Spodoptera frugiperda attractant and application thereof
By developing a fall armyworm attractant containing specific odor compounds, combined with traps or chemical pesticides, the problem of unreasonable use of traditional chemical pesticides has been solved, and effective collection and capture of fall armyworms has been achieved, which has significantly improved the prevention and control effect of corn production.
Patent Information
- Application Number
- CN202510176418.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-18
- Publication Date
- 2025-05-23
AI Technical Summary
The existing technology is difficult to effectively prevent and control the threat of fall armyworm to corn production by fall armyworm. The unreasonable use of traditional chemical pesticides has led to intensification of environmental pollution and pest resistance, and differences in insect species have led to differences in olfactory perception, which limits the research and development and application of efficient attractants.
A fallopian moth attractant is provided, containing odorous compounds such as β-caryophyllene, α-pinene, cis-3-hexene-1 alcohol and 6-methyl-5-hexene-2one. The inducement and capture of fallopian moth is achieved by hanging the inducement and combining a trap or a chemical insecticide.
The egg laying volume of corn plants by female adults of Fattish grassland has been significantly improved, and the purpose of harm control is achieved through reducing and increasing efficiency, and has wide application prospects.
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Figure CN120021619A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of attractants, and in particular relates to a fall armyworm attractant and application thereof. Background Art
[0002] Fall armyworm Spodopterafrugiperda (JESmith), belonging to the family Noctuidae of the order Lepidoptera, is a major migratory agricultural pest. It poses a huge threat to corn production. At present, chemical control is the main means of prevention and control, which is difficult to fundamentally solve the problem. The long-term irrational use of traditional chemical pesticides will cause problems such as corn quality decline, ecological environment pollution, and increased pest resistance. Therefore, it is urgent to develop and reserve new prevention and control technologies.
[0003] Based on the principles of insect chemical ecology, new pest control technologies represented by attractants have gained increasing attention and achieved good control effects. Studying the behavioral responses of pests to volatile small molecule compounds is an important area for the development and utilization of attractants, but differences in insect species can lead to differences in olfactory perception. The recognition mechanism of chemical perception of fall armyworm is still unclear, which limits the development and application of efficient attractants. Summary of the invention
[0004] In view of this, the object of the present invention is to provide a fall armyworm attractant, which has a good attracting effect on the fall armyworm.
[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:
[0006] A fall armyworm attractant comprises an odor compound, wherein the odor compound is any one of β-caryophyllene, α-pinene, cis-3-hexene-1-ol and 6-methyl-5-heptene-2-one.
[0007] Preferably, when the odor compound is β-caryophyllene or α-pinene, the concentration of the odor compound in the fall armyworm attractant is 5 to 15 μL / mL.
[0008] Preferably, when the odor compound is cis-3-hexen-1-ol or 6-methyl-5-hepten-2-one, the concentration of the odor compound in the fall armyworm attractant is 0.5 to 3 μL / mL.
[0009] Preferably, the fall armyworm attractant also includes an organic solvent.
[0010] Preferably, the organic solvent is n-hexane.
[0011] Another object of the present invention is to provide the use of the fall armyworm attractant in attracting the fall armyworm.
[0012] Preferably, the fall armyworm attractant is used to attract fall armyworm larvae and / or adults.
[0013] Preferably, the odor compounds in the fall armyworm attractant have a strong ability to bind to odor binding proteins, and the odor binding proteins include SfruOBP20 and SfruOBP29.
[0014] Preferably, the odorant binding protein SfruOBP20 and the odorant binding protein SfruOBP29 are highly expressed in the antennae of Spodoptera frugiperda adults.
[0015] Another object of the present invention is to provide the use of the fall armyworm attractant in attracting female adults of the fall armyworm to lay eggs.
[0016] Compared with the prior art, the present invention has the following beneficial effects:
[0017] The present invention provides a fall armyworm attractant, which has an attracting effect on the behavioral response of fall armyworm host searching and locating eggs, and fall armyworm can be lured by hanging the attractant. In a field test, there was a significant difference in the egg laying amount of female fall armyworm on corn plants without hanging attractants and corn plants hanging 10μL / mL β-caryophyllene, and the average number of eggs was (229.00±155.99b) and (851.33±383.39a), respectively. The fall armyworm attractant provided by the present invention can also be used in conjunction with a trap or in combination with a variety of chemical pesticides to capture and kill fall armyworm, achieving the purpose of pest control while reducing the amount and increasing the efficiency, and has a wide range of application prospects. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 The three-dimensional structural model of the fall armyworm odor-binding protein;
[0019] Figure 2 Verify-3D scoring results for the fall armyworm odorant binding protein SfruOBP20 model;
[0020] Figure 3 Verify-3D scoring results for the fall armyworm odorant binding protein SfruOBP29 model;
[0021] Figure 4 The residue error value of the modeled structure of the fall armyworm odor-binding protein calculated by ERRAT;
[0022] Figure 5 is the binding status of SfruOBP20 and beta-Caryophyllene;
[0023] Figure 6 is the binding status of SfruOBP29 and beta-Caryophyllene;
[0024] Figure 7 It is a schematic diagram of the connection of the Y-type olfactometer, wherein the upper part is a top view of the olfactory reaction chamber of the Y-type olfactometer, and the lower part is a schematic diagram of the connection of the Y-type olfactometer;
[0025] Figure 8 The directional behavioral response of unmated adults of Spodoptera frugiperda to 12 volatiles at a concentration of 10 μL / mL. NC was selected as the directional behavioral response of the control group.
[0026] Fig. 9 The directional behavioral response of unmated adults of Spodoptera frugiperda to 12 volatiles at a concentration of 1 μL / mL. NC was selected as the directional behavioral response of the control group.
[0027] Fig.10 Schematic diagram of the indoor double-choice test;
[0028] Fig.11 Schematic diagram of the double-choice experiment in the field. DETAILED DESCRIPTION
[0029] The present invention provides a fall armyworm attractant, wherein the odor compound in the fall armyworm attractant is any one of β-caryophyllene, α-pinene, cis-3-hexene-1-ol and 6-methyl-5-heptene-2-one. Differences in insect species can lead to differences in olfactory perception, and the same volatile has different effects on different types of insects. The odor compound in the fall armyworm attractant of the present invention has a significant attracting effect on the fall armyworm.
[0030] In the present invention, when the odor compound is β-caryophyllene or α-pinene, the concentration of the odor compound in the fall armyworm attractant is 5 to 15 μL / mL, preferably 8 to 12 μL / mL, and more preferably 10 μL / mL; when the odor compound is cis-3-hexene-1-ol or 6-methyl-5-heptene-2-one, the concentration of the odor compound in the fall armyworm attractant is 0.5 to 3 μL / mL, preferably 0.7 to 1.5 μL / mL, and more preferably 1 μL / mL. When different volatile substances attract fall armyworms, the concentrations that act are different. The concentration of the odor compound in the fall armyworm attractant provided by the present invention has a good attracting effect on fall armyworms.
[0031] In the present invention, the fall armyworm attractant also includes an organic solvent, and preferably, the organic solvent is n-hexane.
[0032] The fall armyworm attractant of the present invention can be used to attract the fall armyworm, and can be used in conjunction with a trap or a variety of chemical pesticides to capture and kill the fall armyworm, thereby achieving the purpose of pest control while reducing the amount and increasing the efficiency.
[0033] The present invention also provides the use of the fall armyworm attractant in attracting fall armyworm, wherein the fall armyworm attractant is used to attract fall armyworm larvae and / or adults, and the fall armyworm attractant used to attract fall armyworm adults is preferably used to attract magnetic adults.
[0034] In the present invention, the odor compounds in the fall armyworm attractant have a strong ability to bind to odor binding proteins, and the odor binding proteins include SfruOBP20 and SfruOBP29; the odor binding protein SfruOBP20 and the odor binding protein SfruOBP29 are highly expressed in the antennae of fall armyworm adults. The insect chemosensory pathway mainly receives pheromone compounds through the combination of allelopathic proteins and small molecule compounds. Antennae are important olfactory organs for sensing and identifying plant odor compounds. The odor binding proteins SfruOBP20 and the odor binding protein SfruOBP29, which are highly expressed in the antennae of fall armyworm adults, have a strong ability to bind to the odor compounds in the attractant provided by the present invention, which affects the attractant's host selection and oviposition positioning process for fall armyworms.
[0035] The present invention also provides the use of the fall armyworm attractant in inducing female adults of the fall armyworm to lay eggs. The fall armyworm attractant in the present invention affects the egg-laying positioning process of the fall armyworm and significantly induces female adults of the fall armyworm to lay eggs.
[0036] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.
[0037] Example 1
[0038] A fall armyworm attractant: the odor compound is β-caryophyllene, and the β-caryophyllene is mixed with n-hexane to obtain the fall armyworm attractant with a β-caryophyllene concentration of 10 μL / mL.
[0039] Example 2
[0040] The difference between this embodiment and embodiment 1 is that the concentration of β-caryophyllene is 5 μL / mL.
[0041] Example 3
[0042] The difference between this embodiment and embodiment 1 is that the concentration of β-caryophyllene is 12 μL / mL.
[0043] Example 4
[0044] The difference between this embodiment and embodiment 1 is that the concentration of β-caryophyllene is 15 μL / mL.
[0045] Example 5
[0046] A fall armyworm attractant: the odor compound is alpha-pinene, and the alpha-pinene is mixed with n-hexane to obtain the fall armyworm attractant with an alpha-pinene concentration of 10 μL / mL.
[0047] Example 6
[0048] The difference between this embodiment and embodiment 5 is that the concentration of α-pinene is 5 μL / mL.
[0049] Example 7
[0050] The difference between this embodiment and embodiment 5 is that the concentration of α-pinene is 12 μL / mL.
[0051] Example 8
[0052] The difference between this embodiment and embodiment 5 is that the concentration of α-pinene is 15 μL / mL.
[0053] Example 9
[0054] A fall armyworm attractant: the odor compound is cis-3-hexen-1-ol, cis-3-hexen-1-ol is mixed with n-hexane to obtain a fall armyworm attractant with a cis-3-hexen-1-ol concentration of 1 μL / mL.
[0055] Example 10
[0056] The difference between this embodiment and embodiment 9 is that the concentration of cis-3-hexen-1-ol is 0.5 μL / mL.
[0057] Embodiment 11
[0058] The difference between this embodiment and embodiment 9 is that the concentration of cis-3-hexen-1-ol is 1.5 μL / mL.
[0059] Example 12
[0060] The difference between this embodiment and embodiment 9 is that the concentration of cis-3-hexen-1-ol is 2 μL / mL.
[0061] Example 13
[0062] A fall armyworm attractant: the odor compound is 6-methyl-5-heptene-2-one, 6-methyl-5-heptene-2-one is mixed with n-hexane to obtain a fall armyworm attractant with a 6-methyl-5-heptene-2-one concentration of 1 μL / mL.
[0063] Embodiment 14
[0064] The difference between this embodiment and embodiment 13 is that the concentration of 6-methyl-5-heptene-2-one is 0.5 μL / mL.
[0065] Embodiment 15
[0066] The difference between this embodiment and embodiment 13 is that the concentration of 6-methyl-5-heptene-2-one is 1.5 μL / mL.
[0067] Example 16
[0068] The difference between this embodiment and embodiment 13 is that the concentration of 6-methyl-5-heptene-2-one is 2 μL / mL.
[0069] Test Example 1
[0070] Binding of odorant compounds by the Spodoptera frugiperda odorant-binding protein.
[0071] 1. The information of the compounds used for testing is shown in Table 1. The PDB files of the ligand molecules were obtained from the organic small molecule biological activity database PUBCHEM (https: / / pubchem.ncbi.nlm.nih.gov / ).
[0072] Table 1 Odor compound information
[0073]
[0074]
[0075] 2. Evaluation of the three-dimensional structure of odorant binding proteins
[0076] Swiss-model (https: / / www.swissmodel.expasy.org / ) was used for homology modeling to predict the protein structure of SfruOBP20 and SfruOBP29 protein structures. Procheck, Verify-3D and ERRAT were then used to analyze the constructed models to determine the rationality of the constructed models.
[0077] 3. Autodock Molecular Docking
[0078] The odor binding protein model and ligand compound were energy optimized using Autodock Tools, atomic charges were assigned, non-polar hydrogen atoms were fused, etc., and the optimized receptors and ligands were formatted. Autodock software was used for docking, the highest-rated ligand conformation was selected for integration with the receptor protein, and PyMOL software was used for image generation and analysis.
[0079] 4. Results
[0080] 4.1 Modeling Results
[0081] The modeling results show (Table 2, Figure 1 ), SfruOBP20 and SfruOBP29 protein structures both have 6-helices and 6 conserved cysteines. The SfruOBP20 helical structure is composed of -1 (Val21-Ser42), -2 (Phe52-Trp56), -3 (Arg65-Phe78), -4 (His89-Ser98), -5 (Gly102-His120) and -6 (His126-Arg144), and three disulfide bonds are formed by Cys38 and Cys73, Cys69 and Cys127, Cys116 and Cys136, connecting -1 and -3, -3 and -6, -5 and -6, respectively. SfruOBP29 helix structure -1 (Tyr21-Lys36), -2 (Asp40-Thr47), -3 (Arg55-Tyr68), -4 (Pro78-Leu89), -5 (Pro93-Ser112) and -6 (Glu117-Met135); Cys32 and Cys63, Cys59 and Cys118, Cys106 and Cys127 form three pairs of disulfide bonds, connecting -1 and -3, -3 and -6, -5 and -6, respectively.
[0082] Table 2 Modeling information of fall armyworm odor binding protein
[0083] name stencil Template Description Sequence consistency Coverage OBP Classification SfruOBP20 2wc6.1.A BmorGOBP2 50.71％ 0.95 GeneralOBP SfruOBP29 6qq4.2.A DmelOBP28a 36.50％ 0.85 OBP
[0084] 4.2 Model Evaluation
[0085] (1) Verify-3D evaluation
[0086] The results are as follows Figure 2 , Figure 3 As shown in the figure, the rationality of modeling is determined by comparing the compatibility of the amino acid sequence with the helical and folded structures in the atomic model. When the compatibility between the three-dimensional structure and the primary structure of an amino acid sequence is greater than 0.2, the constructed model structure is judged to be reasonable. For SfruOBP20 and SfruOBP29, 91.43% and 89.08% of the scores between the three-dimensional structure and the primary structure of the amino acids are greater than 0.2, respectively.
[0087] (2) Procheck evaluation
[0088] The stereochemical quality of the protein structure is evaluated by analyzing the geometry of the amino acid residues and the overall structure geometry. When more than 90% of the non-glycine and non-proline residues are located in the reasonable region, the protein model is considered reasonable. The experimental results show that the non-glycine and non-proline residues of SfruOBP20 and SfruOBP29 are all located in the reasonable region.
[0089] (3) ERRAT evaluation
[0090] like Figure 4 As shown, ERRAT counts the non-bonded interactions between different types of atoms. When the ERRAT value is greater than 50%, the constructed protein model has a higher credibility. In the present invention, the ERRAT values of SfruOBP20 and SfruOBP29 are 86.275% and 94.595%, respectively, which are significantly higher than the basic values, and the rationality of the two constructed protein models is relatively high.
[0091] 4.3 Molecular docking
[0092] Binding energy can reflect the binding situation between receptor protein and ligand molecule. The smaller the binding energy, the easier it is for the protein to bind to the ligand and the stronger the binding ability. The binding mode of the two odor binding proteins with the main components of the host volatiles was analyzed by Autodock. The docking results of the two odor binding proteins with 17 ligand small molecules showed (Table 3) that SfruOBP20 and SfruOBP29 had good affinity with the ligand compounds. Among all the ligands, SfruOBP20 and SfruOBP29 bound best to β-caryophyllene, followed by cyclophane and trans-nerolidol, while the binding ability to substances such as 1-hexanol, 1-heptanol and 2-hexanone was weaker. The docking conformations of SfruOBP20 and SfruOBP29 with ligand small molecules are shown in Figure 3. Figure 5 and Figure 6 It was shown that β-caryophyllene was located in the cavity formed by the α-helix and did not form hydrogen bonds with the receptor protein, but interacted through hydrophobic forces.
[0093] Table 3 Binding energy of Spodoptera frugiperda odorant binding protein docking with odorant compounds
[0094]
[0095]
[0096] It can be seen that the binding energy between S. frugiperda SfruOBP20 and odor compounds is concentrated between -8.29 and -3.78 KJ / mol. Among them, SfruOBP20 has the best binding ability with the terpene compound β-caryophyllene and the smallest binding energy. The binding energy between S. frugiperda SfruOBP29 and odor compounds is concentrated between -7.75 and -3.25 KJ / mol. Among them, SfruOBP29 has the best binding ability with the terpene compound β-caryophyllene and the smallest binding energy.
[0097] Test Example 2
[0098] Behavioral responses of Fall Armyworm to odor compounds.
[0099] 1. Test insect source
[0100] Fall armyworms were raised in the insect breeding room of the Anhui University of Science and Technology plantation. Newly hatched larvae were raised in transparent rectangular feed boxes, fed with fresh corn leaves daily and the filter paper in the box was replaced in time to keep the plastic box clean. When the larvae grew to the fifth instar, they were placed in a round feed box and fed with feed blocks. After the larvae pupated, the male and female larvae were distinguished and placed in transparent boxes separately to wait for them to emerge as adults. The breeding environment conditions were as follows: temperature 26℃±1℃, relative humidity 60%~80%, light time: dark time = 16h:8h, and adults were fed with 10% honey water. Unmated female and male moths with complete morphology after emergence and normal flight were selected for the experiment.
[0101] 2. Instruments and Equipment
[0102] Y-type olfactometer, drying tower (500mL; 3 pieces), activated carbon (1kg / bag), vacuum pump, medical silicone tube (3 pieces), electronic balance, gas collecting bottle, glass rod, pear-shaped bottle, gas washing bottle, beaker, and pipette.
[0103] 3. Test odor compounds
[0104] The names, CAS numbers and purchasing companies of the tested chemical odorous compounds are shown in Table 4. The tested chemical odorous compounds were prepared with n-hexane into two concentration gradients of 1 μL / mL and 10 μL / mL, with n-hexane as the control.
[0105] Table 4 Relevant information of the test compounds
[0106] Compound English name CAS Number company Cis-3-Hexenyl acetate z-3-hexenyl-phenylacetate 3681-71-8 J&K Linalool Linalool 78-70-6 J&K trans-Nerolidol Nerolidol 40716-66-3 ALORICH (-)-β-pinene (-)-beta-Pinene 18172-67-3 ALORICH 2-Pentylfuran 2-Pentylfuran 3777-69-3 J&K (+)-α-Pinene (+)-alpha-Pinene 80-56-8 J&K 6-Methyl-5-hepten-2-one 6-Methyl-5-hepten-2-one 110-93-0 J&K 2-Hexanone 2-Hexanone 591-78-6 ALORICH cis-3-hexen-1-ol Cis-3-Hexen-1-ol 928-96-1 J&K β-Caryophyllene beta-Caryophyllene 87-44-5 J&K 1-Hexanol 1-Hexanol 111-27-3 Seklin 1-Heptanol 1-Heptanol 111-70-6 Seklin n-Hexane n-Hexane 110-54-3 J&K
[0107] 4. Y-type olfactometer behavioral assay
[0108] The Y-type olfactometer consists of a drying tower, an airflow meter, a gas washing bottle, and a three-arm Y-shaped tube connected by a silicone tube. Plug one end of the three-arm olfactometer with absorbent cotton, and connect the other two ends to two 250mL odor source bottles ( Figure 7), 30mL of the test compound with different concentration gradients was added to the odor source bottle of the treatment group, and 30mL of n-hexane solution was added to the control group. Experimental conditions: temperature (24±1)℃, relative humidity (40±5)%. The flow rate was adjusted to 0.5L / min by the airflow meter. The male and female adults of the fall armyworm within 1 day of emergence were placed in the olfactometer respectively and observed for 5 minutes. The volatiles that first selected more than 1 / 2 of one arm and lasted for more than 30 seconds were taken as the tropism odor source. Each group treated 30 heads, and the arms were cleaned and changed every 5 heads. SPSS21.0 was used to perform chi-square test analysis and difference significance analysis on the experimental data. X 2 It represents the value of chi-square test, P represents significant difference, P < 0.05 is recorded as significant difference, P < 0.01 is extremely significant difference. The test insects that did not make a choice in the experiment were not included in the data processing. The choice response rate of adults in the experimental group and the control group was calculated by the ratio of the number of responding insects to the total number of insects.
[0109] The directional behavior of unmated adults of Spodoptera frugiperda to 12 compounds was measured by a Y-type olfactometer. The results showed that compared with the n-hexane control, 10 μL / mL of β-caryophyllene and α-pinene had a very significant attractant effect on unmated female moths, with the selection response rates being 77.27% (X 2 =10.880; P = 0.001) and 75.00% (X 2 =6.667; P = 0.010), 10 μL / mL of β-pinene had a significant attracting effect on unmated male moths, with a selection response rate of 76.92% (X 2 =4.969; P=0.026)( Figure 8 1 μL / mL of cis-3-hexen-1-ol and 6-methyl-5-hepten-2-one had a significant attractant effect on unmated female moths, with a selection response rate of 83.33% (X 2 =4.400; P=0.036) and 64.29% (X 2 =5.417; P = 0.020), 1 μL / mL of β-pinene and 2-hexanone had a significant attractant effect on unmated male moths, with the selection response rates being 83.33% (X 2 =4.400; P = 0.036) and 71.43% (X 2 =4.194; P = 0.041)( Fig. 9 ). However, the six compounds, cis-3-hexenyl acetate, 2-pentylfuran, 1-heptanol, trans-nerolidol, 2-hexanone and 1-hexanol, showed no significant activity against unmated male and female adults of Spodoptera frugiperda.
[0110] 5. Indoor baiting test
[0111] The compound β-caryophyllene, which has attractant activity to fall armyworm, was further used in indoor experiments to explore its attractant effect on newly hatched larvae of fall armyworm. This experiment was conducted in a 25 cm diameter petri dish. Fresh corn leaves were placed at both ends of the petri dish. One end was placed with a filter paper dripped with 10 μL / mL β-caryophyllene (treatment I), and the other end was used as a blank control (treatment II) ( Fig.10 ). Five newly hatched larvae were placed in the center of the culture dish each time and placed in a ventilated place. The larvae were observed and recorded once 1 h, 2 h and 4 h after release. Each treatment was repeated 12 times.
[0112] The results of the indoor test on β-caryophyllene to attract newly hatched larvae of fall armyworm showed (Table 5) that 10μL / mL of β-caryophyllene had a certain attracting effect on the feeding of fall armyworm larvae. Judging from the average number of larvae counted in each treatment at different time intervals, after 1 hour of release, the newly hatched larvae showed a significantly higher tendency to the corn leaves on the side with β-caryophyllene added than on the side with blank control. When the release time was extended to 2 hours, the number of larvae attracted increased. As time went on, after 4 hours of release, the number of larvae on both sides tended to stabilize. It is speculated that the added plant volatile β-caryophyllene has completely volatilized at this time, and the smell on both sides tends to be consistent.
[0113] Table 5 The attractant effect of single plant volatile components on female Spodoptera frugiperda
[0114] Processing time Average number of larvae / head in treatment I Average number of larvae / head in treatment II 1h 3.16±0.83a 1.08±1.08b 2h 3.50±0.90a 1.42±0.90b 4h 3.50±1.24a 1.50±1.24b
[0115] 6. Field baiting test
[0116] β-Caryophyllene, a compound with good attractant activity for female fall armyworms, was further used in field trials to explore the attractant effect on egg-laying of adult fall armyworms under field conditions. The field trial was conducted in the experimental field of the plantation of Anhui University of Science and Technology from June to August 2022. There were 2 treatments (experimental group and control group) in this field trial, with 12 replicates in each treatment. In each area, the treatment groups were 1m apart and arranged in a checkerboard shape to release the adults of fall armyworm and hang the lures. 2mL of β-Caryophyllene (10μL / mL) was added to each lure in the experimental group, and blank lures ( Fig.11 The number of egg masses and egg grains produced on corn plants in each area during the peak period of egg laying was counted. SPSS21.0 was used to perform one-way analysis of variance on the statistical data of each treatment, and the data were presented in the form of mean and standard error. P < 0.05 was considered to be significantly different.
[0117] The results of the field test of β-caryophyllene to attract fall armyworm showed (Table 6) that β-caryophyllene had a good attractant effect on the oviposition of fall armyworm adults, and the test formula had good potential for attracting fall armyworm in the field. From the statistical average number of egg masses and eggs, the difference in the number of eggs laid by female fall armyworms on corn plants without attractants and corn plants with 10μL / mL β-caryophyllene reached a significant level. The plant volatile β-caryophyllene not only affects the host search behavior of fall armyworm, but also may interfere with its oviposition positioning behavior.
[0118] Table 6 The attractant effect of single plant volatile components on female Spodoptera frugiperda
[0119] Compound Average number of egg masses Average number of eggs Comparison 2.50±1.45b 229.00±155.99b β-Caryophyllene 8.42±2.64a 851.33±383.39a
[0120] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.
Claims
1. A fall armyworm attractant, characterized in that: The fall armyworm attractant comprises an odor compound, and the odor compound is any one of β-caryophyllene, α-pinene, cis-3-hexene-1-ol and 6-methyl-5-heptene-2-one.
2. The fall armyworm attractant according to claim 1, characterized in that When the odor compound is β-caryophyllene or α-pinene, the concentration of the odor compound in the fall armyworm attractant is 5 to 15 μL / mL.
3. The fall armyworm attractant according to claim 1, characterized in that When the odor compound is cis-3-hexen-1-ol or 6-methyl-5-hepten-2-one, the concentration of the odor compound in the fall armyworm attractant is 0.5 to 3 μL / mL.
4. The fall armyworm attractant according to claim 1, characterized in that The fall armyworm attractant also includes an organic solvent.
5. The fall armyworm attractant according to claim 4, characterized in that: The organic solvent is n-hexane.
6. Use of the fall armyworm attractant according to any one of claims 1 to 5 in attracting fall armyworm.
7. The use according to claim 6, characterized in that The fall armyworm attractant is used for attracting fall armyworm larvae and / or adults.
8. The use according to claim 6, characterized in that: The odor compounds in the fall armyworm attractant have strong binding ability with odor binding proteins, and the odor binding proteins include SfruOBP20 and SfruOBP29.
9. The use according to claim 6, characterized in that: The odorant binding protein SfruOBP20 and the odorant binding protein SfruOBP29 are highly expressed in the antennae of Spodoptera frugiperda adults.
10. Use of the fall armyworm attractant according to any one of claims 1 to 5 in attracting female adults of the fall armyworm to lay eggs.