Application of Burkholderia sp. SX-J6 or microbial inoculum containing Burkholderia sp. SX-J6 in saline-alkali environment

Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to application of Burkholderia sp. SX-J6 or a microbial agent containing the Burkholderia sp. SX-J6 in a saline-alkali environment. The invention provides an application of the burkholderia sp. SX-J6 or a bacterial agent containing the burkholderia sp. SX-J6 in a saline and alkaline environment. The preservation number of the burkholderia sp. SX-J6 is CGMCC (China General Microbiological Culture Collection Center) NO.31723; the application comprises promotion of seed germination in the saline-alkali environment and / or promotion of plant growth in the saline-alkali environment. The Burkholderia sp. SX-J6 provided by the invention can promote the germination rate and / or germination length of seeds (such as corn seeds) in the saline-alkali environment, promote the growth of plants in the saline-alkali environment, especially promote the root growth of the plants in the saline-alkali environment, and improve the plant height, chlorophyll content and biomass of the plants in the saline-alkali environment.

Description

Technical Field

[0001] The invention belongs to the technical field of microorganisms, and particularly relates to application of Burkholderia SX-J6 or a bacterial agent containing Burkholderia SX-J6 in a saline-alkali environment. Background Art

[0002] Saline-alkali soil often leads to soil structure deterioration, nutrient imbalance and crop growth disorders due to high salt concentration and alkalinity, which seriously affects the yield and quality of crops. Photovoltaic power stations are built on salinized land. Severe soil salinization restricts the vegetation restoration effect in the power station area. The salinized soil corrodes the photovoltaic support pile foundation, seriously affecting the service life of the photovoltaic support pile foundation. Therefore, ecological restoration of saline-alkali land in photovoltaic areas and improving the salinity of soil in the area are one of the effective ways to achieve the dual carbon goals of photovoltaic new energy.

[0003] The normal growth of plants in saline-alkali land is a key link in the ecological restoration of saline-alkali land. Existing saline-alkali soil improvement technologies are mostly concentrated on the research of traditional agricultural land, including the use of chemical improvers, physical improvement methods, etc. However, the existing saline-alkali soil improvement has problems such as limited improvement effect, poor adaptability and high cost. Microorganisms are the bridge between plants and soil. Salt-alkali tolerant microorganisms can survive in a high saline-alkali environment and help promote plant nutritional growth, improve photosynthesis efficiency, and enhance the ability to resist salt and alkali stress by secreting growth-promoting substances, thereby improving saline-alkali soil and improving the ecological restoration capacity of soil in photovoltaic areas. Therefore, it is particularly important to screen microorganisms that are suitable for living in saline-alkali soils and have the function of secreting growth-promoting substances. Summary of the invention

[0004] The purpose of the present invention is to provide application of Burkholderia SX-J6 or a bacterial agent containing Burkholderia SX-J6 in a saline-alkali environment to promote seed germination in the saline-alkali environment and promote plant growth in the saline-alkali environment.

[0005] The present invention provides Burkholderia ( Burkholderia sp. ) or the application of bacterial agents containing Burkholderia SX-J6 in saline-alkali environments; The deposit number of the Burkholderia SX-J6 is CGMCC NO.31723; The application includes promoting seed germination in saline-alkali environment and / or promoting plant growth in saline-alkali environment.

[0006] Preferably, the concentration of Burkholderia SX-J6 in the bacterial agent is 1×10 6 ~5×10 8 CFU / mL.

[0007] Preferably, the promoting germination of seeds in a saline-alkali environment includes increasing the germination rate and / or germination length of seeds in a saline-alkali environment.

[0008] Preferably, the promoting the growth of plants in saline-alkali environments includes one or more of increasing the plant height of plants in saline-alkali environments, increasing the chlorophyll content of plants in saline-alkali environments, increasing the biomass of plants in saline-alkali environments, and promoting the root growth of plants in saline-alkali environments.

[0009] Preferably, the basic physical and chemical properties of the saline-alkali environment are: bulk density ≥ 1.35 g / cm 3 The average water content is 33.2%, the pH value is 9.86~10.51, and the cationic components of soluble salts include Na + , Fe 2+ , Ca 2+ Mg 2+ , K + , Mn 2+ 、Zn 2+ and Cu 2+ , total salt content is 8108.43μg / g.

[0010] Preferably, the seeds comprise corn seeds.

[0011] Preferably, the plant comprises corn.

[0012] The present invention also provides a method for promoting the growth of plants in a saline-alkali environment, comprising: treating plants grown in a saline-alkali environment with Burkholderia SX-J6 or a bacterial agent containing Burkholderia SX-J6; The deposit number of the Burkholderia SX-J6 is CGMCC NO.31723.

[0013] The present invention also provides a method for promoting seed germination in a saline-alkali environment, comprising: soaking the seeds in a fungal agent containing Burkholderia SX-J6 or Burkholderia SX-J6 and then culturing the seeds in a saline-alkali environment; The deposit number of the Burkholderia SX-J6 is CGMCC NO.31723.

[0014] Preferably, the light-dark ratio of the culture is 12h / 12h, the daytime temperature is 30°C, the light intensity is 15000Lux, and the relative humidity is 80%; the dark temperature is 25°C, and the relative humidity is 75%.

[0015] Beneficial effects: The present invention provides the use of Burkholderia SX-J6 or a bacterial agent containing Burkholderia SX-J6 in a saline-alkali environment; the deposit number of the Burkholderia SX-J6 is CGMCC NO.31723; the use includes promoting seed germination in a saline-alkali environment and / or promoting plant growth in a saline-alkali environment. The Burkholderia SX-J6 provided by the present invention can promote the germination rate and / or germination length of seeds (such as corn seeds) in a saline-alkali environment, promote the growth of plants in a saline-alkali environment, especially promote the root growth of plants in a saline-alkali environment, and increase the plant height, chlorophyll content and biomass of plants in a saline-alkali environment.

[0016] Biological deposit information Burkholderia SX-J6, classified as Burkholderia Burkholderia sp. , deposited on August 23, 2024 at the General Microbiology Center of China Culture Collection Administration, address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Postal Code: 100101, and the deposit number is CGMCC NO.31723. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments are briefly introduced below.

[0018] Figure 1 This is the colony morphology of SX-J6; Figure 2 This is the phylogenetic tree of Burkholderia SX-J6; Figure 3 The germination of corn seeds under different treatment conditions in Example 2; Figure 4 is the length of corn germination under different treatment conditions in Example 2; wherein *** indicates p <0.001; Figure 5 The number of corn roots under different treatment conditions in Example 2; *** indicates p <0.001; Figure 6 is the total length of corn roots under different treatment conditions in Example 2; wherein *** indicates p <0.001; Figure 7 The germination length of corn under different treatment conditions in Example 3; *** indicates p <0.001; Figure 8 The number of corn roots under different treatment conditions in Example 3; *** indicates p <0.001; Fig. 9is the total length of corn roots under different treatment conditions in Example 3; where *** indicates p <0.001; Fig.10 The figures are the actual pictures of corn growth under different treatment conditions in Example 4; among them, a, b and c are the actual pictures after 29 days of treatment; a~c are the control group, the low concentration bacterial solution treatment group and the high concentration bacterial solution treatment group from left to right respectively.

[0019] Fig.11 The height of corn plants under different treatment conditions in Example 4; *** indicates p <0.001; Fig.12 The biomass of corn plants under different treatment conditions in Example 4; where * indicates p <0.05; Fig.13 The relative chlorophyll content of corn plants under different treatment conditions in Example 4; where ** indicates p <0.01, *** indicates p <0.001. DETAILED DESCRIPTION

[0020] The present invention provides the use of Burkholderia SX-J6 or a bacterial agent containing Burkholderia SX-J6 in a saline-alkali environment; the preservation number of the Burkholderia SX-J6 is CGMCC NO.31723; the application includes promoting seed germination in a saline-alkali environment and / or promoting plant growth in a saline-alkali environment. The Burkholderia SX-J6 described in the present invention is a strain isolated from the soil between fixed plates in a large-scale centralized photovoltaic field in Daqing, Heilongjiang Province. The colony shape produced by the Burkholderia SX-J6 on a solid culture medium is round or approximately round, the colony is relatively convex, the texture is uniform, opaque, the surface is smooth, moist, shiny, and grayish white, and the colony edges are neat ( Figure 1 ). The nucleotide sequence of 16S rRNA of Burkholderia SX-J6 of the present invention is shown in SEQ ID NO: 1, which has the function of promoting seed germination in saline-alkali environment and promoting plant growth in saline-alkali environment.

[0021] As an embodiment, the concentration of Burkholderia SX-J6 in the bacterial agent of the present invention is 1×10 6 ~5×10 8 CFU / mL; As another embodiment, the concentration of Burkholderia SX-J6 in the bacterial agent of the present invention is 5×10 6 ~1×10 8 CFU / mL; As another embodiment, the concentration of Burkholderia SX-J6 in the bacterial agent of the present invention is 1×10 7 ~5×10 7CFU / mL.

[0022] As an embodiment, the present invention promotes germination in a saline-alkali environment including increasing the germination rate and / or germination length of seeds in a saline-alkali environment. As an embodiment, the present invention promotes plant growth in a saline-alkali environment including increasing the plant height of plants in a saline-alkali environment, increasing the chlorophyll content of plants in a saline-alkali environment, increasing the biomass of plants in a saline-alkali environment, and promoting the root growth of plants in a saline-alkali environment; as another embodiment, the present invention promotes plant growth in a saline-alkali environment including increasing the plant height of plants in a saline-alkali environment, increasing the chlorophyll content of plants in a saline-alkali environment, increasing the biomass of plants in a saline-alkali environment, and promoting the root growth of plants in a saline-alkali environment.

[0023] As an implementation method, the basic physical and chemical properties of the saline-alkali environment are: bulk density ≥ 1.35 g / cm 3 The average water content is 33.2%, the pH value is 9.86~10.51, and the cationic components of soluble salts include Na + , Fe 2+ , Ca 2+ Mg 2+ , K + , Mn 2+ 、Zn 2+ and Cu 2+ As an embodiment, the soil bulk density of the saline-alkali environment at a soil depth of 0 to 5 cm is 1.35 to 1.45 g / cm 3 , 5~40cm soil depth soil bulk density>1.55g / cm 3 , 40~100cm depth soil bulk density>1.45g / cm 3 And ≤1.55g / cm 3 .

[0024] As an embodiment, the seeds of the present invention include corn seeds. As an embodiment, the plants of the present invention include corn. The present invention is described in the specific embodiments using corn as an example, but it cannot be understood as the entire protection scope of the present invention.

[0025] The present invention also provides a method for promoting plant growth in a saline-alkali environment, comprising: treating plants grown in a saline-alkali environment with Burkholderia SX-J6 or a bacterial agent containing Burkholderia SX-J6; the preservation number of the Burkholderia SX-J6 is CGMCC NO.31723.

[0026] As an embodiment, the treatment method includes irrigation. As an embodiment, the watering is performed once every 5 to 10 days, and 50 to 70 mL / kg of soil is irrigated each time; as another embodiment, the watering is performed once every 7 days, and 60 mL / kg of soil is irrigated each time. As an embodiment, the number of waterings is 3 to 6 times; as another embodiment, the number of waterings is 4 times. The present invention utilizes the bacterial agent described in the above technical solution to irrigate saline-alkali soil in a saline-alkali environment, which can promote the growth of plants in saline-alkali environments, especially promote the plant height of plants in saline-alkali environments, increase the chlorophyll content of plants in saline-alkali environments, increase the biomass of plants in saline-alkali environments, and promote the root growth of plants in saline-alkali environments.

[0027] The present invention also provides a method for promoting seed germination in a saline-alkali environment, comprising: soaking seeds in Burkholderia SX-J6 or a bacterial agent containing Burkholderia SX-J6 and then culturing in a saline-alkali environment; the Burkholderia SX-J6 has a preservation number of CGMCC NO.31723.

[0028] The present invention has no strict requirements on the soaking time, and the soaking can be stopped after the seeds absorb water and swell. As an embodiment, the light-dark ratio of the culture of the present invention is (11~13)h / (13~11)h; as another embodiment, the light-dark ratio of the culture of the present invention is 12h / 12h. As an embodiment, the daytime temperature of the culture is 28~31.5℃, the light intensity is 12000~20000Lux, and the relative humidity is 74%~93%; as another embodiment, the daytime temperature of the culture is 30℃, the light intensity is 15000Lux, and the relative humidity is 80%. As an embodiment, the dark temperature of the culture is 24.4~25.6℃, and the relative humidity is 68%~83%; as another embodiment, the dark temperature of the culture is 25℃, and the relative humidity is 75%. The present invention uses the above-mentioned technical solution to soak the seeds and then culture them, which can promote the germination rate and germination length of seeds in saline-alkali environments.

[0029] In order to further illustrate the present invention, the application of Burkholderia SX-J6 or a bacterial agent containing Burkholderia SX-J6 provided by the present invention in a saline-alkali environment is described in detail below in conjunction with the accompanying drawings and examples, but they should not be construed as limiting the scope of protection of the present invention.

[0030] The culture medium used in the embodiment of the present invention is composed as follows: Solid medium without organic carbon source: Na 2 HPO 4 0.5 g / L, KH 2 PO 4 0.5 g / L, MgSO4 0.3 g / L, CaCl 2 0.2 g / L, NaHCO 3 0.5 g / L, NH 4 Cl 0.5 g / L, NaNO 3 0.25 g / L, NaCl 0.4 g / L, trace element solution 2 mL / L and agar 20 g / L, pH 7.0; the trace element solution composition is: FeCl 2 0.3 g / L, FeSO 4 7H 2 O0.3 g / L, MnSO 4 ·H 2 O 0.15 g / L, ZnSO 4 7H 2 O 0.25 g / L and CoCl 2 0.2 g / L, dilute to 1 L and sterilize through 0.22 μm filter membrane; LB solid medium: 10 g of tryptone, 5 g of yeast extract, 10 g of sodium chloride (NaCl), 15 g of agar powder, dilute to 1000 mL with deionized water, natural pH is 7, stir evenly, sterilize at 121°C for half an hour, and cool to room temperature after sterilization; LB liquid culture medium: 10 g of tryptone, 5 g of yeast extract, 10 g of sodium chloride (NaCl), dilute to 1000 mL with deionized water, natural pH is 7, stir evenly, sterilize at 121°C for half an hour, and cool to room temperature after sterilization.

[0031] Example 1 Isolation and identification of strains 1. Collect soil from the surface layer of 0-20 cm between fixed panels in a centralized large-scale photovoltaic field in Daqing City, Heilongjiang Province. Take 10.0 g of the collected soil, add it to 100 mL of sterilized water, and place it on a shaker for shaking (180 rpm, 30 min) to make a soil suspension. Then dilute the soil suspension in a 10-fold gradient to 10 -5 times, take 0.2 mL of the soil dilution, apply it to the solid culture medium without organic carbon source, and culture it upside down at 30℃ for 4 days.

[0032] 2. Select the colonies with good morphology, growth potential and fast growth rate in the solid medium without organic carbon source, purify them on the new solid medium without organic carbon source by inoculation loop streak method for multiple times until pure culture, and screen out a target strain, numbered SX-J6, with round or nearly round colonies, light yellow, smooth, moist, shiny surface, neat edges, and different sizes ( Figure 1 ).

[0033]

[0034] 4. Using the NCBI database, BLAST analysis was performed based on the 16S rRNA gene sequence of strain SX-J6, and a phylogenetic tree was constructed. The results showed that strain SX-J6 was Burkholderia paludis MSh1 The 16S rRNA gene sequence of Burkholderia contaminans J2956 The homology of the 16S rRNA gene sequence of Burkholderia lata 383 The 16S rRNA gene sequence of Burkholderia arboris R-24201 The 16S rRNA gene sequence of the genus NR_042634.1 is 99.78% homologous to Burkholderia aenigmatica LMG 13014 The homology of the 16SrRNA gene sequence of strain SX-J6 (accession number NR_174230.1) is 99.64%. Burkholderia paludis and Burkholderia contaminans The closest phylogenetic relationship ( Figure 2 ), combined with its bacterial morphology and colony characteristics ( Figure 1 ), the strain was identified as Burkholderia ( Burkholderia sp. ), named Burkholderia Burkholderia sp. )SX-J6 and made a biological deposit.

[0035] Example 2 Effects of Burkholderia SX-J6 on germination and growth of corn in saline-alkali environment 1. Preparation of Burkholderia SX-J6 bacterial suspension The Burkholderia SX-J6 isolated in Example 1 was picked up and inoculated into 10 mL of LB liquid medium (tryptone 10 g / L, yeast extract 5 g / L and sodium chloride 10 g / L, pH 7.00), and cultured at 35 ° C, 180 rpm and shaking for 48 h to obtain SX-J6 seed solution; the concentration of SX-J6 seed solution was adjusted to 5×10 7 CFU / mL and 5×10 8 CFU / mL, and obtained different concentrations of Burkholderia SX-J6 bacterial suspension.

[0036] 2. Corn seed treatment: Select corn seeds with full grains, no mildew, uniform size, no wormholes, and no damage, rinse the surface dust with sterile water, wipe with 75% v / v ethanol for 16~20s, then soak in 0.1wt.% potassium permanganate solution for 10min, and finally rinse with sterile water 5~8 times. Take 100mL of the sterile water from the last rinse and spread it on the peptone solid culture medium, invert and culture at a constant temperature for 48h, observe whether there is colony formation on the surface of the culture medium to verify the thoroughness of corn surface disinfection, and set aside.

[0037] 3. The treated seeds were randomly divided into 4 treatment groups, each with 3 replicates. Each replicate used 50 corn seeds and the following treatments were performed: Control (RO water) group: The treated corn seeds were soaked in sterile water at room temperature; Saline-alkali solution group: The treated corn seeds were immersed in a solution with a pH value of 10 and a salinity of 18% at room temperature; Low concentration J6 bacterial solution + saline-alkali solution group: pH 10, salinity 18% solution and 5×10 7 CFU / mL of Burkholderia SX-J6 bacterial suspension was mixed in equal volumes to obtain a mixed solution; the treated corn seeds were immersed in the mixed solution at room temperature; High concentration J6 bacterial solution + saline-alkali solution group: pH 10, salinity 18% solution and 5×10 8 The Burkholderia SX-J6 bacterial suspensions with CFU / mL were mixed in equal volumes to obtain a mixed solution; the treated corn seeds were immersed in the mixed solution at room temperature.

[0038] After the seeds of each treatment group absorb water and swell, use clean tweezers to evenly place the treated corn seeds on the moist filter paper, and then place them in the culture pot, and then gently cover the seeds with another layer of moist filter paper, moisten the filter paper with distilled water to ensure that it is evenly moist but not waterlogged, cover it on top of the seeds, and place it in a plant growth incubator. Set the daytime temperature to 30°C, the light intensity to 10,000~15,000Lux, the night temperature to 25°C, and the air humidity to 75% to maintain humidity and sufficient air, and place it in an oxygen-sufficient environment. During the culture period, ensure that the filter paper is moist, and compare and observe the growth indicators of corn seeds with different treatments such as germination rate, bud length, number of roots, and root length every day, and record the seed germination process indicators. Statistical tests were performed to determine whether there were significant differences in corn germination rate and bud length between different experimental groups. After data analysis, R language (R v4.1.2) was used to draw the graph. The results are shown in the figure. Figures 3 to 6 shown.

[0039] according to Figure 3It can be seen that within 5 days, the germination and growth of corn under saline-alkali solution stress were poor. The growth of corn in the low-concentration J6 bacterial solution + saline-alkali solution group was significantly better than that in other treatments, and the growth of corn in the high-concentration J6 bacterial solution + saline-alkali solution group was better than that under saline-alkali solution stress conditions. Comparing the germination and growth of corn seeds in different treatment groups, the germination rate of corn seeds under saline-alkali solution conditions was the lowest, indicating that the saline-alkali solution inhibited the germination of corn seeds, while the low-concentration J6 bacterial solution (5×10 7 CFU / mL) + saline solution, high concentration J6 bacterial solution (5×10 8 CFU / mL) + saline-alkali solution can significantly increase the germination rate and total germination rate of corn compared with saline-alkali solution ( Figure 3 ). Comparing the bud growth of corn seeds in different treatment groups, the bud growth level was the lowest under the saline-alkali solution, indicating that the saline-alkali solution inhibited the growth of corn buds, while the low concentration of J6 bacterial solution (5×10 7 CFU / mL) + saline solution, high concentration J6 bacterial solution (5×10 8 CFU / mL) + saline-alkali solution, compared with saline-alkali solution, the sprout length showed a significant increase, and Burkholderia SX-J6 had a growth-promoting effect ( Figure 4 ).

[0040] The root numbers of corn seeds after germination in different treatment groups were compared. The number of roots in saline-alkali solution was the least, indicating that saline-alkali solution inhibited the formation, elongation and branching of corn roots, while low concentration of J6 bacterial solution (5×10 7 CFU / mL) + saline solution, high concentration J6 bacterial solution (5×10 8 CFU / mL) + saline-alkali solution, compared with the saline-alkali solution, the root number showed a significant increase, indicating that the Burkholderia SX-J6 bacterial solution has the ability to promote the root formation of corn seeds under saline-alkali conditions ( Figure 5 ).

[0041] The root length of corn seeds after germination in different treatment groups was compared. The root length was the shortest under saline-alkali solution, indicating that saline-alkali solution inhibited the growth of corn roots, while low concentration J6 solution (5×10 7 CFU / mL) + saline solution, high concentration J6 bacterial solution (5×10 8 CFU / mL) + saline-alkali solution, compared with the saline-alkali solution, the total root length showed a significant increase, indicating that Burkholderia SX-J6 bacterial solution has the ability to promote the root growth of corn seeds under saline-alkali conditions ( Figure 6 ).

[0042] Example 3 Effects of Burkholderia SX-J6 on the Growth of Maize Roots 1. Randomly divide the corn seeds processed in Example 2 into 3 treatment groups, with 3 replicates in each group. Use 50 corn seeds for each replicate and conduct the following treatments: Group without adding bacterial solution: Soak the processed corn seeds in sterile water at room temperature; Group adding appropriate concentration of bacterial solution: Soak the processed corn seeds in the Burkholderia sp. SX-J6 bacterial suspension with a concentration of 5×10 7 CFU / mL obtained in Example 2 at room temperature; Group adding high concentration of bacterial solution: Soak the processed corn seeds in the Burkholderia sp. SX-J6 bacterial suspension with a concentration of 5×10 8 CFU / mL obtained in Example 2 at room temperature.

[0043] 2. After the seeds in each treatment group absorb water and swell, use clean forceps to evenly place the processed corn seeds on moist filter paper, then place them in a culture basin, and then gently cover the seeds with another layer of moist filter paper. Moisten the filter paper with distilled water to ensure it is evenly moist but without water accumulation, cover it above the seeds, and place it in a plant growth incubator. Set the daytime temperature at 30°C, the light intensity at 10000 - 15000 Lux, the nighttime temperature at 25°C, and the air humidity at 75% to maintain humidity and sufficient air. Ensure sufficient oxygen. During the cultivation period, keep the filter paper moist. After culturing for 5 days, observe the germination length, root number, and root length of the corn seeds under different treatments. After analyzing the data, use R language (R v4.1.2) for plotting. The results are as shown in Figure 7 、 8 and 9.

[0044] According to Figure 7 it can be seen that by comparing the average germination length of the experimental corn in different treatment groups, soaking the seeds with an appropriate concentration of SX-J6 bacterial solution, the average germination length of the corn is significantly greater than that of the control group, while soaking the seeds with a high concentration of SX-J6 bacterial solution, the average germination length of the corn is significantly less than that of the control group. This shows that an appropriate concentration of SX-J6 bacterial solution helps the growth of corn root length. According to Figure 8 it can be seen that by comparing the average root number of the experimental corn in different treatment groups, soaking the seeds with an appropriate concentration of SX-J6 bacterial solution, the average root number of the corn is significantly greater than that of the control group, while soaking the seeds with a high concentration of SX-J6 bacterial solution, the average root number of the corn is significantly less than that of the control group. This shows that an appropriate concentration of SX-J6 bacterial solution helps the formation of corn roots. According to Fig. 9 it can be seen that by comparing the average total root length of the experimental corn in different treatment groups, soaking the seeds with an appropriate concentration of SX-J6 bacterial solution, the average total root length of the corn is significantly greater than that of the control group, while soaking the seeds with a high concentration of SX-J6 bacterial solution, the average total root length of the corn is significantly less than that of the control group. This shows that an appropriate concentration of SX-J6 bacterial solution helps the growth of corn root length.

[0045] Example 4 Effects of Burkholderia SX-J6 on the Vegetative Growth of Maize 1. Preparation of Burkholderia SX-J6 bacterial suspension The Burkholderia SX-J6 isolated in Example 1 was selected and inoculated into 10 mL of LB liquid medium (tryptone 10 g / L, yeast extract 5 g / L and sodium chloride 10 g / L, pH 7.0), and cultured at 35 ° C, 180 rpm for 48 h to obtain SX-J6 seed solution; the concentration of SX-J6 seed solution was adjusted to 5×10 7 CFU / mL and 5×10 8 CFU / mL, and obtained different concentrations of Burkholderia SX-J6 bacterial suspension.

[0046] 2. Soil collection and processing: On July 19, 2024, at the Daqing Base, a national photovoltaic and energy storage demonstration experimental platform in Daqing, Heilongjiang, natural salinized soil was collected. The soil sampling depth was 0-20cm, and the soil bulk density was measured to be between 1.35-1.55g / cm 3 The average soil moisture content in the photovoltaic field is 33.2%, the soil pH is between 9.86 and 10.51, and the main cation components of soluble salts include Na + , Fe 2+ , Ca 2+ Mg 2+ , K + , Mn 2+ 、Zn 2+ and Cu 2+ The total salt content is 8108.43 μg / g. The collected soil is naturally air-dried for about a week and sieved to remove non-soil impurities.

[0047] 3. Corn seed treatment: Select corn seeds (Baiyu Nuo 909) with full grains, no mildew, uniform size, no wormholes, and no damage, rinse the surface dust with sterile water, wipe with 75% v / v ethanol for 16~20s, then soak in 0.1wt.% potassium permanganate solution for 10min, and finally rinse with sterile water 5~8 times. Take 100mL of the sterile water from the last rinse and spread it on the peptone solid culture medium, invert and culture at a constant temperature for 48h, observe whether there is colony formation on the surface of the culture medium to verify the thoroughness of corn surface disinfection, and set aside.

[0048] 4. Use the saline-alkali soil, peat soil, and perlite sieved in step 2 as the soil matrix together. First, fill a 0.4L flower pot with the saline-alkali soil, sow the treated corn seeds, and then cover with peat soil and perlite in sequence. Each flower pot contains 2 kg of soil matrix, and the volume ratio of saline-alkali soil, peat soil, and perlite is 4.0:0.5:0.5. Randomly divide them into a low-concentration J6 bacterial liquid group, a high-concentration J6 bacterial liquid group, and a control group, with 3 replicates in each group, and conduct the following treatments: Control group: Water 30 mL of pure water (RO water) on the sowing day, and then water 30 mL of pure water near the roots of the corn plants every 7 days; Low-concentration J6 bacterial liquid group: Water 30 mL of the Burkholderia sp. SX-J6 bacterial suspension with a concentration of 5×10 7 CFU / mL obtained in step 1 on the sowing day, and then water 30 mL near the roots of the corn plants every 7 days; High-concentration J6 bacterial liquid group: Water 30 mL of the Burkholderia sp. SX-J6 bacterial suspension with a concentration of 5×10 8 CFU / mL obtained in step 1 on the sowing day, and then water 30 mL near the roots of the corn plants every 7 days.

[0049] Place each treatment group in a plant incubator for cultivation, set the daytime temperature at 30°C, light at 15000 Lux for 12 h, and relative humidity at 80%; the dark temperature is 12 h, the temperature is set at 25°C, and the relative humidity is 75%.

[0050] 5. On the 15th day, 22nd day, and 29th day of the treatments of each treatment group in step 4, observe the growth of the corn and measure and record the plant height of the corn (the average plant height on the 15th day, 22nd day, and 29th day), and conduct statistical tests to determine whether there are significant differences in plant height among different experimental groups. After analyzing the data, use R language (R v4.1.2) for plotting. The results show that the growth of the corn watered with the low-concentration bacterial liquid is the best. The growth of the corn in the control group is worse than that in the low-concentration bacterial liquid treatment group, but better than that in the high-concentration bacterial suspension treatment group, indicating that the Burkholderia sp. SX-J6 bacterial liquid with a concentration of 5×10 7 CFU / mL can promote the growth and development of corn in saline-alkali soil ( Fig.11 ). Under the treatment method of the low-concentration bacterial liquid (5×10 7 CFU / mL), the average height of the corn plants is 15.63 cm, which is significantly higher than the height of the corn plants under the treatment method of the control group (10.85 cm).

[0051] 6. Step 4 After 28 days of treatment, the relative chlorophyll content of corn was measured using a plant nutrition meter. After 30 days, the corn plants were taken out and the fresh weight of the plants was weighed. Statistical tests were performed to determine whether there were significant differences in the relative chlorophyll content and biomass (fresh weight) of corn between different experimental groups. After data analysis, R language (R v4.1.2) was used to draw the graph. The results showed that at low concentrations of bacterial solution (5×10 7 CFU / mL) treatment, the biomass of corn plants was 6.82g, which was significantly higher than the biomass of corn plants in the control group (4.96g) ( Fig.12 ); in low concentration bacterial solution (5×10 7 CFU / mL) treatment, the relative chlorophyll content of corn plants was 34.49, which was significantly higher than the relative chlorophyll content of corn plants in the control group (28.15 ( Fig.13 ).

[0052] Based on the above content, it can be seen that the Burkholderia SX-J6 provided by the present invention can promote the germination rate and / or germination length of seeds in a saline-alkali environment, promote the growth of plants in a saline-alkali environment, especially promote the root growth of plants in a saline-alkali environment, and increase the plant height, chlorophyll content and biomass of plants in a saline-alkali environment.

[0053] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. Burkholderia Burkholderia sp. ) Application of SX-J6 or bacterial agents containing Burkholderia SX-J6 in saline-alkali environments; The deposit number of the Burkholderia SX-J6 is CGMCC NO.31723; The application includes promoting seed germination in saline-alkali environment and / or promoting plant growth in saline-alkali environment.

2. The use according to claim 1, characterized in that: The concentration of Burkholderia SX-J6 in the bacterial agent is 1×10 6 ~5×10 8 CFU / mL.

3. The use according to claim 1, characterized in that: The promoting germination of seeds in saline-alkali environment includes increasing the germination rate and / or germination length of seeds in saline-alkali environment.

4. The use according to claim 1, characterized in that: The promoting the growth of plants in saline-alkali environments includes one or more of increasing the plant height of plants in saline-alkali environments, increasing the chlorophyll content of plants in saline-alkali environments, increasing the biomass of plants in saline-alkali environments, and promoting the root growth of plants in saline-alkali environments.

5. The use according to claim 1, characterized in that: The basic physical and chemical properties of the saline-alkali environment are: bulk density is 1.35~1.55g / cm 3 The average water content is 33.2%, the pH value is 9.86~10.51, and the cationic components of soluble salts include Na + , Fe 2+ , Ca 2+ Mg 2+ , K + , Mn 2+ 、Zn 2+ and Cu 2+ , total salt content is 8108.43μg / g.

6. The use according to claim 1, characterized in that: The seeds include corn seeds.

7. The use according to claim 1, characterized in that: The plants include corn.

8. A method for promoting plant growth in a saline-alkali environment, characterized in that: include: Treating plants grown in saline-alkali environments with Burkholderia SX-J6 or a bacterial agent containing Burkholderia SX-J6; The deposit number of the Burkholderia SX-J6 is CGMCC NO.31723.

9. A method for promoting seed germination in a saline-alkali environment, characterized in that: include: The seeds are soaked in Burkholderia SX-J6 or a fungal agent containing Burkholderia SX-J6 and then cultured in a saline-alkali environment; The deposit number of the Burkholderia SX-J6 is CGMCC NO.31723.

10. The method according to claim 9, characterized in that The light-dark ratio of the culture is 12h / 12h, the daytime temperature is 30°C, the light intensity is 15000Lux, and the relative humidity is 80%; the dark temperature is 25°C, and the relative humidity is 75%.

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