A whitefly attractant and its use in agricultural production

Abstract

The application discloses a whitefly attractant and application thereof in agricultural production and belongs to the technical field of plant protection. The whitefly attractant is characterized in that effective components are two components of octaethylene glycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol which are used alone or mixed, and the concentration of the effective components is 0.5-62.5 ug / uL. Through indoor and field tests on the attraction effect of the single or mixed components, it is found that the whitefly has a good attraction effect, is targeted, has a good control effect, and has the characteristics of simple, convenient operation, a wide effective concentration range, a high fault tolerance and a high attraction efficiency, and the octaethylene glycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol are less toxic to humans and mammals and friendly to the environment. The application has important application value in the green and efficient control technology of the whitefly and has wide economic, social and ecological benefits.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant protection, in particular, the present application relates to a kind of whitefly attractant and its application in agricultural production. BACKGROUND

[0002] Bemisia tabaci (Gennadius) belongs to Hemiptera, Aleyrodidae, Stenorrhyncha, is a kind of world-wide outbreak serious super agricultural pest in horticultural flowers and economic crops, and there are more than 600 known host plants. Mainly through feeding plant sap, transmitting a variety of plant viruses, secreting a large amount of honeydew, inducing soot blight, causing significant economic losses to crops. At present, the conventional whitefly control method mostly depends on the use of chemical pesticides, but long-term single use of chemical pesticides, not only can insects produce drug resistance, and pesticide residues and other problems also cause environmental pollution. Therefore, using plant-derived compounds to develop new control agents has become the research focus of new plant protection technology. Can further reduce the use of chemical pesticides, not only delays the generation of pest resistance, but also solves the problems of environmental pollution, pesticide residues and other problems. Therefore, developing "green plant protection prevention and control technology" strategy has become the primary problem in the field of new plant protection technology research. SUMMARY

[0003] Based on this, the present application provides a kind of whitefly attractant and its application in agricultural production. Specifically, the effective component of the whitefly attractant provided by the present application is at least one of octyl vinyl glycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol.

[0004] When the effective component of the attractant of the present application is octyl vinyl glycol monododecyl ester, the preferred effective component concentration is 0.5ug / uL-12.5ug / uL.

[0005] When the effective component of the attractant of the present application is 3,6,9,12-tetraoxatetradecan-1-ol, the preferred effective component concentration is 12.5ug / uL-62.5ug / uL.

[0006] When the effective component of the attractant of the present application is octyl vinyl glycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol, the preferred effective component concentration is 0.5ug / uL-62.5ug / uL.

[0007] When the effective component of the attractant of the present application is octyl vinyl glycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol, the best effective component concentration is 12.5ug / uL.

[0008] When the effective components of the attractant of the present application are octaethylene glycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol, the volume ratio of octaethylene glycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol at the same concentration is preferably 6:4-9:1 or 1:9-2:8.

[0009] When the effective components of the attractant of the present application are octaethylene glycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol, the volume ratio of octaethylene glycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol at the same concentration is preferably 6:4-9:1 or 1:9-2:8.

[0010] The present application also relates to the application of the whitefly attractant in agricultural production. The attractant is mixed with a carrier or a solvent and hung in a field where the whitefly is harmful. Preferably, the time of hanging in the field where the whitefly is harmful is 1-17 days, and the optimal time of hanging in the field where the whitefly is harmful is 1-9 days.

[0011] The whitefly attractant provided by the present application has good attracting effect on the whitefly, is targeted, has good control effect, and has the characteristics of simple operation, convenience, wide effective concentration range, high fault tolerance and high attracting efficiency. In addition, octaethylene glycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol are less toxic to humans and mammals and are environmentally friendly. The present application has important application value in the green and efficient control technology of the whitefly and has wide economic, social and ecological benefits. DETAILED DESCRIPTION

[0012] The present application will be further described below by examples. It should be understood that the methods described in the examples of the present application are only used to illustrate the present application, but not to limit the present application. Simple improvements of the present application under the concept of the present application belong to the scope of protection of the present application.

[0013] 1. Test materials

[0014] Octaethylene glycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol are conventional commercially available goods; whitefly adults: the whitefly MED cryptic species was first collected from Euphorbia pulcherrima plants in a flower market in Beijing in 2009. After the biological type was identified in the laboratory, the whitefly was long-term reared on healthy and pest-free cotton (Jingmian 11) plants without spraying pesticides. The whitefly rearing greenhouse conditions were as follows: the temperature was set to 25℃±1℃, the humidity was set to 70±5%, and the light cycle was set to 14:10 (light:dark).

[0015] 2. Test method one

[0016] Octyl vinyl alcohol monododecyl ester: (CAS No: 3055-98-9), 3,6,9,12- tetraoxatetradecan-1-ol (CAS No: 5650-26-4) were used as active components, each compound was first configured into a 1 μg / uL stock solution with ethanol, and then diluted with double distilled water into 0.1 μg / uL, 0.5 μg / uL, 2.5 μg / uL, 12.5 μg / uL and 62.5 μg / uL. We compared the behavioral responses of female and male whitefly to volatile substances with the control group by insect "Y" olfactometer. In the "Y" olfactometer selection test, the olfactometer device is composed of a glass tube with a diameter of 3.5 cm, which has a base and two arms with a length of 15 cm, and each arm has an angle of 60° between them. Each arm is connected to a rubber hose leading to the odor source. The lid of each flask is equipped with an air inlet tube connected to an air pump and an air outlet tube connected to the olfactometer arm. The charcoal-filtered air is pushed into the olfactometer arm by a vacuum pressure pump, and the air flow is maintained at 100 mL min -1 .

[0017] Before the behavioral selection test, newly emerged and unmated whitefly adults within 24 hours were selected, and the adults were starved for 4 hours before the test and were selected separately by gender. 200 ul of volatile solution was evenly dropped on a filter paper with a size of 3 cm*1 cm, and was placed in the odor source bottle as the odor source, and water was used as the control. Before the Y-tube test, the air was ventilated for 10 min to make the odor fill the pipeline. 10 whitefly adults were introduced into the main arm of the Y-olfactometer, and the test time was 5 min. When the whitefly adults crawled more than two-thirds of the olfactometer arm and remained for at least 15 seconds, it was considered that the whitefly selected this odor source. If the adults did not make a choice within 5 minutes, it was recorded as "no choice". After testing 10 whitefly adults, the position of the Y-tube and the odor source were changed, and after the test of each sample was completed, all Y-tubes and Teflon tubes were cleaned with absolute ethanol and dried to ensure that the odor of the residual sample solution in the tube was completely removed. The next sample test was performed. Each test was repeated 10 times, the selection rate was calculated, the selection rate calculation formula = (number of insects in the odor source tube) / (number of insects in the odor source tube + number of insects in the control tube) x 100, and the statistical results were subjected to chi-square test. All tests were conducted at 26-28°C from 9:00 to 17:00.

[0018] The statistical analysis results are shown in Table 1, and the results show that, relative to CK, different concentrations of octenylene glycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol have a significant influence on the selection behavior of B. tabaci (Table 1). The number of B. tabaci females or males attracted by 3,6,9,12-tetraoxatetradecan-1-ol is less at low concentrations (0.1 μg / uL-2.5 μg / uL), while the female and male B. tabaci adults have a significant attraction to 3,6,9,12-tetraoxatetradecan-1-ol at high concentrations (12.5 μg / uL, 62.5 μg / uL) (χ 2 = 32.584, P = 0.000; χ 2 = 7.685, P = 0.006; χ 2 = 27.348, P < 0.0000; χ 2 = 8.628, P = 0.003), and there is also a significant difference between females and males (χ 2 = 10.080, P = 0.001; χ 2 = 6.190, P = 0.013).

[0019] 0.1 μg / uL of octenylene glycol monododecyl ester has no significant attraction to B. tabaci female and male adults. 0.5 μg / uL, 2.5 μg / uL and 12.5 μg / uL of octenylene glycol monododecyl ester have a significant attraction to B. tabaci female adults (χ 2 = 4.123, P = 0.042; χ 2 = 6.510, P = 0.011; χ 2 = 4.456, P = 0.035), and the males show a similar selection trend. However, 62.5 μg / mL of octenylene glycol monododecyl ester has no significant attraction to B. tabaci adults, and there is no significant difference between the responses of females and males (χ 2 = 1.140, P = 0.286) (Table 1).

[0020] Table 1 Selection trend of B. tabaci to different attractants

[0021]

[0022] The above test results show that, for 3,6,9,12-tetraoxatetradecan-1-ol alone, the optimal concentration is 12.5 μg / uL-62.5 μg / uL. For octenylene glycol monododecyl ester, the optimal concentration is 0.5 μg / uL-12.5 μg / uL.

[0023] 3. Test method two

[0024] Field Bemisia tabaci attraction test of octaethylene glycol mono-n-dodecyl ester, 3,6,9,12-tetraoxatetradecan-1-ol. Octaethylene glycol mono-n-dodecyl ester, 3,6,9,12-tetraoxatetradecan-1-ol two compounds were respectively prepared into a concentration of 12.5 μg / uL, loaded into a 1.5 mL centrifuge tube, open the centrifuge tube cover, placed in the insect trap, a white sticky insect board was hung below the trap, the trap was hung in the cotton field at a position 15-20 cm above the cotton plant, the Bemisia tabaci attraction test was carried out, the control area was placed in the centrifuge tube with water, octaethylene glycol mono-n-dodecyl ester, 3,6,9,12-tetraoxatetradecan-1-ol and water control were randomly placed, the distance between each treatment was 4-5 meters, each treatment was repeated 6 times, the attraction results were counted respectively after hanging for 1 day, 9 days and 17 days, a total of 3 times of investigation, a new centrifuge tube and its white sticky insect board were replaced at each time of investigation, and the statistical results were subjected to one-way ANOVA statistical analysis.

[0025] The results showed that after hanging for 1 day and 9 days, octaethylene glycol mono-n-dodecyl ester, 3,6,9,12-tetraoxatetradecan-1-ol had a significant attraction effect on Bemisia tabaci (P=0.005 and P=0.001), after hanging for 17 days, the number of insects attracted in the two compound treatment groups was higher than that in the CK group, but the difference between the three was not significant (P=0.1) (Table 2).

[0026] Table 2 Field selection test of Bemisia tabaci to different attractants

[0027]

[0028] 4. Test method three

[0029] According to the results of Experiment One, a mixing test of octaethylene glycol mono-n-dodecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol was carried out. Octaethylene glycol mono-n-dodecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol were respectively prepared into a concentration of 12.5 μg / uL, then octaethylene glycol mono-n-dodecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol were mixed according to the volume ratio of 9:1, 8:2, 7:3, 6:4, 5:5; 4:6, 3:7, 2:8, 1:9, a total of 9 kinds of mixed formulations. The Y-type olfactometer was used for Bemisia tabaci adult selection test, and the specific test operation method was the same as "Experiment Method One".

[0030] The results show that the selective response of Bemisia tabaci to the complex preparation of octylene glycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol is not regular with different concentration ratios, but from Table 3, it can be seen that the preparation of different ratios of the two volatile substances has a significant attraction effect on Bemisia tabaci adults. Among them, octylene glycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol at the ratio of 9:1 have the most significant attraction effect on Bemisia tabaci adults (P=0.000), and at the ratios of 5:5, 4:6 and 3:7, although they also have an attraction effect on Bemisia tabaci, the difference is not significant (P=0.303-0.394). Overall, octylene glycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol at different concentration ratios have an attraction effect on Bemisia tabaci adults.

[0031] Table 3 Selective trend of Bemisia tabaci to different complex attractants

[0032]

[0033] The above examples only express several embodiments of the present application, and the description is more specific and detailed, but it should not be understood as a limitation on the scope of the patent of the present application. It should be noted that for ordinary skilled in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which are all within the scope of protection of the present application. Therefore, the protection scope of the patent of the present application should be subject to the appended claims.

Claims

1. Use of octaethyleneglycol monododecyl ester and / or 3,6,9,12-tetraoxatetradecan-1-ol as an attractant for whitefly.

2. Use according to claim 1, characterized in that, When the effective component of the attractant is octaethyleneglycol monododecyl ester, the effective component concentration is 0.5 ug / uL-12.5 ug / uL.

3. Use according to claim 1, characterized in that, When the effective component of the attractant is 3,6,9,12-tetraoxatetradecan-1-ol, the effective component concentration is 12.5 ug / uL-62.5 ug / uL.

4. Use according to claim 1, characterized in that, When the effective component of the attractant is octaethyleneglycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol, the effective component concentration is 0.5 ug / uL-62.5 ug / uL.

5. Use according to claim 4, characterized in that, When the effective component of the attractant is octaethyleneglycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol, the effective component concentration is 12.5 ug / uL.

6. Use according to claim 1, characterized in that, When the effective component of the attractant is octaethyleneglycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol, the same concentration volume ratio of octaethyleneglycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol is 6:4-9:1 or 1:9-2:

8.

7. Use according to claim 6, characterized in that, When the effective component of the attractant is octaethyleneglycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol, the same concentration volume ratio of octaethyleneglycol monododecyl ester and 3,6,9,12-tetraoxatetradecan-1-ol is 9:

1.

8. Use according to any one of claims 1 to 7, characterized in that, The attractant is mixed with a carrier or solvent and hung in the field infested by whitefly.

9. Use according to claim 8, characterized in that, The time of hanging the attractant in the field infested by whitefly is 1-17 days.

10. Use according to claim 9, characterized in that, The time of hanging the attractant in the field infested by whitefly is 1-9 days.

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