Application of bacillus velezensis TCS001 to improvement of broccoli growth traits

Abstract

According to the application of the bacillus velezensis TCS001 as the medicine for improving the growth traits of the cauliflower, in the germination period, the seedling period, the rosette period, the heading period and the harvesting period of the cauliflower, after 450-fold root irrigation and spraying treatment are carried out by adopting a bacillus velezensis TCS001 suspending agent, compared with a control group, the heading rate is improved by 80.4%, the average heading diameter is increased by 5.25%, and the growth traits of the cauliflower are improved. The yield of the cauliflower fruits is obviously improved.

Description

Technical Field

[0001] The invention relates to the field of using Bacillus Velez subtilis as a plant growth regulator, and in particular to application of Bacillus Velez subtilis TCS001 in regulating the growth traits of cauliflower. Background Art

[0002] Bacillus velezensis is a new type of biocontrol bacteria. In 2005, Spanish scholars Ruiz-García et al. isolated two strains, CR-14b and CR-502T, which can synthesize a large amount of lipopeptides and have strong antibacterial activity. Bacillus velezensis is widely distributed and can survive in adverse conditions such as drought and salinity. There are few related products, and the development prospects are broad.

[0003] Bacillus velezensis TCS001 was isolated from Bohai silt. The original strain was marine Bacillus CT2628. After mutagenesis and stabilization, it was identified and named Bacillus velezensis TCS001. It has been deposited in the General Microbiological Center of China Microbiological Culture Collection Administration (CGMCC) with the deposit number CGMCC No.8921, and was first published in patent ZL201410168402.2. After growing on NA medium, the single colony morphology of TCS001 is nearly round, light yellow, and opaque; the surface of the colony is smooth and the edges are neat in the early stage of culture, and the surface is wrinkled in the later stage, the edges are slightly irregular, there is a bulge in the middle, and it spreads in a cloud-like manner around. Gram staining showed that the Velez Bacillus TCS001 strain was Gram-positive and rod-shaped; it had a certain inhibitory effect on cucumber gray mold, cucumber vine blight, cucumber sclerotinia, cucumber brown spot, cotton wilt and banana leaf spot, among which the inhibition rate against cucumber gray mold was the highest, reaching 87.66%.

[0004] It has been reported in the literature that 7500 times diluted 1.5% Verticillium dahliae activated protein wettable powder can effectively increase the heading rate of cauliflower by 27.08% and the average ball diameter by 10.19%, but it was found that the higher the application concentration, the smaller the average single ball diameter; 1000 times diluted 5% amino oligosaccharide AS can increase the heading rate of cauliflower by 25.29% and the average ball diameter by 4.95%; 500 times diluted 6% oligosaccharide·chain protein WP can increase the heading rate of cauliflower by 2.30% and the average ball diameter by 7.07%.

[0005] Currently, there is no report on the application of Bacillus Velez TCS001 on cauliflower. Summary of the invention

[0006] The main purpose of the present invention is to provide application of Bacillus Velez TCS001 as a drug for improving the growth traits of cauliflower.

[0007] Another main object of the present invention is to provide a method for improving the growth traits of cauliflower using Bacillus Velez TCS001.

[0008] Preferably, the improving the growth traits of cauliflower includes increasing the total number of cauliflower heads, the head setting rate and the average head diameter.

[0009] Preferably, the Bacillus Velez subtilis TCS001 is a fermentation broth of Bacillus Velez subtilis TCS001.

[0010] Preferably, the Bacillus Velez TCS001 fermentation broth is prepared as a suspension.

[0011] Preferably, the formula of the suspension concentrate includes 40% of Bacillus Velez TCS001 fermentation broth, 3% of dispersant SXC, 3% of dispersant UNA, 0.15% of thickener xanthan gum, 0.2% of preservative kason, 0.2% of preservative sodium benzoate, and 53.45% of water.

[0012] Preferably, the application concentration of the suspension is 300-700 times diluted.

[0013] Preferably, the application concentration of the suspension is 450 times diluted.

[0014] Preferably, the Bacillus Velez subtilis TCS001 is applied by root irrigation and / or spraying.

[0015] Preferably, the application time of the Bacillus Velez TCS001 is one or more of the flower bud differentiation stage, the initial flowering stage, and the full flowering stage of cauliflower;

[0016] Preferably, the Bacillus Velez TCS001 is applied during the germination stage, seedling stage, rosette stage, heading stage and harvest stage, once a week until the end of harvest.

[0017] Preferably, the Bacillus Velez TCS001 is applied once within 5 days after harvesting.

[0018] Beneficial technical effects of the present invention

[0019] During the germination, seedling, rosette, heading and harvesting stages of cauliflower, after root irrigation and spraying with 450 times the suspension concentrate of Bacillus Velez subtilis TCS001, the heading rate increased by 80.4% and the average head diameter increased by 5.25% compared with the control group.

[0020] Treatment with Bacillus Velez TCS001 can significantly increase cauliflower fruit yield.

[0021] During the experiment, it was observed that the size and volume of the cauliflower heads and other traits were significantly better than those of the blank control after the cauliflower was treated with Bacillus Velez TCS001. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 Cauliflower after control and Bacillus Velez TCS001 shed treatment; Specific embodiments

[0023] Materials and Methods

[0024] Test materials

[0025] The test bacterial agent of the present invention is the suspension concentrate of Bacillus Velezii TCS001 (3.9×10 9 CFU / mL), the specific formula is: TCS001 fermentation broth 40%, dispersant SXC 3%, dispersant UNA 3%, thickener xanthan gum 0.15%, preservative kason 0.2%, preservative sodium benzoate 0.2%, water 53.45%.

[0026] Test plants

[0027] Experimental variety: cauliflower.

[0028] Main instruments:

[0029] ME104E electronic balance (Sartorius Scientific Instruments Co., Ltd.)

[0030] Test method:

[0031] See Table 1 for treatment methods

[0032] Table 1 Treatment methods of cauliflower test with Bacillus Velez TCS001 suspension concentrate

[0033]

[0034] The suspension concentrate of Bacillus Velez TCS001 was diluted with water to the corresponding concentration and then used for root irrigation and spraying treatments.

[0035] Spray treatment: Spray cauliflower once at the seedling stage, rosette stage, heading stage and harvest stage respectively. Spray continuously from the heading stage to the harvest stage once a week.

[0036] Determination of Cauliflower Yield Index

[0037] When cauliflower entered the harvest period, the plants affected by the edge effect were excluded from each plot and the remaining plants were harvested in batches by plot, and the number of heads in each treatment was recorded, and the head rate was calculated according to the following formula. Five randomly marked cauliflowers were selected from each plot and washed with distilled water, the surface moisture was absorbed with filter paper, the head diameter was measured with a tape measure, and the average head diameter of different treatments was calculated.

[0038] Heading rate = (number of head-forming plants / total number of plants) × 100%

[0039] As shown in Table 2, the application of Bacillus Velez TCS001 suspension diluted 450 times for root irrigation spray treatment, once after transplanting, once a week from seedling stage to harvest stage, for a total of 4 times, can increase the cauliflower head rate by 80.4%, increase the average head diameter by 5.25%, and greatly increase the cauliflower yield.

[0040] Table 2 Cauliflower growth characteristics test results

[0041]

[0042] During the experiment, it was observed that Bacillus Velez TCS001 had a positive effect on the size, number of heads, glossiness and other traits of cauliflower, greatly increasing the yield of cauliflower.

[0043] The specific implementation methods described above further illustrate the objectives, technical solutions and beneficial effects of the present invention in detail. It should be understood that the above description is only a specific implementation method of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. Application of Bacillus Velez TCS001 as a drug for improving the growth traits of cauliflower.

2. The use according to claim 1, characterized in that: The cauliflower growth traits include total head number, head setting rate, average head diameter and the like.

3. A method for improving the growth characteristics of cauliflower using Bacillus Velez TCS001, characterized in that: The method of improving the growth traits of cauliflower includes increasing the total number of cauliflower heads, increasing the head setting rate and / or the average head diameter of cauliflower heads, etc.

4. The use according to any one of claims 1 to 2 or the method according to claim 3, characterized in that: The Bacillus Velez TCS001 is the fermentation liquid of Bacillus Velez TCS001.

5. The use according to claim 4 or the method according to claim 4, characterized in that: The Bacillus Velez TCS001 fermentation liquid is prepared into a suspension.

6. The use according to claim 5 or the method according to claim 5, characterized in that: The suspending agent comprises 40% of Bacillus Velez TCS001 fermentation liquid, 3% of dispersant SXC, 3% of dispersant UNA, 0.15% of thickener xanthan gum, 0.2% of preservative kason, 0.2% of preservative sodium benzoate and 53.45% of water.

7. The use according to claim 6 or the method according to claim 6, characterized in that: The application concentration of the suspension is 300-700 times diluted; preferably, the application concentration is 450 times diluted.

8. The use according to any one of claims 1 to 2 or the method according to claim 3, characterized in that: The application time of the Bacillus Velez TCS001 can be one or more of the germination stage, seedling stage, rosette stage, heading stage and harvest stage.

9. The use according to any one of claims 1 to 2 or the method according to claim 3, characterized in that: The application method of the Bacillus Velez TCS001 is root irrigation and / or spraying treatment.

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