Compound enzyme soil conditioner based on basalt as carrier and preparation method of compound enzyme soil conditioner

Abstract

The invention discloses a compound enzyme soil conditioner based on basalt as a carrier and a preparation method of the compound enzyme soil conditioner, and belongs to the technical field of soil remediation. The compound enzyme soil conditioner based on basalt as the carrier is prepared from the following components in parts by weight: 500 parts of phosphate buffer, 40 to 60 parts of modified basalt carrier, 10 to 20 parts of compound enzyme, 10 to 20 parts of modified charcoal, 5 to 15 parts of compound fertilizer, 5 to 10 parts of diatomite, 2 to 5 parts of yeast extract, 1.5 to 6 parts of beneficial soil microorganisms and 1 to 2 parts of surface active substances, the modified basalt carrier is prepared from basalt through high-temperature reaction, crushing, ultrasonic treatment, acid treatment and coupling treatment; the modified biochar is prepared by treating biochar with an alkaline solution and an oxidation solution. The compound enzyme soil conditioner disclosed by the invention can be used for improving the air permeability and the water-retaining property of soil and improving the fertility of the soil.

Description

Technical Field

[0001] The invention belongs to the technical field of soil remediation, and specifically relates to a composite enzyme soil conditioner based on basalt as a carrier and a preparation method thereof. Background Art

[0002] Soil is the cornerstone of agricultural production, and its quality and health directly affect the growth and yield of crops. However, with the acceleration of industrialization and urbanization and the increasing degree of agricultural intensification, soil is facing serious pollution and degradation problems. For example, common soil pollution and degradation problems include heavy metal pollution, pesticide residues, soil compaction, decreased soil fertility, and imbalance of soil microbial communities. These challenges not only reduce the agricultural production capacity of the soil, but also pose a threat to the ecological environment and human health. Therefore, in response to the serious pollution and degradation problems facing the soil, effective measures must be taken to improve and repair the soil to ensure the sustainable development of agricultural production and protect the ecological environment and human health.

[0003] Traditional soil improvement methods, such as adding organic fertilizers and lime, can improve soil properties to a certain extent, but the effect is often limited and it is difficult to meet the comprehensive needs of modern agriculture for soil fertility, water retention capacity and microbial activity. Although some chemical improvers have significant effects in the short term, they may bring long-term problems such as environmental pollution and soil degradation.

[0004] With the continuous improvement of environmental awareness, compound enzyme soil conditioner will pay more attention to environmental protection and sustainable development. By reducing the use of chemical fertilizers and pesticides, reducing the pollution and damage to the environment caused by agricultural production, and realizing the coordinated development of agricultural production and environmental protection. Compound enzyme soil conditioner is an organic fertilizer developed using biotechnology and microbiological principles. By mixing a variety of beneficial microorganisms and enzymes and adding them to the soil, the soil structure is improved, the soil fertility is increased, and plant growth is promoted. The beneficial microorganisms in the conditioner mainly include nitrogen-fixing bacteria, phosphate-dissolving bacteria, organic matter-decomposing bacteria, etc. The microorganisms can convert nitrogen in the atmosphere into ammonia nitrogen that can be absorbed by plants, and can also convert phosphates in the soil into phosphates that can be absorbed by plants. In addition, the enzymes in the compound enzyme soil conditioner can decompose organic matter in the soil and release nutrients required by plants, thereby improving soil fertility. Therefore, the present invention develops a compound enzyme soil conditioner based on basalt as a carrier and a preparation method thereof, which is used to solve the technical problems of poor soil improvement effect and environmental pollution in the prior art that the catalytic activity of the compound enzyme is not combined with enhanced surface activity and adsorption performance. Summary of the invention

[0005] The purpose of the present invention is to provide a composite enzyme soil conditioner based on basalt as a carrier and a preparation method thereof, so as to solve the technical problems of poor soil improvement effect and environmental pollution in the prior art.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A composite enzyme soil conditioner based on basalt as a carrier is composed of the following components in parts by weight: 500 parts of phosphate buffer, 40-60 parts of modified basalt carrier, 10-20 parts of composite enzyme, 10-20 parts of modified biochar, 5-15 parts of compound fertilizer, 5-10 parts of diatomaceous earth, 2-5 parts of yeast extract, 1.5-6 parts of beneficial soil microorganisms and 1-2 parts of surfactant; the modified basalt carrier is prepared by high-temperature reaction, crushing, ultrasonic treatment, acid treatment and coupling treatment of basalt; the modified biochar is prepared by treating biochar with alkaline solution and oxidizing solution.

[0008] The pH of the phosphate buffer is 6.5-7.5; the complex enzyme is composed of the following components in parts by weight: 1-2 parts of amylase, 1-2 parts of cellulase, 1-2 parts of pectinase, 1-2 parts of hypersensitive protease, 1-2 parts of phosphatase, 1-2 parts of urease, 1-2 parts of dehydrogenase, 1-2 parts of chitinase, 1-2 parts of xylanase and 1-2 parts of superoxide dismutase; the beneficial soil microorganisms are a mixture of Bacillus licheniformis, Bacillus jelly-like, Bacillus thuringiensis, Bacillus jelly-like and Streptomyces jingyangensis, Bacillus licheniformis, Bacillus jelly-like The mass ratio of Bacillus, Bacillus thuringiensis, Bacillus subtilis and Streptomyces jingyang is 1-3:1-2:1-3:1-3:1; the surfactant is a mixture of sodium dodecyl sulfate and lecithin, and the mass ratio of sodium dodecyl sulfate to lecithin is 3-7:1; the hypersensitive protease is purchased from Qingdao Huashengrong Biotechnology Co., Ltd.; the compound fertilizer is purchased from Shandong Dijiu Biotechnology Co., Ltd.; the diatomaceous earth is purchased from Kebosheng Environmental Protection Technology Co., Ltd.; the yeast extract is purchased from Sanyuan Tianyu Biological Products Co., Ltd.

[0009] The preparation method of the modified basalt carrier comprises the following steps:

[0010] (1) Pretreatment: Put basalt into a crucible, put the crucible into a muffle furnace, and perform a high-temperature reaction. After the reaction is completed, close the muffle furnace and cool naturally to room temperature. Put the high-temperature treated basalt into a grinder and grind it, then sieve it to obtain basalt powder. Weigh the basalt powder, put it into a beaker, add deionized water, stir it evenly with a magnetic stirrer, put the beaker into an ultrasonic cleaner, perform ultrasound, and after the reaction is completed, filter it, put the filter cake into an oven to dry, and obtain pretreated basalt powder;

[0011] (2) Acid treatment: adding basalt powder to a dilute sulfuric acid solution, heating and stirring to react, filtering, washing, and drying to obtain acid-treated basalt;

[0012] (3) Coupling treatment: Weigh the acid-treated basalt and add it into a conical flask, then add anhydrous ethanol and stir evenly, then add the silane coupling agent, stir at room temperature, filter, wash, and put the filter cake into an oven to dry to obtain modified basalt.

[0013] Furthermore, in step (1), the temperature of the high temperature reaction is 600-900°C, and the temperature is raised to the set temperature at a heating rate of 5-10°C / min, and the high temperature reaction time is 2-4 hours; the grinding machine speed is 1000-3000rpm, the grinding time is 10-30 minutes, and the particle size of the basalt powder is 0.1-0.5mm; the ultrasonic power is 200-400W, the ultrasonic treatment time is 30-60 minutes, the oven drying temperature is 100-105°C, and the drying time is 10-14 hours; in step (2), the dilute sulfur The acid solution is adjusted to pH 2-3, the heating temperature is 60-80°C, the reaction is stirred for 2-4 hours, and the mixture is washed with deionized water until neutral; in step (3), the mass volume ratio of the acid-treated basalt to anhydrous ethanol is 4-5:50 g / mL, the silane coupling agent is an equal mixture of KH550 and H560, the volume ratio of the silane coupling agent to anhydrous ethanol is 2-3:50, the mixture is stirred at room temperature for 20-28 hours, the mixture is washed with anhydrous ethanol for 2-3 times, the drying temperature of the oven is 60-65°C, and the drying time is 12-18 hours.

[0014] The modified biochar comprises the following steps:

[0015] S1. Alkali treatment: weigh the biochar and put it into a beaker, add the alkaline solution, stir it evenly with a magnetic stirrer, place the beaker under heating conditions, stir and react, filter it after the reaction is completed, wash it with deionized water until it is neutral, put the filter cake into an oven to dry, and obtain the alkaline-treated biochar;

[0016] S2, oxidation treatment: weigh the alkali-treated biochar and put it into a beaker, add the oxidizing solution, stir evenly with a magnetic stirrer, place the beaker under heating conditions, stir the reaction, and after the reaction is completed, filter, wash with deionized water until neutral, put the filter cake into an oven to dry, and obtain modified biochar;

[0017] Furthermore, the biochar in S1 was purchased from Henan Miaolan Environmental Protection Technology Co., Ltd., the particle size of the biochar was 0.1-1 mm, the mass volume ratio of the biochar to the alkaline solution was 1-2:10 g / mL, the alkaline solution was one of NaOH or KOH solution, the concentration of the alkaline solution was 1-1.2 M, the heating temperature was 60-80°C, and the reaction was stirred for 2-4 hours; the mass volume ratio of the alkali-treated biochar to the oxidizing solution in S2 was 1-2:10 g / mL, the oxidizing solution was one of 3-4% H2O2 or 0.1-0.15M KMnO4 solution, the heating temperature was 60-80°C, and the reaction was stirred for 2-4 hours.

[0018] A method for preparing a composite enzyme soil conditioner based on basalt as a carrier comprises the following steps:

[0019] M1, dissolving the complex enzyme in phosphate buffer, then adding the modified basalt carrier, stirring and mixing, and then adding glutaraldehyde for cross-linking reaction to obtain a complex enzyme soil conditioner precursor solution;

[0020] M1. Add modified biochar, compound fertilizer, diatomaceous earth, yeast extract, beneficial soil microorganisms and surfactants into a compound enzyme soil conditioner precursor solution, stir evenly, and obtain a compound enzyme soil conditioner based on basalt as a carrier.

[0021] Furthermore, the concentration of glutaraldehyde in M1 is 0.2-2% (W / V), and the reaction is carried out for 2-4 hours.

[0022] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0023] 1. The present invention uses modified basalt as a composite enzyme carrier to provide more attachment sites for the composite enzyme, which not only increases the contact area between the composite enzyme and the soil, but also promotes the interaction between the composite enzyme and the substrate in the soil, thereby improving the catalytic activity of the composite enzyme; further adding modified biochar, compound fertilizer, diatomaceous earth, yeast extract, beneficial soil microorganisms and surfactants to prepare a composite enzyme soil conditioner, which overall improves the air permeability and water retention of the soil of the composite enzyme soil conditioner and promotes the growth and development of plant roots.

[0024] 2. The present invention removes moisture and volatile substances in basalt through high-temperature treatment, and at the same time, its internal structure is partially rearranged. Grinding increases its specific surface area, providing more reaction sites for subsequent treatment. Basalt is rich in trace elements such as potassium, magnesium, calcium, iron, manganese, and various minerals, which help to improve soil fertility and soil structure; acid treatment allows dilute sulfuric acid to react with components in basalt, further changing the polarity of the basalt surface and producing more microporous structures, making it easier to combine with chemical substances in subsequent treatment; in addition, the silane coupling agent reacts with the acid-treated basalt surface to form a stable chemical bond, thereby improving the catalytic activity of the complex enzyme.

[0025] 3. The present invention prepares modified biochar as a component of the composite enzyme soil conditioner, wherein an alkaline solution removes ash and impurities therein, and introduces more hydroxyl groups and oxygen-containing functional groups, thereby changing the surface properties of the biochar and improving the hydrophilicity and surface activity of the biochar. At the same time, the alkaline treatment can open the micropores and mesopores in the biochar, increase its porosity and specific surface area, and thus improve its adsorption capacity and reaction activity; further, more oxygen-containing functional groups are introduced through oxidation treatment, and the types and quantities of its surface functional groups, such as hydroxyl groups, carboxyl groups, etc., are increased, thereby further enhancing the surface activity and adsorption capacity of the biochar, making it more stable in a complex environment. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] Embodiment 1:

[0028] A composite enzyme soil conditioner based on basalt as a carrier is composed of the following components by weight: 500kg phosphate buffer, 40kg modified basalt carrier, 10kg composite enzyme, 10kg modified biochar, 5kg compound fertilizer, 5kg diatomaceous earth, 2kg yeast extract, 0.5kg Bacillus licheniformis, 0.5kg Bacillus gelatinus, 0.5kg Bacillus thuringiensis, 0.5kg Bacillus gelatinus, 0.5kg Streptomyces jingyangensis, 0.75kg sodium dodecyl sulfate and 0.25kg lecithin; the modified basalt carrier is prepared by high-temperature reaction, crushing, ultrasonic treatment, acid treatment and coupling treatment of basalt; the modified biochar is prepared by treating biochar with alkaline solution and oxidizing solution.

[0029] The pH of the phosphate buffer is 6.5; the complex enzyme is composed of the following components by weight: 1kg of amylase, 1kg of cellulase, 1kg of pectinase, 1kg of hypersensitive protease, 1kg of phosphatase, 1kg of urease, 1kg of dehydrogenase, 1kg of chitinase, 1kg of xylanase and 1kg of superoxide dismutase.

[0030] The preparation method of the modified basalt carrier comprises the following steps:

[0031] (1) Pretreatment: Put basalt into a crucible, put the crucible into a muffle furnace, and perform a high-temperature reaction. The temperature of the high-temperature reaction is 600°C, and the temperature is raised to the set temperature at a heating rate of 5°C / min. The high-temperature reaction time is 2 hours. After the reaction is completed, the muffle furnace is closed and the reaction is naturally cooled to room temperature. The high-temperature treated basalt is put into a grinder and ground at a speed of 1000 rpm for 10 minutes. Then the mixture is sieved to obtain basalt powder with a particle size of 0.1 mm. 400 g of basalt powder is weighed and put into a beaker. Deionized water is added and stirred evenly with a magnetic stirrer. The beaker is put into an ultrasonic cleaner for ultrasonic treatment. The ultrasonic power is 200 W and the ultrasonic treatment time is 30 minutes. After the reaction is completed, the filter cake is put into an oven for drying. The oven drying temperature is 100°C and the drying time is 10 hours to obtain pretreated basalt powder.

[0032] (2) Acid treatment: adding basalt powder to a dilute sulfuric acid solution, adjusting the pH of the dilute sulfuric acid solution to 2, heating and stirring the reaction, the heating temperature is 60° C., stirring and reacting for 2 hours, filtering, washing with deionized water to neutrality, and drying to obtain acid-treated basalt;

[0033] (3) Coupling treatment: Weigh the acid-treated basalt and add it into a conical flask, then add 5 L of anhydrous ethanol and stir evenly, then add 100 mL of KH550 and 100 mL of H560 silane coupling agents, stir at room temperature for 20 hours, filter, wash twice with anhydrous ethanol, put the filter cake into an oven to dry, the drying temperature of the oven is 60°C, and the drying time is 12 hours to obtain modified basalt.

[0034] The modified biochar comprises the following steps:

[0035] S1. Alkali treatment: weigh 100 g of biochar and put it into a beaker, add 1 L of 1 M NaOH solution, stir evenly with a magnetic stirrer, place the beaker under 60 ° C heating conditions, stir and react for 2 hours, filter, wash with deionized water until neutral, put the filter cake into an oven to dry, and obtain alkali-treated biochar;

[0036] S2. Oxidation treatment: weigh 100g of alkali-treated biochar and put it into a beaker, add 1L of 3% H2O2 solution, stir evenly with a magnetic stirrer, place the beaker under 60°C heating conditions, stir the reaction, and after the reaction is completed, filter, wash with deionized water until neutral, put the filter cake into an oven to dry, and obtain modified biochar.

[0037] A method for preparing a composite enzyme soil conditioner based on basalt as a carrier comprises the following steps:

[0038] M1, dissolving the complex enzyme in phosphate buffer, then adding the modified basalt carrier, stirring and mixing, and then adding 0.2% (W / V) glutaraldehyde for cross-linking reaction to obtain a complex enzyme soil conditioner precursor solution;

[0039] M1. Add modified biochar, compound fertilizer, diatomaceous earth, yeast extract, beneficial soil microorganisms and surfactants into a compound enzyme soil conditioner precursor solution, stir evenly, and obtain a compound enzyme soil conditioner based on basalt as a carrier.

[0040] Embodiment 2:

[0041] A composite enzyme soil conditioner based on basalt as a carrier is composed of the following components by weight: 500kg phosphate buffer, 50kg modified basalt carrier, 15kg composite enzyme, 15kg modified biochar, 10kg compound fertilizer, 8kg diatomaceous earth, 3kg yeast extract, 1kg Bacillus licheniformis, 1kg Bacillus gelatinus, 1kg Bacillus thuringiensis, 1kg Bacillus gelatinus, 1kg Streptomyces jingyangensis, 1.5kg sodium dodecyl sulfate and 0.5kg lecithin; the modified basalt carrier is prepared by high-temperature reaction, crushing, ultrasonic treatment, acid treatment and coupling treatment of basalt; and the modified biochar is prepared by treating biochar with alkaline solution and oxidizing solution.

[0042] The pH of the phosphate buffer is 7.2; the complex enzyme is composed of the following components by weight: 1.5kg of amylase, 1.5kg of cellulase, 1.5kg of pectinase, 1.5kg of hypersensitive protease, 1.5kg of phosphatase, 1.5kg of urease, 1.5kg of dehydrogenase, 1.5kg of chitinase, 1.5kg of xylanase and 1.5kg of superoxide dismutase.

[0043] The preparation method of the modified basalt carrier comprises the following steps:

[0044] (1) Pretreatment: Put basalt into a crucible, put the crucible into a muffle furnace, and perform a high-temperature reaction. The temperature of the high-temperature reaction is 700°C, and the temperature is raised to the set temperature at a heating rate of 7°C / min. The high-temperature reaction time is 3 hours. After the reaction is completed, the muffle furnace is closed and the reaction is naturally cooled to room temperature. The high-temperature treated basalt is put into a grinder and ground at a speed of 2000 rpm for 20 minutes. Then, the basalt powder with a particle size of 0.4 mm is obtained by sieving. 450 g of the basalt powder is weighed and put into a beaker. Deionized water is added and stirred evenly with a magnetic stirrer. The beaker is put into an ultrasonic cleaner for ultrasonic treatment. The ultrasonic power is 300 W and the ultrasonic treatment time is 50 minutes. After the reaction is completed, the filter cake is put into an oven for drying. The oven drying temperature is 0.3°C and the drying time is 12 hours to obtain the pretreated basalt powder.

[0045] (2) Acid treatment: adding basalt powder to a dilute sulfuric acid solution, adjusting the pH of the dilute sulfuric acid solution to 2.5, heating and stirring the solution, heating the solution to 70°C, stirring the solution for 3 hours, filtering the solution, washing the solution with deionized water until the solution is neutral, and drying the solution to obtain acid-treated basalt;

[0046] (3) Coupling treatment: Weigh the acid-treated basalt and add it into a conical flask, then add 5 L of anhydrous ethanol and stir evenly, then add 120 mL of KH550 and 120 mL of H560 silane coupling agents, stir at room temperature for 26 hours, filter, wash with anhydrous ethanol three times, put the filter cake into an oven to dry, the drying temperature of the oven is 62°C, and the drying time is 16 hours to obtain modified basalt.

[0047] The modified biochar comprises the following steps:

[0048] S1. Alkali treatment: weigh 150 g of biochar into a beaker, add 1 L of 1.2 M KOH solution, stir evenly with a magnetic stirrer, place the beaker under 70 ° C heating conditions, stir and react for 3 hours, filter, wash with deionized water until neutral, put the filter cake into an oven to dry, and obtain alkali-treated biochar;

[0049] S2. Oxidation treatment: weigh 150g of alkali-treated biochar into a beaker, add 1L of 0.15M KMnO4 solution, stir evenly with a magnetic stirrer, place the beaker under 70℃ heating conditions, stir the reaction, and after the reaction is completed, filter, wash with deionized water until neutral, put the filter cake into an oven to dry, and obtain modified biochar.

[0050] A method for preparing a composite enzyme soil conditioner based on basalt as a carrier comprises the following steps:

[0051] M1, dissolving the complex enzyme in phosphate buffer, then adding the modified basalt carrier, stirring and mixing, and then adding 1% (W / V) glutaraldehyde for cross-linking reaction to obtain a complex enzyme soil conditioner precursor solution;

[0052] M1. Add modified biochar, compound fertilizer, diatomaceous earth, yeast extract, beneficial soil microorganisms and surfactants into a compound enzyme soil conditioner precursor solution, stir evenly, and obtain a compound enzyme soil conditioner based on basalt as a carrier.

[0053] Embodiment 3:

[0054] A composite enzyme soil conditioner based on basalt as a carrier is composed of the following components by weight: 500kg phosphate buffer, 60kg modified basalt carrier, 20kg composite enzyme, 20kg modified biochar, 15kg compound fertilizer, 10kg diatomaceous earth, 5kg yeast extract, 1.5kg Bacillus licheniformis, 1kg gelatinous Bacillus, 1.5kg Bacillus thuringiensis, 1.5kg gelatinous Bacillus, 1kg Jingyang Streptomyces, 1.75kg sodium dodecyl sulfate and 0.25kg lecithin; the modified basalt carrier is prepared by high-temperature reaction, crushing, ultrasonic treatment, acid treatment and coupling treatment of basalt; the modified biochar is prepared by treating biochar with alkaline solution and oxidizing solution.

[0055] The pH of the phosphate buffer is 7.5; the complex enzyme is composed of the following components by weight: 2kg of amylase, 2kg of cellulase, 2kg of pectinase, 2kg of hypersensitive protease, 2kg of phosphatase, 2kg of urease, 2kg of dehydrogenase, 2kg of chitinase, 2kg of xylanase and 2kg of superoxide dismutase.

[0056] The preparation method of the modified basalt carrier comprises the following steps:

[0057] (1) Pretreatment: Put basalt into a crucible, put the crucible into a muffle furnace, and perform a high-temperature reaction. The temperature of the high-temperature reaction is 900°C, and the temperature is increased to the set temperature at a heating rate of 10°C / min. The high-temperature reaction time is 4 hours. After the reaction is completed, the muffle furnace is closed and the reaction is naturally cooled to room temperature. The high-temperature treated basalt is put into a grinder and ground at a speed of 3000 rpm for 30 minutes. Then the basalt is sieved to obtain a basalt powder with a particle size of 0.5 mm. 500 g of the basalt powder is weighed and put into a beaker. Deionized water is added and stirred evenly with a magnetic stirrer. The beaker is put into an ultrasonic cleaner for ultrasonic treatment. The ultrasonic power is 400 W and the ultrasonic treatment time is 60 minutes. After the reaction is completed, the filter cake is put into an oven for drying. The oven drying temperature is 105°C and the drying time is 14 hours to obtain a pretreated basalt powder.

[0058] (2) Acid treatment: adding basalt powder to a dilute sulfuric acid solution, adjusting the pH of the dilute sulfuric acid solution to 2-3, heating and stirring the reaction, the heating temperature is 80°C, stirring and reacting for 4 hours, filtering, washing with deionized water to neutrality, and drying to obtain acid-treated basalt;

[0059] (3) Coupling treatment: Weigh the acid-treated basalt and add it into a conical flask, then add 5 L of anhydrous ethanol and stir evenly, then add 150 mL of KH550 and 150 mL of H560 silane coupling agents, stir at room temperature for 28 hours, filter, wash with anhydrous ethanol three times, put the filter cake into an oven to dry, the drying temperature of the oven is 65°C, and the drying time is 18 hours to obtain modified basalt.

[0060] The modified biochar comprises the following steps:

[0061] S1. Alkali treatment: weigh 200 g of biochar into a beaker, add 1 L of 1.2 M NaOH solution, stir evenly with a magnetic stirrer, place the beaker under 80 ° C heating conditions, stir and react for 4 hours, filter, wash with deionized water until neutral, put the filter cake into an oven to dry, and obtain alkali-treated biochar;

[0062] S2. Oxidation treatment: weigh 200g of alkali-treated biochar and put it into a beaker, add 1L of 4% H2O2 solution, stir evenly with a magnetic stirrer, place the beaker under 80°C heating conditions, stir the reaction, and after the reaction is completed, filter, wash with deionized water until neutral, put the filter cake into an oven to dry, and obtain modified biochar.

[0063] A method for preparing a composite enzyme soil conditioner based on basalt as a carrier comprises the following steps:

[0064] M1, dissolving the complex enzyme in phosphate buffer, then adding the modified basalt carrier, stirring and mixing, and then adding 2% (W / V) glutaraldehyde for cross-linking reaction to obtain a complex enzyme soil conditioner precursor solution;

[0065] M1. Add modified biochar, compound fertilizer, diatomaceous earth, yeast extract, beneficial soil microorganisms and surfactants into a compound enzyme soil conditioner precursor solution, stir evenly, and obtain a compound enzyme soil conditioner based on basalt as a carrier.

[0066] Comparative Example 1:

[0067] Comparative Example 1 Compared with Example 3, in the preparation process of the complex enzyme soil conditioner in Comparative Example 1, the modified basalt carrier is replaced with basalt, and other conditions remain unchanged.

[0068] Comparative Example 2:

[0069] Comparative Example 2 Compared with Example 3, in the preparation process of the complex enzyme soil conditioner in Comparative Example 2, the modified biochar was replaced with biochar, and other conditions remained unchanged.

[0070] Comparative Example 3:

[0071] Comparative Example 3 Compared with Example 3, in the preparation process of the complex enzyme soil conditioner in Comparative Example 3, no beneficial soil microorganisms were added, and other conditions remained unchanged.

[0072] Experimental example:

[0073] The compound enzyme soil conditioner prepared by Examples 1-3 and Comparative Examples 1-3 was tested in the sandy area of ​​Gonghe Basin in Qinghai Province. The test lasted for 90 days. Soil with consistent level was selected. The compound enzyme soil conditioner was applied once every 30 days at a rate of 40 kg / mu. The soil without the compound enzyme soil conditioner was used as a blank control. On the 90th day, samples were taken for the following experimental determinations:

[0074] 1. Soil Porosity

[0075] The porosity of the soil after applying the compound enzyme soil conditioner was measured according to the pycnometer method in GB / T 18877-2002 soil specific volume determination method. The results are shown in Table 1:

[0076] Table 1

[0077] Group Porosity(%) Example 1 46 Example 2 48 Example 3 49 Comparative Example 1 65 Comparative Example 2 66 Comparative Example 3 68 Control group 72

[0078] According to the test results in Table 1, the compound enzyme soil conditioner prepared by Examples 1-3 of the present invention significantly improved the porosity of the soil, but was not less than 40%. While ensuring good air permeability and water permeability of the soil, it also ensured good stability and did not cause the soil to be too loose. By comparing Comparative Examples 1-3 with Examples 1-3, it can be seen that adding modified basalt carriers, modified biochar and beneficial soil microorganisms can improve the air permeability and water permeability of the soil.

[0079] 2. Determination of soil moisture retention capacity

[0080] The soil moisture content after applying the compound enzyme soil conditioner was measured according to the soil moisture determination method of GB / T 14555-2017. The results are shown in Table 1:

[0081] Table 2

[0082] Group Field water holding capacity (%) Example 1 22 Example 2 23 Example 3 26 Comparative Example 1 11 Comparative Example 2 10 Comparative Example 3 13 Control group 7

[0083] According to the test results in Table 2, the compound enzyme soil conditioner prepared in Examples 1-3 of the present invention significantly improves the field water holding capacity. By comparing Comparative Examples 1-3 with Examples 1-3, it can be seen that adding modified basalt carriers, modified biochar and beneficial soil microorganisms can improve the water retention capacity of the soil.

[0084] 3. Determination of soil nutrient content

[0085] According to the Kjeldahl method in the NY / T 883-2004 soil total nitrogen determination method, the molybdenum antimony countercolorimetric method in the NY / T 884-2004 soil available phosphorus determination method, the flame photometry in the NY / T 885-2004 soil available potassium determination method and the colorimetric method in the NY / T 1377-2007 soil pH determination method, the results of the determination are shown in Table 1:

[0086] Table 3

[0087] Group Total nitrogen content (%) Effective phosphorus content (%) Effective potassium content (%) pH Example 1 0.26 16 112 7.2 Example 2 0.28 18 118 7.1 Example 3 0.32 20 125 7.0 Comparative Example 1 0.12 10 82 7.9 Comparative Example 2 0.18 11 87 7.7 Comparative Example 3 0.19 12 90 7.9 Control group 0.06 5.1 52 8.3

[0088] According to the test results in Table 3, the compound enzyme soil conditioner prepared in Examples 1-3 of the present invention significantly improved the nutrient content of the soil. By comparing Comparative Examples 1-3 with Examples 1-3, it can be seen that adding modified basalt carriers, modified biochar and beneficial soil microorganisms can improve soil fertility.

[0089] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

[0090] The preferred embodiments of the present invention disclosed above are only used to help explain the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to only specific implementation methods. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A composite enzyme soil conditioner based on basalt as a carrier, characterized in that: The invention is composed of the following components in parts by weight: 500 parts of phosphate buffer, 40-60 parts of modified basalt carrier, 10-20 parts of complex enzyme, 10-20 parts of modified biochar, 5-15 parts of compound fertilizer, 5-10 parts of diatomaceous earth, 2-5 parts of yeast extract, 1.5-6 parts of beneficial soil microorganisms and 1-2 parts of surfactant; the modified basalt carrier is prepared by subjecting basalt to high-temperature reaction, crushing, ultrasonic treatment, acid treatment and coupling treatment; the modified biochar is prepared by subjecting biochar to alkaline solution and oxidizing solution treatment.

2. The composite enzyme soil conditioner based on basalt as a carrier according to claim 1, characterized in that: The pH value of the phosphate buffer is 6.5-7.5; the complex enzyme is composed of the following components in parts by weight: 1-2 parts of amylase, 1-2 parts of cellulase, 1-2 parts of pectinase, 1-2 parts of hypersensitive protease, 1-2 parts of phosphatase, 1-2 parts of urease, 1-2 parts of dehydrogenase, 1-2 parts of chitinase, 1-2 parts of xylanase and 1-2 parts of superoxide dismutase; the beneficial soil microorganisms are a mixture of Bacillus licheniformis, Bacillus gelatinous, Bacillus thuringiensis, Bacillus gelatinous and Streptomyces jingyangensis, and the mass ratio of Bacillus licheniformis, Bacillus gelatinous, Bacillus thuringiensis, Bacillus gelatinous and Streptomyces jingyangensis is 1-3:1-2:1-3:1-3:1; the surfactant is a mixture of sodium dodecyl sulfate and lecithin, and the mass ratio of sodium dodecyl sulfate to lecithin is 3-7:

1.

3. The composite enzyme soil conditioner based on basalt as a carrier according to claim 1, characterized in that: The preparation method of the modified basalt carrier comprises the following steps: (1) Pretreatment: Put basalt into a crucible, put the crucible into a muffle furnace, and perform a high-temperature reaction. After the reaction is completed, close the muffle furnace and cool naturally to room temperature. Put the high-temperature treated basalt into a grinder and grind it, then sieve it to obtain basalt powder. Weigh the basalt powder, put it into a beaker, add deionized water, stir it evenly with a magnetic stirrer, put the beaker into an ultrasonic cleaner, perform ultrasound, and after the reaction is completed, filter it, put the filter cake into an oven to dry, and obtain pretreated basalt powder; (2) Acid treatment: adding basalt powder to a dilute sulfuric acid solution, heating and stirring to react, filtering, washing, and drying to obtain acid-treated basalt; (3) Coupling treatment: Weigh the acid-treated basalt and add it into a conical flask, then add anhydrous ethanol and stir evenly, then add the silane coupling agent, stir at room temperature, filter, wash, and put the filter cake into an oven to dry to obtain modified basalt.

4. The composite enzyme soil conditioner based on basalt as a carrier according to claim 3, characterized in that: In step (1), the temperature of the high temperature reaction is 600-900° C., and the temperature is raised to the set temperature at a heating rate of 5-10° C. / min. The high temperature reaction time is 2-4 hours; the grinding machine speed is 1000-3000 rpm, the grinding time is 10-30 minutes, and the particle size of the basalt powder is 0.1-0.5 mm; the ultrasonic power is 200-400 W, the ultrasonic treatment time is 30-60 minutes, the oven drying temperature is 100-105° C., and the drying time is 10-14 hours.

5. The composite enzyme soil conditioner based on basalt as a carrier according to claim 3, characterized in that: In step (2), the pH value of the dilute sulfuric acid solution is adjusted to 2-3, the heating temperature is 60-80° C., the reaction is stirred for 2-4 hours, and the mixture is washed with deionized water until neutral; in step (3), the mass volume ratio of the acid-treated basalt to anhydrous ethanol is 4-5:50 g / mL, the silane coupling agent is an equal mixture of KH550 and H560, the volume ratio of the silane coupling agent to anhydrous ethanol is 2-3:50, the mixture is stirred at room temperature for 20-28 hours, the mixture is washed with anhydrous ethanol for 2-3 times, the drying temperature of the oven is 60-65° C., and the drying time is 12-18 hours.

6. The composite enzyme soil conditioner based on basalt as a carrier according to claim 1, characterized in that: The modified biochar comprises the following steps: S1. Alkali treatment: weigh the biochar and put it into a beaker, add the alkaline solution, stir it evenly with a magnetic stirrer, place the beaker under heating conditions, stir and react, filter it after the reaction is completed, wash it with deionized water until it is neutral, put the filter cake into an oven to dry, and obtain the alkaline-treated biochar; S2. Oxidation treatment: weigh the alkali-treated biochar and put it into a beaker, add the oxidizing solution, stir evenly with a magnetic stirrer, place the beaker under heating conditions, stir the reaction, and after the reaction is completed, filter it, wash it with deionized water until it is neutral, put the filter cake into an oven to dry, and obtain modified biochar.

7. The composite enzyme soil conditioner based on basalt as a carrier according to claim 6, characterized in that: The particle size of the biochar in S1 is 0.1-1 mm, the mass volume ratio of the biochar to the alkaline solution is 1-2:10 g / mL, the alkaline solution is one of NaOH or KOH solution, the concentration of the alkaline solution is 1-1.2 M, the heating temperature is 60-80° C., and the reaction is stirred for 2-4 hours.

8. The composite enzyme soil conditioner based on basalt as a carrier according to claim 6, characterized in that: The mass volume ratio of the alkali-treated biochar to the oxidizing solution in S2 is 1-2:10 g / mL, the oxidizing solution is one of 3-4% H2O2 or 0.1-0.15M KMnO4 solution, the heating temperature is 60-80°C, and the reaction is stirred for 2-4 hours.

9. A method for preparing a composite enzyme soil conditioner based on basalt as a carrier, characterized in that: The following steps are involved: M1, dissolving the complex enzyme in phosphate buffer, then adding the modified basalt carrier, stirring and mixing, and then adding glutaraldehyde for cross-linking reaction to obtain a complex enzyme soil conditioner precursor solution; M1. Modify biochar 、 Compound Fertilizer 、 diatomite 、 Yeast Extract 、 Beneficial soil microorganisms and surfactants are added into a compound enzyme soil conditioner precursor solution and stirred evenly to obtain a compound enzyme soil conditioner based on basalt as a carrier.

10. The method for preparing a composite enzyme soil conditioner based on basalt as a carrier according to claim 9, characterized in that: The concentration of glutaraldehyde in M1 is 0.2-2% (W / V), and the reaction is carried out for 2-4 hours.