Sunscreen product taking tripeptide-1 and hydrolyzed wheat protein as active ingredients and preparation method thereof

Abstract

The invention provides a sunscreen product with tripeptide-1 and hydrolyzed wheat protein as active ingredients and a preparation method of the sunscreen product. Comprising a phase A, a phase D, a phase B and a phase C, wherein the phase A and the phase D synergistically generate a sunscreen effect; the phase B is used for after-sun repair; wherein the phase A comprises Arabic gum, hydrolyzed wheat protein, squalane, tremella polysaccharide, L-arginine hydrochloride, tromethamine, a preservative and deionized water; the phase B comprises gellan gum; the phase C is prepared from glycerol, polyglycerol-3 methyl glucose distearate, small molecular hyaluronic acid and nicotinamide; and the phase D comprises a centella asiatica extract, tripeptide-1 and a rhodiola rosea extract. The phase A, the phase B, the phase C and the phase D are designed and combined into a multi-dimensional skin repair system, the dual effects of sunscreen and repair are achieved, the decorous feeling and white residues of a physical sunscreen agent can be avoided, the irritation of a chemical sunscreen agent to the skin and the attenuation of the sunscreen effect can also be avoided, and the light and thin base liquid and repair components are adopted, so that the skin repair liquid is more suitable for sensitive skin to use.

Description

Technical Field

[0001] The invention belongs to the technical field of sunscreen products, and particularly relates to a sunscreen product with tripeptide-1 and hydrolyzed wheat protein as active ingredients and a preparation method thereof. Background Art

[0002] At present, sunscreen products on the market are mainly based on three modes: physical sunscreen, chemical sunscreen and physical and chemical combined sunscreen. The basic formula ingredients and working principles include:

[0003] (1) Physical sunscreen products, the main ingredients include inorganic particles such as titanium dioxide (TiO2) and zinc oxide (ZnO); the formula usually also includes emulsifiers and water / oil phase bases. They are in the form of creams or lotions. The sunscreen principle is: through the high reflectivity of the particle surface, ultraviolet rays are directly reflected or scattered to prevent them from entering the skin.

[0004] However, due to the large particle size, strong coverage and heavy texture of sunscreen particles, it is easy to form obvious white residue (whitening phenomenon) on the skin surface. In addition, since physical sunscreen particles are not easily absorbed by the skin, they are relatively stable and have a long-lasting sunscreen effect. However, sunscreen products have poor air permeability and are prone to cause acne or greasy feeling.

[0005] (2) Chemical sunscreen products, the main ingredients include organic sunscreens such as avobenzone, benzophenone, and octyl methoxycinnamate (OMC); the formula is usually combined with solubilizers, antioxidants, and skin barrier repair ingredients. They are available in the form of lotions, gels, sprays, etc. The sunscreen principle is: after chemical sunscreens absorb ultraviolet rays, they convert ultraviolet energy into heat energy through intramolecular electron transitions, and then release it, thereby reducing the damage of ultraviolet rays to the skin.

[0006] However, due to the need for molecular structure stability, some chemical sunscreens will degrade and become ineffective under long-term ultraviolet radiation. Therefore, stabilizers (such as Tinosorb) are usually added to increase the durability of sunscreen. In addition, chemical sunscreen products are thin, transparent, and not easy to turn white, but the sunscreen effect will decay over time and need to be reapplied frequently.

[0007] (3) Physical and chemical combined sunscreen products mainly combine physical sunscreens (TiO2, ZnO) + chemical sunscreens (OMC, Avobenzone, etc.) to achieve a broad-spectrum protection effect. Generally, a lotion or cream system is used, combined with antioxidants and moisturizers to optimize skin feel. Its sunscreen principles include: providing immediate protection through physical sunscreens to reflect ultraviolet rays; providing long-term absorption protection through chemical sunscreens, and reducing the whitening problem caused by physical sunscreen. However, physical and chemical combined sunscreen products usually follow the mechanism of action of physical and chemical sunscreen products, and it is difficult to strike a balance between skin feel experience and sunscreen durability.

[0008] In summary, how to obtain a sunscreen skin care product that takes into account both skin feel and sunscreen performance through sunscreen mechanism and formula design is a technical problem that needs to be urgently solved by technical personnel in this field. Summary of the invention

[0009] The purpose of the present invention is to provide a sunscreen product with tripeptide-1 and hydrolyzed wheat protein as active ingredients. The present invention breaks through the limitations of traditional physical and chemical sunscreen products and proposes a high-efficiency sunscreen product based on the synergistic effect of tripeptide-1 and hydrolyzed wheat protein. It can not only balance the excellent sunscreen effect, durability and skin-friendly experience, but also has a skin repair effect, and can form a synergistic barrier in the stratum corneum and dermis of the skin, further improving the sunscreen and skin repair capabilities.

[0010] To achieve the above object, the present invention provides a sunscreen product with tripeptide-1 and hydrolyzed wheat protein as active ingredients, the sunscreen product comprising phase A and phase D that synergistically produce sunscreen effects, phase B for after-sun repair, and phase C as a light base liquid;

[0011] Among them, the phase A includes gum arabic, hydrolyzed wheat protein, squalane, tremella polysaccharide, L-arginine hydrochloride, tromethamine, a preservative and deionized water; the phase B includes gellan gum; the phase C includes glycerin, polyglycerol-3 methylglucose distearate, small molecule hyaluronic acid and niacinamide; the phase D includes Centella asiatica extract, tripeptide-1 and Rhodiola rosea extract.

[0012] In a preferred embodiment, the mass ratio of phase A, phase B, phase C and phase D is 100:(0.53-0.65):(10.53-12.87):(5.32-6.50); ​​preferably, the mass ratio of phase A, phase B, phase C and phase D is 100:(0.56-0.62):(11.12-12.29):(5.61-6.21); more preferably, the mass ratio of phase A, phase B, phase C and phase D is 100:0.59:11.70:5.91.

[0013] In a preferred embodiment, per 100 parts of the phase A, the following components are included, by weight: 82.42-91.10 parts of deionized water, 0.34-0.37 parts of gum arabic, 2.25-2.48 parts of hydrolyzed wheat protein, 3.93-4.34 parts of squalane, 5.61-6.21 parts of Tremella polysaccharide, 0.05-0.07 parts of L-arginine hydrochloride, 0.05-0.07 parts of tromethamine and 0.34-0.37 parts of preservatives;

[0014] Preferably, every 100 parts of phase A include the following components: 86.76 parts of deionized water, 0.35 parts of gum arabic, 2.36 parts of hydrolyzed wheat protein, 4.14 parts of squalane, 5.91 parts of tremella polysaccharide, 0.06 parts of L-arginine hydrochloride, 0.06 parts of tromethamine and 0.35 parts of a preservative; more preferably, the preservative includes sodium benzoate and / or potassium sorbate; most preferably, the preservative consists of sodium benzoate and potassium sorbate in a mass ratio of 2:1.

[0015] In a preferred embodiment, per 100 parts of the phase D, the following components are included: 15-22 parts of Centella asiatica extract, 18-40 parts of tripeptide-1 and 50-60 parts of Rhodiola rosea extract;

[0016] Preferably, every 100 parts of the phase D comprises the following components: 20-16.77 parts of Centella asiatica extract, 0-33.33 parts of tripeptide-12 and 50-60 parts of Rhodiola rosea extract.

[0017] In a preferred embodiment, in the phase C, the mass ratio of glycerol, polyglyceryl-3 methylglucose distearate, small molecule hyaluronic acid and niacinamide is 3.5:(0.2-0.6):(0.5-1.5):(2-8);

[0018] Preferably, in the phase C, the mass ratio of glycerol, polyglyceryl-3 methylglucose distearate, small molecule hyaluronic acid and niacinamide is 3.5:0.4:1:5.

[0019] In a preferred embodiment, the SPF value of the sunscreen product can reach 48±2, the PA value can reach PA++++, and the photostability residual activity can reach 91.1% after 8 hours of simulated sun exposure.

[0020] In a preferred embodiment, the HaCaT cell survival rate of the sunscreen product can reach 88.3±2.1%, the IL-6 reduction rate can reach 61.4%, and the DPPH free radical scavenging rate can reach 89.2%.

[0021] Another object of the present invention is to provide a method for preparing a sunscreen product with tripeptide-1 and hydrolyzed wheat protein as active ingredients, wherein phase A, phase B and phase C are prepared separately, mixed evenly, and then phase D ingredients are added and mixed evenly. The preparation method is simple and efficient, and the preparation method does not affect the activity of the raw materials, and can also enhance the sunscreen effect synergistically produced by tripeptide-1 and hydrolyzed wheat protein.

[0022] To achieve the above object, the present invention provides a method for preparing a sunscreen product with tripeptide-1 and hydrolyzed wheat protein as active ingredients, which specifically comprises the following steps:

[0023] The components of phase A and phase C are mixed according to a mass ratio to prepare phase A and phase C;

[0024] Dissolve phase B with deionized water to form a uniform gellan gum solution;

[0025] Slowly add the obtained gellan gum solution into phase A, and stir to obtain a uniform mixture of phase A and phase B; preferably, the stirring conditions in this step include: stirring speed 80-100 rpm, stirring time 5-8 min;

[0026] Slowly add phase C to the obtained mixture of phase A and phase B, and stir until the phases are evenly mixed; preferably, the stirring conditions in this step include: stirring speed 100-120 rpm, stirring time 10-15 min;

[0027] Add the Centella asiatica extract, tripeptide-1 and rhodiola rosea extract in the phase D components to the system in sequence, stir evenly, and obtain; to ensure sufficient mixing, in this step, after adding each component, continue stirring at 150-180 rpm for 2-3 minutes before adding the next component.

[0028] In a preferred embodiment, the preparation of phase A comprises the following steps:

[0029] Slowly add gum arabic into the stirred deionized water and stir until it is evenly dispersed; preferably, the stirring speed of the stirred deionized water is 30-50 rpm;

[0030] Slowly add hydrolyzed wheat protein, stir until evenly mixed, then drop squalane, stir until completely emulsified to form a uniform emulsion; preferably, the stirring speed after adding the hydrolyzed wheat protein is 100-120 rpm, and the stirring time is 3-6 minutes; the stirring speed when dropping squalane is 30-50 rpm, and the stirring time is 8-12 minutes;

[0031] Add Tremella polysaccharide to the emulsion, stir until the Tremella polysaccharide is fully dissolved, then add L-arginine hydrochloride, tromethamine and preservative in sequence, stir evenly, and obtain. Preferably, after adding Tremella polysaccharide, the stirring speed is 150-180rpm, and the stirring time is 8-10min; to ensure full mixing, after adding each component of L-arginine hydrochloride, tromethamine and preservative in sequence, continue stirring at 150-180rpm for 1-2min before adding the next component.

[0032] In a preferred embodiment, in the gellan gum solution, the mass ratio of phase B to deionized water is 1:(100-200).

[0033] In a preferred embodiment, the preparation of phase C comprises the following steps:

[0034] Mix glycerin, polyglyceryl-3 methyl glucose distearate, small molecule hyaluronic acid and niacinamide, and stir evenly to obtain the product.

[0035] Compared with the prior art, the technical solution of the present invention has the following advantages:

[0036] 1. In the present invention, tripeptide-1 is used as a signal peptide to activate skin cells and improve the skin barrier function. It can also promote collagen synthesis and improve the skin's defense ability. Hydrolyzed wheat protein can combine with the skin's natural barrier because it is rich in short peptides and amino acid chains. It also has good adsorption and skin-friendliness, which can enhance the skin's retention of active ingredients and improve durability. The present invention combines tripeptide-1 and hydrolyzed wheat protein, and creatively discovers the synergistic effect of the two on sunscreen performance. Through peptide-protein interaction, the skin barrier function is enhanced in the form of signal transmission and antioxidant synergism, which can produce anti-ultraviolet performance and reduce UV damage. Combined with other components, it effectively improves the stability and durability of sunscreen and reduces the need for re-application.

[0037] 2. The technical solution provided by the present invention does not add traditional physical sunscreen ingredients (inorganic powders such as titanium dioxide and zinc oxide) and chemical sunscreen ingredients (organic sunscreens such as avobenzone, benzophenone, and octyl methoxycinnamate). Therefore, compared with traditional sunscreens, this solution avoids the heavy feeling and white residue of physical sunscreen ingredients, and can also avoid the irritation of chemical sunscreen ingredients to the skin and the problem of attenuation of sunscreen effect. In addition, the sunscreen product of this solution has after-sun repair and skin care plant ingredients, which is more suitable for sensitive skin.

[0038] 3. The present invention combines a "multi-dimensional skin repair system" by designing phase A, phase B, phase C and phase D to achieve the dual effects of sun protection + repair, and all-round protection from UV protection to post-sun repair. Specifically including Centella Asiatica Extract and Rhodiola Extract, which have anti-inflammatory and soothing effects. Used in conjunction with tripeptide-1 and hydrolyzed wheat protein, it can reduce the redness and inflammation caused by ultraviolet rays, promote cell repair, and prevent post-sun pigmentation and skin sensitivity. Tremella Fuciformis Polysaccharide and Squalane provide a double moisturizing barrier to enhance skin hydration. Used in conjunction with tripeptide-1 and hydrolyzed wheat protein, the sunscreen effect can be more fitted to the skin and not easy to fall off. Niacinamide can reduce melanin deposition, prevent photoaging, and improve skin brightness. Low Molecular Weight Hyaluronic Acid can deeply hydrate the skin, increase skin moisture content, enhance repair ability, and reduce dryness.

[0039] 4. The present invention adopts a light and thin base liquid to avoid the heavy skin feel of traditional sunscreen products, and it is light and breathable for daily use. In the present invention, glycerin (Glycerin) + polyglyceryl-3 methylglucose distearate (Polyglyceryl-3Methylglucose Distearate) can make the various ingredients of different properties in the sunscreen product evenly distributed, avoiding the heavy, white and greasy problems brought by traditional sunscreens. In addition, the emulsified stabilization system in phase A makes the skin more breathable and acne-free. At the same time, due to the synergistic effect of tripeptide-1 and hydrolyzed wheat protein, high-efficiency sunscreen can be maintained under the premise of the most streamlined sunscreen ingredients, thereby reducing skin irritation and reducing the burden on the skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] These and / or other aspects and advantages of the present invention will become more clear and easier to understand from the following detailed description of the embodiments of the present invention in conjunction with the accompanying drawings, in which:

[0041] Figure 1 The protective effect of the sunscreen product prepared in Example 1 of the present invention against ultraviolet rays when applied;

[0042] Figure 2 This is the protective effect of the sunscreen product prepared in Example 1 of the present invention against ultraviolet rays after being left in the sun for 8 hours. DETAILED DESCRIPTION

[0043] In order to enable those skilled in the art to better understand the present invention, the present invention is further described in detail below in conjunction with the accompanying drawings and specific implementation methods, but it should be understood that the protection scope of the present invention is not limited to the specific implementation methods.

[0044] The embodiments of the present invention provide a sunscreen product with tripeptide-1 and hydrolyzed wheat protein as active ingredients and a preparation method thereof, thereby effectively solving the problems in the prior art of the heavy feeling and white residue of traditional physical sunscreen ingredients, the irritation of chemical sunscreen ingredients to the skin and the attenuation of the sunscreen effect, and the difficulty of physical and chemical combined sunscreen products in balancing the skin feel experience and sunscreen durability.

[0045] The technical solution of the present application is described in detail below through specific embodiments:

[0046] If not specifically indicated, the technical means used in the present invention are conventional means well known to those skilled in the art, and the various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods. The reagents used in the present invention are analytically pure unless otherwise specified. The slow addition of the present invention is 2 mL / min if there is no special instruction. The commercially available chemical sunscreen product used in the embodiments of the present invention is La Roche-Posay sunscreen, and the commercially available physical sunscreen product is Thinkbaby physical sunscreen.

[0047] In the present invention, parts by weight may be μg, mg, g, kg or other weight units known in the art, or multiples thereof, such as 1 / 10, 1 / 100, 10 times, 100 times or the like.

[0048] Example 1

[0049] A sunscreen product with tripeptide-1 and hydrolyzed wheat protein as active ingredients is prepared by the following method:

[0050] (i) Prepare phase A, phase B and phase C separately:

[0051] Preparation of Phase A:

[0052] 1. Add 73.4 parts by weight of deionized water into a stirring container, turn on the stirrer, set the stirring speed to low (30 rpm), keep the water in a slightly flowing state, and avoid generating bubbles.

[0053] 2. Take 0.3 parts by weight of gum arabic, divide it into small portions, and slowly add it to the stirring container. Stir for 2 minutes after each addition. Add the next portion after the gum arabic is completely dispersed, and gradually increase the stirring speed to 100 rpm until the gum arabic is completely dissolved and the solution is uniform.

[0054] 3. Slowly add 2 parts by weight of hydrolyzed wheat protein, keep the stirring speed at 100 rpm, and stir for 5 minutes to ensure that the hydrolyzed wheat protein is fully mixed.

[0055] 4. Use a dropper or other tool to slowly add 3.5 parts by weight of squalane, while reducing the stirring speed to 30 rpm, and continue stirring for 10 minutes until the squalane is completely emulsified to form a uniform emulsion.

[0056] 5. Slowly add 5 parts by weight of Tremella polysaccharide, increase the stirring speed to 150 rpm, and stir for 8 minutes to fully dissolve the Tremella polysaccharide.

[0057] 6. Add 0.05 parts by weight of L-arginine hydrochloride, 0.05 parts by weight of tromethamine, 0.2 parts by weight of sodium benzoate and 0.1 parts by weight of potassium sorbate in sequence, stirring for 1 minute after adding each ingredient to ensure that it is fully mixed and set aside.

[0058] Prepare phase B solution: Mix gellan gum and deionized water in a mass ratio of 1:146.8 and dissolve into a uniform gellan gum solution, taking care to avoid agglomeration, and set aside.

[0059] Prepare phase C: In another container, mix 3.5 parts by weight of glycerin, 0.4 parts by weight of polyglyceryl-3 methylglucose distearate, 1 part by weight of small molecule hyaluronic acid and 5 parts by weight of niacinamide, stir gently with a stirring rod to ensure that all ingredients are fully mixed, and set aside.

[0060] (ii) Mixing phase A, phase B and phase C:

[0061] The gellan gum solution prepared in phase B was slowly added to phase A while maintaining the stirring speed at 80 rpm for 5 minutes to ensure that the gellan gum was evenly dispersed in phase A to obtain a stable mixture of phases A and B.

[0062] Slowly add phase C to the mixture of phase A and phase B, continue stirring for 10 minutes, and adjust the stirring speed to 100 rpm to ensure that the phases are fully mixed.

[0063] (III) Adding phase D components into the system:

[0064] In the mixture of phase A, phase B and phase C, add 1 part by weight of Centella asiatica extract, 1 part by weight of tripeptide-1 and 3 parts by weight of Rhodiola rosea extract in sequence, stirring for 2 minutes after each addition to ensure uniform mixing, and you will get a sunscreen product that takes into account both skin feel and sun protection performance.

[0065] Example 2

[0066] Compared with Example 1, the only difference of this example is that the amount of Centella asiatica extract is reduced from 1 weight part to 0.5 weight part, the amount of Rhodiola rosea extract is reduced from 3 weight parts to 1.5 weight parts, and the weight part of deionized water is increased from 73.4 weight parts to 75.4 weight parts. The other raw materials and methods are exactly the same as those in Example 1.

[0067] Comparative Example 1

[0068] Compared with Example 1, the only difference of this comparative example is that no hydrolyzed wheat protein is added, and its amount is proportionally distributed to other components in phase A except water and preservatives. The other raw materials and methods are exactly the same as those in Example 1.

[0069] That is, phase A of Comparative Example 1 includes 73.4 parts by weight of deionized water, 0.37 parts by weight of gum arabic, 4.29 parts by weight of squalane, 6.12 parts by weight of Tremella polysaccharide, 0.06 parts by weight of L-arginine hydrochloride, and 0.06 parts by weight of tromethamine.

[0070] Comparative Example 2

[0071] Compared with Example 1, the only difference of this comparative example is that tripeptide-1 is not added, and its amount is proportionally distributed to other components in phase D. The other raw materials and methods are exactly the same as those in Example 1.

[0072] That is, in phase D of Comparative Example 2, 1.25 parts by weight of Centella asiatica extract and 3.75 parts by weight of Rhodiola rosea extract were included.

[0073] Comparative Example 3

[0074] Compared with Example 1, the difference of this comparative example is only that the amount of hydrolyzed wheat protein is reduced from 2 parts by weight to 1 part by weight, and the weight of deionized water is increased from 73.4 parts by weight to 74.4 parts by weight. The other raw materials and methods are exactly the same as those in Example 1.

[0075] Comparative Example 4

[0076] Compared with Example 1, the difference of this comparative example is that the amount of tripeptide-1 is reduced from 1 weight part to 0.5 weight part, and the weight part of deionized water is increased from 73.4 weight parts to 73.9 weight parts. The other raw materials and methods are exactly the same as those in Example 1.

[0077] Comparative Example 5

[0078] Compared with Example 1, the difference of this comparative example is only that: the amount of tripeptide-1 is reduced from 1 weight part to 0.5 weight part, the amount of hydrolyzed wheat protein is reduced from 2 weight parts to 1 weight part, and the weight part of deionized water is increased from 73.4 weight parts to 74.9 weight parts, and the other raw materials and methods are exactly the same as those in Example 1.

[0079] Application Example 1

[0080] The SPF, PA value, UVA absorbance, UVB absorbance and light stability of Examples 1-2 of the present invention, Comparative Examples 1-5 and commercially available traditional sunscreen products were tested for effectiveness. The test methods and results are as follows:

[0081] SPF value: In accordance with the "Method for Determination of Sun Protection Index (SPF) of Sunscreen Cosmetics" in the "Technical Specifications for Safety of Cosmetics" (2015 edition), the SPF value is calculated through human skin testing by comparing the minimum erythema dose of skin using sunscreen products and not using products.

[0082] PA value: According to the "Determination Method of Long-wave Ultraviolet Protection Index (PFA Value) of Sunscreen Cosmetics" in the "Technical Specifications for Safety of Cosmetics", the protection effect of the sample against long-wave ultraviolet rays (UVA) is measured, the PFA value is calculated and converted into a PA grade (such as PFA value ≥ 32 is marked as PA++++). UVA absorbance, UVB absorbance: Prepare the sunscreen product solution, use an ultraviolet spectrophotometer, measure the absorbance in the UVA band (320-400nm) and UVB band (280-320nm), and calculate the absorbance according to the formula: Absorbance (%) = (1-(transmittance / transmittance of blank control group)) × 100%

[0083] At the same time, Example 1 is applied to the upper left corner of the UV sensor card. The sunscreen effect during application is as follows: Figure 1 After being placed outdoors in the sun for 8 hours, the UV protection effect is as follows: Figure 2 shown.

[0084] Photostability (% Remaining Activity): 2 hours UV exposure test using ISO 24443 in vitro method commonly used for rapid screening of photostability of formulations, including:

[0085] According to the requirements of the "Technical Specifications for Safety of Cosmetics", apply the sunscreen product to the test carrier, and after continuous ultraviolet irradiation for 2 hours, measure the SPF value again and calculate the remaining activity: photostability (% remaining activity) = (SPF value after ultraviolet irradiation / SPF value before ultraviolet irradiation) × 100%

[0086] The test results are shown in Table 1.

[0087] Table 1

[0088]

[0089] As can be seen from Table 1, the test data of the sunscreen products of Examples 1-2 are significantly better than those of physical and chemical sunscreen products on the market, indicating that the sunscreen products provided by the present invention have practical effects in terms of sunscreen performance. However, the sunscreen effects of Comparative Examples 1-2, which do not add hydrolyzed wheat protein or tripeptide-1, and Comparative Examples 3-5, in which the amount of hydrolyzed wheat protein or tripeptide-1 is significantly reduced, are significantly weaker than those of the examples and the commercially available products, verifying that the tripeptide-1 and the hydrolyzed wheat protein in the present invention produce a synergistic effect in terms of sunscreen.

[0090] Application Example 2

[0091] The skin repairing ability of Examples 1-2 of the present invention, Comparative Examples 1-5 and commercially available traditional sunscreen products was verified. The specific test methods and results are as follows:

[0092] 1. HaCaT cell survival rate

[0093] Methods: MTT method was used. HaCaT cells were seeded in 96-well plates at an appropriate density. After 24 hours of culture, different formulas of sunscreen products were added (the control group was added with the same amount of culture medium) and cultured for another 24 hours. MTT solution was added to each well, and after 4 hours of incubation, the supernatant was discarded, DMSO was added to dissolve the formazan crystals, and the absorbance (OD value) at 570nm was measured with a microplate reader.

[0094] Calculation formula: Cell survival rate (%) = OD value of experimental group / OD value of control group × 100%

[0095] 2. IL-6 reduction rate

[0096] Methods: ELISA was used to detect. HaCaT cells were first induced with lipopolysaccharide (LPS) to construct an inflammatory model, and then sunscreen products were added. After a certain period of culture, the cell supernatant was collected. The IL-6 content in the supernatant was determined according to the IL-6 ELISA kit steps.

[0097] Calculation formula: IL-6 reduction rate (%) = (IL-6 content in control group - IL-6 content in experimental group) / IL-6 content in control group × 100%

[0098] 3. DPPH free radical scavenging rate

[0099] Method: Spectrophotometry was used. Sunscreen products were mixed with DPPH ethanol solution in proportion, and reacted for 30 minutes in the dark. Anhydrous ethanol was used as a blank control, and the absorbance (A) at 517 nm was measured.

[0100] Calculation formula: DPPH clearance rate = [(A blank - A sample) / A blank] × 100%, where A blank is the absorbance of the DPPH solution when no sample is added, and A sample is the absorbance of the DPPH solution after adding the sample to be tested.

[0101] The test results are shown in Table 2.

[0102] Table 2

[0103]

[0104] It can be seen from Table 2 that commercially available chemical or physical sunscreen products have certain damage to the skin, while the sunscreen products provided by Examples 1-2 of the present invention have obvious protective effects on the skin, achieving the dual effects of sunscreen + repair. At the same time, it can also be seen from Comparative Examples 1-2 that the use of tripeptide-1 or hydrolyzed wheat alone cannot achieve excellent protective effects, indicating that tripeptide-1 and hydrolyzed wheat have a synergistic effect in resisting ultraviolet rays and reducing UV damage.

[0105] Application Example 3

[0106] The skin feel and air permeability of Examples 1-2 of the present invention, Comparative Examples 1-5 and commercially available traditional sunscreen products were tested for effectiveness. The specific test methods and results are as follows:

[0107] 1. Changes in breathability

[0108] Methods: Forty healthy volunteers aged 18-45 years (20 males and 20 females) were selected and the skin air permeability tester (Courage+Khazaka TM), the test environment temperature was 25±1℃, humidity was 60±5%, the same part of the inner forearm of the subjects was selected, and the skin gas exchange rate was measured before and 1 hour after the application of the sample. The data were expressed as mean ± standard deviation, and the CV value was ≤10%.

[0109] Calculation formula: Air permeability retention rate (%) = gas exchange rate after application / gas exchange rate before application × 100%. The higher the retention rate (closer to 100%), the smaller the impact of the product on skin permeability.

[0110] 2. Skin oiliness and skin moisture and oil content

[0111] Methods: 40 healthy volunteers aged 18-45 years (20 males and 20 females) were randomly divided into 8 groups, 5 in each group. After cleaning their faces every morning, volunteers in each group evenly applied sunscreen products on the designated parts (cheeks) and recorded the greasy feeling of the facial skin at the time of application. Among them, 1 point (no greasy feeling); 2 points (slightly greasy); 3 points (relatively greasy); 4 points (1 obviously greasy); 5 points (very greasy). Use for 5 consecutive days and take the average value of the measurement and record it.

[0112] At the same time, use a skin moisture tester and a skin oil tester to test the moisture and oil content of the cheek skin at a specific time. Use it for 5 consecutive days and record the average value of the measurement.

[0113] 3. Skin moisture content improvement rate

[0114] Calculation formula: Skin moisture content increase (%) = (moisture content after use - moisture content before use) / moisture content before use × 100%

[0115] 4. Skin feel rating (10 points)

[0116] Methods: After a full 28-day test period, the above 40 volunteers were asked to make subjective ratings based on dimensions such as "smoothness of use, skin-fitting feel, and foreign body sensation", and the average score was calculated based on a 10-point system (10 points for the best and 1 point for the worst).

[0117] The test results are shown in Table 3 and Table 4.

[0118] formula Air permeability retention rate (%) Grease (1-5) Skin feel rating (10 points) Example 1 85.6 1.3 9.1 Example 2 80.2 1.5 8.5 Comparative Example 1 78.0 1.8 8.2 Comparative Example 2 76.0 2.0 8.0 Comparative Example 3 75.3 2.0 8.0 Comparative Example 4 70.1 2.5 7.5 Comparative Example 5 60.0 3.0 7.0 Commercially available chemical sunscreen products 68.2 3.2 6.8 City shopping sunscreen products 52.3 4.1 5.4

[0119] Table 4

[0120]

[0121]

[0122]

[0123] As can be seen from Table 3 and Table 4, the water content of the sunscreen products prepared in Examples 1-2 of the present invention is significantly increased after use, and still maintains a high level after 8 hours; at the same time, the oil content is always well controlled, reflecting the optimization of water-oil balance. In terms of air permeability and skin feel, the sunscreen products of Examples 1-2 are also significantly improved compared to the products of Comparative Examples 1-5, so that consumers have a good experience when applying on the face. Compared with commercially available sunscreen products, although the commercially available physical sunscreen products are close to some of the index data of this embodiment in terms of sunscreen performance in Table 1, from the effects of Tables 3 and 4, the oil content of physical sunscreen products increases more significantly after use, and the water content maintenance is weak, reflecting its heavy skin feel and insufficient ability to regulate water-oil balance. Commercially available chemical sunscreen products are also significantly inferior to the products of the present invention in terms of hydration and oil control, and their water-oil index drops significantly after 8 hours.

[0124] Application Example 4

[0125] The sunscreen stability and durability of Example 1 of the present invention and commercially available traditional sunscreen products were verified by using the ISO 24444 human body method, which is closer to the actual use scenario, to simulate sun exposure, specifically including:

[0126] According to the requirements of the "Technical Specifications for Safety of Cosmetics", the sunscreen product was applied to the face of the test subject, and after standing outdoors in the sun for N hours, the SPF value was measured again, and the residual activity was calculated: photostability (% residual activity) = (SPF value after simulated sun exposure / SPF value before simulated sun exposure) × 100%

[0127] This application example uses a simulated sun exposure method, which is different from the continuous ultraviolet irradiation conditions in Application Example 1. Simulated sun exposure is closer to the actual daily use environment, and the SPF value decays more significantly, which can further illustrate the stability and durability of sunscreen products.

[0128] The test results are shown in Table 5.

[0129] Table 5

[0130]

[0131] As can be seen from Table 5, after 8 hours of simulated sun exposure, the SPF value of the sunscreen product prepared by the present invention is still maintained at 41±3, and the residual activity exceeds 90%, indicating that the sunscreen effect is stable; while the SPF value of the commercially available chemical sunscreen drops significantly after 8 hours of simulated sun exposure, and the residual activity is less than 60%. It can be seen that the formula containing synergistic ingredients of the present invention can significantly extend the effective duration of sunscreen and reduce the need for re-application.

[0132] The foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A sunscreen product with tripeptide-1 and hydrolyzed wheat protein as active ingredients, characterized in that: The sunscreen product comprises phases A and D that synergistically produce a sunscreen effect, phase B for after-sun repair, and phase C as a light base liquid; Among them, the phase A includes gum arabic, hydrolyzed wheat protein, squalane, tremella polysaccharide, L-arginine hydrochloride, tromethamine, a preservative and deionized water; the phase B includes gellan gum; the phase C includes glycerin, polyglycerol-3 methylglucose distearate, small molecule hyaluronic acid and niacinamide; the phase D includes Centella asiatica extract, tripeptide-1 and Rhodiola rosea extract.

2. The sunscreen product with tripeptide-1 and hydrolyzed wheat protein as active ingredients as claimed in claim 1, characterized in that: The mass ratio of phase A, phase B, phase C and phase D is 100:(0.53-0.65):(10.53-12.87):(5.32-6.50).

3. The sunscreen product with tripeptide-1 and hydrolyzed wheat protein as active ingredients as claimed in claim 1, characterized in that: In terms of weight, every 100 parts of phase A include the following components: 82.42-91.10 parts of deionized water, 0.34-0.37 parts of gum arabic, 2.25-2.48 parts of hydrolyzed wheat protein, 3.93-4.34 parts of squalane, 5.61-6.21 parts of tremella polysaccharide, 0.05-0.07 parts of L-arginine hydrochloride, 0.05-0.07 parts of tromethamine and 0.34-0.37 parts of preservatives.

4. The sunscreen product with tripeptide-1 and hydrolyzed wheat protein as active ingredients as claimed in claim 1, characterized in that: By weight, every 100 parts of the phase D comprises the following components: 15-22 parts of Centella asiatica extract, 18-40 parts of tripeptide-1 and 50-60 parts of Rhodiola rosea extract.

5. The sunscreen product with tripeptide-1 and hydrolyzed wheat protein as active ingredients as claimed in claim 1, characterized in that: In the phase C, the mass ratio of glycerol, polyglyceryl-3 methylglucose distearate, small molecule hyaluronic acid and niacinamide is 3.5:(0.2-0.6):(0.5-1.5):(2-8).

6. The method for preparing a sunscreen product with tripeptide-1 and hydrolyzed wheat protein as active ingredients according to any one of claims 1 to 5, characterized in that: The following steps are involved: The components of phase A and phase C are mixed according to a mass ratio to prepare phase A and phase C; Dissolve phase B with deionized water to form a uniform gellan gum solution; Slowly add the obtained gellan gum solution into phase A and stir to obtain a uniform mixture of phase A and phase B; Slowly add phase C to the obtained mixture of phases A and B, stirring until the phases are evenly mixed; Add the Centella asiatica extract, tripeptide-1 and rhodiola rosea extract in the phase D components into the system in sequence, stir evenly, and obtain the product.

7. The method for preparing a sunscreen product with tripeptide-1 and hydrolyzed wheat protein as active ingredients as claimed in claim 6, characterized in that: The preparation of phase A comprises the following steps: Slowly add gum arabic into the stirred deionized water and stir until it is evenly dispersed; Slowly add hydrolyzed wheat protein, stir until evenly mixed, then add squalane dropwise, stir until completely emulsified to form a uniform emulsion; Add Tremella polysaccharide to the emulsion, stir until the Tremella polysaccharide is fully dissolved, then add L-arginine hydrochloride, tromethamine and a preservative in sequence, stir evenly, and the product is obtained.

8. The method for preparing a sunscreen product with tripeptide-1 and hydrolyzed wheat protein as active ingredients as claimed in claim 6, characterized in that: In the gellan gum solution, the mass ratio of phase B to deionized water is 1:(100-200).

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