Chicken intestinal juice enzymolysis fermentation product, preparation method and application thereof, and organic fertilizer

Abstract

The invention relates to a chicken intestine liquid enzymolysis fermentation product, a preparation method and application thereof and an organic fertilizer, and relates to the technical field of organic fertilizers, the preparation method comprises the following steps: performing high-energy electron beam irradiation treatment on chicken intestine liquid to obtain activated chicken intestine liquid; adding a compound enzyme into the activated chicken intestine liquid for enzymolysis to obtain chicken intestine liquid zymolyte; and adding a microbial agent into the chicken intestinal juice zymolyte for fermentation, then filtering, collecting supernatant, concentrating and drying to obtain the chicken intestinal juice zymolyte. The chicken intestine liquid is subjected to enzymolysis fermentation to be converted into the chicken intestine liquid enzymolysis fermentation product which is stable in character and has rich mellow fragrance and fat fragrance, and then the chicken intestine liquid enzymolysis fermentation product is further matched with a proper amount of decomposed organic fertilizer and traditional Chinese medicine extract for use, so that the special organic fertilizer capable of remarkably improving the yield of angelica sinensis is prepared. The innovative achievement not only has remarkable economic benefits, but also provides powerful support for high quality and high yield of the traditional Chinese medicinal materials.

Description

Technical Field

[0001] The invention relates to the technical field of organic fertilizers, and in particular to a chicken intestinal fluid enzymatic fermentation product, a preparation method and application thereof, and an organic fertilizer. Background Art

[0002] In the field of organic fertilizer technology, the search for efficient, environmentally friendly fertilizers that can improve crop yields and quality has always been a hot topic of research. Although traditional organic fertilizers are rich in a variety of nutrients, they often have problems such as slow fertilizer release and lack of specificity, making it difficult to meet the nutrient needs of specific crops. In particular, for some medicinal crops, such as angelica, their nutrient needs during growth are more special, and traditional organic fertilizers are difficult to meet their high-yield and high-quality requirements.

[0003] On the other hand, as an organic waste, chicken intestinal fluid is rich in protein, fat and various trace elements, and has potential fertilizer value. However, there are many inconveniences in the direct application of raw chicken intestinal fluid, such as easy corruption, odor, uneven nutrient release, etc., which limits its wide application in agricultural production.

[0004] Therefore, how to effectively utilize chicken intestinal fluid, an organic waste, and convert it into an efficient, environmentally friendly organic fertilizer that can meet the nutrient needs of specific crops has become an urgent problem to be solved in the current field of organic fertilizer technology. Summary of the invention

[0005] In order to solve the above problems, the present invention provides a chicken intestinal fluid enzymatic fermentation product and a preparation method and application thereof, and an organic fertilizer.

[0006] In a first aspect, the present invention provides a method for preparing chicken intestinal fluid enzymatic fermentation product, the preparation method comprising the following steps:

[0007] The chicken intestinal fluid is irradiated with high-energy electron beams to obtain activated chicken intestinal fluid;

[0008] Adding a composite enzyme to the activated chicken intestinal fluid for enzymolysis to obtain chicken intestinal fluid enzymolysate;

[0009] adding a microbial agent to the chicken intestinal fluid enzymolysis product for fermentation, filtering, collecting the supernatant, concentrating and drying, and obtaining the chicken intestinal fluid enzymolysis fermentation product;

[0010] Wherein, the complex enzyme comprises alkaline protease, enteroprotease and lipase in a weight ratio of (1-5):(1-3):(1-2);

[0011] The microbial agent comprises Bacillus Velez, Bacillus subtilis, yeast and Lactobacillus plantarum in a volume ratio of (1-3):(1-2):(1-2):(1-2), and the abundance of Bacillus Velez, Bacillus subtilis, yeast and Lactobacillus plantarum is 2.0×10 8 ~3.0×10 8 cfu / mL.

[0012] Furthermore, the working condition parameters of the high-energy electron beam irradiation treatment include: the irradiation amount of the high-energy electron beam irradiation treatment is 3 to 8 KGy per 1 g of the chicken intestinal fluid.

[0013] Furthermore, the working condition parameters of the enzymatic hydrolysis include: pH of 8.0-8.5, temperature of 50-60°C, time of 3-5h, and the added weight of the complex enzyme is 0.01-0.05g per 1g of the activated chicken intestinal fluid.

[0014] Furthermore, the working condition parameters of the fermentation include: a temperature of 30 to 40° C., a time of 25 to 35 hours, and an added weight of the microbial agent of 0.01 to 0.05 g per 1 g of the activated chicken intestinal fluid.

[0015] Furthermore, the fermentation broth is filtered using a filter membrane with a molecular weight cutoff of 1500 to 1800 Da.

[0016] In a second aspect, the present invention provides a chicken intestinal fluid enzymatic hydrolysis and fermentation product, wherein the chicken intestinal fluid enzymatic hydrolysis and fermentation product is prepared by the preparation method of the chicken intestinal fluid enzymatic hydrolysis and fermentation product according to any one of the first aspects.

[0017] In a third aspect, the present invention provides a use of the chicken intestinal fluid enzymatic hydrolysis and fermentation product described in any one of the second aspects in the preparation of organic fertilizer.

[0018] In a fourth aspect, the present invention provides an organic fertilizer, comprising the chicken intestinal fluid enzymatic fermentation product described in any one of the second aspects.

[0019] Furthermore, the organic fertilizer comprises the following raw materials in parts by weight:

[0020] 30-50 parts of the chicken intestinal fluid enzymatic fermentation product according to any one of the second aspects, 80-120 parts of decomposed organic fertilizer and 3-8 parts of Chinese herbal medicine extract;

[0021] The Chinese medicine extract is obtained by adding alligator mouth flower and two-color limonium in a weight ratio of (39-47): (7-15) into water for reflux extraction and concentrating and drying the water extract.

[0022] The above technical solution provided by the embodiment of the present invention has at least the following advantages compared with the prior art:

[0023] The embodiment of the present invention provides a chicken intestinal fluid enzymatic fermentation product and its preparation method and application, and organic fertilizer. The present invention performs enzymatic fermentation on chicken intestinal fluid to convert it into a chicken intestinal fluid enzymatic fermentation product with stable properties and rich mellow and fatty aroma, and then further uses it with an appropriate amount of decomposed organic fertilizer and Chinese medicine extract to prepare a special organic fertilizer that can significantly increase the yield of angelica. This innovative achievement not only has significant economic benefits, but also provides strong support for the high quality and high yield of Chinese medicinal materials. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0026] In a first aspect, the present invention provides a method for preparing chicken intestinal fluid enzymatic fermentation product, the preparation method comprising the following steps:

[0027] The chicken intestinal fluid is irradiated with high-energy electron beams to obtain activated chicken intestinal fluid;

[0028] Adding a composite enzyme to the activated chicken intestinal fluid for enzymolysis to obtain chicken intestinal fluid enzymolysate;

[0029] adding a microbial agent to the chicken intestinal fluid enzymolysis product for fermentation, filtering, collecting the supernatant, concentrating and drying, and obtaining the chicken intestinal fluid enzymolysis fermentation product;

[0030] Wherein, the complex enzyme comprises alkaline protease, enteroprotease and lipase in a weight ratio of (1-5):(1-3):(1-2);

[0031] The microbial agent comprises Bacillus Velez, Bacillus subtilis, yeast and Lactobacillus plantarum in a volume ratio of (1-3):(1-2):(1-2):(1-2), and the abundance of Bacillus Velez, Bacillus subtilis, yeast and Lactobacillus plantarum is 2.0×10 8 ~3.0×10 8 cfu / mL.

[0032] The embodiment of the present invention provides an organic fertilizer, which converts chicken intestinal fluid into a chicken intestinal fluid enzymatic fermentation product with stable properties and rich mellow and fatty aroma, and then further uses it with an appropriate amount of decomposed organic fertilizer and Chinese medicine extract to produce a special organic fertilizer that can significantly increase the yield of angelica sinensis. This innovative achievement not only has significant economic benefits, but also provides strong support for the high quality and high yield of Chinese medicinal materials.

[0033] The chicken intestine liquid in the present invention refers to fresh chicken intestines from slaughterhouses that are broken into 3-5 cm segments, added into water for steaming, solid chicken intestines are separated and concentrated to a liquid with a solid content of 55-65 wt%.

[0034] In the present invention, alkaline protease, enteroproteinase, lipase, Bacillus Velez, Bacillus subtilis, yeast and Lactobacillus plantarum can directly use commercially available products without special restrictions. For example, alkaline protease can directly use the commercially available product of Hebei Lihua Biotechnology Co., Ltd., enteroproteinase can directly use the commercially available product of Shanghai Fusheng Industrial Co., Ltd., lipase can directly use the commercially available product of Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd., Bacillus Velez and Bacillus subtilis can directly use the commercially available product of Shandong Yihao Biotechnology Co., Ltd., yeast can directly use the commercially available product of Angel Yeast Co., Ltd., Lactobacillus plantarum can directly use the commercially available product of Guangzhou Huihe Biotechnology Co., Ltd., etc.

[0035] In some specific embodiments, the working condition parameters of the high-energy electron beam irradiation treatment include: the irradiation amount of the high-energy electron beam irradiation treatment is 3 to 8 KGy per 1 g of the chicken intestinal fluid.

[0036] In some specific embodiments, the working condition parameters of the enzymatic hydrolysis include: pH 8.0-8.5, temperature 50-60°C, time 3-5h, and the added weight of the complex enzyme is 0.01-0.05g per 1g of the activated chicken intestinal fluid.

[0037] In some specific embodiments, the working condition parameters of the fermentation include: a temperature of 30-40° C., a time of 25-35 h, and an added weight of the microbial agent of 0.01-0.05 g per 1 g of the activated chicken intestinal fluid.

[0038] In some specific embodiments, the fermentation broth is filtered using a filter membrane with a molecular weight cutoff of 1500-1800 Da, preferably 1700 Da.

[0039] In a second aspect, based on the same inventive concept, the present invention provides a chicken intestinal fluid enzymatic hydrolysis and fermentation product, wherein the chicken intestinal fluid enzymatic hydrolysis and fermentation product is prepared by the preparation method of the chicken intestinal fluid enzymatic hydrolysis and fermentation product described in any one of the first aspects.

[0040] The chicken intestinal fluid enzymatic hydrolysis and fermentation product provided by the present invention is achieved based on the preparation method of the chicken intestinal fluid enzymatic hydrolysis and fermentation product described in any one of the first aspects, and therefore has at least the beneficial effect of the organic fertilizer described in any one of the first aspects, which will not be repeated here.

[0041] In a third aspect, based on the same inventive concept, the present invention provides an application of the chicken intestinal fluid enzymatic fermentation product described in any one of the second aspects in the preparation of organic fertilizer.

[0042] In a fourth aspect, the present invention provides an organic fertilizer, comprising the chicken intestinal fluid enzymatic fermentation product described in any one of the second aspects.

[0043] In some specific embodiments, the organic fertilizer comprises the following raw materials in parts by weight:

[0044] 30-50 parts of the chicken intestinal fluid enzymatic fermentation product according to any one of the second aspects, 80-120 parts of decomposed organic fertilizer and 3-8 parts of Chinese herbal medicine extract;

[0045] The Chinese medicine extract is obtained by adding alligator mouth flower and two-color limonium in a weight ratio of (39-47): (7-15) into water for reflux extraction and concentrating and drying the water extract.

[0046] In the present invention, the decomposed organic fertilizer can be commercially available decomposed chicken manure, decomposed cow manure, etc., or directly made by the existing preparation process, such as evenly mixing livestock and poultry manure (such as chicken manure, pig manure, cow manure, sheep manure, etc.) into the compost fermented by composite microorganisms (such as lactic acid bacteria, Bacillus, photosynthetic bacteria, yeast, actinomycetes, acetic acid bacteria, etc.), livestock and poultry manure accounts for about 80% of the total weight of the compost and the composite microorganism is 0.5% of the total weight of the compost material, and then piled into a 2-meter-wide and 1-meter-high strip for composting and fermentation, that is, to obtain decomposed organic fertilizer. Specifically, the decomposed organic fertilizer is decomposed cow manure, which is directly used for commercially available decomposed cow manure (water content 30%, organic matter 15%) such as Hebei Xiong'an Xinying Technology Co., Ltd.

[0047] The Clinacanthus nutans (Burm.f.) Lindau in the present invention is a herbaceous plant of the genus Clinacanthus of the family Acanthaceae, and its aliases include: Qianlizhui, soft thorn grass, Handi grass, bamboo yellow, bamboo leaf green, green arrow, twisted sequence flower, etc.

[0048] The Limonium bicolor (Bunge) Kuntze in the present invention is a perennial herbaceous plant of the genus Limonium of the family Plumbaceae, and its aliases include scorpion flower cauliflower, flea flower, wild spinach, and artemisia selengensis.

[0049] The preparation method of the organic fertilizer of the present invention comprises: stirring and mixing the components.

[0050] In some specific embodiments, the organic fertilizer comprises the following raw materials in parts by weight:

[0051] 45 parts of the chicken intestinal fluid enzymatic fermentation product according to any one of the second aspects, 100 parts of decomposed organic fertilizer and 7 parts of Chinese herbal medicine extract;

[0052] The Chinese medicine extract is obtained by adding alligator mouth flower and two-color limonium in a weight ratio of 43:12 into water for reflux extraction and concentrating and drying the water extract.

[0053] It should be noted that the organic fertilizer provided in the embodiments of the present invention and the component raw materials involved in the preparation method and application thereof, unless otherwise specified or specified, can be directly commercially available products or homemade using existing public preparation methods; at the same time, the steps and parameters involved, unless otherwise specified or specified, can be carried out according to the preparation process and parameter conditions disclosed in the prior art or directly carried out using existing equipment, and the present invention document will not repeat them one by one.

[0054] The present invention will be further described below in conjunction with specific examples. It should be understood that these examples are intended only to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental methods in the following examples that do not specify specific conditions are usually measured according to national standards. If there is no corresponding national standard, then the conditions recommended by the manufacturer are followed.

[0055] Example 1

[0056] This example provides a chicken intestinal fluid enzymatic hydrolysis fermentation product, and the preparation method of the chicken intestinal fluid enzymatic hydrolysis fermentation product comprises the following steps:

[0057] The chicken intestinal fluid is subjected to high-energy electron beam irradiation treatment, wherein the irradiation amount of the high-energy electron beam irradiation treatment is 5 KGy per 1 g of the chicken intestinal fluid, to obtain activated chicken intestinal fluid;

[0058] Adding a composite enzyme to the activated chicken intestinal fluid for enzymolysis to obtain chicken intestinal fluid enzymolysate;

[0059] Adding a microbial agent to the chicken intestinal fluid enzymolysis product for fermentation, filtering the fermentation liquid with a filter membrane with a molecular weight cutoff of 1700Da, collecting the supernatant and concentrating and drying it to obtain the chicken intestinal fluid enzymolysis fermentation product;

[0060] The composite enzyme includes alkaline protease, intestinal protease and lipase in a weight ratio of 3:2:1.5; the working condition parameters of the enzymatic hydrolysis include: pH 8.3, temperature 55°C, time 4h, and the added weight of the composite enzyme is 0.03g per 1g of the activated chicken intestinal fluid;

[0061] The microbial agent comprises Bacillus Velez, Bacillus subtilis, yeast and Lactobacillus plantarum in a volume ratio of 2:1:1:1.5, and the abundance of Bacillus Velez, Bacillus subtilis, yeast and Lactobacillus plantarum is 2.5×10 8 cfu / mL; the working condition parameters of the fermentation include: temperature of 35°C, time of 30h, and the added weight of the microbial agent is 0.02g per 1g of the activated chicken intestinal fluid.

[0062] Example 2

[0063] This example provides a chicken intestinal fluid enzymatic hydrolysis fermentation product, and the preparation method of the chicken intestinal fluid enzymatic hydrolysis fermentation product comprises the following steps:

[0064] The chicken intestinal fluid is subjected to high-energy electron beam irradiation treatment, wherein the irradiation amount of the high-energy electron beam irradiation treatment is 3 KGy per 1 g of the chicken intestinal fluid, to obtain activated chicken intestinal fluid;

[0065] Adding a composite enzyme to the activated chicken intestinal fluid for enzymolysis to obtain chicken intestinal fluid enzymolysate;

[0066] Adding a microbial agent to the chicken intestinal fluid enzymolysis product for fermentation, filtering the fermentation liquid with a filter membrane with a molecular weight cutoff of 1500Da, collecting the supernatant and concentrating and drying it to obtain the chicken intestinal fluid enzymolysis fermentation product;

[0067] The composite enzyme comprises alkaline protease, intestinal protease and lipase in a weight ratio of 1:1:1; the working condition parameters of the enzymatic hydrolysis include: pH 8.0, temperature 50°C, time 5h, and the added weight of the composite enzyme is 0.03g per 1g of the activated chicken intestinal fluid;

[0068] The microbial agent comprises Bacillus Velez, Bacillus subtilis, yeast and Lactobacillus plantarum in a volume ratio of 3:2:2:1, and the abundance of Bacillus Velez, Bacillus subtilis, yeast and Lactobacillus plantarum is 2.5×10 8 cfu / mL; the working condition parameters of the fermentation include: temperature of 40°C, time of 25h, and the added weight of the microbial agent is 0.02g per 1g of the activated chicken intestinal fluid.

[0069] Example 3

[0070] This example provides a chicken intestinal fluid enzymatic hydrolysis fermentation product, and the preparation method of the chicken intestinal fluid enzymatic hydrolysis fermentation product comprises the following steps:

[0071] The chicken intestinal fluid is subjected to high-energy electron beam irradiation treatment, wherein the irradiation amount of the high-energy electron beam irradiation treatment is 8 KGy per 1 g of the chicken intestinal fluid, to obtain activated chicken intestinal fluid;

[0072] Adding a composite enzyme to the activated chicken intestinal fluid for enzymolysis to obtain chicken intestinal fluid enzymolysate;

[0073] adding a microbial agent to the chicken intestinal fluid enzymolysis product for fermentation, filtering the fermentation liquid with a filter membrane with a molecular weight cutoff of 1800Da, collecting the supernatant and concentrating and drying it to obtain the chicken intestinal fluid enzymolysis fermentation product;

[0074] The composite enzyme includes alkaline protease, intestinal protease and lipase in a weight ratio of 5:3:2; the working condition parameters of the enzymatic hydrolysis include: pH 8.5, temperature 60°C, time 3h, and the added weight of the composite enzyme is 0.03g per 1g of the activated chicken intestinal fluid;

[0075] The microbial agent comprises Bacillus Velez, Bacillus subtilis, yeast and Lactobacillus plantarum in a volume ratio of 1:1:1:1, and the abundance of Bacillus Velez, Bacillus subtilis, yeast and Lactobacillus plantarum is 2.5×10 8 cfu / mL; the working condition parameters of the fermentation include: temperature of 30°C, time of 35h, and the added weight of the microbial agent is 0.02g per 1g of the activated chicken intestinal fluid.

[0076] Example 4

[0077] This example provides an organic fertilizer, which includes the following raw materials by weight:

[0078] 45 parts of the chicken intestinal fluid enzymatic fermentation product obtained in Example 1, 100 parts of decomposed organic fertilizer (specifically decomposed cow dung) and 7 parts of Chinese herbal medicine extract;

[0079] The Chinese herbal extract is obtained by adding alligator flower and dicolor blood-replenishing grass in a weight ratio of 43:12 to water for reflux extraction and concentrating and drying the water extract; wherein the working condition parameters of the reflux extraction include: temperature of 105°C, time of 1 hour, number of times of 2 times, and the weight ratio of the total weight of the water, the alligator flower and the dicolor blood-replenishing grass is 10:1.

[0080] Example 5

[0081] This example provides an organic fertilizer, which includes the following raw materials by weight:

[0082] 30 parts of the chicken intestinal fluid enzymatic fermentation product obtained in Example 2, 80 parts of decomposed organic fertilizer (specifically decomposed cow dung) and 3 parts of Chinese herbal medicine extract;

[0083] The Chinese herbal extract is obtained by adding alligator flower and two-color blood-replenishing grass in a weight ratio of 39:7 to water for reflux extraction and concentrating and drying the water extract; wherein the working condition parameters of the reflux extraction include: temperature of 105°C, time of 1 hour, number of times of 2 times, and the weight ratio of the total weight of the water, the alligator flower and the two-color blood-replenishing grass is 10:1.

[0084] Example 6

[0085] This example provides an organic fertilizer, which includes the following raw materials by weight:

[0086] 50 parts of the chicken intestinal fluid enzymatic fermentation product obtained in Example 3, 120 parts of decomposed organic fertilizer (specifically decomposed cow dung) and 8 parts of Chinese medicine extract;

[0087] The Chinese herbal extract is obtained by adding alligator flower and dicolor limonium in a weight ratio of 47:15 to water for reflux extraction and concentrating and drying the water extract; wherein the working condition parameters of the reflux extraction include: temperature of 105°C, time of 1 hour, number of times of 2 times, and the weight ratio of the total weight of the water, the alligator flower and the dicolor limonium is 10:1.

[0088] Comparative Example 1

[0089] This example provides a chicken intestinal fluid enzymatic fermentation product and a preparation method thereof, which is different from Example 1 only in that the chicken intestinal fluid is not irradiated with a high-energy electron beam; the remaining steps and parameters are the same.

[0090] Comparative Example 2

[0091] This example provides an organic fertilizer, which is different from Example 4 only in that the chicken intestinal fluid enzymatic fermentation product is obtained by Comparative Example 1; the remaining steps and parameters are the same.

[0092] Comparative Example 3

[0093] This example provides an organic fertilizer, which is different from Example 4 only in that: the Chinese herbal medicine extract is obtained by adding Limonium bicolor and Alligator Mouth Flower in a weight ratio of 43:12 to water for reflux extraction and concentrating and drying the water extract; the remaining steps and parameters are the same.

[0094] Test Case

[0095] This example examines the effect of the organic fertilizers obtained from Examples 4 to 6 and Comparative Examples 2 to 3 as a special base fertilizer for the cultivation of Angelica sinensis (the planting variety is Mingui No. 2), and a commercially available nitrogen-phosphorus-potassium compound fertilizer (15-15-15) is used as a basic control group.

[0096] 1. Experimental materials and instruments:

[0097] Organic fertilizer: the organic fertilizer obtained in Examples 4 to 6 and Comparative Example 2;

[0098] Angelica seeds: Mingui No. 2;

[0099] Ground film: used for covering cultivation;

[0100] High performance liquid chromatography: used to determine the ferulic acid content in Angelica sinensis.

[0101] 2. Experimental method: Each test group only used the organic fertilizer obtained by Examples 4-6 and Comparative Examples 2-3, and the basic control group used commercial nitrogen-phosphorus-potassium compound fertilizer. When preparing the land, 60 kg / mu was applied as a base fertilizer at one time. Except for the different fertilizer application, the growth management methods of each test group were the same, no topdressing was performed, and the mulch film was used for cultivation. The row spacing of angelica seedlings was 0.3m×0.4m, and three groups of repetitions were set for each test group. After the angelica was harvested, the yield of angelica was counted, and the ferulic acid content of angelica was determined by high performance liquid chromatography. The average value of the determination results was taken, and according to the formula: Angelica fresh root yield increase rate (%) = (Angelica fresh root yield of the test group-Angelica fresh root yield of the basic control group) / Angelica fresh root yield of the basic control group, and the test results are shown in Table 1.

[0102] Table 1

[0103] Test Group Increase rate of fresh root yield of Angelica sinensis (%) Example 4 group 31％ Example 5 group 22％ Example 6 group 24％ Application of comparative example 2 13％ Application of comparative example 3 10％

[0104] As can be seen from Table 1, the organic fertilizers provided in Examples 4 to 6 of the present invention can significantly increase the yield of Angelica sinensis, can be used as special fertilizers for Angelica sinensis planting, and have a better promoting effect on Angelica sinensis planting than the organic fertilizers provided in Comparative Examples 2 to 3; in addition, the ferulic acid content of the Angelica sinensis obtained in Example 4 was measured by high performance liquid chromatography, and the ferulic acid content (%) was as high as 0.150, with excellent quality.

[0105] In summary, the embodiments of the present invention provide a chicken intestinal fluid enzymatic fermentation product and its preparation method and application, and organic fertilizer. The present invention performs enzymatic fermentation of chicken intestinal fluid to convert it into a chicken intestinal fluid enzymatic fermentation product with stable properties and rich mellow and fatty aroma, and then further uses it with an appropriate amount of decomposed organic fertilizer and Chinese medicine extract to prepare a special organic fertilizer that can significantly increase the yield of angelica. This innovative achievement not only has significant economic benefits, but also provides strong support for the high quality and high yield of Chinese medicinal materials.

[0106] Various embodiments of the present invention may be presented in the form of a range; it should be understood that the description in the form of a range is only for convenience and brevity, and should not be understood as a rigid limitation on the scope of the present invention; therefore, the range description should be considered to have specifically disclosed all possible sub-ranges and single numerical values ​​within the range. For example, the range description from 1 to 6 should be considered to have specifically disclosed sub-ranges, such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., as well as single numbers within the range, such as 1, 2, 3, 4, 5 and 6, which apply regardless of the range. In addition, whenever a numerical range is indicated herein, it is meant to include any cited number (fractional or integer) within the indicated range.

[0107] The foregoing is merely a specific embodiment of the present invention, which enables those skilled in the art to understand or implement the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features claimed herein.

Claims

1. A method for preparing chicken intestinal fluid enzymatic hydrolysis and fermentation product, characterized in that: The preparation method comprises the following steps: The chicken intestinal fluid is irradiated with high-energy electron beams to obtain activated chicken intestinal fluid; Adding a composite enzyme to the activated chicken intestinal fluid for enzymolysis to obtain chicken intestinal fluid enzymolysate; adding a microbial agent to the chicken intestinal fluid enzymolysis product for fermentation, filtering, collecting the supernatant, concentrating and drying, and obtaining the chicken intestinal fluid enzymolysis fermentation product; Wherein, the complex enzyme comprises alkaline protease, enteroprotease and lipase in a weight ratio of (1-5):(1-3):(1-2); The microbial agent comprises Bacillus Velez, Bacillus subtilis, yeast and Lactobacillus plantarum in a volume ratio of (1-3):(1-2):(1-2):(1-2), and the abundance of Bacillus Velez, Bacillus subtilis, yeast and Lactobacillus plantarum is 2.0×10 8 ~3.0×10 8 cfu / mL.

2. The method for preparing chicken intestinal fluid enzymatic hydrolysis and fermentation product according to claim 1, characterized in that: The working condition parameters of the high-energy electron beam irradiation treatment include: the irradiation amount of the high-energy electron beam irradiation treatment is 3 to 8 KGy per 1 g of the chicken intestinal fluid.

3. The method for preparing chicken intestinal fluid enzymatic hydrolysis and fermentation product according to claim 1, characterized in that: The working condition parameters of the enzymatic hydrolysis include: pH of 8.0-8.5, temperature of 50-60° C., time of 3-5 hours, and the added weight of the complex enzyme is 0.01-0.05 g per 1 g of the activated chicken intestinal fluid.

4. The method for preparing chicken intestinal fluid enzymatic hydrolysis and fermentation product according to claim 1, characterized in that: The working condition parameters of the fermentation include: a temperature of 30 to 40° C., a time of 25 to 35 hours, and an added weight of 0.01 to 0.05 g of the microbial agent per 1 g of the activated chicken intestinal fluid.

5. The method for preparing chicken intestinal fluid enzymatic hydrolysis and fermentation product according to claim 1, characterized in that: The fermentation broth is filtered using a filter membrane with a molecular weight cutoff of 1500 to 1800 Da.

6. A chicken intestinal fluid enzymatic fermentation product, characterized in that: The chicken intestinal fluid enzymatic hydrolysis and fermentation product is prepared by the method for preparing the chicken intestinal fluid enzymatic hydrolysis and fermentation product according to any one of claims 1 to 5.

7. Use of the chicken intestinal fluid enzymatic hydrolysis and fermentation product according to claim 6 in preparing organic fertilizer.

8. An organic fertilizer, characterized in that: The organic fertilizer comprises the chicken intestinal fluid enzymatic fermentation product according to claim 6.

9. The organic fertilizer according to claim 8, characterized in that The organic fertilizer comprises the following raw materials in parts by weight: 30-50 parts of the chicken intestinal fluid enzymatic fermentation product of claim 6, 80-120 parts of decomposed organic fertilizer and 3-8 parts of Chinese herbal medicine extract; The Chinese medicine extract is obtained by adding alligator mouth flower and two-color limonium in a weight ratio of (39-47): (7-15) into water for reflux extraction and concentrating and drying the water extract.