Vitamin D-rich shiny-leaved yellowhorn oil powder grease and preparation method thereof

Abstract

The invention relates to the technical field of pharmaceutical preparations, and provides shiny-leaved yellowhorn oil powder grease rich in vitamin D and a preparation method thereof. The preparation method comprises the following steps: weighing a certain amount of shiny-leaved yellowhorn oil and vitamin D, and mixing to obtain a mixed solution; carrying out rotary evaporation on the mixed solution until the mixed solution forms a uniform thin film; washing the film with a phosphate buffer solution, and hydrating for 2 hours to obtain a crude suspension; performing ultrasonic treatment on the coarse suspension in an ice-water bath to obtain a suspension; dissolving sodium caseinate and maltooligosaccharide in a phosphate buffer solution, adding the suspension and an emulsifier, and mixing for 1 hour to obtain an emulsion; and carrying out spray drying treatment on the emulsion. The xanthoceras sorbifolia bunge oil and the vitamin D are mixed, the xanthoceras sorbifolia bunge oil powder grease is prepared through spray drying, the oxidation rate of the vitamin D and the xanthoceras sorbifolia bunge oil can be reduced, the absorption rate can be increased, and the sleeping effect is jointly enhanced by the vitamin D and nervonic acid in the xanthoceras sorbifolia bunge oil.

Description

Technical Field

[0001] The invention belongs to the technical field of pharmaceutical preparations, and in particular relates to Xanthoceras sorbifolia oil powder grease rich in vitamin D and a preparation method thereof. Background Art

[0002] Milk allergy refers to a series of clinical symptoms caused by the body's excessive immune response to milk protein after one or several protein molecules in milk protein enter the blood. Symptoms of milk allergy include severe allergic reactions, which can manifest as rashes on the body, or even swelling of the throat and face. With the continuous development of modern medicine, the understanding and treatment of milk allergies are also gradually deepening. Studies have found that milk allergies may be accompanied by insomnia symptoms for some reasons. For example, the immune response of patients with milk allergies to milk protein may cause interference with the nervous system, thereby affecting sleep; patients are troubled by allergic symptoms for a long time, and have emotional problems such as psychological anxiety, which indirectly leads to insomnia.

[0003] The existing methods for treating milk allergy mainly include avoiding the intake of milk and milk products, drug treatment and desensitization therapy, etc., all of which have certain limitations. Drug treatment includes the use of compound formula of vitamin D for treatment, which can alleviate the symptoms of dairy allergy by enhancing intestinal health, but has limited effect on specific populations (such as those who are prone to allergies) and cannot meet the needs of different individuals; and the preparation process of compound formula of vitamin D is relatively complicated, involving the ratio of multiple ingredients, and the cost is relatively high. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention provides a vitamin D-rich Xanthoceras sorbifolia oil powder and a preparation method thereof, the purpose of which is to solve the problems mentioned in the background technology.

[0005] Xanthoceras sorbifolia oil is an oil extracted from Xanthoceras sorbifolia. It is easily oxidized or degraded due to its high unsaturated fatty acid content of 94%, and has a low absorption rate. In addition, Xanthoceras sorbifolia oil also contains 3%-5% neuraminic acid, which is a core natural component of brain nerve fibers and nerve cells. It can repair and clear damaged brain nerve fibers and promote the regeneration of nerve cells.

[0006] In a first aspect, the present invention provides a method for preparing Xanthoceras sorbifolia oil powdered fat rich in vitamin D, comprising the following steps: Step S1: weighing a certain amount of Xanthoceras sorbifolia oil and vitamin D and mixing them to obtain a mixed solution; Step S2: adding the mixed solution into a round-bottom flask and performing rotary evaporation until the mixed solution forms a uniform thin film on the inner wall of the round-bottom flask; Step S3: washing the film with phosphate buffer and hydrating it for 2 hours at room temperature to obtain a crude suspension; Step S4: ultrasonically treating the crude suspension in an ice-water bath to obtain a uniformly dispersed suspension; Step S5: dissolving sodium caseinate and maltooligosaccharide in phosphate buffer, adding the suspension and emulsifier, and mixing in a high shear mixer for 1 hour to obtain a fully mixed emulsion; Step S6: sending the emulsion into a spray dryer for spray drying to obtain Xanthoceras sorbifolia oil powder.

[0007] Furthermore, in step S1, the mass percentage of Xanthoceras sorbifolia oil weighed is 99.0%-99.5% and the mass percentage of vitamin D weighed is 0.5%-1.0%.

[0008] Furthermore, in step S2, the temperature of the rotary evaporation is 45°C.

[0009] Furthermore, in step S4, the ultrasonic power is 300 W and the ultrasonic treatment time is 10 minutes.

[0010] Furthermore, in step S5, the mass ratio of sodium caseinate to maltooligosaccharide is 2:1.

[0011] Furthermore, in step S5, sodium caseinate and maltooligosaccharide are dissolved in phosphate buffer at a solid-liquid ratio of 1 g:5-10 mL; and the emulsifier is sucrose lipid.

[0012] Furthermore, in step S5, the rotation speed of the high shear mixer is 10000 rpm.

[0013] Furthermore, in step S6, the inlet temperature of the spray dryer is 150°C-160°C, and the outlet temperature of the spray dryer is 60°C.

[0014] Furthermore, in step S6, the flow rate of the emulsion fed into the spray dryer is 1.8 m³ / min.

[0015] In a second aspect, the present invention also provides a powdered fat of Xanthoceras sorbifolia oil rich in vitamin D.

[0016] The present invention has the following technical effects: (1) Xanthoceras sorbifolia oil and vitamin D are mixed and then spray-dried to prepare Xanthoceras sorbifolia oil powder. The Xanthoceras sorbifolia oil powder is a Xanthoceras sorbifolia oil microcapsule rich in vitamin D, which can reduce the oxidation rate of vitamin D and Xanthoceras sorbifolia oil and improve the absorption rate of vitamin D and Xanthoceras sorbifolia oil. The surface water content and oil content of the Xanthoceras sorbifolia oil powder are both less than 1%, the total oil content of Xanthoceras sorbifolia is ≥30%, and the embedding rate is greater than 99%. In the preparation process, the microcapsule wall material uses a mixture of sodium caseinate and oligomaltosan, which is a dense, fast-dissolving, and hard microcapsule wall material. Sodium caseinate can provide strong film-forming ability and stability, while oligomaltosan can increase the flexibility and heat resistance of the microcapsule. This combination allows the microcapsule to maintain its structural integrity under harsh environments such as temperature and pH.

[0017] (2) Xanthoceras sorbifolia oil, as a carrier, can improve the stability and bioavailability of vitamin D, effectively protect vitamin D from oxidation, and enhance the release of vitamin D in the body. The shelf life is more than 2 years. The vitamin D embedded in Xanthoceras sorbifolia oil and the nervonic acid in Xanthoceras sorbifolia oil work together to enhance the sleep effect, ultimately alleviating cow's milk allergy and the insomnia symptoms associated with cow's milk allergy. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] A more complete understanding of exemplary embodiments of the present invention may be obtained by referring to the following drawings: Figure 1 The present invention provides a flowchart of a method for preparing Xanthoceras sorbifolia oil powder rich in vitamin D provided in an embodiment of the present invention.

[0019] Figure 2 This is a diagram of the KEGG enrichment analysis results of vitamin D after embedding provided in Example 1 of the present invention. DETAILED DESCRIPTION

[0020] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0021] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs; the terms used herein are only for the purpose of describing specific embodiments rather than limiting the present invention.

[0022] The flow chart of the preparation method of Xanthoceras sorbifolia oil powder oil rich in vitamin D is as follows Figure 1 As shown, the embodiment of the present invention provides a method for preparing Xanthoceras sorbifolia oil powdered fat rich in vitamin D, comprising the following steps: Step S1: weighing a certain amount of Xanthoceras sorbifolia oil and vitamin D and mixing them to obtain a mixed solution; Step S2: adding the mixed solution into a round-bottom flask and performing rotary evaporation until the mixed solution forms a uniform thin film on the inner wall of the round-bottom flask; Step S3: washing the film with phosphate buffer and hydrating it for 2 hours at room temperature to obtain a crude suspension; Step S4: ultrasonically treating the crude suspension in an ice-water bath to obtain a uniformly dispersed suspension; Step S5: dissolving sodium caseinate and maltooligosaccharide in phosphate buffer, adding the suspension and emulsifier, and mixing in a high shear mixer for 1 hour to obtain a fully mixed emulsion; Step S6: sending the emulsion into a spray dryer for spray drying to obtain Xanthoceras sorbifolia oil powder.

[0023] Specifically, in step S1, the mass percentage of Xanthoceras sorbifolia oil weighed is 99.0%-99.5% and the mass percentage of vitamin D weighed is 0.5%-1.0%.

[0024] Specifically, in step S2, the temperature of the rotary evaporation is 45°C.

[0025] Specifically, in step S4, the ultrasonic power is 300 W and the ultrasonic treatment time is 10 minutes.

[0026] Specifically, in step S5, the mass ratio of sodium caseinate to maltooligosaccharide is 2:1.

[0027] Specifically, in step S5, sodium caseinate and maltooligosaccharide are dissolved in phosphate buffer at a solid-liquid ratio of 1 g:5-10 mL; and the emulsifier is sucrose lipid.

[0028] Specifically, in step S5, the rotation speed of the high shear mixer is 10000 rpm.

[0029] Specifically, in step S6, the inlet temperature of the spray dryer is 150°C-160°C, and the outlet temperature of the spray dryer is 60°C.

[0030] Specifically, in step S6, the flow rate of the emulsion fed into the spray dryer is 1.8 m³ / min.

[0031] The embodiment of the present invention also provides a vitamin D-rich Xanthoceras sorbifolia oil powder.

[0032] Embodiment 1: 99.5 g of Xanthoceras sorbifolia oil and 0.5 g of vitamin D were weighed to obtain a mixed solution; the mixed solution was added to a round-bottom flask, and rotary evaporated at a temperature of 45 °C until the mixed solution formed a uniform film on the inner wall of the round-bottom flask; the film was rinsed with phosphate buffer (pH=7.4), and hydrated for 2 hours at room temperature to obtain a coarse suspension; the coarse suspension was ultrasonically treated at 300 W for 10 minutes in an ice-water bath to obtain a uniformly dispersed suspension; 30 g of sodium caseinate and 15 g of oligomaltose were dissolved in 225 mL of phosphate buffer (pH=7.0), and the suspension and 0.5 g of sucrose lipid were added, and the mixture was placed in a high shear mixer and mixed for 1 hour to obtain a fully mixed emulsion; the emulsion was sent to a spray dryer at a flow rate of 1.8 m³ / min for spray drying, the inlet temperature of the spray dryer was 160 °C, and the outlet temperature of the spray dryer was 60 °C to obtain Xanthoceras sorbifolia oil powder.

[0033] The results of KEGG enrichment analysis after vitamin D embedding are as follows Figure 2 As shown in Figure 2, KEGG enrichment analysis mainly focuses on biological metabolic pathways and their interactions. Figure 2 Shown are: the top 20 pathways related to vitamin D after encapsulation, one of which is classified as a human disease, namely neuroactive ligand-receptor interaction, and has a high significance level; five pathways are classified as environmental information processing, eight pathways are classified as biological systems, and six pathways are classified as metabolism; and the KEGG pathways related to nerves mainly include: butyrate metabolism, phototransduction and antifolate pathways.

[0034] Embodiment 2: 99.3 g of Xanthoceras sorbifolia oil and 0.7 g of vitamin D were weighed to obtain a mixed solution; the mixed solution was added to a round-bottom flask, and rotary evaporated at a temperature of 45 °C until the mixed solution formed a uniform film on the inner wall of the round-bottom flask; the film was rinsed with phosphate buffer (pH=7.4), and hydrated for 2 hours at room temperature to obtain a coarse suspension; the coarse suspension was ultrasonically treated at 300 W for 10 minutes in an ice-water bath to obtain a uniformly dispersed suspension; 30 g of sodium caseinate and 15 g of oligomaltose were dissolved in 450 mL of phosphate buffer (pH=7.0), and the suspension and 0.8 g of sucrose lipid were added, and the mixture was placed in a high shear mixer and mixed for 1 hour to obtain a fully mixed emulsion; the emulsion was sent to a spray dryer at a flow rate of 1.8 m³ / min for spray drying, the inlet temperature of the spray dryer was 160 °C, and the outlet temperature of the spray dryer was 60 °C to obtain Xanthoceras sorbifolia oil powder.

[0035] Embodiment 3: 99.0 g of Xanthoceras sorbifolia oil and 1.0 g of vitamin D were weighed to obtain a mixed solution; the mixed solution was added to a round-bottom flask, and rotary evaporated at a temperature of 45 °C until the mixed solution formed a uniform film on the inner wall of the round-bottom flask; the film was rinsed with phosphate buffer (pH=7.4), and hydrated for 2 hours at room temperature to obtain a coarse suspension; the coarse suspension was ultrasonically treated at 300 W for 10 minutes in an ice-water bath to obtain a uniformly dispersed suspension; 30 g of sodium caseinate and 15 g of oligomaltose were dissolved in 300 mL of phosphate buffer (pH=7.0), and the suspension and 0.5 g of sucrose lipid were added, and the mixture was placed in a high shear mixer and mixed for 1 hour to obtain a fully mixed emulsion; the emulsion was sent to a spray dryer at a flow rate of 1.8 m³ / min for spray drying, the inlet temperature of the spray dryer was 150 °C, and the outlet temperature of the spray dryer was 60 °C to obtain Xanthoceras sorbifolia oil powder.

[0036] Comparative Example 1: 99.5 g of Xanthoceras sorbifolia oil and 0.5 g of vitamin D were weighed to obtain a mixed solution; the mixed solution was added to a round-bottom flask, and rotary evaporated at a temperature of 45 °C until the mixed solution formed a uniform film on the inner wall of the round-bottom flask; the film was rinsed with phosphate buffer (pH=7.4), and hydrated for 2 hours at room temperature to obtain a coarse suspension; the coarse suspension was ultrasonically treated at 300 W for 10 minutes in an ice-water bath to obtain a uniformly dispersed suspension; 30 g of sodium caseinate and 15 g of oligomaltose were dissolved in 225 mL of phosphate buffer (pH=7.0), and the suspension and 0.5 g of Tween-80 were added, and the mixture was placed in a high shear mixer and mixed for 1 hour to obtain a fully mixed emulsion; the emulsion was sent to a spray dryer at a flow rate of 1.8 m³ / min for spray drying, the inlet temperature of the spray dryer was 160 °C, and the outlet temperature of the spray dryer was 60 °C to obtain Xanthoceras sorbifolia oil powder.

[0037] Comparative Example 2: 99.3 g of Xanthoceras sorbifolia oil and 0.7 g of vitamin D were weighed to obtain a mixed solution; the mixed solution was added to a round-bottom flask, and rotary evaporated at a temperature of 45 °C until the mixed solution formed a uniform film on the inner wall of the round-bottom flask; the film was rinsed with phosphate buffer (pH=7.4), and hydrated for 2 hours at room temperature to obtain a coarse suspension; the coarse suspension was ultrasonically treated at 300 W for 10 minutes in an ice-water bath to obtain a uniformly dispersed suspension; 30 g of sodium caseinate and 15 g of oligomaltose were dissolved in 450 mL of phosphate buffer (pH=7.0), and the suspension and 0.8 g of sucrose lipid were added, and the mixture was placed in a high shear mixer and mixed for 1 hour to obtain a fully mixed emulsion; the emulsion was sent to a spray dryer at a flow rate of 1.8 m³ / min for spray drying, the inlet temperature of the spray dryer was 180 °C, and the outlet temperature of the spray dryer was 60 °C to obtain Xanthoceras sorbifolia oil powder.

[0038] The quality of the Xanthoceras sorbifolia oil powder obtained in Examples 1-3 and Comparative Examples 1-2 was measured, including the surface water content, surface oil content, embedding rate and total oil content of the Xanthoceras sorbifolia oil powder, as shown in Table 1.

[0039] Table 1 Quality of Xanthoceras sorbifolia oil powder

[0040] It can be seen from Table 1 that compared with Comparative Example 1 (emulsifier is Tween-80), Example 1 has lower surface water content and surface oil content, and higher total oil content and embedding rate of Xanthoceras sorbifolia; compared with Comparative Example 2 (the inlet temperature of the spray dryer is 180°C), Example 2 has lower surface water content and surface oil content, and higher total oil content and embedding rate of Xanthoceras sorbifolia.

[0041] In summary, Xanthoceras sorbifolia oil and vitamin D are mixed and then spray-dried to prepare Xanthoceras sorbifolia oil powder. Xanthoceras sorbifolia oil powder is a Xanthoceras sorbifolia oil microcapsule rich in vitamin D, which can reduce the oxidation rate of vitamin D and Xanthoceras sorbifolia oil and improve the absorption rate of vitamin D and Xanthoceras sorbifolia oil. The surface water content and oil content of Xanthoceras sorbifolia oil powder are both less than 1%, the total oil content of Xanthoceras sorbifolia is ≥30%, and the embedding rate is greater than 99%. In the preparation process, the microcapsule wall material uses a mixture of sodium caseinate and oligomaltosan, which is a dense, fast-dissolving, and hard microcapsule wall material. Sodium caseinate can provide strong film-forming ability and stability, while oligomaltosan can increase the flexibility and heat resistance of the microcapsule. This combination allows the microcapsule to maintain its structural integrity under harsh environments such as temperature and pH.

[0042] As a carrier, Xanthoceras sorbifolia oil can improve the stability and bioavailability of vitamin D, effectively protect vitamin D from oxidation, and enhance the release of vitamin D in the body. It has a shelf life of more than 2 years. The vitamin D embedded in Xanthoceras sorbifolia oil and the nervonic acid in Xanthoceras sorbifolia oil work together to enhance the sleep effect, ultimately alleviating cow's milk allergy and the insomnia symptoms associated with cow's milk allergy.

[0043] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for preparing Xanthoceras sorbifolia oil powder grease rich in vitamin D, characterized in that: The following steps are involved: Step S1: weighing a certain amount of Xanthoceras sorbifolia oil and vitamin D and mixing them to obtain a mixed solution; Step S2: adding the mixed solution into a round-bottom flask and performing rotary evaporation until the mixed solution forms a uniform thin film on the inner wall of the round-bottom flask; Step S3: washing the film with phosphate buffer and hydrating it for 2 hours at room temperature to obtain a crude suspension; Step S4: ultrasonically treating the crude suspension in an ice-water bath to obtain a uniformly dispersed suspension; Step S5: dissolving sodium caseinate and maltooligosaccharide in phosphate buffer, adding the suspension and emulsifier, and mixing in a high shear mixer for 1 hour to obtain a fully mixed emulsion; Step S6: sending the emulsion into a spray dryer for spray drying to obtain Xanthoceras sorbifolia oil powder.

2. The method for preparing a vitamin D-rich Xanthoceras sorbifolia oil powdered fat as claimed in claim 1, characterized in that: In step S1, the mass percentage of Xanthoceras sorbifolia oil weighed is 99.0%-99.5% and the mass percentage of vitamin D weighed is 0.5%-1.0%.

3. The method for preparing a vitamin D-rich Xanthoceras sorbifolia oil powdered fat as claimed in claim 2, characterized in that: In step S2, the temperature of the rotary evaporation is 45°C.

4. The method for preparing a vitamin D-rich Xanthoceras sorbifolia oil powdered fat as claimed in claim 3, characterized in that: In step S4, the ultrasonic power is 300 W and the ultrasonic treatment time is 10 minutes.

5. The method for preparing a vitamin D-rich Xanthoceras sorbifolia oil powdered fat as claimed in claim 4, characterized in that: In step S5, the mass ratio of sodium caseinate to maltooligosaccharide is 2:

1.

6. The method for preparing a vitamin D-rich Xanthoceras sorbifolia oil powdered fat as claimed in claim 5, characterized in that: In step S5, sodium caseinate and maltooligosaccharide are dissolved in phosphate buffer at a solid-liquid ratio of 1 g:5-10 mL; and the emulsifier is sucrose lipid.

7. The method for preparing a vitamin D-rich Xanthoceras sorbifolia oil powdered fat as claimed in claim 6, characterized in that: In step S5, the rotation speed of the high shear mixer is 10000 rpm.

8. The method for preparing a vitamin D-rich Xanthoceras sorbifolia oil powder as claimed in claim 7, characterized in that: In step S6, the inlet temperature of the spray dryer is 150°C-160°C, and the outlet temperature of the spray dryer is 60°C.

9. The method for preparing a vitamin D-rich Xanthoceras sorbifolia oil powdered fat as claimed in claim 8, characterized in that: In step S6, the flow rate of the emulsion into the spray dryer is 1.8 m³ / min.

10. A powdered Xanthoceras sorbifolia oil rich in vitamin D, wherein the powdered Xanthoceras sorbifolia oil is prepared according to the preparation method according to any one of claims 1 to 9.

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