Composition with effects of improving immunity and enhancing physical strength, preparation, food and application

Abstract

The invention provides a composition with effects of improving immunity and enhancing physical strength, preparation, food and application, and relates to the technical field of food health care. The composition comprises the following components: protamine, maca powder, a traditional Chinese medicine extract, raspberry powder, polysaccharide and collagen, the traditional Chinese medicine extract is American ginseng, medlar and andrographis paniculata; the mass ratio of the American ginseng to the fructus lycii to the herba andrographitis is (2-6): (2-4): (2-4) According to the composition provided by the invention, through the specific components, proportion and preparation method, the obtained product not only has the effects of remarkably improving immunity and anti-aging ability, but also is good in mouth feel.

Description

Technical Field

[0001] The invention relates to the technical field of food health care, and in particular to a composition having the effects of improving immunity and enhancing physical strength, and its preparation, food and application. Background Art

[0002] Aging is a natural process, the result of long-term interaction between organisms and the external environment. With the increase of age, human body functions begin to decline, which is manifested as structural and functional decline, and decreased adaptability and resistance. The more obvious manifestations of aging are: osteoporosis caused by calcium loss, cardiovascular and cerebrovascular diseases and stroke caused by vascular sclerosis, decreased immune function, decreased sexual function, etc. The incidence of cardiovascular and cerebrovascular diseases such as myocardial infarction and cerebral hemorrhage in middle-aged people has increased significantly, and the incidence of diseases such as coronary heart disease, hypertension, hyperlipidemia, and diabetes has also tended to be younger. People are constantly looking for ways to live a healthy and long life, improve immunity, overcome various diseases, and improve physical functions to improve the quality of life. Therefore, it is necessary to continuously develop nutritious and health foods that are easy to be absorbed and utilized by the human body and have anti-aging, immunity and resistance-enhancing medicinal and edible properties, which have become a technical problem that technicians in this field need to solve urgently.

[0003] Protamine is a polycationic antibacterial short peptide found in fish sperm nuclei. It is a small and simple spherical basic protein with a small molecular weight, usually below 10,000. It is generally composed of about 30 amino acids. The molecule is rich in proline and arginine, of which more than 2 / 3 is arginine. The isoelectric point is 10-12. It is soluble in water and dilute acid and does not coagulate when heated. As a protein substance rich in arginine, protamine has high nutritional and functional properties, such as lowering blood pressure, aiding breathing, promoting digestion, inhibiting tumors, anti-thrombosis, strengthening liver function, and inhibiting blood coagulation. In addition, protamine has also been widely used in the medical field, such as delaying or preventing the release of insulin, or as an antidote for heparin. Researchers in this field have studied the effects of protamine in improving immunity and anti-aging.

[0004] For example, "The Effect of Protamine-Coated PLA Nanocapsules on the Immune Function of Mouse Bone Marrow Dendritic Cells" (Journal of Huazhong University of Science and Technology (Medical Edition), Yang Ping, Zhu Junming, Vol. 45, No. 3, P298) disclosed the effect of protamine (PS)-coated polylactic acid (PLA) nanocapsules on the in vitro immune response of mouse bone marrow dendritic cells (BMDC). However, this study mainly utilized the property of protamine as a positively charged and biodegradable material, and did not study the direction of protamine as a nutrient itself and its ability to improve immunity.

[0005] Chinese patent application CN108309834A discloses a skin care composition based on brown algae extract, including methylsilanol mannuronate, dimethylsilanol, brown algae extract, nano zinc powder, protamine, beeswax and water. The skin care composition based on brown algae extract, with brown algae extract as the main active ingredient, especially when brown algae extract and protamine are used together, can play a certain nutritional supplement role on the skin and is conducive to repairing damaged cells. This reflects the application of protamine in the preparation of skin care products with anti-aging, repair and other effects, but it is also not a study on the effect of protamine as a nutrient, applied in the field of food and health care, and improving immunity.

[0006] In summary, developing an edible composition with protamine as the main active ingredient, which has significant effects in improving immunity, anti-aging, and strengthening the body, is the research focus of researchers in this field. Summary of the invention

[0007] In view of the above problems, the present invention provides a composition with the effects of improving immunity and enhancing physical strength. The composition takes protamine as a main component and works together with maca powder, Chinese medicine extract, raspberry powder, polysaccharide and collagen to prepare a composition with significant effects of improving immunity and anti-aging.

[0008] To achieve the above purpose, the technical solution adopted by the present invention is as follows: On the one hand, the present invention provides a composition with the effects of improving immunity and enhancing physical strength, comprising the following components: protamine, maca powder, Chinese medicine extract, raspberry powder, polysaccharide and collagen; the Chinese medicine extract is American ginseng, wolfberry and andrographis; the mass ratio of American ginseng, wolfberry and andrographis is 2-6:2-4:2-4.

[0009] Preferably, the mass ratio of the protamine, raspberry powder and polysaccharide is 8-12:1-3:0.1-2.

[0010] Further preferably, the mass ratio of the protamine, raspberry powder and polysaccharide is 10:2:1.

[0011] Preferably, the mass ratio of American ginseng, wolfberry and andrographis paniculata is 3-4:2-3:2-3.

[0012] More preferably, the mass ratio of American ginseng, wolfberry and andrographis paniculata is 4:3:3.

[0013] Preferably, the preparation method of the Chinese medicine extract is: Step 1) mixing the raw material with alcohol, heating and extracting for 1-3 times, combining the extracts to obtain an alcohol extract and a filter residue; Step 2) the filter residue is further leached with water for 1-3 times, and the water extract is combined; Step 3) The alcohol extract is combined with the water extract to concentrate the extract, dry it, and grind it.

[0014] Preferably, in step 1), the material-liquid ratio of the raw material to the alcohol is 1 g:8-12 L.

[0015] Further preferably, in step 1), the solid-liquid ratio of the raw material to the alcohol is 1 g:9-10 L; more preferably, in step 1), the solid-liquid ratio of the raw material to the alcohol is 1 g:9 L.

[0016] Preferably, in step 1), the alcohol is an ethanol solution with a volume fraction of 60%-75%.

[0017] Further preferably, in step 1), the alcohol is an ethanol solution with a volume fraction of 65%.

[0018] Preferably, in step 2), the water is selected from at least one of purified water, deionized water or distilled water; further preferably, in step 2), the water is purified water.

[0019] Preferably, in step 2), the material-liquid ratio of the filter residue to water is 1 g:8-12 L.

[0020] Further preferably, in step 2), the solid-liquid ratio of the filter residue to water is 1 g:9-10 L; more preferably, in step 2), the solid-liquid ratio of the filter residue to water is 1 g:10 L.

[0021] Preferably, in step 3), the relative density of the extract is 1-1.2.

[0022] Further preferably, the relative density of the extract is 1.

[0023] Preferably, the polysaccharide is Tricholoma matsutake polysaccharide.

[0024] Preferably, the preparation method of Tricholoma matsutake polysaccharide is: The pine mushroom powder is mixed with enzyme and water for enzymolysis; microwave extraction is performed, and the water extract is freeze-dried to obtain a concentrated product.

[0025] Preferably, the water is selected from at least one of purified water, deionized water or distilled water; more preferably, the water is purified water.

[0026] Preferably, the enzyme is selected from at least one of cellulase and papain; further preferably, the enzyme is a mixture of cellulase and papain.

[0027] Preferably, the dosage of the enzyme is 18-24 U / g Tricholoma matsutake powder.

[0028] More preferably, the dosage of the enzyme is 20 U / g Tricholoma matsutake powder.

[0029] Preferably, the pH of the enzymatic hydrolysis is 5-6 and the temperature is 50-60°C.

[0030] More preferably, the pH of the enzymatic hydrolysis is 5.5 and the temperature is 55°C.

[0031] Preferably, the frequency of the microwave extraction is 32-38 KHz.

[0032] Further preferably, the frequency of the microwave extraction is 34 KHz.

[0033] Further preferably, the preparation method of the Tricholoma matsutake polysaccharide is: wash, dry and crush the Tricholoma matsutake to obtain Tricholoma matsutake powder; add cellulase and papain, add water and stir evenly, adjust the pH value to 5-6, and perform enzymolysis at 50-60°C for 20-30 minutes; perform microwave extraction at a frequency of 32-38KHz, and freeze-dry the water extract to obtain a concentrated product.

[0034] Preferably, the composition comprises the following components in parts by mass: 80-120 parts of protamine, 10-20 parts of maca powder, 5-15 parts of traditional Chinese medicine extract, 10-30 parts of raspberry powder, 1-20 parts of polysaccharide and 15-30 parts of collagen.

[0035] Further preferably, the composition comprises the following components in parts by mass: 80-100 parts of protamine, 10-15 parts of maca powder, 10-15 parts of traditional Chinese medicine extract, 20-30 parts of raspberry powder, 1-10 parts of polysaccharide and 15-25 parts of collagen.

[0036] More preferably, the composition comprises the following components in parts by mass: 100 parts of protamine, 15 parts of maca powder, 10 parts of traditional Chinese medicine extract, 20 parts of raspberry powder, 10 parts of polysaccharide and 25 parts of collagen.

[0037] Preferably, the composition further comprises at least one of a flavoring agent or a fragrance.

[0038] Preferably, the flavoring agent is selected from at least one of D-mannitol, alitame, aspartame, advantame, erythrosine, xylitol or sucralose.

[0039] Preferably, the flavor is selected from at least one of sweet orange flavor, vanilla flavor, strawberry flavor, milk flavor, banana flavor or cherry flavor.

[0040] In another aspect, the present invention provides a method for preparing the above-mentioned composition, specifically comprising mixing the components in the composition.

[0041] In another aspect, the present invention provides the use of the above-mentioned composition in preparing food with immunity-enhancing and anti-aging effects.

[0042] In another aspect, the present invention provides a food having the effects of improving immunity and resisting aging, comprising the above-mentioned composition.

[0043] Preferably, the food is in the form of a formulation suitable for oral administration.

[0044] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention optimizes the components and preparation methods of Chinese medicine extracts, and finds in experiments that American ginseng, wolfberry, and andrographis paniculata have synergistic effects in improving immunity and anti-aging ability, especially under the specific ratio and preparation method of the present invention; 2. The present invention compounds protamine, maca powder, Chinese medicine extract, raspberry powder, pine mushroom polysaccharide and collagen to obtain a composition that can significantly improve immunity and anti-aging ability, especially under the specific ratio of the present invention, and protamine, raspberry powder and pine mushroom polysaccharide have a synergistic effect; 3. The composition prepared by the present invention not only has the effect of significantly improving immunity and anti-aging ability, but also has a good taste, which can significantly improve the acceptance of users. DETAILED DESCRIPTION

[0045] In order to make the technical means, creative features, purpose and effect of the present invention easy to understand, the present invention is further explained below in conjunction with specific embodiments, but the following embodiments are only preferred embodiments of the present invention, not all. Based on the embodiments in the implementation mode, other embodiments obtained by those skilled in the art without making creative work all belong to the protection scope of the present invention. It is worth noting that the raw materials used in the present invention are all common commercial products, and their sources are not specifically limited. The technology and scientific terms used in the embodiments have the meanings commonly understood by those of ordinary skill in the art to which the present invention belongs.

[0046] Example 1 A composition with the effects of improving immunity and enhancing physical strength comprises the following components: 100 parts of protamine, 15 parts of maca powder, 10 parts of Chinese herbal medicine extract, 20 parts of raspberry powder, 10 parts of pine mushroom polysaccharide, 25 parts of collagen, 3 parts of xylitol and 2 parts of cherry flavor essence.

[0047] (1) The Chinese herbal medicine extract is a mixture of American ginseng, wolfberry and andrographis paniculata, with the mass ratio of the three being 4:3:3.

[0048] The preparation method of the Chinese herbal medicine extract is as follows: Step 1) crushing the raw material, mixing it with 65% ethanol solution at a solid-liquid ratio of 1g:9L, heating to reflux extraction for 3 times, combining the extracts to obtain an alcohol extract and a filter residue; Step 2) The filter residue is further leached with purified water for 3 times, with a solid-liquid ratio of 1g:10L, and the water extract is obtained by combining; Step 3) The alcohol extract and the water extract are combined to concentrate the extract, freeze-dried, and crushed.

[0049] (2) The preparation method of Tricholoma matsutake polysaccharide is as follows: Wash, dry and crush the matsutake to obtain matsutake powder; add cellulase and papain (the amount of enzyme is 20U / g matsutake powder), add purified water and stir evenly, adjust the pH value to 5.5, and perform enzymolysis at 55°C for 30 minutes; perform microwave extraction at a frequency of 34KHz, and freeze-dry the water extract to obtain a concentrated product.

[0050] (3) The preparation method of the composition having the effects of improving immunity and enhancing physical strength is as follows: the components of the composition are mixed evenly and passed through a 40-100 mesh sieve.

[0051] Example 2 A composition having the effects of improving immunity and enhancing physical strength, which is different from Example 1 only in that the components are different: It contains the following components: 80 parts of protamine, 20 parts of maca powder, 15 parts of Chinese medicine extract, 30 parts of raspberry powder, 1 part of pine mushroom polysaccharide, 30 parts of collagen, 5 parts of xylitol, and 5 parts of cherry flavor.

[0052] The rest is the same as Example 1.

[0053] Example 3 A composition having the effects of improving immunity and enhancing physical strength, which is different from Example 1 only in that the components are different: It contains the following components: 120 parts of protamine, 10 parts of maca powder, 5 parts of Chinese medicine extract, 10 parts of raspberry powder, 20 parts of pine mushroom polysaccharide, 15 parts of collagen, 4 parts of xylitol, and 1 part of cherry flavor.

[0054] The rest is the same as Example 1.

[0055] Example 4 A composition having the effects of improving immunity and enhancing physical strength, which is different from Example 1 only in that the Chinese medicine extract is different: The Chinese herbal extract is a mixture of American ginseng, wolfberry and andrographis paniculata, with the mass ratio of the three being 3:2:2.

[0056] The rest is the same as Example 1.

[0057] Comparative Example 1 A composition having the effects of improving immunity and enhancing physical strength, which is different from Example 1 only in that the components are different: It contains the following components: 130 parts of protamine, 1 part of maca powder, 20 parts of Chinese medicine extract, 12 parts of raspberry powder, 0.5 parts of pine mushroom polysaccharide, 10.5 parts of collagen, 6 parts of xylitol, and 5 parts of cherry flavor.

[0058] The preparation method of the composition having the effects of improving immunity and enhancing physical strength is as follows: the components of the composition are mixed evenly and passed through a 40-100 mesh sieve.

[0059] The rest is the same as Example 1.

[0060] Comparative Example 2 A composition having the effects of improving immunity and enhancing physical strength, which is different from Example 1 only in that the components are different: 108 parts of protamine, 15 parts of maca powder, 10 parts of Chinese herbal medicine extract, 22 parts of raspberry powder, 25 parts of collagen, 3 parts of xylitol, and 2 parts of cherry flavor.

[0061] The preparation method of the composition having the effects of improving immunity and enhancing physical strength is as follows: the components of the composition are mixed evenly and passed through a 40-100 mesh sieve.

[0062] The rest is the same as Example 1.

[0063] Comparative Example 3 A composition having the effects of improving immunity and enhancing physical strength, which is different from Example 1 only in that the components are different: It contains the following components: 118 parts of protamine, 15 parts of maca powder, 10 parts of Chinese herbal medicine extract, 12 parts of pine mushroom polysaccharide, 25 parts of collagen, 3 parts of xylitol, and 2 parts of cherry flavor.

[0064] The preparation method of the composition having the effects of improving immunity and enhancing physical strength is as follows: the components of the composition are mixed evenly and passed through a 40-100 mesh sieve.

[0065] The rest is the same as Example 1.

[0066] Comparative Example 4 A composition having the effects of improving immunity and enhancing physical strength, which is different from Example 1 only in that the components are different: 100 parts of casein, 15 parts of maca powder, 10 parts of Chinese herbal medicine extract, 20 parts of raspberry powder, 10 parts of pine mushroom polysaccharide, 25 parts of collagen, 3 parts of xylitol, and 2 parts of cherry flavor.

[0067] The preparation method of the composition having the effects of improving immunity and enhancing physical strength is as follows: the components of the composition are mixed evenly and passed through a 40-100 mesh sieve.

[0068] The rest is the same as Example 1.

[0069] Comparative Example 5 A composition having the effects of improving immunity and enhancing physical strength, which is different from Example 1 only in that the preparation method of Tricholoma matsutake polysaccharide is different: Wash, dry and crush the matsutake mushrooms to obtain matsutake mushroom powder; add 15 times the mass of water to mix, heat to 90°C, soak for 3 hours, filter the extract, add 3 times the volume of 95% ethanol, precipitate for 3 hours, and obtain matsutake mushroom polysaccharide.

[0070] The rest is the same as Example 1.

[0071] Comparative Example 6 A composition having the effects of improving immunity and enhancing physical strength, the difference from Example 1 being that the polysaccharide of Tricholoma matsutake is replaced by polysaccharide of Ganoderma lucidum: The preparation method of the composition having the effects of improving immunity and enhancing physical strength is as follows: the components of the composition are mixed evenly and passed through a 40-100 mesh sieve.

[0072] The rest is the same as Example 1.

[0073] Comparative Example 7 A composition having the effects of improving immunity and enhancing physical strength, which is different from Example 1 only in that the Chinese medicine extract is different: (1) The Chinese herbal medicine extract is a mixture of American ginseng, wolfberry and andrographis paniculata, with the mass ratio of the three being 2:5:5.

[0074] The preparation method of the Chinese herbal medicine extract is as follows: Step 1) crushing the raw material, mixing it with 65% ethanol solution at a solid-liquid ratio of 1g:9L, heating to reflux extraction for 3 times, combining the extracts to obtain an alcohol extract and a filter residue; Step 2) The filter residue is further leached with purified water for 3 times, with a solid-liquid ratio of 1g:10L, and the water extract is obtained by combining; Step 3) The alcohol extract and the water extract are combined to concentrate the extract, freeze-dried, and crushed.

[0075] The rest is the same as Example 1.

[0076] Comparative Example 8 A composition having the effects of improving immunity and enhancing physical strength, which is different from Example 1 only in that the Chinese medicine extract is different: (1) The Chinese herbal extract is American ginseng.

[0077] The preparation method of the Chinese herbal medicine extract is as follows: Step 1) crushing the raw material, mixing it with 65% ethanol solution at a solid-liquid ratio of 1g:9L, heating to reflux extraction for 3 times, combining the extracts to obtain an alcohol extract and a filter residue; Step 2) The filter residue is further leached with purified water for 3 times, with a solid-liquid ratio of 1g:10L, and the water extract is obtained by combining; Step 3) The alcohol extract and the water extract are combined to concentrate the extract, freeze-dried, and crushed.

[0078] The rest is the same as Example 1.

[0079] Comparative Example 9 A composition having the effects of improving immunity and enhancing physical strength, which is different from Example 1 only in that the Chinese medicine extract is different: (1) The Chinese herbal extract is Andrographis paniculata.

[0080] The preparation method of the Chinese herbal medicine extract is as follows: Step 1) crushing the raw material, mixing it with 65% ethanol solution at a solid-liquid ratio of 1g:9L, heating to reflux extraction for 3 times, combining the extracts to obtain an alcohol extract and a filter residue; Step 2) The filter residue is further leached with purified water for 3 times, with a solid-liquid ratio of 1g:10L, and the water extract is obtained by combining; Step 3) The alcohol extract and the water extract are combined to concentrate the extract, freeze-dried, and crushed.

[0081] The rest is the same as Example 1.

[0082] Comparative Example 10 A composition having the effects of improving immunity and enhancing physical strength, which is different from Example 1 only in that the Chinese medicine extract is different: (1) The Chinese herbal extract is wolfberry.

[0083] The preparation method of the Chinese herbal medicine extract is as follows: Step 1) crushing the raw material, mixing it with 65% ethanol solution at a solid-liquid ratio of 1g:9L, heating to reflux extraction for 3 times, combining the extracts to obtain an alcohol extract and a filter residue; Step 2) The filter residue is further leached with purified water for 3 times, with a solid-liquid ratio of 1g:10L, and the water extract is obtained by combining; Step 3) The alcohol extract and the water extract are combined to concentrate the extract, freeze-dried, and crushed.

[0084] The rest is the same as Example 1.

[0085] Comparative Example 11 A composition having the effects of improving immunity and enhancing physical strength, which is different from Example 1 only in that the Chinese medicine extract is different: (1) The Chinese herbal medicine extract is a mixture of American ginseng, wolfberry and andrographis paniculata, with the mass ratio of the three being 4:3:3.

[0086] The preparation method of the Chinese medicine extract is as follows: mixing the above raw materials evenly.

[0087] Test Example 1 Immunity effect test: Experimental animals: 12-16g BALB / c young mice, provided by Chengdu Dashuo Biotechnology Co., Ltd., female, randomly divided into 15 groups, 10 mice in each group, namely, embodiment groups (Example 1-Example 4), comparative example groups (Comparative Example 1-Comparative Example 11) and control group; Experimental Design: Example group: 1.5 g of each composition prepared in Example 1 to Example 4 was dissolved in 200 mL of warm water.

[0088] Comparative Example Group: 1.5 g of each composition prepared in Comparative Example 1 to Comparative Example 11 was dissolved in 200 mL of warm water.

[0089] Control group: an equal dose of normal saline was administered orally.

[0090] Dosage: Each mouse was gavaged with 0.1 mL / g once, twice a day, for 5 consecutive weeks. The mice were killed 4 hours after the last gavage, and the immune organs were separated and weighed. The following analysis results were obtained, as shown in Table 1.

[0091] Detection indicators: mouse weight gain, thymus / body weight ratio, spleen / body weight ratio.

[0092] Table 1. Results of the immune effect test

[0093] Note: *P<0.05, **P<0.01 compared with the control group.

[0094] From the data in Table 1, it can be seen that the composition prepared in the embodiment of the present invention is administered to mice safely and has no toxic side effects, can significantly stimulate the growth of the immune organs of mice, and has a good effect of enhancing the immune function. Compared with the comparative example, the efficacy is obvious, indicating that only under the specific components, formulas and preparation methods of the present invention can the effect of significantly enhancing immunity be achieved.

[0095] Test Example 2 Anti-aging test Experimental Animals: Kunming mice (aged mice) aged 12 months or more, weighing 30-50 g, were randomly divided into 15 groups, 10 mice in each group, namely, embodiment groups (Example 1 to Example 4), comparative example groups (Comparative Example 1 to Comparative Example 11) and a control group; Experimental Design: Example group: The rats were fed with the compositions prepared in Examples 1 to 4, 3 times a day, 100 mg each time, for 45 consecutive days.

[0096] Comparative Example Group: The rats were fed with the compositions prepared in Comparative Example 1 to Comparative Example 11, 3 times a day, 100 mg each time, for 45 consecutive days.

[0097] Control group: an equal dose of normal saline was administered orally.

[0098] Detection: After 45 days, the erythrocyte superoxide dismutase (SOD) activity, heart and liver SOD activity and serum oxidized lipid (LPO) content of the mice in the control group, Example 1-Example 4 and Comparative Example 1-Comparative Example 11 were measured. Among them, the erythrocyte SOD enzyme activity was measured by the pyrogallol method, the heart and liver SOD was measured by the Lan method, and the blood oxidized lipid was measured by the modified Yagi Kunio method. The specific test results are shown in Table 2.

[0099] Table 2. Anti-aging test results

[0100] Note: *P<0.05, **P<0.01 compared with the control group.

[0101] From the data in Table 2, it can be seen that the composition prepared in the embodiment of the present invention is safe and has no toxic side effects when fed to mice, can significantly enhance the SOD activity of mice, reduce the LPO content, and has the effect of delaying aging. Compared with the comparative example, the efficacy is obvious, indicating that only under the specific components, formulas and preparation methods of the present invention can significantly better anti-aging effects be achieved.

[0102] Test Example 3 Taste test: Taste test of Example 1-Example 4 and Comparative Example 1-Comparative Example 11. The specific test method is to select 150 people, randomly divide them into 15 groups, 10 people in each group, and let them eat the compositions prepared according to Example 1-Example 4 and Comparative Example 1-Comparative Example 11, and evaluate and score the taste of the compositions, with a total score of 10 points.

[0103] Among them, 0=no taste, 1=slight taste / mouthfeel, 2=very weak taste / mouthfeel, 3=weak taste / mouthfeel, 4=weak taste / mouthfeel, 5=moderate taste / mouthfeel, 6=slightly better taste / mouthfeel, 7=better taste / mouthfeel, 8=good taste / mouthfeel, 9=very good taste / mouthfeel, and 10=very good taste / mouthfeel.

[0104] The scale includes the ability to use 0.1 points, thus having the possibility of 100 scale differences.

[0105] The results are shown in Table 3.

[0106] Table 3. Taste test results

[0107] It can be seen from the data in Table 3 that the compositions prepared in Examples 1 to 4 of the present invention have a very good taste. Although the Chinese herbal extract is added, it does not have a bad effect on the taste. By optimizing the preparation method of the Chinese herbal extract, the composition and ratio of the composition, especially the addition of raspberry powder, the taste is significantly improved and the user acceptance is improved.

[0108] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A composition having the effects of improving immunity and enhancing physical strength, characterized in that: The invention comprises the following components: protamine, maca powder, Chinese medicine extract, raspberry powder, polysaccharide and collagen; the Chinese medicine extract is American ginseng, wolfberry and andrographis paniculata; the mass ratio of American ginseng, wolfberry and andrographis paniculata is 2-6:2-4:2-4.

2. The composition according to claim 1, characterized in that The mass ratio of the protamine, raspberry powder and polysaccharide is 8-12:1-3:0.1-2.

3. The composition according to claim 2, characterized in that The mass ratio of the protamine, raspberry powder and polysaccharide is 10:2:

1.

4. The composition according to claim 1, characterized in that The mass ratio of American ginseng, wolfberry and andrographis paniculata is 3-4:2-3:2-3.

5. The composition according to claim 1, characterized in that The preparation method of the Chinese medicine extract is: Step 1) mixing the raw material with alcohol, heating and extracting for 1-3 times, combining the extracts to obtain an alcohol extract and a filter residue; Step 2) the filter residue is further leached with water for 1-3 times, and the water extract is combined; Step 3) The alcohol extract and the water extract are combined to concentrate the extract, dry it, and grind it.

6. The composition according to claim 5, characterized in that In step 1), the material-liquid ratio of the raw material to the alcohol is 1 g:8-12 L.

7. The composition according to claim 5, characterized in that In step 2), the material-liquid ratio of the filter residue to water is 1g:8-12L.

8. The composition according to claim 1, characterized in that The polysaccharide is Tricholoma matsutake polysaccharide.

9. The composition according to claim 8, characterized in that The preparation method of the Tricholoma matsutake polysaccharide comprises the following steps: mixing Tricholoma matsutake powder with enzyme and water, performing enzymolysis, performing microwave extraction, and freeze-drying the water extract to obtain a concentrated product.

10. The composition according to claim 9, characterized in that The enzyme is selected from at least one of cellulase and papain.

11. The composition according to claim 9, characterized in that The dosage of the enzyme is 18-24 U / g of pine mushroom powder.

12. The composition according to claim 9, characterized in that The pH of the enzymatic hydrolysis is 5-6, and the temperature is 50-60°C.

13. The composition according to any one of claims 1 to 12, characterized in that The composition comprises the following components by weight: 80-120 parts of protamine, 10-20 parts of maca powder, 5-15 parts of traditional Chinese medicine extract, 10-30 parts of raspberry powder, 1-20 parts of polysaccharide and 15-30 parts of collagen.

14. The composition according to claim 13, characterized in that The composition comprises the following components in parts by mass: 80-100 parts of protamine, 10-15 parts of maca powder, 10-15 parts of traditional Chinese medicine extract, 20-30 parts of raspberry powder, 1-10 parts of polysaccharide and 15-25 parts of collagen.

15. The composition according to claim 1, characterized in that The composition further comprises at least one of a flavoring agent or a fragrance.

16. A method for preparing the composition according to any one of claims 1 to 12, characterized in that: Specifically, the components in the composition are mixed.

17. Use of the composition according to any one of claims 1 to 12 in preparing food having immunity-enhancing and anti-aging effects.

18. A food with the effects of improving immunity and anti-aging, characterized in that: A composition comprising any one of claims 1 to 12.

19. The food according to claim 18, characterized in that The dosage form of the food is in the form of a formulation suitable for oral administration.

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