Eurotium cristatum fermented cavaleriei delavayi tea and preparation method thereof

Abstract

The invention relates to eurotium cristatum fermented cavaleriei delavayi tea and a preparation method thereof, and belongs to the technical field of fermented tea leaves. The preparation method of the eurotium cristatum fermented jute tea comprises the following steps: mixing the jute tea with a complex microbial inoculant, and performing primary fermentation to obtain a tea sample; and mixing the tea sample with the Jute tea extract, and carrying out secondary fermentation, filtration and sterilization to obtain the Jute tea. The eurotium cristatum fermented boea cavaleriei tea soup prepared by the preparation method disclosed by the invention has excellent oxidation resistance.

Description

Technical Field

[0001] The invention belongs to the technical field of fermented tea leaves and relates to golden flower fermented long capsule jute tea and a preparation method thereof. Background Art

[0002] The medicinal value of long-capsule jute is extremely high. Its leaves are rich in antioxidant ingredients such as flavonoids and polyphenols. At the same time, it also has rich nutritional value, containing a variety of sugars, dietary fiber, minerals (such as calcium, potassium, sodium, iron, selenium) and vitamin E, vitamin C and β-carotene, as well as seven essential amino acids for the human body. The root of jute tastes light and slightly bitter, and is mild in nature. It is used to treat diarrhea, dysentery, promote diuresis and relieve stranguria; it has the effect of stopping bleeding and stopping diarrhea. Jute leaves also taste light and slightly bitter, and are mild in nature. They have the effect of draining pus and promoting muscle growth. They are suitable for treating sores, gas syndrome, heartache, abdominal pain and abdominal mass.

[0003] The tea made from the fresh leaves of Cortex Psoraleae produced by traditional technology is light yellow in color, with a green and astringent taste and a poor taste, which limits people's preference for this tea and also hinders the performance of its health function. Through the application of Jinhua fermentation technology, the unique nutrition and health-care ingredients of Cortex Psoraleae tea can be retained to the greatest extent, while giving it the color, aroma, taste and shape of fermented tea, making it unique in flavor and better in taste.

[0004] Golden Flower Fungus is a kind of fungus belonging to the genus Eurotium of the family Mycorrhizaceae. Studies have found that Golden Flower Fungus can improve the quality and active ingredients of tea during the fermentation process, help lower blood sugar and blood lipids, reduce the risk of atherosclerosis, and has an antioxidant effect, which is beneficial to gastrointestinal health. Adding Golden Flower Fungus during the fermentation process of tea can produce beneficial substances such as protein, improve the quality of the fermented product, reduce the astringency and bitterness of the tea, and increase the content of active ingredients in the tea, showing a wide range of application prospects and research value. Summary of the invention

[0005] The purpose of the present invention is to provide a golden flower fermented long capsule jute tea and a preparation method thereof. The golden flower fermented long capsule jute tea soup prepared by the present invention has excellent antioxidant performance.

[0006] The purpose of the present invention can be achieved through the following technical solutions:

[0007] A method for preparing golden flower fermented long capsule jute tea comprises the following steps:

[0008] Step 1, mixing the long capsule jute tea and the composite bacterial agent and fermenting them once to obtain a tea sample;

[0009] Step 2: Mix the tea sample with the extract of Cordata longifolia tea and perform secondary fermentation, filter and sterilize to obtain the product.

[0010] As a preferred technical solution of the present invention, in step 1, the primary fermentation is carried out at a temperature of 25-30°C for 2-3 days; and the volume ratio of the composite bacterial agent to the long capsule jute tea is 3-4:10.

[0011] As a preferred technical solution of the present invention, in step 2, the secondary fermentation is fermented for 2-3 days under continuous illumination at a temperature of 25-30°C and a shaking speed of 120-150r / min; the dosage ratio of the tea sample and the long capsule jute tea extract is 2.5-3.0g:110-120mL.

[0012] As a preferred technical solution of the present invention, the method for preparing the long capsule jute tea extract comprises the following steps:

[0013] The long capsule jute tea leaves are washed, dried, ground and sieved to obtain long capsule jute tea powder, and a 60wt% ethanol aqueous solution is added for high temperature extraction, filtered, and after extraction three times, the extracts are combined to obtain a long capsule jute tea extract.

[0014] As a preferred technical solution of the present invention, in the preparation of the long capsule jute tea extract, the sieving is through a 40-mesh sieve; the high temperature extraction is extraction at a temperature of 78-82°C for 20-30 minutes; the dosage ratio of the long capsule jute tea powder and distilled water is 9-10g:100-110mL; the moisture content of the long capsule jute tea powder is 6-8%.

[0015] As a preferred technical solution of the present invention, the preparation method of the composite bacterial agent comprises the following steps:

[0016] The nanocellulose and cinnamon essential oil are ultrasonically treated in anhydrous ethanol, baking soda and mixed monomers are added and stirred, and after rotary evaporation, a hydrochloric acid solution is added and continued to be stirred, and then filtered, washed and vacuum dried to obtain a carrier, and the carrier is placed in a golden flower fungus suspension, stirred, and allowed to stand to obtain a composite bacterial agent.

[0017] As a preferred technical solution of the present invention, the ultrasonic treatment is ultrasonic treatment at a frequency of 200-300W for 40-60min; the stirring and mixing is stirring at a temperature of 24-28°C for 6-7h.

[0018] As a preferred technical solution of the present invention, the continued stirring is stirring at a speed of 500-800 rpm for 45-60 minutes; the washing is washing three times with deionized water; the vacuum drying is vacuum drying at a temperature of 60°C to constant weight; the stirring treatment is stirring at a speed of 150-200 rpm for 30-40 minutes; and the standing time is 1.5-2.0 hours.

[0019] As a preferred technical solution of the present invention, the mass ratio of the nanocellulose, cinnamon essential oil, anhydrous ethanol, baking soda, mixed monomer and hydrochloric acid solution is 12-14: 1.8-2.0: 30-40: 0.04-0.06: 2.3-2.8: 40-50; the mass fraction of the hydrochloric acid solution is 3%.

[0020] As a preferred technical solution of the present invention, the mixed monomer is formed by mixing phenylalanine and cysteine ​​in a mass ratio of 1.0-1.2:2.5-3.0.

[0021] As a preferred technical solution of the present invention, the usage ratio of the carrier and the golden flower fungus suspension is 1.0-1.3 g: 30-32 mL; the concentration of the golden flower fungus suspension is 1.2×10 8 -2.0×10 8 CFU / mL; the golden flower fungus is the coronoid fungus, numbered CGMCC10610.

[0022] The invention discloses golden flower fermented long capsule jute tea prepared by the preparation method.

[0023] Beneficial effects of the present invention:

[0024] The present invention forms a composite carrier with an interpenetrating network structure by compounding nanocellulose, cinnamon essential oil, phenylalanine and cysteine, so that the cross-linking groups are more abundant, the pore structure and specific surface area of ​​the composite carrier are increased, and the pore structure of the carrier is further enriched by combining with baking soda to expand the pores, thereby improving the immobilization effect on golden flower fungi and making the thermal stability and pH tolerance of the composite carrier stronger. The skeleton of the cellulose contains a large number of hydroxyl groups, and the carboxyl groups of phenylalanine and cysteine ​​promote the interaction with the cinnamon essential oil through hydrogen bonds, thereby increasing the stability of the porous carrier and forming an interaction force with the golden flower fungi, thereby making the combination between the composite carrier and the golden flower fungi more closely. The amino group and the thiol group introduced into the carrier can also complex metal ions, thereby protecting the golden flower fungi from the inhibition of metal ions, improving the activity of the golden flower fungi, promoting the extraction of the active components of the long capsule jute tea and increasing the stability thereof. DETAILED DESCRIPTION

[0025] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with the embodiments.

[0026] Example 1

[0027] A method for preparing golden flower fermented long capsule jute tea comprises the following steps:

[0028] Step 1: After mixing the long capsule jute tea and the composite bacterial agent, ferment at 25° C. for 2 days to obtain a tea sample; the volume ratio of the composite bacterial agent to the long capsule jute tea is 3:10;

[0029] Step 2: Mix the tea sample with the extract of Cordata longifolia tea, ferment for 2 days under continuous illumination at 25°C and a shaking speed of 120 r / min, filter, and sterilize to obtain the tea; the usage ratio of the tea sample to the extract of Cordata longifolia tea is 2.5 g:110 mL.

[0030] The preparation method of the long capsule jute tea extract comprises the following steps:

[0031] The long capsule jute tea leaves are washed, dried, ground, and passed through a 40-mesh sieve to obtain long capsule jute tea powder, and a 60wt% ethanol aqueous solution is added, extracted at 78°C for 20 minutes, filtered, and extracted three times, and the extracts are combined to obtain a long capsule jute tea extract; the usage ratio of the long capsule jute tea powder and distilled water is 9g:100mL; the moisture content of the long capsule jute tea powder is 6%.

[0032] The preparation method of the composite bacterial agent comprises the following steps:

[0033] The nanocellulose and cinnamon essential oil were placed in anhydrous ethanol and ultrasonicated at a frequency of 200W for 40 minutes, and then baking soda and mixed monomers were added and stirred at 24°C for 6 hours. After rotary evaporation, hydrochloric acid solution was added and stirred at a speed of 500rpm for 45 minutes, filtered, washed three times with deionized water, and vacuum dried at 60°C to constant weight to obtain a carrier. The carrier was placed in a suspension of golden flower fungus and stirred at a speed of 150rpm for 30 minutes, and then allowed to stand for 1.5 hours to obtain a composite bacterial agent; wherein the mass ratio of the nanocellulose, cinnamon essential oil, anhydrous ethanol, baking soda, mixed monomer and hydrochloric acid solution is 12:1.8:30:0.04:2.3:40; the mass fraction of the hydrochloric acid solution is 3%; the mixed monomer is prepared by mixing phenylalanine and cysteine ​​in a mass ratio of 1.0:2.5; the dosage ratio of the carrier and the golden flower fungus suspension is 1.0g:30mL; the concentration of the golden flower fungus suspension is 1.2×10 8 CFU / mL.

[0034] Example 2

[0035] A method for preparing golden flower fermented long capsule jute tea comprises the following steps:

[0036] Step 1: After mixing the long capsule jute tea and the composite bacterial agent, ferment at 28° C. for 2.5 days to obtain a tea sample; the volume ratio of the composite bacterial agent to the long capsule jute tea is 3.5:10;

[0037] Step 2: Mix the tea sample with the Cordyceps sinensis tea extract, ferment for 2.5 days under continuous illumination at 28°C and a shaking speed of 140 r / min, filter, and sterilize to obtain the tea; the usage ratio of the tea sample to the Cordyceps sinensis tea extract is 2.8g:115mL.

[0038] The preparation method of the long capsule jute tea extract comprises the following steps:

[0039] The long capsule jute tea leaves are washed, dried, ground, and passed through a 40-mesh sieve to obtain long capsule jute tea powder, and a 60wt% ethanol aqueous solution is added, extracted at 80°C for 25 minutes, filtered, and extracted three times, and the extracts are combined to obtain a long capsule jute tea extract; the usage ratio of the long capsule jute tea powder and distilled water is 9.5g:105mL; the moisture content of the long capsule jute tea powder is 7%.

[0040] The preparation method of the composite bacterial agent comprises the following steps:

[0041] The nanocellulose and cinnamon essential oil were placed in anhydrous ethanol and ultrasonicated at a frequency of 250W for 50 minutes, and then baking soda and mixed monomers were added and stirred at 26°C for 6.5 hours. After rotary evaporation, hydrochloric acid solution was added and stirred at a speed of 650rpm for 52 minutes, filtered, washed three times with deionized water, and vacuum dried at 60°C to constant weight to obtain a carrier. The carrier was placed in a golden flower fungus suspension and stirred at a speed of 175rpm for 35 minutes, and then allowed to stand for 1.8 hours to obtain a composite bacterial agent; wherein the mass ratio of the nanocellulose, cinnamon essential oil, anhydrous ethanol, baking soda, mixed monomer and hydrochloric acid solution is 13:1.9:35:0.05:2.6:45; the mass fraction of the hydrochloric acid solution is 3%; the mixed monomer is composed of phenylalanine and cysteine ​​mixed in a mass ratio of 1.1:2.8; the dosage ratio of the carrier and the golden flower fungus suspension is 1.2g:31mL; the concentration of the golden flower fungus suspension is 1.6×10 8 CFU / mL.

[0042] Example 3

[0043] A method for preparing golden flower fermented long capsule jute tea comprises the following steps:

[0044] Step 1: After mixing the long capsule jute tea and the composite bacterial agent, ferment at 30° C. for 3 days to obtain a tea sample; the volume ratio of the composite bacterial agent to the long capsule jute tea is 4:10;

[0045] Step 2: Mix the tea sample with the extract of Cordata longifolia tea, ferment for 3 days under continuous illumination at 30°C and a shaking speed of 150 r / min, filter, and sterilize to obtain the tea; the usage ratio of the tea sample to the extract of Cordata longifolia tea is 3.0 g:120 mL.

[0046] The preparation method of the long capsule jute tea extract comprises the following steps:

[0047] The long capsule jute tea leaves are washed, dried, ground, and passed through a 40-mesh sieve to obtain long capsule jute tea powder, and a 60wt% ethanol aqueous solution is added, extracted at 82°C for 30 minutes, filtered, and extracted three times, and the extracts are combined to obtain a long capsule jute tea extract; the usage ratio of the long capsule jute tea powder and distilled water is 10g:110mL; the moisture content of the long capsule jute tea powder is 8%.

[0048] The preparation method of the composite bacterial agent comprises the following steps:

[0049] The nanocellulose and cinnamon essential oil were placed in anhydrous ethanol and ultrasonicated at a frequency of 300W for 60 minutes, and then baking soda and mixed monomers were added and stirred at 28°C for 7 hours. After rotary evaporation, hydrochloric acid solution was added and stirred at 800rpm for 60 minutes, filtered, washed three times with deionized water, and vacuum dried at 60°C to constant weight to obtain a carrier. The carrier was placed in a suspension of golden flower fungus and stirred at 200rpm for 40 minutes, and then stood for 2.0 hours to obtain a composite bacterial agent; wherein the mass ratio of the nanocellulose, cinnamon essential oil, anhydrous ethanol, baking soda, mixed monomer and hydrochloric acid solution is 14:2.0:40:0.06:2.8:50; the mass fraction of the hydrochloric acid solution is 3%; the mixed monomer is prepared by mixing phenylalanine and cysteine ​​in a mass ratio of 1.2:3.0; the dosage ratio of the carrier and the golden flower fungus suspension is 1.3g:32mL; the concentration of the golden flower fungus suspension is 2.0×10 8 CFU / mL.

[0050] Comparative Example 1

[0051] Compared with Example 3, the difference in Comparative Example 1 is that nanocellulose is not used, and the other components, preparation steps and parameters are the same.

[0052] Comparative Example 2

[0053] Compared with Example 3, Comparative Example 2 is different in that cinnamon essential oil is not used, and the remaining components, preparation steps and parameters are the same.

[0054] Comparative Example 3

[0055] Compared with Example 3, the difference in Comparative Example 3 is that the mixed monomer is cysteine, and the other components, preparation steps and parameters are the same.

[0056] Comparative Example 4

[0057] Compared with Example 3, Comparative Example 4 is different in that the mixed monomer is phenylalanine, and the other components, preparation steps and parameters are the same.

[0058] Comparative Example 5

[0059] Compared with Example 3, Comparative Example 5 is different in that no mixed monomer is used, and the remaining components, preparation steps and parameters are the same.

[0060] Comparative Example 6

[0061] Compared with Example 3, the difference in Comparative Example 6 is that step 2;

[0062] Step 2: Mix the tea sample with the long capsule jute tea extract, and ferment at 30°C and 150r / min shaking speed for 3 days; the amount ratio of the tea sample to the long capsule jute tea extract is 3.0g:120mL;

[0063] The remaining components, preparation steps and parameters were the same.

[0064] The samples prepared in Examples 1-3 and Comparative Examples 1-6 were subjected to the following performance tests, respectively, and the test results are shown in Table 1.

[0065] DPPH scavenging ability test: 4.0 mL of DPPH solution and the sample to be tested (Examples 1-3, Comparative Examples 1-6) were added to a 10 mL colorimetric tube in sequence, and then anhydrous ethanol was added to the scale. After mixing, the absorbance was immediately measured at a wavelength of 517 nm using a 1 cm colorimetric dish, and the absorbance was recorded as A1. After being placed at room temperature and protected from light for 30 minutes, the absorbance was measured and recorded as A2. The blank control was only added with DPPH ethanol solution, and its absorbance was recorded as A0.

[0066] The calculation method of DPPH clearance rate is: DPPH clearance rate = [1-(A1-A2) / A0] × 100%.

[0067] Table 1

[0068] DPPH clearance rate (%) Example 1 96.4 Example 2 96.8 Example 3 97.1 Comparative Example 1 83.5 Comparative Example 2 80.3 Comparative Example 3 83.4 Comparative Example 4 84.2 Comparative Example 5 80.8 Comparative Example 6 84.6

[0069] From the test results in Table 1, it can be seen that compared with Comparative Examples 1-6, the golden flower fermented long capsule jute tea soup prepared by the present invention has excellent antioxidant properties.

[0070] The present invention forms a composite carrier with an interpenetrating network structure by compounding nanocellulose, cinnamon essential oil, phenylalanine and cysteine, so that the cross-linking groups are more abundant, the pore structure and specific surface area of ​​the composite carrier are increased, and the pore structure of the carrier is further enriched by combining with baking soda to expand the pores, thereby improving the immobilization effect on golden flower fungi and making the thermal stability and pH tolerance of the composite carrier stronger. The skeleton of the cellulose contains a large number of hydroxyl groups, and the carboxyl groups of phenylalanine and cysteine ​​promote the interaction with the cinnamon essential oil through hydrogen bonds, thereby increasing the stability of the porous carrier and forming an interaction force with the golden flower fungi, thereby making the combination between the composite carrier and the golden flower fungi more closely. The amino group and the thiol group introduced into the carrier can also complex metal ions, thereby protecting the golden flower fungi from the inhibition of metal ions, improving the activity of the golden flower fungi, promoting the extraction of the active components of the long capsule jute tea and increasing the stability thereof.

[0071] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any indirect modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A method for preparing golden flower fermented long capsule jute tea, characterized in that: The preparation method comprises the following steps: Step 1, mixing the long capsule jute tea and the composite bacterial agent and fermenting them once to obtain a tea sample; Step 2, mixing the tea sample with the long capsule jute tea extract and performing secondary fermentation, filtering, and sterilizing to obtain; The preparation method of the composite bacterial agent comprises the following steps: The nanocellulose and cinnamon essential oil are ultrasonically treated in anhydrous ethanol, baking soda and mixed monomers are added and stirred, and after rotary evaporation, a hydrochloric acid solution is added and continued to be stirred, and then filtered, washed and vacuum dried to obtain a carrier, and the carrier is placed in a golden flower fungus suspension, stirred, and allowed to stand to obtain a composite bacterial agent.

2. The method for preparing the golden flower fermented long capsule jute tea according to claim 1, characterized in that: In step 1, the primary fermentation is carried out at a temperature of 25-30° C. for 2-3 days; the volume ratio of the composite bacterial agent to the long capsule jute tea is 3-4:

10.

3. The method for preparing the golden flower fermented long capsule jute tea according to claim 1, characterized in that: In step 2, the secondary fermentation is carried out under continuous illumination at a temperature of 25-30°C and a shaking speed of 120-150 r / min for 2-3 days; the usage ratio of the tea sample and the long capsule jute tea extract is 2.5-3.0 g: 110-120 mL.

4. The method for preparing the golden flower fermented long capsule jute tea according to claim 1, characterized in that: The preparation method of the long capsule jute tea extract comprises the following steps: The long capsule jute tea leaves are washed, dried, ground and sieved to obtain long capsule jute tea powder, and a 60wt% ethanol aqueous solution is added for high temperature extraction, filtered, and after extraction three times, the extracts are combined to obtain a long capsule jute tea extract.

5. The method for preparing the golden flower fermented long capsule jute tea according to claim 4, characterized in that: In the preparation of the long capsule jute tea extract, the sieving is through a 40-mesh sieve; the high temperature extraction is extraction at a temperature of 78-82° C. for 20-30 minutes; the dosage ratio of the long capsule jute tea powder and distilled water is 9-10 g: 100-110 mL; and the moisture content of the long capsule jute tea powder is 6-8%.

6. The method for preparing the golden flower fermented long capsule jute tea according to claim 1, characterized in that: In the preparation of the composite bacterial agent, the ultrasonic treatment is performed at a frequency of 200-300W for 40-60 minutes; and the stirring and mixing is performed at a temperature of 24-28°C for 6-7 hours.

7. The method for preparing the golden flower fermented long capsule jute tea according to claim 1, characterized in that: In the preparation of the composite bacterial agent, the continued stirring is stirring at a speed of 500-800 rpm for 45-60 minutes; the washing is washing three times with deionized water; the vacuum drying is vacuum drying at a temperature of 60°C to constant weight; the stirring treatment is stirring at a speed of 150-200 rpm for 30-40 minutes; and the standing time is 1.5-2.0 hours.

8. The method for preparing the golden flower fermented long capsule jute tea according to claim 1, characterized in that: In the preparation of the composite bacterial agent, the mass ratio of the nanocellulose, cinnamon essential oil, anhydrous ethanol, baking soda, mixed monomer and hydrochloric acid solution is 12-14: 1.8-2.0: 30-40: 0.04-0.06: 2.3-2.8: 40-50; the mass fraction of the hydrochloric acid solution is 3%.

9. The method for preparing the golden flower fermented long capsule jute tea according to claim 1, characterized in that: In the preparation of the composite bacterial agent, the mixed monomer is prepared by mixing phenylalanine and cysteine ​​in a mass ratio of 1.0-1.2:2.5-3.0; the dosage ratio of the carrier and the golden flower fungus suspension is 1.0-1.3 g:30-32 mL; the concentration of the golden flower fungus suspension is 1.2×10 8 -2.0×10 8 CFU / mL.

10. A golden flower fermented long capsule jute tea prepared by the preparation method according to any one of claims 1 to 9.