Method for inoculating aphids to wheat plants

Abstract

The invention relates to the technical field of agricultural entomology, and discloses a wheat plant aphid inoculation method which comprises the following steps: S1, aphid selection: calculating the number of aphids needing to be inoculated in each potting device according to the number of two aphids in each plant, lightly sweeping and picking the aphids into an EP tube one by one by using a small brush, lightly bouncing the outer wall of the EP tube by using fingernails, and putting the aphids into the EP tube; after ensuring that all aphids are left at the bottom of the pipe, tightly covering the cover, and then starving the aphids for 2 hours; s2, aphid inoculation: uniformly placing EP tubes filled with sufficient aphids into the wheat potting device; s3, counting aphids: after 4 hours, slowly taking back the EP pipe from the wheat potting device, enabling the pipe orifice of the EP pipe to face upwards, carefully counting whether the aphids which do not climb out remain in the EP pipe, and counting the number of the aphids. According to the method, the aphids are inoculated through the EP tube, active colonization of the aphids is achieved, it is guaranteed that the initial number of the aphids is consistent, and it is guaranteed that the aphids have high activity initially.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural entomology, in particular to a method for inoculating aphids into wheat plants. Background Art

[0002] The main diseases and pests that harm wheat production in Sichuan are "three diseases and two insects", namely wheat fusarium head blight, stripe rust, powdery mildew, aphids, and wheat spiders. Among them, aphids are the largest pest of wheat. Aphids use piercing-sucking mouthparts to suck wheat sap, and the honeydew they secrete affects wheat photosynthesis and respiration, and even spreads barley yellow dwarf virus, causing wheat yield reduction, quality decline, and thus reduced production efficiency. When wheat starts to form ears, winged aphids begin to colonize, reproduce, and harm wheat.

[0003] Due to climate change, changes in farming systems and other reasons, the damage caused by wheat aphids has been increasing year by year. Breeding wheat aphid-resistant varieties and improving wheat's own resistance to aphids are the fundamental ways to prevent and control wheat aphids. Accurate evaluation and identification of wheat's resistance to aphids is crucial.

[0004] There are two main methods for evaluating wheat resistance to aphids, namely the natural aphid infestation method in the field and the indoor potted net inoculation method. Both evaluation methods include an aphid quantity ratio parameter based on the insect population. The natural aphid infestation method in the field has the following main shortcomings: 1. It requires aphid resistance data from multiple locations for many years, which is a huge workload; 2. This method is greatly affected by the environment. In case of strong winds and heavy rains, the aphid quantity drops sharply and the results are lost; 3. Aphids feed freely in the field, and the plants affect each other, resulting in aphids' non-specific feeding. The results cannot accurately explain the damage of aphids to the plant (wheat), that is, the resistance of wheat to aphids, thus affecting the evaluation results; 4. Aphids are prone to form central aphid plants when feeding and reproducing in the field. This clustering situation cannot be used as a resistance identification result. The indoor potted net inoculation method can effectively avoid the above four shortcomings, which is conducive to aphids feeding exclusively in a specific material, and the insect population of aphids is accurately affected by the wheat's own resistance, so the resistance identification results are more accurate and reliable. The existing indoor potted plant aphid inoculation method (artificial inoculation) has only one step, which is to directly use a small brush / brush to gently sweep up the aphids in a dry and fluffy state, and gently place the aphids one by one on the wheat leaves, which cannot ensure the consistency of the number of aphids. When using a brush or brush to pick up aphids and release aphids, a small number of aphids often fall off during the process, affecting the number of insects; resulting in inconsistent feeding and damage effects of aphids on wheat plants after inoculation, thereby affecting the accuracy of the final anti-aphid evaluation; the consistency of aphid vitality cannot be guaranteed. When using a brush or brush to pick up aphids and release aphids, a small number of aphids are pricked by the bristles during the process, resulting in weak vitality or death, which affects the activity of aphids and directly leads to insufficient number of aphids actually inoculated; the number of colonized aphids cannot be guaranteed to be accurate. After placing aphids on wheat leaves or leaf axils, they often fall off due to leaf shaking or the sudden change of the environment from the brush to the leaf. The fallen aphids either die or crawl back onto the plant, resulting in inaccurate counts of the initial colonization of aphids.

[0005] To this end, we proposed a method of inoculating wheat plants with aphids to solve the problem. Summary of the invention

[0006] 1. Technical issues to be resolved In view of the deficiencies of the prior art, the present invention provides a method for inoculating aphids on wheat plants, which solves the problems in the above-mentioned background technology.

[0007] (II) Technical solution To achieve the above object, the present invention provides the following technical solution: a method for inoculating aphids on wheat plants, comprising the following steps: S1: Select aphids Calculate the number of aphids that need to be inoculated in each potted device based on the amount of 2 aphids per plant, use a small brush to gently sweep the aphids one by one into the EP tube, flick the outer wall of the EP tube with your fingernails to ensure that all aphids remain at the bottom of the tube, close the lid tightly, and then starve the aphids for 2 hours; S2: First aphid supplementation uniformly placing EP tubes containing sufficient aphids into the wheat pot plant device; S3: Count aphids After 4 hours, slowly take the EP tube back from the wheat potting device, with the tube mouth facing upwards, carefully count whether there are any aphids left in the EP tube and count the number of aphids; S4: Second aphid supplementation Continue to select adult aphids with good vitality and uniform body size, pick them up one by one, and gently graft them into the axils of wheat leaves until the number of aphids is sufficient: 2 per plant; to ensure that each wheat plant has 2 colonized aphids; during this period, pay attention to replace the brush to prevent the brush hair from being wetted and adhered by moist liquids such as honeydew, and continued operation will threaten the vitality of the aphids or even kill them; S5: closing the device to complete the entire aphid inoculation process.

[0008] Preferably, the aphids in step S1 are selected from adult aphids with better vitality and more uniform body shape.

[0009] Preferably, the specification of the EP tube is 2.0 ml.

[0010] Preferably, the specific method of step S2 is: lightly tap the upper part of the outer wall of the EP tube with a finger, and after the aphids are fully concentrated at the bottom of the tube, open the EP tube cover, carefully and horizontally place the EP tube in the wheat potted device, with the tube cover facing one side and the tube mouth facing the connection between the root and the stem of the wheat plant, so that the hungry aphids can crawl onto the small surface plant by themselves to quickly feed and complete colonization.

[0011] Preferably, the specification of the small brush is 1.2 cm.

[0012] (III) Beneficial effects Compared with the prior art, the present invention provides a method for inoculating aphids in wheat plants, which has the following beneficial effects: 1. The present invention utilizes EP tubes to inoculate aphids to realize active colonization of aphids, thereby ensuring that the initial population of aphids is consistent and that the initial activity of aphids is relatively high.

[0013] 2. The present invention can achieve rapid and short-term completion of aphid inoculation. This includes that multiple people can simultaneously perform aphid selection operations, placing aphids in EP tubes, i.e., S1; and one person can perform aphid collection operations, i.e., S2. This shortens the working time, unifies the specific operations (especially S2), reduces the impact of human operation factors, and ensures more reliable results.

[0014] 3. The present invention can more accurately evaluate the resistance of wheat to aphids, thereby improving the resistance level of wheat varieties, which is beneficial to large-scale wheat production and reducing aphid damage. DETAILED DESCRIPTION

[0015] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0016] A method for inoculating wheat plants with aphids comprises the following steps: S1: Select aphids Calculate the number of aphids that need to be inoculated in each potted device based on the amount of 2 aphids per plant. Here, select adult aphids with good vitality and uniform body size. Use a small brush with a specification of 1.2 cm to gently sweep the aphids one by one into a 2.0 ml EP tube. Use fingernails to flick the outer wall of the EP tube to ensure that all aphids remain at the bottom of the tube, then close the lid tightly and starve the aphids for 2 hours; S2: First aphid supplementation The EP tubes filled with sufficient aphids are uniformly placed in the wheat potted plant device. The specific method is: flick the upper part of the outer wall of the EP tube with a finger, and after the aphids are fully concentrated at the bottom of the tube, open the tube cover of the EP tube, carefully and horizontally place the EP tube in the wheat potted plant device, with the tube cover facing one side and the tube mouth facing the connection between the root and the stem of the wheat plant, so that the hungry aphids can crawl to the small surface plant by themselves to quickly feed and complete colonization; S3: Count aphids After 4 hours, slowly take the EP tube back from the wheat potting device, with the tube mouth facing upwards, carefully count whether there are any aphids left in the EP tube and count the number of aphids; S4: Second aphid supplementation Continue to select adult aphids with good vitality and uniform body size, pick them up one by one, and gently graft them into the axils of wheat leaves until the number of aphids is sufficient: 2 per plant; to ensure that each wheat plant has 2 colonized aphids; during this period, pay attention to replace the brush to prevent the brush hair from being wetted and adhered by moist liquids such as honeydew, and continued operation will threaten the vitality of the aphids or even kill them; S5: Use 80-mesh nylon mesh to close the device and complete the entire aphid inoculation process.

[0017] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for inoculating wheat plants with aphids, characterized in that: The following steps are involved: S1: Select aphids Calculate the number of aphids that need to be inoculated in each potted device based on the amount of 2 aphids per plant, use a small brush to gently sweep the aphids one by one into the EP tube, flick the outer wall of the EP tube with your fingernails to ensure that all aphids remain at the bottom of the tube, close the lid tightly, and then starve the aphids for 2 hours; S2: Aphids uniformly placing EP tubes containing sufficient aphids into the wheat pot plant device; S3: Count aphids After 4 hours, slowly take the EP tube back from the wheat potting device, with the tube mouth facing upwards, carefully count whether there are any aphids left in the EP tube and count the number of aphids; S4: Aphid supplement Continue to select adult aphids with good vitality and uniform body shape, pick them up one by one with a small brush, and gently graft them into the leaf axils of wheat until the number of aphids is sufficient: 2 heads / plant; to ensure that the number of aphids colonizing each wheat plant is 2 heads; during this period, pay attention to replace the brush to prevent the brush hair from being wetted and adhered by moist liquids such as honeydew, and continuing the operation will threaten the vitality of the aphids or even kill them; S5: closing the device to complete the entire aphid inoculation process.

2. A method for inoculating wheat plants with aphids according to claim 1, characterized in that: In step S1, adult aphids with better vitality and more uniform body shape are selected.

3. A method for inoculating wheat plants with aphids according to claim 1, characterized in that: The specification of EP tube is 2.0ml.

4. A method for inoculating wheat plants with aphids according to claim 1, characterized in that: The specific method of step S2 is: lightly tap the upper part of the outer wall of the EP tube with your fingers, and after the aphids are fully concentrated at the bottom of the tube, open the EP tube cover, carefully place the EP tube horizontally in the wheat potted device, with the tube cover facing one side and the tube mouth facing the connection between the root and the stem of the wheat plant, so that the hungry aphids can crawl onto the wheat plant by themselves to quickly feed and complete colonization.

5. A method for inoculating wheat plants with aphids according to claim 1, characterized in that: The specification of the small brush is 1.2cm.