An emotional beauty composition for enhancing cell vitality and reducing wrinkles, and its preparation method and application

Abstract

The present invention discloses a mood beauty composition for enhancing cell vitality and reducing wrinkles, as well as its preparation method and application, belonging to the technical field of cosmetics; the mood beauty composition provided by the present invention includes the following components in parts by mass: 0.1-5 parts of Andrographis paniculata extract, 0.1-5 parts of Bacillus fermentation product, 0.1-5 parts of 4-tert-butyl cyclohexanol, and 0.1-5 parts of Pseudostellariae Radix extract. The present invention selects components in appropriate parts by mass to have excellent compounding effects, and the obtained mood beauty composition can effectively provide intrinsic protection for the skin by improving emotional state, reducing skin stress, and enhancing cell vitality; at the same time, it can also promote the self-repair ability of the skin, enhance the skin barrier repair effect, enhance the antioxidant and anti-inflammatory effects and provide external protection for the skin; that is, it interacts from two channels, internal and external, to achieve effective anti-aging effects and skin barrier repair effects.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to an emotional beauty composition for enhancing cell vitality and reducing wrinkles, as well as a preparation method and application thereof. Background Art

[0002] Emotional beauty refers to a new beauty concept that improves skin health and appearance through mood regulation and stress relief. In recent years, emotional beauty has gradually attracted attention in the fields of dermatology and anti-aging. Studies have found that emotional state has a significant impact on skin health, especially negative emotions (such as anxiety, depression, and stress), which can accelerate the aging process and affect the skin's appearance. The core concept of emotional beauty is to promote the synergistic effects of internal and external skin health by regulating mood fluctuations and reducing stress, thereby achieving the effect of delaying aging and improving skin quality.

[0003] There's a close relationship between emotions and aging. Negative emotions trigger stress responses, particularly excessive secretion of stress hormones like cortisol, which accelerate the aging process. Chronic stress and anxiety not only affect the skin's barrier function, leading to dryness and dehydration, but also damage collagen and elastic fibers in the skin through oxidative stress and inflammation, causing sagging and the appearance of fine lines and wrinkles. The impact of emotions on aging is gradual; chronic emotional instability accelerates the appearance of aging, while positive, balanced emotions help maintain youthful skin.

[0004] Currently, many anti-aging technologies, such as antioxidants, fillers, lasers, and microneedles, primarily rely on external interventions to improve the skin's appearance or stimulate skin repair. However, most of these approaches focus on surface or localized effects on the skin, ignoring the profound impact of emotions on aging. Long-term reliance on these approaches can also lead to side effects. Therefore, developing a composition that can achieve anti-aging effects by improving mood has become a research hotspot. Summary of the Invention

[0005] The purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide an emotional beauty composition, as well as a preparation method and application thereof, which can achieve excellent anti-aging effects by improving emotional state, reducing skin stress and enhancing cell vitality, and can achieve anti-aging effects by enhancing skin self-repair ability, improving skin barrier repair effect, and enhancing antioxidant and anti-inflammatory effects, and is mild and non-irritating, suitable for sensitive skin, and can enhance cell vitality and reduce wrinkles.

[0006] To achieve the above objectives, in a first aspect of the present invention, the present invention provides a mood beauty composition for enhancing cell vitality and reducing wrinkles, wherein the mood beauty composition comprises the following components in parts by weight:

[0007] 0.1-5 parts of Andrographis paniculata extract, 0.1-5 parts of Bacillus fermentation product, 0.1-5 parts of 4-tert-butyl cyclohexanol, and 0.1-5 parts of Pseudostellariae pseudoginseng extract.

[0008] The present invention selects components in appropriate mass proportions, and the components have good synergistic effects. It can not only effectively provide internal protection for the skin by improving emotional state, reducing skin stress, and enhancing cell vitality, but also provide external protection for the skin by improving the skin's self-repair ability, enhancing antioxidant and anti-inflammatory effects, and improving the skin barrier repair ability. That is, the emotional beauty composition provided by the present invention can achieve effective anti-aging effects from the inside out; and the emotional beauty composition provided by the present invention is mild and non-irritating, and is suitable for sensitive skin.

[0009] Specifically, the emotional beauty composition provided by the present invention can achieve anti-aging effects through the dual mechanisms of regulating the skin and psychology. On the one hand, the emotional beauty composition provided by the present invention can regulate neurotransmitter and hormone levels through the synergistic effect between the components, reduce stress reactions, and reduce the secretion of negative hormones such as cortisol, thereby avoiding the stimulation of excessive aging. For example, the emotional beauty composition provided by the present invention can affect the expression of bitter taste receptor genes in the body, affect the activity of β-galactosidase, and affect the secretion of cortisol, etc. On the other hand, the emotional beauty composition provided by the present invention can promote the skin's self-repair ability, enhance immunity, and antioxidant and anti-inflammatory ability through the synergistic effect between the components, thereby further delaying the aging process and achieving excellent anti-aging effects. Through the effects of the above two aspects, the present invention can achieve a more effective, comprehensive and long-lasting anti-aging effect.

[0010] Specifically, Andrographis paniculata extract is rich in andrographolide, which can activate bitter receptors on the skin, regulate the skin's antioxidant and anti-inflammatory mechanisms, and slow down the aging process; Bacillus fermentation product is a natural ingredient produced by Bacillus fermentation, which can reduce the level of the stress hormone cortisol, reduce the negative impact of emotional fluctuations on the skin, protect the skin from damage caused by emotional stress, and enhance the skin barrier function; 4-tert-butyl cyclohexanol is an inhibitor of the pain receptor TRPV1, which can help the anti-aging process by reducing inflammatory responses, relieving skin allergies, and regulating the neuro-endocrine-immune axis; Pseudostellaria baicalensis extract is rich in polysaccharides and cyclic peptides, which can improve the structure of the epidermis and dermis by enhancing the skin's resistance to pressure. Pseudostellaria baicalensis has the same medicinal effects as ginseng but is milder, so it is more suitable for sensitive skin.

[0011] As a preferred embodiment of the mood beauty composition of the present invention, the mood beauty composition includes the following components in parts by mass: 0.5-3 parts of Andrographis paniculata extract, 0.5-3 parts of Bacillus fermentation product, 0.5-2 parts of 4-tert-butyl cyclohexanol, and 0.3-2.5 parts of Pseudostellariae pseudoginseng extract.

[0012] As a preferred embodiment of the mood beauty composition of the present invention, 1-1.8 parts of Andrographis paniculata extract, 1-2 parts of Bacillus fermentation product, 1-1.5 parts of 4-tert-butyl cyclohexanol, and 0.5-1 part of Pseudostellariae pseudoginseng extract are included.

[0013] The present invention has found that the mass parts of the components in the mood beauty composition will affect the comprehensive performance of the composition. When the mass parts of the components in the mood beauty composition are further selected to be within the above-mentioned range, the obtained mood beauty composition can better increase the expression of bitter receptor genes, reduce the activity of β-galactosidase, and improve the secretion of cortisol, thereby achieving better mood improvement and anti-aging effects, which is reflected in better wrinkle improvement and stronger skin barrier repair ability.

[0014] As a preferred embodiment of the mood beauty composition of the present invention, the mass ratio of the Andrographis paniculata extract to 4-tert-butyl cyclohexanol is 1:(0.8-4).

[0015] Exemplarily, the mass ratio of the Andrographis paniculata extract and 4-tert-butyl cyclohexanol can be any point value or any two-point range value between 1:(0.8-4), such as 1:0.8, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, etc.

[0016] The present invention has found that in the emotional beauty composition, the mass ratio of Andrographis paniculata extract and 4-tert-butyl cyclohexanol not only affects the compatibility between the two, thereby affecting the synergistic effect between them, but also affects the interaction between the two and other components; when the mass ratio of Andrographis paniculata extract and 4-tert-butyl cyclohexanol is further selected to be 1:(0.8-4), the obtained emotional beauty composition has better skin barrier repair ability and better ability to improve mood, thereby achieving better anti-aging effect.

[0017] As a preferred embodiment of the mood beauty composition of the present invention, the mass percentage of the Bacillus fermentation product is ≥15% based on the total mass of the mood beauty composition.

[0018] Exemplarily, based on the total mass of the mood beauty composition, the mass percentage of the Bacillus fermentation product can be any point value or any two-point range value ≥15%, for example, it can be 15-30%, 15-40%, 15-50%, 15-60%, etc., or it can be 15%, 17%, 19%, 20%, 22%, 24%, 26%, 28%, 30%, 32%, 35%, 40%, 45%, 50%, 55%, 60%, etc.

[0019] Preferably, based on the total mass of the mood beauty composition, the mass percentage of the Bacillus fermentation product is 30-38%.

[0020] The present invention has found that the mass percentage of Bacillus fermentation products in the mood beauty composition affects the product's anti-aging ability and skin barrier repair ability. When the mass percentage of Bacillus fermentation products is further selected to be ≥15%, especially 30-38%, the comprehensive effect of the obtained mood beauty composition is better.

[0021] As a preferred embodiment of the mood beauty composition of the present invention, the preparation method of the Bacillus fermentation product comprises the following steps:

[0022] The Bacillus is inoculated into a fermentation medium and fermented under oxygen conditions at a temperature of 30-40°C for 24-72 hours. After the fermentation is completed, the culture medium is crushed under a pressure of 1000-1500 bar, and then centrifuged to obtain the supernatant to obtain the Bacillus fermentation product.

[0023] The fermentation medium formula is: 18-22g carbon source, 8-12g nitrogen source, 0.4-0.6g sodium sulfate, 1.8-2.2g sodium dihydrogen phosphate, 0.08-0.12g calcium chloride, 0.18-0.22g magnesium sulfate, 0.48-0.52g sodium chloride, 0.48-0.52g sodium glutamate, and distilled water is added to 1000mL.

[0024] Preferably, the fermentation temperature is 37±2°C; and the fermentation time is 48±2h.

[0025] Preferably, after fermentation, the milk is crushed at a pressure of 1200±100 bar.

[0026] Preferably, the crushing is performed 2-3 times.

[0027] Preferably, the dissolved oxygen content in the fermentation medium is 10%-30%.

[0028] It should be noted that the dissolved oxygen content in the fermentation medium was achieved by controlling the amount of oxygen introduced; the dissolved oxygen content in the fermentation medium was detected using a dissolved oxygen meter (Shanghai Instrument and Electronics Science Co., Ltd., JPBJ-608).

[0029] As a preferred embodiment of the mood beauty composition of the present invention, the Bacillus includes at least one of Bacillus marinus, Bacillus subtilis, Bacillus licheniformis, and Bacillus megaterium.

[0030] Preferably, the Bacillus comprises Bacillus marinus.

[0031] For example, the Bacillus marinus may be Oceanobacillus profundus with the product number bio-02923 of Beijing Biobowei Biotechnology Co., Ltd.; the Bacillus subtilis may be Bacillus subtilis with the product number 2018009 of Jinan Jinyuyuan Biotechnology Co., Ltd.

[0032] As a preferred embodiment of the mood beauty composition of the present invention, the carbon source includes at least one of oat extract, glucose, sucrose, and soybean extract.

[0033] For example, the oat extract may be the oat extract with the product number HK330 produced by Shaanxi Huike Plant Development Co., Ltd.; the soybean extract may be the soybean powder with the product number Q / SJKL0001S produced by Jiangsu Junkanglai Health Industry Co., Ltd.

[0034] As a preferred embodiment of the mood beauty composition of the present invention, the nitrogen source includes peptone.

[0035] As a preferred embodiment of the mood beauty composition of the present invention, the inoculation amount of the Bacillus is 10^9 to 10^10 CFU / mL.

[0036] As a preferred embodiment of the mood beauty composition of the present invention, the centrifugal speed is 8000-12000 rpm, and the centrifugal time is 5-15 minutes.

[0037] The present invention has found that the preparation method and preparation parameters of the Bacillus fermentation product will affect the comprehensive effect of the mood beauty composition to a certain extent. When the preparation method and preparation parameters of the Bacillus fermentation product are further selected within the above range, the comprehensive effect of the obtained product is better.

[0038] It should be noted that the Andrographis paniculata extract in the mood beauty composition of the present invention can be purchased commercially or prepared using a conventional extraction method. For example, the Andrographis paniculata extract can be obtained by heating and refluxing an ethanol solution or by ultrasonic extraction.

[0039] It should be noted that the Pseudostellariae Radix extract in the mood beauty composition of the present invention can be purchased commercially or prepared using a conventional extraction method. For example, the Pseudostellariae Radix extract can be obtained by heating and refluxing an ethanol solution or by ultrasonic extraction.

[0040] In a second aspect of the present invention, the present invention provides use of the mood beauty composition in preparing skin care products.

[0041] The emotional beauty composition provided by the present invention can not only fight aging through external physical intervention through systemic regulation, but also can fundamentally reduce the causes of aging from the inside by balancing internal emotions and regulating psychological and physiological states; thereby achieving a more comprehensive, effective and long-lasting anti-aging effect; at the same time, the emotional beauty composition provided by the present invention also has excellent skin barrier repair ability; and is mild and non-irritating, suitable for sensitive skin; therefore, it can be widely used as an effective ingredient in the preparation of skin care products.

[0042] As a preferred embodiment of the application of the present invention, the skin care product includes any one of lotion, emulsion, ointment, cream, facial mask, essence, and spray.

[0043] In the third aspect of the present invention, the present invention provides an emulsion comprising the following components in mass percentage: 1-10% of the emotional beauty composition of the present invention, 0.05-0.3% thickener, 0.5-1% moisturizer, 0.01-0.3% pH regulator, 0.5-5% emulsifier, 0.5-3% preservative, and the balance deionized water.

[0044] The present invention has found that by adding the emotional beauty composition of the present invention to the emulsion provided by the present invention, it can better achieve the effects of internal and external channels, thereby achieving excellent anti-aging effects and skin barrier repair effects.

[0045] As a preferred embodiment of the emulsion of the present invention, the thickener includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol.

[0046] As a preferred embodiment of the emulsion of the present invention, the moisturizing agent includes at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butylene glycol, glycerol, budding septin polysaccharide, and ceramide.

[0047] As a preferred embodiment of the emulsion of the present invention, the pH regulator includes at least one of arginine, tromethamine, and EDTA-disodium.

[0048] As a preferred embodiment of the emulsion of the present invention, the emulsifier includes at least one of caprylic capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetraisostearate, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate.

[0049] As a preferred embodiment of the emulsion of the present invention, the preservative includes at least one of 1,2-propylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, and acrylates / C10-30 alkyl acrylate crosspolymer.

[0050] Compared with the prior art, the present invention has the following beneficial effects:

[0051] The present invention selects components in appropriate mass ratios, specifically Andrographis paniculata extract, Bacillus fermentation product, 4-tert-butyl cyclohexanol, and Radix Pseudostellariae extract. These four ingredients have excellent compounding effects. The resulting emotional beauty composition can effectively provide intrinsic protection for the skin by improving emotional state, reducing skin stress, and enhancing cell vitality; at the same time, it can also promote the skin's self-repair ability, improve the skin barrier repair effect, and enhance the antioxidant and anti-inflammatory effects to provide extrinsic protection for the skin; that is, it interacts from both internal and external channels to achieve effective anti-aging effects and skin barrier repair effects. In addition, the emotional beauty composition provided by the present invention is mild and non-irritating, and can be widely used in the preparation of skin care products to achieve good anti-aging and skin barrier repair effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] Figure 1 This is a comparison of eye wrinkles before and after 28 days of use in the blank application example and application example 1 in effect example 3. DETAILED DESCRIPTION

[0053] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0054] Unless otherwise specified, the reagents, methods, and equipment used in the present invention are all conventional reagents, methods, and equipment in the art; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials.

[0055] Andrographis paniculata extract: HKY002, Shaanxi Huike Plant Development Co., Ltd.;

[0056] Pseudostellaria heterophylla extract: HK3560, Shaanxi Huike Plant Development Co., Ltd.;

[0057] 4-tert-Butylcyclohexanol: SymSitive 1609, Symrise GmbH, Germany;

[0058] Almond extract: HK-66, Shaanxi Huike Plant Development Co., Ltd.;

[0059] Hops extract: HK029, Shaanxi Huike Plant Development Co., Ltd.;

[0060] Tetrandrine: Tetrandrine, Shaanxi Shize Tiancheng Biotechnology Co., Ltd.;

[0061] Ginseng extract: HK-72, Shaanxi Huike Plant Development Co., Ltd.;

[0062] Oat extract: HK330, Shaanxi Huike Plant Development Co., Ltd.;

[0063] Soybean extract: Q / SJKL0001S, Jiangsu Junkanglai Health Industry Co., Ltd.

[0064] Bacillus fermentation product 1: homemade, the preparation method includes the following steps:

[0065] Bacillus marinus (Bacillus marinus, Beijing Biobo Biotechnology Co., Ltd., bio-02923) was inoculated into a fermentation medium at an inoculum size of 5*10^9 CFU / mL and fermented under oxygen conditions (aerobic fermentation, controlling the dissolved oxygen content in the fermentation medium to 20%) at a temperature of 37°C for 48 hours. After the fermentation, the culture medium was crushed twice at a pressure of 1200 bar, followed by centrifugation at 10000 rpm for 10 minutes at 25°C, and the supernatant was collected to obtain the Bacillus fermentation product;

[0066] The formula of the fermentation medium is: 20g oat extract, 10g peptone, 0.5g sodium sulfate, 2g sodium dihydrogen phosphate, 0.1g calcium chloride, 0.2g magnesium sulfate, 0.5g sodium chloride, 0.5g monosodium glutamate, and distilled water is added to 1000mL.

[0067] Bacillus fermentation product 2: homemade. The only difference in preparation method from Bacillus fermentation product 1 is that Bacillus subtilis (Jinan Jinyuyuan Biotechnology Co., Ltd., 2018009) was used instead of Bacillus marineus.

[0068] Bacillus fermentation product 3: homemade, the only difference in preparation method from Bacillus fermentation product 1 is that soy extract is used instead of oat extract;

[0069] Bacillus fermentation product 4: homemade. The only difference in the preparation method from Bacillus fermentation product 1 is that the fermentation is carried out under anaerobic conditions (anaerobic fermentation, the dissolved oxygen content in the fermentation medium is approximately 0).

[0070] Examples 1-11 and Comparative Examples 1-4

[0071] The embodiments and comparative examples of the present invention provide a mood beauty composition, the components (parts by weight) of the composition are shown in Table 1;

[0072] Table 1

[0073]

[0074] The preparation method of the mood beauty composition provided in Example 1 is: mixing the components uniformly to obtain the mood beauty composition.

[0075] The preparation methods of the mood beauty compositions provided in Examples 2-11 and Comparative Examples 1-4 are consistent with that in Example 1; if relevant components are not present, they may be omitted.

[0076] Comparative Example 5

[0077] The comparative example of the present invention provides a mood beauty composition. The only difference between the mood beauty composition and Example 1 is that the Andrographis paniculata extract is replaced by the almond extract.

[0078] Comparative Example 6

[0079] The comparative example of the present invention provides a mood beauty composition, wherein the only difference between the mood beauty composition and Example 1 is that the hops extract is used instead of the Bacillus fermentation product.

[0080] Comparative Example 7

[0081] The comparative example of the present invention provides a mood beauty composition, wherein the only difference between the mood beauty composition and Example 1 is that tetrandrine is used instead of 4-tert-butyl cyclohexanol.

[0082] Comparative Example 8

[0083] The comparative example of the present invention provides a mood beauty composition, wherein the only difference between the mood beauty composition and Example 1 is that the Pseudostellariae Radix extract is replaced by the ginseng extract.

[0084] Application Examples 1-13, Comparative Application Examples 1-8, and Blank Application Examples

[0085] The application examples, comparative application examples, and blank application examples of the present invention provide an emulsion, the components (mass percentage) of the emulsion are shown in Table 2; wherein, the emotional beauty compositions used in Application Examples 1-11 are respectively the emotional beauty compositions prepared in Examples 1-11, for example, the emotional beauty composition used in Application Example 1 is the emotional beauty composition in Example 1, the emotional beauty composition used in Application Example 2 is the emotional beauty composition in Example 2, and so on; the emotional beauty compositions used in Comparative Application Examples 1-8 are respectively the emotional beauty compositions prepared in Comparative Examples 1-8; the emotional beauty compositions used in Application Examples 12-13 are the emotional beauty compositions prepared in Example 1;

[0086] Table 2

[0087]

[0088]

[0089] The preparation method of the emulsion provided in Application Example 1 comprises the following steps:

[0090] (1) Mixing a moisturizer, a thickener, and a portion of a pH adjuster (EDTA-disodium) with water, stirring, heating to 85°C, and homogenizing at 1300 rpm for 4 minutes. After homogenization, keep the mixture warm for later use to obtain prefabricated component A;

[0091] (2) Mix the emulsifier, heat to 75°C, and homogenize at 1300 rpm for 4 minutes. After homogenization, keep warm and set aside to obtain prefabricated component B;

[0092] (3) Mix the preservatives and heat to 60°C to melt to obtain prefabricated component C;

[0093] (4) The preformed component A is heated to 80°C, and the preformed component B is added at a speed of 300 rpm, and stirred and mixed. Then, the temperature is cooled to 60°C, and the preformed component C is added at a speed of 300 rpm and stirred and mixed. Then, the temperature is cooled to below 45°C, and the mood beauty composition is added and stirring is continued for 8 minutes. Finally, the remaining pH adjuster (arginine) is added to adjust the pH to 5.5-7.0. The stirring can be stopped, and the material is discharged to obtain an emulsion.

[0094] The preparation methods of the emulsions provided in Application Examples 2-13, Comparative Application Examples 1-8 and Blank Application Examples are consistent with that in Application Example 1, and no relevant components are added if they are not present.

[0095] Effect Example 1

[0096] The present invention investigates the performance of the mood beauty compositions prepared in Examples 1-11 and Comparative Examples 1-8. Unless otherwise specified, the reagents, cells, culture media, etc. used in parallel tests remain the same, including the following aspects:

[0097] 1. Effect of the emotional beauty composition on skin cell vitality under elevated stress

[0098] The cell line used was human dermal fibroblasts (HDF) (Cat. No. PC-202h, Wuhan Saios Biotechnology Co., Ltd.). The test conditions were: incubator temperature 37±1°C, humidity 90±5%, carbon dioxide 5±1%. Cells were cultured and treated according to grouping, and then tested. The specific test was cell viability testing. The test method is as follows:

[0099] (1) The cell suspension was seeded into a 96-well cell culture plate at a density of 2000 cells per well. 100 μL of DMEM high-glucose culture medium (Gibco) containing 10% fetal bovine serum was added to each well and cultured for 24 h.

[0100] (2) The supernatant was discarded, 100 μL of DMEM medium (2105341, Gibco) was added to the blank control group, 100 μL of DMEM medium containing 500 μM cortisol (Shanghai Yuanye Biotechnology, S31360) was added to the model group, and 100 μL of medium containing 0.5 wt% of the corresponding mood beauty composition (the mood beauty composition prepared in Example 1-11 and Comparative Example 1-8) and 500 μM cortisol was added to the sample group, and cultured for 24 h;

[0101] (3) Cell viability assay: CCK8 cell proliferation kit (Cat. No. C6005M, Suzhou Youyi Landi Biotechnology Co., Ltd.) was used to detect the cell viability of each group. The specific operation was to add 10 μL of CCK8 solution to each well, continue to incubate at 37°C for 2 h, shake for 30 s using a microplate reader, and detect the absorbance OD value at a wavelength of 450 nm on an enzyme-linked immunosorbent assay (ELISA).

[0102] In this experiment, the improvement of cell activity is expressed by cell survival rate, which is calculated as follows:

[0103] Cell viability = OD value 模型组或样品组 / OD value 空白对照组 ×100%;

[0104] The obtained cell viability results are shown in Table 3.

[0105] 2. Effects of the Mood Beauty Composition on Bitter Taste Receptor Gene Expression and β-Galactosidase Activity

[0106] Bitter taste receptors are not only present in the taste system but are also widely distributed in the skin. They participate in a variety of physiological reactions, playing a particularly important role in anti-oxidation, anti-inflammatory, and anti-aging. Higher bitter taste receptor expression indicates a stronger anti-aging ability of the mood beauty composition. Hydrogen peroxide induces cellular senescence, a hallmark of which is increased β-galactosidase activity. Therefore, lower β-galactosidase activity after sample treatment indicates lower levels of cellular senescence and stronger anti-aging properties of the mood beauty composition.

[0107] The cell line used in the test was human keratinocyte HaCaT (Guangzhou Geneo Biotechnology Co., Ltd.). The test conditions were: incubator temperature 37±1°C, humidity 90±5%, and carbon dioxide 5±1%. Cells were cultured and treated according to grouping, and then tested. Specific tests included bitter taste receptor gene expression detection and β-galactosidase activity testing. The test methods are as follows:

[0108] (1) The cell suspension was inoculated into a 96-well cell culture plate at a density of 2000 cells per well. 100 μL of culture medium was added to each well and cultured for 24 h.

[0109] (2) Induction: Treat with PBS buffer containing 50 μM hydrogen peroxide for 30 minutes, discard the PBS containing hydrogen peroxide, and then wash with PBS three times;

[0110] (3) Sampling: 100 μL of culture medium was added to the blank control group, and 100 μL of culture medium containing 0.5 wt % of the corresponding mood beauty composition (the mood beauty composition prepared in Examples 1-11 and Comparative Examples 1-8) was added to the sample groups, and the culture was continued for 24 h;

[0111] (4) Detection of bitter taste receptor gene expression: Real-time fluorescence quantitative PCR was used to detect the expression of the bitter taste receptor TAS2R10 gene in HaCaT cells of each group. GAPDH was used as the internal reference gene. The TAS2R10 primer sequences used were 5'-ACGTGTAGTGGAAGGCATCT-3' and 5'-TGAAATAGCTAAGCCGGTGAG-3', and the GAPDH primer sequences used were 5'-ACTTTGTCAAGCTCATTTCC-3' and 5'-TGCAGCGAACTTTATTGATG-3'.

[0112] (5) β-galactosidase activity assay: The β-galactosidase activity assay kit (Cat. No. ab287846, abcam) was used to detect the senescence level of cells;

[0113] (6) Calculate the bitter taste receptor increase rate and β-galactosidase activity reduction rate, where bitter taste receptor increase rate = (bitter taste receptor expression level 样品组Bitter taste receptor expression 空白组 -1)*100%; β-galactosidase activity reduction rate = 1-(β-galactosidase activity 样品组 / β-galactosidase activity 空白组 )*100%;

[0114] The obtained data are shown in Table 3.

[0115] Table 3

[0116]

[0117] As can be seen from Table 3, the mood beauty composition provided by the present invention has excellent effects of enhancing cell vitality under stress, increasing bitter taste receptor expression, and reducing β-galactosidase activity. Specifically, the obtained mood beauty composition has a cell survival rate of over 66.6% under stress, an increase rate of bitter taste receptors of over 30.3%, and a reduction rate of β-galactosidase activity of over 37.5%.

[0118] As can be seen from Examples 1-8, the mass fraction of the components affects the overall effect of the composition. When the mass fraction of the components is further selected within a preferred range, the overall effect of the resulting mood beauty composition is better. Specifically, the resulting mood beauty composition has a cell survival rate of greater than 77.3% under stress, an increase rate of bitter taste receptors of greater than 47.0%, and a decrease rate of β-galactosidase activity of greater than 50.0%.

[0119] It can be seen from Example 1 and Comparative Examples 1-4 that the comprehensive effects of the present invention cannot be achieved when any one of the components in the mood beauty composition is missing; it can be seen from Example 1 and Comparative Examples 5-8 that the effects of the present invention cannot be achieved when other components with similar functions are used to replace the components in the present invention.

[0120] Effect Example 2

[0121] The effect examples of the present invention explore the irritation of the emulsions prepared by application examples 1-13 and comparative application examples 1-8.

[0122] The test process was as follows: 30 volunteers, 15 men and 15 women, aged 20-50 years, were recruited. Using a closed patch test method, an equal amount (0.020mL-0.025mL) of the test sample was placed in a specific patch tester. This was then applied to the volunteer's arm with hypoallergenic tape, gently pressed to evenly adhere to the skin, and left in place for 24 hours. A blank control group received distilled water, and a negative control group received the same lotion as the blank application example. After 24 hours, the patch tester was removed, and skin reactions were observed 0.5 hours, 24 hours, and 48 hours later, with the results recorded. The levels of adverse skin reactions are shown in Table 4 below.

[0123] Table 4

[0124]

[0125] After testing, the emulsions provided in Application Examples 1-13 of the present invention and Comparative Application Examples 1-8 all showed negative reactions after human patch tests, that is, they were completely non-irritating to human skin, indicating that the emotional beauty composition disclosed in the present invention has low irritation.

[0126] Effect Example 3

[0127] The present invention investigates the effects of application examples 1-13, comparative application examples 1-8, and a blank application example on mood improvement, wrinkle reduction, and skin barrier repair in volunteers with sensitive skin, including the following steps:

[0128] According to the "Technical Specifications for Safety of Cosmetics" (2015), 132 Asian adult testers aged 40-60 who considered themselves to have sensitive skin were selected and randomly divided into 22 groups, with 6 people in each group. The volunteers used samples (the samples were the emulsions prepared from application examples 1-13, comparative application examples 1-8 and blank application examples) on their entire faces once in the morning and evening every day, and visited the clinic to collect data on the 0th day and the 28th day of use. After the visit, the volunteers washed their faces with facial cleanser and sat quietly in an air-conditioned room with a temperature of 21±1°C and a humidity of 50±10% for 30 minutes. Saliva samples were collected from the volunteers, and the human cortisol ELISA kit (product number ml711149, Enzyme-Linked Biology) was used to detect the volunteers' salivary cortisol levels to assess their psychological stress levels; the transepidermal water loss value TEWL of the cheekbone was measured using the skin water loss test probe TewaMeter; facial wrinkles were photographed and analyzed using VISIA-CR;

[0129] The mood-improving ability is represented by the improvement in salivary cortisol levels, the skin barrier repair ability is represented by the improvement in TEWL values, and the wrinkle-reducing effect is represented by the improvement in the area of ​​wrinkles at the tail of the eyes. The formula is as follows:

[0130] Improvement rate = (T0 样品组 -T28 样品组 ) / T0 样品组 *100%]-[(T0 空白应用例 -T28 空白应用例 ) / T0 空白应用例 *100%];

[0131] The results are shown in Table 5;

[0132] Table 5

[0133]

[0134]

[0135] As can be seen from Table 5, the emulsion prepared by the technical solution of the present invention can effectively improve mood, enhance skin barrier capacity, and delay aging; specifically, the obtained emulsion has an improvement rate of more than 22.6% on salivary cortisol, an improvement rate of more than 17.9% on TEWL value, and an improvement rate of more than 12.0% on wrinkles at the corners of the eyes;

[0136] As can be seen from Application Examples 1-8, the mass fraction of the components in the mood beauty composition affects the overall effect of the emulsion. When the mass fraction of the components in the further selected mood beauty composition is within the preferred range, the overall effect of the obtained emulsion is better. Specifically, the obtained emulsion has an improvement rate of more than 32.9% on salivary cortisol, an improvement rate of more than 24.2% on TEWL value, and an improvement rate of more than 16.6% on wrinkles at the corners of the eyes.

[0137] As can be seen from Application Example 1 and Application Examples 12-13, the amount of the emotional beauty composition added also affects the overall performance of the emulsion. To a certain extent, as the amount of the emotional beauty composition added increases, the overall effect of the emulsion shows a trend of being more excellent. When the amount of the emotional beauty composition added is further increased, the overall effect of the emulsion does not change significantly.

[0138] In addition, the eye wrinkles of a random volunteer in the blank application example and application example 1 before and after 28 days of use in the test process of the present invention are as follows: Figure 1 As shown, from Figure 1 It can be clearly seen that the emulsion provided by the present invention can effectively reduce wrinkles at the end of the eyes;

[0139] It can be seen from Application Example 1 and Comparative Application Examples 1-4 that when any one of the components in the emotional beauty composition is missing, the resulting emulsion cannot achieve the comprehensive effects of the present invention; it can be seen from Application Example 1 and Comparative Application Examples 5-8 that when other components with similar functions are used to replace the corresponding components in the emotional beauty composition, the resulting emulsion cannot achieve the effects of the present invention.

[0140] Finally, it should be noted that the above embodiments are intended to illustrate the technical solutions of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A mood beauty composition for enhancing cell vitality and reducing wrinkles, characterized in that: The mood beauty composition includes the following components in parts by weight: 0.1-5 parts of Andrographis paniculata extract, 0.1-5 parts of Bacillus fermentation product, 0.1-5 parts of 4-tert-butyl cyclohexanol, and 0.1-5 parts of Pseudostellariae pseudoginseng extract.

2. The mood beauty composition according to claim 1, characterized in that The mood beauty composition includes the following components in parts by weight: 0.5-3 parts of Andrographis paniculata extract, 0.5-3 parts of Bacillus fermentation product, 0.5-2 parts of 4-tert-butyl cyclohexanol, and 0.3-2.5 parts of Pseudostellariae pseudoginseng extract.

3. The mood beauty composition according to claim 1, characterized in that The mood beauty composition includes the following components in parts by weight: 1-1.8 parts of Andrographis paniculata extract, 1-2 parts of Bacillus fermentation product, 1-1.5 parts of 4-tert-butyl cyclohexanol, and 0.5-1 part of Pseudostellariae pseudoginseng extract.

4. The mood beauty composition according to claim 1, characterized in that The mass ratio of the Andrographis paniculata extract to 4-tert-butyl cyclohexanol is 1:(0.8-4).

5. The mood beauty composition according to claim 1, characterized in that Based on the total mass of the mood beauty composition, the mass percentage of the Bacillus fermentation product is ≥15%.

6. The mood beauty composition according to claim 1, characterized in that The preparation method of the Bacillus fermentation product comprises the following steps: The Bacillus is inoculated into a fermentation medium and fermented under oxygen conditions at a temperature of 30-40°C for 24-72 hours. After the fermentation is completed, the culture medium is crushed under a pressure of 1000-1500 bar, and then centrifuged to obtain the supernatant to obtain the Bacillus fermentation product. The fermentation medium formula is: 18-22g carbon source, 8-12g nitrogen source, 0.4-0.6g sodium sulfate, 1.8-2.2g sodium dihydrogen phosphate, 0.08-0.12g calcium chloride, 0.18-0.22g magnesium sulfate, 0.48-0.52g sodium chloride, 0.48-0.52g sodium glutamate, and distilled water is added to 1000mL.

7. The mood beauty composition according to claim 6, characterized in that At least one of the following is met: (1) The Bacillus includes at least one of Bacillus marineus, Bacillus subtilis, Bacillus licheniformis, and Bacillus megaterium; (2) The carbon source includes at least one of oat extract, glucose, sucrose, and soybean extract; (3) The nitrogen source includes peptone; (4) The inoculation amount of the Bacillus is 10^9~10^10 CFU / mL.

8. Use of the mood beauty composition according to any one of claims 1 to 7 in the preparation of skin care products.

9. An emulsion, characterized in that The emulsion comprises the following components in mass percentage: 1-10% of the emotional beauty composition according to any one of claims 1 to 7, 0.05-0.3% of a thickener, 0.5-1% of a moisturizer, 0.01-0.3% of a pH regulator, 0.5-5% of an emulsifier, 0.5-3% of a preservative, and the balance deionized water.

10. The emulsion according to claim 9, characterized in that At least one of the following is met: (a) the thickener comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol; (b) the moisturizing agent comprises at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butylene glycol, glycerin, budding sclerotin polysaccharide, and ceramide; (c) the pH adjuster comprises at least one of arginine, tromethamine, and EDTA-disodium; (d) the emulsifier comprises at least one of caprylic capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetraisostearate, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate; (e) The preservative includes at least one of 1,2-hexanediol and p-hydroxyacetophenone.

Images