Microparticle, NK cell activator, method for culturing NK cells, method for producing activated NK cells, and method for activating NK cells

By using microparticles from the culture supernatant from the pulp or adipose stem cell, including miRNAs that regulate NK cell activation related genes, the problem of failure to effectively regulate NK cell activation in the prior art is solved, and effective activation of NK cells and enhanced immune responses are achieved.

CN119998443APending Publication Date: 2025-05-13DEXON PHARM INC
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Patent Information

Application Number
CN202380069346.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Priority Date
2022-09-28
Filing Date
2023-08-27
Publication Date
2025-05-13

AI Technical Summary

Technical Problem

The prior art has failed to effectively provide methods for regulating protein and/or gene expression associated with NK cell activation, especially exosomes from mesenchymal stem cells for the regulation of miR-155.

Method used

By using particles containing specific microRNAs, especially those derived from stem cell culture supernatant from pulp or fat, these particles can regulate the expression of proteins and/or genes associated with NK cell activation, specifically miRNAs containing hsa-miR-155-5p as target genes.

Benefits of technology

Effective activation of NK cells is achieved, and the destructive ability of NK cells is promoted by increasing the expression of granzyme B, thereby potentially enhancing the defense of immune responses against cancer and viral infection.

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Abstract

The present invention provides: novel microparticles which contain miRNA related to NK cell activation, and which are derived from a culture supernatant of dental pulp-derived stem cells or a culture supernatant of adipose-derived stem cells, and which are capable of regulating the expression of proteins and / or genes related to NK cell activation; an NK cell activator; a culture method of NK cells; a method for producing activated NK cells; an NK cell; the invention relates to a method for activating NK cells.
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Citation Information

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