Application of S-adenosylmethionine salt in prevention and treatment of plant diseases
By soaking seeds of legumes and spraying the aqueous solution of S-adenosine methionine saline on the seeds of legumes and spraying the plants with S-adenosine methionine saline, the problem of gray spot disease prevention and treatment in legumes that has not been reported in the prior art was solved, and the effect of significantly improving the disease resistance of legumes was achieved.
Patent Information
- Application Number
- CN202510165802.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-14
- Publication Date
- 2025-05-13
AI Technical Summary
There has been no reports in the prior art that S-adenosine methionine is used for the prevention and control of soil-borne diseases in crops, especially the lack of effective methods for the prevention and treatment of grey spots in legume plants.
The concentration of S-adenosine methionine aqueous solution is 10-100ppm and sprayed during plant growth to improve the resistance of legume plants to grey spot disease.
It significantly reduces the invasion of grey spot disease on legume plants, especially the prevention and control rate of grey spot disease for soybeans and kidney beans reaches more than 70%, and is harmless to humans and the environment.
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Figure CN119969411A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of application of S-adenosylmethionine salt, and particularly relates to application of S-adenosylmethionine salt in preventing and controlling plant diseases. Background Art
[0002] Soybean is native to my country and is an important food crop. It can be processed into a variety of foods and side dishes. Soybean is one of the main sources of protein food. It is an indispensable staple food and side dish in people's lives. It plays an extremely important role in the supply of edible protein. Soybean is also one of the main oil crops. Soybean oil accounts for about 1 / 6 of the total output of vegetable oil. It is a high-quality edible oil. For many years, it has been the main edible oil in the northern areas, such as Northeast my country and North China. Soybean is an important industrial raw material with a wide range of uses. It can be used as a raw material for paint, ink, glycerin, plastic, bakelite, artificial wool, artificial fiber, photographic film, fatty acid, lecithin, vitamins, and tannic acid protein. Soybean also has certain uses in medicine, health care, and disease prevention and treatment. It can be used as a good food for patients with low starch, such as diabetic patients. Soybean has a good therapeutic effect on preventing fatty liver and liver atrophy and sclerosis.
[0003] Kidney beans are rich in protein, carbohydrates, dietary fiber, vitamins, and minerals. Every 100 grams of kidney beans contains about 7 grams of protein, 20 grams of carbohydrates, and 6 grams of dietary fiber. In addition, kidney beans are rich in vitamin B, vitamin C, and minerals such as iron, potassium, and magnesium. The nutritional content of kidney beans gives them a variety of health benefits. First, kidney beans help lower cholesterol and blood pressure and prevent cardiovascular diseases. Secondly, kidney beans are rich in antioxidants, which help fight free radical damage and delay aging. In addition, kidney beans help control blood sugar levels and prevent diabetes.
[0004] Gray leaf spot is a common disease of legumes, which affects the yield and quality of beans. Generally, the yield of a plot is reduced by 10-15%, and the yield of a severely damaged plot can be reduced by more than 30%. S-adenosylmethionine (SAM) is an active substance discovered by Italian scientists in rat liver. It is widely present in animals, plants and microorganisms. Its molecular weight is 399 and its molecular formula is C15H22N6O5S. Its biosynthesis in the body is completed by the reaction of methionine (Met) and ATP catalyzed by adenosylmethionine synthetase, and only (SS)-SAM has physiological activity. S-adenosylmethionine can improve cell metabolism, participate in the synthesis and metabolism of nucleic acids, proteins and phospholipids in the body, and is an important substance that is indispensable for maintaining normal cell function and normal metabolism. SAM has great application value and broad application prospects in the fields of pharmaceuticals, food, cosmetics, agriculture, etc.
[0005] In the prior art, there are studies on the S-adenosylmethionine synthetase gene (SAMS) from wild soybeans with different degrees of resistance to low temperature, drought and salt treatment ("Cloning and Functional Analysis of Wild Soybean S-adenosylmethionine Synthetase Gene", Fan Jinping, Bai Xi, etc.); there are also studies on the regulatory effects and regulatory mechanisms of S-adenosylmethionine on microbial secondary metabolism. SAM promotes the synthesis of microbial secondary metabolites mainly in the genus Streptomyces, while there are very few studies on other genera ("Progress in Research on the Regulatory Effects and Mechanisms of S-adenosylmethionine on Microbial Secondary Metabolites", Sun Lihui, Zhang Guohai, etc.); due to the instability of S-adenosylmethionine, it is mostly sold in the form of S-adenosylmethionine salts on the market. In the prior art, there are many application fields and research directions for S-adenosylmethionine salts, but there are no reports on the use of S-adenosylmethionine salts for the prevention and control of soil-borne diseases of crops. Summary of the invention
[0006] In view of the deficiencies of the prior art, the present invention provides the use of S-adenosylmethionine salt in preventing and controlling plant diseases.
[0007] When the inventors were studying the effects of S-adenosylmethionine on leguminous crops, they first discovered that S-adenosylmethionine, at a certain concentration, has a growth-promoting effect on leguminous plants and is effective in preventing and controlling gray spot disease of leguminous plants.
[0008] Application of S-adenosylmethionine salt in preventing and controlling plant diseases and / or promoting plant growth.
[0009] Preferably according to the present invention, the plant disease is gray leaf spot.
[0010] Preferably according to the present invention, the S-adenosylmethionine salt comprises adenosylmethionine p-toluenesulfonate sulfate.
[0011] Preferably according to the present invention, the plant is a leguminous plant.
[0012] More preferably, the legumes include soybeans and kidney beans.
[0013] Preferably, the specific method of application comprises the following steps: soaking seeds with an aqueous solution of S-adenosylmethionine salt having a concentration of 10-100 ppm, and then planting the soaked seeds;
[0014] Alternatively, seeds are soaked in an aqueous solution of S-adenosylmethionine salt at a concentration of 10-100 ppm, the soaked seeds are planted, and during the growth of the plants, the seeds are sprayed with an aqueous solution of S-adenosylmethionine salt at a concentration of 10-100 ppm.
[0015] More preferably, the leguminous plant seeds are soaked in an aqueous solution of S-adenosylmethionine salt at a concentration of 60-80 ppm for 10-13 hours, and then the soaked seeds are planted;
[0016] Alternatively, leguminous plant seeds are soaked in an aqueous solution of S-adenosylmethionine salt at a concentration of 60-80 ppm for 10-13 hours, the soaked seeds are planted, and during the growth of the plants, the seeds are sprayed with an aqueous solution of S-adenosylmethionine salt at a concentration of 30-60 ppm.
[0017] More preferably, the leguminous plant seeds are soaked in an aqueous solution of S-adenosylmethionine salt at a concentration of 60 ppm for 12 hours, and then the soaked seeds are planted;
[0018] Alternatively, leguminous plant seeds are soaked in an aqueous solution of S-adenosylmethionine salt at a concentration of 60 ppm for 12 hours, the soaked seeds are planted, and the seeds are sprayed with an aqueous solution of S-adenosylmethionine salt at a concentration of 30 ppm at the three-leaf stage.
[0019] Application of S-adenosylmethionine salt in promoting seed germination of leguminous plants.
[0020] Preferably according to the present invention, the specific method of application comprises the following steps: soaking leguminous plant seeds with an aqueous solution of S-adenosylmethionine salt having a concentration of 10-100 ppm.
[0021] More preferably, the leguminous plant seeds are soaked in an aqueous solution of S-adenosylmethionine salt having a concentration of 60-80 ppm for 10-13 hours.
[0022] More preferably, the leguminous plant seeds are soaked for 12 hours in an aqueous solution of S-adenosylmethionine salt at a concentration of 60 ppm.
[0023] Preferably according to the present invention, the leguminous plants include soybeans and kidney beans.
[0024] Preferably according to the present invention, the S-adenosylmethionine salt comprises adenosylmethionine p-toluenesulfonate sulfate.
[0025] The beneficial effects of the present invention include at least the following:
[0026] 1. The present invention first discovered that S-adenosylmethionine salt has a growth-promoting effect on leguminous plants at a certain concentration, and has an effect on the prevention and treatment of gray spot disease of leguminous plants. S-adenosylmethionine salt can significantly reduce the damage of diseases to leguminous plants, and the prevention and treatment rate of gray spot disease of soybeans and kidney beans reaches more than 70%.
[0027] 2. S-adenosylmethionine salt is harmless to humans and the environment. BRIEF DESCRIPTION OF THE DRAWINGS
[0028] Figure 1 This is a diagram of seed germination after soaking in S-adenosylmethionine salt aqueous solution. DETAILED DESCRIPTION
[0029] The technical solution of the present invention is further described below in conjunction with embodiments, but the protection scope of the present invention is not limited thereto.
[0030] The main active substance of the S-adenosylmethionine salt (adenosylmethionine p-toluenesulfonic acid sulfate CAS: 97540-22-2) used in the present invention is S-adenosylmethionine. Since S-adenosylmethionine is unstable, it is sold in the form of S-adenosylmethionine salt on the market.
[0031] The S-adenosylmethionine salt (adenosylmethionine p-toluenesulfonic acid sulfate CAS: 97540-22-2) used in the present invention was purchased from Shandong Freda Biotechnology Co., Ltd.
[0032] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.
[0033] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.
[0034] Example 1
[0035] Effects of S-adenosylmethionine on the germination of soybean seeds
[0036] Test materials: soybean variety "Zhongdou 63"; 95% S-adenosylmethionine salt.
[0037] (1) Seed soaking treatment: Select seeds that are large, full, undamaged, and free of pests and diseases, prepare S-adenosylmethionine salt with deionized water to make 30 ppm and 60 ppm aqueous solutions, and soak the seeds at 4 liters of aqueous solution per 2 kg of seeds at 25°C for 12 hours. For the control, soak the seeds with deionized water.
[0038] (2) Germination treatment: After soaking, wash the seeds with deionized water and place them evenly in a culture dish covered with gauze. Place the culture dish in a light incubator and culture it under the conditions of 10 hours of light / 14 hours of darkness, 25°C with ventilation and 50-60% humidity. On the 7th day of culture, observe the seeds and take photos for record. Figure 1 .
[0039] The results showed that compared with the control, the seeds soaked in S-adenosylmethionine salt aqueous solution had longer roots, higher stems, and better germination. The germination effect was better when soaked in 60ppm aqueous solution.
[0040] Example 2
[0041] Indoor experiment on reducing soybean gray leaf spot disease by soaking seeds with S-adenosylmethionine salt
[0042] Test materials: soybean variety "Zhongdou 63"; soybean leaves infected with soybean gray spot disease; 95% S-adenosylmethionine salt.
[0043] Test method:
[0044] (1) Seed soaking treatment: Select seeds that are large, full, undamaged, and free of pests and diseases. Prepare an aqueous solution of S-adenosylmethionine salt with deionized water to a certain concentration. Soak the seeds at 4 liters of aqueous solution per 2 kg of seeds at 25°C for 12 hours. For the control, soak the seeds with deionized water.
[0045] (2) Sowing: Sow the soaked seeds into germination boxes filled with mud at a density of 5 seeds per germination box, and press them gently into the mud with tweezers until the seeds are no longer visible.
[0046] (3) Cultivation: The germination box was placed in a light incubator, a leaf infected with soybean gray spot was placed in the middle of the incubator, and the germination box was placed 0.1 m away from the infected leaf. The incubation was carried out under the conditions of 10 hours of light / 14 hours of darkness, 25°C with ventilation, and 50-60% humidity. The emergence rate was counted after 5 days, and the plant height was measured and the gray spot infection status was counted after 25 days. The results are shown in Table 1.
[0047] Table 1 Agronomic traits of seeds infected with diseases after soaking in aqueous solution of S-adenosylmethionine salts at different concentrations
[0048]
[0049]
[0050] The results in Table 1 show that at concentrations of 10ppm, 40ppm, 60ppm, 80ppm, and 100ppm, the soybean "Zhongdou 63" treated with S-adenosylmethionine salt had different degrees of improvement in soybean emergence rate, plant height, and resistance to gray leaf spot compared with the control. This indicates that soaking seeds with S-adenosylmethionine salt solution can improve the resistance of soybeans to gray leaf spot and reduce the disease. As can be seen from Table 1, the effect of soaking seeds with 60ppm S-adenosylmethionine salt solution is relatively better.
[0051] Example 3
[0052] Indoor experiment on reducing gray leaf spot disease of kidney bean by soaking seeds with S-adenosylmethionine salt
[0053] Test materials: kidney bean variety "Jiulibai"; kidney bean leaves infected with gray spot disease; 95% S-adenosylmethionine salt.
[0054] Test method:
[0055] (1) Seed soaking treatment: Select seeds that are large, full, undamaged, and free of pests and diseases, prepare S-adenosylmethionine salt with deionized water to a certain concentration of aqueous solution, and soak the seeds at 4 liters of aqueous solution per 2 kg of seeds at 25°C for 12 hours. The control seeds were soaked in deionized water.
[0056] (2) Sowing: Sow the soaked seeds into germination boxes filled with mud at a density of 5 seeds per germination box, and press them gently into the mud with tweezers until the seeds are no longer visible.
[0057] (3) Cultivation: The germination box was placed in a light incubator, a kidney bean leaf infected with gray spot was placed in the middle of the incubator, and the germination box was placed 0.1 m away from the infected leaf. The incubation was carried out under the conditions of 10 hours of light / 14 hours of darkness, 25°C with ventilation, and 50-60% humidity. The emergence rate was counted after 5 days, and the plant height was measured and the gray spot infection status was counted after 25 days. The results are shown in Table 2.
[0058] Table 2 Agronomic traits of seeds infected with diseases after soaking in aqueous solution of S-adenosylmethionine salts at different concentrations
[0059]
[0060]
[0061] The results in Table 2 show that at concentrations of 10ppm, 40ppm, 60ppm, 80ppm, and 100ppm, the emergence rate, plant height, and resistance to gray leaf spot of kidney beans treated with S-adenosylmethionine salt were improved to varying degrees compared with the control. This indicates that soaking seeds in S-adenosylmethionine salt solution can improve the resistance of kidney beans to gray leaf spot and reduce the disease. As can be seen from Table 2, the effect of soaking seeds in 60ppm S-adenosylmethionine salt solution is relatively better.
[0062] Example 4
[0063] Indoor experiment on reducing the damage of gray leaf spot to soybean plants by S-adenosylmethionine
[0064] Soybean (Zhongdou 63) seeds soaked in 60ppm S-adenosylmethionine salt aqueous solution were planted in a culture room (25℃, 10h / 14h light). When the soybeans grew to the three-leaf stage, branching stage, flowering stage, and pod stage, aqueous solutions with different S-adenosylmethionine salt concentrations (10ppm, 30ppm, 60ppm, 100ppm) were used, and deionized water was used as a control to conduct a spraying test. After spraying, two leaves infected with gray spot disease were placed at a distance of 0.5cm from the soybean plants. On the 5th day, the number of gray spots on the leaves was counted, and the control effect of gray spot disease was calculated. The experiment was repeated 3 times, and the control effect (%) = (control gray spot number - treatment gray spot number) / control gray spot number × 100%.
[0065] Table 3. Effect of S-adenosylmethionine salt aqueous solution concentration on the control of soybean leaf gray spot at different stages
[0066]
[0067] The results in Table 3 show that the control effect of the treated soybeans on gray leaf spot disease was significantly improved compared with the control at the concentrations of 10ppm, 30ppm, 60ppm and 100ppm of the S-adenosylmethionine salt aqueous solution, which indicates that the damage of gray leaf spot disease to soybeans can be reduced by spraying the S-adenosylmethionine salt aqueous solution.
[0068] It can be seen from Table 3 that S-adenosylmethionine salt has a significant effect on improving the prevention and control of gray leaf spot disease during the three-leaf stage, branching stage, flowering stage and podding stage of soybean. The best use period and concentration are during the three-leaf stage of soybean and the use concentration is 30ppm, and the control effect on gray leaf spot disease can reach 71.87%.
[0069] Example 5
[0070] Indoor experiment on reducing the damage of gray leaf spot to kidney bean plants by S-adenosylmethionine
[0071] Kidney bean (nine white) seeds soaked in 60ppm S-adenosylmethionine salt aqueous solution were planted in a culture room (25℃, 10h / 14h light). When the kidney beans grew to the three-leaf stage, branching stage, flowering stage, and pod stage, aqueous solutions with different S-adenosylmethionine salt concentrations (10ppm, 30ppm, 60ppm, 100ppm) were used, and deionized water was used as a control to conduct a spraying test. After spraying, two leaves infected with gray spot disease were placed at a distance of 0.5cm from the kidney bean plants. On the 5th day, the number of gray spots on the leaves was counted, and the control effect of gray spot disease was calculated. The experiment was repeated 3 times, and the control effect (%) = (control gray spot number - treatment gray spot number) / control gray spot number × 100%.
[0072] Table 4. Effect of different concentrations of S-adenosylmethionine salt aqueous solution on the prevention and control of gray spot disease on kidney bean leaves at different stages
[0073]
[0074] The results in Table 4 show that at the concentrations of 10ppm, 30ppm, 60ppm and 100ppm of the S-adenosylmethionine salt aqueous solution, the control effect of the treated kidney beans on gray leaf spot was significantly improved compared with the control. This shows that spraying the S-adenosylmethionine salt aqueous solution can reduce the damage of gray leaf spot to kidney beans.
[0075] It can be seen from Table 4 that S-adenosylmethionine salt has a significant effect on improving the prevention and control of gray spot disease during the three-leaf stage, branching stage, flowering stage and pod-setting stage of kidney beans. The best use period and concentration are during the three-leaf stage of kidney beans and the use concentration is 30ppm, and the control effect on gray spot disease can reach 75.67%.
[0076] The present invention first discovered that S-adenosylmethionine salt has a growth-promoting effect on leguminous plants under a certain concentration, and has an effect on the prevention and control of gray spot disease of leguminous plants. S-adenosylmethionine salt can significantly reduce the damage of diseases to leguminous plants, and the prevention and control rate of gray spot disease of soybeans and kidney beans reaches more than 70%.
Claims
1. Application of S-adenosylmethionine salt in preventing and controlling plant diseases and / or promoting plant growth.
2. The use according to claim 1, characterized in that The plant disease is gray leaf spot; Preferably, the S-adenosylmethionine salt comprises adenosylmethionine p-toluenesulfonate sulfate.
3. The use according to claim 1, characterized in that The plant is a leguminous plant; Preferably, the legumes include soybeans and kidney beans.
4. The use according to claim 1, characterized in that The specific application method comprises the following steps: soaking seeds with an aqueous solution of S-adenosylmethionine salt having a concentration of 10-100 ppm, and then planting the soaked seeds; Alternatively, seeds are soaked in an aqueous solution of S-adenosylmethionine salt at a concentration of 10-100 ppm, the soaked seeds are planted, and during the growth of the plants, the seeds are sprayed with an aqueous solution of S-adenosylmethionine salt at a concentration of 10-100 ppm.
5. The use according to claim 4, characterized in that Using an aqueous solution of S-adenosylmethionine salt at a concentration of 60-80 ppm to soak leguminous plant seeds for 10-13 hours, and then planting the soaked seeds; Alternatively, leguminous plant seeds are soaked in an aqueous solution of S-adenosylmethionine salt at a concentration of 60-80 ppm for 10-13 hours, the soaked seeds are planted, and during the growth of the plants, the seeds are sprayed with an aqueous solution of S-adenosylmethionine salt at a concentration of 30-60 ppm.
6. The use according to claim 5, characterized in that Using an aqueous solution of S-adenosylmethionine salt at a concentration of 60 ppm to soak leguminous plant seeds for 12 hours, and then planting the soaked seeds; Alternatively, leguminous plant seeds are soaked in an aqueous solution of S-adenosylmethionine salt at a concentration of 60 ppm for 12 hours, the soaked seeds are planted, and the seeds are sprayed with an aqueous solution of S-adenosylmethionine salt at a concentration of 30 ppm at the three-leaf stage.
7. Application of S-adenosylmethionine salt in promoting seed germination of leguminous plants.
8. The use according to claim 7, characterized in that The specific application method comprises the following steps: soaking leguminous plant seeds with an S-adenosylmethionine salt aqueous solution having a concentration of 10-100 ppm.
9. The use according to claim 8, characterized in that Soaking leguminous plant seeds for 10-13 hours using an aqueous solution of S-adenosylmethionine salt at a concentration of 60-80 ppm; Preferably, the leguminous plant seeds are soaked for 12 hours in an aqueous solution of S-adenosylmethionine salt at a concentration of 60 ppm.
10. The use according to claim 7, characterized in that The legumes include soybeans and kidney beans; Preferably, the S-adenosylmethionine salt comprises adenosylmethionine p-toluenesulfonate sulfate.