Application of S-adenosylmethionine salt in promoting seed germination and plant growth
By using the S-adenosine methionine solution to seed germination and stem thickening problems in the prior art, the effect of improving seed germination rate and stem thickness is achieved, and the resistance to lodging of the seedlings is enhanced.
Patent Information
- Application Number
- CN202510165804.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-14
- Publication Date
- 2025-05-13
AI Technical Summary
There is a lack of effective methods in the prior art to promote seed germination and plant stem thickening, affecting crop emergence and healthy growth.
Seed seed soaking and plant spraying using S-adenosine solution, combined with seed soaking treatment, promote seed germination and plant stem thickening.
It significantly improves the seed germination rate and the thickness of plant stems, enhances the resistance to lodging of seedlings, has the effect of strengthening seedlings, and is suitable for crop cultivation.
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Figure CN119969412A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to application of S-adenosylmethionine salt in promoting seed germination and plant growth, and belongs to the technical field of plant cultivation of S-adenosylmethionine salt. Background Art
[0002] Vegetables are an indispensable food in people's daily life, and the domestic market has a huge demand for vegetables. With the improvement of people's living standards and the enhancement of health awareness, the requirements for the quality and variety of vegetables are getting higher and higher, which provides a broad market space for the development of the vegetable industry.
[0003] Solanaceae plants contain a variety of vitamins and minerals. For example, tomatoes are rich in vitamin C, carotene and B vitamins, and also contain fruit acid, which can reduce people's cholesterol levels; the organic acids contained in tomatoes have the function of promoting digestion and regulating the stomach; the lycopene contained in tomatoes has an antibacterial effect, etc. Cruciferae plants generally have a short growth cycle and strong adaptability. They are rich in bioactive substances, which can be converted into compounds with anti-cancer activity in the body, helping to reduce the risk of cancer.
[0004] The characteristic active ingredients of Cucurbitaceae plants are tetracyclic trirudinoid cucurbitane saponins, such as cucurbitacin, which have anti-cancer activity, can prevent fatty degeneration of liver cells and inhibit liver fibrosis; some Cucurbitaceae plants also have the effects of clearing away heat and reducing internal heat, relieving summer heat and improving eyesight. In addition, some plants also contain highly active proteins and amino acids, such as trichosanthin, which has the effect of inducing labor, and cucurbitacin, which has the effect of expelling insects.
[0005] Therefore, as they are becoming more and more popular among the public, their demand is also expanding, and factory seedling cultivation has become an important method of seedling cultivation. The quality of seedlings is a key factor in ensuring the normal development of crops. During the seedling cultivation process, the quality of seed germination directly affects the germination rate, and then affects the number of groups. Good seed germination can increase the germination rate, increase the number of groups, and lay the foundation for high crop yields; the stems mainly play a supporting and transporting role in the seedling cultivation process. The thickness of the stems has a significant impact on seedling cultivation. Thick stems can enhance the seedlings' resistance to lodging, and can also efficiently transport water and nutrients to promote the healthy growth of crops. Therefore, promoting seed germination rate and ensuring the healthy growth of seedlings are of great significance to achieving high yields.
[0006] S-adenosylmethionine (SAM), also known as S-adenosyl-L-methionine, S-methionine, and thiomethionine, is an important physiologically active substance widely present in plants, animals, and microorganisms. It participates in dozens of biochemical reactions in the body and plays a vital role in the normal metabolic activities of the body. Due to the instability of S-adenosylmethionine, it is mostly sold in the form of S-adenosylmethionine salts on the market. S-adenosylmethionine salts have great application value and wide application prospects in the fields of health products, pharmaceutical preparations, food, cosmetics, animal husbandry, etc. There is no report on the research of S-adenosylmethionine salts in promoting seed germination and promoting the thickening of plant stems in the prior art. Summary of the invention
[0007] In view of the deficiencies of the prior art, the present invention provides the use of S-adenosylmethionine salt in promoting seed germination and plant growth.
[0008] The inventors first discovered that soaking seeds with S-adenosylmethionine salt can promote seed germination, and spraying crops in combination with soaking can promote plant growth, promote the thickening of plant stems, and have the effect of strengthening seedlings, and can be used for crop cultivation and seedling raising.
[0009] The technical solution of the present invention is as follows:
[0010] The application of S-adenosylmethionine salt in plant cultivation includes any of the following applications:
[0011] (1) Application in promoting plant seed germination;
[0012] (2) Application in promoting plant growth;
[0013] (3) Application in plant seedling cultivation.
[0014] According to the present invention, preferably, the application in promoting plant growth or in raising plant seedlings is the application in promoting the thickening of plant stems.
[0015] Preferably according to the present invention, the S-adenosylmethionine salt comprises adenosylmethionine p-toluenesulfonate sulfate.
[0016] Preferably according to the present invention, in the application, the plant comprises Cucurbitaceae or Solanaceae.
[0017] More preferably, the plant is cucumber or tomato.
[0018] Further preferably, in the application of promoting plant seed germination, S-adenosylmethionine salt solution is used for seed soaking;
[0019] In the application in promoting plant growth or in raising plant seedlings, S-adenosylmethionine salt solution is used for soaking seeds, and S-adenosylmethionine salt solution is used for spraying when the seedlings are young.
[0020] Preferably according to the present invention, in the application, the concentration of S-adenosylmethionine salt is 10-30 mg / L.
[0021] Further preferably, in the use of S-adenosylmethionine salt in promoting tomato seed germination, the concentration of S-adenosylmethionine salt is 20 mg / L.
[0022] Further preferably, in the use of S-adenosylmethionine salt in promoting cucumber seed germination, the use concentration of S-adenosylmethionine salt is 10-20 mg / L.
[0023] More preferably, in the use of S-adenosylmethionine salt in promoting cucumber seed germination, the concentration of S-adenosylmethionine salt is 10 mg / L.
[0024] A method for growing plant seedlings comprises the following steps:
[0025] The seeds are soaked in 10-30 mg / L S-adenosylmethionine salt solution, and then planted and raised as seedlings. When the seedlings are young, they are sprayed with 10-30 mg / L S-adenosylmethionine salt solution.
[0026] Preferably according to the present invention, the S-adenosylmethionine salt comprises adenosylmethionine p-toluenesulfonate sulfate.
[0027] Preferably according to the present invention, in the method, the plant comprises Cucurbitaceae or Solanaceae.
[0028] More preferably, the plant is cucumber or tomato.
[0029] Preferably according to the present invention, a method for raising cucumber seedlings comprises the following steps: soaking the seeds with a 10-20 mg / L S-adenosylmethionine salt solution, then planting the seedlings, and spraying the seedlings with a 10-20 mg / L S-adenosylmethionine salt solution.
[0030] Further preferably, a method for raising cucumber seedlings comprises the following steps: soaking the seeds in a 10 mg / L S-adenosylmethionine salt solution, then planting the seedlings, and spraying the seedlings with a 10 mg / L S-adenosylmethionine salt solution.
[0031] Preferably according to the present invention, a method for raising tomato seedlings comprises the following steps: soaking the seeds in a 20 mg / L S-adenosylmethionine salt solution, then planting the seedlings, and spraying the seedlings with a 20 mg / L S-adenosylmethionine salt solution.
[0032] Preferably according to the present invention, in the above application or the above method, the seed soaking method is to soak the seeds in S-adenosylmethionine salt solution for 20-24 hours and then wash with water.
[0033] Preferably according to the present invention, in the above application or the above method, when the seedling grows to have two leaves and one heart, the whole seedling is sprayed.
[0034] Further preferably, when the seedlings grow to two leaves and one bud, the whole seedlings are sprayed once.
[0035] More preferably, 80-100 mL of S-adenosylmethionine salt solution is sprayed on each plant.
[0036] According to the preferred embodiment of the present invention, the solvent of the S-adenosylmethionine salt solution is water.
[0037] More preferably, the solvent of the S-adenosylmethionine salt solution is deionized water.
[0038] The beneficial effects of the present invention include at least the following:
[0039] The present invention firstly finds that when a certain concentration of S-adenosylmethionine salt solution is used to soak seeds, seed germination can be promoted; when a certain concentration of S-adenosylmethionine salt solution is used to spray the whole plant and combined with a seed soaking treatment, the plant stems can be significantly promoted to be thicker, and the thick stems can enhance the lodging resistance of seedlings, have a seedling strengthening effect, and can be used for plant seedling cultivation. At the same time, S-adenosylmethionine salt will not cause pollution to crops, animals and the environment, and is harmless to the human body, and is a green and environmentally friendly production method. BRIEF DESCRIPTION OF THE DRAWINGS
[0040] Figure 1 This is an experimental diagram showing the effect of different S-adenosylmethionine salt concentrations on cucumber seed germination;
[0041] In the figure: CK is the control group, 10, 20, 30 are the groups with S-adenosylmethionine salt concentration of 10 mg / L, 20 mg / L, and 30 mg / L, respectively.
[0042] Figure 2 This is an experimental diagram showing the effect of spraying the same concentration of S-adenosylmethionine salt solution on tomato seedlings under different soaking concentration conditions.
[0043] Figure 3 This is an experimental diagram showing the effect of spraying the same concentration of S-adenosylmethionine salt solution on cucumber seedlings under different soaking concentration conditions. DETAILED DESCRIPTION
[0044] The present invention is further described below in conjunction with embodiments, but the protection scope of the present invention is not limited thereto.
[0045] The main active substance of the S-adenosylmethionine salt (adenosylmethionine p-toluenesulfonic acid sulfate CAS: 97540-22-2) used in the present invention is S-adenosylmethionine. Since S-adenosylmethionine is unstable, it is sold in the form of S-adenosylmethionine salt on the market.
[0046] The S-adenosylmethionine salt (adenosylmethionine p-toluenesulfonic acid sulfate CAS: 97540-22-2) used in the present invention was purchased from Shandong Freda Biotechnology Co., Ltd.
[0047] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.
[0048] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.
[0049] Example 1
[0050] The experiment of S-adenosylmethionine salt promoting plant seed germination is as follows:
[0051] Select seeds with full grains, soak them in 0.05% NaClO solution for 3 h, and then rinse them with deionized water 4-5 times to remove impurities on the seed surface.
[0052] The pretreated seeds were soaked in 200 mL of deionized water and S-adenosylmethionine salt solutions with final concentrations of 10 mg / L, 20 mg / L, 30 mg / L, 40 mg / L and 50 mg / L for 24 h, respectively.
[0053] The S-adenosylmethionine salt solution is prepared with deionized water.
[0054] The soaked seeds were cleaned with deionized water and evenly placed in a culture dish covered with gauze, with 100 seeds per dish. Cultured in the dark at 28°C, the germination potential of the seeds was counted on the 3rd day of culture, and the germination rate of the seeds was counted on the 7th day of culture. Each treatment group was repeated 3 times. Germination potential = number of seeds germinated within 3 days / total number of seeds * 100%, germination rate = number of seeds germinated at the end of germination / total number of seeds * 100%, the specific results are shown in Table 1:
[0055] Table 1 Effects of different concentrations of S-adenosylmethionine salt solutions on the germination rate of plant seeds
[0056]
[0057] The results in Table 1 show that the plant seeds treated with 10-50 mg / L S-adenosylmethionine salt solution have a significant effect on growth compared with the control group, and the specific manifestations are:
[0058] For Cucurbitaceae plants, 10-20 mg / L S-adenosylmethionine salt solution can improve the germination rate of cucumber seeds. Among them, 10 mg / L S-adenosylmethionine salt solution has the best effect on promoting the germination rate of cucumber seeds ( Figure 1 ). When the concentration of S-adenosylmethionine salt is higher than 20 mg / L, the germination rate of cucumber seeds decreases.
[0059] For Solanaceae plants, the germination rate of tomato seeds treated with 10-30 mg / L S-adenosylmethionine salt solution was significantly higher than that of the control group. Among them, 20 mg / L S-adenosylmethionine salt had the best effect on promoting tomato seed germination, and the germination rate of tomato seeds increased by 14.9% compared with the control group. However, the germination rate of tomato seeds treated with 40 mg / L and 50 mg / L S-adenosylmethionine salt solution decreased compared with the control group. It is possible that the excessively high S-adenosylmethionine salt concentration inhibited the germination of tomato seeds.
[0060] Example 2
[0061] Effects of S-adenosylmethionine on the Growth of Plant Seedlings
[0062] The seeds treated with deionized water, 10 mg / L, 20 mg / L and 30 mg / L S-adenosylmethionine salt solution (seed soaking treatment according to the method in Example 1) were moved to a seedling pot with nutrient soil as the matrix, containing a small amount of perlite and vermiculite, 500 g of nutrient soil per pot, soil moisture of 60%-70%, pH 6-7. The seeds were cultured in a 25°C greenhouse (16 h light / 8 h dark), environmental humidity of 45%-50%, and when the seedlings grew to two leaves and one heart, the whole seedlings (the aboveground part of the seedlings) were sprayed with S-adenosylmethionine salt solution, spraying once, and the spraying volume per plant was 100 mL.
[0063] The experiment was set up with 16 treatments, divided into a control group and an experimental group, as follows:
[0064] Control group:
[0065] CK1 (seed soaking in deionized water and spraying with deionized water), CK2 (seed soaking in deionized water and spraying with 10 mg / L S-adenosylmethionine salt solution), CK3 (seed soaking in deionized water and spraying with 20 mg / L S-adenosylmethionine salt solution), and CK4 (seed soaking in deionized water and spraying with 30 mg / L S-adenosylmethionine salt solution).
[0066] Experimental Group:
[0067] A1 (seed soaking in 10 mg / L S-adenosylmethionine salt solution and spraying with deionized water), A2 (seed soaking in 10 mg / L S-adenosylmethionine salt solution and spraying with 10 mg / L S-adenosylmethionine salt solution), A3 (seed soaking in 10 mg / L S-adenosylmethionine salt solution and spraying with 20 mg / L S-adenosylmethionine salt solution), A4 (seed soaking in 10 mg / L S-adenosylmethionine salt solution and spraying with 30 mg / L S-adenosylmethionine salt solution);
[0068] B1 (seed soaking in 20 mg / L S-adenosylmethionine salt solution and spraying with deionized water), B2 (seed soaking in 20 mg / L S-adenosylmethionine salt solution and spraying with 10 mg / L S-adenosylmethionine salt solution), B3 (seed soaking in 20 mg / L S-adenosylmethionine salt solution and spraying with 20 mg / L S-adenosylmethionine salt solution), B4 (seed soaking in 20 mg / L S-adenosylmethionine salt solution and spraying with 30 mg / L S-adenosylmethionine salt solution);
[0069] C1 (seeds soaked in 30 mg / L S-adenosylmethionine salt solution and sprayed with deionized water), C2 (seeds soaked in 30 mg / L S-adenosylmethionine salt solution and sprayed with 10 mg / L S-adenosylmethionine salt solution), C3 (seeds soaked in 30 mg / L S-adenosylmethionine salt solution and sprayed with 20 mg / L S-adenosylmethionine salt solution), C4 (seeds soaked in 30 mg / L S-adenosylmethionine salt solution and sprayed with 30 mg / L S-adenosylmethionine salt solution).
[0070] Each treatment was repeated three times, with 30 seedlings in each group. The seedlings were cultured for 30 days, and 10 seedlings were randomly selected to measure the plant height and stem diameter of the seedlings in each treatment group. The plant height was tested above the root, and the stem diameter was tested at the cotyledon node.
[0071] Table 2 Effects of different concentrations of S-adenosylmethionine salt solution on the growth of tomato seedlings of Solanaceae
[0072]
[0073] Table 3 Effects of different concentrations of S-adenosylmethionine salt solution on the growth of cucumber seedlings of Cucurbitaceae
[0074]
[0075] The above results show that S-adenosylmethionine can significantly affect the growth of plant seedlings, and the specific manifestations are:
[0076] For the Solanaceae plant tomato (Table 2), the stem thickness of the seedlings cultivated from seeds treated with S-adenosylmethionine salt solution at a concentration of 10-30 mg / L was significantly higher than that of the seedlings cultivated after soaking in deionized water (group CK); among them, soaking the seeds in 20 mg / L S-adenosylmethionine salt solution and spraying 20 mg / L S-adenosylmethionine salt solution significantly increased the stem thickness of the seedlings.
[0077] In the same group, the seed soaking concentration was the same, but the seed spraying concentration was different, and the plant height of the seedlings cultivated had no obvious difference; in different groups, the seed soaking concentration was the same, but the seed spraying concentration was different, and the plant height of the seedlings cultivated had certain differences ( Figure 2 ), indicating that the appropriate concentration of S-adenosylmethionine salt has promoted plant growth during the seed germination period.
[0078] For Cucurbitaceae plant cucumber (Table 3), S-adenosylmethionine salt solution with a concentration of 10-20 mg / L can promote the thickening of the stems of cucumber seedlings (Groups A and B); among them, soaking seeds with 10 mg / L S-adenosylmethionine salt solution and spraying with 10 mg / L S-adenosylmethionine salt solution have the most obvious effect on increasing the thickness of the seedling stems. Seedlings grown from seeds treated with 10-20 mg / L S-adenosylmethionine salt solution are superior to seedlings grown from seeds not treated with S-adenosylmethionine salt solution and seeds treated with 30 mg / L S-adenosylmethionine salt solution (Table 3, Figure 3 ).
[0079] In summary, the present invention finds that soaking seeds in a certain concentration of S-adenosylmethionine salt solution can promote seed germination; spraying the whole plant with a certain concentration of S-adenosylmethionine salt solution and combining with seed soaking treatment can significantly promote the thickening of plant stems, and the thick stems can enhance the lodging resistance of seedlings, have a seedling strengthening effect, and can be used for plant seedling cultivation. At the same time, S-adenosylmethionine salt is non-toxic, does not cause pollution to crops, animals and the environment, meets the requirements for promoting plant growth, and is green and healthy, harmless to humans and the environment.
Claims
1. The application of S-adenosylmethionine salt in plant cultivation includes any of the following applications: (1) Application in promoting plant seed germination; (2) Application in promoting plant growth; (3) Application in plant seedling cultivation.
2. The use according to claim 1, characterized in that Application in promoting plant growth or in plant seedling cultivation, and application in promoting the thickening of plant stems; Preferably, in the application of promoting plant seed germination, S-adenosylmethionine salt solution is used for seed soaking; In the application in promoting plant growth or in raising plant seedlings, S-adenosylmethionine salt solution is used for soaking seeds, and S-adenosylmethionine salt solution is used for spraying when the seedlings are young.
3. The use according to claim 1, characterized in that In the application, the plant includes Cucurbitaceae or Solanaceae; Preferably, the plant is cucumber or tomato; Preferably, the S-adenosylmethionine salt comprises adenosylmethionine p-toluenesulfonate sulfate.
4. The use according to claim 1, characterized in that The concentration of S-adenosylmethionine salt used is 10-30 mg / L.
5. The use according to claim 4, characterized in that The application of S-adenosylmethionine salt in promoting tomato seed germination, the concentration of S-adenosylmethionine salt is 20mg / L; Preferably, the use of S-adenosylmethionine salt in promoting cucumber seed germination, the use concentration of S-adenosylmethionine salt is 10-20 mg / L; Preferably, in the use of S-adenosylmethionine salt in promoting cucumber seed germination, the concentration of S-adenosylmethionine salt is 10 mg / L.
6. A method for growing plant seedlings, characterized in that: The steps include: The seeds are soaked in 10-30 mg / L S-adenosylmethionine salt solution, and then planted and raised as seedlings. When the seedlings are young, they are sprayed with 10-30 mg / L S-adenosylmethionine salt solution.
7. The method according to claim 6, characterized in that In the method, the plant comprises Cucurbitaceae or Solanaceae; Preferably, the plant is cucumber or tomato; Preferably, the S-adenosylmethionine salt comprises adenosylmethionine p-toluenesulfonate sulfate.
8. The method according to claim 7, characterized in that A method for raising cucumber seedlings comprises the following steps: soaking seeds with a 10-20 mg / L S-adenosylmethionine salt solution, then planting and raising seedlings, and spraying the seedlings with a 10-20 mg / L S-adenosylmethionine salt solution; Preferably, a method for raising cucumber seedlings comprises the following steps: soaking the seeds with a 10 mg / L S-adenosylmethionine salt solution, then planting and raising seedlings, and spraying the seedlings with a 10 mg / L S-adenosylmethionine salt solution; Preferably, a method for raising tomato seedlings comprises the following steps: soaking the seeds in a 20 mg / L S-adenosylmethionine salt solution, then planting and raising seedlings, and spraying the seedlings with a 20 mg / L S-adenosylmethionine salt solution.
9. The method according to any one of claims 6 to 8, characterized in that: In the method, the seeds are soaked in S-adenosylmethionine salt solution for 20-24 hours and then washed with water.
10. The method according to any one of claims 6 to 8, characterized in that: In the method, when the seedlings grow to two leaves and one heart, the whole seedlings are sprayed; Preferably, when the seedlings grow to two leaves and one heart, the whole seedling is sprayed once; Preferably, 80-100 mL of S-adenosylmethionine salt solution is sprayed on each plant; Preferably, in the method, the solvent of the S-adenosylmethionine salt solution is water; Preferably, the solvent of the S-adenosylmethionine salt solution is deionized water.