Bactericidal composition containing picolinamide and benziothiazolinone

Abstract

The invention provides a bactericidal composition containing picolinamide and benziothiazolinone, wherein the weight ratio of the picolinamide to the benziothiazolinone is 1: 200 to 200: 1. The bactericidal composition can be prepared into missible oil, a suspending agent, a microemulsion, a suspoemulsion, an emulsion in water, a dispersible oil suspending agent, wettable powder, water dispersible granules, a suspended seed coating and the like by adding agriculturally available auxiliaries and / or carriers. The invention also relates to a method and application of the composition for preventing and treating agricultural diseases.

Description

Technical Field

[0001] The invention belongs to the field of agriculture, and particularly relates to a bactericidal composition containing pyridobacteramide and thiophanate-methyl and an application thereof. Background Art

[0002] Florylpicoxamid is a second-generation pyridineamide fungicide with a novel mechanism of action. It acts on the cytochrome bc1 complex of the mitochondrial respiratory system of pathogens and exerts its fungicidal activity by inhibiting mitochondrial respiration at the binding site of the fungal complex IIIQi ubiquinone (i.e., coenzyme Q). Pyridineamide has a preventive effect and can prevent spores from germinating on the leaves; when used in the early stages of crop infection with fungi, it also has a therapeutic effect. Pyridineamide can prevent the growth of mycelium in fungi and hinder the development of spores, thereby preventing the fungus from further spreading in the crop. Pyridineamide is used in a variety of plants such as cereals, grapes, fruit trees, nut trees, vegetables, rapeseed, and sugar beets to prevent and control plant diseases such as powdery mildew, anthracnose, scab, dollar spot, leaf blight, gray mold, black shank, early blight, and sclerotinia.

[0003] The patent application for the original drug compound of pyridoxamide is WO2016109257, and the patent in China is CN107205405B. The pyridoxamide compound has fungicidal activity and the application date is December 18, 2015.

[0004] It is currently known that pyridobactamide can be mixed with tebuconazole and the like to prevent and control crop diseases, and there is a synergistic effect. For example, CN114668012A discloses a fungicide composition containing pyridobactamide and tebuconazole, and there is a synergistic effect between pyridobactamide and tebuconazole, and the pesticide preparation is used to prevent and control diseases of vegetables, fruit trees, corn, peanuts, and wheat caused by pathogens.

[0005] Thiophanate, also known as 1,2-benzisothiazolin-3-one, is a heterocyclic compound of isothiazoles. It is a new type of high-efficiency, low-toxic, broad-spectrum fungicide, mainly used to prevent and treat various diseases such as downy mildew, black spot, scab, anthrax, black pox, rot, canker, black shank, leaf spot, etc. Thiophanate is a protective fungicide. Its mechanism of action is mainly to combine with anions on the surface of the bacterial cell membrane or with sulfhydryl groups, destroy the structure of the bacterial cell nucleus, destroy the synthesis system of proteins and cell membranes, interfere with the metabolism of bacterial cells, inhibit the reproduction of pathogens, and lead to the death of pathogens. This compound has a good control effect on crops caused by bacteria and fungi, and is very safe for crops.

[0006] No reports on the combination of pyridoxamide and thiophanate-methyl were found in the prior art. SUMMARY OF THE INVENTION

[0008] The invention relates to a fungicide composition containing pyridobactamide and thiamethoxam, which has a synergistic effect in preventing and controlling agricultural diseases.

[0009] The present invention mainly includes the following technical solutions.

[0010] A fungicide composition, characterized in that the active ingredients of the fungicide composition contain pyridoxamide and thiophanate-methyl, and the weight ratio of pyridoxamide to thiophanate-methyl is 1:200 to 200:1.

[0011] According to the fungicide composition of the present invention, it is characterized in that the weight ratio of pyridoxamide to thiophanate-methyl is preferably 1:100 to 100:1.

[0012] According to the fungicidal composition of the present invention, it is characterized in that the weight ratio of pyridoxamide to thiophanate-methyl is more preferably 1:75 to 75:1.

[0013] The fungicidal composition according to the present invention is characterized in that it further comprises an agriculturally acceptable adjuvant and / or carrier.

[0014] The bactericidal composition according to the present invention is characterized in that the bactericidal composition is prepared into one or more of emulsifiable concentrates, suspensions, microemulsions, suspoemulsions, aqueous emulsions, dispersible oil suspensions, wettable powders, water-dispersible granules, and suspended seed coating agents.

[0015] The bactericidal composition according to the present invention is characterized in that the agriculturally acceptable adjuvants and / or carriers include: solvents, dispersants, wetting agents, emulsifiers, stabilizers, thickeners, antifreeze agents, adhesives, disintegrants, defoaming agents, warning colors, film-forming agents, and fillers.

[0016] The bactericidal composition according to the present invention is characterized in that the total weight percentage of active ingredients in the bactericidal composition is 1-80%.

[0017] The method for using the fungicide composition of the present invention to prevent and control agricultural diseases is characterized in that the fungicide composition is contacted with the agricultural plant or its propagation material before or after the occurrence of the agricultural disease.

[0018] The fungicidal composition according to the present invention is used for preventing and controlling agricultural diseases.

[0019] The use according to the present invention is characterized in that the agricultural diseases include powdery mildew, leaf spot, head smut, rust, Curvularia leaf spot, large spot, small spot, black shank, sclerotinia, early blight, black spot, downy mildew, gray mold, anthracnose, phytophthora, blossom end rot, leaf spot, black spot, and brown rot occurring on field crops, vegetables, and fruit trees. DETAILED DESCRIPTION OF THE INVENTION

[0021] The present invention relates to a fungicide composition containing pyridobacteramide and thiamethoxam. It is found in research that pyridobacteramide and thiamethoxam have a synergistic effect in preventing and controlling agricultural diseases, and the application of the synergistic composition can also reduce the development of pathogen resistance, reduce the total application amount of pesticides, and improve the prevention and control effect, which is beneficial to the prevention and control of agricultural diseases.

[0022] On the one hand, the present invention provides a fungicidal composition containing pyridobactamide and thiamethoxam, wherein the weight ratio of pyridobactamide to thiamethoxam in the composition is 1:200 to 200:1, preferably 1:100 to 100:1, more preferably 1:75 to 75:1, and further preferably, for example, 1:50 to 50:1, 1:45 to 45:1, 1:40 to 40:1, 1:35 to 35:1, 1:30 to 30:1, 1:25 to 25:1, 1:20 to 20:1, 1:15 to 15:1, 1:10 to 10:1, 1:5 to 5:1, etc.

[0023] The fungicidal composition of the present invention can be prepared into agriculturally usable formulations by adding agriculturally acceptable adjuvants and / or carriers when used, including but not limited to: emulsifiable concentrates, suspensions, microemulsions, suspoemulsions, water emulsions, dispersible oil suspensions, wettable powders, water dispersible granules, suspended seed coating agents, etc.

[0024] In the preparation process of the formulation described in the present invention, the agriculturally acceptable adjuvants and / or carriers used include, but are not limited to: solvents, dispersants, wetting agents, emulsifiers, stabilizers, thickeners, antifreeze agents, adhesives, disintegrants, defoamers, warning colors, film formers, and fillers.

[0025] The solvent includes but is not limited to one or more of dimethylformamide, dimethyl sulfoxide, ethanol, acetone, xylene, dichloromethane, ethyl acetate, cyclohexane, cyclohexanone, N-methylpyrrolidone, coconut fatty acid methyl ester, cyclohexanol, methyl glycol ether, and acetonitrile.

[0026] The dispersant includes, but is not limited to, one or more of lignin sulfonate, nonylphenol polyoxyethylene ether, polyoxyethylene alkyl aryl ether, polyoxyethylene lanolin alcohol, fatty alcohol polyoxyethylene ether, fatty alcohol polyoxyethylene ether phosphate, alkyl naphthalene sulfonate, naphthalenesulfonic acid formaldehyde condensate sodium salt, sodium p-hydroxyphenyl lignin sulfonate, fatty alcohol polyoxyethylene ether sulfate, alkylphenol polyoxyethylene ether formaldehyde condensate, alkyl dimethyl benzyl ammonium salt, p-methoxy fatty amide benzene sulfonic acid, p-methoxy fatty amide benzene sulfonate, and polyoxyethylene sorbitan fatty acid ester.

[0027] The wetting agent includes, but is not limited to, one or more of sodium dodecyl sulfate, calcium dodecylbenzene sulfonate, sodium lauroyl sarcosinate, fatty alcohol polyoxyethylene ether, dioctyl sulfosuccinate, polyoxyethylene alkyl aryl ether phosphate, trisiloxane polyoxyethylene ether, sodium isooctyl succinate sulfonate, sodium oleyl methyl taurate, triphenyl phenol polyoxyethylene ether, castor oil polyoxyethylene ether, polyoxyethylene fatty acid esters, and sorbitan fatty acid esters.

[0028] The emulsifier includes, but is not limited to, one or more of lignin sulfonate, sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, calcium dodecylbenzene sulfonate, naphthalene sulfonate formaldehyde condensate, styrylphenyl polyoxyethylene ether, fatty alcohol polyoxyethylene ether, polyoxyethylene alkyl ether, polyoxyethylene sorbitan fatty acid ester, nonylphenol polyoxyethylene ether phosphate, styrene polyoxyethylene ether ammonium sulfate, sorbitan monostearate, and sorbitan monostearate polyoxyethylene ether.

[0029] The stabilizer includes but is not limited to: one or more of triphenyl phosphite, epoxidized soybean oil, epoxidized linseed oil, dialkyl succinate sulfonate, butyl glycidyl ether, etc.;

[0030] The thickener includes, but is not limited to, one or more of carboxymethyl cellulose, sodium carboxymethyl cellulose, gum arabic, xanthan gum, gelatin, magnesium aluminum silicate, and sodium alginate.

[0031] The antifreeze agent includes, but is not limited to, one or more of ethylene glycol, propylene glycol, glycerol, and polyethylene glycol.

[0032] The binder includes, but is not limited to, one or more of gum arabic, xanthan gum, starch, carboxymethyl cellulose, carboxyethyl cellulose, and gelatin.

[0033] The disintegrant includes, but is not limited to, one or more of sodium bicarbonate, ammonium sulfate, sodium carboxymethyl starch, sodium sulfate, calcium sulfate, and urea.

[0034] The defoaming agent includes, but is not limited to, one or more of butyl phosphate, isobutyl phosphate, n-octanol, and silicone.

[0035] The warning colors include, but are not limited to, one or more of alkaline rhodamine, acid fiery red, acid red B, and acid orange.

[0036] The film-forming agent includes, but is not limited to, one or more of polyvinyl alcohol, gum arabic, carboxymethyl cellulose, and polyethylene glycol.

[0037] The filler / carrier includes solid filler / carrier and liquid filler / carrier, and specific varieties include but are not limited to: kaolin, bentonite, diatomaceous earth, attapulgite, montmorillonite, calcium carbonate, clay; one or more of water, soybean oil, corn oil, castor oil, rapeseed oil, and mineral oil.

[0038] In the bactericidal composition of the present invention, the total weight percentage of the active ingredients is 1-80%, preferably 2-70%, and more preferably 5-60%. For example, the total weight percentage of the active ingredients in the bactericidal composition is as low as 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, etc. The total weight percentage of the active ingredients in the bactericidal composition is as high as 60%, 59%, 58%, 57%, 56%, 55%, 54%, 53%, 52%, 51%, 50%, 49%, 48%, 47%, 46%, 45%.

[0039] Specific:

[0040] In one embodiment of the present invention, the bactericidal composition of the present invention is prepared into an emulsifiable concentrate, and its formula composition is: 5-60% active ingredient, 2-20% emulsifier, 2-10% dispersant, and the solvent is supplemented to 100%. The preparation method is: dissolving each component in a solvent and mixing evenly to obtain an emulsifiable concentrate.

[0041] In one embodiment of the present invention, the bactericidal composition of the present invention is prepared into a suspension, and its formula composition is: active ingredient 5-60%, dispersant 2-10%, wetting agent 2-10%, thickener 0.5-5%, antifreeze 0.5-5%, defoamer 0.5-5%, water is supplemented to 100%. Its preparation method is: the active ingredient and various auxiliary agents are mixed with water, and the suspension is obtained after high-speed shear dispersion and sand milling.

[0042] In one embodiment of the present invention, the bactericidal composition of the present invention is prepared into a microemulsion, and its formula composition is: active ingredient 5-60%, solvent 5-30%, emulsifier 2-20%, dispersant 2-20%, thickener 0.5-5%, defoamer 0.5-5%, antifreeze 0.5-5%, and water is supplemented to 100%. Its preparation method is: dissolving the active ingredient in a solvent to prepare an oil phase, adding other components into water and stirring evenly to prepare an aqueous phase, and then adding the oil phase into the aqueous phase while stirring, stirring evenly, to prepare a microemulsion.

[0043] In one embodiment of the present invention, the bactericidal composition of the present invention is prepared into an aqueous emulsion, and its formula composition is: active ingredient 5-60%, solvent 5-20%, emulsifier 2-10%, dispersant 2-10%, thickener 0.5-5%, defoamer 0.5-5%, antifreeze 0.5-5%, and water is supplemented to 100%. Its preparation method is: dissolving the active ingredient in a solvent to prepare an oil phase, adding other components into water and stirring evenly to prepare an aqueous phase, and then adding the oil phase into the aqueous phase while stirring, stirring evenly, to prepare the aqueous emulsion.

[0044] In one embodiment of the present invention, the bactericidal composition of the present invention is prepared into a dispersible oil suspension, and its formula composition is: active ingredient 5-60%, dispersant 2-10%, wetting agent 2-10%, thickener 0.5-5%, and liquid carrier is supplemented to 100%. Its preparation method is: the active ingredient and various auxiliary agents are mixed with the liquid carrier, and the oil suspension is obtained after high-speed shear dispersion and sand milling.

[0045] In one embodiment of the present invention, the fungicidal composition of the present invention is prepared into a wettable powder, and its formula composition is: 5-60% active ingredient, 2-10% dispersant, 2-10% wetting agent, and solid carrier is supplemented to 100%. The preparation method is: mixing the components, grinding and pulverizing to obtain the wettable powder.

[0046] In one embodiment of the present invention, the bactericidal composition of the present invention is prepared into water-dispersible granules, and its formula composition is: active ingredient 5-60%, dispersant 2-10%, wetting agent 2-10%, disintegrant 0.5-5%, binder 0.5-5%, and solid carrier is supplemented to 100%. The preparation method is: the components are mixed uniformly, crushed and granulated to obtain water-dispersible granules.

[0047] Another aspect of the present invention relates to the use of the fungicidal composition for preventing and controlling agricultural diseases.

[0048] Applicable agricultural plants include but are not limited to:

[0049] Field crops: rice, wheat, corn, soybeans, peanuts, rapeseed, etc.

[0050] Vegetables: cucumber, eggplant, tomato, potato, cabbage, strawberry, etc.

[0051] Fruit trees: apple, peach, grape, pear, etc.

[0052] The agricultural diseases that can be used for prevention and control include: powdery mildew, leaf spot, head smut, rust, curvature leaf spot, large spot, small spot, rice blast, black shank, sclerotinia, early blight, black spot, downy mildew, gray mold, anthracnose, phytophthora, blossom end rot, leaf spot, black spot, brown rot, etc.

[0053] Specifically, agricultural diseases include:

[0054] Rice: Rice blast, sheath blight;

[0055] Wheat: powdery mildew, leaf spot;

[0056] Corn: head smut, rust, Curvularia leaf spot, large leaf spot, small leaf spot;

[0057] Peanut: Leaf spot;

[0058] Rapeseed: black leg disease, sclerotinia disease;

[0059] Cucumber: early blight, black spot, downy mildew;

[0060] Tomato: Botrytis cinerea, anthracnose, leaf spot, early blight;

[0061] Pepper: anthracnose, phytophthora;

[0062] Potato: Blackleg;

[0063] Strawberry: Botrytis cinerea;

[0064] Fruit trees: apple blossom end rot, apple leaf spot, pear scab, grape gray mold, peach brown rot.

[0065] Another aspect of the present invention relates to a method for using the fungicidal composition to control agricultural diseases, wherein the fungicidal composition is contacted with the agricultural plant or its propagation material before or after the occurrence of the agricultural disease.

[0066] Specifically, the fungicide composition of the present invention can be applied to the leaves, rhizomes, and seeds of the plants to be treated, especially to the parts that are invaded or easily invaded by pathogens harmful to the growth of crops, by spraying, root irrigation, seed coating, and seed dressing. The application amount and timing can be determined by those skilled in the art, for example, by comprehensive judgment based on the recommended dosage of the agent, the specific occurrence of the disease, and the drug resistance factors of the pathogens. DETAILED DESCRIPTION

[0067] The present invention will be further described below in conjunction with embodiments, but the present invention is not limited thereto.

[0068] The various reagents used in the following examples of the present invention can be obtained from commercial sources or homemade. The plant pathogenic fungi used are obtained by laboratory culture. In addition, unless otherwise specified, the percentages described in the following examples are all weight percentages.

[0069] 1. Combined toxicity test

[0070] 1. Determination of the combined toxicity of pyridoxamide and thiophanate-methyl against Pyricularia oryzae

[0071] Test pathogenic fungi: Rice blast fungus (Pyricularia oryzae);

[0072] Test drugs: pyridobactamide technical drug, thiophanate-methyl technical drug;

[0073] Test method: The toxicity of the agent to pathogenic fungi was determined by the growth rate method, referring to the agricultural industry standard of the People's Republic of China NY / T1156.2006.

[0074] Prepare the agent into a 1000 mg / L mother solution, then take an appropriate amount and mix it according to the predetermined ratio in Table 1 and prepare 5 concentration gradients with an aqueous solution containing 1% Tween-80, then add it to the PDA culture medium at 45-50°C, where the volume ratio of the drug solution to the culture medium is 1:9, mix evenly and pour it into a sterilized culture dish, and form a poisonous culture medium after cooling. Repeat each concentration 3 times. Use an aqueous solution containing 1% Tween-80 as a control. After that, inoculate the pathogen cake into the center of the poisonous culture medium under sterile conditions. Place the culture dish in an incubator with a temperature of 25°C and a humidity of 90% for cultivation. When the colony radius on the control plate grows to about 2 / 3 of the plate radius, measure the colony diameter of each treatment using the cross method, and calculate the inhibition growth rate of each agent treatment on the pathogen by the following formula, and calculate the EC of each treatment on the pathogen 50 .

[0075]

[0076] Then, the EC values ​​of each treatment were measured according to the Sun Yunpei method. 50 Converted into actual toxicity index (ATI); according to the ratio of the mixture, calculated the theoretical toxicity index (TTI), and calculated the co-toxicity coefficient (CTC) of the mixture according to the following formula. The results are shown in Table 1 below.

[0077]

[0078] Theoretical (mixed) toxicity index (TTI) = toxicity index of A × content of A in the mixture (%)

[0079] + Toxicity index of B × content of B in the mixture (%)

[0080]

[0081] If the co-toxicity coefficient is greater than 120, it indicates a synergistic effect; if the co-toxicity coefficient is less than 80, it indicates an antagonistic effect; and if the co-toxicity coefficient is between 100-120, it indicates an additive effect.

[0082] Table 1 Results of indoor toxicity test

[0083] Pharmacy <![CDATA[EC 50 (mg / L)]]> ATI TTI Co-toxicity coefficient CTC (A) Pyridoxamide 0.80 100.00 - - (B) Thiamphenicol 15.21 5.26 - - A:B=200:1 0.89 89.89 99.53 90.31 A:B=100:1 0.71 112.68 99.06 113.74 A:B=75:1 0.64 125.00 98.75 126.58 A:B=50:1 0.66 121.21 98.14 123.51 A:B=25:1 0.63 126.98 96.36 131.79 A:B=5:1 0.67 119.40 84.21 141.79 A:B=1:1 1.17 68.38 52.63 129.92 A:B=1:5 2.89 27.68 21.05 131.51 A:B=1:25 6.05 13.22 8.90 148.52 A:B=1:50 8.33 9.60 7.12 134.94 A:B=1:75 10.16 7.87 6.51 121.02 A:B=1:100 10.84 7.38 6.20 119.08 A:B=1:200 13.12 6.10 5.73 106.40

[0084] The results in Table 1 show that pyridoxamide and thiophanate-methyl have additive or synergistic effects in inhibiting rice blast fungus. For example, in the ratio range of 200:1 to 1:200, they have additive / synergistic effects, especially in the ratio range of 75:1 to 1:75, they all show synergistic effects.

[0085] 2. Determination of the combined toxicity of pyridoxamide and thiophanate-methyl against strawberry gray mold (Botrytis cinerea Pers.)

[0086] A similar method was used to determine the combined toxicity of the composition of the present invention against strawberry gray mold. The results are shown in Table 2 below.

[0087] Table 2 Results of indoor toxicity test

[0088] Pharmacy <![CDATA[EC 50 (mg / L)]]> ATI TTI Co-toxicity coefficient CTC (A) Pyridoxamide 1.51 100.00 - - (B) Thiamphenicol 10.20 14.80 - - A:B=200:1 1.22 123.77 99.58 124.30 A:B=100:1 1.26 119.84 99.16 120.86 A:B=75:1 1.18 127.97 98.88 129.42 A:B=50:1 1.13 133.63 98.33 135.90 A:B=25:1 1.15 131.30 96.72 135.75 A:B=5:1 1.23 122.76 85.80 143.08 A:B=1:1 1.76 85.80 57.40 149.46 A:B=1:5 3.59 42.06 29.00 145.02 A:B=1:25 6.85 22.04 18.08 121.92 A:B=1:50 6.72 22.47 16.47 136.39 A:B=1:75 8.36 18.06 15.92 113.42 A:B=1:100 8.95 16.87 15.65 107.82 A:B=1:200 9.44 16.00 15.23 105.04

[0089] The results in Table 2 show that pyridobacteramide and thiophanate-methyl have additive or synergistic effects in inhibiting strawberry gray mold. For example, in the ratio range of 200:1 to 1:200, they have additive / synergistic effects, especially in the ratio range of 200:1 to 1:50, they all show synergistic effects.

[0090] 3. Determination of the combined toxicity of pyridoxazole and thiophanate-methyl against tomato early blight (Alternaria solani)

[0091] A similar method was used to determine the combined toxicity of the composition of the present invention against tomato early blight pathogen. The results are shown in Table 3 below.

[0092] Table 3 Results of indoor toxicity test

[0093]

[0094]

[0095] The results in Table 3 show that pyridobacteramide and thiophanate-methyl have additive or synergistic effects in inhibiting tomato early blight. For example, within the ratio range of 200:1 to 1:200, they have additive / synergistic effects, especially within the ratio range of 75:1 to 1:5, and even 25:1 to 1:5, they all show synergistic effects.

[0096] 4. Combined toxicity test of pyridamole and thiophanate-methyl against Sclerotinia scleroriorum

[0097] A similar method was used to determine the combined toxicity of the composition of the present invention against Sclerotinia sclerotiorum of cucumber. The results are shown in Table 4 below.

[0098] Table 4 Results of indoor toxicity test

[0099] Pharmacy <![CDATA[EC 50 (mg / L)]]> ATI TTI Co-toxicity coefficient CTC (A) Pyridoxamide 1.47 100.00 - - (B) Thiamphenicol 29.84 4.93 - - A:B=200:1 1.42 103.52 99.53 104.01 A:B=100:1 1.56 94.23 99.06 95.13 A:B=75:1 1.35 108.89 98.75 110.27 A:B=50:1 1.08 136.11 98.14 138.70 A:B=25:1 1.60 91.88 96.34 95.36 A:B=5:1 1.37 107.30 84.15 127.50 A:B=1:1 2.01 73.13 52.46 139.40 A:B=1:5 6.48 22.69 20.77 109.21 A:B=1:25 12.62 11.65 8.58 135.71 A:B=1:50 20.72 7.09 6.79 104.48 A:B=1:75 17.65 8.33 6.18 134.83 A:B=1:100 24.39 6.03 5.87 102.72 A:B=1:200 27.45 5.36 5.40 99.18

[0100] The results in Table 4 show that pyridamole and thiophanate-methyl have additive or synergistic effects in inhibiting cucumber sclerotinia pathogen. For example, within the ratio range of 200:1 to 1:200, they have additive / synergistic effects, among which the ratios with synergistic effects are discretely distributed, specifically 50:1, 5:1, 1:1, 1:25 and 1:75.

[0101] 2. Preparation Examples

[0102] Example 1: Suspension

[0103] Pyridoxamide 20%, thiophanate-methyl 1%, fatty alcohol polyoxyethylene ether 6%, sodium lauryl sulfate 5%, carboxymethyl cellulose 4%, propylene glycol 4%, butyl phosphate 0.5%, and the balance is water.

[0104] The preparation method is as follows: each component is added into water, mixed and stirred, and the suspension is obtained by high-speed shearing, dispersing and grinding.

[0105] Example 2: Suspension

[0106] Pyridoxamide 2%, thiophanate-methyl 10%, sodium lignin sulfonate 3%, trisiloxane polyoxyethylene ether 5%, magnesium aluminum silicate 2%, propylene glycol 4%, butyl phosphate 0.5%, and water as the balance.

[0107] The preparation method is the same as Example 1.

[0108] Example 3 Water emulsion

[0109] Pyridine amide 5%, thiophanate-methyl 5%, dimethylformamide 20%, calcium dodecylbenzenesulfonate 6%, naphthalenesulfonic acid formaldehyde condensate sodium salt 5%, magnesium aluminum silicate 0.6%, n-octanol 0.5%, ethylene glycol 5%, water to 100%.

[0110] The preparation method is as follows: the active ingredient is dissolved in a solvent to prepare an oil phase, the other components are added into water and stirred evenly to prepare an aqueous phase, and then the oil phase is added into the aqueous phase while stirring, and stirred evenly to prepare an aqueous emulsion.

[0111] Example 4 Water emulsion

[0112] Pyridine amide 8%, thiophanate-methyl 0.2%, dimethyl formamide 16%, nonylphenol polyoxyethylene ether phosphate 4%, sodium lignin sulfonate 4%, sodium alginate 0.5%, n-octanol 0.5%, propylene glycol 4%, water makes up to 100%.

[0113] The preparation method is the same as Example 3.

[0114] Example 5 Water Dispersible Granules

[0115] Pyridoxamide 1%, thiophanate-methyl 20%, calcium lignin sulfonate 6%, fatty alcohol polyoxyethylene ether 7%, ammonium sulfate 5%, carboxymethyl cellulose 5%, attapulgite balance.

[0116] The preparation method comprises the following steps: uniformly mixing and crushing the components except the binder, then adding the binder aqueous solution for granulation, and drying to obtain water-dispersible granules.

[0117] Example 6 Water Dispersible Granules

[0118] Pyridoxamide 10%, thiophanate-methyl 2%, calcium lignin sulfonate 4%, trisiloxane polyoxyethylene ether 4%, ammonium sulfate 5%, gelatin 3%, attapulgite balance.

[0119] The preparation method is the same as Example 5.

[0120] Example 7 Wettable powder

[0121] Pyridine amide 0.5%, thiophanate-methyl 20%, nonylphenol polyoxyethylene ether 6%, sodium isooctyl succinate sulfonate 8%, diatomaceous earth is made up to 100%.

[0122] The preparation method is as follows: various components are mixed, ground and crushed to obtain wettable powder.

[0123] Example 8 Wettable powder

[0124] Pyridine amide 15%, thiophanate-methyl 15%, sodium lignin sulfonate 7%, triphenyl phenol polyoxyethylene ether 6%, and bentonite to make up to 100%.

[0125] The preparation method is the same as Example 7.

[0126] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A bactericidal composition, characterized in that: The active ingredients of the fungicide composition contain pyridoxamide and thiamethoxam, and the weight ratio of pyridoxamide to thiamethoxam is 1:200 to 200:

1.

2. The bactericidal composition according to claim 1, characterized in that The weight ratio of pyridobactamide to thiophanate-methyl is preferably 1:100 to 100:

1.

3. The bactericidal composition according to claim 2, characterized in that The weight ratio of pyridobactamide to thiophanate-methyl is more preferably 1:75 to 75:

1.

4. The bactericidal composition according to any one of claims 1 to 3, characterized in that The fungicidal composition further comprises an agriculturally acceptable adjuvant and / or carrier.

5. The bactericidal composition according to claim 4, characterized in that The bactericidal composition is prepared into one or more of emulsifiable concentrates, suspensions, microemulsions, suspoemulsions, aqueous emulsions, dispersible oil suspensions, wettable powders, water-dispersible granules, and suspended seed coating agents.

6. The bactericidal composition according to claim 4 or 5, characterized in that: The agriculturally acceptable adjuvant and / or carrier includes: one or more of solvents, dispersants, wetting agents, emulsifiers, stabilizers, thickeners, antifreeze agents, adhesives, disintegrants, defoamers, warning colors, film formers, and fillers.

7. The bactericidal composition according to any one of claims 4 to 6, characterized in that The total weight percentage of active ingredients in the bactericidal composition is 1-80%.

8. A method for preventing and controlling agricultural diseases using the fungicidal composition according to any one of claims 1 to 7, characterized in that: The fungicidal composition is contacted with the agricultural plant or its propagation material before or after the occurrence of agricultural diseases.

9. Use of the fungicidal composition according to any one of claims 1 to 7 for preventing and controlling agricultural diseases.

10. The use according to claim 9, characterized in that The agricultural diseases include powdery mildew, leaf spot, head smut, rust, curvature leaf spot, large spot, small spot, black shank, sclerotinia, early blight, black spot, downy mildew, gray mold, anthracnose, phytophthora, blossom end rot, leaf spot, black spot, and brown rot occurring on field crops, vegetables, and fruit trees.

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