Tea polyphenol composition capable of controlling oil and removing acnes as well as preparation method and application of tea polyphenol composition

Abstract

The invention relates to the technical field of cosmetics, and discloses an oil-controlling acne-removing tea polyphenol composition, a preparation method and application thereof, the composition comprises 20-30 parts of tea polyphenol, 30-40 parts of capryloyl glycine and 50-60 parts of a solubilizing antioxidant; according to the composition, the normal-temperature water solubility of the capryloyl glycine is improved through the solubilizing antioxidant, the application range of the capryloyl glycine in water aqua is expanded, in addition, the solubilizing antioxidant can improve the oxidation resistance of the tea polyphenol and improve the stability of the tea polyphenol, the oil control and acne removal effects are remarkably improved when the composition is applied to water aqua cosmetics, and the product stability is high.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to an oil-controlling and acne-removing tea polyphenol composition, a preparation method and application thereof. Background Art

[0002] Acne, also known as "pimples", is a common chronic inflammatory skin disease with an incidence rate of more than 80% in adolescents. Adolescence is the most special stage in life. The body and organs begin to develop and gradually mature, the level of sex hormones increases, and the sebaceous glands increase their sebum secretion under the stimulation of androgens and other hormones. The increase in sebum secretion (that is, more oil secretion) causes the proliferation of lipophilic Propionibacterium acnes and other microorganisms in the hair follicles, which then decompose triglycerides into free fatty acids, and lipid peroxidation, leading to excessive keratinization of the hair follicle opening (the oil outlet of the hair follicle opening becomes narrower); then there is the accumulation of keratin and sebum in the hair follicles, resulting in acne; due to the accumulation of sebum, the local skin flora imbalance leads to mild inflammatory reactions in the hair follicle sebaceous gland unit, resulting in papules and pustules; if the inflammation is further amplified, the hair follicle wall ruptures, and the contents of the hair follicle enter the dermis, severe nodules or cysts will be formed, and when they merge into pieces, they may lead to the formation of post-acne scars. Therefore, excessive secretion of sebaceous glands is a prerequisite for the occurrence of acne.

[0003] The results of a study on the age of the skin from children to adolescents showed that in late adolescence (≥12 years old), the lipids on the skin surface increase significantly, the colonization of lipophilic microorganisms such as Propionibacterium acnes increases, metabolism is active, the microecological barrier is abnormal, acne is prone to occur and the barrier function is slightly damaged. At this time, it is not recommended to use strong acne-removing products. Improper use of cosmetics will increase the burden on the skin and lead to problems such as premature aging of sensitive skin.

[0004] Currently, the acne-removing products on the market mainly contain high doses of oil-control or antibacterial ingredients such as fruit acid, azelaic acid, salicylic acid, etc. Although these substances have extremely strong antibacterial activity, they can also cause strong irritation to the skin. Therefore, consumers with sensitive skin, especially teenagers, should not use products made with the above substances. Therefore, it is of great significance to prepare safe, mild, and low-irritation oil-control and acne-removing products.

[0005] The prior art discloses that tea polyphenols and capryloyl glycine have the effects of removing acne and reducing inflammation, such as the preparation method of an acne-repairing essence containing tea polyphenols disclosed in Publication No. CN108815092A, and another example is the high-content capryloyl glycine oil-controlling acne-removing composition disclosed in Publication No. CN117243834A, as well as its preparation method and application. Currently, there is a large amount of evidence indicating that tea polyphenols and capryloyl glycine are safe, mild and low-irritation oil-control products, and therefore tea polyphenols and capryloyl glycine can be used as important functional components of acne-removing products.

[0006] Although tea polyphenols and capryloyl glycine have excellent acne-removing effects, tea polyphenols still have the problems of easy oxidation and short shelf life, because they have phenolic hydroxyl groups in their molecules and are acidic in nature. The higher the pH, the worse the stability. In addition, when tea polyphenols are in an aqueous solution and polyphenol oxidase is present, the phenolic hydroxyl groups can be gradually oxidized to reddish-brown polymers through dissociation. In particular, skin care products containing tea polyphenols change color significantly under high temperature conditions, and the content of active ingredients decreases, affecting the performance of the product. The solubility of capryloyl glycine in room temperature water is only 1.557 g / L, and it has the problem of low degree of hydrolysis at room temperature. The lower the pH, the worse the solubility. The above two problems have greatly restricted the application of tea polyphenols and capryloyl glycine in functional skin care products, especially water-based products. Summary of the invention

[0007] In order to overcome the problems in the prior art that tea polyphenols are easily oxidized and capryloyl glycine has low solubility in water at room temperature, the present invention provides an oil-controlling and acne-removing tea polyphenol composition, a preparation method and an application thereof. The composition consists of tea polyphenols, capryloyl glycine and a solubilizing antioxidant. The solubilizing antioxidant can improve the solubility of capryloyl glycine in water at room temperature and significantly increase the content of capryloyl glycine in the aqueous solution. In addition, the solubilizing antioxidant can improve the antioxidant property of tea polyphenols and improve the stability of tea polyphenols. The composition is applied to aqueous cosmetics to significantly increase the oil-controlling and acne-removing effect and the product has high stability.

[0008] The specific technical scheme of the present invention is: An oil-controlling and acne-removing tea polyphenol composition comprises, by weight, 20 to 30 parts of tea polyphenols, 20 to 40 parts of capryloyl glycine, and 30 to 60 parts of a solubilizing antioxidant.

[0009] Preferably, the solubilizing antioxidant is one or more of sodium sulfite, sodium phytate, sodium citrate and sodium metabisulfite.

[0010] Preferably, the purity of the tea polyphenols is ≥80%, and the content of epigallocatechin gallate in the tea polyphenols is ≥30%.

[0011] The invention provides an oil-controlling and acne-removing tea polyphenol composition, which is composed of tea polyphenols, capryloyl glycine and a solubilizing antioxidant. The composition increases the room-temperature water solubility of capryloyl glycine by solubilizing the antioxidant, and significantly increases the content of capryloyl glycine in the aqueous solution. The solubilizing antioxidant can provide positively charged Na +Capryloylglycine is a derivative of glycine, which retains the chemical structure of α-hydroxyl, and the pKa value of α-hydroxyl is about 2.0. When pH>3.5, capryloylglycine is negatively charged and can form a supramolecular structure with the metal ions of the solubilizing antioxidant through electrostatic interaction, so that the room temperature water solubility of capryloylglycine is increased from 1.557g / L to more than 20g / L; in addition, the solubilizing antioxidant can be chelated by divalent or trivalent metal ions (such as Mg 2+ , Ca 2+ , Fe 3+ etc.), and as an inhibitor of polyphenol oxidase, inhibits the generation of free radicals, makes tea polyphenols less likely to undergo enzymatic oxidation and reddening, and avoids the browning of the phenolic hydroxyl groups in the tea polyphenol structure by metal ion catalysis and excessive oxidation of oxygen free radicals in aqueous solution. Therefore, the solubilizing antioxidant can also increase the stability of tea polyphenols and increase the antioxidant properties of tea polyphenols. The application of the composition to aqueous cosmetics can improve the stability of tea polyphenols, increase the content of capryloyl glycine, and improve the oil-controlling and acne-removing effects of aqueous products; in addition, the solubilizing antioxidant can improve the antioxidant properties of tea polyphenols and improve the stability of tea polyphenols. The application of the composition to aqueous cosmetics significantly increases the oil-controlling and acne-removing effects and the product has high stability.

[0012] A method for preparing the above-mentioned oil-controlling and acne-removing tea polyphenol composition comprises the following steps: placing a solubilizing antioxidant and tea polyphenols in water and mixing them until dissolved, then adding capryloyl glycine and continuing to stir until completely dissolved to prepare the oil-controlling and acne-removing tea polyphenol composition.

[0013] Preferably, the stirring temperature is 20-30°C.

[0014] Preferably, the stirring speed is 30-200 r / min.

[0015] An application of the above-mentioned oil-controlling and acne-removing tea polyphenol composition in water-based cosmetics comprises the following steps: adding auxiliary materials to the oil-controlling and acne-removing composition and stirring and mixing to form a water-based oil-controlling and acne-removing product.

[0016] Compared with the prior art, this application has the following technical effects: The oil-controlling and acne-removing tea polyphenol composition provided by the present invention consists of tea polyphenol, capryloyl glycine and a solubilizing antioxidant. The composition consists of tea polyphenol, capryloyl glycine and a solubilizing antioxidant. The composition makes the room-temperature water solubility of capryloyl glycine by the solubilizing antioxidant, thereby significantly increasing the content of capryloyl glycine in an aqueous solution. In addition, the solubilizing antioxidant can increase the antioxidant property of tea polyphenol and improve the stability of tea polyphenol. The composition is applied to aqueous cosmetics to significantly increase the oil-controlling and acne-removing effect and has high product stability. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a stability state diagram of Examples 1 to 6 of the present invention and Comparative Examples 1 to 8.

[0018] Figure 2 It is a bar chart showing the oil control efficacy of Example 1 and Comparative Examples 1 to 7 of the present invention. DETAILED DESCRIPTION

[0019] The present invention will be further described below in conjunction with the embodiments.

[0020] Embodiment 1: An oil-controlling and acne-removing tea polyphenol composition comprises, by mass, 20 parts of tea polyphenols, 30 parts of capryloyl glycine, and 50 parts of sodium sulfite.

[0021] A method for preparing the above-mentioned oil-controlling and acne-removing tea polyphenol composition comprises the following steps: Place 50 parts of sodium sulfite and 30 parts of capryloyl glycine in 20°C water, stir at 30 r / min until dissolved, then add 20 parts of tea polyphenols, continue stirring at 30 r / min until completely dissolved to prepare an oil-controlling and acne-removing tea polyphenol composition.

[0022] Embodiment 2: An oil-controlling and acne-removing tea polyphenol composition comprises, by mass, 30 parts of tea polyphenols, 40 parts of capryloyl glycine, and 30 parts of sodium sulfite.

[0023] A method for preparing the above-mentioned oil-controlling and acne-removing tea polyphenol composition comprises the following steps: 30 parts of sodium sulfite and 40 parts of capryloyl glycine were placed in 30°C water, stirred at 200 r / min until dissolved, and then 30 parts of tea polyphenols were added, and continued to stir at 200 r / min until completely dissolved to prepare an oil-controlling and acne-removing tea polyphenol composition.

[0024] Embodiment 3: An oil-controlling and acne-removing tea polyphenol composition comprises, by weight, 30 parts of tea polyphenols, 30 parts of capryloyl glycine, and solubilizing antioxidants including 3 parts of sodium pyrosulfite and 37 parts of sodium phytate.

[0025] A method for preparing the above-mentioned oil-controlling and acne-removing tea polyphenol composition comprises the following steps: 37 parts of sodium phytate, 3 parts of sodium metabisulfite and 30 parts of capryloyl glycine were placed in 25°C water, stirred at 100 r / min until dissolved, and then 30 parts of tea polyphenols were added, and stirring was continued at 100 r / min until completely dissolved to prepare an oil-controlling and acne-removing tea polyphenol composition.

[0026] Embodiment 4: An oil-controlling and acne-removing tea polyphenol composition comprises, by weight, 25 parts of tea polyphenols, 40 parts of capryloyl glycine, and solubilizing antioxidants including 5 parts of sodium sulfite, 2 parts of sodium pyrosulfite, and 28 parts of sodium citrate.

[0027] A method for preparing the above-mentioned oil-controlling and acne-removing tea polyphenol composition comprises the following steps: Place 5 parts of sodium sulfite, 2 parts of sodium metabisulfite, 28 parts of sodium citrate and 40 parts of capryloyl glycine in 28°C water, stir at 150 r / min until dissolved, then add 25 parts of tea polyphenols, continue stirring at 75 r / min until completely dissolved to prepare an oil-controlling and acne-removing tea polyphenol composition.

[0028] Embodiment 5: An oil-controlling and acne-removing tea polyphenol composition comprises, by weight, 25 parts of tea polyphenols, 35 parts of capryloyl glycine, and solubilizing antioxidants including 10 parts of sodium sulfite and 30 parts of sodium citrate.

[0029] A method for preparing the above-mentioned oil-controlling and acne-removing tea polyphenol composition comprises the following steps: Place 10 parts of sodium sulfite, 30 parts of sodium citrate and 35 parts of capryloyl glycine in 23°C water, stir at 50 r / min until dissolved, then add 25 parts of tea polyphenols, continue stirring at 125 r / min until completely dissolved to prepare an oil-controlling and acne-removing tea polyphenol composition.

[0030] An application of the above-mentioned oil-controlling and acne-removing tea polyphenol composition in aqueous cosmetics comprises the following steps: The raw materials of phase A (5.0wt% butanediol, 5.0wt% 1,2-pentanediol, 0.1wt% sodium hyaluronate, 0.5wt% p-hydroxyacetophenone and water) are mixed and heated to 80°C, stirred and completely dissolved, and the raw materials of phase B (0.2wt% dipotassium glycyrrhizinate, 1.0wt% panthenol) are added, stirred and dispersed until a uniform state, and then cooled to 40°C, and the raw materials of phase C (1.0wt% oil-controlling and acne-removing tea polyphenols composition) and phase D (0.5% 1,2-hexanediol, 0.1% PEG-60 hydrogenated castor oil and 0.02% flavor, the final product uses pH buffer arginine to adjust the pH to 5.0-5.5) are added, and stirred until completely dissolved to prepare a colorless and transparent toner.

[0031] Embodiment 6: An oil-controlling and acne-removing tea polyphenol composition comprises, by weight, 20 parts of tea polyphenols, 35 parts of capryloyl glycine, and solubilizing antioxidants including 5 parts of sodium pyrosulfite and 40 parts of sodium phytate.

[0032] A method for preparing the above-mentioned oil-controlling and acne-removing tea polyphenol composition comprises the following steps: Place 5 parts of sodium pyrosulfate, 40 parts of sodium phytate and 30 parts of capryloyl glycine in 28°C water, stir at 100 r / min until dissolved, then add 20 parts of tea polyphenols, continue stirring at 200 r / min until completely dissolved to prepare an oil-controlling and acne-removing tea polyphenol composition.

[0033] An application of the above-mentioned oil-controlling and acne-removing tea polyphenol composition in cream cosmetics comprises the following steps: The raw materials of phase A (5.0wt% butylene glycol, 5.0wt% 1,2-pentanediol, 0.2wt% dipotassium glycyrrhizate, 0.1wt% sodium hyaluronate, 0.3wt% acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, 0.5wt% p-hydroxyacetophenone and water) were stirred and dispersed completely, and heated to 80°C. The raw materials of phase B (3.0wt% camellia seed oil, 1.2wt% cetearyl alcohol, 1.0wt% glyceryl stearate citrate, 0.1%wt% tocopherol (vitamin E)) were heated to 75°C and dissolved completely. The raw materials of phase B were then transferred to the raw materials of phase A, and the mixture was fully homogenized and emulsified at 5000r / min to a uniform state, and then the temperature was lowered to 40°C. The raw materials of phase C (2.0wt% of the oil-controlling and acne-removing tea polyphenol composition) and the raw materials of phase C (0.5% 1,2-hexanediol, 0.1% PEG-60 hydrogenated castor oil and 0.02% flavor, the final product uses pH buffer arginine to adjust the pH to 5.0-6.0), and stir until completely dissolved to form a milky white emulsion.

[0034] Comparative Example 1: An oil-controlling and acne-removing tea polyphenol composition comprises 20 parts of tea polyphenols by mass.

[0035] A method for preparing the above-mentioned oil-controlling and acne-removing tea polyphenol composition comprises the following steps: 20 parts of tea polyphenols were placed in 20°C water and mixed at a stirring speed of 30 r / min until dissolved to prepare an oil-controlling and acne-removing tea polyphenol composition.

[0036] Comparative Example 2: An oil-controlling and acne-removing tea polyphenol composition comprises 20 parts of tea polyphenols and 30 parts of capryloyl glycine by mass.

[0037] A method for preparing the above-mentioned oil-controlling and acne-removing tea polyphenol composition comprises the following steps: 30 parts of capryloyl glycine were placed in 20°C water, and stirred at 30 r / min until dissolved. Then 20 parts of tea polyphenols were added, and stirring was continued at 30 r / min until completely dissolved to prepare an oil-controlling and acne-removing tea polyphenol composition.

[0038] Comparative Example 3 An oil-controlling and acne-removing tea polyphenol composition comprises 20 parts of tea polyphenols and 50 parts of sodium sulfite by mass.

[0039] A method for preparing the above-mentioned oil-controlling and acne-removing tea polyphenol composition comprises the following steps: Place 50 parts of sodium sulfite in 20°C water, stir at 30 r / min until dissolved, then add 20 parts of tea polyphenols, continue stirring at 30 r / min until completely dissolved to prepare an oil-controlling and acne-removing tea polyphenol composition.

[0040] Comparative Example 4: An oil-controlling and acne-removing tea polyphenol composition comprises, by weight, 20 parts of tea polyphenols, 30 parts of capryloyl glycine, and 50 parts of vitamin C.

[0041] A method for preparing the above-mentioned oil-controlling and acne-removing tea polyphenol composition comprises the following steps: Place 50 parts of vitamin C and 30 parts of capryloyl glycine in 20°C water, stir at 30 r / min until dissolved, then add 20 parts of tea polyphenols, continue stirring at 30 r / min until completely dissolved to prepare an oil-controlling and acne-removing tea polyphenol composition.

[0042] Comparative Example 5: An oil-controlling and acne-removing tea polyphenol composition comprises, by mass, 20 parts of tea polyphenols, 30 parts of capryloyl glycine, and 50 parts of ergothioneine.

[0043] A method for preparing the above-mentioned oil-controlling and acne-removing tea polyphenol composition comprises the following steps: 50 parts of ergothioneine and 30 parts of capryloylglycine were placed in 20°C water, stirred at 30 r / min until dissolved, and then 20 parts of tea polyphenols were added, and stirred at 30 r / min until completely dissolved to prepare an oil-controlling and acne-removing tea polyphenol composition.

[0044] Comparative Example 6: An oil-controlling and acne-removing tea polyphenol composition comprises, by mass, 20 parts of tea polyphenols, 30 parts of salicylic acid, and 50 parts of sodium sulfite.

[0045] A method for preparing the above-mentioned oil-controlling and acne-removing tea polyphenol composition comprises the following steps: Place 50 parts of sodium sulfite and 30 parts of salicylic acid in 20°C water, stir at 30 r / min until dissolved, then add 20 parts The tea polyphenols are stirred at 30 r / min until they are completely dissolved to prepare an oil-controlling and acne-removing tea polyphenol composition.

[0046] Comparative Example 7: An oil-controlling and acne-removing tea polyphenol composition comprises, by mass, 20 parts of tea polyphenols, 30 parts of capryloyl glycine, and 50 parts of arginine.

[0047] A method for preparing the above-mentioned oil-controlling and acne-removing tea polyphenol composition comprises the following steps: 50 parts of arginine and 30 parts of capryloyl glycine were mixed in 20°C water at a stirring speed of 30 r / min until dissolved, and then 20 parts of The tea polyphenols are stirred at 30 r / min until they are completely dissolved to prepare an oil-controlling and acne-removing tea polyphenol composition.

[0048] Comparative Example 8: An oil-controlling and acne-removing tea polyphenol composition comprises, by mass, 25 parts of tea polyphenols, 35 parts of capryloyl glycine, and 40 parts of sodium hydroxide.

[0049] A method for preparing the above-mentioned oil-controlling and acne-removing tea polyphenol composition comprises the following steps: 40 parts of sodium hydroxide and 35 parts of capryloyl glycine were mixed in 20°C water at a stirring speed of 30 r / min until dissolved, and then 25 parts of tea polyphenols were added, and stirring was continued at 30 r / min until completely dissolved to prepare an oil-controlling and acne-removing tea polyphenol composition.

[0050] An application of the above-mentioned oil-controlling and acne-removing tea polyphenol composition in aqueous cosmetics comprises the following steps: The raw materials of phase A (5.0wt% butanediol, 5.0wt% 1,2-pentanediol, 0.1wt% sodium hyaluronate, 0.5wt% p-hydroxyacetophenone and water) are mixed and heated to 80°C, stirred and completely dissolved, and the raw materials of phase B (0.2wt% dipotassium glycyrrhizinate, 1.0wt% panthenol) are added, stirred and dispersed until a uniform state, and then cooled to 40°C, and the raw materials of phase C (1.0wt% oil-controlling and acne-removing tea polyphenols composition) and phase D (0.5% 1,2-hexanediol, 0.1% PEG-60 hydrogenated castor oil and 0.02% flavor, the final product uses pH buffer arginine to adjust the pH to 5.0-5.5) are added, and stirred until completely dissolved to prepare a colorless and transparent toner.

[0051] Test example 1: The oil-controlling and acne-removing tea polyphenol compositions prepared in Examples 1-5 and Comparative Examples 1-7 were visually observed for sample status and placed under different conditions (25 degrees, 45 degrees, and 5 degrees) for 3 months to observe their stability. The test results are shown in Table 1. Table 1 Composition stability Example Tea polyphenols (parts) Capryloyl Glycine (part) Solubilizing antioxidant (parts) Composition stability Example 1 20 30 50 Soluble, transparent, stable color Example 2 30 40 30 Soluble, transparent, stable color Example 3 30 30 40 Soluble, transparent, stable color Example 4 25 40 35 Soluble, transparent, stable color Example 5 25 35 40 Soluble, transparent, stable color Example 6 20 35 45 Soluble, transparent, stable color Comparative Example 1 20 / / Can be dissolved to red and clear, fast color change Comparative Example 2 20 30 / Incomplete dissolution, fast color change Comparative Example 3 20 / 50 Can be dissolved to yellow and transparent, changing color quickly Comparative Example 4 20 30 50 Incomplete dissolution, the fastest color change Comparative Example 5 20 30 50 Incomplete dissolution, rapid color change, and more precipitation Comparative Example 6 20 30 50 Incomplete dissolution, with precipitation Comparative Example 7 20 30 50 Can be dissolved to light red and transparent, changing color quickly Comparative Example 8 25 35 40 Can be dissolved to light red and transparent, changing color quickly As shown in Table 1 and Figure 1As shown, the test results of samples in Examples 1 to 6 indicate that the composition containing a certain amount of tea polyphenols, capryloyl glycine and solubilizing antioxidants can effectively solve the application problems such as the instability of tea polyphenol raw materials and the low solubility of capryloyl glycine, while the composition without such a combination has problems such as poor sample solubility or stability. Comparative Example 1 contains only tea polyphenols, and the discoloration problem is serious; Comparative Example 2 contains tea polyphenols and capryloyl glycine, and the biggest problem is that the solubility is difficult to apply; Comparative Example 3 contains tea polyphenols and sodium sulfite, which can be completely dissolved, but because the pH of the composition is too high, it is not conducive to the stability of tea polyphenols; Comparative Example 4 contains the solubilizing antioxidant of the composition In comparative example 5, the solubilizing antioxidant was replaced by a commonly used antioxidant, vitamin C, but the composition still could not be completely dissolved and changed color faster; in comparative example 5, the solubilizing antioxidant was replaced by a commonly used antioxidant, ergothioneine, but the composition still could not be completely dissolved, and during the placement inspection, the insoluble precipitation increased; in comparative example 6, capryloyl glycine was replaced by salicylic acid, a commonly used acne-removing ingredient, but the composition was not completely dissolved and had precipitation; in comparative example 7, the solubilizing antioxidant was replaced by an inorganic base pH regulator arginine, and although the composition could be completely dissolved, it was easy to change color; in comparative example 8, the solubilizing antioxidant was replaced by an organic base pH regulator sodium hydroxide, and although the composition was completely dissolved, it was more prone to change color.

[0052] Test Example 2 The antibacterial property and oil control efficacy of the compositions prepared in Example 1 and Comparative Examples 1 to 7 were tested. Antibacterial property test steps: Antibacterial function study The antibacterial zone method was used to determine the sizes of the antibacterial zones of Example 1 and Comparative Examples 1 to 7 against the main pathogenic bacteria of acne, such as Propionibacterium acnes (P. acnes) and Staphylococcus aureus (S. aureus); the test results are shown in Table 2; Oil control efficacy test method: establish an oleic acid-stimulated human sebaceous gland cell SZ95 oil secretion model, set up a control group and a model group, and use Oil Red O staining to detect the inhibitory effect of Example 1 and Comparative Examples 1-7 on oleic acid-stimulated SZ95 cell lipid secretion. SZ95 cells were induced and stimulated with 100 μM oleic acid for 48 hours, and the induced SZ95 cells were collected and rinsed with PBS for 5 minutes, and then fixed with 10% formaldehyde for 10 minutes, washed with sterile water for 5 minutes, and washed with 60% isopropanol for 5 minutes, and then stained with Oil Red O dye for 15 to 20 minutes. The stained cell sections were placed under a microscope, the size of the lipid droplet distribution area of ​​the SZ95 cells was observed and filmed, and the lipid secretion of each group of SZ95 cells was qualitatively compared; the test results are shown in Figure 2 ; Table 2 Antibacterial test results Propionibacterium acnes inhibition zone (mm) Staphylococcus aureus (mm) Example 1 50.52 58.98 Comparative Example 1 10.75 13.85 Comparative Example 2 17.32 15.76 Comparative Example 3 20.33 17.95 Comparative Example 4 35.56 41.21 Comparative Example 5 24.54 33.51 Comparative Example 6 41.32 43.33 Comparative Example 7 20.54 25.90 As shown in Table 2, the inhibitory effects of different samples on Propionibacterium acnes are as shown in the table above, and the antibacterial effects are as follows: Example 1 > Comparative Example 6 > Comparative Example 4 > Comparative Example 7 > Comparative Example 5 > Comparative Example 3 > Comparative Example 2 > Comparative Example 1. The inhibitory effects of different samples on Staphylococcus aureus are as shown in the table above, and the antibacterial effects are as follows: Example 1 > Comparative Example 6 > Comparative Example 4 > Comparative Example 5 > Comparative Example 7 > Comparative Example 3 > Comparative Example 2 > Comparative Example 1.

[0053] like Figure 2 As shown, the study shows that the SZ95 cell nucleus in the control group is small. After stimulation with 100 μM oleic acid, the volume of SZ95 cells increases, the nucleus increases, and 100 μM oleic acid can promote the secretion of lipids in SZ95 cells. And the SZ95 cells were stained with Nile red dye. The fluorescence intensity of different sample groups was tested. The lower the fluorescence intensity, the stronger the effect of inhibiting the lipid secretion of SZ95 cells. Therefore, it can be seen from the above figure that the tested sample group can effectively inhibit the lipid secretion of SZ95 cells after oleic acid stimulation, and the inhibitory effects are as follows: Example 1>Comparative Example 6>Comparative Example 4>Comparative Example 7>Comparative Example 5>Comparative Example 3>Comparative Example 2>Comparative Example 1; It can be seen that Example 1 of the composition containing a certain amount of tea polyphenols, capryloyl glycine and solubilizing antioxidants, in the two tests of in vitro acne removal experiments, outperformed the composition containing only tea polyphenols (Comparative Example 1) and tea polyphenols and capryloyl glycine (Comparative Example 2) , tea polyphenols and solubilizing antioxidants (Comparative Example 3). At the same time, if the composition ingredients of the embodiment are replaced, whether it is replaced with the common acne-removing ingredient salicylic acid (Comparative Example 6), or replaced with the commonly used antioxidant vitamin C (Comparative Example 4), ergothioneine (Comparative Example 5), or replaced with the inorganic base arginine (Comparative Example 7), from the test data dimension, it is difficult to achieve the acne-removing effect of Example 1. Therefore, it can be explained that the tea polyphenols, capryloyl glycine and solubilizing antioxidants in the composition have synergistic synergy and enhance the effect of acne removal.

[0054] Test Example 3 The acceptance of the product efficacy description of the toners prepared in Example 5 and Comparative Example 8 was tested by using a consumer questionnaire survey and follow-up visit; The test steps include: recruiting >50 healthy subjects, and testing the oil control and acne removal effects of the toner made in Example 5 and Comparative Example 8 on the human body by retaining and using the product for 14 days. The test includes the following steps: Usage: After cleansing in the morning and evening, take an appropriate amount of oil control water and pour it on the palm of your hand, and evenly apply it to the facial skin. The amount can be increased in dry weather. Frequency of use: every morning and evening. Complete the self-assessment questionnaire before using the product, after using the product for 7 days, and after using the product for 14 days. The test samples are in the same packaging, marked as 5 for real and 8 for comparative, and distributed according to the random table. The product is used on half of the face; Subject inclusion criteria: Subjects are of both genders, preferably aged 13-18 years old, and in good health; Exclusion criteria for subjects: those who have used antihistamines in the past week or immunosuppressants or immunomodulatory related biological agents and small molecule drugs in the past month; those who have used any anti-inflammatory drugs in the test area in the past two months; subjects with inflammatory skin diseases that have not been cured clinically; insulin-dependent diabetes patients; those who have had an attack and treatment of allergic diseases or have used anti-inflammatory drugs such as hormones in the past month; those with serious systemic health problems such as cancer; patients with immunodeficiency or autoimmune diseases; women in the pregnancy preparation, lactation or pregnancy period; those whose skin test area has scars, pigmentation, atrophy, port wine stains or other blemishes that affect the determination of the test results; those who have participated in relevant clinical trials in the past three months; those with highly sensitive constitutions; non-voluntary participants or those who cannot complete the specified content according to the test requirements; other persons who are considered unsuitable to participate in the trial by the trial researchers; Product use effect research: A 5-point system is used, with 1 point representing strong opposition and 5 points representing very satisfied. The number of valid people with a score > 3 points is counted, and the calculation method for satisfaction is as follows: Recognition = (frequency of people who choose 3 points or above / total number of people) ╳ 100% The relevant efficacy can only be supported if the percentage of subjects agreeing (very satisfied and satisfied) is greater than 50%; the binomial test is used to calculate whether there is a significant difference between the probability of TOP2 (score>3 points) and BOT3 (score<3 points) and the specified test ratio, and the test ratio is 0.50; Product use results survey: 52 subjects' evaluation results on the product's efficacy were collected, and the statistical scores > 3 points were used to calculate the proportion of subjects who agreed with the efficacy description; the test results are shown in Table 3; Table 3 Subjects' recognition of product efficacy description Serial number Research Questions D7-Solid 5 D7-8 D14-Solid 5 D14-Pair 8 1 This product has moisturizing effect 100％ 96％ 100％ 93％ 2 This product has an instant oil control effect 100％ 90％ 100％ 85％ 3 This product has a long-lasting oil control effect 100％ 90％ 100％ 86％ 4 This product is suitable for sensitive skin 100％ 95％ 100％ 90％ 5 This product has a soothing effect 100％ 90％ 100％ 90％ 6 This product is mild and non-irritating 100％ 90％ 100％ 90％ As shown in Table 3, according to the consumer test results, the toner prepared in Example 5 is superior to the toner prepared in Comparative Example 8 in terms of instant and long-term oil control. At the same time, based on the moisturizing and soothing dimensions, more people choose the toner prepared in Example 5 than the toner prepared in Comparative Example 8.

[0055] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any way. Any simple modification, change and equivalent transformation made to the above embodiment based on the technical essence of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. An oil-controlling and acne-removing tea polyphenol composition, characterized in that: The composition comprises 20-30 parts of tea polyphenols, 30-40 parts of capryloyl glycine and 50-60 parts of solubilizing antioxidant by weight.

2. The oil-controlling and acne-removing tea polyphenol composition according to claim 1, characterized in that: The solubilizing antioxidant is one or more of sodium sulfite, sodium phytate, sodium citrate and sodium pyrosulfite.

3. The oil-controlling and acne-removing tea polyphenol composition according to claim 1, characterized in that: The purity of the tea polyphenols is ≥80%, and the content of epigallocatechin gallate in the tea polyphenols is ≥30%.

4. A method for preparing the oil-controlling and acne-removing tea polyphenol composition according to any one of claims 1 to 3, characterized in that: The following steps are involved: The solubilizing antioxidant and capryloyl glycine are placed in water and mixed until dissolved, and then tea polyphenols are added and stirred continuously until completely dissolved to prepare an oil-controlling and acne-removing tea polyphenol composition.

5. The preparation method according to claim 4, characterized in that: The stirring temperature is 20-30°C.

6. The preparation method according to claim 4 or 5, characterized in that: The stirring speed is 30-200 r / min.

7. Use of the oil-controlling and acne-removing tea polyphenol composition according to any one of claims 1 to 3 in cosmetics, characterized in that: The following steps are involved: The pre-dissolved aqueous solution of the oil-controlling and anti-acne composition and the auxiliary materials are stirred and mixed to prepare an oil-controlling and anti-acne product.

8. The use according to claim 7, characterized in that: The auxiliary materials include one or more of butylene glycol, 1,2-pentanediol, dipotassium glycyrrhizate, panthenol, sodium hyaluronate, acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, p-hydroxyacetophenone, camellia seed oil, cetearyl alcohol, glyceryl stearate citrate, tocopherol, 1,2-hexanediol, PEG-60 hydrogenated castor oil and essence.

9. The use according to claim 7, characterized in that: The oil-controlling and anti-acne product is one of an aqueous solution, an emulsion and a cream.

10. The use according to claim 9, characterized in that: The aqueous solution is toner.

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