Anti-carsickness essential oil as well as preparation and application thereof

Abstract

The invention relates to the technical field of anti-carsickness essential oil, in particular to anti-carsickness essential oil as well as preparation and application thereof. The anti-carsickness essential oil comprises the following components in parts by weight: 1-5 parts of clove essential oil, 1-4 parts of pericarpium citri reticulatae essential oil, 1-3 parts of artemisia apiacea essential oil and 0-1 part of cinnamon oil. The invention aims to compound the safe and effective anti-carsickness essential oil with warm nature under the guidance of the monarch, minister, assistant and guide theory of traditional Chinese medicine in combination with modern western medicine pharmacology. The clove essential oil, the pericarpium citri reticulatae essential oil, the artemisia apiacea essential oil and the cinnamon oil are selected as main components to be compounded; the clove essential oil is used as a monarch drug and has the effects of warming middle-jiao, calming adverse-rising energy, tonifying kidney and supporting yang; the pericarpium citri reticulatae essential oil is used as a ministerial drug to assist the clove essential oil to enhance the effects of regulating qi and harmonizing stomach The artemisia apiacea essential oil is used as an adjuvant drug, balances the warm property of the formula, and has an anti-inflammatory effect; cinnamon oil is used as a conductant drug to guide other essential oils to play a better role and enhance the spleen-stomach warming and cold dispelling effects of the whole formula.

Description

Technical Field

[0001] The invention relates to the technical field of anti-motion sickness essential oil, in particular to anti-motion sickness essential oil and preparation and application thereof. Background Art

[0002] Motion sickness is a common motion sickness, which manifests as nausea, vomiting, dizziness and other symptoms when riding in public transportation. Traditional treatments include medication and traditional Chinese medicine, but there are certain limitations, such as drug side effects and unstable treatment effects. At present, the anti-motion sickness products on the market mainly include oral medications, patches, etc. Oral medications may cause side effects such as drowsiness and dry mouth, and patches are not breathable and can easily cause skin irritation. Therefore, it is of great practical significance to develop a safe, effective and side-effect-free anti-motion sickness method.

[0003] In TCM theory, the coordination of the functions of the organs of the human body is the basis for maintaining a normal physiological state. Motion sickness is mainly related to the spleen, stomach and liver. The spleen and stomach are the foundation of the acquired constitution and the source of qi and blood. When riding in a car or other means of transportation, the human body is in a bumpy state, and the spleen and stomach are easily affected. If the spleen and stomach are not rising and falling normally, and the stomach qi is reversed, motion sickness symptoms such as nausea and vomiting will occur. The liver is responsible for regulating qi. If emotional tension or physical factors cause liver qi discomfort, it will affect the transportation and transformation function of the spleen and stomach. For example, a person is highly nervous before riding a car, the liver fails to regulate qi, and then the spleen and stomach are suppressed, causing the spleen and stomach to be disordered, and motion sickness reactions are more likely to occur under stimulation such as vehicle shaking. Not only that, TCM also believes that the circulation of qi and blood in the human body has its own rules. Under normal circumstances, qi and blood circulate throughout the body to maintain the normal functions of various organs and tissues. When motion sickness occurs, due to external factors such as shaking, the circulation of qi and blood in the human body is disturbed and becomes chaotic. Disharmony of qi and blood in the head is also a manifestation of motion sickness. If the qi and blood rush to the head, it will cause dizziness; and if the qi and blood of the spleen and stomach are disordered, it will cause the stomach qi to rise instead of falling, resulting in symptoms such as nausea and vomiting. The disorder of qi and blood will also affect the mind, causing discomfort such as irritability and panic, because the heart is the master of the spirit, and the disorder of qi and blood will disturb the peace of mind. People with different physiques have different susceptibility to motion sickness: people with qi deficiency have insufficient positive energy and relatively weak qi and blood circulation. During the ride, it is more difficult for the body to adapt to changes in the external environment, and it is also easy to experience motion sickness symptoms; people with phlegm and dampness constitution have more phlegm and dampness in the body, which will hinder the transportation and transformation of the spleen and stomach and the rise and fall of qi. When stimulated by vehicle bumps and other stimuli, the internal obstruction of phlegm and dampness is aggravated, the spleen and stomach are out of order, and the symptoms of motion sickness such as nausea, vomiting, and dizziness will also frequently occur.

[0004] From the perspective of human structure, the human inner ear contains vestibular organs, which include semicircular canals, utricles and saccules. The semicircular canals mainly sense angular acceleration, such as the rotation of the head; the utricles and saccules mainly sense linear acceleration, such as the linear motion of the vehicle starting, stopping, accelerating and decelerating. When riding in a vehicle, the bumps, turns and other movements of the vehicle cause the vestibular organs to be stimulated beyond the normal adaptation range of the human body. For example, when driving on a rugged mountain road, the vehicle frequently shakes left and right and bumps up and down. The endolymph in the semicircular canals will flow due to inertia, stimulating the hair cells, which convert this mechanical stimulation into nerve impulses and transmit them to the brain through the vestibular nerves. If these nerve impulses do not match the information transmitted by the human body from other sensory organs such as vision and proprioception, the brain will receive confusing motion signals, causing motion sickness symptoms such as dizziness, nausea, vomiting, etc. The occurrence of motion sickness is also related to the regulation of the nervous system. Under normal circumstances, the central nervous system will integrate and regulate multiple sensory information from the vestibular, visual and proprioceptive senses. When subjected to excessive motion stimulation, the neural regulation function of the vestibular center may become unbalanced. When motion sickness occurs, the excessive excitement of the vestibular nerve nucleus will be transmitted to the vagus nerve nucleus through the nerve pathway. The excitement of the vagus nerve will cause gastrointestinal dysfunction, leading to nausea, vomiting and other reactions. At the same time, the nervous system will also affect the cardiovascular system, which may cause symptoms such as palpitations and pale complexion.

[0005] Patent CN107123456A discloses a motion sickness prevention essential oil formula, the main ingredients of which are peppermint essential oil, ginger essential oil and lavender essential oil, which relieve motion sickness symptoms by volatile aroma. The anti-motion sickness essential oil has the following disadvantages: the volatilization speed is fast, the aroma lasts for a short time, and it cannot meet the needs of long-term travel; it only relieves motion sickness symptoms through aroma, lacks direct regulation of the nervous system, and has limited effect.

[0006] Therefore, it is still crucial to provide an anti-motion sickness essential oil that can solve the above problems. Summary of the invention

[0007] In order to solve the above problems, the purpose of the present invention is to provide an anti-motion sickness essential oil and its preparation and application. The present invention achieves the effect of preventing and relieving motion sickness symptoms by scientifically proportioning a variety of natural plant essential oils, combining traditional Chinese medicine theory preparation and traditional pharmacology verification.

[0008] The purpose of the present invention can be achieved by the following technical solutions:

[0009] The first object of the present invention is to provide an anti-motion sickness essential oil, comprising the following components in parts by weight:

[0010] 1-5 parts of clove essential oil, 1-4 parts of tangerine peel essential oil, 1-3 parts of artemisia annua essential oil, 0-1 part of cinnamon oil;

[0011] Among them, clove essential oil is used as the main medicine, tangerine peel essential oil is used as the secondary medicine, Artemisia annua essential oil is used as the adjuvant medicine, and cinnamon oil is used as the guiding medicine.

[0012] Further, the following components are included in parts by weight:

[0013] 5 parts of clove essential oil, 1-4 parts of tangerine peel essential oil, 1-3 parts of artemisia annua essential oil, and 1 part of cinnamon oil.

[0014] Furthermore, the following components are included in parts by weight:

[0015] 5 parts of clove essential oil, 1 part of tangerine peel essential oil, 1 part of artemisia annua essential oil, and 1 part of cinnamon oil.

[0016] Furthermore, the following components are included in parts by weight:

[0017] 5 parts of clove essential oil, 4 parts of tangerine peel essential oil, 3 parts of artemisia annua essential oil, 1 part of cinnamon oil.

[0018] Further, the following components are included in parts by weight:

[0019] 1 part of clove essential oil, 1 part of tangerine peel essential oil, and 1 part of artemisia annua essential oil.

[0020] Further, the following components are included in parts by weight:

[0021] 3 parts of clove essential oil, 2 parts of tangerine peel essential oil, 1 part of artemisia annua essential oil.

[0022] Further, the following components are included in parts by weight:

[0023] 1 part of clove essential oil, 1 part of tangerine peel essential oil, 1 part of artemisia annua essential oil, and 1 part of cinnamon oil.

[0024] A second object of the present invention is to provide a method for preparing an anti-motion sickness essential oil, comprising the following steps:

[0025] Put clove essential oil, tangerine peel essential oil, artemisia annua essential oil and cinnamon oil in a brown glass bottle away from light and mix well to obtain anti-motion sickness essential oil;

[0026] Alternatively, clove essential oil, tangerine peel essential oil and artemisia annua essential oil are placed in a brown glass bottle away from light and mixed to obtain the anti-motion sickness essential oil.

[0027] In the present invention, when the anti-motion sickness essential oil is used directly, it can be dropped on a fragrance diffuser, a napkin or a cotton ball, and sniffed before riding or when feeling unwell, so as to achieve the effect of preventing and / or alleviating motion sickness.

[0028] The third object of the present invention is to provide an application of anti-motion sickness essential oil in the preparation of anti-motion sickness products.

[0029] In one embodiment of the present invention, the product is selected from one of a roller ball, a spray, an aromatherapy pendant, an ointment, an aromatherapy, an aromatherapy bracelet, an aromatherapy candle, an inhalation stick or a massage oil.

[0030] In the present invention, the essential oil is selected from natural plant essential oils with the effects of calming the nerves, stopping vomiting and resisting vertigo.

[0031] Clove is used to treat stomach cold, vomiting, and hiccups. It is pungent, warm, and aromatic. It can warm the spleen and stomach and relieve qi stagnation. It is especially good at lowering adverse qi. Therefore, it has the effects of warming the middle and dispersing cold, lowering adverse qi and stopping vomiting, and stopping hiccups. It is regarded as an important medicinal material for treating stomach cold, vomiting, and hiccups. In addition, clove can also treat cold pain in the abdomen because it can warm the middle and dispersing cold and stop pain. It can also treat impotence and cold uterus because it is pungent and warm in nature and enters the kidney meridian, which has the effect of warming the kidney and assisting yang to cure impotence. One of the theories of TCM interpretation of motion sickness may be related to "water stagnation" in the body, that is, the "water stagnation" that should have been transformed into gas accumulates in the body and rises in the shaking and bumping, causing dizziness and vomiting. Clove is warm in nature, can warm the middle and dispersing cold, help resolve "water stagnation", and has the effect of regulating qi and stomach, which can help harmonize the spleen and stomach and reduce the feeling of nausea and vomiting during motion sickness.

[0032] Tangerine peel is warm in nature, pungent and bitter in taste, and belongs to the spleen and lung meridians. It has the effects of regulating qi and strengthening the spleen, drying dampness and resolving phlegm. It can regulate the spleen and stomach qi, promote the secretion of digestive juices, eliminate gas accumulation in the intestines, increase appetite, and assist the main medicine in regulating gastrointestinal qi, enhancing gastrointestinal function, and alleviating symptoms such as nausea and vomiting. In addition, tangerine peel also has the effect of removing phlegm and relieving asthma. The limonene it contains can stimulate the bronchi, causing increased glandular secretion, making it easier to cough up phlegm.

[0033] Artemisia annua tastes bitter and slightly pungent, and is cold in nature. It is classified into the liver and gallbladder meridians. It is mainly used to treat symptoms such as febrile diseases, summer heat, bone steaming and fatigue fever, malaria, dysentery, jaundice, scabies, itching, etc. It may have a certain effect on relieving motion sickness symptoms such as dizziness and nausea caused by summer heat. Artemisia annua volatile oil has amber and orchid flower fragrance, which has an aromatic and orchid-opening effect, and may help relieve motion sickness nausea.

[0034] Cinnamon is hot in nature and can be harmonized with the artemisia annua essential oil in this formula. It tastes spicy and sweet. It enters the kidney meridian, spleen meridian, heart meridian, and liver meridian, and has the effects of replenishing fire and yang, guiding fire back to the origin, dispelling cold and relieving pain, and warming and unblocking the meridians. Cinnamon has a mild stimulating effect on the stomach and intestines, and can relieve gastrointestinal smooth muscle spasms, and has a strong anti-ulcer effect; it can antagonize platelet aggregation, improve the cardiovascular system, and regulate the body's immune function; it has sedative, analgesic, antipyretic, and anticonvulsant effects on the central nervous system; it has a preventive and protective effect on yang deficiency and yin deficiency models.

[0035] Essential oil ratio: monarch, minister, assistant and envoy is the basic principle of Chinese medicine prescription compatibility, among which the "monarch" medicine is the main medicine, targeting the main symptoms; the "minister" medicine assists the monarch medicine to enhance the efficacy or treat the concurrent symptoms; the "assistant" medicine assists the assistant medicine or inhibits the toxicity of the monarch and minister medicines; the "envoy" medicine guides the medicines directly to the diseased area or harmonizes the medicines. According to the monarch, minister, assistant and envoy theory of Chinese medicine and Western medicine pharmacology, the best ratio of each essential oil is determined to achieve the best therapeutic effect.

[0036] Based on the theory of monarch, minister, assistant and envoy in traditional Chinese medicine, clove essential oil, tangerine peel essential oil, artemisia annua essential oil and cinnamon oil are compounded to obtain a warm, safe and effective essential oil for the treatment of motion sickness:

[0037] Sovereign drug - clove essential oil (dominant): Clove essential oil is warm in nature and has the effects of warming the middle and lowering adverse qi, tonifying the kidney and supporting yang. In the treatment of motion sickness, clove essential oil can be used as the sovereign drug, and its main function is to warm the middle and dispel cold, and relieve spleen and stomach discomfort and vomiting caused by cold evil.

[0038] Assistant medicine - Tangerine peel essential oil (assistant to clove essential oil): Tangerine peel essential oil is warm in nature, pungent and bitter in taste, and enters the spleen and lung meridians. It has the effects of regulating qi and strengthening the spleen, drying dampness and resolving phlegm. As an assistant medicine, tangerine peel essential oil assists clove essential oil, enhancing its effect of regulating qi and harmonizing the stomach, and reducing the symptoms of nausea and vomiting.

[0039] Adjuvant - Artemisia annua essential oil (balanced overall formula): Artemisia annua essential oil is cold in nature, bitter and slightly pungent in taste, and enters the liver meridian and gallbladder meridian. It has the effects of clearing away heat and relieving summer heat, relieving deficiency heat, and eliminating bone steaming. As an adjuvant, Artemisia annua essential oil can balance the warming properties of clove essential oil and tangerine peel essential oil, prevent excessive warmth, and have anti-inflammatory effects.

[0040] Envoy - Cinnamon Oil (guides other essential oils to play a role): Cinnamon oil is very hot, pungent and sweet, and enters the kidney meridian, spleen meridian, heart meridian, and liver meridian. It has the effects of replenishing fire and yang, guiding fire back to the origin, and dispelling cold and relieving pain. As an envoy, cinnamon oil can guide other essential oils to play a better role. At the same time, its warming nature can assist clove essential oil and tangerine peel essential oil to enhance the warming and dispelling cold effect of the overall formula.

[0041] Compared with the prior art, the present invention has the following beneficial effects:

[0042] The present invention is guided by the theory of monarch, minister, assistant and messenger of traditional Chinese medicine, and cooperates with modern western medicine pharmacology to compound a warm, safe and effective anti-motion sickness essential oil. The anti-motion sickness essential oil of the present invention selects clove essential oil, tangerine peel essential oil, artemisia annua essential oil and cinnamon oil as main components for compounding. Among them, clove essential oil is used as monarch medicine to play its role of warming the middle and lowering adverse, tonifying the kidney and assisting yang; tangerine peel essential oil is used as minister medicine to assist clove essential oil in enhancing the effect of regulating qi and stomach; artemisia annua essential oil is used as adjuvant medicine to balance the warming property of the formula and has anti-inflammatory effect; cinnamon oil is used as messenger medicine to guide other essential oils to play a better role and enhance the warming and cold-dispersing effect of the overall formula. The anti-motion sickness essential oil of the present invention is verified to be safe through skin test, and shows good anti-motion sickness effect in practical application. The anti-motion sickness essential oil of the present invention not only has the advantages of traditional Chinese medicine, but also provides a new prevention and treatment option for motion sickness patients. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 Schematic diagram of the total movement path of mice in each group;

[0044] Figure 2 Schematic diagram of the number of wall climbing and standing of mice in each group;

[0045] Figure 3 Schematic diagram of the passage time of mice in each group;

[0046] Figure 4 Schematic diagram of the number of falls of mice in each group;

[0047] Figure 5 Schematic diagram of the contents of 5-HT, GABA, and NE in each group of mice. DETAILED DESCRIPTION

[0048] The present invention is described in detail below with reference to the accompanying drawings and specific embodiments.

[0049] In the following examples, clove essential oil, tangerine peel essential oil, and cinnamon oil were purchased from Shanghai Zixin Biotechnology Co., Ltd.; and artemisia annua essential oil was purchased from Lvyuan Natural Spice Oil Refinery, Qingyuan District, Ji'an City.

[0050] Unless otherwise specified, the reagents, methods, instruments and equipment used are commercially available reagents, conventional methods, instruments and equipment; the detection means and methods used are conventional detection means and methods in the field.

[0051] Example 1

[0052] This embodiment provides a method for preparing anti-motion sickness essential oil, which is as follows:

[0053] Weigh the following components in parts by weight: 1 part of clove essential oil, 1 part of tangerine peel essential oil, and 1 part of artemisia annua essential oil; mix them well and place them in a clean brown glass bottle away from light and stir to mix well, to obtain anti-motion sickness essential oil.

[0054] Example 2

[0055] This embodiment provides a method for preparing anti-motion sickness essential oil, which is as follows:

[0056] Weigh the following components in parts by weight: 3 parts of clove essential oil, 2 parts of tangerine peel essential oil, and 1 part of artemisia annua essential oil; mix them well and place them in a clean brown glass bottle away from light and stir to mix well, to obtain anti-motion sickness essential oil.

[0057] Example 3

[0058] This embodiment provides a method for preparing anti-motion sickness essential oil, which is as follows:

[0059] Weigh the following components in parts by weight: 1 part of clove essential oil, 1 part of tangerine peel essential oil, 1 part of artemisia annua essential oil, and 1 part of cinnamon essential oil; mix well and place in a clean brown glass bottle away from light and stir to mix well to obtain anti-motion sickness essential oil.

[0060] Example 4

[0061] This embodiment provides a method for preparing anti-motion sickness essential oil, which is as follows:

[0062] Weigh the following components in parts by weight: 5 parts of clove essential oil, 1 part of tangerine peel essential oil, 1 part of artemisia annua essential oil, and 1 part of cinnamon essential oil; mix them well and place them in a clean brown glass bottle away from light and stir to mix well to obtain anti-motion sickness essential oil.

[0063] Example 5

[0064] This embodiment provides a method for preparing anti-motion sickness essential oil, which is as follows:

[0065] Weigh the following components in parts by weight: 5 parts of clove essential oil, 4 parts of tangerine peel essential oil, 3 parts of artemisia annua essential oil, and 1 part of cinnamon essential oil; mix well and place in a clean brown glass bottle away from light and stir to obtain anti-motion sickness essential oil.

[0066] Pharmacological evaluation: The anti-motion sickness essential oil, clove essential oil, tangerine peel essential oil, artemisia annua essential oil, and cinnamon essential oil prepared in Examples 1 to 5 were subjected to the following animal behavior test (with the blank group and the model group as controls):

[0067] Experimental Animals:

[0068] (1) Animal selection and breeding:

[0069] Healthy SPF-grade ICR mice, male, 6-8 weeks old, weighing 27-33 g, were purchased from Shanghai Jiesijie Experimental Animal Co., Ltd., with the animal certificate number SCXK (Shanghai) 2023-0004. The animals were raised in an SPF environment, with a temperature of 22-25 °C, a humidity of 40%-70%, and a photoperiod of 12 h.

[0070] The experimental animals were randomly divided into a blank group, a model group, a clove essential oil group, a tangerine peel essential oil group, an artemisia annua essential oil group, a cinnamon essential oil group, an example 1 group, an example 2 group, an example 3 group, an example 4 group, and an example 5 group, with 8 animals in each group.

[0071] Among them, the mice in the blank group were raised normally and no motion sickness model was established, while the mice in the other groups were all established with motion sickness models; the motion sickness group was given distilled water, and the other groups were given corresponding essential oils.

[0072] Motion sickness model establishment: During the rotation process, the visual, vestibular, and proprioceptive receptors of animals interact with each other, and the sympathetic nerves are excited, causing a series of physiological changes such as piloerection, tremor, incontinence, and pica. Mice were placed in a 50mL centrifuge tube with cotton pads around and at the bottom, and centrifuged at 250r / min in a low-speed centrifuge for 5 minutes.

[0073] Administration method: Drop anti-motion sickness essential oil on a cotton ball, put it in the mouse's cage, and let it naturally inhale the aroma of the essential oil for 5 minutes.

[0074] 5 minutes after administration, the animals were placed in the simulated exercise device and the device was turned on. During the exercise, the behavioral changes of the animals were observed, including the animals' mobility and balance abilities.

[0075] After the exercise, behavioral experiments were immediately carried out and compared with the blank group and motion sickness group.

[0076] Effectiveness evaluation:

[0077] 1) Behavioral indicators: The open field experiment aims to observe the neuropsychiatric status and various behavioral manifestations of experimental animals in an open environment, and is particularly suitable for studying animal reactions in dizzy situations. The specific operation is to place the dizzy mice in an open field box and record their activity frequency within 5 minutes. Given that the mice have reduced activity in a dizzy state, this is similar to the mental depression and slow reaction characteristics of humans when they are dizzy, and thus the changes in their central nervous system can be indirectly inferred, so it can be used as a unique indicator to determine the onset of motion sickness in mice.

[0078] 2) Balance beam experiment: Motion sickness can affect the balance ability of mice, and the balance beam experiment can well detect the movement coordination and balance function of mice. Set up a balance beam with a length of 1m and a width of 2cm, place the mouse at one end of the balance beam, and put sweet food at the other end to induce the mouse to move forward, and let it pass the balance beam to the other end by itself. Record the time it takes for the mouse to pass the balance beam, the number of times it falls, and other indicators.

[0079] 3) Physiological indicators: Existing studies have fully demonstrated that the vestibular system not only has the basic functions of receiving, integrating and regulating vestibular information, but also can organically integrate the incoming information from the vestibular organs, visual system and proprioceptive system, thereby maintaining the body's sense of balance and spatial orientation. In this complex central vestibular function regulation network, monoamine neurotransmitters play an indispensable and key role. As an important neural signal transmission medium, they are directly involved in the fine regulation of vestibular function. From the perspective of clinical treatment, most of the therapeutic drugs currently used for motion sickness vomiting and vestibular dysfunction are based on monoamine neurotransmitter receptor agonists or antagonists as the main ingredients, such as betahistine, which regulates the release of monoamine neurotransmitters such as norepinephrine, thereby alleviating related symptoms. This also indirectly confirms the important position of monoamine neurotransmitters in vestibular function regulation. Based on this theoretical background, in the present invention, the researchers accurately measured the changes in the content of key monoamine neurotransmitters such as serotonin (5-HT), γ-aminobutyric acid (GABA), and norepinephrine (NE) in the mouse brain, and used this as an important basis for judging whether the essential oils studied have an effective effect in preventing motion sickness. The purpose is to deeply explore the potential impact mechanism of essential oils on vestibular function from the neurotransmitter level.

[0080] The results of the above tests are as follows:

[0081] Table 1 Behavioral results of mice in the mine test

[0082] Total movement distance (mm) Wall Stands blank 15994.7263±3788.33774 80.125±13.95337 Model 5846.4525±1032.70452 48.25±11.79285 Clove essential oil 9746.7625±399.60362 66.25±10.81996 Tangerine peel essential oil 8983.2725±1694.30958 59±7.63451 Artemisia annua essential oil 7197.7363±1457.30897 56.5±5.8064 Cinnamon Oil 6779.9213±1313.19910 51.625±7.96308 Example 1 9780.3013±1134.19614 68.875±12.06456 Example 2 10478.1787±2051.01158 71.25±9.43777 Example 3 10668.3925±2216.6019 78.25±9.52815 Example 4 11593.55±2249.87654 80.75±10.16647 Example 5 15293.0488±4942.90175 86.25±8.84388

[0083] The behavioral results of mice in the open field test are shown in Table 1 and Figures 1-2 As shown, the results showed that the total movement distance and the number of wall climbing and standing in the open field test of the model group mice were significantly lower than those of the control group (P<0.05), indicating that the autonomous activity ability of the model group mice was reduced. The behavioral performance of the mice in the open field of each single essential oil and the implemented essential oil group was improved to varying degrees compared with the model group, and the improvement effect of each embodiment was better than that of the single essential oil, which illustrates the necessity of the compounding of essential oils. Among them, the anti-motion sickness essential oil prepared by Example 5 was found to have the best recovery effect on the autonomous activity of the open field test of mice, indicating that sniffing the anti-motion sickness essential oil of Example 5 significantly improved the motion sickness symptoms of mice.

[0084] Table 2 Behavioral results of mice in the balance beam test

[0085] Passing time(s) Number of drops blank 10.4438±4.72121 0.25±0.46291 Model 45.32±14.98894 3±1.19523 Clove essential oil 33.1837±9.91156 1.25±0.88641 Tangerine peel essential oil 34.9112±10.14541 1.5±0.53452 Artemisia annua essential oil 36.2388±6.28971 2.25±0.88641 Cinnamon Oil 40.5475±9.33539 2.375±0.74402 Example 1 30.7788±9.82852 1.375±0.74402 Example 2 29.4675±14.77906 1±0.53452 Example 3 28.7988±8.36751 0.875±0.83452 Example 4 21.595±3.9281 0.375±0.51755 Example 5 13.585±6.1617 0.25±0.46291

[0086] The behavioral results of mice in the balance beam test are shown in Table 2 and Figures 3-4 As shown, the time taken by the mice in the model group to pass the balance beam and the number of times they fell from the balance beam were significantly higher than those in the model group (P<0.05), indicating a decrease in the balance ability of the mice in the model group, indicating that the motion sickness model was successfully established. The behavior of the mice in the single essential oil group (clove essential oil group, tangerine peel essential oil group, artemisia annua essential oil group, cinnamon essential oil group) and the anti-motion sickness essential oil group prepared in Examples 1 to 5 on the balance beam was improved to varying degrees compared with the model group, and there was a certain correlation between the passing time and the number of falls, but there were also individuals with a long passing time and a small number of falls. Among them, the degree of recovery of the movement of the mice in the balance beam test in Example 5 group was higher than that in other examples, which illustrates the advantages of the anti-motion sickness essential oil obtained according to the theory of monarch, minister, assistant and envoy in traditional Chinese medicine.

[0087] Table 3 Contents of monoamine neurotransmitters in mouse brain tissue

[0088] 5-HT (pg / mL) GABA(pg / mL) NE (pg / mL) blank 200.7201±14.53289 9.6329±0.82643 7.2186±0.35681 Model 104.4439±14.74202 5.4483±1.14991 3.6444±0.20469 Clove essential oil 139.0529±10.15082 7.2886±1.76938 5.3368±0.27887 Tangerine peel essential oil 117.9139±19.70443 6.7427±1.3151 4.8148±0.37828 Artemisia annua essential oil 112.2994±10.61298 6.0237±0.30036 4.629±1.08633 Cinnamon Oil 108.5336±25.84631 6.0608±1.58779 4.0975±0.34045 Example 1 154.6059±31.03404 7.3923±1.17917 5.5745±0.23731 Example 2 158.4809±48.60191 7.8156±0.8072 5.9946±0.81173 Example 3 163.4005±37.56014 7.8319±1.96497 6.3926±0.3108 Example 4 181.1773±11.84905 8.0765±1.65114 6.6076±0.43877 Example 5 203.0733±26.40518 10.3831±1.05205 7.1078±0.73244

[0089] As shown in Table 3 and Figure 5 As shown in the figure, compared with the blank group, the contents of 5-HT, GABA, and NE in the brain tissues of the other motion sickness model groups except the model group were significantly reduced (P<0.05). Compared with the model group, the 5-HT, GABA, and NE of the anti-motion sickness essential oil of Example 5 after administration have basically returned to the levels of normal mice, indicating that the anti-motion sickness essential oil can not only improve the motion sickness behavior of mice, but also enter the brain through the nasal cavity to exert its effects.

[0090] In addition, the results of the determination of behavioral and in vivo indicators show that the anti-motion sickness essential oils prepared in the above Examples 1 to 5 have a certain preventive and therapeutic effect on motion sickness in mice. Through the comparison between the five examples, it can be seen that the effect of the essential oil prepared according to the theory of monarch, minister, assistant and messenger in traditional Chinese medicine (Example 5 group) is better than the lack of a certain component essential oil (Example 1 group, Example 2 group), the equal proportion of essential oil (Example 3 group) and the single main component essential oil (Example 4 group). This is because the monarch, minister, assistant and messenger theory is like an organized team, the monarch medicine is the core, and plays a key therapeutic role for the main symptoms; the minister medicine assists the monarch medicine to strengthen the role of treating the main disease or main syndrome; the adjuvant medicine plays a role from both positive and negative aspects, the adjuvant medicine assists the monarch and minister medicine to enhance the efficacy, and the adjuvant medicine restricts the toxicity or potency of the monarch and minister medicine; the messenger medicine plays the role of reconciling the various medicines or guiding the meridians and reporting the messenger. This systematic compatibility can comprehensively consider the relationship between the condition and the medicine (there is a synergistic effect between the components) and solve the problem of motion sickness from multiple angles.

[0091] The above description of the embodiments is to facilitate the understanding and use of the invention by those skilled in the art. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the present invention is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the explanation of the present invention without departing from the scope of the present invention should be within the scope of protection of the present invention.

Claims

1. An anti-motion sickness essential oil, characterized in that: The composition comprises the following components in parts by weight: 1-5 parts of clove essential oil, 1-4 parts of tangerine peel essential oil, 1-3 parts of artemisia annua essential oil, 0-1 part of cinnamon oil; Among them, clove essential oil is used as the main medicine, tangerine peel essential oil is used as the secondary medicine, Artemisia annua essential oil is used as the adjuvant medicine, and cinnamon oil is used as the guiding medicine.

2. The anti-motion sickness essential oil according to claim 1, characterized in that: The composition comprises the following components in parts by weight: 5 parts of clove essential oil, 1-4 parts of tangerine peel essential oil, 1-3 parts of artemisia annua essential oil, and 1 part of cinnamon oil.

3. The anti-motion sickness essential oil according to claim 2, characterized in that The composition comprises the following components in parts by weight: 5 parts of clove essential oil, 1 part of tangerine peel essential oil, 1 part of artemisia annua essential oil, and 1 part of cinnamon oil.

4. The anti-motion sickness essential oil according to claim 2, characterized in that The composition comprises the following components in parts by weight: 5 parts of clove essential oil, 4 parts of tangerine peel essential oil, 3 parts of artemisia annua essential oil, 1 part of cinnamon oil.

5. The anti-motion sickness essential oil according to claim 1, characterized in that: The composition comprises the following components in parts by weight: 1 part of clove essential oil, 1 part of tangerine peel essential oil, and 1 part of artemisia annua essential oil.

6. The anti-motion sickness essential oil according to claim 1, characterized in that: The composition comprises the following components in parts by weight: 3 parts of clove essential oil, 2 parts of tangerine peel essential oil, 1 part of artemisia annua essential oil.

7. The anti-motion sickness essential oil according to claim 1, characterized in that: The composition comprises the following components in parts by weight: 1 part of clove essential oil, 1 part of tangerine peel essential oil, 1 part of artemisia annua essential oil, and 1 part of cinnamon oil.

8. A method for preparing the anti-motion sickness essential oil according to any one of claims 1 to 7, characterized in that: The following steps are involved: Put clove essential oil, tangerine peel essential oil, artemisia annua essential oil and cinnamon oil in a brown glass bottle away from light and mix well to obtain anti-motion sickness essential oil; Alternatively, clove essential oil, tangerine peel essential oil and artemisia annua essential oil are placed in a brown glass bottle away from light and mixed to obtain the anti-motion sickness essential oil.

9. Use of the anti-motion sickness essential oil according to any one of claims 1 to 7 in the preparation of an anti-motion sickness product.

10. The use according to claim 9, characterized in that: The product is selected from one of a roller ball, a spray, an aromatherapy pendant, an ointment, an aromatherapy, an aromatherapy bracelet, an aromatherapy candle, an inhalation stick or a massage oil.

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