / ml.

4. A soil conditioner comprising a salt-alkali tolerant bacterial agent and Xanthoceras sorbifolia husk according to any one of claims 1, 2 or 3, characterized in that: The size of the Xanthoceras sorbifolia shell powder is 5 to 200 meshes.

5. The method for preparing a soil conditioner of a salt-alkali tolerant bacterial agent combined with Xanthoceras sorbifolia husk according to any one of claims 1 to 4, characterized in that: The following steps are involved: Step S1, preparing salt-alkali tolerant bacterial agent fermentation liquid; Step S2, crushing the Xanthoceras sorbifolia shell into 5-200 meshes; Step S3, mixing the Xanthoceras sorbifolia shell powder and the salt-alkali-tolerant bacteria agent fermentation liquid in a ratio of 1 to 20:1 to form a preliminary mixture; Step S4, mixing the preliminary mixture, peat and humic acid in a ratio of 1:0.1-0.5:0.05-0.2 to form a mid-step mixture; Step S5, adding water and a binder to the intermediate step mixture, and making a granular soil conditioner by extrusion granulation; or air-drying or drying the intermediate step mixture to make a powdered soil conditioner.

6. The method for preparing a soil conditioner of a salt-alkali tolerant bacterial agent combined with Xanthoceras sorbifolia husk according to claim 5, characterized in that: In step S1, the method for preparing the salt-alkali tolerant bacterial agent fermentation broth comprises: Step S11, placing a carbon source, a nitrogen source, and trace elements in a fermentation system of the strain; Step S12, after high temperature sterilization and cooling to 28-32° C., inoculating one or more of Bacillus subtilis, Bacillus megaterium, Bacillus halogenus, Bacillus halogenus, Bacillus halophilus, Bacillus halophilus, Alkalimonas amyloliquefaciens, and Alkalimonas amyloliquefaciens; Step S13: After fermentation for 24 to 96 hours, a salt-alkali tolerant bacterial agent fermentation liquid is formed.

7. The method for preparing a soil conditioner of a salt-alkali tolerant bacterial agent combined with Xanthoceras sorbifolia husk according to claim 5, characterized in that: In the step S5, the binder includes diatomaceous earth.

8. The method for preparing a soil conditioner of a salt-alkali tolerant bacterial agent combined with Xanthoceras sorbifolia husk according to claim 6, characterized in that: In step S13, the ventilation volume of the fermentation is a ratio of the volume of compressed air introduced per minute to the volume of the fermentation liquid of 1:0.2-0.6, and the fermentation temperature is 28-32°C.

9. The method for preparing a soil conditioner of a salt-alkali tolerant bacterial agent combined with Xanthoceras sorbifolia husk according to claim 6, characterized in that: In step S11, the carbon source includes: glucose, fructose, maltose, sucrose, molasses, and organic acid; the nitrogen source includes: urea, ammonia, nitrate, peptone, and yeast extract; and the trace elements include: sodium, potassium, magnesium, and iron.

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