Fluorescence microscope with multiple imaging modes

Abstract

The invention provides a fluorescence microscope with multiple imaging modes, which comprises a conversion assembly, the conversion assembly comprises an adjusting mechanism and a translation mechanism coupled to the adjusting mechanism, and the translation mechanism is configured to be driven by the adjusting mechanism to translate upwards at a position perpendicular to a second optical axis of the fluorescence microscope; and the at least one fluorescent assembly is arranged on the translation mechanism in parallel, so that the translation mechanism drives the optical axis of the fluorescent assembly to coincide with the second optical axis of the fluorescence microscope when the translation mechanism translates upwards at a position perpendicular to the second optical axis. The fluorescence microscope is compact in structure and has an adjustable imaging mode.

Description

Technical Field

[0001] The invention relates to the technical field of fluorescence microscopes, in particular to a fluorescence microscope with multiple imaging modes. Background Art

[0002] Currently, microscopes are divided into two categories, namely fluorescence microscopes and ordinary optical microscopes. Ordinary optical microscopes observe samples with ordinary light sources, and can image samples in color and black and white. Unlike ordinary optical microscopes, fluorescence microscopes use light of a certain wavelength (ultraviolet light, blue-violet light) to excite the fluorescent substances in the specimens under the microscope to emit fluorescence. The role of the light source of a fluorescence microscope is not direct illumination, but as an energy source to excite the fluorescent substances in the specimens. The specimens are observed through the fluorescence phenomenon presented by the fluorescent substances in the specimens after absorbing the excitation light energy. The light source of a fluorescence microscope can supply a large amount of excitation light in a specific wavelength range, so that the fluorescent substances in the specimens under examination can obtain the necessary intensity of excitation light.

[0003] After observing and measuring the fluorescence mode of the sample through a fluorescence microscope, the user often needs to observe the black and white or color image of the sample, which requires switching to an ordinary optical microscope for operation. Replacing the sample will cause the position and angle of the sample to change, resulting in the image position observed under the ordinary optical microscope and the image position under the fluorescence microscope not overlapping, seriously affecting the observation effect; and switching between different microscopes is cumbersome and reduces work efficiency. Therefore, it is very necessary to invent a fluorescence microscope with multiple imaging modes. Summary of the invention

[0004] One of the purposes of the present invention is to solve the above-mentioned deficiencies in the prior art and provide a fluorescence microscope with multiple imaging modes.

[0005] To achieve the above-mentioned objectives, some embodiments of the present invention provide a fluorescence microscope with multiple imaging modes, which includes a base plate, a light source support frame arranged on the base plate, a first camera assembly, a conversion assembly, at least one fluorescent assembly, a stage, an objective lens assembly, and an illumination assembly is provided on the light source support frame; wherein the stage, the objective lens assembly, and the illumination assembly are arranged along a first optical axis; the objective lens assembly, the conversion assembly, and the first camera assembly are arranged along a second optical axis that is perpendicular to the first optical axis; wherein the conversion assembly includes an adjustment mechanism and a translation mechanism coupled to the adjustment mechanism, and the translation mechanism is configured to be driven by the adjustment mechanism to translate in a direction perpendicular to the second optical axis; the at least one fluorescent assembly is arranged in parallel on the translation mechanism, so that when the translation mechanism translates in a direction perpendicular to the second optical axis, the optical axis of the one fluorescent assembly is driven to coincide with the second optical axis.

[0006] In some embodiments, a vacant position is provided on the conversion mechanism, so that light of the second optical axis passes through the conversion mechanism and enters the first camera assembly.

[0007] In some embodiments, a second camera assembly is further included which is arranged perpendicular to the first camera assembly. A reflection module is also provided on the translation mechanism of the conversion assembly. The optical axis of the reflection module is parallel to the optical axis of the fluorescent assembly. The reflection module is configured so that when the optical axis of the reflection module driven by the translation mechanism coincides with the second optical axis, the reflection module reflects the light of the second optical axis to the second camera assembly.

[0008] In some embodiments, the empty space is provided between the fluorescent component and the reflective module, or the empty space is provided between two fluorescent components.

[0009] In some embodiments, the conversion assembly further includes a positioning mechanism, and the positioning mechanism is coupled to the translation mechanism to provide positioning for the translation mechanism.

[0010] In some embodiments, the empty space is provided between the reflection module and the positioning mechanism.

[0011] In some embodiments, a focusing component is also included, and the focusing component is coupled to the objective lens component to focus the objective lens component.

[0012] In some embodiments, a display screen is also provided on the light source support frame, and the display screen is communicatively connected with the first camera assembly and / or the second camera assembly.

[0013] In some embodiments, the first camera assembly and the second camera assembly are arranged on the bottom plate at an angle. Preferably, the first camera assembly and the second camera assembly are arranged on the bottom plate perpendicular to each other.

[0014] In some embodiments, the first camera assembly includes a black and white camera assembly and the second camera assembly includes a color camera assembly; or the first camera assembly includes a color camera assembly and the second camera assembly includes a black and white camera assembly;.

[0015] In some embodiments, the translation mechanism includes a first bearing seat and a second bearing seat fixed to the base plate and arranged at intervals, a guide rail and a fluorescent mounting plate arranged in parallel between the first bearing seat and the second bearing seat, and the fluorescent component and the reflection module are fixed to the fluorescent mounting plate.

[0016] In some embodiments, the fluorescent mounting plate includes a side plate portion disposed upright, and the side plate portion is provided with transmission holes for light to pass through. Preferably, the transmission holes are arranged at equal intervals at the same height.

[0017] In some embodiments, the planar portion of the fluorescent mounting plate may further include a positioning post, the position of the positioning post being offset from the position of the light-transmitting hole and corresponding to the non-light-transmitting portion of the side plate portion.

[0018] In some embodiments, the positioning posts are arranged at equal intervals.

[0019] In some embodiments, the adjustment mechanism includes a knob, a gear transmission mechanism fixed to the knob, a lead screw engaged with the gear transmission mechanism, and a nut arranged on the fluorescent mounting plate to cooperate with the lead screw, and the lead screw is arranged between the first bearing seat and the second bearing seat.

[0020] In some embodiments, the positioning mechanism includes a pressure wheel plate arranged between the first bearing seat and the second bearing seat, a pressure wheel seat arranged at the front end of the fluorescent mounting plate, a pressure wheel shaft engaged at the bottom of the pressure wheel seat, and a spring arranged in the pressure wheel seat to bias the pressure wheel shaft; the pressure wheel plate surface has positioning grooves arranged at intervals, and a pressure wheel is arranged on the pressure wheel shaft, and the pressure wheel can be matched and entered into the positioning groove of the pressure wheel plate to limit the position of the fluorescent component and the reflection module.

[0021] In some embodiments, the spacing of the positioning grooves is consistent with the spacing between the optical axes of the fluorescent components, or the spacing between the optical axis of the fluorescent component and the optical axis of the reflective module.

[0022] In some embodiments, the positioning groove is a U-shaped groove or a hemispherical groove.

[0023] The fluorescence microscope in some of the above-mentioned embodiments of the present application has adjustable imaging modes: black and white mode, color mode and fluorescence mode. By turning the knob of the conversion component, the fluorescence component and the reflection module fixed to the fluorescence mounting plate are moved left and right in the horizontal direction, thereby switching the working mode of the fluorescence microscope. The sensitivity is high, and the minimum displacement of the conversion component can meet the mode switching requirements. When working, only the sample needs to be placed on the stage, the knob of the conversion component is turned, and then the imaging mode can be switched for display. The reliability is high and the operation is simple.

[0024] In the fluorescence microscope in some of the above embodiments of the present application, the conversion component has flexible and smooth transmission without backlash, and the adjusted position can be kept stable with high reliability.

[0025] The fluorescence microscope in some of the above embodiments of the present application can accurately switch between multiple modes, namely different fluorescence modes and bright field illumination modes, by providing a positioning mechanism.

[0026] In some embodiments, the camera assembly includes a first imaging lens barrel, a second imaging lens barrel, a camera, a lens barrel mounting seat, a window mirror, a first imaging lens, a second imaging lens, a third imaging lens, a first pressure ring, a first spacer ring, and a focusing knob; wherein the first imaging lens barrel and the second imaging lens barrel are connected by threads, and the second imaging lens barrel is fixed to the bottom plate; the window mirror and the first imaging lens are fixed inside the first imaging lens barrel by the first spacer ring and the first pressure ring; the second imaging lens and the third imaging lens are fixed inside the focusing knob by the first pressure ring, the focusing knob is threadedly connected to the second imaging lens barrel, and the camera is connected and fixed to the second imaging lens barrel. Wherein, the camera is a CMOS camera with high sensitivity and good resolution. Turning the focusing knob can adjust the position of the second imaging lens and the third imaging lens in the lens barrel so that the imaging focal plane is focused at the camera position, so that the observed image is clear and the best observation effect is achieved.

[0027] In some embodiments, the fluorescent assembly includes a fluorescent lens barrel, a heat sink, a light board, a light board seat, a focusing lens barrel, a first imaging lens, a second imaging lens, a third imaging lens, a fourth imaging lens, a second pressure ring, a second spacer, a fluorescent reflector and a fluorescent lens seat; the light board is fixed in the fluorescent lens barrel through the light board seat, the heat sink is connected to the light board, and thermal grease is placed at the connection to dissipate heat from the light board; the first imaging lens to the fourth imaging lens are fixed to the focusing lens barrel through the second pressure ring and the second spacer, and the focusing lens barrel is threadedly fixed to the fluorescent lens barrel; the fluorescent reflector is fixed to the lower side of the fluorescent lens barrel through UV glue.

[0028] The light board as a fluorescent light source has a wide spectrum and can cover a sufficient excitation spectrum; the fluorescent component can reflect light in the excitation band, the excitation light wavelength is 300nm-700nm, and transmit light in the emission band to meet the use requirements.

[0029] In some embodiments, the objective lens assembly includes an objective lens guide seat, a motor, an objective lens guide, an objective lens support frame, a driving gear, a driven gear, a thumbwheel, a gear fixing shaft, an objective lens disk and a plurality of objective lenses threadedly connected to the objective lens disk; the objective lens guide seat is fixed to a base plate, and the motor and the objective lens guide are fixedly connected to the objective lens guide seat; the objective lens disk is fixedly connected to the objective lens support frame and a driven wheel gear at the same time; the driven gear is a helical gear, and the driving gear is a spur gear, and an angle is formed between the driven gear and the driving gear; the driving gear is placed horizontally, and the objective lens disk and the objective lens support frame are fixed to the driven gear and placed obliquely, at this time, the objective lens is located directly below the stage, and the driving gear and thumbwheel are fixed to the base plate through the gear fixing shaft; the rotating thumbwheel is fixedly connected to the driving gear, driving the driven gear to rotate, thereby rotating the objective lens disk to switch objective lenses of different magnifications; the control motor drives the objective lens guide to move up and down, thereby moving the objective lens disk and the objective lens up and down to adjust the focal length of the objective lens to ensure the clarity of the imaging picture.

[0030] In some embodiments, the multiple objective lenses have five specifications according to magnification, namely 2X, 4X, 10X, 20X, and 40X, and are all fixed to the objective lens plate by threaded connection.

[0031] In some embodiments, the angle formed between the driven gear and the driving gear is 150° to 160°, especially 157.5°. Since the heights of objective lenses with different magnifications are different, the above angle design can avoid interference between the objective lens and the stage, while minimizing the height of the objective lens. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1A is a schematic structural diagram of a fluorescence microscope with multiple imaging modes according to an embodiment of the present application;

[0033] Figure 1B is a schematic structural diagram of a fluorescence microscope with multiple imaging modes after removing the stage assembly according to an embodiment of the present application;

[0034] Figure 2A is a schematic structural diagram of a conversion component according to an embodiment of the present application;

[0035] Figure 2B is a schematic structural diagram of a conversion assembly according to an embodiment of the present application after removing a fluorescent assembly, a reflection module and a pressure wheel seat;

[0036] Figure 2C is a schematic structural diagram of a pressure wheel seat in a conversion assembly according to an embodiment of the present application;

[0037] Figure 3 is a schematic structural diagram of a camera assembly according to an embodiment of the present application;

[0038] Figure 4 is a schematic structural diagram of a fluorescent component according to an embodiment of the present application;

[0039] Figure 5 is a schematic structural diagram of an objective lens assembly according to an embodiment of the present application;

[0040] Figure 6 is a schematic structural diagram of a lighting assembly according to an embodiment of the present application;

[0041] Figure 7 is a schematic structural diagram of a stage according to an embodiment of the present application;

[0042] Figure 8 is a structural schematic diagram of a focusing assembly according to an embodiment of the present application;

[0043] Fig. 9is a structural principle diagram of a fluorescence microscope with multiple imaging modes according to an embodiment of the present application, and the figure shows a principle diagram of fluorescence component imaging;

[0044] Fig.10 It is a structural principle diagram of a fluorescence microscope with multiple imaging modes according to an embodiment of the present application, and the figure shows a principle diagram of imaging of a color camera component under bright field illumination.

[0045] Fig.11 It is a structural principle diagram of a fluorescence microscope with multiple imaging modes according to an embodiment of the present application, and the figure shows a principle diagram of imaging of a black and white camera component under bright field illumination. DETAILED DESCRIPTION

[0046] The present invention will be further described below in conjunction with the accompanying drawings and embodiments, but the protection scope of the present invention shall not be limited thereto.

[0047] like Fig. 9 , Fig.10 The specific structural and functional details disclosed herein are merely representative and are for the purpose of describing exemplary embodiments of the present application. However, the present application may be implemented in many alternative forms and should not be construed as being limited to the embodiments described herein.

[0048] It should be understood that, although the terms "first", "second", etc. may be used herein to describe various units, these units should not be limited by these terms. These terms are used only to distinguish one unit from another unit. For example, without departing from the scope of the exemplary embodiments, the first unit may be referred to as the second unit, and similarly the second unit may be referred to as the first unit. The term "and / or" used herein includes any and all combinations of one or more of the listed associated items.

[0049] The terms used herein are only for describing specific embodiments and are not intended to limit exemplary embodiments. Unless the context clearly indicates otherwise, the singular forms "one", "one" and "item" used herein are also intended to include plural numbers. It should also be understood that the terms "include" and / or "comprise" used herein specify the existence of stated features, integers, steps, operations, units and / or components, without excluding the existence or addition of one or more other features, integers, steps, operations, units, components and / or combinations thereof.

[0050] It should also be noted that, in some alternative implementations, the functions / actions mentioned may occur in a different order than that indicated in the accompanying drawings. For example, depending on the functions / actions involved, two figures shown in succession may actually be performed substantially simultaneously or may sometimes be performed in the reverse order.

[0051] Fig.11 As shown, some embodiments of the present invention provide a fluorescence microscope with multiple imaging modes, which includes a base plate 1, a light source support frame 2 arranged on the base plate 1, a black and white camera assembly 3, a color camera assembly 4, a conversion assembly 5, a fluorescent assembly 6, a stage 7, an objective lens assembly 8, and an illumination assembly 10 is provided on the light source support frame; wherein the stage 7, the objective lens assembly 8, and the illumination assembly 10 are arranged along a first optical axis A1; the objective lens assembly 8, the conversion assembly 5, and the color camera assembly 4 are arranged along a second optical axis A2 that is perpendicular to the first optical axis. Among them, the conversion component 5 includes an adjustment mechanism 51 and a translation mechanism 52 coupled to the adjustment mechanism 51, and the translation mechanism 52 is configured to be driven by the adjustment mechanism 51 to translate in a direction A2 that is perpendicular to the second optical axis A2; the fluorescent component 6 and the reflection module 19 are arranged in parallel on the translation mechanism 52, and an empty space is provided in the middle, so that when the translation mechanism 52 translates in a direction A3 that is orthogonal to the second optical axis A2, the optical axis B1 of the fluorescent component 6, the optical axis B2 of the empty space, and the optical axis B3 of the reflection module 19 are driven to coincide with the second optical axis A2 respectively, thereby forming an image on the black and white camera component 3 and the color camera component 4.

[0052] The specific structure of the microscope in one embodiment of the present application can be shown in FIG1, as shown in the figure, it includes a bottom plate 1, a light source support frame 2, a black and white camera assembly 3, a color camera assembly 4, a conversion assembly 5, a fluorescent assembly 6, a stage 7, an objective lens assembly 8, a focusing assembly 9, an illumination assembly 10 and a display screen 11. The light source support frame 2, the black and white camera assembly 3, the color camera assembly 4, the conversion assembly 5, the stage 7, the objective lens assembly 8, and the focusing assembly 9 are fixedly connected to the bottom plate 1; the illumination assembly 10 and the display screen 11 are connected and fixed to the light source support frame 2.

[0053] like Figure 2A , Figure 2B , Figure 2C The structure diagram of the conversion assembly 5 is shown, the translation mechanism 501 includes a first bearing seat 12 and a second bearing seat 22 fixed to the bottom plate 1 and arranged at intervals, a guide rail 15 and a fluorescent mounting plate 21 arranged in parallel between the first bearing seat 12 and the second bearing seat 22, and a fluorescent assembly 6 and a reflection module 19 fixed to the fluorescent mounting plate 21. The adjustment mechanism 502 includes a knob 24, a gear transmission mechanism 23 fixed to the knob 24, a lead screw 13 engaged with the gear transmission mechanism 23, and a nut arranged on the fluorescent mounting plate to cooperate with the lead screw 13, and the lead screw 13 is arranged between the first bearing seat 12 and the second bearing seat 22.

[0054] The first bearing seat 12 and the second bearing seat 22 of the bearing bearing are arranged at intervals and are both fixed to the bottom plate 1. The lead screw 12, the pressure wheel plate 14 and the guide rail 15 are arranged between the first bearing seat 12 and the second bearing seat 22, especially in parallel. Figure 2A , Figure 2B , Figure 2C In the illustrated embodiment, the lead screw 12 and the guide rail 15 are disposed on both sides of the pressure roller plate 14, wherein the two ends of the lead screw 12 are connected to the first bearing seat and the second bearing seat respectively through bearings. The fluorescent mounting plate 21 is matched with the guide rail 15 and the lead screw 12, for example, the first side of the fluorescent mounting plate 21 has a nut, which is sleeved on the lead screw 12 and meshed with the lead screw 12, and the second side of the fluorescent mounting plate 21 is matched with the guide rail 15 so that the fluorescent mounting plate 21 can move along the lead screw and the guide rail 15 along the upper surface of the pressure roller plate 14 between the first bearing seat 12 and the second bearing seat 22. The fluorescent mounting plate 21 includes a rear section that matches the guide rail 15 and the lead screw 12, and a front section for mounting the pressure roller seat 18, wherein the front section is arranged at a distance from the pressure roller plate 14, for example, arranged in parallel at a first distance.

[0055] like Figure 2A , 2B As shown, the fluorescent mounting plate 21 includes a side plate portion arranged upright, and a transmission hole 211 for light to pass through is arranged on the side plate portion. The transmission hole 211 can block the entry of stray light, and the transmission holes 211 can be arranged at equal intervals at the same height. The flat portion of the fluorescent mounting plate 21 can also include a positioning column 212, and the position of the positioning column deviates from the position of the light-transmitting hole 211, corresponding to the non-light-transmitting portion of the side plate portion. The positioning column 212 can be arranged at equal intervals on the same straight line. The positioning column can be used to position the fluorescent component 6 and / or the reflection module 19. The positioning column and the transmission hole 211 are arranged in pairs.

[0056] A plurality of fluorescent components 6 and a reflective module 19 are fixed to the fluorescent mounting plate 21 and move together with the fluorescent mounting plate 21, wherein the reflective module 19 is fixed to a side of the fluorescent mounting plate 21 adjacent to the first bearing seat 12, and a plurality of fluorescent components 6 are fixed side by side to a side of the fluorescent mounting plate 21 adjacent to the second bearing seat 22. An empty space is left between the fluorescent components 6 and the reflective module 19 on the fluorescent mounting plate 21. It should be understood that the empty space can be set at any position, for example, between the fluorescent component and the reflective module, between the reflective module and the positioning mechanism, or between the fluorescent components.

[0057] A pressure wheel seat 18 is arranged on the front side of the reflection module 19 on the fluorescent mounting plate 21, i.e., closer to the first bearing seat 12. The lower surface of the pressure wheel seat 18 extends to a certain distance above the surface of the pressure wheel plate 14, for example, 1 to 5 mm. A longitudinal shaft hole is arranged inside the pressure wheel seat 18, and a spring 16 is arranged inside the longitudinal shaft hole. The spring 16 is a stainless steel compression spring with a wire diameter of 3 mm. A transverse shaft groove 181 connected to the longitudinal shaft hole is arranged near the lower surface of the pressure wheel seat 18. A pressure wheel shaft 17 is arranged in the transverse shaft groove. A pressure wheel 171 is arranged on the pressure wheel shaft. The radial dimension of the middle part of the transverse shaft groove is expanded to form a wheel groove 182. The pressure wheel shaft 17 is biased in the transverse shaft groove by the spring 16, so that the pressure wheel extends out of the lower surface of the pressure wheel seat 18 and is biased on the pressure wheel plate 14.

[0058] One end of the main shaft of the gear transmission mechanism 23 is fixed to the knob 24, and the other end of the main shaft is fitted in the bearing of the second bearing seat 22. The gear transmission mechanism 23 is meshed with the lead screw 12, so that by turning the knob 24, the gear rotation mechanism 23 can be driven to drive the fluorescent mounting plate 21 to move horizontally along the guide rail 15, so that the pressure wheel seat 18 moves along the upper surface of the pressure wheel plate 14.

[0059] In the above structure, the pressure wheel plate 14, the pressure wheel seat 18, and the spring 16 and the pressure wheel shaft 17 in the pressure wheel seat constitute an optional positioning mechanism 503 for precise positioning. Positioning can also be achieved by other positioning means, such as by arranging a position sensor at a predetermined position to provide a positioning signal to achieve positioning.

[0060] In the above structure, the movement of the fluorescent mounting plate 21 can be realized by replacing the lead screw 12 and the gear transmission structure with a transmission device such as a transmission belt and a pulley, so as to switch the imaging mode. The knob 24 can be coupled to the pulley to realize the switching of the imaging mode.

[0061] There are positioning grooves in the form of U-shaped grooves 141 with a predetermined spacing on the upper surface of the pressure wheel plate 14. The predetermined spacing can be 20 to 40 mm, especially 30 mm, and the pressure wheel plate 14 is fixed to the bottom plate 1 through the mounting hole 142 on the pressure wheel plate. Every time the pressure wheel seat 18 moves 30 mm, the pressure wheel 171 of the pressure wheel shaft 17 of the pressure wheel seat 18 will be stuck in the U-shaped groove 141 of the pressure wheel plate 14 due to the rebound pressure of the spring 16, thereby limiting the movement position of the fluorescent component 6, the empty space and the reflection module 19, thereby playing the role of switching the working mode of the microscope.

[0062] It should be understood that the above spacing is only to make the mode switching of the microscope more accurate, and the spacing can be adjusted according to actual conditions. The spacing of the positioning grooves can be set according to the adjustment mechanism. For ordinary knob adjustment mechanisms, equal spacing is ideal. In addition to the U-shaped groove, the positioning groove can also be a hemispherical groove.

[0063] Each fluorescent component 6 can reflect light in the excitation band, the excitation light wavelength is 300nm to 700nm, and transmit light in the emission band to meet the use requirements. The fluorescent component 6 is designed to be more compact to adapt to the conversion component 5. The structure of the fluorescent component in this embodiment is as follows Figure 4 As shown, it includes a heat sink 36, a fluorescent lamp board 37, a lamp board seat 38, a fluorescent lens barrel 39, a focusing lens barrel 40, a fourth imaging lens 41, a fifth imaging lens 42, a second spacer 43, a sixth imaging lens 44, a seventh imaging lens 45, a second pressing ring 46, a fluorescent reflector 47, and a fluorescent lens seat 48. The lamp board 37 is fixed in the fluorescent lens barrel 39 through the lamp board seat 38. The heat sink 36 is connected to the lamp board 37, and thermal conductive silicone grease is placed at the connection to dissipate heat for the lamp board 37; the fourth imaging lens 41 to the seventh imaging lens 45 are fixed to the focusing lens barrel 40 through the second pressing ring 46 and the second spacer 43, and the focusing lens barrel 40 and the fluorescent lens barrel 39 are fixed by threaded connection; the fluorescent reflector 47 is fixed to the lower side of the fluorescent lens barrel 39 by UV glue. As a fluorescent light source, the lamp board 37 has a wide spectrum and can cover a sufficient excitation spectrum; the fluorescent lens seat 48 is connected to the fluorescent lens barrel 39 as a whole. The fluorescent component is mounted on the fluorescent mounting plate 21 through the fluorescent lens holder 48. The fluorescent component 6 can be a conventional fluorescent component, and the lamp beads on the fluorescent lamp board 37 thereof emit excitation light with a wavelength of A, which is collimated by a collimating lens group (composed of a fourth imaging lens 41, a fifth imaging lens 42 and a sixth imaging lens 44), and then reflected by a dichroic mirror (i.e., a fluorescent reflector 47) to hit the object to be measured. The atoms inside the object to be measured absorb the excitation light and enter an excited state, and then release fluorescence with a wavelength of B (B>A). After passing through the dichroic mirror and the filter, the fluorescence enters the imaging tube lens, and then forms a fluorescence image on the camera CMOS.

[0064] like Figure 7 The structure diagram of the stage 7 is shown, including an X-axis moving knob 70, a Y-axis moving knob 71, an upper plate 72, a middle plate 73, a lower plate 74, a loading plate 75, and a loading stage bracket 76. The X-axis moving knob 70 is fixedly connected to the upper plate 72, and the Y-axis moving knob 71 is fixedly connected to the middle plate 73. The X-axis moving knob 70 is rotated to drive the upper plate 72 to move left and right in the horizontal direction, and the Y-axis moving knob 71 is rotated to drive the middle plate 73 to move forward and backward in the horizontal direction. The loading plate 75 is fixed to the upper plate 72 and is used to place the sample to be observed. The lower plate 74 is directly fixed to the bottom plate 1 through the loading stage bracket 76. The loading plate 75 is made of flexible material as a whole, is light in weight, has good vibration isolation effect, can effectively isolate vibration, prevent the fluorescence microscope from shaking due to external vibration, and ensure the stability of the imaging picture.

[0065] like Figure 5FIG. 8 is a schematic diagram of the structure of the objective lens assembly 8, which is an objective lens assembly with a compact structure design with a small height. Figure 5 As shown, the objective lens assembly 8 includes an objective lens guide seat 49, an objective lens group motor 50, an objective lens guide 51, an objective lens support frame 52, an objective lens 53, an objective lens disk 54, a driven gear 55, a driving gear 56, a thumbwheel 57, and a gear fixing 58, wherein the objective lens guide seat is fixed to the bottom plate 1, and the motor 50 is fixedly connected to the objective lens guide 51 and the objective lens guide seat 49. The objective lens has five specifications according to the magnification, namely 2X, 4X, 10X, 20X, and 40X, and is fixed to the objective lens disk 54 by threaded connection. The objective lens disk 54 is also fixedly connected to the objective lens support frame 52 and the driven gear 55; the driven gear 55 is a helical gear, and the driving gear 56 is a spur gear. The angle between the driven gear 55 and the driving gear 56 is 150° to 160°, especially 157.5°. The driving gear 56 is placed horizontally, and the objective lens disk 54 and the objective lens support frame 52 are fixed to the driven gear 55 and placed obliquely. At this time, the objective lens is located directly below the stage. Since the heights of the objective lenses with different magnifications are different, a 157.5° angle design is adopted to avoid interference between the objective lens and the stage, and at the same time, the height of the objective lens is minimized. The driving gear 56 and the dial wheel 57 are fixed to the bottom plate 1 through the gear fixing shaft 58. Rotating the dial wheel 57 can rotate the driving gear 56, drive the driven gear 55 to rotate, and thus rotate the objective lens disk 54 to switch the objective lens 53 with different magnifications to the working position, that is, the position aligned with the optical axis of the lighting assembly 10. The motor 50 receives the feedback signal from the focusing assembly 9, drives the objective lens guide 51 to move up and down relative to the objective lens guide seat 49, so that the objective lens disk 54 and the objective lens 53 move up and down as a whole, thereby adjusting the focal length of the objective lens to ensure the clarity of the imaging picture.

[0066] A reflector 81 is arranged below the objective lens assembly 8, and the reflector 81 is arranged on the optical axis of the lighting assembly 10 and the objective lens 53 at the working position. The reflector is configured to reflect light from the fluorescent assembly 6 to the objective lens 53 at the working position, and to reflect light from the objective lens at the working position (i.e., fluorescence reflected by the sample or bright field light) to the fluorescent assembly.

[0067] like Figure 8 As shown, the focusing assembly 9 includes a focusing knob 77, an objective lens long axis bracket 78, an objective lens long axis 79, an objective lens encoder 80 and an encoder bracket 81. The focusing assembly 9 is fixed to the bottom plate 1 as a whole, and is located on the side of the objective lens assembly 8. The focusing knob and the objective lens long axis 79 are fixedly connected, and the turning of the knob can drive the objective lens long axis 79 to rotate. The objective lens encoder 80 fixed on the encoder bracket 81 will read the angle of rotation of the focusing knob to provide a feedback signal to the objective lens group motor 50, and drive the objective lens group to move up and down to achieve the effect of adjusting the focal length.

[0068] The present application can use any illumination assembly to provide bright field light for the microscope, however, Figure 6 The lighting assembly 10 has a better effect. Figure 6 As shown, the lighting assembly 10 includes a lighting lampshade 59, a lighting lamp board 60, a first lighting lens barrel outer cover 61, a lighting mounting seat 62, a first lighting lens barrel 63, a lighting lens group 64, a second lighting lens barrel 65, a phase difference ring fixing plate 66, a third lighting lens barrel 67, a fourth lighting lens barrel 68, and a window lens group 69. Among them, the lighting lamp board 60 is fixed to the inside of the lighting lampshade 59 by UV glue, the first lighting lens barrel outer cover 61 is fixedly connected to the lighting lampshade 59, the first lighting lens barrel 63, the second lighting lens barrel 65, the third lighting lens barrel 67, the fourth lighting lens barrel 68 and the phase difference ring fixing plate 66 are all fixedly connected to the first lighting lens barrel outer cover 61 by threads, the lighting lens group 64 is placed inside the first lighting lens barrel 63, and the window lens group 69 is placed inside the third lighting lens barrel 67. The light source provided by the lighting assembly in this embodiment has high brightness and good uniformity, and has sufficient illumination.

[0069] The black and white camera component 3 and the color camera component 4 may have the same structure, but use different CMOS sensors. Figure 3As shown, the structure of the black and white camera assembly 3 includes a first pressing ring 25, a first imaging lens 26, a first spacer ring 27, a first imaging lens barrel 28, a second imaging lens 29, a second imaging lens barrel 30, a focusing knob 31, a lens barrel mounting seat 32, a third imaging lens 33, a window mirror 34, and a camera 35. The first imaging lens barrel 28 and the second imaging lens barrel 30 are connected by threads, the second imaging lens barrel 30 is sleeved in the lens barrel mounting seat 32, and the lens barrel mounting seat 32 is fixed on the bottom plate 1; the window mirror 34 and the first imaging lens 26 are fixed inside the first imaging lens barrel 28 by the first spacer ring 27 and the first pressing ring 25; the second imaging lens 29 and the third imaging lens 33 are fixed inside the focusing knob 31 by the pressing ring, the focusing knob 31 is threadedly connected to the second imaging lens barrel 30, and the camera part 35 is connected and fixed to the second imaging lens barrel 30. The camera is a CMOS camera, the pixel size is, for example, 3.45um×3.45um, with high sensitivity and good resolution. The positions of the second imaging lens 29 and the third imaging lens 33 in the second imaging lens barrel 30 can be adjusted by rotating the focus knob 31, so that the imaging focal plane is focused at the position of the camera 35 to achieve the best observation effect. The black and white camera assembly 3 and the color camera assembly 4 can be arranged perpendicular to each other, and the reflector in the reflection module 19 can be arranged at the mutually perpendicular optical axis focal points of the black and white camera assembly 3 and the color camera assembly 4, so that the reflection module can reflect the light of the second optical axis to the black and white camera assembly 3 for imaging. The black and white camera assembly 3 and the color camera assembly 4 can also be separately arranged on the optical axis of the fluorescent assembly 6 or the reflective module 19 on the exit side, thereby avoiding the use of the reflection module.

[0070] In addition to being arranged perpendicularly to each other, the black and white camera assembly 3 and the color camera assembly 4 can also be arranged at any angle to adapt to the layout of the components on the base plate of the microscope. The light from the second optical axis can be reflected into the black and white camera assembly 3 by simply adjusting the angle of the reflector in the reflection module.

[0071] In addition, it should be understood that the black and white camera component 3 and the color camera component 4 can also be swapped, and the black and white camera component 3 defines a second optical axis, while the color camera component 4 is set at an angle to the black and white camera component 3 and receives reflected light from the reflection module 19.

[0072] The display screen 11 in the present application can be a display screen of any resolution / format, such as standard definition resolution, high definition resolution, 2K, 4K high definition resolution; in some embodiments, the display screen 11 can be a 2K high definition display screen, which has higher definition than a standard definition display screen. The display screen is connected to the black and white camera assembly / color camera assembly through a signal line, and the camera in the black and white camera assembly / color camera assembly forms an image and transmits the image to the display screen.

[0073] A sample is placed on the stage 7, the light source of the lighting assembly 10, the sample, and the reflector 81 of the objective lens 8 are on the same vertical first optical axis, the reflector 81 of the objective lens 8 and the color camera assembly 4 are on the second optical axis, and the black and white camera assembly 3 and the color camera assembly 4 are arranged perpendicular to each other. In the transmission bright field illumination mode, the conversion assembly 5 is adjusted so that the second optical axis defined by the color camera assembly 4 and the reflector 81 passes through the empty position of the conversion assembly 5, then the light source of the bright field illumination assembly 4 emits light, the light passes through the sample, the objective lens 8, the reflector 81 in sequence, and finally forms an image in the color camera assembly 4. The conversion assembly 5 is adjusted so that the optical axis of the reflection module 19 is at the position where the second optical axis defined by the color camera assembly 4 and the reflector 81 coincides, then the light source of the bright field illumination assembly 4 emits light, the light passes through the sample, the objective lens 8, the reflector 81 in sequence, and finally forms an image in the black and white camera assembly 3. When exciting the fluorescent component, the lighting component 10 is turned off, and the conversion component 5 is adjusted so that the optical axis of the fluorescent component 6 coincides with the second optical axis. At this time, the fluorescent component 6 emits fluorescence, and the light passes through the objective lens 8 to the sample, and then is reflected from the sample and passes through the reflector 81 of the objective lens 8, the fluorescent component 6, and finally forms an image in the color camera component 4.

[0074] The present invention has three imaging modes: black and white mode, color mode and fluorescence mode. There is no need to switch different microscopes. The imaging mode of the microscope can be adjusted by simply turning the knob 24 of the conversion component 5. The operation is simple. Compared with the traditional fluorescence microscope, the present invention has the following advantages:

[0075] First, it has three imaging modes: black and white mode, color mode, and fluorescence mode. There is no need to switch between different microscopes. The imaging mode of the microscope can be adjusted by simply turning the knob, which is easy to operate.

[0076] Second, the imaging mode is controlled by the conversion component 5, the structural transmission of the conversion component 5 is flexible and smooth, no backlash is generated, and the adjusted position can be kept stable with high reliability.

[0077] Third, the stage of the fluorescence microscope of the present invention is made of flexible material, is light in weight, and has good vibration isolation effect, which can effectively isolate vibration and prevent the fluorescence microscope from shaking due to external vibration, and the imaging picture has good stability.

[0078] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the claims and their equivalents.

Claims

1. A fluorescence microscope with multiple imaging modes, characterized in that: It comprises a base plate, a light source support frame arranged on the base plate, a first camera assembly, a conversion assembly, at least one fluorescent assembly, an object stage, an objective lens assembly, and an illumination assembly is arranged on the light source support frame; wherein the object stage, the objective lens assembly, and the illumination assembly are arranged along a first optical axis; the objective lens assembly, the conversion assembly, and the first camera assembly are arranged along a second optical axis perpendicular to the first optical axis; wherein the conversion assembly comprises an adjustment mechanism and a translation mechanism coupled to the adjustment mechanism, and the translation mechanism is configured to be driven by the adjustment mechanism to translate in a direction perpendicular to the second optical axis; the at least one fluorescent assembly is arranged in parallel on the translation mechanism, so that when the translation mechanism translates in a direction perpendicular to the second optical axis, the optical axis of the one fluorescent assembly is driven to coincide with the second optical axis.

2. The fluorescence microscope with multiple imaging modes according to claim 1, characterized in that: The conversion assembly also includes a positioning mechanism, which is coupled to the translation mechanism to provide positioning for the translation mechanism.

3. The fluorescence microscope with multiple imaging modes according to claim 2, characterized in that: A vacant position is provided on the conversion mechanism, so that the light of the second optical axis passes through the conversion mechanism and enters the first camera assembly.

4. The fluorescence microscope with multiple imaging modes according to claim 3, characterized in that: It also includes a second camera assembly that is arranged perpendicular to the first camera assembly. A reflection module is also provided on the translation mechanism of the conversion assembly. The optical axis of the reflection module is parallel to the optical axis of the fluorescent assembly. The reflection module is configured so that when the optical axis of the reflection module driven by the translation mechanism coincides with the second optical axis, the reflection module reflects the light of the second optical axis to the second camera assembly.

5. The fluorescence microscope with multiple imaging modes according to claim 4, characterized in that: The empty space is provided between the fluorescent component and the reflecting module, or the empty space is provided between two fluorescent components, or provided between the reflecting module and a positioning mechanism.

6. The fluorescence microscope with multiple imaging modes according to claim 1, characterized in that: It also includes a focusing component, which is coupled to the objective lens component and is used to focus the objective lens component.

7. The fluorescence microscope with multiple imaging modes according to claim 1, characterized in that: A display screen is also provided on the light source support frame, and the display screen is communicatively connected with the first camera assembly and / or the second camera assembly.

8. The fluorescence microscope with multiple imaging modes according to claim 1, characterized in that: The first camera assembly and the second camera assembly are arranged on the bottom plate at an angle; preferably, the first camera assembly and the second camera assembly are arranged on the bottom plate perpendicular to each other.

9. The fluorescence microscope with multiple imaging modes according to claim 1, characterized in that: The first camera assembly includes a black and white camera assembly, and the second camera assembly includes a color camera assembly; or the first camera assembly includes a color camera assembly, and the second camera assembly includes a black and white camera assembly.

10. The fluorescence microscope with multiple imaging modes according to claim 1, characterized in that: The translation mechanism comprises a first bearing seat and a second bearing seat fixed to a bottom plate and arranged at intervals, a guide rail and a fluorescent mounting plate arranged in parallel between the first bearing seat and the second bearing seat, and the fluorescent component and the reflection module are fixed to the fluorescent mounting plate.

11. The fluorescence microscope with multiple imaging modes according to claim 10, characterized in that: The fluorescent mounting plate comprises a side plate portion arranged upright, and the side plate portion is provided with transmission holes for light to pass through. Preferably, the transmission holes are arranged at equal intervals at the same height.

12. The fluorescence microscope with multiple imaging modes according to claim 11, characterized in that: The flat portion of the fluorescent mounting plate may further include a positioning post, the position of which deviates from the position of the light-transmitting hole and corresponds to a light-impermeable portion of the side plate.

13. The fluorescence microscope with multiple imaging modes according to claim 12, characterized in that: The positioning posts are arranged at equal intervals.

14. The fluorescence microscope with multiple imaging modes according to claim 1, characterized in that: The adjustment mechanism includes a knob, a gear transmission mechanism fixedly connected to the knob, a lead screw engaged with the gear transmission mechanism, and a nut arranged on the fluorescent mounting plate and matching with the lead screw, wherein the lead screw is arranged between the first bearing seat and the second bearing seat.

15. The fluorescence microscope with multiple imaging modes according to claim 2, characterized in that: The positioning mechanism includes a pressure wheel plate arranged between the first bearing seat and the second bearing seat, a pressure wheel seat arranged at the front end of the fluorescent mounting plate, a pressure wheel shaft matched at the bottom of the pressure wheel seat, and a spring arranged in the pressure wheel seat to bias the pressure wheel shaft; the surface of the pressure wheel plate has positioning grooves arranged at intervals, and a pressure wheel is arranged on the pressure wheel shaft, and the pressure wheel can be matched and entered into the positioning groove of the pressure wheel plate to limit the position of the fluorescent component and the reflection module.

16. The fluorescence microscope with multiple imaging modes according to claim 15, characterized in that: The spacing of the positioning grooves is consistent with the spacing between the optical axes of the fluorescent components, or the spacing between the optical axis of the fluorescent component and the optical axis of the reflective module.

17. The fluorescence microscope with multiple imaging modes according to claim 16, characterized in that: The positioning groove is a U-shaped groove or a hemispherical groove.

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