Composition containing plant essential oil and benzyl isothiocyanate, preparation and application

Abstract

The invention discloses a composition containing plant essential oil and benzyl isothiocyanate, a preparation and application, effective components comprise an active component A and an active component B. The active component A is benzyl isothiocyanate, and the active component B is selected from one or more of lemon grass oil, eugenol, schizonepeta oil, verbena oil and peppermint oil. In the composition, the weight ratio of the active component A to the active component B is (1: 20)-(20: 1). The compound agent can be prepared into an oil agent, an emulsion in water, a soluble liquid agent, a microemulsion, granules and the like, and is applied to prevention and treatment of soil nematodes, soil pests and soil-borne pathogenic bacteria. The compound agent is obvious in synergism, high in bacteriostatic and nematicidal activity, small in relative dosage, and especially obvious in fumigation effect on pests such as soil nematodes and soil-borne pathogenic fungi.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticide compounding, and relates to a plant-derived component composition for preventing and controlling harmful organisms in soil (nematodes, soil-borne pathogenic fungi, soil-borne pathogenic bacteria, and soil pests) and its application, specifically to a composition containing plant essential oil and benzyl isothiocyanate, a preparation and its application. Background Art

[0002] Plant parasitic nematodes are widely distributed and diverse around the world. They are one of the main pathogens that endanger agricultural production. According to statistics, there are more than 5,000 species of plant parasitic nematodes in the world, causing losses of more than 173 billion US dollars. Among them, the most serious type of nematode that affects crops is the root-knot nematode. It has been found that the damage caused by root-knot nematodes covers a variety of food crops, cash crops, horticultural seedlings, etc., more than 3,000 species. Among them, the vegetables of the Cruciferae, Solanaceae, Leguminosae and Liliaceae families have the most serious production reduction. In addition to directly causing crop death, root-knot nematodes can also work synergistically with other soil-borne diseases to cause complex infections and aggravate diseases. At the same time, the wounds caused by nematodes when invading plants also open the door for the invasion of bacteria, fungi, and viruses, greatly affecting the quality and yield of agricultural products, and seriously threatening the sustainable development of agriculture.

[0003] The control methods of root-knot nematodes are divided into: chemical control, physical control, agricultural control, genetic engineering breeding and biological control, among which chemical control is the main measure. Traditional chemical control methods mostly rely on fumigation chemical pesticides, among which highly toxic pesticides dominate. This type of pesticide is costly, highly toxic and harmful to the environment. With the improvement of public environmental awareness and the improvement of agricultural product safety standards, highly toxic pesticides are gradually banned and eliminated. At present, there are limited types of control agents for plant nematode diseases on the market, mainly including avermectin, thiazophos, fluopyram, flufenamide and other ingredients. In particular, thiazophos and avermectin and their compound preparations have long been the mainstream of the market. However, the effect of thiazophos is poor when the dosage is low, and it is easy to damage the roots when the dosage is high; avermectin has poor horizontal mobility in the soil and is easily adsorbed by soil particles or degraded by microorganisms, resulting in fluctuations in the control effect and instability.

[0004] Plant resources in nature are extremely abundant and are an important source of natural products. Compared with traditional chemical pesticides, the use of plant extracts to control pests and diseases has many advantages. They are less toxic, easy to decompose, can coexist harmoniously with the environment, and are not easy to induce resistance, which is of great benefit to the healthy growth of plants and biosafety. At present, the most commonly used botanical insecticides in the world include nicotine, aldehyde compounds, ketone compounds, sulfur compounds, triterpenoid compounds, alkaloids, glycosides, flavonoids and various plant essential oils.

[0005] Benzyl isothiocyanate, also known as benzyl isothiocyanate and benzyl mustard oil in Chinese, is a light yellow oily liquid that is widely found in plants of the cruciferous family, especially in plants of the genera Brassica and Horseradish, where the output is considerable. In addition to the seasoning effect, isothiocyanate compounds have been widely studied for their various biological activities, proving that isothiocyanates have the ability to kill nematodes or inhibit bacteria, such as southern root-knot nematodes, pine wood nematodes, Salmonella typhi, Staphylococcus aureus, tuberculosis, Salmonella, diphtheria, Escherichia coli, Bacillus subtilis, etc. It also has a high inhibition rate against fungi such as Paecilomyces variotii and Saccharomyces cerevisiae. As a potential nematicide and fungicide, benzyl isothiocyanate has certain research value and application prospects.

[0006] How to utilize botanical pesticides to improve the effectiveness of plant nematode control for farmers while ensuring higher biosafety is an urgent problem to be solved. Summary of the invention

[0007] The invention aims to provide a composition and a compounding agent of benzyl isothiocyanate and plant essential oil with obvious synergistic effect.

[0008] Another object of the present invention is to provide an application of a compound of benzyl isothiocyanate and plant essential oil in preparing a pesticide fumigant for preventing and controlling soil-borne pathogens and soil nematodes.

[0009] In order to achieve the above object, the technical solution adopted by the present invention is:

[0010] A composition containing plant essential oil and benzyl isothiocyanate, wherein the effective components of the composition are active ingredient A and active ingredient B;

[0011] The active ingredient A is benzyl isothiocyanate;

[0012] The active ingredient B is at least one selected from lemongrass oil, eugenol, nepeta oil, verbena oil and peppermint oil;

[0013] The weight ratio of active ingredient A to active ingredient B is (1-20):(20-1).

[0014] Optionally, the composition comprises: a weight ratio of benzyl isothiocyanate to lemongrass oil of 1:20 to 15:1; preferably 1:1;

[0015] The weight ratio of benzyl isothiocyanate to eugenol is 13:1 to 1:9, preferably 1:5;

[0016] The weight ratio of benzyl isothiocyanate to catmint oil is 9:1 to 1:9, preferably 1:5;

[0017] The weight ratio of benzyl isothiocyanate to verbena oil is 15:1 to 1:9, preferably 1:3;

[0018] The weight ratio of benzyl isothiocyanate to peppermint oil is 20:1 to 1:15, preferably 5:1.

[0019] A composition preparation containing plant essential oil and benzyl isothiocyanate, wherein the preparation contains any one of the compositions containing plant essential oil and benzyl isothiocyanate described in the present invention;

[0020] Calculated by mass percentage, in the preparation, the active ingredient A is 1% to 15%, and the active ingredient B is 1% to 30%.

[0021] Optionally, the preparation is an oil, and the components and weight percentages are:

[0022] Active ingredient A 1% to 15%, active ingredient B 1% to 30%, antioxidant 0.1% to 2%, solvent 10% to 85%, vegetable oil to 100%;

[0023] Wherein, the antioxidant is ascorbic acid or amyl gallate; the solvent is at least one of ethyl formate, 2-butyl acetate, furfural, 5-hydroxymethylfurfural, 5-methylfurfural or ethyl acetate; and the vegetable oil is turpentine or Sichuan dove oil.

[0024] Optionally, the preparation is an aqueous emulsion, and the components and weight percentages are:

[0025] Active ingredient A 1% to 10%, active ingredient B 1% to 35%, emulsifier 12% to 28%, ascorbic acid 0.1% to 2%, solvent 10% to 30%, and water to make up to 100%;

[0026] Wherein, the emulsifier is Nongru 500, 1601, Tween-80, TX-10, OP-10 or Span 80; the solvent is at least one of ethyl formate, 2-butyl acetate, furfural, 5-hydroxymethylfurfural, 5-methylfurfural or ethyl acetate.

[0027] Optionally, the preparation is a soluble liquid, and the components and weight percentages are:

[0028] Active ingredient A 1% to 20%, active ingredient B 1% to 30%, emulsifier 12% to 60%, ascorbic acid 1% to 8%, and solvent to make up to 100%;

[0029] Wherein, the emulsifier is Nongru 500, 1601, Tween-80, TX-10, OP-10 or Span 80; the solvent is at least one of ethyl formate, sec-butyl acetate, furfural, 5-hydroxymethylfurfural, 5-methylfurfural or ethyl acetate.

[0030] Optionally, the preparation is a microemulsion, and the components and weight percentages are:

[0031] Active ingredient A 1% to 15%, active ingredient B 1% to 30%, solvent 10% to 40%, emulsifier 5% to 20%, ascorbic acid 0.1% to 2%;

[0032] Wherein, the emulsifier is Nongru 500, 1601, Tween-80, TX-10, OP-10 or Span 80; the solvent is at least one of ethyl formate, 2-butyl acetate, furfural, 5-hydroxymethylfurfural, 5-methylfurfural or ethyl acetate.

[0033] Optionally, the preparation is a granule, and the components and weight percentages are:

[0034] Active ingredient A 1% to 10%, active ingredient B 1% to 30%, carrier filler 60% to 80%, disintegrant 1% to 3%, binder 1% to 3%, auxiliary agent 0% to 10%;

[0035] The carrier filler is selected from at least one of starch, kaolin, diatomaceous earth and light calcium carbonate; the disintegrant package is selected from at least one of cellulose ether and its derivatives, sodium carboxymethyl starch and bentonite; the binder is selected from at least one of sodium carboxymethyl cellulose, xanthan gum and polyethylene glycol; the auxiliary agent is selected from at least one of glucose, calcium lignin sulfonate and magnesium aluminum silicate.

[0036] Use of any of the compositions containing plant essential oil and benzyl isothiocyanate described in the present invention or any of the composition preparations containing plant essential oil and benzyl isothiocyanate described in the present invention in preparing a fumigant for controlling soil-borne pathogens and / or soil nematodes.

[0037] Optionally, the soil-borne pathogens include Agrobacterium tumefaciens and / or Fusarium wilt;

[0038] The soil nematodes are root-knot nematodes.

[0039] The advantages of the present invention are:

[0040] The compound agent of the invention has obvious synergistic effect, high antibacterial and nematicidal activities, and relatively small dosage, and is particularly effective in fumigating harmful organisms such as soil nematodes and soil-borne pathogenic fungi. DETAILED DESCRIPTION

[0041] The composition containing plant essential oil and benzyl isothiocyanate of the present invention comprises an active ingredient A of benzyl isothiocyanate, an active ingredient B selected from at least one of lemongrass oil, eugenol, nepeta oil, verbena oil and peppermint oil; and a weight ratio of the active ingredient A to the active ingredient B is 1:20 to 20:1.

[0042] The preferred combination is: the weight ratio of benzyl isothiocyanate to lemongrass oil is 1:20 to 20:1; the weight ratio of benzyl isothiocyanate to eugenol is 1:9 to 13:1; the weight ratio of benzyl isothiocyanate to nepeta oil is 1:9 to 9:1; the weight ratio of benzyl isothiocyanate to verbena oil is 1:9 to 15:1; the weight ratio of benzyl isothiocyanate to peppermint oil is 1:15 to 20:1.

[0043] The invention discloses a composition preparation containing plant essential oil and benzyl isothiocyanate, wherein the composition contains the plant essential oil and benzyl isothiocyanate.

[0044] The preparation is an oil preparation, and the components and weight percentages are: active ingredient A 1% to 15%, active ingredient B 1% to 30%, antioxidant 0.1% to 2%, solvent 10% to 85%, and vegetable oil to 100%; wherein the antioxidant is ascorbic acid or amyl gallate; the solvent is one or more of ethyl formate, sec-butyl acetate, furfural, 5-hydroxymethylfurfural, 5-methylfurfural or ethyl acetate; the vegetable oil is turpentine or Chuanqiong oil. The preparation process is: first, the antioxidant is added to the quantitatively weighed mixed mother liquor of benzyl isothiocyanate and plant extract essential oil and mixed thoroughly. Then, the accurately quantitatively weighed vegetable oil and solvent are mixed thoroughly. Finally, the above two solutions are mixed in a preparation tank and stirred continuously for 1 to 3 hours. The product is packaged in double-barrier plastic bottles or aluminum bottles with inner barrier layer and sealed for storage.

[0045] The preparation is a water emulsion, and the components and weight percentages are: active ingredient A 1% to 10%, active ingredient B 1% to 35%, emulsifier 12% to 28%, ascorbic acid 0.1% to 2%, solvent 10% to 30%, and water is used to make up to 100%; wherein, the emulsifier is Nongru 500, 1601, Tween-80, TX-10, OP-10 or Span 80; the solvent is one or more of ethyl formate, acetic acid sec-butyl alcohol, furfural, 5-hydroxymethylfurfural, 5-methylfurfural or ethyl acetate. The preparation process is: first, ascorbic acid is added to the quantitatively weighed mixed mother liquor of benzyl isothiocyanate and plant essential oil and mixed thoroughly. The accurately quantitatively weighed emulsifier and solvent are fully mixed. Finally, the above two solutions are continuously stirred in the preparation tank for 1 to 3 hours. The product is packaged in double-barrier plastic bottles, glass bottles or aluminum bottles with inner barrier layers, and sealed for storage.

[0046] The preparation is a soluble liquid, and the components and weight percentages are: active ingredient A 1% to 20%, active ingredient B 1% to 30%, emulsifier 12% to 60%, ascorbic acid 1% to 8%, and the solvent is made up to 100%; wherein the emulsifier is Nongru 500, 1601, Tween-80, TX-10, OP-10 or Span 80; the solvent is one or more of ethyl formate, acetic acid sec-butyl alcohol, furfural, 5-hydroxymethylfurfural, 5-methylfurfural or ethyl acetate. The preparation process is: firstly, ascorbic acid is added into the mixed mother liquor of quantitatively weighed benzyl isothiocyanate and plant essential oil and fully mixed. The accurately quantitatively weighed agricultural emulsifier and solvent are fully mixed. Finally, the above two solutions are continuously stirred in the preparation tank for 1 to 3 hours. The product is divided into double-barrier plastic bottles and sealed for storage.

[0047] The preparation is a microemulsion, and the components and weight percentages are: active ingredient A 1% to 15%, active ingredient B 1% to 30%, solvent 10% to 40%, emulsifier 5% to 20%, ascorbic acid 0.1% to 2%; wherein the emulsifier is Nongru 500, 1601, Tween-80, TX-10, OP-10 or Span 80; and the solvent is one or more of ethyl formate, acetic acid sec-butyl alcohol, furfural, 5-hydroxymethylfurfural, 5-methylfurfural or ethyl acetate. The preparation process is as follows: benzyl isothiocyanate and plant extract essential oil are fully mixed in a mixing tank (1). Ascorbic acid is added to the mixing tank (1) and fully mixed. An emulsifier is added to the mixing tank (2), and after mixing, the solvent is added and fully mixed. The materials in the mixing tank (2) are all transferred into the mixing tank (1) through a pipeline, and the tank mouth is sealed and a stirrer is started for stirring. The product is packaged into double-barrier plastic bottles, with 500 mL in each bottle.

[0048] The preparation is a granule, and the components and weight percentage are: active ingredient A 1% to 10%, active ingredient B 1% to 30%, carrier filler 60% to 80%, disintegrant 1% to 3%, binder 1% to 3%, auxiliary agent 0% to 10%; the carrier filler is selected from starch, kaolin, diatomaceous earth, light calcium carbonate; disintegrants include cellulose ether and its derivatives, sodium carboxymethyl starch, bentonite; binders include sodium carboxymethyl cellulose, xanthan gum, polyethylene glycol (PEG); auxiliary agents include glucose, calcium lignin sulfonate, magnesium aluminum silicate. One or more of them are selected respectively. The preparation process is: granulation is carried out by extrusion granulation method, first weighing a certain amount of benzyl isothiocyanate and plant essential oil and mixing with carrier filler for adsorption, then adding binder, disintegrant, auxiliary agent, etc. and mixing them evenly, then granulating by extrusion granulator, and putting the prepared granules into a drying oven at 60°C for 6 hours. The qualified granules are packaged with suitable packaging materials, such as aluminum foil bags or plastic bottles.

[0049] The above production processes are all completed by machines under closed conditions, and workers should avoid direct contact with the liquid medicine. Production workers should have complete protective measures.

[0050] The plant-derived component composition of the present invention is used in the preparation of a fumigant for controlling soil-borne pathogens and / or soil nematodes. The plant-derived component composition preparation of the present invention is used in the preparation of a fumigant for controlling soil-borne pathogens and / or soil nematodes.

[0051] The watermelon wilt pathogen (Fusarium oxysporum) and Agrobacterium tumefaciens used in the following experiments are conventional pathogens, which can be isolated and identified by the laboratory itself, or purchased on the market.

[0052] Experimental Example 1: Determination of the indoor fumigation activity of five plant essential oils, benzyl isothiocyanate and their mixtures against root-knot nematodes

[0053] The indoor fumigation activity of five kinds of plant essential oils, benzyl isothiocyanate and its compound agent against root-knot nematodes was determined by in vitro fumigation in an indoor dryer. After fumigation in the dryer, the dead nematodes in the 24-well plate were counted under a microscope with a bottom light source at 24h, 48h, and 72h, respectively. The number of nematodes in each field of view was about 200, and the death of nematodes was observed and recorded.

[0054] The five purchased plant essential oils, benzyl isothiocyanate and their compound agents were prepared with acetone at different concentrations for preliminary screening of their fumigation activity against root-knot nematodes. A total of 10 treatments were set up in the experiment:

[0055] (1) 5 μg / mL lemongrass oil;

[0056] (2) 5 μg / mL eugenol;

[0057] (3) 5 μg / mL catmint oil;

[0058] (4) 5 μg / mL verbena oil;

[0059] (5) 5 μg / mL peppermint oil;

[0060] (6) 5 μg / mL benzyl isothiocyanate

[0061] (7) 5 μg / mL lemongrass oil + 5 μg / mL benzyl isothiocyanate (1:1);

[0062] (8) 5 μg / mL eugenol + 5 μg / mL benzyl isothiocyanate (1:1);

[0063] (9) 5 μg / mL catmint oil + 5 μg / mL benzyl isothiocyanate (1:1);

[0064] (10) 5 μg / mL verbena oil + 5 μg / mL benzyl isothiocyanate (1:1);

[0065] (11) 5 μg / mL peppermint oil + 5 μg / mL benzyl isothiocyanate (1:1);

[0066] Table 1 Fumigation activity of different plant essential oils single and compound preparations against root-knot nematodes

[0067]

[0068]

[0069] Lemongrass oil, eugenol, nepeta oil, verbena oil, and peppermint oil all had certain fumigation activity against root-knot nematodes at a concentration of 5 μg / mL. Among them, the mortality rate of lemongrass oil against root-knot nematodes after 72 hours of fumigation was 99.1%, showing the strongest fumigation activity. After compounding with benzyl isothiocyanate, they all showed synergistic effects. The biological activity of the compound agent was significantly higher than that of the single agent, and the use of benzyl isothiocyanate could be significantly reduced.

[0070] Experimental Example 2: Indoor contact killing activity of plant essential oils, benzyl isothiocyanate and their mixtures against root-knot nematodes. The experiment has 11 treatment groups:

[0071] (1) 25 μg / mL lemongrass oil;

[0072] (2) 25 μg / mL eugenol;

[0073] (3) 25 μg / mL catmint oil;

[0074] (4) 25 μg / mL verbena oil;

[0075] (5) 25 μg / mL peppermint oil;

[0076] (6) 25 μg / mL benzyl isothiocyanate;

[0077] (7) 25 μg / mL lemongrass oil + 25 μg / mL benzyl isothiocyanate (1:1);

[0078] (8) 25 μg / mL eugenol + 25 μg / mL benzyl isothiocyanate (1:1);

[0079] (9) 25 μg / mL catmint oil + 25 μg / mL benzyl isothiocyanate (1:1);

[0080] (10) 25 μg / mL verbena oil + 25 μg / mL benzyl isothiocyanate (1:1);

[0081] (11) 25 μg / mL peppermint oil + 25 μg / mL benzyl isothiocyanate (1:1);

[0082] Table 2 Indoor contact activity of different plant essential oils and their compound preparations against root-knot nematodes

[0083]

[0084]

[0085] The test adopted the drug immersion method, using a 24-well plate to prepare a certain concentration of drug suspension, and adding the prepared second-instar larvae. After 24 hours and 48 hours, the nematode mortality was observed and counted. The preliminary screening results showed that lemongrass oil, eugenol, nepeta oil, verbena oil, and peppermint oil all had certain contact killing activity against root-knot nematodes at a concentration of 25μg / mL, among which lemongrass oil had the best contact killing activity against root-knot nematodes, with a 48-hour corrected mortality rate of 70.21%. When the concentration of benzyl isothiocyanate was 25μg / mL, the 24-hour and 48-hour control effects on root-knot nematodes were 57.21% and 69.10%, respectively. At the same time, the biological activity of the compound agent was significantly higher than that of the single agent, indicating a synergistic effect.

[0086] Experimental Example 3 Determination of the toxicity of five plant essential oils and benzyl isothiocyanate against root-knot nematodes in indoor fumigation

[0087] The same method as in Experimental Example 1 was used to determine the toxicity of 5 kinds of plant essential oils and benzyl isothiocyanate to root-knot nematodes for indoor fumigation. The results are shown in Table 3:

[0088] Table 3 Analysis of the fumigation activity of five agents against southern root-knot nematodes (48h)

[0089]

[0090] As shown in Table 3, the LC values ​​of five plant essential oils and benzyl isothiocyanate against root-knot nematodes were 50 All of them were at a lower level, indicating that they had higher fumigation activity against root-knot nematodes. The LC 50 The lowest is only 1.25μl / L, followed by benzyl isothiocyanate, which is 2.25μl / L.

[0091] Experimental Example 4: Determination of the combined toxicity of lemongrass oil and benzyl isothiocyanate against root-knot nematodes in the laboratory

[0092] The co-toxicity coefficient (CTC) was calculated by Sun Yunpei method, and the synergistic effect of the compounded drugs was qualitatively evaluated based on the co-toxicity coefficient. First, the LC 50 One of the single agents was set as a standard nematicide and the LC 50The ratio of the single agent and the mixture determines the toxicity index. Finally, the CTC is calculated by the actual toxicity index and theoretical toxicity index of the mixture. The calculation formula is as follows:

[0093]

[0094] Theoretical toxicity index of mixture = ∑ toxicity index of single agent × mass percentage of single agent in mixture

[0095]

[0096] Note: When CTC≥120, it is a synergistic effect; when CTC≤80, it is an antagonistic effect; when 80<CTC<120, it is an additive effect.

[0097] Table 4 Results of the fumigation toxicity test of lemongrass oil and benzyl isothiocyanate compound preparations against root-knot nematodes

[0098]

[0099] Note: In the table, “N” stands for lemongrass oil and “Y” stands for benzyl isothiocyanate.

[0100] The results in Table 4 show that the co-toxicity coefficient CTC of the compound composed of lemongrass oil and benzyl isothiocyanate in the combination of weight ratio of 1:20 to 15:1 is greater than 120, indicating that the two exhibit synergistic effects when mixed in the range of 1:20 to 20:1. When the mass ratio of lemongrass oil to benzyl isothiocyanate is 1:1, the synergistic coefficient is 236.34, and the synergistic effect is most obvious.

[0101] Experimental Example 5: Results of the fumigation toxicity test of eugenol and benzyl isothiocyanate combination against root-knot nematodes

[0102] Table 5 Results of the fumigation toxicity test of eugenol and benzyl isothiocyanate compound preparations against root-knot nematodes

[0103]

[0104] Note: In the table, “D” stands for eugenol and “Y” stands for benzyl isothiocyanate.

[0105] As shown in the results of Table 5, the co-toxicity coefficient CTC of the compound composed of eugenol and benzyl isothiocyanate in the combination of weight ratio of 1:9 to 13:1 is greater than 120, indicating that the two exhibit synergistic effects when mixed in the range of 1:9 to 13:1. When the mass ratio of eugenol to benzyl isothiocyanate is 5:1, the synergistic coefficient is 240.03, and the synergistic effect is most obvious.

[0106] Experimental Example 6: Results of the fumigation toxicity test of nepeta oil and benzyl isothiocyanate combination against root-knot nematodes

[0107] Table 6 Test results of toxicity of nepeta oil and benzyl isothiocyanate compound preparation to root-knot nematode fumigation

[0108]

[0109]

[0110] Note: In the table, "J" stands for catmint oil and "Y" stands for benzyl isothiocyanate.

[0111] As shown in the results of Table 6, the co-toxicity coefficient CTC of the compound composed of catmint oil and benzyl isothiocyanate in the combination of weight ratio of 1:9 to 9:1 is greater than 120, indicating that the two show a synergistic effect when mixed in the range of 1:9 to 9:1. When the mass ratio of catmint oil to benzyl isothiocyanate is 5:1, the synergistic coefficient is 221.57, and the synergistic effect is most obvious.

[0112] Experimental Example 7: Determination of the combined toxicity of verbena oil and benzyl isothiocyanate against Agrobacterium tumefaciens in the laboratory

[0113] The indoor biological activity of the combination of verbena oil and benzyl isothiocyanate against soil-borne pathogenic bacteria Agrobacterium tumefaciens was determined by the inhibition zone method. The synergistic effect of the compounded agents was qualitatively evaluated based on the co-toxicity coefficient using the same method as in Experimental Example 4.

[0114] Table 7 Results of indoor biological activity toxicity test of verbena oil and benzyl isothiocyanate compound preparations against fungi in soil

[0115]

[0116]

[0117] Note: In the table, "M" stands for verbena oil and "Y" stands for benzyl isothiocyanate.

[0118] As shown in the results of Table 7, the co-toxicity coefficient CTC of the compound composed of verbena oil and benzyl isothiocyanate in the combination of a weight ratio of 1:9 to 15:1 is greater than 120, indicating that the two exhibit a synergistic effect when mixed in the range of 1:9 to 15:1. When the mass ratio of verbena oil to benzyl isothiocyanate is 3:1, the synergistic coefficient is 151.26, and the synergistic effect is most obvious.

[0119] Experimental Example 8: Determination of the combined toxicity of peppermint oil and benzyl isothiocyanate against watermelon wilt pathogen in the laboratory

[0120] The inhibition zone method was used to determine the indoor biological activity of the combination of verbena oil and benzyl isothiocyanate against the soil-borne pathogenic fungus Fusarium wilt of watermelon, and the synergistic effect of the combined agents was qualitatively evaluated based on the co-toxicity coefficient.

[0121] Table 8 Results of indoor bioactivity toxicity test of peppermint oil and benzyl isothiocyanate compound preparations against bacteria in soil

[0122]

[0123] Note: In the table, "B" stands for peppermint oil and "Y" stands for benzyl isothiocyanate.

[0124] As shown in the results of Table 8, the co-toxicity coefficient CTC of the compound composed of peppermint oil and benzyl isothiocyanate in the combination of a weight ratio of 1:15 to 20:1 is greater than 120, indicating that the two exhibit a synergistic effect when mixed in the range of 1:15 to 20:1. When the mass ratio of peppermint oil to benzyl isothiocyanate is 1:5, the synergistic coefficient is 145.10, and the synergistic effect is most obvious.

[0125] Experimental Example 9: Potted plant test on five kinds of plant essential oils, benzyl isothiocyanate and compound preparations against root-knot nematodes

[0126] The soil of the tillage layer (depth of about 0-15cm) of the tomato field where the root-knot nematode disease is seriously infested in the vegetable planting base of Dali County, Shaanxi Province is kept warm and moisturized, and the diseased roots of tomatoes infected with the root-knot nematode disease are buried and allowed to rot in the soil. After a period of time, the soil contains a large number of tomato root-knot nematode larvae. Then the above soil (absolute water content of about 20%) is mixed evenly and put into flower pots, 1kg per pot. Then, according to the following concentration gradient: 50, 200μL / kg (weight ratio of active ingredient to soil), the compound preparation is diluted and stirred with water, and then mixed with soil. Finally, the pots and sands that have been medicated are sealed with polyvinyl chloride plastic film for 7d, and after opening for 4d, the tomato seedlings that have been cultivated for 14d are transplanted. At the same time, a 20% horseradish water emulsion with a concentration of 50mg / kg is set as the drug control and a treatment without any drug is set as the blank control. Each treatment is repeated 4 times and cultivated under the same water and fertilizer management conditions. The statistical results after 45 days are as follows.

[0127] Table 9 Control effect of each preparation on root-knot nematode pot test

[0128]

[0129]

[0130] From the results of the potted test, it can be seen that the protective effects of lemongrass oil, eugenol, nepeta oil, verbena oil, peppermint oil and benzyl isothiocyanate under the treatment of 200 μl / kg were 78.32%, 67.56%, 64.56%, 60.33%, 58.09% and 70.11%, respectively, all of which have high control effects on root-knot nematodes. Under the 200 μl / kg treatment, the protective effect of lemongrass oil: benzyl isothiocyanate (1:1) was 100%, eugenol: benzyl isothiocyanate (5:1) was 89.55%, nepeta oil: benzyl isothiocyanate (5:1) was 80.33%, verbena oil: benzyl isothiocyanate (3:1) was 79.34%, and peppermint oil: benzyl isothiocyanate (1:5) was 71.99%, all of which were higher than the protective effect of benzyl isothiocyanate alone against root-knot nematodes of 70.11%, indicating that plant essential oils and benzyl isothiocyanate have a significant synergistic effect when used in combination, and can also reduce the use of a single agent and delay resistance.

[0131] Experimental Example 10 Field test of five kinds of plant essential oils, benzyl isothiocyanate and compound preparations against root-knot nematodes

[0132] The field test was conducted in a root-knot nematode-prone area in Dali County, Shaanxi Province (with an incidence index of more than 5 levels). The plot was planted with a single crop for many years, and the root-knot nematode population was difficult to control. The previous crop planted on the plot was watermelon, and the plot had not been treated with pesticides one year before the test. The plot was planted in single ridges and double rows. Normal land preparation and management were carried out before the test, and no nematicides were applied. Before transplanting the test pumpkin seedlings, the pesticide was diluted with water and the soil was irrigated until the 25cm soil layer under the soil was soaked. After treatment, the ridges were sealed with polyethylene plastic film, and clean water was used as a blank control. Clean water and film were applied in the same way. After sealing for 7 days, it was opened for 4 days and the pumpkin seedlings were transplanted. The control agent was 20% horseradish emulsion in water, and the treatment method was the same. The root knot index of each treatment was investigated 60 days after the application of the pesticide, and the control effect of each agent was calculated.

[0133] Table 10 Control effect of each preparation on root-knot nematode field test

[0134]

[0135] Field trials have shown that at 12g / m 2Under the following dosage, the field test control effect of lemongrass oil: benzyl isothiocyanate (1:1) on root-knot nematodes is 80.43%, eugenol: benzyl isothiocyanate (5:1) is 75.99%, nepeta oil: benzyl isothiocyanate (5:1) is 70.82%, verbena oil: benzyl isothiocyanate (3:1) is 71.73%, peppermint oil: benzyl isothiocyanate (1:5) is 72.11%, all of which are higher than the use of essential oils and isothiocyanates alone. It is said that the plant essential oil and benzyl isothiocyanate plant-based composition of the invention has a significant synergistic effect.

[0136] Example 1: Production of Brassica oleracea A using a combination of 14% lemongrass oil and 6% benzyl isothiocyanate

[0137] Accurately weigh 7 kg of lemongrass oil and 3 kg of benzyl isothiocyanate. 3 Mix thoroughly in the mixing tank. Accurately weigh 0.8 kg of ascorbic acid, put it into the mixing tank (1) and mix thoroughly again. Then accurately add 25 kg of ethyl formate and 14.2 kg of soybean oil into the mixing tank and mix thoroughly in the mixing tank. Continue stirring for 1 hour. Prepare an oil product of 50 kg of lemongrass oil and benzyl isothiocyanate composition. Sampling analysis showed that the content of lemongrass oil was 19.88% and the content of benzyl isothiocyanate was 9.98%. The product was divided into aluminum cans coated with barrier material, 5 kg per can, for a total of 10 cans. Seal the can mouth and label it to obtain the fungicide and nematicidal oil product A containing 29.86% lemongrass oil and benzyl isothiocyanate composition.

[0138] Example 2: Production of a soluble liquid formulation of 30% verbena essential oil and 10% benzyl isothiocyanate composition of scutellaria baicalensis B

[0139] Accurately weigh 180 kg of verbena essential oil and 60 kg of benzyl isothiocyanate original drug. Thoroughly mix the two active drug liquids in the preparation tank (1). Accurately weigh 30 kg of ascorbic acid and put it into the preparation tank (1) and mix it again. Add 40 kg of emulsifier 500# and 50 kg of emulsifier OP-10 to the preparation tank (2), mix them, and then add 240 kg of ethyl formate and mix them thoroughly. Transfer all the kilograms of material in the preparation tank (2) into the preparation tank (1) through the pipeline, seal the tank mouth, and start the mixer to stir for 1 hour. Prepare 600 kg of a soluble liquid product of verbena essential oil and benzyl isothiocyanate composition. Sampling analysis of the product showed that the content of verbena essential oil was 29.88%, and benzyl isothiocyanate was 9.98%, which resulted in a 39.86% mycelium-killing brassicain B product. The product was packaged into double-barrier plastic bottles, with 500 mL in each bottle.

[0140] Example 3: Production of 20% eugenol and 8% benzyl isothiocyanate emulsion for the production of clematisin C

[0141] Accurately weigh 64 kg of benzyl isothiocyanate original drug, 160 kg of eugenol original drug, and 16 kg of ascorbic acid, and stir the three in a mixing tank. Then quantitatively add 120 kg of agricultural emulsifier 1601, 250 kg of ethyl formate, and 190 kg of water to the mixing tank, stirring while adding. After all the materials are added, continue to stir with a stirrer for 1 hour. The amount of benzyl isothiocyanate contained in the sampled product is 7.99%, and the content of eugenol is 19.79%. Finally, 800 kg of products with a total content of effective components of 27.78% are obtained, and the product is bottled with a 500 mL glass bottle and the mouth is sealed as in Example 1.

[0142] Example 4: Production of soluble liquid bacteria-killing brassica cyanin D from a composition of 35% catmint oil and 12% benzyl isothiocyanate

[0143] Accurately weigh 120 kg of benzyl isothiocyanate technical and 350 kg of catmint oil. Mix the two active liquids in a mixing tank (1). Accurately weigh 15 kg of ascorbic acid and put it into the mixing tank (1) and mix it again. Add 60 kg of agricultural milk 500 and 80 kg of emulsifier 1602 into the mixing tank (2), mix them, and then add 375 kg of ethyl formate and mix them thoroughly. Transfer all 515 kg of materials in the mixing tank (2) into the mixing tank (1) through a pipeline, seal the tank mouth, start the mixer and stir for 1 hour. Prepare 1 ton of catmint oil·benzyl isothiocyanate soluble liquid product. Sampling analysis shows that the content of benzyl isothiocyanate in the product is 12%, and the content of catmint oil is 35%, that is, it becomes a 47% fungal line killing brassica cyanin D product, and the product is divided into double-barrier plastic bottles, each bottle containing 500 mL of medicine.

[0144] Example 5: Production of Brassica oleracea E using a microemulsion of 40% peppermint oil and 15% benzyl isothiocyanate

[0145] Accurately weigh 300 kg of benzyl isothiocyanate and 800 kg of peppermint oil. Mix the two active liquids in a mixing tank (1). Accurately weigh 40 kg of ascorbic acid and put it into the mixing tank (1) and mix it again. Add 40 kg of Nongru 602 and 100 kg of emulsifier OP-10 to the mixing tank (2), mix them, and then add 720 kg of ethyl formate and mix them thoroughly. Transfer all the kilograms of materials in the mixing tank (2) into the mixing tank (1) through a pipeline, seal the tank mouth, and start the mixer to stir for 1 hour. Prepare 2000 kg of peppermint oil·benzyl isothiocyanate soluble liquid product. Sampling analysis shows that the content of peppermint oil in the product is 40%, and the content of benzyl isothiocyanate is 14.99%, that is, it becomes a 54.99% fungal line killing brassicain E product. The product is divided into double-barrier plastic bottles, each bottle containing 500 mL of medicine.

[0146] Example 6: Production of 3% lemongrass oil and 3% benzyl isothiocyanate granules to kill cauliflower F

[0147] Accurately weigh 6 kg of benzyl isothiocyanate and 6 kg of lemongrass oil. Mix the two active liquids thoroughly in the mixing tank (1). Accurately weigh 144 kg of starch and put it into the mixing tank (1) and mix it thoroughly again. Add 6 kg of sodium carboxymethyl cellulose as an adhesive, 19 kg of bentonite as a disintegrant, and 19 kg of calcium lignin sulfonate as an auxiliary agent into the mixing tank (2) and mix them thoroughly. Transfer all 22 kg of materials in the mixing tank (2) into the mixing tank (1) through the pipeline, seal the tank mouth and start the mixer to stir for 1 hour. During this period, slowly add water several times. When the materials are in a state of being able to be grasped into a ball and loosened by light squeezing, put all the materials in the mixing tank (1) into the extrusion granulator in batches for granulation, put the prepared granules into a drying oven at 60°C for 4 hours, and prepare 200 kg of lemongrass oil·benzyl isothiocyanate granules. The content of benzyl isothiocyanate in the granules sampled and tested was 2.98%, the content of lemongrass oil was 2.92%, and the total content of effective components in the product was 5.9%. The granules of qualified quality were packaged with suitable packaging materials, such as aluminum foil bags or plastic bottles.

[0148] Embodiment 7:

[0149] In the Weinan vegetable base in Shaanxi Province, a field where cucumbers are planted all year round and has been seriously damaged by cucumber root-knot nematodes for a long time was selected. The fields were divided into small plots of the same size, each with an area of ​​30m2. The plots were randomly arranged and holes of the same size were opened. The roots were irrigated in each hole. The control agent was 20% horseradish emulsion in water, and the treatment group agents were 8% benzyl isothiocyanate emulsifiable concentrate (8% benzyl isothiocyanate + 10% emulsifier 1601 + 82% ethyl acetate) and 25% bacterial line-killing brassica napus A oil. They were diluted 2000 times and 250ml was applied in the holes. The statistical results after two months showed that the control group, 8% benzyl isothiocyanate emulsifiable concentrate, and 25% bacterial line-killing brassica napus A oil had a protection rate of 65.87%, 89.45%, and 70.11%, respectively.

[0150] Embodiment 8:

[0151] At the Luoyang Vegetable Experimental Base in Henan Province, a field with severe root-knot nematode infection on water spinach was selected and divided into small plots of the same size, which were randomly arranged. Each plot had an area of ​​50 m 2 , direct pouring, film covering, soil fumigation treatment for 7 days, transplant seedlings at intervals of 14 days. The control agent is 20% thiazolyl emulsion (Jiangsu Huifeng Bio-Agriculture Co., Ltd.), the treatment group agent is 10% benzyl isothiocyanate emulsifiable concentrate (10% benzyl isothiocyanate + 8% Tween-80 + 5% agricultural milk 500 + 50% sec-butyl acetate + 27% ethyl acetate), 40% bacterial line killing cabbage blue Y soluble liquid, diluted 1200 times, each treatment per square meter of 15g, two months later, the number of root knots per gram of root was counted, and the prevention effect was 49.78%, 70.32%, and 92.67% respectively.

[0152] Embodiment 9:

[0153] At the Gaoming Vegetable Base in Foshan, a field with severe clubroot infection was selected and divided into three plots, each with an area of ​​100m 2 . The method of trenching was adopted, and a trench was opened every 0.6m. Three treatment methods were set up: 27.78% aqueous emulsion bacterial line killing cabbage blue C produced in Example 3, 12% benzyl isothiocyanate oil (12% benzyl isothiocyanate + 6% OP-10 + 6% agricultural milk 500 # + 40% ethyl acetate + 42% ethyl formate), and clean water. All were diluted 1200 times and irrigated into the trench. The dosage for each trench was 10L, covered with soil, and then covered with a thick film. It was sealed for seven days, then opened to air for four days, and cabbage was planted. The management measures for each treatment after sowing were the same. When the cabbage harvest season came, the root swelling was checked. The clean water control was 96.89%, the root swelling infection rate of the 27.78% aqueous emulsion bacterial line killing cabbage blue C treatment group was 6.89%, and the root swelling infection rate of the 12% benzyl isothiocyanate oil treatment group was 15.32%.

[0154] Embodiment 10:

[0155] Use soil with serious watermelon wilt to fill the bowls, and use 6 kg of soil for each bowl. A total of 60 bowls are filled, of which 30 bowls are filled with 250 mL of clean water as blank controls. First, use a double-layer nylon film bag to wrap the blank control bowls tightly. Then take the 47% soluble liquid bacteriomycin D produced in Example 4, dilute it 1200 times, and fill each bowl with 250 mL of the diluted solution, and cover it with soil. As soon as possible, use a nylon bag to wrap each bowl tightly according to the method of wrapping the blank control, seal it for 7 days, remove the wrapped film, and open it for 7 days. Then transplant the watermelon seedlings, two plants per bowl. Let the watermelon seedlings grow for 45 days under the same management conditions. 50 of the 60 watermelon seedlings in the blank control died, and 6 of the 60 watermelon seedlings on the soil treated with the agent of the present invention died, and the prevention effect reached 90%.

[0156] Embodiment 11:

[0157] The soil blocks seriously infected with cruciferous soft rot were mixed evenly and put into flower pots, 1 kg per pot. Then 1.26 g of 7.9% granular bacteria line killing brassica quinata F produced in Example 6 was weighed and evenly mixed into the soil, and then a small amount of clean water was poured in. The pots and sands with the drug were sealed with polyvinyl chloride plastic film for 7 days, and after opening for 4 days, rapeseed seeds were sown in the flower pots, with 200 seeds in both the blank and treatment groups. The statistical results after half a month showed that the seedling rate was statistically analyzed, and the control group had almost no survival. The mortality rate of the drug treatment group was only 15.78%.

[0158] Although the present invention has been described in detail above with general descriptions and specific embodiments, it is obvious to those skilled in the art that some modifications or improvements may be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection claimed by the present invention.

Claims

1. A composition containing plant essential oil and benzyl isothiocyanate, characterized in that: The effective components of the composition are active ingredient A and active ingredient B; The active ingredient A is benzyl isothiocyanate; The active ingredient B is at least one selected from lemongrass oil, eugenol, nepeta oil, verbena oil and peppermint oil; The weight ratio of active ingredient A to active ingredient B is (1-20):(20-1).

2. The composition containing plant essential oil and benzyl isothiocyanate according to claim 1, characterized in that: The composition comprises: The weight ratio of benzyl isothiocyanate to lemongrass oil is 1:20 to 15:1; The weight ratio of benzyl isothiocyanate to eugenol is 13:1 to 1:9; The weight ratio of benzyl isothiocyanate to catmint oil is 9:1 to 1:9; The weight ratio of benzyl isothiocyanate to verbena oil is 15:1 to 1:9; The weight ratio of benzyl isothiocyanate to peppermint oil is 20:1 to 1:

15.

3. A composition preparation containing plant essential oil and benzyl isothiocyanate, characterized in that: The preparation contains the composition containing plant essential oil and benzyl isothiocyanate according to any one of claims 1-2; Calculated by mass percentage, in the preparation, the active ingredient A is 1% to 15%, and the active ingredient B is 1% to 30%.

4. The composition preparation containing plant essential oil and benzyl isothiocyanate according to claim 3, characterized in that: The preparation is an oil, and the components and weight percentages are: Active ingredient A 1% to 15%, active ingredient B 1% to 30%, antioxidant 0.1% to 2%, solvent 10% to 85%, vegetable oil to make up to 100%; Wherein, the antioxidant is ascorbic acid or amyl gallate; the solvent is at least one of ethyl formate, 2-butyl acetate, furfural, 5-hydroxymethylfurfural, 5-methylfurfural or ethyl acetate; and the vegetable oil is turpentine or Sichuan dove oil.

5. The composition preparation containing plant essential oil and benzyl isothiocyanate according to claim 3, characterized in that: The preparation is an aqueous emulsion, and the components and weight percentages are: Active ingredient A 1% to 10%, active ingredient B 1% to 35%, emulsifier 12% to 28%, ascorbic acid 0.1% to 2%, solvent 10% to 30%, and water to make up to 100%; Wherein, the emulsifier is Nongru 500, 1601, Tween-80, TX-10, OP-10 or Span 80; the solvent is at least one of ethyl formate, 2-butyl acetate, furfural, 5-hydroxymethylfurfural, 5-methylfurfural or ethyl acetate.

6. The composition preparation containing plant essential oil and benzyl isothiocyanate according to claim 3, characterized in that: The preparation is a soluble liquid, and the components and weight percentages are: Active ingredient A 1% to 20%, active ingredient B 1% to 30%, emulsifier 12% to 60%, ascorbic acid 1% to 8%, and solvent to 100%; Wherein, the emulsifier is Nongru 500, 1601, Tween-80, TX-10, OP-10 or Span 80; the solvent is at least one of ethyl formate, sec-butyl acetate, furfural, 5-hydroxymethylfurfural, 5-methylfurfural or ethyl acetate.

7. The composition preparation containing plant essential oil and benzyl isothiocyanate according to claim 3, characterized in that: The preparation is a microemulsion, and the components and weight percentages are: Active ingredient A 1% to 15%, active ingredient B 1% to 30%, solvent 10% to 40%, emulsifier 5% to 20%, ascorbic acid 0.1% to 2%; Wherein, the emulsifier is Nongru 500, 1601, Tween-80, TX-10, OP-10 or Span 80; the solvent is at least one of ethyl formate, 2-butyl acetate, furfural, 5-hydroxymethylfurfural, 5-methylfurfural or ethyl acetate.

8. The composition preparation containing plant essential oil and benzyl isothiocyanate according to claim 3, characterized in that: The preparation is a granule, and the components and weight percentages are: Active ingredient A 1% to 10%, active ingredient B 1% to 30%, carrier filler 60% to 80%, disintegrant 1% to 3%, binder 1% to 3%, auxiliary agent 0% to 10%; The carrier filler is selected from at least one of starch, kaolin, diatomaceous earth and light calcium carbonate; the disintegrant package is selected from at least one of cellulose ether and its derivatives, sodium carboxymethyl starch and bentonite; the binder is selected from at least one of sodium carboxymethyl cellulose, xanthan gum and polyethylene glycol; the auxiliary agent is selected from at least one of glucose, calcium lignin sulfonate and magnesium aluminum silicate.

9. Use of the composition containing plant essential oil and benzyl isothiocyanate according to any one of claims 1-2 or the composition preparation containing plant essential oil and benzyl isothiocyanate according to any one of claims 3-8 for preparing a fumigant for controlling soil-borne pathogens and / or soil nematodes.

10. The use according to claim 9, characterized in that: The soil-borne pathogens include Agrobacterium tumefaciens and / or Fusarium wilt; The soil nematodes are root-knot nematodes.

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