Monomolecular nano pesticide delivery system and preparation method thereof

Abstract

The invention belongs to the technical field of nano-pesticides, and particularly relates to a single-molecule nano-pesticide delivery system and a preparation method thereof. The structural formula of the sulfate ionic compound provided by the invention is as shown in formula I in the specification. The monomolecular nano-pesticide delivery system provided by the invention is a water-based monomolecular nano-pesticide system and is more beneficial to environmental protection; meanwhile, the particle size of the single-molecule nano pesticide delivery system is about 3 nm, the solubility of the pesticide raw material can be improved, the particle size of the pesticide raw material is reduced, and the insecticidal activity of the pesticide raw material is greatly improved. And the formula I of the # imgabs0 # is shown in the specification.

Description

Technical Field

[0001] The invention belongs to the technical field of nanopesticides, and in particular relates to a single-molecule nanopesticide delivery system and a preparation method thereof. Background Art

[0002] The effect of pesticide application in the field is affected by many factors, and foliar spraying is the first step in field application. Avoiding the loss of pesticide droplets is the key to improving the efficacy of the pesticide. More importantly, the release of the pesticide after spreading on the crop leaves and its diffusion behavior on the target will further affect the effectiveness of the pesticide. The nanopesticide delivery system can significantly improve the effectiveness, safety and target specificity of pesticides by virtue of the unique small size effect, large specific surface area, uniform morphology and structure, and hydrophilic and hydrophobic properties of nanomaterials, thereby overcoming the bottlenecks of traditional pesticide formulations in poor dispersibility, droplet loss and low utilization rate.

[0003] It is worth noting that when pesticides diffuse on the leaf surface, they will face plant cell wall barriers, which are generally less than 20 nm. The stomata of plant leaves are generally at the micron level. In order to enable nanopesticide preparations to better break through these barriers and achieve effective rapid drug release and diffusion, the size of nanopesticides should be further minimized, that is, a single-molecule nanopesticide delivery system. A single-molecule nanopesticide delivery system is a nano delivery system that disperses pesticide molecules into a single-molecule state.

[0004] However, there are currently no reports on water-based single-molecule nanopesticides, which affects the widespread application of single-molecule nanopesticide delivery systems. Summary of the invention

[0005] The purpose of the present invention is to provide a single-molecule nanopesticide delivery system and a preparation method thereof. The sulfate lipid ion compound of the structure shown in Formula I provided by the present invention is used as a carrier to obtain a water-based single-molecule nanopesticide system, which can improve the solubility of the pesticide original drug, reduce the particle size of the pesticide original drug, and greatly improve the insecticidal activity of the pesticide original drug; at the same time, the water-based system is more environmentally friendly.

[0006] In order to achieve the above object, the present invention provides the following technical solutions: The present invention provides an application of a sulfate lipid ion compound as a carrier of a nano pesticide delivery system, wherein the sulfate lipid ion compound has a structural formula as shown in Formula I: Formula I.

[0007] Preferably, the method for preparing the sulfate lipid ion compound of the structure shown in Formula I comprises the following steps: The choline chloride, alkali metal dodecyl sulfate and solvent are mixed and reacted to obtain a sulfate lipid ion compound with a structure shown in I.

[0008] Preferably, the molar ratio of the choline chloride to the alkali metal dodecyl sulfate is 1:0.9-1.5.

[0009] The present invention provides a single-molecule nanopesticide delivery system, comprising a pesticide original, water and a carrier; the carrier is a sulfate lipid ion compound having a structure shown in Formula I; Formula I.

[0010] Preferably, the mass ratio of the pesticide technical to the sulfate lipid ion compound is 1:0.5-5; the mass ratio of the pesticide technical to the water is 1:1-100.

[0011] Preferably, the single-molecule nanopesticide delivery system further comprises a pH regulator; The pH value of the single-molecule nanopesticide delivery system is 4~9.

[0012] Preferably, the pH adjuster includes one or more of acetic acid, citric acid, hydrochloric acid, sulfuric acid, phosphoric acid and nitric acid.

[0013] Preferably, the pesticide technical includes emamectin benzoate (abbreviated as emamectin benzoate) or spinosad.

[0014] The present invention provides a method for preparing the single-molecule nanopesticide delivery system described in the above technical solution, comprising the following steps: The raw materials for preparing the single-molecule nano-pesticide delivery system are mixed to obtain the single-molecule nano-pesticide delivery system.

[0015] Preferably, the mixing temperature is 0-50° C. and the mixing time is 1-72 h.

[0016] The present invention provides an application of a sulfate lipid ion compound as a carrier of a nano pesticide delivery system, wherein the sulfate lipid ion compound has a structural formula as shown in Formula I. The sulfate lipid ion compound of the structure shown in Formula I provided by the present invention is used as a carrier to obtain a water-based single-molecule nano pesticide system, wherein the particle size of the water-based single-molecule nano pesticide system is about 3 nm, thereby improving the solubility of the pesticide original drug, reducing the particle size of the pesticide original drug, and greatly improving the insecticidal activity of the pesticide original drug; and at the same time, using water as a solvent is more environmentally friendly.

[0017] The present invention provides a single-molecule nanopesticide delivery system, including a pesticide original drug, water and a carrier; the carrier is a sulfate lipid ion compound with a structure shown in Formula I. The single-molecule nanopesticide delivery system provided by the present invention is a water-based single-molecule nanopesticide system, which is more environmentally friendly; at the same time, the single-molecule nanopesticide delivery system provided by the present invention has a particle size of about 3 nm, which can improve the solubility of the pesticide original drug, reduce the particle size of the pesticide original drug, and greatly improve the insecticidal activity of the pesticide original drug. The results of the examples show that the emamectin benzoate single-molecule nano preparation prepared in Example 1 has an indoor insecticidal activity LC of Spodoptera exigua 50 The value is significantly lower than that of the commercially available microemulsion and commercially available soluble granules. 50 The value is significantly smaller than that of commercially available aqueous emulsion, commercially available water-dispersible granules and commercially available suspension. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 The dodecyl choline sulfate of the structure shown in formula I prepared in Example 1 1 H NMR (DMSO- d 6) Spectrum; Figure 2 The dodecyl choline sulfate of the structure shown in formula I prepared in Example 1 13 C NMR (DMSO- d 6) Spectrum; Figure 3 The morphology and structural characterization diagram of the emamectin benzoate single-molecule nanoformulation prepared in Example 1; Figure 4 The morphology and structural characterization of the spinosad single-molecule nanoformulation prepared in Example 2; Figure 5 The sulfate lipid ion compound of the structure shown in Formula II prepared in Comparative Example 1 1 H NMR (DMSO- d 6) Spectrum; Figure 6 The sulfate lipid ion compound of the structure shown in Formula II prepared in Comparative Example 1 13 C NMR (DMSO- d 6) Spectrum; Figure 7 This is a physical comparison diagram of the nanopesticide delivery system prepared in Example 1 and Comparative Example 1. DETAILED DESCRIPTION

[0019] The present invention provides an application of a sulfate lipid ion compound as a carrier of a nano pesticide delivery system, wherein the sulfate lipid ion compound has a structural formula as shown in Formula I: Formula I.

[0020] In the present invention, unless otherwise specified, all preparation raw materials / components are commercially available products well known to those skilled in the art.

[0021] In the present invention, the method for preparing the sulfate lipid ion compound of the structure shown in Formula I preferably comprises the following steps: The choline chloride, alkali metal dodecyl sulfate and solvent are mixed and reacted to obtain a sulfate lipid ion compound with a structure shown in I.

[0022] In the present invention, the alkali metal dodecyl sulfate is preferably sodium dodecyl sulfate. The solvent preferably includes one or more of water, ethanol, methanol, acetonitrile, dichloromethane, tetrahydrofuran and acetone, and more preferably includes ethanol aqueous solution or ethanol. The volume content of ethanol in the ethanol aqueous solution is preferably 95%. The molar ratio of the choline chloride to the alkali metal dodecyl sulfate is preferably 1:0.9~1.5, more preferably 1:0.9~1.3, further preferably 1:0.9~1.2, and in the embodiment it can be 1:0.94. The ratio of the mass of the choline chloride to the volume of the solvent is preferably 1g:(1~10)mL, and in the embodiment it can be 1g:7.1mL or 1g:4.4mL. In the present invention, the reaction temperature is preferably 0~50℃, more preferably 15~40℃, and specifically 25℃ in the embodiment; the reaction time is preferably 1~7d, more preferably 2~5d, and specifically 4d in the embodiment. The reaction is carried out under stirring. After the reaction is completed, a reaction liquid is obtained; the present invention preferably separates the reaction liquid into solid and liquid, removes the solvent from the obtained liquid phase product, and obtains the sulfate lipid ion compound. The specific implementation of the solid-liquid separation is preferably filtration. The specific implementation of the solvent removal is preferably reduced pressure distillation.

[0023] The present invention provides a single-molecule nanopesticide delivery system, comprising a pesticide stock solution and a carrier; the carrier is a sulfate lipid ion compound with a structure shown in Formula I; Formula I.

[0024] The single-molecule nanopesticide delivery system provided by the present invention includes a pesticide original drug. In a specific embodiment of the present invention, the pesticide original drug preferably includes emamectin benzoate or spinosad. The emamectin benzoate and spinosad are new biological and efficient insecticides, which have the remarkable characteristics of biological pesticides such as ultra-high efficiency, low toxicity, low residue, and no pollution, and are widely used in the prevention and control of various pests of crops such as vegetables and fruit trees. Emamectin benzoate and spinosad are both slightly soluble in water. The present invention uses the emamectin benzoate and spinosad as model pesticides for single-molecule nanopesticide preparations.

[0025] The single-molecule nanopesticide delivery system provided by the present invention includes water. In the present invention, the water is used as a dispersant of the single-molecule nanopesticide delivery system.

[0026] The single-molecule nanopesticide delivery system provided by the present invention includes a carrier. In the present invention, the carrier is a sulfate lipid ion compound with a structure shown in Formula I.

[0027] The unimolecular nanopesticide delivery system provided by the present invention preferably further comprises a pH regulator. In the present invention, the pH regulator is preferably an acidic pH regulator. The pH regulator preferably comprises one or more of acetic acid, citric acid, hydrochloric acid, sulfuric acid, phosphoric acid and nitric acid.

[0028] In the present invention, the mass ratio of the pesticide technical to the sulfate lipid ion compound is preferably 1:0.5-5, more preferably 1:1-5, further preferably 1:3-5, and specifically 1:5 in the embodiment.

[0029] In the present invention, the mass ratio of the pesticide technical to the water is preferably 1:1-100, more preferably 1:30-100, further preferably 1:50-100, and specifically 1:94 in the embodiment.

[0030] In the present invention, the pH value of the single-molecule nanopesticide delivery system is preferably 4-9, and specifically 5.3±0.2 or 7.0±0.2 in the embodiments.

[0031] The particle size of the single-molecule nanopesticide delivery system provided by the present invention is preferably ≤5 nm (about 3 nm).

[0032] The present invention provides a method for preparing the single-molecule nanopesticide delivery system described in the above technical solution, comprising the following steps: The raw materials for preparing the single-molecule nano-pesticide delivery system are mixed to obtain the single-molecule nano-pesticide delivery system.

[0033] In the present invention, the raw materials for preparing the single-molecule nanopesticide delivery system include pesticide original medicine, water and a carrier; or the raw materials for preparing the single-molecule nanopesticide delivery system include pesticide original medicine, water, a pH regulator and a carrier. In the present invention, the order of mixing the pesticide original medicine, the pH regulator, water and the carrier is preferably: adding the pesticide original medicine and the carrier to water, and then adding the pH regulator.

[0034] In the present invention, the mixing temperature is preferably 0-50°C, more preferably 15-40°C, and specifically 25°C in the embodiment; the mixing time is preferably 1-72h, preferably 12-36h, and specifically 24h in the embodiment.

[0035] In the present invention, the mixed system is directly obtained after the mixing, and the present invention preferably further comprises: performing membrane filtration on the mixed system to obtain the single-molecule nanopesticide delivery system. The pore size of the filter membrane used in the membrane filtration is preferably 0.2 μm, and the number of membrane filtration is preferably 1 to 2 times.

[0036] In order to further illustrate the present invention, the technical solutions provided by the present invention are described in detail below in conjunction with embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0037] Example 1 This embodiment provides a method for preparing dodecyl choline sulfate having a structure shown in Formula I, which is as follows: 140 g of choline chloride and 272 g of sodium dodecyl sulfate were added to 1000 mL of ethanol, and then stirred for reaction at 25°C for 4 days. The obtained mother liquor was filtered, and then the filtered mother liquor was subjected to reduced pressure distillation to remove ethanol to obtain dodecyl sulfate choline ionic liquid. Figure 1 The prepared dodecyl choline sulfate of Example 1 1 H NMR (DMSO- d 6) Spectrum; Figure 2 The prepared dodecyl choline sulfate of Example 1 13 C NMR (DMSO- d 6) Spectrum.

[0038] This embodiment provides a method for preparing a monomolecular nanoformulation of emamectin benzoate, which is as follows: Emamectin benzoate and dodecyl choline sulfate were added to water, acetic acid was added to adjust the pH to 5.3±0.2, and stirred at 25°C for 24 h. After stirring, the mixture was filtered through a 0.2 μm pore size membrane three times to obtain a single-molecule nanopesticide formulation system. The mass ratio of emamectin benzoate, dodecyl choline sulfate and water was 1:5:94. Figure 3 This is a morphology and structural characterization diagram of the emamectin benzoate single-molecule nanoformulation prepared in Example 1. Figure 7 A in FIG. 1 is a physical picture of the single-molecule nanopesticide delivery system in Example 1.

[0039] Example 2 This embodiment provides a method for preparing dodecyl choline sulfate, which is as follows: 140 g of choline chloride and 272 g of sodium dodecyl sulfate were added to 615 mL of 95% ethanol aqueous solution, and then stirred for reaction at 25°C for 4 days. The obtained mother liquor was filtered, and the filtered mother liquor was subjected to reduced pressure distillation to obtain dodecyl sulfate choline ionic liquid.

[0040] This embodiment provides a method for preparing a spinosad single-molecule nanoformulation, which is as follows: Spinosad technical and dodecyl choline sulfate were added to water, acetic acid was added to adjust the pH to 7.0±0.2, and stirred at 25°C for 24 h. After stirring, the mixture was filtered through a 0.2 μm filter membrane twice to obtain a single-molecule nanopesticide formulation system. The mass ratio of spinosad technical, dodecyl choline sulfate and water was 1:5:94. Figure 4 This is the morphology and structural characterization of the spinosad single-molecule nanoformulation prepared in Example 2.

[0041] Test Example 1 (1) The dodecyl choline sulfate prepared in Example 1 was characterized.

[0042] The characterization results are as follows: 1 H NMR (400MHz, D 2 O): δ8.84 (s, 2H), 8.55 (s, 1H), 7.24~7.20 (m, 2H), 4.04~3.40 (m, 7H), 1.27 (t, J=7.4Hz, 3H), as shown in Figure X. 13 C NMR (100MHz, D 2 O): δ147.82, 135.22, 123.24, 121.61, 44.63, 35.53, 14.21, Figure 1 The prepared dodecyl choline sulfate of Example 1 1 H NMR (DMSO- d 6) Spectrum. Figure 2 The prepared dodecyl choline sulfate of Example 1 13 C NMR (DMSO- d 6) Spectrum.

[0043] (2) Morphology and structural characterization of the emamectin benzoate monomolecular nanoformulation prepared in Example 1 Figure 3 The morphology and structural characterization diagram of the emamectin benzoate monomolecular nanoformulation prepared in Example 1, Figure 3 a in the figure is a high resolution transmission electron microscopy (HRTEM) image of the emamectin benzoate monomolecular nanoformulation prepared in Example 1. Figure 3 b in the figure is the hydrated particle size (DLS) of the emamectin benzoate monomolecular nanoformulation prepared in Example 1, Figure 3 c in FIG. 1 is a small angle X-ray scattering (SAXS) spectrum of the emamectin benzoate monomolecular nanoformulation prepared in Example 1. The results show that the nanoformulation size of the emamectin benzoate monomolecular nanoformulation prepared in Example 1 is about 3 nm.

[0044] (3) Indoor insecticidal activity of the emamectin benzoate single-molecule nanoformulation prepared in Example 1 against Spodoptera exigua Experimental method: The biological activity of different emamectin benzoate preparations against the third instar larvae of Spodoptera exigua was determined by indoor immersion method. Six preparations with different concentrations were used, and the emamectin benzoate single-molecule nanoformulation prepared in Example 1 (0.32, 1.6, 8, 40, 62.5 and 100 mg·L -1 ), commercially available microemulsions (0.64, 3.2, 16, 62.5, 80 and 125 mg·L -1 ) and commercially available soluble granules (0.64, 3.2, 16, 62.5, 80 and 125 mg·L -1 ), and water without emamectin benzoate was used as a blank control. The larvae were dried on filter paper and then transferred to plates and fed with fresh, untreated cabbage leaves. Each treatment was replicated four times, with 10 larvae in each replicate. Each culture dish was sealed and placed in a constant temperature incubator at 25°C with 16 h of light and 8 h of darkness. The mortality rate was observed 48 h after treatment. The toxicity regression equation, LC 50 and 95% confidence interval. The results are shown in Table 1. In Table 1, "Nopco 5.7% microemulsion" is produced by Shenzhen Nopoxin Crop Science Co., Ltd. ® Emamectin benzoate 5%, dosage form microemulsion; "Weiyuan Biochemical 5.7% commercially available soluble granules" are emamectin benzoate 5.7%, dosage form soluble granules produced by Hebei Weiyuan Biochemical Co., Ltd.

[0045] Table 1 Indoor insecticidal activity of emamectin benzoate monomolecular nanoformulation prepared in Example 1 against Spodoptera exigua

[0046] From the data in Table 1, it can be seen that the LC of the single-molecule nanoformulation of emamectin benzoate prepared in Example 1 of the present invention against beet armyworm is 50 The value is significantly smaller than that of commercially available microemulsion and commercially available soluble granules.

[0047] Test Example 2 (1) The characterization of the dodecyl choline sulfate prepared in Example 2 is consistent with that in Example 1.

[0048] (2) Morphology and structural characterization of the single-molecule nanoformulation of spinosad prepared in Example 2 Figure 4 This is a morphology and structural characterization diagram of the spinosad single-molecule nanoformulation prepared in Example 2. Figure 4 a in the figure is a high-resolution transmission electron microscopy (HRTEM) image of the spinosad single-molecule nanoformulation prepared in Example 2. Figure 4 b and Figure 4c in the figure is the hydrated particle size (DLS) of the spinosad single-molecule nanoformulation prepared in Example 2, Figure 4 d in FIG. 2 is a small angle X-ray scattering (SAXS) spectrum of the spinosad single molecule nanoformulation prepared in Example 2. The results show that the size of the spinosad single molecule nanoformulation prepared in Example 2 is about 3 nm.

[0049] (3) Indoor insecticidal activity of the spinosad single-molecule nanoformulation prepared in Example 2 against Plutella xylostella Experimental method: The biological activity of the spinosad single-molecule nanoformulation prepared in Example 2 against the second-instar larvae of Plutella xylostella was evaluated by the same method as in Test Example 1. The spinosad single-molecule nanoformulation prepared in Example 2 (0.0156, 0.0625, 0.25, 0.5 and 1 mg·L -1 )、“Nopoxin 8% water emulsion” (0.00625, 0.025, 0.1, 0.4 and 1.6 mg·L -1 ), "Huimin Zhonglian 10% water dispersible granules" (0.02, 0.1, 0.5, 2 and 10 mg·L -1 ) and “Shandong Lukang 10% SC” (0.02, 0.1, 0.5, 2 and 10 mg·L -1 ) to soak the larvae. Calculate the toxicity regression equation, LC 50 and 95% confidence interval. The results are shown in Table 2. In Table 2, "Nopoxin 8% Emulsion in Water" is Asmida produced by Shenzhen Nopoxin Crop Science Co., Ltd. ® 8% water emulsion spinosad. "Huimin Zhonglian 10% water dispersible granules" is Yixi produced by Shandong Huimin Zhonglian Biotechnology Co., Ltd. ® Spinosad 10%, dosage form water dispersible granules. "Shandong Lukang 10% suspension concentrate" is produced by Shandong Lukang Biological Pesticide Co., Ltd. ® Spinosad 10%, dosage form suspension.

[0050] Table 2 Indoor insecticidal activity of the spinosad single-molecule nanoformulation prepared in Example 2 against Plutella xylostella

[0051] From the data in Table 2, it can be seen that the single-molecule nanoformulation of spinosad prepared in Example 2 of the present invention has an insecticidal activity LC against Plutella xylostella. 50 The value is significantly smaller than that of commercially available aqueous emulsion, commercially available water-dispersible granules and commercially available suspension.

[0052] Comparative Example 1 As a control, this comparative example prepared a sulfate lipid ion compound having a structure shown in Formula II.

[0053] Formula II.

[0054] The preparation method of the sulfate lipid ion compound of the structure shown in Formula II is basically the same as that in Example 1, except that the sodium dodecyl sulfate in Example 1 is replaced by sodium dodecyl sulfate, and the specific method includes: adding 140 g of choline chloride and 232 g of sodium dodecyl sulfate to 1000 mL of ethanol, and then stirring the reaction at 25° C. for 4 days, and filtering the obtained mother liquor. The remaining steps are the same as in Example 1.

[0055] The characterization results of the sulfate lipid ion compound of the structure shown in Formula II prepared in Comparative Example 1 are as follows: 1 H NMR (400MHz, D 2 O): δ 5.33 (s, 1H), 3.83 (s, 2H), 3.68 (t, J=8 Hz, 2H), 3.42 (m, 2H), 3.12 (s, 9H), 1.48 (m, 2H), 1.25 (s, 14H), 0.86 (t, J=8 Hz, 3H). 13 CNMR(100MHz,D 2 O): δ 66.91, 65.49, 55.13, 53.05, 31.28, 29.05, 29.02, 28.96, 28.76, 28.69, 25.50, 22.08, 13.93. Figure 5 The sulfate lipid ion compound of the structure shown in Formula II prepared in Comparative Example 1 1 H NMR (DMSO- d 6) Spectrum. Figure 6 The sulfate lipid ion compound of the structure shown in Formula II prepared in Comparative Example 1 13 C NMR (DMSO- d 6) Spectrum.

[0056] This comparative example provides a method for preparing a spinosad preparation, which is as follows: The emamectin benzoate original drug and the sulfate lipid ion compound of the structure shown in formula II were added to water, acetic acid was added to adjust the pH to 5.3 ± 0.2, and stirred at 25°C for 24 h. After the stirring, an attempt was made to pass through a filter membrane with a pore size of 0.2 μm, but it could not be filtered normally, thereby obtaining a formulation system. Among them, the mass ratio of the emamectin benzoate original drug, the sulfate lipid ion compound of the structure shown in formula II and water was 1:5:94. The results of this comparative example show that a single-molecule nano delivery system cannot be obtained, such as Figure 7 As shown in B. Figure 7 A in the figure is the single-molecule nanopesticide delivery system of Example 1.

[0057] From the above examples, it can be seen that the present invention provides a single-molecule nanopesticide delivery system, including pesticide original drug, water and a carrier; the carrier is a sulfate lipid ion compound with a structure shown in Formula I. The present invention provides a single-molecule nanopesticide delivery system, which is a water-based single-molecule nanopesticide system, which is more environmentally friendly; at the same time, the present invention provides a single-molecule nanopesticide delivery system with a particle size of about 3 nm, which can improve the solubility of the pesticide original drug, reduce the particle size of the pesticide original drug, and greatly improve the insecticidal activity of the pesticide original drug. The results of the examples show that the emamectin benzoate single-molecule nano preparation prepared in Example 1 has an indoor insecticidal activity LC of beet armyworm. 50 The value is significantly lower than that of the commercially available microemulsion and commercially available soluble granules. 50 The value is significantly smaller than that of commercially available aqueous emulsion, commercially available water-dispersible granules and commercially available suspension.

[0058] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. Other embodiments can be obtained based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. Application of a sulfate lipid ion compound as a carrier of a nanopesticide delivery system, characterized in that: The structural formula of the sulfate lipid ion compound is shown in Formula I: Formula I.

2. The use according to claim 1, characterized in that: The preparation method of the sulfate lipid ion compound of the structure shown in Formula I comprises the following steps: The choline chloride, alkali metal dodecyl sulfate and solvent are mixed and reacted to obtain a sulfate lipid ion compound with a structure shown in I.

3. The use according to claim 2, characterized in that: The molar ratio of the choline chloride to the alkali metal dodecyl sulfate is 1:0.9-1.

5.

4. A single-molecule nanopesticide delivery system, characterized in that: It comprises pesticide original medicine, water and a carrier; the carrier is a sulfate lipid ion compound with a structure shown in formula I; Formula I.

5. The single-molecule nanopesticide delivery system according to claim 4, characterized in that: The mass ratio of the pesticide technical to the sulfate lipid ion compound is 1:0.5-5; the mass ratio of the pesticide technical to the water is 1:1-100.

6. The single-molecule nanopesticide delivery system according to claim 4, characterized in that: The single-molecule nanopesticide delivery system also includes a pH regulator; The pH value of the single-molecule nanopesticide delivery system is 4-9.

7. The single-molecule nanopesticide delivery system according to claim 6, characterized in that: The pH adjuster includes one or more of acetic acid, citric acid, hydrochloric acid, sulfuric acid, phosphoric acid and nitric acid.

8. The single-molecule nanopesticide delivery system according to any one of claims 4 to 7, characterized in that: The pesticide technical includes avermectin benzoate or spinosad.

9. The method for preparing the single-molecule nanopesticide delivery system according to any one of claims 4 to 8, characterized in that: The following steps are involved: The raw materials for preparing the single-molecule nano-pesticide delivery system are mixed to obtain the single-molecule nano-pesticide delivery system.

10. The preparation method according to claim 9, characterized in that: The mixing temperature is 0-50° C. and the mixing time is 1-72 hours.

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