Carvacrol and carvone compounded plant essential oil bactericide and application thereof

Abstract

The invention discloses a carvacrol and carvone compounded plant essential oil bactericide and application thereof. Carvacrol and carvone are compounded to play a synergistic effect, so that the growth and development inhibition effect on botrytis cinerea is greatly enhanced, and the prevention and treatment effect of the essential oil on botrytis cinerea is remarkably improved; meanwhile, the quality guarantee of the tomato fruits is enhanced, the storage period of the tomato fruits can be prolonged, and the sugar degree of the fruits is promoted to be improved. Compared with single use, the compound of the two plant essential oils, namely carvacrol and carvone, has the advantage that the application cost of the essential oil is obviously reduced. Therefore, the compound plant essential oil bactericide disclosed by the invention has a wide application prospect.

Description

Technical Field

[0001] The invention belongs to the field of plant disease prevention and control, and specifically relates to a carvacrol and carvone compound plant essential oil fungicide and application thereof. Background Art

[0002] At present, due to the lack of disease-resistant varieties, the prevention and control of gray mold still relies on chemical control. However, due to the strong adaptability and large degree of variation of pathogens, they are prone to resistance to traditional chemical agents, resulting in a weakening of the control effect year by year. Plant essential oils have been developed and applied in large quantities in recent years because of their broad spectrum of control targets, environmental protection, pollution-free, and not easy to induce drug resistance. However, in order to achieve the ideal inhibitory effect, a high concentration of a single plant essential oil is usually required in the control process, which leads to high application costs and difficulty in use, limiting the application of essential oils in agricultural production. At the same time, there are many problems with the combined use of essential oils, such as the compounding effect is not always synergistic, and there may be antagonism or no obvious synergy; the amount of essential oil used increases after compounding; the cost of use of essential oils increases after compounding. Summary of the invention

[0003] In view of this, one of the objects of the present invention is to provide a carvacrol and carvone compound plant essential oil fungicide, wherein the active ingredients of the fungicide include carvacrol and carvone.

[0004] Preferably, the volume ratio of carvacrol to carvone is 3-1:1-9.

[0005] Preferably, the volume ratio of carvacrol to carvone is 1:1.

[0006] The second object of the present invention is to provide the use of the above-mentioned fungicide in inhibiting the growth and development of Botrytis cinerea.

[0007] Preferably, the inhibition of the growth and development of Botrytis cinerea includes any one or more of the following:

[0008] 1) Inhibit the hyphae growth of Botrytis cinerea;

[0009] 2) Inhibit the germination of Botrytis cinerea spores;

[0010] 3) Inhibit the growth of Botrytis cinerea;

[0011] 4) Destroy the cell membrane structure of Botrytis cinerea.

[0012] The third object of the present invention is to provide the use of the above-mentioned fungicide in preventing and controlling plant gray mold.

[0013] Preferably, the plant is tomato.

[0014] A fourth object of the present invention is to provide an application of a fungicide in maintaining or improving the quality of tomato fruits.

[0015] Preferably, the maintaining or improving tomato fruit quality comprises any one or more of the following:

[0016] 1) Reduce the infection rate of Botrytis cinerea on tomato fruits;

[0017] 2) Reduce the weight loss rate of tomato fruit;

[0018] 3) Slow down the rate of decline in tomato fruit firmness;

[0019] 4) Increase the sugar content in tomato fruit.

[0020] The carvacrol and carvone compound essential oil fungicide of the present invention has a synergistic effect of carvacrol and carvone, which significantly enhances the control effect of the essential oil on gray mold fungi, and at the same time enhances the quality assurance of tomato fruits. The compound of the two plant essential oils significantly reduces the application cost of the essential oils when used alone, and has broad application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 The gray mold disease of tomato caused by the gray mold fungus Botrytis cinerea_CQHM1 (PP227469) strain of the present invention infecting tomato fruit;

[0022] Figure 2 The effect of the plant essential oil of the present invention on the germination of Botrytis cinerea spores;

[0023] Figure 3 The effect of the plant essential oil of the present invention on the number of sclerotia produced by Botrytis cinerea and the germination of sclerotia;

[0024] Figure 4 Effect of the compound essential oil of the present invention on the extracellular conductivity and K of Botrytis cinerea + The impact of leakage volume;

[0025] Figure 5 The effect of the compound essential oil of the present invention on the leakage of extracellular soluble protein and reducing sugar of Botrytis cinerea;

[0026] Figure 6 The therapeutic and protective effects of the compound essential oil of the present invention on gray mold of tomato fruit;

[0027] Figure 7 The effect of the compound essential oil of the present invention on pH and weight loss rate of post-harvest tomato fruit (7d);

[0028] Figure 8 The effect of the compound essential oil of the present invention on the firmness and sugar content of post-harvest tomato fruits (7d). DETAILED DESCRIPTION

[0029] The present invention will be described in detail below in conjunction with examples, which are merely illustrative and are not intended to limit the scope of application of the present invention. The present invention is not limited to the following embodiments or examples, and any modifications and variations made without violating the spirit of the present invention should be included within the scope of the present invention. The experimental materials used in the following examples are commercially available unless otherwise specified.

[0030] Experimental Example 1 Screening of the Optimal Ratio of Plant Essential Oil Compounds

[0031] 1.1 Experimental preparation for determination of antibacterial activity of single plant essential oils

[0032] Dimethyl sulfoxide (DMSO) was used to dissolve carvone (99%, purchased from Anhui Jubang Spice Co., Ltd.) and carvacrol (99%, purchased from Anhui Jubang Spice Co., Ltd.) and prepare 1×10 4 μL / L mother solution; 25% pyraclostrobin (purchased from Shenyang Kechuang Chemical Co., Ltd.) was used as a positive control, and dimethyl sulfoxide was used as a solvent to prepare 1×10 4 μL / L stock solution. According to the preliminary experimental results, the five-level concentration gradient of the drug solution as shown in Table 1 was set for subsequent experiments.

[0033] Table 1 Concentration gradient of single essential oils for indoor toxicity test on Botrytis cinerea

[0034]

[0035] 1.2 Determination of the inhibitory activity of a single agent against the mycelial growth of Botrytis cinerea

[0036] In a clean bench, the prepared mother solutions of carvacrol, carvone and 25% pyraclostrobin were diluted with sterile water containing 0.05% Tween 80 to ten times the concentrations shown in Table 1. Subsequently, in a sterile conical flask, the drug solution was pipetted with a pipette and mixed with pre-sterilized, melted potato dextrose agar medium (PDA) at a ratio of 1:9 (v:v) (to obtain the drug with the concentration shown in Table 1), and after rapid and thorough mixing, the mixture was transferred to a culture dish with a diameter of 90 mm. The control group was treated with PDA medium containing DMSO and a sterile aqueous solution of 0.05% Tween 80 without drug solution. Each treatment was repeated three times.

[0037] In advance, Botrytis cinerea (the Botrytis cinerea strain used in the experiment of this institute is the strain of Botrytis cinerea_CQHM1 (PP227469), which was isolated and screened by the laboratory of the inventor's research group. Its storage condition is -80°C, and the storage medium is an aqueous solution containing 25% glycerol; after inoculating tomato fruits, it can infect tomatoes and cause gray mold ( Figure 1, where A is the control, B and C are inoculated with gray mold at different concentrations (Figure 1). Botrytis cinerea is a widely distributed plant pathogenic fungus that can infect a variety of crops, including but not limited to grapes, strawberries, tomatoes and many flowers. It can be screened by conventional methods. ) was activated and cultured in a light incubator at 22°C for 3 days. A 6mm punch was used to prepare a cake of Botrytis cinerea, and a cake of Botrytis cinerea was placed in the center of each medicated culture medium. The culture was carried out in a light incubator at 22°C. When the control colony grew to 2 / 3 of the plate area, the cross method was used to measure the colony diameter, and the mycelium growth inhibition rate was calculated according to formula (1). The DPS software was then used to calculate the toxicity regression equation and the inhibition concentration (EC 50 ).

[0038]

[0039] The results are shown in Table 2. The EC values ​​of carvacrol and carvone 50 The values ​​were 117.23 μL / L and 188.92 μL / L, respectively, which were about twice that of the drug control 25% pyraclostrobin, and the correlation coefficient was reliable and stable.

[0040] Table 2 Results of the antibacterial activity test of a single agent on the growth of Botrytis cinerea hyphae

[0041]

[0042] 1.3 Determination of antibacterial activity of compound essential oils and screening of optimal ratio

[0043] According to the single plant essential oil EC 50 Different compounding ratios were set for essential oil compounding, and five-level concentration gradients of the drug solution were set. The specific compounding ratios and concentration gradients are shown in Table 3. At the same time, a blank control group was added. Each treatment was repeated 3 times. The antibacterial activity of different treatments was detected and the toxicity regression equation was determined according to the method in 1.2 above.

[0044] Table 3 Concentration gradient of carvacrol-carvone in indoor toxicity test on Botrytis cinerea

[0045]

[0046] The Wadley method was then used to calculate the synergistic coefficient (SR) of the compound reagent to evaluate the combined effect of the compound essential oil. The synergistic coefficient calculated according to formula (2) was used to evaluate the synergistic effect of the compound essential oil and screen out the optimal ratio of the essential oil. (When SR < 0.5, it is an antagonistic effect; when 0.5 ≤ SR ≤ 1.5, it is an additive effect; when SR > 1.5, it is a synergistic effect)

[0047]

[0048] Note: Among them, EC50(th) is the theoretical value, EC 50(ob) is the actual value, A and B represent two medicines, a and b are the proportions of each active ingredient in the mixed medicine

[0049] Table 4 Antibacterial activity test results of compound essential oils on the growth of Botrytis cinerea mycelium

[0050]

[0051] The results (Table 4) showed that the toxicity regression equation of carvacrol and carvone in five different volume combinations had a good fit, and the correlation coefficients were all above 0.98. 50 The values ​​ranged from 70.09 μL / L to 113.79 μL / L, and the synergistic coefficients ranged from 1.56 to 2.06. The compound effects all showed synergistic effects. 50 The value was 70.09 μL / L, and the maximum synergistic coefficient was 2.06, indicating that at this ratio, the synergistic effect of carvacrol and carvone was the most significant, and the control effect was the best. According to this ratio, five different concentrations of compound essential oils were set (Table 5) for subsequent experiments.

[0052] Table 5 The concentration gradient of the biological activity of the compound essential oil against Botrytis cinerea under the optimal ratio

[0053]

[0054] Experimental Example 2 Biological Activity Assay

[0055] 2.1 Effects of the tested essential oils on spore germination of Botrytis cinerea

[0056] From the activated Botrytis cinerea, a 6 mm diameter bacterial cake was taken out by a sterile cork borer and then inoculated into a PDA medium and cultured at 22° C. for 7 days. Subsequently, a sterile aqueous solution containing 0.05% Tween 80 was added thereto, all hyphae were collected, and conidia suspension was obtained by filtering through sterile lens paper.

[0057] Potato dextrose water medium (PDB) containing different concentrations of compound essential oil was prepared, and 100 μL of the above spore suspension was added respectively. A control group was set to be added with 0.05% Tween 80 solution alone. When the spore germination rate of the control group reached more than 90%, the spore germination inhibition rate of different treatment groups was calculated according to formula (3).

[0058]

[0059] The results are as follows Figure 2As shown in the data, the compound essential oil carvacrol-carvone (v:v=1:1) has a significant inhibitory effect on the germination of Botrytis cinerea spores. When the concentration is above 70 μL / L, the inhibitory effect of carvacrol-carvone (v:v=1:1) is significantly different compared with that of a single essential oil, and the good inhibitory ability of carvacrol against gray mold is retained.

[0060] 2.2 Effects of the tested essential oils on the sclerotial production of Botrytis cinerea

[0061] Inoculate Botrytis cinerea cakes on different drug-containing plates. After culturing at 22°C for 14 days, collect sclerotia and record the number. Select 30 sclerotia with similar status and culture them in a 22°C incubator. When the germination rate of the control group reaches 95%, calculate the germination rate of sclerotia in each treatment according to formula (4).

[0062]

[0063] The results are as follows Figure 3 (where A is the number of sclerotia produced and B is the germination rate of sclerotia), when carvacrol and carvone are compounded and acted on gray mold, the number of sclerotia produced by Botrytis cinerea can be significantly reduced and the normal development of Botrytis cinerea sclerotia can be inhibited. The compound essential oil carvacrol-carvone (v:v=1:1) has shown obvious advantages at a concentration of 70 μL / L, which is significantly different from the use of a single essential oil and has completely inhibited the production of sclerotia; when the concentration is increased to above 140 μL / L, the germination rate of Botrytis cinerea sclerotia is basically completely inhibited.

[0064] Experimental Example 3 Effect of compound essential oil on cell membrane structure of Botrytis cinerea

[0065] Prepare spore suspension as described above and dilute the spore concentration to 1 × 10 7 conidia / mL. Pipette 100μL of the spore suspension and add it to 100mL of sterilized PDB medium, seal it and place it in a shaker, and culture it at 22℃ and 150rpm for 7 days. Then collect the mycelium by centrifugation at room temperature and 6000rpm for 10min, and rinse it 3 times with PBS (135mM NaCl, 4.7mMKCl, 10mM Na2HPO4, 2mM NaH2PO4) with pH=7.3. Take 2.5g of mycelium and add it to 27.5mL PBS, add compound essential oils to achieve different concentration gradients, place it in a shaker, and culture it at 22℃ and 150r / min for 6h. 0.05% Tween 80PBS containing DMSO was used as the control, and each treatment was repeated 3 times. Finally, centrifuge at 22℃ and 1500rpm for 10min, retain the supernatant, use a conductivity meter to measure the extracellular conductivity, and use the BestLab Sodium potassium ion composite electrode to detect K + .

[0066] Take 1mL of the above supernatant, add 0.75mL of DNS reagent, mix thoroughly, place in boiling water, take out after 5min and place in ice cubes to cool quickly. Take 200μL of sample from each treatment, place in a 96-well plate, measure the absorbance at 540nm on the microplate reader, and calculate the reducing sugar content. Prepare mycelium samples in the same way as above, and extract Botrytis cinerea RNA according to the instructions of the EASYspin Plant RNA Rapid Extraction Kit. Keep the supernatant and follow the instructions of the microplate detection method in the BCA Protein Quantification Kit: take 25μL of the supernatant from each treatment and add it to the microplate, then add 200μL BCA working solution to each well, shake for 30s, and incubate at 37℃ for 120min. Cool at room temperature, measure the absorbance at 595nm on the microplate reader, and calculate the protein concentration of the sample.

[0067] The results are as follows Figure 4 As shown in A (extracellular conductivity), the compound essential oil of carvacrol-carvone (v:v=1:1) has a great effect on the extracellular conductivity of Botrytis cinerea. With the increase of the concentration of the compound essential oil, the extracellular conductivity of Botrytis cinerea shows an upward trend. Among them, when the concentration of the compound essential oil is 35μL / L, the extracellular conductivity is 1.39 times that of the control group (that is, the concentration of the compound essential oil is 0μL / L, the same below); when the concentration increases to 280μL / L, the extracellular conductivity of Botrytis cinerea is 652μs / cm, which is 2.06 times that of the control group. + The leakage results are as follows Figure 4 B(K + Leakage volume) as shown, Botrytis cinerea K + The leakage amount increased significantly with the increase of the concentration of compound plant essential oil. When the concentration of compound essential oil carvacrol-carvone (v:v=1:1) was 17.5μL / L, the K + The concentration was 1.12 times that of the control group; when the concentration reached 280 μL / L, K + The leakage reaches the maximum value, K + The concentration was 3.72 times that of the control group.

[0068] The results of the effects of compound essential oils on the extracellular soluble protein and extracellular reducing sugar leakage of Botrytis cinerea are as follows: Figure 5(where A is the leakage of extracellular soluble protein, and B is the leakage of extracellular reducing sugar). As shown in the data, the extracellular soluble protein content of Botrytis cinerea showed an upward trend with the increase of essential oil concentration. When the concentration was 17.5 μL / L, the soluble protein content of Botrytis cinerea was 1.28 times that of the control group. At 280 μL / L, the damage to the cell membrane of Botrytis cinerea was the most serious, and the soluble protein content was 4.92 times that of the control group. The extracellular reducing sugar content of Botrytis cinerea showed an increasing trend with the increase of the concentration of the compound essential oil as a whole. At concentrations of 35 μL / L and above, the change trend of the extracellular reducing sugar content of Botrytis cinerea in each treatment group was consistent with that of the positive control pyraclostrobin. At a concentration of 280 μL / L, the extracellular reducing sugar content of Botrytis cinerea was 2.73 times that of the control group.

[0069] In summary, when the plant essential oils carvacrol and carvone were compounded at a ratio of 1:1, the extracellular conductivity and K + All indicators such as leakage, reducing sugar leakage and soluble protein leakage showed significant positive correlation. This shows that the compound plant essential oil can significantly destroy the cell membrane structure of Botrytis cinerea, causing a large amount of intracellular substances to flow out of the cell, and is extremely significantly correlated with each leaked substance. The destruction of the cell membrane of Botrytis cinerea means an imbalance of substances inside and outside the cell, which will directly affect its survival and reproduction ability. This result further confirms that the compound essential oil carvacrol-carvone (v:v=1:1) has a significant inhibitory effect on Botrytis cinerea.

[0070] Experimental Example 4 Effect of compound essential oil on gray mold of postharvest tomato fruit

[0071] Select healthy, uniform-sized, intact-skinned, and similarly mature tomato fruits. Rinse the impurities on the fruit skin with clean water, let the surface moisture dry naturally, place the fruit in a sodium hypochlorite solution (1%, v / v) for 5 minutes, and then rinse the fruit skin with sterile water to remove the residual treatment solution. Finally, wipe dry with sterile filter paper to obtain sterilized tomato fruits.

[0072] Different concentrations of compound essential oils were prepared, and 50 μL of spore suspension was injected into tomatoes using a sterile syringe. After being sealed and stored in a storage room for 24 hours, different concentrations of compound essential oils were sprayed on tomatoes, and then sealed and stored in a storage room at 22°C for one week. The diameter of the lesions was measured by the cross method to determine the inhibition rate of gray mold and evaluate the therapeutic effect of essential oils on tomatoes. The solvent without compound essential oil was used as the blank control, and pyraclostrobin was used as the drug control. Each treatment was repeated 3 times.

[0073] Different concentrations of compound essential oils were prepared and sprayed on tomatoes. After sealing, they were placed in a storage room at 22°C for 24 hours. Subsequently, 50 μL of spore suspension was injected into the middle of the equator of the tomato fruit with a sterile syringe (inner diameter 0.25 mm, needle length 8 mm), immediately sealed, and placed in a storage room at 22°C for one week. The diameter of the lesions was measured by the cross method to determine the inhibition rate of gray mold and evaluate the protective effect of essential oils on tomatoes. The solvent without compound essential oil was used as the blank control, and pyraclostrobin was used as the drug control. Each treatment was repeated 3 times.

[0074] The therapeutic effect of compound essential oil on tomato gray mold Figure 6 As shown in A, at concentrations of 17.5 μL / L and above, the expansion of the diameter of tomato gray mold lesions can be significantly inhibited. At 17.5 μL / L, the inhibition rate of the compound essential oil on tomato gray mold was 34.21%; when the concentration was 280 μL / L, the inhibition rate of the compound essential oil on gray mold reached 100%, which was equivalent to the effect of the control agent pyraclostrobin; the protective effect of the compound essential oil on tomato gray mold is shown in Figure 6 As shown in Figure B, when the concentration reached 17.5 μL / L and above, the lesion area of ​​tomato gray mold was significantly reduced, and the corresponding inhibition rate was 9.34%, and the corresponding control effect was 7.15 times that of the blank control group; at a treatment concentration of 280 μL / L, the corresponding inhibition rate of the compound essential oil was 85.90%, and the control effect was 65.74 times that of the blank control group. This shows that the treatment of post-harvest tomato fruits with compound essential oils can effectively reduce the infection rate of Botrytis cinerea; at the same time, at a concentration of 140 μL / L, compared with a single essential oil, the use of compound essential oil carvacrol-carvone (v:v=1:1) can significantly enhance the therapeutic effect and protective effect on tomato gray mold.

[0075] Tomato hardness test: Use a sterile scalpel to make a cut about 1 cm at the equator of the tomato fruit. 3 The incision was made, and then the hardness meter pointer, which had been adjusted to zero, was uniformly applied to completely penetrate the tomato fruit, and the hardness meter pointer reading at this time was recorded. The hardness was measured before (day 0) and after (day 7) treatment with the test essential oil. Three fruits were measured in each group each time, and each fruit was measured three times at different positions and the data were recorded. The hardness change value was calculated according to formula (5).

[0076] Firmness change (N) = fruit firmness measurement value on day 0 - fruit firmness measurement value on day 7 (5)

[0077] Measurement of tomato weight loss rate: The weight was measured twice before (day 0) and after (day 7) treatment with the test essential oil. Ten tomatoes were randomly selected from each treatment each time, weighed using an electronic balance and the data were recorded. The weight loss rate was calculated according to formula (6).

[0078]

[0079] Determination of pH value: pH was measured before (day 0) and after (day 7) treatment with the test essential oil. Tomatoes were randomly selected each time, 20 g of pulp was cut with a sterile scalpel, ground into juice, and pH was measured using a pH meter. After the reading stabilized, the data was recorded. Each treatment was repeated 3 times, and the pH change was calculated according to formula (7).

[0080] pH change = pH value measured on the 7th day of the fruit - pH value measured on the 0th day of the fruit (7)

[0081] Determination of sugar content: The sugar content was measured before (day 0) and after (day 7) treatment with the test essential oil. 5 g of tomato pulp was taken each time, ground into juice and filtered. The filtrate was added to a high-precision digital sugar meter that had been adjusted to zero using a rubber dropper. The readings were observed and recorded. Each treatment was repeated 3 times, and the sugar content change was calculated according to formula (8).

[0082] Sugar content change = sugar content measured on the 7th day of the fruit - sugar content measured on the 0th day of the fruit (8)

[0083] The results are as follows Figure 7 and 8 As shown, the use of the compound essential oil carvacrol-carvone (v:v=1:1) can effectively reduce the fruit weight loss rate by 5.01%-18.96% ( Figure 7 Line graph), can significantly increase the sugar content of fruit by 2%-4.2% ( Figure 8 In summary, the use of carvacrol-carvone (v:v=1:1) can effectively reduce the weight loss rate of tomato fruit during storage, slow down the attenuation rate of tomato fruit hardness, and increase the sugar content in tomato fruit. This effect is enhanced with the increase of the concentration of plant essential oils. This shows that the use of compound essential oils can significantly extend the shelf life of tomatoes, extend the storage period of tomato fruits, and maintain their high quality during storage.

[0084] Experimental Example 5: Cost Calculation Method for Compound Essential Oils

[0085] Based on the price of plant essential oils, the price of additives (dimethyl sulfoxide, 27.5 yuan / L) and the water consumption (30 L / mu), formula (9) is used to calculate the price of compound essential oils, and formula (10) is used to evaluate the overall application cost changes of compound essential oils.

[0086] Unit price of compound essential oil (yuan) = unit price (B) × b + unit price (C) × c (9) Application cost (yuan / mu) = EC 50 (A) × water consumption × unit price (A) × field application correction factor (10)

[0087] Note: Among them, EC 50 (A) is the inhibitory median concentration of the test agent, unit price (A) is the total price of the test agent and adjuvant, and the field application correction factor is the average recommended dosage (80 mL / mu) of 25% pyraclostrobin (positive control) and its indoor inhibitory median concentration EC 50 The ratio of the value (63.99 μL / L) to the value (63.99 μL / L) is 41.67. Unit price (B) and unit price (C) respectively represent the purchase unit prices of the two active ingredients in the compound essential oil, and b and c respectively represent the ratios of the two active ingredients in the compound essential oil.

[0088] Table 6 Application cost of compound essential oil

[0089]

[0090]

[0091] According to Table 6, compared with the control agent 25% pyraclostrobin, the application costs of carvacrol and carvone were 127.05% and 214.27% higher, respectively, which significantly increased the application cost of the agent. However, after the two essential oils were compounded, the application cost of the essential oils was greatly reduced. The application cost of carvacrol-carvone (v:v=1:1) was reduced by 77.73% compared with the control agent, and by 38.82% and 63.72% respectively compared with the use of these two plant essential oils alone.

[0092] The results of the effects of the compound essential oils on the biological activity of Botrytis cinerea, the cell membrane structure of Botrytis cinerea, the effect on gray mold of post-harvest tomato fruit, and the changes in the application costs of the compound essential oils were summarized. The compound essential oil carvacrol-carvone (v:v=1:1) has good inhibitory effects on the spore germination and sclerotium production of Botrytis cinerea, and has excellent therapeutic and protective effects on gray mold of post-harvest tomato fruit, and has the best synergy on improving the quality of post-harvest tomato fruit. Compared with 25% pyraclostrobin (positive control) and single essential oils, the application cost has decreased significantly, and it has the potential and advantage to be developed into a green plant essential oil for the prevention and treatment of tomato gray mold.

[0093] The conventional techniques and schemes not described in detail in the above embodiments are well known in the art, so they will not be described in detail here. The above embodiments and / or experimental examples describe the preferred embodiments of the present invention in detail, but the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, the technical scheme of the present invention can be subjected to a variety of simple modifications, and these simple modifications all belong to the protection scope of the present invention.

Claims

1. A carvacrol and carvone compound plant essential oil fungicide, characterized in that: The active ingredients of the fungicide include carvacrol and carvone.

2. The bactericide according to claim 1, characterized in that The volume ratio of carvacrol to carvone is 3-1:1-9.

3. The bactericide according to claim 2, characterized in that The volume ratio of carvacrol to carvone is 1:

1.

4. Use of the fungicide according to any one of claims 1 to 3 in inhibiting the growth and development of Botrytis cinerea.

5. The use according to claim 4, characterized in that The inhibition of the growth and development of Botrytis cinerea includes any one or more of the following: 1) Inhibit the hyphae growth of Botrytis cinerea; 2) Inhibit the germination of Botrytis cinerea spores; 3) Inhibit the growth of Botrytis cinerea; 4) Destroy the cell membrane structure of Botrytis cinerea.

6. Use of the fungicide according to any one of claims 1 to 3 in preventing and controlling plant gray mold.

7. The use according to claim 6, characterized in that The plant is tomato.

8. Use of the fungicide according to any one of claims 1 to 3 in maintaining or improving the quality of tomato fruit.

9. The use according to claim 8, characterized in that Maintaining or improving tomato fruit quality includes any one or more of the following: 1) Reduce the infection rate of Botrytis cinerea on tomato fruits; 2) Reduce the weight loss rate of tomato fruit; 3) Slow down the rate of decline in tomato fruit firmness; 4) Increase the sugar content in tomato fruit.

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