Environment-friendly root stock grafted plant cultivation method

Abstract

The invention relates to the technical field of plant grafting, and discloses an environment-friendly root stock grafted plant cultivation method which comprises the following steps: S1, selecting stocks and scions: selecting healthy stocks and scions without plant diseases and insect pests, trimming the scions needing to be grafted, and removing leaves and redundant buds; s2, stock and scion pretreatment; s3, grafting operation; s4, carrying out symbiotic culture on probiotics; s5, carrying out multi-layer binding; s6, healing period management. A plurality of layers of protective films are used for binding the grafting position, the first layer of cellulose film is soaked in a probiotic solution, wound healing is promoted, film tearing damage can be degraded, the environmental protection property of grafting is improved, meanwhile, the second layer of polyurethane film has elasticity, air permeability and protection effects, and the third layer of cotton film increases moisture retention and comfort. By means of multi-layer cooperation, a stable, moist and sterile environment is created, the healing speed is increased, and the growth quality is guaranteed.

Description

Technical Field

[0001] The invention relates to the technical field of plant grafting, in particular to an environmentally friendly rootstock grafted plant cultivation method. Background Art

[0002] In orchards, rot is highly contagious. Once it breaks out, it spreads rapidly, causing the bark and branches of fruit trees to rot, the vascular system to be damaged, the nutrient and water transport to be blocked, and the fruit yield to drop sharply. Many orchards have been severely damaged or even faced destruction, seriously affecting the nutrient transport and water conduction of the trees, which in turn led to a sharp drop in fruit yield. Root grafting technology can use healthy tree roots as rootstocks in orchard reconstruction, and quickly build a new growth system with high-quality scion, which can enhance the absorption capacity and growth vitality of fruit trees, effectively resist rot, and at the same time significantly shorten the seedling cycle to help orchards resume production.

[0003] However, in the current technology, traditional grafting protection measures are single and it is difficult to achieve multiple functions of moisturizing, breathable, antibacterial and damage prevention at the same time. It is impossible to create a stable, moist and sterile ideal environment for the grafting site, thus affecting the healing speed and quality of the interface, resulting in a low grafting survival rate. Summary of the invention

[0004] In view of the shortcomings of the prior art, the present invention provides an environmentally friendly rootstock grafted plant cultivation method to solve the problem that traditional grafting protection measures are single and difficult to achieve multiple protection functions at the same time, resulting in a low grafting survival rate.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: an environmentally friendly root stock grafted plant cultivation method, comprising the following steps:

[0006] S1. Select rootstock and scion: Select healthy rootstock and scion without diseases and insect pests, and then prune the scion to be grafted to remove leaves and excess buds;

[0007] S2. Pretreatment of stock and scion: prepare a biofungicide solution and a plant growth regulator solution, flatten the stock with a knife to form a grafting incision, and soak or rinse the stock incision in a diluted biofungicide solution, then use a sterilized knife to cut the bottom of the scion into a wedge or bevel, the cut surface should be smooth and the length should match the incision of the stock, and soak the incision of the scion in a solution containing a plant growth regulator;

[0008] S3, grafting operation: insert the processed scion into the flattened cut of the stock, so that the cambium of the scion and the stock are aligned;

[0009] S4, probiotic symbiotic culture: prepare probiotic solution and use spray method to evenly distribute the probiotics around the rootstock and scion;

[0010] S5. Multi-layer wrapping: Wrap the grafting site of the rootstock and scion with three layers of protective film, and soak the first layer of protective film in a probiotic solution;

[0011] S6. Management during the healing period: Control the growth environment of the grafted plants, and untie the outer protective film in time according to the growth conditions of the plants to check the healing status of the interfaces.

[0012] Preferably, the rootstock includes roots left after transplanting and tree roots are directly grafted, and the scion in S1 only retains 1-2 buds on the top.

[0013] Preferably, the biofungicide solution in S2 includes carbendazim wettable powder in a ratio of 500-1000 times liquid, and the stock incision is soaked in the carbendazim solution for 10-15 minutes, and then the stock is placed in a well-ventilated place without direct sunlight to dry naturally.

[0014] Preferably, the plant growth regulator solution in S2 includes 100-200 mg / L indolebutyric acid and 50-100 mg / L naphthylacetic acid, and the scion incision is immersed in the plant growth regulator solution for 3-5 minutes.

[0015] Preferably, in S3, if the thickness of the scion and the stock are inconsistent, the cambium on one side of the scion and the stock is aligned.

[0016] Preferably, the multi-layer protective film in S5 comprises a cellulose film, a polyurethane film and a cotton protective film, and the cellulose film is soaked in the probiotic solution for 5 to 10 minutes.

[0017] Preferably, in S6, the temperature of the plant after grafting is controlled at 18-28°C.

[0018] The present invention provides an environmentally friendly rootstock grafted plant cultivation method having the following beneficial effects:

[0019] 1. The present invention uses a multi-layer protective film to wrap the grafting site. The first layer of cellulose film is immersed in a probiotic solution to promote wound healing and prevent damage to the degradable tear film, thereby improving the environmental friendliness of the grafting. At the same time, the second layer of polyurethane film has elasticity, air permeability and protective effects. The third layer of cotton film increases moisture retention and comfort, and the cotton film can be untied to observe the growth of the graft. The multiple layers work together to create a stable, moist and sterile environment, increase the healing speed, and ensure the growth quality.

[0020] 2. The present invention uses Bacillus subtilis, glucose and acetate buffer to first achieve fine dispersion of probiotics by spraying, allowing the probiotics in the solution to fully contact the plant surface in the form of tiny droplets. At the same time, by soaking the cellulose membrane and bundling the grafted part, an effective protective layer can be formed at the grafted part, thereby accelerating wound healing and improving the survival rate and growth quality.

[0021] 3. The present invention prepares carbendazim solution and plant growth regulator solution to soak or rinse the stock incision and the scion incision, which can stimulate cell division and growth and avoid drying of external bacteria, enhance cell vitality, and promote rapid proliferation of cambium cells, thereby improving the survival rate and healing speed, improving the growth quality of the plant, and achieving a good fusion effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 The present invention is a flow chart of the environmentally friendly rootstock grafted plant cultivation method. DETAILED DESCRIPTION

[0023] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] Please see attached Figure 1 The embodiment of the present invention provides an environmentally friendly rootstock grafted plant cultivation method, comprising the following steps:

[0025] S1. Select rootstock and scion: Select healthy rootstock and scion without diseases and insect pests, and then prune the scion to be grafted to remove leaves and excess buds;

[0026] S2. Pretreatment of stock and scion: prepare a biofungicide solution and a plant growth regulator solution, flatten the stock with a knife to form a grafting incision, and soak or rinse the stock incision in a diluted biofungicide solution, then use a sterilized knife to cut the bottom of the scion into a wedge or bevel, the cut surface should be smooth and the length should match the incision of the stock, and soak the incision of the scion in a solution containing a plant growth regulator;

[0027] S3, grafting operation: insert the processed scion into the flattened cut of the stock, so that the cambium of the scion and the stock are aligned;

[0028] S4, probiotic symbiotic culture: prepare probiotic solution and use spray method to evenly distribute the probiotics around the rootstock and scion;

[0029] S5. Multi-layer wrapping: Wrap the grafting site of the rootstock and scion with three layers of protective film, and soak the first layer of protective film in a probiotic solution;

[0030] S6. Management during the healing period: Control the growth environment of the grafted plants, and untie the outer protective film in time according to the growth conditions of the plants to check the healing status of the interfaces.

[0031] Specifically, in S1, ensuring the health of the rootstock and scion and being free of diseases and pests can reduce the risk of disease infection and pests in the grafted plants from the source, improve the grafting success rate and the survival rate of the plants, and remove leaves and excess buds from the scion, which can effectively reduce the evaporation of water and nutrient consumption of the scion, so that the scion can use limited resources for fusion with the rootstock and the generation of new tissues, thereby promoting rapid healing of the interface, improving the quality and efficiency of the graft, and ultimately achieving the goal of cultivating healthy, strong, and high-quality grafted plants;

[0032] In S2, a biofungicide solution is prepared and the stock incision is soaked or rinsed. On the one hand, soaking can effectively kill the pathogens that may exist at the stock incision, reduce the probability of infection after grafting, and create a good sterile environment for interface healing. On the other hand, the biofungicide solution can be used to clean the soil of the stock incision while disinfecting it by rinsing. Then, the scion incision is soaked in a plant growth regulator solution, which helps to stimulate the division and growth of scion cells, enhance its vitality and adaptability, flatten the stock to form a grafting incision, and cut the bottom of the scion into a specific shape and smooth matching, which can increase the contact area between the stock and the scion, facilitate the close combination of the two, promote the alignment of the cambium and the formation of callus tissue, thereby improving the survival rate of the graft and the speed of interface healing, and finally achieving a good fusion of the stock and the scion, so that the grafted plant can grow healthily;

[0033] In S3, the alignment of cambium layers through cut grafting can promote the exchange of substances and information between cells, so that an effective nutrient and water transport channel can be established between the scion and the rootstock;

[0034] In S4, probiotics can improve the microbial community structure around the rootstock and scion, inhibit the growth of harmful microorganisms, enhance the immunity of the plant, and reduce the risk of pests and diseases. At the same time, probiotics can promote the decomposition and transformation of nutrients in the soil, improve soil fertility, enable the rootstock and scion to obtain more sufficient nutrients, and accelerate growth and healing;

[0035] In S5, multi-layer wrapping provides stable physical protection for the grafted joint, effectively reduces the interference and damage of the external environment to the interface, prevents excessive water loss and invasion of pathogens, and the first layer of protective film soaked in probiotic solution can play the beneficial role of probiotics, promote wound healing, enhance plant immunity, and inhibit the growth of harmful microorganisms. The overall wrapping helps to maintain the humidity and temperature of the interface stable, creating suitable conditions for cell division and callus growth, accelerating the fusion of stock and scion, improving the graft survival rate, and ensuring the healthy growth and good development of the grafted plant.

[0036] In S6, the growth environment of the grafted plants is controlled to provide stable conditions for the grafted plants, promote normal cell metabolism and tissue repair, accelerate the healing process of the interface, and untie the outer protective film in time for inspection, which helps to promptly discover possible problems in the healing process, such as infection, poor healing, etc., and take corresponding measures to intervene and adjust, so that the rootstock and scion can be better integrated to form a complete and healthy plant, improve the survival rate and growth quality of the plant, and ultimately achieve the expected goal of grafting.

[0037] In S1, the rootstock includes the roots left after transplanting the seedlings for grafting and the tree roots for direct grafting, and only 1-2 buds on the top of the scion are retained.

[0038] Specifically, the roots after transplanting can quickly take root in the soil by relying on the preliminary root system structure built by the previous growth, and accurately and efficiently absorb water, minerals and other nutrients, thereby improving the early survival rate of grafted seedlings and promoting their vigorous growth. The directly used tree roots can, on the one hand, shorten the seedling raising time, and on the other hand, for fruit trees in the peak production period, if the bark of the main stem of the fruit tree is severely scraped due to rot disease, the roots of the fruit tree will not get nutrition and die. Root grafting and then bridging can prevent the fruit tree from reducing production. At the same time, root grafting can determine the position of the bridge, and retaining 1-2 buds on the top can concentrate the nutrient supply of the scion. Reducing the number of buds can reduce the nutrient consumption of the scion and improve its survival rate after grafting. At the same time, the retained top buds have apical advantage, which can more effectively obtain nutrients and growth hormones, grow quickly and unfold new leaves, accelerate the fusion of the scion and the rootstock, and prompt it to adapt to the new growth environment faster, ultimately improving the overall effect and success rate of grafting.

[0039] The biological fungicide solution in S2 includes carbendazim wettable powder in a ratio of 500-1000 times. The cut of the rootstock left after transplanting the seedlings is soaked in the carbendazim solution for 10-15 minutes. The rootstock for direct grafting is disinfected by flushing with the carbendazim solution for 3-5 minutes. The rootstock is then placed in a well-ventilated place without direct sunlight to dry naturally.

[0040] Specifically, the carbendazim solution can effectively kill the pathogens hidden in the incision of the rootstock, eliminate the risk of infection, and create a clean start for grafting. The soaking time of 10 to 15 minutes can fully exert the bactericidal effect of carbendazim, and the solution can quickly wrap the surface of the tree roots through flushing, powerfully remove the pathogens wrapped in the soil and naturally attached, and reduce the risk of infection. Placing it in a well-ventilated place without direct sunlight to dry naturally can avoid water accumulation in the incision and the growth of pathogens, and at the same time prevent dehydration or damage to the incision caused by direct sunlight, maintain the health and physiological activity of the incision, and facilitate the subsequent smooth combination and efficient healing of the scion, significantly improve the success rate of grafting, and ensure the healthy growth of the grafted plant.

[0041] The plant growth regulator solution in S2 includes 100-200 mg / L indolebutyric acid and 50-100 mg / L naphthaleneacetic acid, and the scion incision is immersed in the plant growth regulator solution for 3-5 minutes.

[0042] Specifically, the main function of indolebutyric acid is to stimulate the division and elongation of plant cells, enhance the plant's ability to absorb nutrients and water, thereby improving the plant's adaptability and growth rate in the new environment, while naphthylacetic acid focuses on promoting cell expansion and elongation. In grafting, naphthylacetic acid helps the growth and differentiation of cells at the interface between the scion and the rootstock, accelerates the healing process, and improves the survival rate of the graft. Through the synergistic effect of indolebutyric acid and naphthylacetic acid, it can stimulate the division and elongation of the scion incision cells, enhance cell vitality, and promote the rapid proliferation of cambium cells, thereby accelerating the formation of callus tissue between the scion and the rootstock, thereby significantly improving the survival rate of the graft and the growth quality of the plant.

[0043] If the thickness of the scion and the rootstock are inconsistent in S3, align the cambium on one side of the scion and the rootstock.

[0044] Specifically, if there is a difference in the thickness of the scion and the rootstock, an effective cell communication and nutrient transfer channel can still be established by aligning the cambium on one side, which can maximize the exchange of substances between cells and promote the formation and growth of callus tissue preferentially on the aligned side, so that the scion can obtain water and nutrient supply from the rootstock.

[0045] The probiotic solution in S4 includes Bacillus subtilis, glucose and acetate buffer.

[0046] Specifically, Bacillus subtilis can improve the microecological environment of the plant rhizosphere, enhance the immunity of the plant, and prevent the occurrence of diseases and insect pests. Glucose provides energy for the growth and metabolism of Bacillus subtilis, promoting its large-scale reproduction and beneficial effects. Acetate buffer helps to maintain the pH stability of the solution, ensuring that Bacillus subtilis survives and functions in a suitable environment. In summary, this probiotic solution can create more favorable growth conditions for grafted plants, accelerate wound healing, and improve the survival rate and growth quality of grafted plants.

[0047] The multi-layer protective film in S5 includes a cellulose film, a polyurethane film and a cotton protective film, and the cellulose film is soaked in the probiotic solution for 5 to 10 minutes.

[0048] Specifically, the first layer is a cellulose film that is in direct contact with the grafting site and is soaked in a probiotic solution. A barrier rich in beneficial bacteria can be formed at the grafting site. Probiotics can inhibit the growth of harmful bacteria and promote wound healing. During the subsequent growth of the plant, the cellulose film can be degraded to avoid damage to the grafting site when the film is torn off, and to improve environmental protection. The second layer is a polyurethane film with certain elasticity and air permeability, which can provide appropriate protection and a good gas exchange environment for the grafting site, and is conducive to cell respiration and metabolism. At the same time, the cotton protective film can increase moisture retention and comfort, and reduce the stimulation and damage of the external environment to the interface. In the subsequent growth stage, the growth of the grafting site can be observed through the transparent cellulose film and polyurethane film by untying the cotton protective film. The synergistic effect of the multi-layer protective film creates a stable, moist, sterile and suitable growth microenvironment for the grafting interface, effectively improving the healing speed and quality of the interface, and ensuring the success of the grafting and the healthy growth of the plant.

[0049] The plant temperature was controlled at 18-28°C after grafting in S6.

[0050] Specifically, temperature control can provide suitable growth conditions for grafted plants. Within the range of 18-28°C, the physiological and metabolic activities of plant cells can proceed normally and efficiently, promoting cell division and tissue differentiation, accelerating the formation and growth of callus tissue at the interface, and thus accelerating the fusion process of rootstock and scion.

[0051] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An environmentally friendly rootstock grafted plant cultivation method, characterized in that: The following steps are included: S1. Select rootstock and scion: Select healthy rootstock and scion without diseases and insect pests, and then prune the scion to be grafted to remove leaves and excess buds; S2. Pretreatment of stock and scion: prepare a biofungicide solution and a plant growth regulator solution, flatten the stock with a knife to form a grafting incision, and soak or rinse the stock incision in a diluted biofungicide solution, then use a sterilized knife to cut the bottom of the scion into a wedge or bevel, the cut surface should be smooth and the length should match the incision of the stock, and soak the incision of the scion in a solution containing a plant growth regulator; S3, grafting operation: insert the processed scion into the flattened cut of the stock, so that the cambium of the scion and the stock are aligned; S4, probiotic symbiotic culture: prepare probiotic solution and use spray method to evenly distribute the probiotics around the rootstock and scion; S5. Multi-layer wrapping: Wrap the grafting site of the rootstock and scion with three layers of protective film, and soak the first layer of protective film in a probiotic solution; S6. Management during the healing period: Control the growth environment of the grafted plants, and untie the outer protective film in time according to the growth conditions of the plants to check the healing status of the interfaces.

2. The environmentally friendly root stock grafted plant cultivation method according to claim 1, characterized in that: The rootstock includes the root left after transplanting the seedlings as the rootstock and the rootstock for direct grafting, and the scion in S1 only retains 1-2 buds on the top.

3. The environmentally friendly root stock grafted plant cultivation method according to claim 1, characterized in that: The biofungicide solution in S2 includes carbendazim wettable powder in a ratio of 500-1000 times liquid. The cut of the rootstock left after transplanting the seedlings is soaked in the carbendazim solution for 10-15 minutes. The rootstock on which the roots are directly grafted is rinsed and disinfected by the carbendazim solution for 3-5 minutes. After that, the rootstock is placed in a well-ventilated place without direct sunlight to dry naturally.

4. The environmentally friendly root stock grafted plant cultivation method according to claim 1, characterized in that: The plant growth regulator solution in S2 includes 100-200 mg / L indolebutyric acid and 50-100 mg / L naphthaleneacetic acid, and the scion incision is immersed in the plant growth regulator solution for 3-5 minutes.

5. The environmentally friendly root stock grafted plant cultivation method according to claim 1, characterized in that: If the thickness of the scion and the stock are inconsistent in S3, the cambium on one side of the scion and the stock is aligned.

6. The environmentally friendly rootstock grafted plant cultivation method according to claim 1, characterized in that: The probiotic solution in S4 includes Bacillus subtilis, glucose and acetate buffer.

7. The environmentally friendly root stock grafted plant cultivation method according to claim 1, characterized in that: The multi-layer protective film in S5 includes a cellulose film, a polyurethane film and a cotton protective film, and the cellulose film is soaked in the probiotic solution for 5 to 10 minutes.

8. The environmentally friendly root stock grafted plant cultivation method according to claim 1, characterized in that: In the S6, the temperature of the plant after grafting is controlled at 18-28°C.

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