A single-molecule nanopesticide delivery system and its preparation method

Abstract

This invention belongs to the field of nanopesticide technology, specifically relating to a single-molecule nanopesticide delivery system and its preparation method. The structural formula of the sulfate lipid ion compound provided herein is shown in Formula I. The single-molecule nanopesticide delivery system provided herein is water-based, making it more environmentally friendly. Furthermore, the single-molecule nanopesticide delivery system provided herein has a particle size of approximately 3 nm, which can improve the solubility of the pesticide technical, reduce the particle size of the pesticide technical, and significantly enhance the insecticidal activity of the pesticide technical. #imgabs0# Formula I.

Description

Technical Field

[0001] The present invention belongs to the technical field of nanopesticides, and in particular relates to a single-molecule nanopesticide delivery system and a preparation method thereof. Background Art

[0002] The effectiveness of pesticide application in the field is influenced by numerous factors, and foliar spraying is the first step in field application. Preventing pesticide droplet loss is key to improving efficacy. More importantly, the release of the pesticide after spreading on crop leaves and its diffusion behavior on the target further influence the effectiveness of the pesticide. Nanopesticide delivery systems, leveraging the unique small size effect, large surface area, uniform morphology, and hydrophilic and hydrophobic properties of nanomaterials, can significantly enhance the effectiveness, safety, and target specificity of pesticides, thereby overcoming the bottlenecks of traditional pesticide formulations such as poor dispersibility, droplet loss, and low utilization rates.

[0003] It is worth noting that pesticides face plant cell wall barriers when diffusing across leaves, which are typically smaller than 20 nm. Plant leaf stomata are typically micrometer-sized. To enable nanopesticide formulations to better penetrate these barriers and achieve effective rapid drug release and diffusion, the size of nanopesticides should be minimized, creating a single-molecule nanopesticide delivery system. A single-molecule nanopesticide delivery system is a nanopesticide delivery system that disperses pesticide molecules into a single-molecule state.

[0004] However, there are currently no reports on water-based single-molecule nanopesticides, which affects the widespread application of single-molecule nanopesticide delivery systems. Summary of the Invention

[0005] The purpose of the present invention is to provide a single-molecule nanopesticide delivery system and a preparation method thereof. The sulfate lipid ion compound of the structure shown in Formula I provided by the present invention is used as a carrier to obtain a water-based single-molecule nanopesticide system, which can improve the solubility of the pesticide technical, reduce the particle size of the pesticide technical, and significantly enhance the insecticidal activity of the pesticide technical; at the same time, the water-based system is more environmentally friendly.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] The present invention provides an application of a sulfate lipid ion compound as a carrier of a nanopesticide delivery system. The structural formula of the sulfate lipid ion compound is shown in Formula I:

[0008] Formula I.

[0009] Preferably, the preparation method of the sulfate lipid ion compound of the structure shown in Formula I comprises the following steps:

[0010] The choline chloride, alkali metal lauryl sulfate and solvent are mixed and reacted to obtain a sulfate lipid ion compound with the structure shown in I.

[0011] Preferably, the molar ratio of the choline chloride to the alkali metal lauryl sulfate is 1:0.9-1.5.

[0012] The present invention provides a single-molecule nanopesticide delivery system, comprising a pesticide original, water, and a carrier; the carrier is a sulfate lipid ion compound having a structure shown in Formula I;

[0013] Formula I.

[0014] Preferably, the mass ratio of the pesticide technical to the sulfate lipid ion compound is 1:0.5-5; the mass ratio of the pesticide technical to the water is 1:1-100.

[0015] Preferably, the unimolecular nanopesticide delivery system further comprises a pH regulator;

[0016] The pH value of the single-molecule nanopesticide delivery system is 4~9.

[0017] Preferably, the pH adjuster includes one or more of acetic acid, citric acid, hydrochloric acid, sulfuric acid, phosphoric acid and nitric acid.

[0018] Preferably, the pesticide technical includes emamectin benzoate (abbreviated as emamectin benzoate) or spinosad.

[0019] The present invention provides a method for preparing the single-molecule nanopesticide delivery system described in the above technical solution, comprising the following steps:

[0020] The raw materials for preparing the single-molecule nanopesticide delivery system are mixed to obtain the single-molecule nanopesticide delivery system.

[0021] Preferably, the mixing temperature is 0-50° C. and the mixing time is 1-72 h.

[0022] The present invention provides a use of a sulfate lipid ion compound as a carrier for a nanopesticide delivery system. The structural formula of the sulfate lipid ion compound is shown in Formula I. The sulfate lipid ion compound of Formula I provided by the present invention is used as a carrier to produce a water-based single-molecule nanopesticide system. The water-based single-molecule nanopesticide system has a particle size of approximately 3 nm, thereby increasing the solubility of the pesticide technical, reducing the particle size of the pesticide technical, and significantly enhancing the insecticidal activity of the pesticide technical. Furthermore, the use of water as a solvent is more environmentally friendly.

[0023] The present invention provides a single-molecule nanopesticide delivery system, comprising a pesticide active ingredient, water, and a carrier; the carrier is a sulfate lipid ion compound with a structure represented by Formula I. The single-molecule nanopesticide delivery system provided by the present invention is a water-based single-molecule nanopesticide system, which is more environmentally friendly. Furthermore, the single-molecule nanopesticide delivery system provided by the present invention has a particle size of approximately 3 nm, which can increase the solubility of the pesticide active ingredient, reduce the particle size of the pesticide active ingredient, and significantly enhance the insecticidal activity of the pesticide active ingredient. The results of the examples show that the single-molecule nanoformulation of emamectin benzoate prepared in Example 1 has an indoor insecticidal activity of LC against beet armyworm. 50 The value is significantly lower than that of commercial microemulsion and commercial soluble granules. 50 The value is significantly smaller than that of commercially available aqueous emulsion, commercially available water-dispersible granules and commercially available suspension concentrate. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The structure of the lauryl choline sulfate prepared in Example 1 is shown in Formula I 1 H NMR (DMSO- d 6) Spectrum;

[0025] Figure 2 The structure of the lauryl choline sulfate prepared in Example 1 is shown in Formula I 13 C NMR (DMSO- d 6) Spectrum;

[0026] Figure 3 The morphology and structural characterization diagram of the emamectin benzoate monomolecular nanoformulation prepared in Example 1;

[0027] Figure 4 The morphology and structural characterization of the spinosad single-molecule nanoformulation prepared in Example 2;

[0028] Figure 5 The sulfate lipid ion compound of the structure shown in formula II prepared in Comparative Example 1 1 H NMR (DMSO- d 6) Spectrum;

[0029] Figure 6 The sulfate lipid ion compound of the structure shown in formula II prepared in Comparative Example 1 13 C NMR (DMSO- d 6) Spectrum;

[0030] Figure 7 This is a physical comparison diagram of the nanopesticide delivery system prepared in Example 1 and Comparative Example 1. DETAILED DESCRIPTION

[0031] The present invention provides an application of a sulfate lipid ion compound as a carrier of a nanopesticide delivery system. The structural formula of the sulfate lipid ion compound is shown in Formula I:

[0032] Formula I.

[0033] In the present invention, unless otherwise specified, all preparation raw materials / components are commercially available products well known to those skilled in the art.

[0034] In the present invention, the method for preparing the sulfate lipid ion compound of the structure shown in Formula I preferably comprises the following steps:

[0035] The choline chloride, alkali metal lauryl sulfate and solvent are mixed and reacted to obtain a sulfate lipid ion compound with the structure shown in I.

[0036] In the present invention, the alkali metal dodecyl sulfate is preferably sodium dodecyl sulfate. The solvent preferably includes one or more of water, ethanol, methanol, acetonitrile, dichloromethane, tetrahydrofuran, and acetone, and more preferably includes an aqueous ethanol solution or ethanol. The volume content of ethanol in the aqueous ethanol solution is preferably 95%. The molar ratio of choline chloride to alkali metal dodecyl sulfate is preferably 1:0.9-1.5, more preferably 1:0.9-1.3, even more preferably 1:0.9-1.2, and in embodiments, it can be 1:0.94. The ratio of the mass of choline chloride to the volume of the solvent is preferably 1 g:(1-10) mL, and in embodiments, it can be 1 g:7.1 mL or 1 g:4.4 mL. In the present invention, the reaction temperature is preferably 0-50°C, more preferably 15-40°C, and in embodiments, it is specifically 25°C. The reaction time is preferably 1-7 days, more preferably 2-5 days, and in embodiments, it is specifically 4 days. The reaction is carried out under stirring. After the reaction is completed, a reaction liquid is obtained. In the present invention, the reaction liquid is preferably subjected to solid-liquid separation, and the obtained liquid product is desolventized to obtain the sulfate lipid ion compound. A preferred embodiment of the solid-liquid separation is filtration. A preferred embodiment of the desolventization is vacuum distillation.

[0037] The present invention provides a single-molecule nanopesticide delivery system, comprising a pesticide stock solution and a carrier; the carrier is a sulfate lipid ion compound with a structure shown in Formula I;

[0038] Formula I.

[0039] The single-molecule nanopesticide delivery system provided by the present invention includes a pesticide technical. In specific embodiments of the present invention, the pesticide technical preferably includes emamectin benzoate or spinosad. Emamectin benzoate and spinosad are novel biologically effective insecticides that possess the remarkable characteristics of biopesticides, such as ultra-high efficiency, low toxicity, low residue, and pollution-free properties. They are widely used in the control of various pests in crops such as vegetables and fruit trees. Emamectin benzoate and spinosad are both slightly soluble in water. The present invention uses these two pesticides as model pesticides for single-molecule nanopesticide formulations.

[0040] The single-molecule nanopesticide delivery system provided by the present invention includes water. In the present invention, the water serves as a dispersant of the single-molecule nanopesticide delivery system.

[0041] The single-molecule nanopesticide delivery system provided by the present invention includes a carrier. In the present invention, the carrier is a sulfate lipid ion compound with a structure shown in Formula I.

[0042] The unimolecular nanopesticide delivery system provided by the present invention preferably further comprises a pH regulator. In the present invention, the pH regulator is preferably an acidic pH regulator. The pH regulator preferably comprises one or more of acetic acid, citric acid, hydrochloric acid, sulfuric acid, phosphoric acid, and nitric acid.

[0043] In the present invention, the mass ratio of the pesticide technical to the sulfate lipid ion compound is preferably 1:0.5-5, more preferably 1:1-5, further preferably 1:3-5, and specifically 1:5 in the embodiment.

[0044] In the present invention, the mass ratio of the pesticide technical to the water is preferably 1:1-100, more preferably 1:30-100, further preferably 1:50-100, and specifically 1:94 in the embodiment.

[0045] In the present invention, the pH value of the single-molecule nanopesticide delivery system is preferably 4-9, specifically 5.3±0.2 or 7.0±0.2 in the embodiment.

[0046] The particle size of the single-molecule nanopesticide delivery system provided by the present invention is preferably ≤5 nm (about 3 nm).

[0047] The present invention provides a method for preparing the single-molecule nanopesticide delivery system described in the above technical solution, comprising the following steps:

[0048] The raw materials for preparing the single-molecule nanopesticide delivery system are mixed to obtain the single-molecule nanopesticide delivery system.

[0049] In the present invention, the raw materials for preparing the unimolecular nanopesticide delivery system include the active ingredient pesticide, water, and a carrier; or the raw materials for preparing the unimolecular nanopesticide delivery system include the active ingredient pesticide, water, a pH adjuster, and a carrier. In the present invention, the order of mixing the active ingredient pesticide, pH adjuster, water, and carrier is preferably: adding the active ingredient pesticide and carrier to water, and then adding the pH adjuster.

[0050] In the present invention, the mixing temperature is preferably 0-50°C, more preferably 15-40°C, and specifically 25°C in the embodiment; the mixing time is preferably 1-72h, preferably 12-36h, and specifically 24h in the embodiment.

[0051] In the present invention, the mixed system is directly obtained after the mixing, and the present invention preferably further comprises: membrane filtration of the mixed system to obtain the single-molecule nanopesticide delivery system. The pore size of the filter membrane used in the membrane filtration is preferably 0.2 μm, and the number of membrane filtrations is preferably 1 to 2 times.

[0052] In order to further illustrate the present invention, the technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0053] Example 1

[0054] This embodiment provides a method for preparing lauryl choline sulfate having a structure shown in Formula I, as follows:

[0055] 140 g of choline chloride and 272 g of sodium dodecyl sulfate were added to 1000 mL of ethanol, and then stirred at 25°C for 4 days. The obtained mother liquor was filtered, and then the filtered mother liquor was subjected to reduced pressure distillation to remove ethanol to obtain dodecyl sulfate ionic liquid. Figure 1 The prepared lauryl choline sulfate of Example 1 1 H NMR (DMSO- d 6) Spectrum; Figure 2 The prepared lauryl choline sulfate of Example 1 13 C NMR (DMSO- d 6) Spectrum.

[0056] This embodiment provides a method for preparing a monomolecular nanoformulation of emamectin benzoate, which is as follows:

[0057] Emamectin benzoate technical and dodecyl choline sulfate were added to water, and the pH was adjusted to 5.3±0.2 with acetic acid. The mixture was stirred at 25°C for 24 hours. After stirring, the mixture was filtered through a 0.2 μm pore membrane three times to obtain a single-molecule nanopesticide formulation. The mass ratio of emamectin benzoate technical, dodecyl choline sulfate, and water was 1:5:94. Figure 3 This is a morphology and structural characterization diagram of the emamectin benzoate single-molecule nanoformulation prepared in Example 1. Figure 7 A in FIG. 1 is a physical picture of the single-molecule nanopesticide delivery system in Example 1.

[0058] Example 2

[0059] This embodiment provides a method for preparing lauryl choline sulfate, which is as follows:

[0060] 140 g of choline chloride and 272 g of sodium dodecyl sulfate were added to 615 mL of 95% ethanol aqueous solution, and then stirred at 25°C for 4 days. The obtained mother liquor was filtered and then distilled under reduced pressure to obtain dodecyl sulfate ionic liquid.

[0061] This embodiment provides a method for preparing a spinosad single-molecule nanoformulation, which is as follows:

[0062] Spinosad technical and dodecyl choline sulfate were added to water, and the pH was adjusted to 7.0±0.2 with acetic acid. The mixture was stirred at 25°C for 24 hours. The mixture was then filtered through a 0.2 μm pore membrane twice to obtain a single-molecule nanopesticide formulation. The mass ratio of spinosad technical, dodecyl choline sulfate, and water was 1:5:94. Figure 4 This is the morphology and structural characterization of the spinosad single-molecule nanoformulation prepared in Example 2.

[0063] Test Example 1

[0064] (1) The dodecyl choline sulfate prepared in Example 1 was characterized.

[0065] The characterization results are as follows:

[0066] 1 H NMR (400 MHz, D2O): δ 8.84 (s, 2H), 8.55 (s, 1H), 7.24-7.20 (m, 2H), 4.04-3.40 (m, 7H), 1.27 (t, J = 7.4 Hz, 3H), as shown in Figure X. 13 C NMR (100MHz, D2O): δ147.82, 135.22, 123.24, 121.61, 44.63, 35.53, 14.21, Figure 1 The prepared lauryl choline sulfate of Example 1 1 H NMR (DMSO- d 6) Spectrum. Figure 2 The prepared lauryl choline sulfate of Example 1 13 C NMR (DMSO- d 6) Spectrum.

[0067] (2) Morphology and structural characterization of the emamectin benzoate monomolecular nanoformulation prepared in Example 1

[0068] Figure 3 This is a morphology and structural characterization diagram of the emamectin benzoate single-molecule nanoformulation prepared in Example 1. Figure 3 a in the figure is a high-resolution transmission electron microscopy (HRTEM) image of the emamectin benzoate monomolecular nanoformulation prepared in Example 1. Figure 3 b is the hydrated particle size (DLS) of the emamectin benzoate monomolecular nanoformulation prepared in Example 1, Figure 3 Figure c is a small angle X-ray scattering (SAXS) spectrum of the emamectin benzoate monomolecular nanoformulation prepared in Example 1. The results show that the nanoformulation size of the emamectin benzoate monomolecular nanoformulation prepared in Example 1 is about 3 nm.

[0069] (3) Indoor insecticidal activity of the emamectin benzoate single-molecule nanoformulation prepared in Example 1 against Spodoptera exigua

[0070] Experimental method: The bioactivity of different emamectin benzoate preparations against the third instar larvae of Spodoptera exigua was determined by the indoor immersion method. Six preparations with different concentrations were used, including the emamectin benzoate single-molecule nanoformulation prepared in Example 1 (0.32, 1.6, 8, 40, 62.5, and 100 mg·L -1 ), commercially available microemulsions (0.64, 3.2, 16, 62.5, 80, and 125 mg·L -1 ) and commercially available soluble granules (0.64, 3.2, 16, 62.5, 80, and 125 mg·L -1 ), and water without emamectin benzoate served as a blank control. Larvae were dried on filter paper and then transferred to plates and fed fresh, untreated cabbage leaves. Each treatment was replicated four times, with 10 larvae per replicate. Each plate was sealed and placed in a 25°C incubator with a 16-h light:8-h dark cycle. Mortality was observed 48 hours after treatment. Toxicity regression equations, LC values, and the toxicity regression equations were calculated. 50 and 95% confidence interval. The results are shown in Table 1. In Table 1, "Nopco 5.7% microemulsion" is a product of Shenzhen Nopoxin Crop Science Co., Ltd. ®Emamectin benzoate 5%, dosage form microemulsion; "Weiyuan Biochemical 5.7% commercially available soluble granules" are emamectin benzoate 5.7%, dosage form soluble granules produced by Hebei Weiyuan Biochemical Co., Ltd.

[0071] Table 1 Indoor insecticidal activity of emamectin benzoate monomolecular nanoformulation prepared in Example 1 against Spodoptera exigua

[0072]

[0073] From the data in Table 1, it can be seen that the LC value of the single-molecule nanoformulation of emamectin benzoate prepared in Example 1 of the present invention against Spodoptera exigua is 50 The value is significantly smaller than that of commercial microemulsion and commercial soluble granules.

[0074] Test Example 2

[0075] (1) The characterization of the lauryl choline sulfate prepared in Example 2 is consistent with that in Example 1.

[0076] (2) Morphology and structural characterization of the spinosad single-molecule nanoformulation prepared in Example 2

[0077] Figure 4 This is a morphology and structural characterization diagram of the spinosad single-molecule nanoformulation prepared in Example 2. Figure 4 a in the figure is a high-resolution transmission electron microscopy (HRTEM) image of the spinosad single-molecule nanoformulation prepared in Example 2. Figure 4 b and Figure 4 c in the figure is the hydrated particle size (DLS) of the spinosad single-molecule nanoformulation prepared in Example 2. Figure 4 d in FIG. 5 is a small-angle X-ray scattering (SAXS) spectrum of the spinosad single-molecule nanoformulation prepared in Example 2. The results show that the size of the spinosad single-molecule nanoformulation prepared in Example 2 is about 3 nm.

[0078] (3) Indoor insecticidal activity of the spinosad single-molecule nanoformulation prepared in Example 2 against Plutella xylostella

[0079] Experimental method: The same method as in Test Example 1 was used to evaluate the biological activity of the spinosad single-molecule nanoformulation prepared in Example 2 against the second-instar larvae of Plutella xylostella. The spinosad single-molecule nanoformulation prepared in Example 2 (0.0156, 0.0625, 0.25, 0.5 and 1 mg·L -1 ), “Nopco 8% Emulsion in Water” (0.00625, 0.025, 0.1, 0.4 and 1.6 mg·L -1 ), “Huimin Zhonglian 10% water dispersible granules” (0.02, 0.1, 0.5, 2 and 10 mg·L -1) and “Shandong Lukang 10% SC” (0.02, 0.1, 0.5, 2 and 10 mg·L -1 ) Soak the larvae. Calculate the toxicity regression equation, LC 50 and 95% confidence interval. The results are shown in Table 2. In Table 2, "Nopco 8% Emulsion in Water" is Asmida produced by Shenzhen Nopoxin Crop Science Co., Ltd. ® 8% water emulsion spinosad. "Huimin Zhonglian 10% water dispersible granules" is Yixi produced by Shandong Huimin Zhonglian Biotechnology Co., Ltd. ® Spinosad 10%, dosage form water dispersible granules. "Shandong Lukang 10% suspension concentrate" is produced by Shandong Lukang Biopesticide Co., Ltd. ® Spinosad 10%, dosage form: suspension concentrate.

[0080] Table 2 Indoor insecticidal activity of the spinosad single-molecule nanoformulation prepared in Example 2 against Plutella xylostella

[0081]

[0082] From the data in Table 2, it can be seen that the spinosad single-molecule nanoformulation prepared in Example 2 of the present invention has an insecticidal activity LC against Plutella xylostella. 50 The value is significantly smaller than that of commercially available aqueous emulsion, commercially available water-dispersible granules and commercially available suspension concentrate.

[0083] Comparative Example 1

[0084] As a control, this comparative example prepared a sulfate lipid ion compound having a structure shown in Formula II.

[0085] Formula II.

[0086] The preparation method for the sulfate lipid ion compound of Formula II is substantially the same as that in Example 1, except that sodium lauryl sulfate is substituted for sodium dodecyl sulfate. The method comprises adding 140 g of choline chloride and 232 g of sodium dodecyl sulfate to 1000 mL of ethanol, stirring the mixture at 25°C for 4 days, and filtering the resulting mother liquor. The remaining steps are the same as in Example 1.

[0087] The characterization results of the sulfate lipid ion compound of the structure shown in Formula II prepared in Comparative Example 1 are as follows:

[0088] 1H NMR (400MHz, D2O): δ 5.33 (s, 1H), 3.83 (s, 2H), 3.68 (t, J=8 Hz, 2H), 3.42 (m, 2H), 3.12 (s, 9H), 1.48 (m, 2H), 1.25 (s, 14H), 0.86 (t, J=8 Hz, 3H). 13 CNMR (100MHz, D2O): δ 66.91, 65.49, 55.13, 53.05, 31.28, 29.05, 29.02, 28.96, 28.76, 28.69, 25.50, 22.08, 13.93. Figure 5 The sulfate lipid ion compound of the structure shown in formula II prepared in Comparative Example 1 1 H NMR (DMSO- d 6) Spectrum. Figure 6 The sulfate lipid ion compound of the structure shown in formula II prepared in Comparative Example 1 13 C NMR (DMSO- d 6) Spectrum.

[0089] This comparative example provides a method for preparing a spinosad preparation, which is as follows:

[0090] The emamectin salt original drug and the sulfate lipid ion compound of the structure shown in formula II were added to water, acetic acid was added to adjust the pH to 5.3 ± 0.2, and stirred at 25 ° C for 24 h. After the stirring was completed, an attempt was made to pass through a filter membrane with a pore size of 0.2 μm, but it could not be filtered normally to obtain a formulation system. Among them, the mass ratio of the emamectin salt original drug, the sulfate lipid ion compound of the structure shown in formula II and water was 1:5:94. The results of this comparative example show that a single-molecule nano-delivery system cannot be obtained, such as Figure 7 As shown in B. Figure 7 A in FIG is the single-molecule nanopesticide delivery system of Example 1.

[0091] From the above examples, it can be seen that the present invention provides a single-molecule nanopesticide delivery system, including a pesticide, water, and a carrier; the carrier is a sulfate lipid ion compound with a structure shown in Formula I. The present invention provides a single-molecule nanopesticide delivery system, which is a water-based single-molecule nanopesticide system, which is more environmentally friendly; at the same time, the present invention provides a single-molecule nanopesticide delivery system with a particle size of about 3 nm, which can improve the solubility of the pesticide, reduce the particle size of the pesticide, and significantly improve the insecticidal activity of the pesticide. The results of the examples show that the emamectin benzoate single-molecule nanoformulation prepared in Example 1 has an indoor insecticidal activity LC against beet armyworm. 50The value is significantly lower than that of commercial microemulsion and commercial soluble granules. 50 The value is significantly smaller than that of commercially available aqueous emulsion, commercially available water-dispersible granules and commercially available suspension concentrate.

[0092] Although the above embodiment provides a detailed description of the present invention, it is only a part of the embodiments of the present invention, not all of the embodiments. Other embodiments can be obtained based on this embodiment without creativity, and these embodiments all fall within the scope of protection of the present invention.

Claims

1. A single-molecule nanopesticide delivery system, characterized in that: The invention comprises a pesticide technical, water, a carrier, and a pH regulator; the carrier is a sulfate lipid ion compound having a structure represented by Formula I; the particle size of the single-molecule nanopesticide delivery system is 3 nm, the pesticide technical is emamectin benzoate; the mass ratio of the pesticide technical to the sulfate lipid ion compound is 1:5; the mass ratio of the pesticide technical to the water is 1:94; the pH regulator is acetic acid; and the pH value of the single-molecule nanopesticide delivery system is 5.3±0.2; Formula I; The preparation method of the single-molecule nanopesticide delivery system includes: adding emamectin benzoate and the carrier into water, adding acetic acid to adjust the pH value to 5.3±0.2, stirring at 25°C for 24 hours, and passing through a filter membrane with a pore size of 0.2 μm three times after stirring to obtain the single-molecule nanopesticide delivery system.

2. A single-molecule nanopesticide delivery system, characterized in that: The invention comprises a pesticide active ingredient, water, a carrier, and a pH regulator; the carrier is a sulfate lipid ion compound having a structure represented by Formula I; the particle size of the single-molecule nanopesticide delivery system is 3 nm, the pesticide active ingredient is spinosad; the mass ratio of the pesticide active ingredient to the sulfate lipid ion compound is 1:5; the mass ratio of the pesticide active ingredient to the water is 1:94; the pH regulator is acetic acid; and the pH value of the single-molecule nanopesticide delivery system is 7.0±0.2; Formula I; The preparation method of the single-molecule nanopesticide delivery system includes: adding spinosad and the carrier into water, adding acetic acid to adjust the pH value to 7.0±0.2, stirring at 25°C for 24 hours, and passing through a filter membrane with a pore size of 0.2 μm twice after stirring to obtain the single-molecule nanopesticide delivery system.

3. The unimolecular nanopesticide delivery system according to claim 1 or 2, characterized in that: The preparation method of the sulfate lipid ion compound of the structure shown in Formula I comprises the following steps: Choline chloride, alkali metal lauryl sulfate and a solvent are mixed and reacted to obtain a sulfate lipid ion compound with a structure shown in formula I.

4. The single-molecule nanopesticide delivery system according to claim 3, characterized in that: The molar ratio of the choline chloride to the alkali metal lauryl sulfate is 1:0.9-1.5.

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