A fungicidal composition for preventing and treating tomato fungal diseases and application thereof

Abstract

This invention relates to the field of pesticide technology, and more particularly to a fungicidal composition and its application for controlling fungal diseases of tomatoes. The fungicidal composition provided by this invention comprises trifluoropyridineamine and biguanide trioctylbenzene sulfonate as its active ingredients. The fungicidal composition and pesticide formulations containing it not only have good control effects on tomato gray leaf spot, but also on tomato gray mold. This synergistic combination improves the disease control effect of chemical agents, achieving multiple protections with a single spray, effectively reducing the amount of chemical agents used, improving control efficiency, and achieving the goal of reducing pesticide use while increasing efficiency, thus increasing yield and income for vegetable farmers.

Description

Technical Field

[0001] This invention relates to the field of pesticide technology, and in particular to a fungicidal composition for controlling fungal diseases of tomatoes and its application. Background Technology

[0002] The widespread cultivation of greenhouse tomatoes ensures a year-round supply, playing a vital role in enriching people's diets and ensuring food security. However, the high humidity and suitable temperatures within greenhouses create ideal conditions for disease occurrence and development. Diseases such as gray mold and gray leaf spot severely impact the yield and quality of greenhouse tomatoes.

[0003] Gray leaf spots on tomatoes are caused by the genus *Pseudomonas* (…). Stemphylium (Spp.) is an important disease caused by a fungus. It primarily affects the leaves, typically starting on the lower and middle leaves. Initially, the leaf surface is covered with small, dark green, round or nearly round spots, which gradually expand outwards. Later, limited by the veins, these spots become polygonal, and the center gradually fades to grayish-white to grayish-brown. The lesions are slightly sunken and easily rupture, perforating and eventually falling off. In severe cases, lesions cover the entire leaf, sometimes merging into irregular patches, causing the leaves to dry out and fall off, and even causing the entire branch to yellow and wither. Severe outbreaks can also damage petioles, stems, and even fruits, resulting in yield losses.

[0004] Tomato gray mold is caused by Botrytis cinerea (… Botrytis cinerea Gray mold is a global disease caused by infection. It can infect all above-ground parts of tomato plants, primarily affecting fruits and leaves. Fruit infection often begins with residual petals and stigmas, causing flower rot, and then spreads to the fruit surface and stalk. Symptoms usually appear first near the fruit stem, stalk, or blossom end. Before ripening, the diseased areas of the fruit peel turn grayish-white, develop into water-soaked soft rot, and quickly form large, irregular spots. After the fruit loses water, it becomes stiff or moist and soft. Diseased fruits generally do not fall off; they spread through contact, and in severe cases, the entire bunch of fruits will rot. Numerous conidia are produced at the infected sites. Leaf infection often begins at the leaf tip, expanding inward in an inverted "V" shape. The lesions initially appear water-soaked with irregular edges, later turning light brown to yellowish-brown with alternating light and dark concentric rings. Infected petals and stamens falling onto the leaves can form circular or spindle-shaped lesions. Generally, the stems are less affected. When damaged, the disease initially appears as water-soaked spots, which later expand into elongated oval or striped spots. When the lesions encircle the stem, the upper branches and leaves wilt and die. In humid conditions, a dense layer of grayish-brown mold (commonly known as "mouse skin") grows on the affected fruit, leaves, and stems, gradually giving rise to black sclerotia of varying sizes within the mold. Gray mold typically causes yield losses of 10% to 20%, and in severe cases, losses can exceed 60%, resulting in significant economic losses.

[0005] Gray mold and gray leaf spot are important diseases in greenhouse tomato production. In production, the control of these two diseases mainly relies on spraying chemical fungicides. However, highly effective and low-toxicity fungicides usually have a single site of action and a high risk of resistance. How to delay the development of drug resistance in pathogens, extend the lifespan of highly effective fungicides, and achieve multi-layered protection with a single spray can not only reduce control costs and improve control efficiency but also reduce the amount of chemical agents used. This is currently a hot research topic and a challenge.

[0006] In view of this, the present invention is hereby proposed. Summary of the Invention

[0007] To address the aforementioned technical problems, this invention provides a fungicidal composition for preventing and controlling fungal diseases in tomatoes and its application.

[0008] Specifically, the technical solution of the present invention is as follows:

[0009] In a first aspect, the present invention provides a bactericidal composition, the active components of which are trifluoropyridineamine and biguanide trioctylbenzene sulfonate, wherein the mass ratio of trifluoropyridineamine to biguanide trioctylbenzene sulfonate is 1:(1-5).

[0010] Preferably, the mass ratio of trifluoropyridineamine to biguanide trioctylbenzene sulfonate is 1:1.

[0011] Secondly, the present invention provides a pesticide formulation containing the aforementioned bactericidal composition, wherein the bactericidal composition accounts for 1%-20% of the pesticide formulation.

[0012] Preferably, the pesticide formulation is a nano-aqueous suspension.

[0013] Preferably, the pesticide formulation comprises the following components: 2-4 parts by weight of trifluoropyridineamine, 2-4 parts by weight of biguanide trioctylbenzene sulfonate, 40-60 parts by volume of dichloromethane, 2-4 parts by weight of cellulose, 2-4 parts by weight of emulsifying dispersant, and 150-250 parts by volume of water; wherein, when the unit of the parts by weight is g, the unit of the parts by volume is mL.

[0014] More preferably, the emulsifying dispersant is sodium dodecyl sulfate and agricultural emulsion 600 in a mass ratio of 1:1-3.

[0015] Preferably, the pesticide formulation further includes an adjuvant component, which includes: 0.5-1.5 parts by weight of ethylene glycol and 1-3 parts by weight of nano-silica.

[0016] More preferably, the pesticide formulation comprises the following components: 3 parts by weight of trifluoropyridineamine, 3 parts by weight of biguanide trioctylbenzene sulfonate, 50 parts by volume of dichloromethane, 3 parts by weight of cellulose, 3 parts by weight of emulsifying dispersant, 200 parts by volume of water, 1 part by weight of ethylene glycol, and 2 parts by weight of nano-silica; wherein, when the unit of the parts by weight is g, the unit of the parts by volume is mL.

[0017] Thirdly, the present invention provides a method for preparing the pesticide formulation, comprising: mixing trifluoropyridineamine, biguanide trioctylbenzene sulfonate, dichloromethane and cellulose to obtain an oil phase; mixing an emulsifying dispersant and water to obtain an aqueous phase; mixing the oil phase and the aqueous phase, emulsifying and removing dichloromethane to obtain a nano-pesticide mother liquor; and adding adjuvant components to the nano-pesticide mother liquor.

[0018] Fourthly, the present invention provides the application of the fungicidal composition, the pesticide formulation, or the pesticide formulation prepared by the method described in the claims in the prevention and control of gray leaf spot and gray mold in tomatoes.

[0019] Fifthly, the present invention provides a method for using the fungicidal composition, the pesticide formulation, or the pesticide formulation prepared by the preparation method, wherein the application rate of the active ingredient is controlled at 157.5~300 g / ha, the application interval is 10±3 days, and the number of applications is 3±1.

[0020] Beneficial effects:

[0021] This invention provides a fungicide composition whose active ingredients are trifluoropyridineamine and biguanide trioctylbenzene sulfonate. The fungicide composition and pesticide formulations containing it provided by this invention not only have good control effects on tomato gray leaf spot, but also on tomato gray mold. This synergistic combination improves the disease control effect of chemical agents, achieving multiple protections with a single spray, effectively reducing the amount of chemical agents used, improving control efficiency, and achieving the goal of reducing pesticide use while increasing efficiency, and increasing yield and income for vegetable farmers. Detailed Implementation

[0022] This invention provides a compound synergistic fungicide composition for controlling fungal diseases in tomatoes. Indoor combined toxicity tests show that the fungicide composition of this invention has good inhibitory activity against both *Botrytis cinerea* and *Botrytis cinerea*, with a significant synergistic effect. Simultaneously, field efficacy tests show that the fungicide composition of this invention has good control efficacy against tomato gray leaf spot and gray mold, achieving multiple protections with a single spray, reducing the number of applications, effectively reducing the amount of chemical agents used, improving control efficiency, and achieving the goal of reducing chemical pesticide use while increasing efficacy.

[0023] The bactericidal composition of the present invention comprises trifluoropyridineamine and biguanide trioctylbenzene sulfonate as active components.

[0024] Trifluopyridine is a new generation of succinate dehydrogenase inhibitor (SDHI) fungicide developed and launched by Syngenta. It is another fungicide / nematicide in this class of products after Bayer fluopyram, and it is also the second compound with a nicotinamide structure (or pyridineamide structure) after BASF diazinon. This agent not only has nematicidal activity, but also has good control effect on diseases such as wheat stem rot and Fusarium head blight caused by Fusarium.

[0025] Biguanide trioctylbenzene sulfonate is a broad-spectrum fungicide with strong local penetration. It is a contact and preventative fungicide, primarily acting on the synthesis of lipid compounds and cell membrane function in pathogens, inhibiting spore germination, germ tube elongation, appressorium formation, and hyphae formation. Currently, it is registered in my country for the control of diseases such as asparagus stem blight, watermelon vine blight, gray mold in grapes and tomatoes, and powdery mildew in cucumbers.

[0026] Our team has discovered through extensive research that trifluoropyridinamide and biguanide trioctylbenzenesulfonate exhibit good antibacterial activity against tomato gray leaf spot pathogen. To delay the development of resistance to trifluoropyridinamide, our team conducted a study on the combined toxicity of trifluoropyridinamide and biguanide trioctylbenzenesulfonate in different proportions against tomato gray leaf spot pathogen. Unexpectedly, during the research process, we discovered that the combination of trifluoropyridinamide and biguanide trioctylbenzenesulfonate not only enhances the toxicity against tomato gray leaf spot pathogen but also demonstrates good control efficacy in the field and a good control effect against tomato gray mold. This achieves multiple protections with a single spray, improves control efficiency, and achieves the effect of reducing chemical pesticide application while increasing efficiency.

[0027] To further enhance the combined toxicity of the bactericidal composition, this invention also studies the specific compounding method of the active components, as follows:

[0028] In the active component, the mass ratio of trifluoropyridineamine to biguanide trioctylbenzenesulfonate is 1:(1-5). By controlling the ratio of the two, the combined toxicity of the composition can be significantly improved while controlling costs.

[0029] More preferably, the mass ratio of trifluoropyridine to biguanide trioctylbenzene sulfonate is 1:1, which can significantly improve the combined toxicity of the composition against tomato gray leaf spot and gray mold while controlling costs.

[0030] A second aspect of the present invention provides a pesticide formulation containing the above-mentioned bactericidal composition.

[0031] Specifically, the pesticide formulation is a nano-aqueous suspension.

[0032] The pesticide formulation described in this invention not only maintains a good control effect on tomato gray leaf spot, but also has a good control effect on tomato gray mold, achieving multiple preventions with one spray, improving control efficiency, and achieving the goal of increasing vegetable farmers' production and income.

[0033] The active ingredient accounts for 1%-20% of the total mass of the pesticide formulation. The specific amount can be adjusted according to actual needs.

[0034] The pesticide formulation is a nano-aqueous suspension, but it can also be formulated as a wettable powder, emulsifiable concentrate, or other formulations as needed.

[0035] The third aspect of the present invention provides a method for preparing the pesticide formulation, comprising: mixing trifluoropyridineamine, biguanide trioctylbenzene sulfonate, dichloromethane and cellulose to obtain an oil phase; mixing an emulsifying dispersant and water to obtain an aqueous phase; mixing the oil phase and the aqueous phase, emulsifying and removing dichloromethane to obtain a nano-pesticide mother liquor; and adding adjuvant components to the nano-pesticide mother liquor.

[0036] In one more specific and preferred embodiment, the preparation method of the pesticide formulation as a suspension includes the following steps: 3 g of trifluoropyridineamine and 3 g of biguanide trioctylbenzene sulfonate are dissolved in 50 mL of dichloromethane, an organic solvent. 3 g of cellulose is added to obtain an oil phase. 3 g of emulsifying dispersant (1 g of sodium dodecyl sulfate and 2 g of agricultural emulsion 600) is dissolved in 200 mL of water to obtain an aqueous phase. The oil and aqueous phases are mixed and emulsified using an ultrasonic pulverizer for 20 min to obtain a primary emulsion. The primary emulsion is homogenized under high pressure for 15 min using a high-pressure homogenizer to obtain a fine emulsion. The fine emulsion is stirred in an electric stirrer for 24 h (1200 rpm) to evaporate the organic solvent, obtaining a nano-pesticide mother liquor. 1 g of ethylene glycol and 2 g of nano-silica are added to the nano-pesticide mother liquor, and the mixture is ground using 0.3-0.4 mm diameter 95 zirconium beads to finally obtain a nano-pesticide aqueous suspension.

[0037] A fourth aspect of this invention provides the application of the fungicidal composition or the pesticide formulation in the control of gray leaf spot and gray mold in tomatoes. Experiments show that implementing the fungicidal composition or the pesticide formulation of this invention can significantly improve field control effects, achieve multiple preventions with a single spray, significantly reduce the amount of chemical agents used, and achieve the goal of increasing yields and income for vegetable farmers.

[0038] The fifth aspect of this invention provides a method for using the fungicidal composition, the pesticide formulation, or the pesticide formulation prepared by the method, wherein the application rate of the active ingredient is controlled at 157.5~300 g / ha, the application interval is 10±3 days, and the number of applications is 3±1. The method of use provided by this invention maximizes its medicinal activity without causing significant phytotoxicity to farmland and crops.

[0039] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without creative effort are within the scope of protection of this invention.

[0040] The endpoints and any values ​​of the ranges disclosed in this specification are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of the various ranges, the endpoint values ​​of the various ranges and individual point values, and individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed herein.

[0041] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "specific implementation," or "some specific implementations," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0042] In the embodiments provided in this specification, unless specific techniques or conditions are specified, the techniques or conditions described in the literature in this field, or the product instructions, shall be followed. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased from legitimate channels.

[0043] In the following examples: Trifluoropyridine amine suspension concentrate, with an active ingredient concentration of 450 g / L, was obtained commercially. Biguanide trioctylbenzene sulfonate wettable powder, with an active ingredient concentration of 40%, was obtained commercially. Biguanide trioctylbenzene sulfonate, with an active ingredient concentration of 90%, was obtained commercially.

[0044] Example 1

[0045] This embodiment provides a nano-pesticide formulation, specifically a 30 g / L trifluoropyridineamine·biguanide trioctylbenzene sulfonate nano-suspension (1:1).

[0046] This embodiment provides a method for preparing the above-mentioned nano-pesticide formulation: 3 g of trifluoropyridineamine and 3 g of biguanide trioctylbenzene sulfonate are dissolved in 50 mL of dichloromethane, an organic solvent. 3 g of cellulose is added to obtain an oil phase. 3 g of emulsifying dispersant (1 g of sodium dodecyl sulfonate and 2 g of agricultural emulsion 600) is dissolved in 200 mL of water to obtain an aqueous phase. The oil and aqueous phases are mixed and emulsified for 20 min using an ultrasonic pulverizer to obtain a primary emulsion. The primary emulsion is homogenized under high pressure for 15 min using a high-pressure homogenizer to obtain a fine emulsion. The fine emulsion is stirred for 24 h (1200 rpm) using an electric stirrer to evaporate the organic solvent, obtaining a nano-pesticide mother liquor. 1 g of ethylene glycol and 2 g of nano-silica are added to the above nano-pesticide mother liquor, and the mixture is ground using 95 zirconium beads with a diameter of 0.3-0.4 mm to finally obtain a nano-pesticide aqueous suspension.

[0047] Effect verification

[0048] To further verify that the bactericidal composition obtained by the present invention has a good bactericidal effect, the following experimental examples are used to illustrate this. Due to space limitations, the present invention only records convincing formulations and experiments.

[0049] In the following test examples: trifluoropyridine amine suspension, with an active ingredient concentration of 450 g / L, was obtained commercially. Biguanide trioctylbenzene sulfonate wettable powder, with an active ingredient concentration of 40%, was obtained commercially. Biguanide trioctylbenzene sulfonate, with an active ingredient concentration of 90%, was obtained commercially. All compound test reagents involved in the following test examples were prepared from the formulations in the above examples.

[0050] Experimental Example 1

[0051] This experimental example illustrates the combined virulence of trifluoropyridine and biguanide trioctylbenzene sulfonate in inducing major tomato pathogens.

[0052] The combined virulence of trifluoropyridine amine and biguanide trioctylbenzene sulfonate tomato gray leaf spot causal agent and gray mold was determined by the mycelial growth rate method.

[0053] The tested pathogenic strains were *Hypericum globosum* (HSR3) and *Botrytis cinerea* (BDD2), which were preserved by the Fungicide Research Laboratory of the Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences.

[0054] The test strains were pre-cultured using PDA medium. The PDA medium consisted of 200g potato, 20g glucose, 10g agar, and 1000mL distilled water.

[0055] The test drug was dissolved in acetone to prepare a stock solution with a concentration of 10,000 mg / L for later use. The formulation was directly dissolved in water to support the 10,000 mg / L stock solution. Trifluoropyridine and biguanide trioctylbenzene sulfonate were mixed at different mass ratios (1:1, 1:3, and 1:5) to obtain mixtures with different proportions. These were then serially diluted with sterile water and mixed with culture medium at a ratio of 1:9 to prepare PDA plates containing drug at various concentrations. A 5 mm diameter mycelial disc was collected from the edge of the pre-cultured strain and inoculated face down into the center of the drug-containing plate. The plates were incubated at 24 °C. When the control colony expanded to approximately 80% of the plate's volume, the diameter of each treatment and the control was measured using the cross-crossing method. EC was calculated using DPS software. 50 And correlation coefficient.

[0056] Calculation of inhibition rate:

[0057] Inhibition rate (%) = [(Control colony growth - Treatment colony growth germination rate) / Control colony growth] × 100%.

[0058] Calculate the synergistic effect coefficient of the drug mixture using the Wadley formula:

[0059]

[0060] SR=EC 50 (th) / EC 50 (ob)

[0061] In the above formula, a and b represent the proportions of the two bactericides in the mixture; EC 50 (A) and EC 50 (B) represents the actual observations of EC for A and B, respectively. 50 Value, EC 50 (th) represents the theoretical EC value of a mixture of fungicides A and B in a:b ratio. 50 Value, EC 50 (ob) represents the actual EC value after mixing fungicides A and B in a:b ratio. 50 Value. SR>1.5 indicates synergistic effect; SR<0.5 indicates antagonistic effect; SR between 0.5 and 1.5 indicates additive effect.

[0062] The test results are shown in the table below:

[0063]

[0064] Table 1 shows that the combination of trifluoropyridine and biguanide trioctylbenzene sulfonate showed good inhibitory effects on the mycelial growth of tomato gray leaf spot pathogen. The synergistic coefficients of each ratio (1:1-1:5) were all greater than 1.5, showing a synergistic effect on toxicity. When the ratio of the two was 1:1, the synergistic coefficient was the largest, which was 3.0.

[0065]

[0066] Table 2 shows that the combination of trifluoropyridine and biguanide trioctylbenzene sulfonate both showed good inhibitory effects on the mycelial growth of tomato gray mold. When the ratio of the two was 1:1 and 1:5, the synergistic coefficient was greater than 1.5, showing a synergistic effect on toxicity. When the ratio of the two was 1:3, the synergistic coefficient was 1.2, showing an additive effect on toxicity.

[0067] Experimental Example 2

[0068] This experimental example illustrates the field efficacy of a mixture of trifluoropyridine and biguanide trioctylbenzene sulfonate against gray leaf spot in tomatoes.

[0069] The experiment was conducted in accordance with the "Field Efficacy Test Guidelines for Pesticides" GB / T17980.111-2004 "Field Efficacy Test Guidelines for Fungicides in the Control of Tomato Leaf Mold" issued by the Institute for Pesticide Control, Ministry of Agriculture and Rural Affairs.

[0070] The experimental field was located in Xihe Village, Liuchu Town, Raoyang County, Hebei Province. The tomato variety was Lola. The first application of pesticide was started before the occurrence of gray leaf spot on the tomatoes. The pesticide was applied three times consecutively, with an interval of about 10 days between applications. The disease incidence was investigated in the field 10 days after the last application, and the disease index and control effect were calculated.

[0071]

[0072] The experiment showed that a 1:1 mixture of 450 g / L trifluoropyridine amine suspension and 40% biguanide trioctylbenzene sulfonate wettable powder exhibited good control effects on tomato gray leaf spot. Compared with single chemical agents, the amount of chemical agent used was significantly reduced, by 20%, and no adverse effects on crops or other beneficial organisms were observed at the tested dosage.

[0073] Spraying with 350 mL / mu of 30 g / L trifluoropyridineamine·biguanide trioctylbenzenesulfonate nano-formulation showed an efficacy of 86.89% against tomato gray leaf spot. This efficacy was not significantly different from that of single-agent formulations of 30 mL / mu of 450 g / L trifluoropyridineamine suspension and 50 g / mu of 40% biguanide trioctylbenzenesulfonate wettable powder, although the dosage was reduced by 22.2%. Spraying with 450 mL / mu of 30 g / L trifluoropyridineamine·biguanide trioctylbenzenesulfonate nano-formulation showed an efficacy of 90.09% against tomato gray leaf spot, significantly higher than that of single-agent formulations of 30 mL / mu of 450 g / L trifluoropyridineamine suspension and 50 g / mu of 40% biguanide trioctylbenzenesulfonate wettable powder. All tested agents had no adverse effects on crops or other beneficial organisms at the tested dosages.

[0074] Experimental Example 3

[0075] This experimental example illustrates the field control efficacy of trifluopyridine with other agents and their compound formulations against tomato gray mold.

[0076] The experiment was conducted in accordance with the "Field Efficacy Test Guidelines for Pesticides" GB / T17980.28-2000 "Efficacy Test Guidelines for Fungicides in the Control of Gray Mold in Vegetables" issued by the Institute for Pesticide Control, Ministry of Agriculture and Rural Affairs.

[0077] The experimental field was located in Baitapu Village, Xushui District, Baoding City, Hebei Province. During the season when gray mold of tomatoes is common, the first application of pesticide was carried out before the onset of gray mold. The pesticide was applied three times consecutively, with an interval of about 7 days between applications. Nine days after the last application, the disease incidence in the field was investigated, and the disease index and control effect were calculated.

[0078]

[0079] The experiment showed that a 1:1 mixture of 450 g / L trifluoropyridine amine suspension and 40% biguanide trioctylbenzene sulfonate wettable powder exhibited good control efficacy against tomato gray mold. Compared with single chemical agents, the dosage of chemical agents was significantly reduced by 20% to achieve similar control effects, and no adverse effects on crops or other beneficial organisms were observed at the tested dosage.

[0080] The nano-formulation 30 g / L trifluoropyridinamide·biguanide trioctylbenzenesulfonate suspension at 350 mL / mu showed an efficacy of 85.74% against tomato gray mold, which was not significantly different from the efficacy of single-agent formulations of 450 g / L trifluoropyridinamide suspension at 30 mL / mu and 40% biguanide trioctylbenzenesulfonate wettable powder at 50 g / mu, but the dosage was reduced by 22.2%. The nano-formulation 30 g / L trifluoropyridinamide·biguanide trioctylbenzenesulfonate suspension at 450 mL / mu showed an efficacy of 89.30% against tomato gray mold, significantly higher than the efficacy of single-agent formulations of 450 g / L trifluoropyridinamide suspension at 30 mL / mu and 40% biguanide trioctylbenzenesulfonate wettable powder at 50 g / mu. The efficacy of the nano-formulation 30 g / L trifluoropyridinamide·biguanide trioctylbenzenesulfonate suspension at 350 mL / mu and 450 g / mu was also significantly higher than that of single-agent formulations of 450 g / L trifluoropyridinamide suspension at 30 mL / mu and 40% biguanide trioctylbenzenesulfonate wettable powder at 50 g / mu. The two treatments at a dose of mL / mu were significantly more effective than the conventional fungicide 50% boscalid water-dispersible granules at 48 g / mu in controlling tomato gray mold. All tested fungicides showed no adverse effects on crops or other beneficial organisms at the tested doses.

[0081] Based on the above experiments, it can be seen that the combination of trifluoropyridine amine and biguanide trioctylbenzene sulfonate, or the preparation of a nano-pesticide 30g / L trifluoropyridine amine·biguanide trioctylbenzene sulfonate suspension, can simultaneously control multiple diseases such as tomato gray leaf spot and gray mold, achieving multiple preventions with one spray, effectively reducing the amount of chemical agents used, improving control efficiency, and achieving the goal of increasing vegetable farmers' yield and income.

[0082] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. The application of a fungicide composition in the control of gray leaf spot and gray mold in tomatoes, characterized in that, Its active components are trifluoropyridineamine and biguanide trioctylbenzene sulfonate, with a mass ratio of trifluoropyridineamine to biguanide trioctylbenzene sulfonate of 1:(1-5).

2. The application according to claim 1, characterized in that, The mass ratio of trifluoropyridineamine to biguanide trioctylbenzene sulfonate is 1:

1.

3. The application of a pesticide formulation in the control of gray leaf spot and gray mold in tomatoes, characterized in that, It contains the bactericidal composition according to claim 1 or 2, and the bactericidal composition accounts for 1%-20% of the pesticide formulation.

4. The application according to claim 3, characterized in that, The pesticide formulation is a nano-aqueous suspension.

5. The application according to claim 4, characterized in that, The pesticide formulation comprises the following components: 2-4 parts by weight of trifluoropyridineamine, 2-4 parts by weight of biguanide trioctylbenzene sulfonate, 40-60 parts by volume of dichloromethane, 2-4 parts by weight of cellulose, 2-4 parts by weight of emulsifying dispersant, and 150-250 parts by volume of water; wherein, when the unit of the parts by weight is g, the unit of the parts by volume is mL.

6. The application according to claim 5, characterized in that, The emulsifying dispersant is sodium dodecyl sulfate and agricultural emulsion 600 in a mass ratio of 1:1-3.

7. The application according to claim 5, characterized in that, The pesticide formulation also includes adjuvant components, which include: 0.5-1.5 parts by weight of ethylene glycol and 1-3 parts by weight of nano-silica.

8. The application according to claim 7, characterized in that, The pesticide formulation comprises the following components: 3 parts by weight of trifluoropyridineamine, 3 parts by weight of biguanide trioctylbenzene sulfonate, 50 parts by volume of dichloromethane, 3 parts by weight of cellulose, 3 parts by weight of emulsifying dispersant, 200 parts by volume of water, 1 part by weight of ethylene glycol, and 2 parts by weight of nano-silica; wherein, when the unit of the parts by weight is g, the unit of the parts by volume is mL.

9. The application according to any one of claims 3-8, characterized in that, The preparation method of the pesticide formulation includes: mixing trifluoropyridineamine, biguanide trioctylbenzene sulfonate, dichloromethane and cellulose to obtain an oil phase; mixing an emulsifying dispersant and water to obtain an aqueous phase; mixing the oil phase and the aqueous phase, emulsifying and removing dichloromethane to obtain a nano-pesticide mother liquor; and adding adjuvant components to the nano-pesticide mother liquor.

10. A method for controlling tomato gray leaf spot and gray mold, characterized in that, Using the fungicidal composition according to claim 1 or 2, or the pesticide formulation according to any one of claims 3-8, the application rate of the active ingredient is controlled at 157.5~300 g / ha, the application interval is 10±3 days, and the number of applications is 3±1.

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