Piglet food calling composition containing protein hydrolysate and preparation method of piglet food calling composition

Abstract

The invention discloses a piglet food calling composition containing protein hydrolysate and a preparation method of the piglet food calling composition, and belongs to the technical field of feed. The invention discloses a piglet food calling composition. Comprising the following components in parts by weight: 15 to 25 parts of corn starch, 15 to 22 parts of modified protein zymolyte, 8 to 12 parts of lactose, 4 to 6 parts of modified yeast cell wall polysaccharide, 2 to 3 parts of fish oil, 0.8 to 1.2 parts of an acidifying agent, 0.4 to 0.6 part of sodium chloride, 0.6 to 0.8 part of a garlic extract, 0.1 to 0.3 part of a mint extract, 0.2 to 0.5 part of calcium carbonate, 0.1 to 0.3 part of calcium hydrophosphate, 0.2 to 0.4 part of cane sugar, 0.05 to 0.2 part of a milk flavoring agent and 0.05 to 0.2 part of an immunopotentiator. The prepared piglet food calling composition can improve the growth performance of piglets.

Description

Technical Field

[0001] The invention belongs to the technical field of feed, and particularly relates to a piglet feeding attractant composition containing protein hydrolysate and a preparation method thereof. Background Art

[0002] Piglets have unique physiological characteristics and nutritional needs during their growth and development. The digestive system of newly weaned piglets is not fully developed, gastric acid secretion is insufficient, intestinal villi are short and underdeveloped, and digestive enzyme activity is low. This makes them less able to digest and absorb feed, and it is difficult for them to adapt to the transition from breast milk to solid feed. At the same time, piglets grow fast and have an urgent need for nutrients. They need high-quality nutrients such as protein, energy, vitamins and minerals to support their rapid growth and development. For example, protein is a key nutrient for piglet growth. It is involved in the construction and repair of body tissues, but due to the imperfection of the piglet's digestive enzyme system, the ability to digest and absorb protein in conventional feed is limited, which requires special nutritional strategies to meet their protein needs.

[0003] In the piglet breeding process, food inducing is a key link. Since weaned piglets face stress from many aspects such as environment, feed and psychology, they often have problems such as poor appetite and low feed intake. This will not only affect the growth rate and weight gain of piglets, but also may cause the piglets' immunity to decline, making them susceptible to diseases, increasing breeding costs and mortality. A good food inducing effect can increase the piglets' feed intake, promote their growth and development, reduce stress reactions, and improve breeding benefits. For example, by increasing the piglets' feed intake, piglets can take in enough nutrients, enhance their physique, improve their resistance to diseases, and thus reduce losses in the breeding process. Therefore, the present invention develops a piglet food inducing composition containing proteolytic enzymes and a preparation method thereof, which are used to solve the technical problems that the flavor and taste of piglet feed in the prior art cannot effectively attract piglets, resulting in low food intake and low feed digestibility caused by the imperfect development of the piglet digestive system. Summary of the invention

[0004] The purpose of the present invention is to provide a piglet feeding attractant composition containing protein hydrolysate and a preparation method thereof, so as to solve the technical problems in the prior art that the flavor and taste of piglet feed cannot effectively attract piglets, resulting in low food intake and low feed digestibility caused by the imperfect development of the piglet digestive system.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] The piglet feeding attractant composition containing protein hydrolysate comprises the following components in parts by weight: 15-25 parts of corn starch, 15-22 parts of modified protein hydrolysate, 8-12 parts of lactose, 4-6 parts of modified yeast cell wall polysaccharide, 2-3 parts of fish oil, 0.8-1.2 parts of acidulant, 0.4-0.6 parts of sodium chloride, 0.6-0.8 parts of garlic extract, 0.1-0.3 parts of peppermint extract, 0.2-0.5 parts of calcium carbonate, 0.1-0.3 parts of calcium hydrogen phosphate, 0. 2-0.4 parts of sucrose, 0.05-0.2 parts of milk flavoring agent and 0.05-0.2 parts of immunopotentiator; the acidulant is an equal mixture of citric acid and fumaric acid; the immunopotentiator is obtained by mixing astragalus polysaccharide and beta-glucan in a mass ratio of 3:2; the modified protein hydrolysate is obtained by enzymatic hydrolysis and modification of soybean meal, meat and bone meal and cottonseed meal; the modified yeast cell wall polysaccharide is obtained by sulfonation modification of yeast cell wall polysaccharide and then compounding it with soybean lecithin and cholesterol.

[0007] Furthermore, the method for preparing the modified protein hydrolysate comprises the following steps:

[0008] S1. Grind soybean meal, meat and bone meal and cottonseed meal respectively, sieve, mix evenly according to proportion, add deionized water to adjust the slurry to obtain a substrate solution; adjust the pH of the substrate solution, preheat, add complex enzyme, stir evenly, and perform enzymolysis to obtain an enzymolysis solution;

[0009] S2, adding glucose to the enzymatic hydrolysate, heating for reaction, obtaining a composite solution, treating with ultra-high pressure, adding probiotics for fermentation after cooling, obtaining a fermentation liquid, filtering, and low-temperature vacuum drying to obtain a modified enzymatic hydrolysate.

[0010] Further, S1 is sieved with 60-80 mesh, soybean meal, meat and bone meal and cottonseed meal are mixed in a mass ratio of 5:3:3, the solid-liquid ratio of slurry adjustment is 1:8-12, the pH value is adjusted to 7-8 with 1 mol / L hydrochloric acid or sodium hydroxide solution, the preheating temperature is 50-55°C, the composite enzyme is a neutral protease, an alkaline protease, a cellulase and a lipase mixed in a ratio of 3-4:2:1:1, the mass ratio of the composite enzyme to the substrate is 1.5-2:100, the enzymatic reaction time is 4-6h, and stirring is performed every 30 minutes.

[0011] Furthermore, the mass ratio of glucose to enzymatic hydrolysate in S2 is 3-5:100, the heating temperature is 90-100°C, the reaction time is 1-2h, the ultra-high pressure treatment pressure is 300-400MPa, the treatment time is 10-20min, the mass ratio of probiotics to enzymatic hydrolysate is 4-6:100, the probiotics are prepared from lactic acid bacteria and Bacillus according to 2:1-2, the fermentation temperature is 36-38°C, the fermentation time is 30-48h, and the fermentation is carried out under anaerobic conditions.

[0012] Furthermore, the method for preparing the modified yeast cell wall polysaccharide comprises the following steps:

[0013] Q1. Ultrafinely grind yeast cell wall polysaccharide to obtain yeast cell wall polysaccharide powder, weigh the yeast cell wall polysaccharide powder and disperse it with anhydrous ethanol, drop pyridine, stir, heat, drop chlorosulfonic acid, stir while dropping, continue to react after the drop addition is completed, and after the reaction is completed, obtain a reaction solution, cool it to room temperature, pour the reaction solution into ice water to quench the reaction, centrifuge, take the precipitate, wash, and dry to obtain sulfonated yeast cell wall polysaccharide;

[0014] Q2. Weigh soybean lecithin and cholesterol, dissolve them in a chloroform-methanol mixed solution to obtain a lipid solution, weigh sulfonated yeast cell wall polysaccharide, dissolve them in a phosphate buffer to obtain a sulfonated yeast cell wall polysaccharide solution, add the solution dropwise to the lipid solution, stir while adding, heat, and rotary evaporate to obtain a lipid film, add a phosphate buffer to hydrate, perform ultrasonic treatment to obtain an emulsion, dialyze, and freeze-dry to obtain a modified yeast cell wall polysaccharide.

[0015] Furthermore, the particle size of Q1 is 5-10 microns, the mass volume ratio of yeast cell wall polysaccharide powder to anhydrous ethanol is 1:10-15 g / mL, the mass ratio of yeast cell wall polysaccharide powder to pyridine is 1:0.05-0.1, the stirring speed is 200-300 r / min, the heating temperature is 40-50°C, the mass ratio of chlorosulfonic acid to yeast cell wall polysaccharide is 4-10:100, the dropping time is 30-60 min, the continued reaction time is 3-4 h, the volume ratio of the reaction solution to ice water is 1:3-5, the centrifugal speed is 4000-5000 r / min, the centrifugal time is 10-15 min, the washing is done with deionized water until the pH value of the washing solution is 6.5-7.5, and the drying temperature is 50-60°C.

[0016] Furthermore, the ratio of soybean lecithin, cholesterol and chloroform-methanol mixed solution in Q2 is 100-200 mg: 20-50 mg: 10-20 mL, the chloroform-methanol mixed solution is obtained by mixing chloroform and methanol in a volume ratio of 2: 1, the ratio of sulfonated yeast cell wall polysaccharide to phosphate buffer is 20-50 mg: 10-20 mL, the volume ratio of lipid solution to sulfonated yeast cell wall polysaccharide solution is 3-8: 1, and the stirring speed is 150-25 0r / min, heating temperature is 40-50℃, rotary evaporation vacuum is 0.08-0.09MPa, rotary evaporation time is 30-60min, hydration time is 1-2h, ultrasonic power is 200-300W, ultrasonic time is 10-15min, ultrasound is intermittent, ultrasound is 10 seconds, interval is 5 seconds, the molecular weight cutoff of the dialysis bag is 8000-14000Da, dialysis time is 24-48 hours, during which the dialysate is replaced every 4-6 hours.

[0017] The preparation method of a piglet feeding attractant composition containing protein hydrolysate comprises the following steps:

[0018] (1) corn starch, modified protein hydrolysate, modified yeast cell wall polysaccharide and calcium carbonate are crushed and sieved respectively; then milk flavoring agent, sucrose and part of corn starch are preliminarily stirred and mixed, then acidulant, sodium chloride, garlic extract, mint extract and immunopotentiator are added, and stirring is continued to obtain a preliminary mixture;

[0019] (2) Add the remaining corn starch, modified protein hydrolysate, lactose, and modified yeast cell wall polysaccharide to the preliminary mixture, stir to obtain a solid mixture, spray fish oil on the surface of the solid mixture, stir, and finally add calcium carbonate and calcium hydrogen phosphate to obtain a piglet feeding attractant composition containing protein hydrolysate.

[0020] Furthermore, in step (1), the corn starch is sieved through an 80-100 mesh sieve, the portion of corn starch is 10-20% of the total mass of corn starch, the stirring speed is 30-50 r / min, and the stirring time is 5-10 min; in step (2), the stirring speed is 50-60 r / min, and the stirring time is 15-20 min.

[0021] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0022] 1. The components in the composition of the present invention work synergistically. In addition to meeting the nutritional needs of the growth and development of piglets, the composition also adds garlic extract, mint extract, milk flavoring agent, sucrose, and special flavors brought by modified protein hydrolysate and Maillard reaction products, which can effectively stimulate the sense of smell and taste of piglets and increase the feed intake of piglets.

[0023] 2. When preparing the modified protein hydrolysate, the present invention hydrolyzes soybean meal, meat and bone meal and cottonseed meal, and ferments them with lactic acid bacteria and bacillus. Probiotics can colonize in the intestines of piglets, inhibit the growth of harmful bacteria, regulate the balance of intestinal microecology, enhance the intestinal health of piglets, and improve immunity and disease resistance. In addition, ultra-high pressure treatment can change the structure of protein, making it easier to be acted upon by digestive enzymes, further improving the digestibility and bioavailability of protein, adding glucose for heating reaction, causing Maillard reaction, generating substances with special flavor, improving the flavor of protein hydrolysate, and thus enhancing the feeding desire of piglets.

[0024] 3. The present invention uses yeast cell wall polysaccharide as raw material, and after sulfonation modification, its immune activity is further enhanced, and the nanoliposome-yeast cell wall polysaccharide complex prepared can form a protective film on the surface of the intestine, reduce the damage of harmful substances to the intestinal mucosa, promote the repair and regeneration of the intestinal mucosa, maintain the normal structure and function of the intestine, and can be better absorbed and utilized by the piglet body, thereby improving the piglet's immune function and enhancing resistance to pathogens. In addition, the addition of astragalus polysaccharide and β-glucan jointly regulates the piglet's immune system, enhances the body's resistance, reduces the occurrence of diseases, and improves the survival rate and growth performance of piglets. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] Embodiment 1:

[0027] This embodiment provides a method for preparing a modified protein hydrolysate, comprising the following steps:

[0028] S1. Grind soybean meal, meat and bone meal and cottonseed meal respectively, pass through a 60-mesh sieve, mix them evenly in the ratio of 5:3:3 to obtain a protein substance, take 10 kg of the protein substance and add 80 L of deionized water to prepare a slurry to obtain a substrate solution; adjust the pH value to 7 with 1 mol / L hydrochloric acid or sodium hydroxide solution, preheat at 50°C, add 150 g of a composite enzyme, wherein the composite enzyme is a mixture of neutral protease, alkaline protease, cellulase and lipase in the ratio of 3:2:1:1, stir evenly, and perform enzymolysis for 4 hours, stirring every 30 minutes during the period to obtain an enzymolysis solution;

[0029] S2. Add 3kg of glucose to 100kg of enzymatic hydrolysate, heat to react, the heating temperature is 90°C, the reaction time is 1h, to obtain a composite solution, ultra-high pressure treatment, the ultra-high pressure treatment pressure is 300MPa, the treatment time is 10min, and after cooling, 4kg of probiotics are added for fermentation. The probiotics are prepared from lactic acid bacteria and Bacillus in a ratio of 2:1. The fermentation temperature is 36°C, the fermentation time is 30h, and the fermentation is carried out under anaerobic conditions to obtain a fermentation broth, which is filtered and dried in low-temperature vacuum to obtain a modified enzymatic hydrolysate.

[0030] This embodiment further provides a method for preparing modified yeast cell wall polysaccharide, comprising the following steps:

[0031] Q1. Ultrafinely grind yeast cell wall polysaccharide to a particle size of 5 microns to obtain yeast cell wall polysaccharide powder. Weigh 1 kg of yeast cell wall polysaccharide powder and disperse it with 10 L of anhydrous ethanol. Add 50 g of pyridine and stir at a stirring speed of 200 r / min. Heat at a heating temperature of 40°C. Add 40 g of chlorosulfonic acid and stir while adding for 30 min. Continue to react for 3 h to obtain a reaction solution. Cool to room temperature. Pour the reaction solution into ice water to quench the reaction. The volume ratio of the reaction solution to ice water is 1:3. Centrifuge at a centrifugal speed of 4000 r / min for 10 min. Take the precipitate and wash it with deionized water until the pH value of the washing solution is 6.5. Dry it at a drying temperature of 50°C to obtain sulfonated yeast cell wall polysaccharide.

[0032] Q2. Weigh 100 mg of soybean lecithin and 20 mg of cholesterol, dissolve them in 10 mL of chloroform-methanol mixed solution, the chloroform-methanol mixed solution is obtained by mixing chloroform and methanol in a volume ratio of 2:1 to obtain a lipid solution. Weigh 20 mg of sulfonated yeast cell wall polysaccharide, dissolve it in 10 mL of phosphate buffer to obtain a sulfonated yeast cell wall polysaccharide solution, and add it dropwise to the lipid solution. The volume ratio of the lipid solution to the sulfonated yeast cell wall polysaccharide solution is 3:1. Stir while adding dropwise at a stirring speed of 150 r / min. Heat to 40°C, perform rotary evaporation, the vacuum degree of rotary evaporation is 0.08 MPa, the rotary evaporation time is 30 min, a lipid film is obtained, phosphate buffer is added for hydration, the hydration time is 1 h, ultrasonic treatment, the ultrasonic power is 200 W, the ultrasonic time is 10 min, the ultrasound is intermittent, the ultrasound is 10 seconds, and the interval is 5 seconds to obtain an emulsion, dialyze, the molecular weight cutoff of the dialysis bag is 8000 Da, the dialysis time is 24 hours, the dialysate is replaced every 4 hours during this period, and freeze-drying is performed to obtain modified yeast cell wall polysaccharide.

[0033] The present embodiment provides a piglet feeding attractant composition containing protein hydrolysate, comprising the following components by weight: 25kg corn starch, 15kg modified protein hydrolysate, 8kg lactose, 4kg modified yeast cell wall polysaccharide, 2kg fish oil, 0.4kg citric acid, 0.4kg fumaric acid, 0.4kg sodium chloride, 0.6kg garlic extract, 0.1kg mint extract, 0.2kg calcium carbonate, 0.1kg calcium hydrogen phosphate, 0.2kg sucrose, 0.05kg milk flavor, 0.03kg astragalus polysaccharide and 0.02kg β-glucan.

[0034] The method for preparing a piglet feeding attractant composition containing proteolytic enzyme hydrolysate comprises the following steps:

[0035] (1) corn starch, modified protein hydrolysate, modified yeast cell wall polysaccharide and calcium carbonate are crushed respectively and passed through an 80-mesh sieve; then milk flavoring agent, sucrose and part of corn starch are preliminarily stirred and mixed, the part of corn starch is 10% of the total mass of corn starch, the stirring speed is 30 r / min, the stirring time is 5 min, then, acidulant, sodium chloride, garlic extract, mint extract and immunopotentiator are added, and stirring is continued to obtain a preliminary mixture;

[0036] (2) Add the remaining corn starch, modified protein hydrolysate, lactose, and modified yeast cell wall polysaccharide to the preliminary mixture and stir at a speed of 50 r / min for 15 min to obtain a solid mixture. Spray fish oil on the surface of the solid mixture and stir. Finally, add calcium carbonate and calcium hydrogen phosphate to obtain a piglet feeding attractant composition containing protein hydrolysate.

[0037] Embodiment 2:

[0038] This embodiment provides a method for preparing a modified protein hydrolysate, comprising the following steps:

[0039] S1. Grind soybean meal, meat and bone meal and cottonseed meal respectively, pass through a 70-mesh sieve, mix them evenly in the ratio of 5:3:3 to obtain a protein, take 10 kg of the protein, add 100 L of deionized water to prepare a slurry, and obtain a substrate solution; adjust the pH value to 7.5 with 1 mol / L hydrochloric acid or sodium hydroxide solution, preheat at a temperature of 50-55°C, add 180 g of a composite enzyme, wherein the composite enzyme is a mixture of neutral protease, alkaline protease, cellulase and lipase in the ratio of 3.5:2:1:1, stir evenly, and perform enzymolysis for 5 hours, stirring every 30 minutes during the period, to obtain an enzymolysis solution;

[0040] S2. Add 4kg of glucose to 100kg of enzymatic hydrolysate, heat to react, the heating temperature is 95°C, the reaction time is 1.5h, to obtain a composite solution, ultra-high pressure treatment, the ultra-high pressure treatment pressure is 350MPa, the treatment time is 15min, and after cooling, 5kg of probiotics are added for fermentation. The probiotics are prepared from lactic acid bacteria and Bacillus in a ratio of 2:1.5. The fermentation temperature is 37°C, the fermentation time is 40h, and the fermentation is carried out under anaerobic conditions to obtain a fermentation liquid, which is filtered and dried in low-temperature vacuum to obtain a modified protein hydrolysate.

[0041] This embodiment further provides a method for preparing modified yeast cell wall polysaccharide, comprising the following steps:

[0042] Q1. Ultrafinely grind yeast cell wall polysaccharide to a particle size of 8 microns to obtain yeast cell wall polysaccharide powder. Weigh 1 kg of yeast cell wall polysaccharide powder and disperse it with 13 L of anhydrous ethanol. Add 80 g of pyridine dropwise, stir at a stirring speed of 250 r / min, heat at a heating temperature of 45°C, add 80 g of chlorosulfonic acid dropwise, stir while dropping, add dropwise for 50 min, continue to react for 3.5 h, obtain a reaction solution, cool to room temperature, pour the reaction solution into ice water to quench the reaction, the volume ratio of the reaction solution to ice water is 1:4, centrifuge at a centrifugal speed of 4500 r / min, and the centrifugal time is 12 min. Take the precipitate, wash with deionized water until the pH value of the washing solution is 7, dry at a drying temperature of 55°C, and obtain sulfonated yeast cell wall polysaccharide.

[0043] Q2. Weigh 150 mg of soybean lecithin and 30 mg of cholesterol, dissolve them in 15 mL of chloroform-methanol mixed solution, the chloroform-methanol mixed solution is obtained by mixing chloroform and methanol in a volume ratio of 2:1 to obtain a lipid solution. Weigh 40 mg of sulfonated yeast cell wall polysaccharide, dissolve it in 15 mL of phosphate buffer to obtain a sulfonated yeast cell wall polysaccharide solution, and add it dropwise to the lipid solution. The volume ratio of the lipid solution to the sulfonated yeast cell wall polysaccharide solution is 5:1. Stir while adding dropwise at a stirring speed of 180 r / min and heat to 45 ° C, rotary evaporation, rotary evaporation vacuum degree of 0.08 MPa, rotary evaporation time of 50 min, to obtain a lipid film, add phosphate buffer for hydration, hydration time is 1-2 h, ultrasonic treatment, ultrasonic power is 250 W, ultrasonic time is 13 min, ultrasound is intermittent, ultrasound is 10 seconds, interval 5 seconds, to obtain an emulsion, dialyze, the dialysis bag has a molecular weight cutoff of 10000 Da, the dialysis time is 32 hours, during which the dialysate is replaced every 5 hours, freeze-dried, to obtain a modified yeast cell wall polysaccharide.

[0044] The present embodiment provides a piglet feeding attractant composition containing protein hydrolysate, comprising the following components by weight: 20kg corn starch, 18kg modified protein hydrolysate, 10kg lactose, 5kg modified yeast cell wall polysaccharide, 2.5kg fish oil, 0.5kg citric acid, 0.5kg fumaric acid, 0.5kg sodium chloride, 0.7kg garlic extract, 0.2kg mint extract, 0.4kg calcium carbonate, 0.2kg calcium hydrogen phosphate, 0.3kg sucrose, 0.1kg milk flavoring agent, 0.08kg astragalus polysaccharide and 0.06kg β-glucan.

[0045] The method for preparing a piglet feeding attractant composition containing proteolytic enzyme hydrolysate comprises the following steps:

[0046] (1) corn starch, modified protein hydrolysate, modified yeast cell wall polysaccharide and calcium carbonate are crushed respectively and passed through a 90-mesh sieve; then milk flavoring agent, sucrose and part of corn starch are preliminarily stirred and mixed, the part of corn starch is 15% of the total mass of corn starch, the stirring speed is 40 r / min, the stirring time is 8 min, then acidulant, sodium chloride, garlic extract, mint extract and immunopotentiator are added, and stirring is continued to obtain a preliminary mixture;

[0047] (2) Add the remaining corn starch, modified protein hydrolysate, lactose, and modified yeast cell wall polysaccharide to the preliminary mixture, stir at a speed of 55 r / min for 18 min to obtain a solid mixture, spray fish oil on the surface of the solid mixture, stir, and finally add calcium carbonate and calcium hydrogen phosphate to obtain a piglet feeding attractant composition containing protein hydrolysate.

[0048] Embodiment 3:

[0049] This embodiment provides a method for preparing a modified protein hydrolysate, comprising the following steps:

[0050] S1. Grind soybean meal, meat and bone meal and cottonseed meal respectively, pass through an 80-mesh sieve, mix them evenly in the ratio of 5:3:3 to obtain a protein substance, take 10 kg of the protein substance and add 120 L of deionized water to prepare a slurry to obtain a substrate solution; adjust the pH value to 8 with 1 mol / L hydrochloric acid or sodium hydroxide solution, preheat at 55°C, add 200 g of a composite enzyme, wherein the composite enzyme is a mixture of neutral protease, alkaline protease, cellulase and lipase in the ratio of 4:2:1:1, stir evenly, and perform enzymolysis for 6 hours, stirring every 30 minutes during the period, to obtain an enzymolysis solution;

[0051] S2. Add 5 kg of glucose to 100 kg of enzymatic hydrolysate, heat to react, the heating temperature is 100°C, the reaction time is 2 hours, to obtain a composite solution, ultra-high pressure treatment, the ultra-high pressure treatment pressure is 400 MPa, the treatment time is 20 minutes, after cooling, add 6 kg of probiotics for fermentation, the probiotics are prepared from lactic acid bacteria and Bacillus in a ratio of 2:2, the fermentation temperature is 38°C, the fermentation time is 48 hours, the fermentation is carried out under anaerobic conditions, and the fermentation liquid is obtained, filtered, and low-temperature vacuum dried to obtain a modified protein hydrolysate.

[0052] This embodiment further provides a method for preparing modified yeast cell wall polysaccharide, comprising the following steps:

[0053] Q1. Ultrafinely grind yeast cell wall polysaccharide to a particle size of 10 microns to obtain yeast cell wall polysaccharide powder. Weigh 1 kg of yeast cell wall polysaccharide powder and disperse it with 15 L of anhydrous ethanol. Add 100 g of pyridine dropwise, stir at a stirring speed of 300 r / min, heat at a heating temperature of 50°C, add 100 g of chlorosulfonic acid dropwise, stir while adding dropwise, add dropwise for 60 min, continue to react for 4 h, obtain a reaction solution, cool to room temperature, pour the reaction solution into ice water to quench the reaction, the volume ratio of the reaction solution to ice water is 1:5, centrifuge at a centrifugal speed of 5000 r / min, and the centrifugal time is 15 min. Take the precipitate, wash with deionized water until the pH value of the washing solution is 7.5, dry at a drying temperature of 60°C, and obtain sulfonated yeast cell wall polysaccharide.

[0054] Q2. Weigh 200 mg of soybean lecithin and 50 mg of cholesterol, dissolve them in 20 mL of chloroform-methanol mixed solution, the chloroform-methanol mixed solution is obtained by mixing chloroform and methanol in a volume ratio of 2:1 to obtain a lipid solution. Weigh 50 mg of sulfonated yeast cell wall polysaccharide, dissolve it in 20 mL of phosphate buffer to obtain a sulfonated yeast cell wall polysaccharide solution, and add it dropwise to the lipid solution. The volume ratio of the lipid solution to the sulfonated yeast cell wall polysaccharide solution is 8:1. Stir while adding dropwise at a stirring speed of 250 r / min. Heat to 5 0℃, rotary evaporation, the rotary evaporation vacuum degree is 0.09MPa, the rotary evaporation time is 60min, a lipid film is obtained, phosphate buffer is added for hydration, the hydration time is 2h, ultrasonic treatment, the ultrasonic power is 300W, the ultrasonic time is 10-15min, the ultrasound is intermittent, the ultrasound is 10 seconds, and the interval is 5 seconds to obtain an emulsion, dialysis, the dialysis bag has a molecular weight cutoff of 14000Da, the dialysis time is 48 hours, the dialysate is replaced every 4-6 hours during this period, and freeze-drying is performed to obtain modified yeast cell wall polysaccharide.

[0055] The present embodiment provides a piglet appetite attracting composition containing protein hydrolysate, comprising the following components by weight: 15kg corn starch, 22kg modified protein hydrolysate, 12kg lactose, 6kg modified yeast cell wall polysaccharide, 3kg fish oil, 0.6kg citric acid, 0.6kg fumaric acid, 0.6kg sodium chloride, 0.8kg garlic extract, 0.3kg mint extract, 0.5kg calcium carbonate, 0.3kg calcium hydrogen phosphate, 0.4kg sucrose, 0.2kg milk flavoring agent, 0.12kg astragalus polysaccharide and 0.08kg β-glucan.

[0056] The method for preparing a piglet feeding attractant composition containing proteolytic enzyme hydrolysate comprises the following steps:

[0057] (1) corn starch, modified protein hydrolysate, modified yeast cell wall polysaccharide and calcium carbonate are crushed respectively and passed through a 100-mesh sieve; then milk flavoring agent, sucrose and part of corn starch are preliminarily stirred and mixed, the part of corn starch is 20% of the total mass of corn starch, the stirring speed is 50 r / min, the stirring time is 10 min, then, acidulant, sodium chloride, garlic extract, mint extract and immunopotentiator are added, and stirring is continued to obtain a preliminary mixture;

[0058] (2) Add the remaining corn starch, modified protein hydrolysate, lactose, and modified yeast cell wall polysaccharide to the preliminary mixture and stir at a speed of 60 r / min for 20 min to obtain a solid mixture. Spray fish oil on the surface of the solid mixture and stir. Finally, add calcium carbonate and calcium hydrogen phosphate to obtain a piglet feeding attractant composition containing protein hydrolysate.

[0059] Comparative Example 1

[0060] Comparative Example 1 Compared with Example 3, in the preparation process of the piglet feeding attractant composition in Comparative Example 1, the modified protein hydrolysate was replaced with meat and bone meal, and other conditions remained unchanged.

[0061] Comparative Example 2

[0062] Comparative Example 2 Compared with Example 3, in the preparation process of the piglet feeding attractant composition in Comparative Example 2, the modified yeast cell wall polysaccharide was replaced with yeast cell wall polysaccharide, and other conditions remained unchanged.

[0063] Experimental example:

[0064] 25 weaned piglets aged 22 days were selected and randomly divided into 5 groups. The test period was 14 days. The 5 groups of piglets were fed with the piglet attractant composition prepared according to the method of Examples 1-3 and Comparative Examples 1-2, respectively. All piglets were fed 4 times a day during the whole period (6:00 am, 11:00 am, 4:00 pm, and 9:00 pm). The pig house was thoroughly cleaned and disinfected before the start of the test. After the start of the test, the pig house was cleaned every day and disinfected once a week. During the test, the piglets were raised at a temperature of 21-24°C; the relative humidity of the pig house was maintained at 65-75%; ventilation was maintained, and the mental state of the test piglets was observed.

[0065] The growth performance of piglets was observed and measured. The feed intake, weight change and diarrhea of ​​each piglet were measured and counted every day, and the average feed intake, average daily weight gain and diarrhea incidence of each piglet were calculated. The diarrhea incidence (%) = (number of piglets with diarrhea within 14 days / total number of piglets within 14 days) × 100%. The results are shown in Table 1.

[0066] Table 1

[0067] Group Average daily feed intake of piglets / g Average daily weight gain of piglets / g Diarrhea incidence / % Example 1 283 204 0 Example 2 305 239 0 Example 3 320 250 0 Comparative Example 1 195 140 40％ Comparative Example 2 198 145 40％

[0068] According to the results in Table 1, the piglet feeding attractant composition prepared in Examples 1-3 of the present invention significantly increases the average daily feed intake of piglets and reduces the incidence of diarrhea. By comparing Comparative Examples 1-2 and Examples 1-3, it can be seen that adding modified proteolytic enzymes and modified yeast cell wall polysaccharides can improve the growth performance of piglets.

[0069] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

[0070] The preferred embodiments of the present invention disclosed above are only used to help explain the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to only specific implementation methods. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A piglet feeding attractant composition containing proteolytic enzymes, characterized in that: The invention comprises the following components in parts by weight: 15-25 parts of corn starch, 15-22 parts of modified protein hydrolysate, 8-12 parts of lactose, 4-6 parts of modified yeast cell wall polysaccharide, 2-3 parts of fish oil, 0.8-1.2 parts of acidulant, 0.4-0.6 parts of sodium chloride, 0.6-0.8 parts of garlic extract, 0.1-0.3 parts of mint extract, 0.2-0.5 parts of calcium carbonate, 0.1-0.3 parts of calcium hydrogen phosphate, 0.2-0.4 parts of sucrose, 0.05-0.2 parts of milk flavoring agent and 0.05-0.2 parts of immunopotentiator; The modified protein hydrolysate is obtained by enzymatic hydrolysis and modification of soybean meal, meat and bone meal and cottonseed meal; The modified yeast cell wall polysaccharide is obtained by compounding the yeast cell wall polysaccharide with soybean lecithin and cholesterol after the polysaccharide is modified by sulfonation.

2. The piglet feeding attractant composition containing protein hydrolysate according to claim 1, characterized in that: The acidulant is an equal mixture of citric acid and fumaric acid; the immunopotentiator is a mixture of astragalus polysaccharide and beta-glucan in a mass ratio of 3:1-2.

3. The piglet feeding attractant composition containing protein hydrolysate according to claim 1, characterized in that: The method for preparing a modified protein hydrolysate comprises the following steps: S1. Grind soybean meal, meat and bone meal and cottonseed meal respectively, sieve, mix evenly according to proportion, add deionized water to adjust the slurry to obtain a substrate solution; adjust the pH of the substrate solution, preheat, add complex enzyme, stir evenly, and perform enzymolysis to obtain an enzymolysis solution; S2, adding glucose to the enzymatic hydrolysate, heating for reaction, obtaining a composite solution, treating with ultra-high pressure, adding probiotics for fermentation after cooling, obtaining a fermentation liquid, filtering, and low-temperature vacuum drying to obtain a modified enzymatic hydrolysate.

4. The piglet feeding attractant composition containing protein hydrolysate according to claim 3, characterized in that: In S1, soybean meal, meat and bone meal and cottonseed meal are mixed in a mass ratio of 5:3:3, the solid-liquid ratio of pulping is 1:8-12, the pH value is adjusted to 7-8 with 1 mol / L hydrochloric acid or sodium hydroxide solution, the composite enzyme is a neutral protease, an alkaline protease, a cellulase and a lipase mixed in a ratio of 3-4:2:1:1, and the mass ratio of the composite enzyme to the substrate is 1.5-2:

100.

5. The piglet feeding attractant composition containing protein hydrolysate according to claim 3, characterized in that: The mass ratio of glucose to enzymolyzate in S2 is 3-5:100, the mass ratio of probiotics to enzymolyzate is 4-6:100, and the probiotics are prepared from lactic acid bacteria and bacillus according to 2:1-2.

6. The piglet feeding attractant composition containing protein hydrolysate according to claim 1, characterized in that: The method for preparing modified yeast cell wall polysaccharide comprises the following steps: Q1. Ultrafinely grind yeast cell wall polysaccharide to obtain yeast cell wall polysaccharide powder, weigh the yeast cell wall polysaccharide powder and disperse it with anhydrous ethanol, drop pyridine, stir, heat, drop chlorosulfonic acid, stir while dropping, continue to react after the drop addition is completed, and after the reaction is completed, obtain a reaction solution, cool it to room temperature, pour the reaction solution into ice water to quench the reaction, centrifuge, take the precipitate, wash, and dry to obtain sulfonated yeast cell wall polysaccharide; Q2. Weigh soybean lecithin and cholesterol, dissolve them in a chloroform-methanol mixed solution to obtain a lipid solution, weigh sulfonated yeast cell wall polysaccharide, dissolve them in a phosphate buffer to obtain a sulfonated yeast cell wall polysaccharide solution, add the solution dropwise to the lipid solution, stir while adding, heat, and rotary evaporate to obtain a lipid film, add a phosphate buffer to hydrate, perform ultrasonic treatment to obtain an emulsion, dialyze, and freeze-dry to obtain a modified yeast cell wall polysaccharide.

7. The piglet feeding attractant composition containing protein hydrolysate according to claim 6, characterized in that: The mass volume ratio of yeast cell wall polysaccharide powder to anhydrous ethanol in Q1 is 1:10-15 g / mL, the mass ratio of yeast cell wall polysaccharide powder to pyridine is 1:0.05-0.1, the mass ratio of chlorosulfonic acid to yeast cell wall polysaccharide is 4-10:100, and the volume ratio of reaction solution to ice water is 1:3-5.

8. The piglet feeding attractant composition containing protein hydrolysate according to claim 6, characterized in that: The dosage ratio of soybean lecithin, cholesterol and chloroform-methanol mixed solution in Q2 is 100-200 mg: 20-50 mg: 10-20 mL, the chloroform-methanol mixed solution is obtained by mixing chloroform and methanol in a volume ratio of 2: 1, the dosage ratio of sulfonated yeast cell wall polysaccharide and phosphate buffer is 20-50 mg: 10-20 mL, and the volume ratio of lipid solution to sulfonated yeast cell wall polysaccharide solution is 3-8:

1.

9. A method for preparing a piglet feeding attractant composition containing protein hydrolysate, characterized in that: The following steps are involved: (1) corn starch, modified protein hydrolysate, modified yeast cell wall polysaccharide and calcium carbonate are crushed and sieved respectively; then milk flavoring agent, sucrose and part of corn starch are preliminarily stirred and mixed, then acidulant, sodium chloride, garlic extract, mint extract and immunopotentiator are added, and stirring is continued to obtain a preliminary mixture; (2) Add the remaining corn starch, modified protein hydrolysate, lactose, and modified yeast cell wall polysaccharide to the preliminary mixture, stir to obtain a solid mixture, spray fish oil on the surface of the solid mixture, stir, and finally add calcium carbonate and calcium hydrogen phosphate to obtain a piglet feeding attractant composition containing protein hydrolysate.

10. The method for preparing the piglet feeding attractant composition containing protein hydrolysate according to claim 9, characterized in that: In step (1), the portion of corn starch is 10-20% of the total mass of corn starch.