Tea polyphenol detection method and tea polyphenol detection device based on infrared evanescent wave absorption spectrum

By employing infrared evanescent wave absorption spectroscopy based on a conical infrared fiber optic sensor, the problems of high cost, complex procedures, and water interference in tea polyphenol detection have been solved, enabling rapid, accurate, and sensitive tea polyphenol detection and significantly improving detection efficiency and accuracy.

CN121783903APending Publication Date: 2026-04-03WUHAN UNIV OF TECH
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Patent Information

Application Number
CN202511993435.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-26
Publication Date
2026-04-03

AI Technical Summary

Technical Problem

Existing tea polyphenol detection technologies suffer from problems such as high cost, complex detection process, susceptibility to interference, insufficient accuracy, long detection time, low efficiency, and low sensitivity. In particular, traditional infrared spectroscopy is affected by water interference, and conventional methods are easily affected by reducing substances in the sample.

Method used

Infrared evanescent absorption spectroscopy based on a conical infrared fiber optic sensor is employed. The conical infrared fiber optic sensor is encapsulated in the sample cell. Using an 850-4000 cm⁻¹ infrared light source and a micro-ring structure, infrared evanescent absorption spectra are collected. Quantitative analysis is performed using Lambert-Beer's law, and a standard curve for tea polyphenols is constructed to achieve rapid and accurate detection of tea polyphenols.

Benefits of technology

It achieves rapid, accurate, and sensitive detection of tea polyphenols, significantly reducing detection time and cost, avoiding interference from chemical reagents, and improving the specificity and stability of the detection. The sensitivity reaches 0.062 au/(mg·mL-1), and the detection limit is 0.25 mg/mL.

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Abstract

The invention belongs to the field of optical sensing technology and food component analysis, and discloses a tea polyphenol detection method and a tea polyphenol detection device based on an infrared evanescent wave absorption spectrum. Comprising the following steps that a conical infrared optical fiber sensor is provided, the conical infrared optical fiber sensor is obtained by drawing an optical fiber with the diameter of 150-1000 microns to obtain a conical infrared optical fiber, and then the end faces of the two ends of the conical infrared optical fiber sensor are polished till the end faces of the optical fiber are free of scratches, so that the two end faces of the optical fiber are flat, smooth and bright and form mirror faces; the conical infrared optical fiber sensor is packaged in the sample pool, the upper end of the sample pool is open, and a to-be-detected sample solution is injected and fully coats the optical fiber; an 850-4000 cm <-1 > infrared light source is provided, focused infrared light enters the optical fiber and is fully coupled with the optical fiber, an infrared evanescent wave absorption spectrum is collected, and qualitative and quantitative analysis is carried out on the tea polyphenol content based on the tea polyphenol characteristic peak intensity. The method for detecting the tea polyphenol has the characteristics of rapidness, high sensitivity and low detection limit.
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