Composition with antioxidant function as well as preparation method and application thereof

Abstract

The invention relates to the technical field of food and / or medicine, and provides a composition with an anti-oxidation function and a preparation method and application thereof, and the composition comprises the following raw materials by weight: 0.5-10 parts of Radix Astragali; 0.5 to 10 parts of radix acanthopanacis senticosi; 0.5 to 10 parts of red sage root; 0.5 to 10 parts of fiveleaf gynostemma herb; 0.05 to 1 part of haematococcus pluvialis oil powder; and 0.1 to 0.9 part of grape seed extract. The composition provided by the invention has an anti-oxidation effect, is helpful for improving the anti-oxidation capability of a human body, slowing down aging and preventing chronic diseases, and has a good market development prospect.

Description

Technical Field

[0001] The present invention relates to the technical field of food and / or medicine, and in particular to a composition with antioxidant function and a preparation method and application thereof. Background Art

[0002] With daily activities, the human body continuously produces free radicals. Free radicals are products of the normal metabolic process of animal organisms and are highly oxidizing. Under normal conditions, the body's oxidation and antioxidant systems maintain a dynamic balance. Once the balance is broken, it will cause damage to the body and even trigger a series of diseases. Free radicals and the oxidative reactions they induce are important factors leading to biological aging and nerve damage. They are also related to the occurrence of many diseases such as inflammation, cancer, osteoarthritis, rheumatoid arthritis, diabetes, premature aging, cardiovascular disease, and cognitive dysfunction caused by oxidative damage to brain tissue. To reduce the harm of free radicals to the human body, in addition to relying on the free radical scavenging system in the body, it is also necessary to find and explore exogenous free radical scavengers, use scavengers to combine with free radicals to block external free radical attacks and protect the human body from harm.

[0003] At present, no effective drugs or specific means have been found to treat cognitive dysfunction caused by oxidative damage. For antioxidant treatment, traditional antioxidants such as vitamin C and β-carotene have poor stability, unsatisfactory antioxidant effects, and are easily inactivated. In many cases, the expected therapeutic effect is not achieved, and long-term and large-scale use will cause toxic side effects such as fatigue, vomiting, and diarrhea. Natural antioxidant active substances can effectively remove free radicals from the human body and delay aging. There are abundant natural antioxidant ingredients in plant foods and Chinese medicinal materials. Traditional Chinese medicine has shown outstanding advantages in the treatment of many complex diseases, such as fewer side effects, long-lasting efficacy, high patient compliance, and the ability to act on multiple targets, which significantly improves the clinical symptoms and prognosis of patients.

[0004] Therefore, searching for natural antioxidants has become a research hotspot. Finding safe, efficient, cheap, and low-toxic natural antioxidants from nature is the current development trend of the antioxidant industry in the field of traditional Chinese medicine and food. It is of great significance to the utilization and development of new functional antioxidants, and provides new clues and hopes for human health and delaying aging. Summary of the invention

[0005] The purpose of the present invention is to provide a composition with antioxidant function and a preparation method and application thereof.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] A composition with antioxidant function comprises the following raw materials in parts by weight: 0.5-10 parts of astragalus, 0.5-10 parts of acanthopanax, 0.5-10 parts of salvia miltiorrhiza, 0.5-10 parts of gynostemma pentaphyllum, 0.05-1 parts of Haematococcus pluvialis oil powder and 0.1-0.9 parts of grape seed extract.

[0008] Furthermore, the composition with antioxidant function comprises the following raw materials in parts by weight: 1-5 parts of astragalus; 1-5 parts of acanthopanax senticosus; 1-5 parts of salvia miltiorrhiza; 1-5 parts of gynostemma pentaphyllum; 0.1-0.5 parts of Haematococcus pluvialis oil powder and 0.2-0.7 parts of grape seed extract.

[0009] Furthermore, the composition with antioxidant function comprises the following raw materials in parts by weight: 10 parts of Astragalus, 0.5 parts of Acanthopanax, 10 parts of Salvia miltiorrhiza, 0.5 parts of Gynostemma pentaphyllum, 1 part of Haematococcus pluvialis oil powder and 0.1 parts of grape seed extract.

[0010] Furthermore, the composition with antioxidant function comprises the following raw materials in parts by weight: 3 parts of Astragalus, 3 parts of Acanthopanax, 3 parts of Salvia miltiorrhiza, 3 parts of Gynostemma pentaphyllum, 0.36 parts of Haematococcus pluvialis oil powder and 0.5 parts of grape seed extract.

[0011] Furthermore, the composition with antioxidant function comprises the following raw materials in parts by weight: 5 parts of Astragalus; 1 part of Acanthopanax; 5 parts of Salvia miltiorrhiza; 1 part of Gynostemma pentaphyllum; 0.1 part of Haematococcus pluvialis oil powder and 0.7 part of grape seed extract.

[0012] Furthermore, the preparation method of the above composition comprises the following steps:

[0013] (1) Take Astragalus, Acanthopanax, Salvia miltiorrhiza, and Gynostemma pentaphyllum, add water and reflux to extract twice, filter, and obtain an extract;

[0014] (2) concentrating the extract under reduced pressure to a relative density of 1.05 to 1.15 (60° C.) to obtain an extract, drying the extract to obtain a dry paste, and crushing the dry paste to obtain a dry paste powder;

[0015] (3) The obtained dry paste powder, grape seed extract and Haematococcus pluvialis oil powder together constitute the composition.

[0016] Furthermore, in step (1), the extraction is performed twice for 1.0 to 2.0 hours each time, and 9 to 11 times of water is added each time; in step (2), the temperature for reduced pressure concentration is 50 to 80° C., and the vacuum degree is 0.03 to 0.09 MPa; in step (2), the drying temperature is 110 to 120° C., and the vacuum degree is 0.04 to 0.10 MPa, and the moisture content of the obtained dry paste is ≤8%.

[0017] The present invention also provides the use of the composition with antioxidant function in the preparation of health food, functional food or medicine.

[0018] In addition, the present invention also provides a health food, comprising the above-mentioned composition with antioxidant function.

[0019] Furthermore, the health food also includes the following raw materials and auxiliary materials in parts by weight: 1-2 parts of microcrystalline cellulose; 0.1-1 parts of cross-linked polyvinylpyrrolidone; 0.05-0.5 parts of polyvinylpyrrolidone; 0.05-0.1 parts of silicon dioxide and 0.01-0.08 parts of magnesium stearate.

[0020] Furthermore, the dosage form of the health food is granules, tablets or capsules.

[0021] Furthermore, the preparation method of the health food tablets comprises the following steps:

[0022] S1. Take Astragalus, Acanthopanax, Salvia miltiorrhiza, and Gynostemma pentaphyllum, add water and reflux to extract twice, filter, and obtain an extract;

[0023] S2, the extract is concentrated under reduced pressure to a relative density of 1.05-1.15 (60°C) to obtain an extract, the extract is dried to obtain a dry paste, and the dry paste is crushed to obtain a dry paste powder;

[0024] S3, mixing the dry paste powder, microcrystalline cellulose and grape seed extract, adding an ethanol solution of povidone to prepare a soft material, granulating, and drying to obtain dry granules;

[0025] S4, premixing the Haematococcus pluvialis oil powder and cross-linked polyvinylpyrrolidone to obtain a premixed powder, and mixing the premixed powder with the obtained dry granules, silicon dioxide, and magnesium stearate to obtain a total mixed granule;

[0026] S5. Compressing and coating the obtained total mixed granules to obtain the product.

[0027] Furthermore, in step S1, each extraction is for 1.0 to 2.0 hours, and 9 to 11 times of water is added each time; in step S2, the temperature of reduced pressure concentration is 50 to 80°C, and the vacuum degree is 0.03 to 0.09 MPa; in step S2, the drying temperature is 110 to 120°C, the vacuum degree is 0.04 to 0.10 MPa, and the moisture content of the obtained dry paste is ≤8%; in step S3, the drying temperature is 50 to 60°C, and the moisture content of the particles is controlled to be ≤8%.

[0028] Compared with the prior art, the composition with antioxidant function provided by the present invention is composed of dry powder of water extract of Astragalus, Acanthopanax, Salvia miltiorrhiza, Gynostemma pentaphyllum and grape seed extract and Haematococcus pluvialis oil powder in a specific ratio, which can reduce the level of hemolytic MDA, increase serum SOD activity, and increase whole blood GSH content. The components have a synergistic antioxidant effect after combination, and the obtained composition has good antioxidant function. The composition provided by the present invention can be used as an active ingredient in the preparation of food, health food or medicine with antioxidant effect, which meets people's new demand for health, helps to improve the antioxidant capacity of the human body, slow down aging and prevent chronic diseases, and has good market development prospects. DETAILED DESCRIPTION

[0029] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0030] Example 1

[0031] A composition with antioxidant function is prepared from the following raw materials in parts by weight: 3 parts of astragalus, 3 parts of acanthopanax, 3 parts of salvia miltiorrhiza, 3 parts of gynostemma pentaphyllum, 0.36 parts of Haematococcus pluvialis oil powder and 0.5 parts of grape seed extract.

[0032] The preparation method of the composition with antioxidant function is as follows:

[0033] (1) Weigh astragalus, Acanthopanax senticosus, Salvia miltiorrhiza, and Gynostemma pentaphyllum according to the formula ratio, add water and reflux to extract twice, each time for 1.5 hours, add 10 times of water each time, filter, and combine the filtrates to obtain an extract;

[0034] (2) the extract is concentrated under reduced pressure (concentration temperature is 60° C., vacuum degree is 0.06 MPa) to a relative density of 1.05 to 1.15 (60° C.) to obtain an extract, the extract is dried at a drying temperature of 115° C. and a vacuum degree of 0.06 MPa to obtain a dry paste (water content ≤ 8%), the dry paste is crushed (sieve mesh number: 80 mesh), and the dry paste powder is collected;

[0035] (3) The obtained dry powder is mixed with grape seed extract and Haematococcus pluvialis oil powder to form a composition with antioxidant function.

[0036] Example 2

[0037] A composition with antioxidant function comprises the following raw materials in parts by weight: 10 parts of astragalus, 0.5 parts of acanthopanax, 10 parts of salvia miltiorrhiza, 0.5 parts of gynostemma pentaphyllum, 1 part of haematococcus pluvialis oil powder and 0.1 parts of grape seed extract.

[0038] The preparation method of the composition with antioxidant function is as follows:

[0039] (1) Weigh astragalus, Acanthopanax senticosus, Salvia miltiorrhiza, and Gynostemma pentaphyllum according to the formula ratio, add water and reflux to extract twice, each time for 1.0 hour, add 11 times of water each time, filter, and combine the filtrates to obtain an extract;

[0040] (2) the extract is concentrated under reduced pressure (concentration temperature is 80° C., vacuum degree is 0.03 MPa) to a relative density of 1.05 to 1.15 (60° C.) to obtain an extract, the extract is dried at a drying temperature of 120° C. and a vacuum degree of 0.04 MPa to obtain a dry paste (water content ≤ 8%), the dry paste is crushed (sieve mesh number: 80 mesh), and the dry paste powder is collected;

[0041] (3) The obtained dry powder is mixed with grape seed extract and Haematococcus pluvialis oil powder to form a composition with antioxidant function.

[0042] Example 3

[0043] A composition with antioxidant function comprises the following raw materials in parts by weight: 5 parts of astragalus, 1 part of acanthopanax senticosus, 5 parts of salvia miltiorrhiza, 1 part of gynostemma pentaphyllum, 0.1 part of Haematococcus pluvialis oil powder and 0.7 part of grape seed extract.

[0044] The preparation method of the composition with antioxidant function is as follows:

[0045] (1) Weigh astragalus, Acanthopanax senticosus, Salvia miltiorrhiza, and Gynostemma pentaphyllum according to the formula ratio, add water and reflux to extract twice, each time for 2.0 hours, add 9 times water each time, filter, and combine the filtrates to obtain an extract;

[0046] (2) the extract is concentrated under reduced pressure (concentration temperature is 50° C., vacuum degree is 0.09 MPa) to a relative density of 1.05 to 1.15 (60° C.) to obtain an extract, the extract is dried at a drying temperature of 110° C. and a vacuum degree of 0.04 MPa to obtain a dry paste (water content ≤ 8%), the dry paste is crushed (sieve mesh number: 80 mesh), and the dry paste powder is collected;

[0047] (3) The obtained dry powder is mixed with grape seed extract and Haematococcus pluvialis oil powder to form a composition with antioxidant function.

[0048] Example 4

[0049] A composition with antioxidant function comprises the following raw materials in parts by weight: 0.5 parts of astragalus, 10 parts of acanthopanax, 0.5 parts of salvia miltiorrhiza, 10 parts of gynostemma pentaphyllum, 0.05 parts of Haematococcus pluvialis oil powder and 0.9 parts of grape seed extract.

[0050] The preparation method of the composition with antioxidant function is as follows:

[0051] (1) Weigh astragalus, Acanthopanax senticosus, Salvia miltiorrhiza, and Gynostemma pentaphyllum according to the formula ratio, add water and reflux to extract twice, each time for 1.5 hours, add 10 times of water each time, filter, and combine the filtrates to obtain an extract;

[0052] (2) the extract is concentrated under reduced pressure (concentration temperature is 70° C., vacuum degree is 0.07 MPa) to a relative density of 1.05 to 1.15 (60° C.) to obtain an extract, the extract is dried at a drying temperature of 115° C. and a vacuum degree of 0.07 MPa to obtain a dry paste (water content ≤ 8%), the dry paste is crushed (sieve mesh number: 80 mesh), and the dry paste powder is collected;

[0053] (3) The obtained dry powder is mixed with grape seed extract and Haematococcus pluvialis oil powder to form a composition with antioxidant function.

[0054] Example 5

[0055] A composition with antioxidant function comprises the following raw materials in parts by weight: 1 part of astragalus, 5 parts of acanthopanax senticosus, 1 part of salvia miltiorrhiza, 5 parts of gynostemma pentaphyllum, 0.5 part of Haematococcus pluvialis oil powder and 0.2 part of grape seed extract.

[0056] The preparation method of the composition with antioxidant function is as follows:

[0057] (1) Weigh astragalus, Acanthopanax senticosus, Salvia miltiorrhiza, and Gynostemma pentaphyllum according to the formula ratio, add water and reflux to extract twice, the first extraction time is 1.2 hours, the first time water is added 11 times, the second extraction time is 1.8 hours, the second time water is added 9 times, filter, and combine the filtrates to obtain an extract;

[0058] (2) the extract is concentrated under reduced pressure (concentration temperature is 65° C., vacuum degree is 0.08 MPa) to a relative density of 1.05 to 1.15 (60° C.) to obtain an extract, the extract is dried at a drying temperature of 110° C. and a vacuum degree of 0.08 MPa to obtain a dry paste (water content ≤ 8%), the dry paste is crushed (sieve mesh number: 80 mesh), and the dry paste powder is collected;

[0059] (3) The obtained dry powder is mixed with grape seed extract and Haematococcus pluvialis oil powder to form a composition with antioxidant function.

[0060] Example 6

[0061] A health food, comprising the composition with antioxidant function obtained in Example 1. The raw materials and auxiliary materials are as follows:

[0062] 3 parts of Astragalus; 3 parts of Acanthopanax senticosus; 3 parts of Salvia miltiorrhiza; 3 parts of Gynostemma pentaphyllum; 0.36 parts of Haematococcus pluvialis oil powder; 0.5 parts of grape seed extract: 1.428 parts of microcrystalline cellulose (PH101); 0.664 parts of cross-linked polyvinylpyrrolidone; 0.112 parts of polyvinylpyrrolidone (K30); 0.08 parts of silicon dioxide; 0.056 parts of magnesium stearate and 0.176 parts of film coating premix.

[0063] The preparation method of the above health food is as follows:

[0064] S1. Weigh astragalus, Acanthopanax, Salvia miltiorrhiza, and Gynostemma pentaphyllum according to the formula ratio, add water and reflux to extract twice, each time for 1.5 hours, add 10 times water each time, filter, and combine the filtrates to obtain an extract;

[0065] S2, the extract is concentrated under reduced pressure (concentration temperature is 60°C, vacuum degree is 0.06MPa) to a relative density of 1.05-1.15 (60°C) to obtain an extract, the extract is dried at a drying temperature of 115°C and a vacuum degree of 0.06MPa to obtain a dry paste (water content ≤ 8%), the dry paste is crushed (sieve mesh number: 80 mesh), and the dry paste powder is collected;

[0066] S3, mixing the dry paste powder, microcrystalline cellulose and grape seed extract, using 6% povidone K30 in 90% ethanol solution as a binder to prepare a soft material, granulating, drying the wet granules at a drying temperature of 50-60° C., controlling the granule moisture content within 8%, and granulating to obtain dry granules;

[0067] S4, taking the Haematococcus pluvialis oil powder and cross-linked polyvinylpyrrolidone for premixing (rotation speed is 8 r / min, premixing for 10 min) to obtain a premixed powder, and mixing the premixed powder with the obtained dry granules, silicon dioxide, and magnesium stearate (rotation speed is 8 r / min, mixing for 30 min) to obtain a total mixed granule;

[0068] S5. Compressing and coating the obtained total mixed granules to obtain the product.

[0069] Example 7

[0070] A health food, comprising the composition with antioxidant function obtained in Example 2. The raw materials and auxiliary materials are as follows:

[0071] 10 parts of Astragalus, 0.5 parts of Acanthopanax, 10 parts of Salvia miltiorrhiza, 0.5 parts of Gynostemma pentaphyllum, 1 part of Haematococcus pluvialis oil powder, 0.1 parts of grape seed extract, 1 part of microcrystalline cellulose, 1 part of cross-linked polyvinylpyrrolidone, 0.05 parts of povidone, 0.1 parts of silicon dioxide and 0.01 parts of magnesium stearate.

[0072] The preparation method of the above health food is as follows:

[0073] S1. Weigh astragalus, Acanthopanax, Salvia miltiorrhiza, and Gynostemma pentaphyllum according to the formula ratio, add water and reflux to extract twice, each time for 1.0 hour, add 11 times water each time, filter, and combine the filtrates to obtain an extract;

[0074] S2, the extract is concentrated under reduced pressure (concentration temperature is 80°C, vacuum degree is 0.03MPa) to a relative density of 1.05-1.15 (60°C) to obtain an extract, the extract is dried at a drying temperature of 120°C and a vacuum degree of 0.04Mpa to obtain a dry paste (water content ≤ 8%), the dry paste is crushed (sieve mesh number: 80 mesh), and the dry paste powder is collected;

[0075] S3, mixing the dry paste powder, Haematococcus pluvialis oil powder, grape seed extract and other raw and auxiliary materials, and preparing capsules according to a conventional method.

[0076] Example 8

[0077] A health food, comprising the composition with antioxidant function obtained in Example 3. The raw materials and auxiliary materials are as follows:

[0078] 5 parts of Astragalus, 1 part of Acanthopanax senticosus, 5 parts of Salvia miltiorrhiza, 1 part of Gynostemma pentaphyllum, 0.1 parts of Haematococcus pluvialis oil powder, 0.7 parts of grape seed extract, 2 parts of microcrystalline cellulose, 0.1 parts of cross-linked polyvinylpyrrolidone, 0.5 parts of povidone, 0.05 parts of silicon dioxide and 0.08 parts of magnesium stearate.

[0079] The preparation method of the above health food is as follows:

[0080] S1. Weigh astragalus, Acanthopanax, Salvia miltiorrhiza, and Gynostemma pentaphyllum according to the formula ratio, add water and reflux to extract twice, each time for 2.0 hours, add 9 times water each time, filter, and combine the filtrates to obtain an extract;

[0081] S2, the extract is concentrated under reduced pressure (concentration temperature is 50°C, vacuum degree is 0.09MPa) to a relative density of 1.05-1.15 (60°C) to obtain an extract, the extract is dried at a drying temperature of 110°C and a vacuum degree of 0.04Mpa to obtain a dry paste (water content ≤ 8%), the dry paste is crushed (sieve mesh number: 80 mesh), and the dry paste powder is collected;

[0082] S3, mixing the dry paste powder, Haematococcus pluvialis oil powder, grape seed extract and other raw and auxiliary materials, and preparing granules according to a conventional method.

[0083] Comparative Example 1

[0084] A composition, compared with Example 1, wherein grape seed extract is replaced by epigallocatechin gallate, and the composition is specifically made of the following raw materials in parts by weight: 3 parts of astragalus; 3 parts of acanthopanax senticosus; 3 parts of salvia miltiorrhiza; 3 parts of gynostemma pentaphyllum; 0.36 parts of Haematococcus pluvialis oil powder and 0.2 parts of epigallocatechin gallate.

[0085] The preparation method of the composition is as follows:

[0086] (1) Weigh astragalus, Acanthopanax senticosus, Salvia miltiorrhiza, and Gynostemma pentaphyllum according to the formula ratio, add water and reflux to extract twice, each time for 1.5 hours, add 10 times of water each time, filter, and combine the filtrates to obtain an extract;

[0087] (2) the extract is concentrated under reduced pressure (concentration temperature is 60° C., vacuum degree is 0.06 MPa) to a relative density of 1.05 to 1.15 (60° C.) to obtain an extract, the extract is dried at a drying temperature of 115° C. and a vacuum degree of 0.06 MPa to obtain a dry paste (water content ≤ 8%), the dry paste is crushed (sieve mesh number: 80 mesh), and the dry paste powder is collected;

[0088] (3) The obtained dry paste powder is mixed with epigallocatechin gallate and Haematococcus pluvialis oil powder to form the composition.

[0089] Comparative Example 2

[0090] A composition, compared with Example 1, wherein the grape seed extract is replaced with tomato red, and is specifically made of the following raw materials in parts by weight: 3 parts of Astragalus; 3 parts of Acanthopanax; 3 parts of Salvia miltiorrhiza; 3 parts of Gynostemma pentaphyllum; 0.36 parts of Haematococcus pluvialis oil powder and 0.2 parts of tomato red.

[0091] The preparation method of the composition is as follows:

[0092] (1) Weigh astragalus, Acanthopanax senticosus, Salvia miltiorrhiza, and Gynostemma pentaphyllum according to the formula ratio, add water and reflux to extract twice, each time for 1.5 hours, add 10 times of water each time, filter, and combine the filtrates to obtain an extract;

[0093] (2) the extract is concentrated under reduced pressure (concentration temperature is 60° C., vacuum degree is 0.06 MPa) to a relative density of 1.05 to 1.15 (60° C.) to obtain an extract, the extract is dried at a drying temperature of 115° C. and a vacuum degree of 0.06 MPa to obtain a dry paste (water content ≤ 8%), the dry paste is crushed (sieve mesh number: 80 mesh), and the dry paste powder is collected;

[0094] (3) The obtained dry paste powder is mixed with tomato red and Haematococcus pluvialis oil powder to form the composition.

[0095] Comparative Example 3

[0096] A composition, compared with Example 1, wherein grape seed extract is replaced by epigallocatechin gallate and lycopene, and the composition is specifically made of the following raw materials in parts by weight: 3 parts of astragalus; 3 parts of acanthopanax senticosus; 3 parts of salvia miltiorrhiza; 3 parts of gynostemma pentaphyllum; 0.36 parts of Haematococcus pluvialis oil powder; 0.2 parts of epigallocatechin gallate and 0.2 parts of lycopene.

[0097] The preparation method of the composition is as follows:

[0098] (1) Weigh astragalus, Acanthopanax senticosus, Salvia miltiorrhiza, and Gynostemma pentaphyllum according to the formula ratio, add water and reflux to extract twice, each time for 1.5 hours, add 10 times of water each time, filter, and combine the filtrates to obtain an extract;

[0099] (2) the extract is concentrated under reduced pressure (concentration temperature is 60° C., vacuum degree is 0.06 MPa) to a relative density of 1.05 to 1.15 (60° C.) to obtain an extract, the extract is dried at a drying temperature of 115° C. and a vacuum degree of 0.06 MPa to obtain a dry paste (water content ≤ 8%), the dry paste is crushed (sieve mesh number: 80 mesh), and the dry paste powder is collected;

[0100] (3) The obtained dry paste powder is mixed with epigallocatechin gallate, lycopene and Haematococcus pluvialis oil powder to form the composition.

[0101] Comparative Example 4

[0102] A composition, compared with Example 1, further comprises epigallocatechin gallate and lycopene, and is specifically made of the following raw materials in parts by weight: 3 parts of astragalus; 3 parts of acanthopanax senticosus; 3 parts of salvia miltiorrhiza; 3 parts of gynostemma pentaphyllum; 0.36 parts of Haematococcus pluvialis oil powder; 0.5 parts of grape seed extract; 0.2 parts of epigallocatechin gallate and 0.2 parts of lycopene.

[0103] The preparation method of the composition is as follows:

[0104] (1) Weigh astragalus, Acanthopanax senticosus, Salvia miltiorrhiza, and Gynostemma pentaphyllum according to the formula ratio, add water and reflux to extract twice, each time for 1.5 hours, add 10 times of water each time, filter, and combine the filtrates to obtain an extract;

[0105] (2) the extract is concentrated under reduced pressure (concentration temperature is 60° C., vacuum degree is 0.06 MPa) to a relative density of 1.05 to 1.15 (60° C.) to obtain an extract, the extract is dried at a drying temperature of 115° C. and a vacuum degree of 0.06 MPa to obtain a dry paste (water content ≤ 8%), the dry paste is crushed (sieve mesh number: 80 mesh), and the dry paste powder is collected;

[0106] (3) The obtained dry powder is mixed with grape seed extract, epigallocatechin gallate, lycopene and Haematococcus pluvialis oil powder to form the composition.

[0107] Test Example 1

[0108] In order to better verify the characteristics of the composition of the present invention, the composition of Example 1 and the compositions of Comparative Examples 1 to 7 were subjected to animal tests for antioxidant function.

[0109] Animal experiments were conducted in accordance with the antioxidant evaluation methods in the draft "Technical Guidelines for Testing and Evaluation of Health Food Functions" and "Methods for Testing and Evaluation of Health Food Functions (2022 Edition)".

[0110] 1 Materials and Methods

[0111] 1.1 Sample:

[0112] The recommended oral dosage of each formulation of the composition prepared according to Example 1 and Comparative Examples 1 to 4 of the present invention is 3.44 g, 3.14 g, 3.14 g, 3.34 g and 3.84 g per day, respectively. The adult body weight is 60 kg, and the equivalent dosage is 0.0573 g / kg·bw, 0.0523 g / kg·bw, 0.0523 g / kg·bw, 0.0557 g / kg·bw and 0.0640 g / kg·bw, respectively.

[0113] 1.2 Experimental Animals:

[0114] 160 SPF-grade ICR male mice aged 8-10 months were provided by Hunan Slake Jingda Experimental Animal Co., Ltd., with the experimental animal production license number SCXK(Xiang)2019-0004, and the feed was provided by Changsha Tianqin Biotechnology Co., Ltd.

[0115] 1.3 Experimental environment conditions:

[0116] The experimental conditions were a barrier environment. During the experiment, the temperature was 21°C to 24°C, the humidity was 50% to 58%, and the experimental animal use license number was SYXK (Xiang) 2020-0011.

[0117] 1.4 Main instruments and reagents:

[0118] Animal scales, constant temperature water bath, UV-2600 ultraviolet-visible spectrophotometer, centrifuge, etc.; malondialdehyde (MDA), protein carbonyl, superoxide dismutase (SOD), and reduced glutathione (GSH) test kits were provided by Nanjing Jiancheng Bioengineering Institute.

[0119] 1.5 Dose selection and sample processing:

[0120] According to the recommended oral dosage for human body, three dosage groups of Example 1 and Comparative Examples 1 to 4 (equivalent to 5, 10, and 30 times of the recommended oral dosage for human body, respectively) and a solvent control group were set. Each dosage group of the formula was prepared according to the following method:

[0121] Example 1: The low, medium and high doses are 0.287 g / kg·bw, 0.573 g / kg·bw and 1.720 g / kg·bw respectively. Take 2.87 g, 5.73 g and 17.20 g of the sample respectively and add purified water to make up to 200 ml;

[0122] Comparative Example 1: The low, medium and high doses were 0.262 g / kg·bw, 0.523 g / kg·bw and 1.570 g / kg·bw, respectively. 2.62 g, 5.23 g and 15.70 g of the sample were taken, respectively, and the volume was fixed to 200 ml with purified water;

[0123] Comparative Example 2: The low, medium and high doses were 0.262 g / kg·bw, 0.523 g / kg·bw and 1.570 g / kg·bw, respectively. 2.62 g, 5.23 g and 15.70 g of the sample were taken, respectively, and the volume was fixed to 200 ml with purified water;

[0124] Comparative Example 3: The low, medium and high doses were 0.278 g / kg·bw, 0.557 g / kg·bw and 1.670 g / kg·bw, respectively. 2.78 g, 5.57 g and 16.70 g of the sample were taken, respectively, and the volume was fixed to 200 ml with purified water;

[0125] Comparative Example 4: The low, medium and high doses were 0.320 g / kg·bw, 0.640 g / kg·bw and 1.920 g / kg·bw, respectively. 3.20 g, 6.40 g and 19.20 g of the sample were taken, respectively, and the volume was fixed to 200 ml with purified water;

[0126] Control group: given an equal volume of purified water.

[0127] The animals in each group were given oral administration with a volume of 0.2 ml / 10 g·bw, once a day for 30 consecutive days.

[0128] 1.6 Experimental Methods: Using Aged Animals

[0129] Before the experiment, the aged mice were weighed, and 20 μl of tail tip blood was collected and added with 0.48 mL of pure water to make a 4% hemolysate. The MDA level in the hemolysate was determined according to the instructions of the kit. The mice were divided into a control group and three dose groups according to the MDA level. The mice in each dose group were given different concentrations of the test solution according to the dosage design in 1.5, and the control group was given an equal volume of solvent for continuous gavage for 30 days. On the 31st day, the tail tip blood was collected to determine the content of malondialdehyde (MDA), a lipid peroxidation degradation product of 4% hemolysate, and the content of reduced glutathione (GSH), an antioxidant substance in whole blood; the eyeballs were removed and blood was collected and centrifuged, and serum was taken to determine the protein carbonyl content of protein oxidation products and the activity of the antioxidant enzyme superoxide dismutase (SOD).

[0130] 1.7 Experimental data statistics: Statistical analysis was performed using Excel and SPSS software. When SPSS software was used for analysis, the data were first tested for homogeneity of variance. If the variances were homogeneous, one-way analysis of variance was used for overall comparison. If differences were found, the Dunnett method was used for pairwise comparisons between the means of multiple dose groups and a control group. If the variances were not homogeneous, appropriate variable transformation was performed on the original data. After the test for homogeneity of variance was satisfied, the transformed data was used for statistics. If the purpose of homogeneity of variance was still not achieved after the variable transformation, the rank sum test was used for statistics. If the overall comparison was found to be different, the Tamhane's T2 test, which did not require homogeneity of variance, was used for pairwise comparisons.

[0131] 1.8 Result determination: If three of the four indicators, namely, lipid peroxide content, protein carbonyl, antioxidant enzyme activity, and reduced glutathione, are positive, it can be determined that the antioxidant animal test results of the test sample are positive.

[0132] 2 Results

[0133] 2.1 Effects on mouse body weight

[0134] As shown in Table 1, there was no statistical difference in the body weight and weight gain of mice in each dosage group at the initial, middle and final stages of the experiment compared with the control group (P>0.05).

[0135] Table 1 Effects on body weight of aged mice

[0136]

[0137] 2.2 Effects on 4% hemolysed lipid peroxide (MDA) in aged mice

[0138] The results of the effects of each group on 4% lysed hemoperoxide (MDA) in aged mice are shown in the following table.

[0139] Table 2 Effects on MDA content in aged mice

[0140]

[0141] Comparison between each dose group and the control group: The MDA level in the blood of mice in the high dose group of Example 1 at the end of the experiment was significantly lower than that in the control group, and the difference was statistically significant (P<0.05).

[0142] Comparison of the effects of reducing the level of MDA in the hemolyzed mice at the end of the experiment among the groups: Example 1 > Comparative Example 2 > Comparative Example 3 > Comparative Example 4 > Comparative Example 1.

[0143] 2.3 Effect on serum superoxide dismutase (SOD) activity in aged mice

[0144] The results of the effects of each group on the activity of serum superoxide dismutase (SOD) in aged mice are shown in the following table.

[0145] Table 3 Effects on serum SOD activity in aged mice.

[0146]

[0147]

[0148] Comparison between each dose group and the control group: The serum SOD activity of mice in the low-dose and high-dose groups of Example 1 was significantly higher than that in the control group, and the difference was significant (P<0.05).

[0149] Comparison of the effects of increasing serum SOD activity in mice among the groups: Example 1 > Comparative Example 1 > Comparative Example 2 > Comparative Example 3 > Comparative Example 4.

[0150] 2.4 Effects on the content of reduced glutathione (GSH) in whole blood of aged mice

[0151] The results of the effects of each group on the reduced glutathione (GSH) content in whole blood of aged mice are shown in the following table.

[0152] Table 4 Effects on whole blood GSH content in aged mice.

[0153]

[0154]

[0155] Compared with the control group, the whole blood GSH content of mice in the high dose group of comparative example 2, the medium and high dose groups of Example 1, and the low, medium and high dose groups of comparative example 1 were significantly higher than those in the control group, and the difference was significant (P<0.05).

[0156] Comparison of the effects of increasing the whole blood GSH content of mice among the groups: Comparative Example 1> Example 1> Comparative Example 2> Comparative Example 3> Comparative Example 4.

[0157] 2.5 Effects on the Carbonyl Content of Serum Protein in Aged Mice

[0158] The results of the effects of each group on the carbonyl content of serum protein in aged mice are shown in the following table.

[0159] Table 5 Effects on the carbonyl content of serum proteins in aged mice.

[0160]

[0161]

[0162] There was no significant difference in the serum protein carbonyl content between the mice in each dosage group and the control group (P>0.05).

[0163] There was no obvious trend in the comparison of the effects of reducing the carbonyl content of serum protein in mice among the groups.

[0164] 3 Summary

[0165] Under the laboratory conditions, different doses of the compositions of Example 1 and Comparative Examples 1 to 4 were orally administered to aged mice for 30 consecutive days to conduct an antioxidant function test.

[0166] The 0.287g / kg·bw dose of Example 1 can increase serum SOD activity, the 0.573g / kg·bw dose can increase whole blood GSH content, and the 1.720g / kg·bw dose can reduce the hemolytic MDA level, increase serum SOD activity, and increase whole blood GSH content. Compared with the control group, the difference is significant (P<0.05). According to the evaluation criteria in the "Antioxidant Function Evaluation Method" (National Food and Drug Administration

[2012] No. 107), it is determined that it has an antioxidant function for animals. It shows that Example 1 of the present invention has an antioxidant function for animals.

[0167] From the above data, it can be seen that the addition of grape seed extract to the basic formula of Chinese medicine Astragalus, Acanthopanax, Salvia miltiorrhiza, Gynostemma pentaphyllum plus Haematococcus pluvialis oil powder in the present invention can play a synergistic role in antioxidant activity, while the addition of lycopene and epigallocatechin gallate does not play a synergistic antioxidant role. It is speculated that the different antioxidant action mechanisms of the four components of Haematococcus pluvialis oil powder, grape seed extract, lycopene and epigallocatechin gallate and the different concentration ratios of the composite composition may affect the synergistic effect of the composite antioxidant, which needs to be further explored in the future.

[0168] The antioxidant mechanism of Haematococcus pluvialis oil powder in the present invention is mainly to quench singlet oxygen and remove free radicals; reduce membrane permeability and limit the penetration of oxidants into cells; increase the activity of antioxidant enzymes and inhibit lipid peroxidation. The antioxidant mechanism of grape seed extract (proanthocyanidins) is mainly to remove existing free radicals and inhibit the generation of free radicals. The antioxidant mechanism of lycopene is mainly to quench singlet oxygen; the antioxidant mechanism of epigallocatechin gallate is mainly to increase the activity of antioxidant enzymes or directly remove harmful peroxides such as free radicals. Different mechanisms of antioxidants will lead to different antioxidant interactions in different proportions or in different systems.

[0169] In addition, the type, concentration (ratio), and combination of antioxidants will affect the synergistic effect of compound antioxidants. Antioxidant interactions not only have antioxidant synergistic effects, but also antioxidant superposition and antioxidant antagonism. The change in the ratio of a certain active ingredient after compounding different antioxidants often leads to very different antioxidant interactions.

[0170] The composition provided by the present invention achieves an antioxidant synergistic effect by compounding specific ingredients in a specific ratio, and obtains an unexpected effect. The composition has excellent antioxidant function and can be used in the preparation of health foods, functional foods or medicines with antioxidant function to meet people's daily needs for health.

[0171] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modification, equivalent substitution or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A composition having an antioxidant function, It is characterized in that The invention comprises the following raw materials in parts by weight: 0.5-10 parts of astragalus, 0.5-10 parts of acanthopanax, 0.5-10 parts of salvia miltiorrhiza, 0.5-10 parts of gynostemma pentaphyllum, 0.05-1 parts of haematococcus pluvialis oil powder and 0.1-0.9 parts of grape seed extract.

2. The composition having antioxidant function as claimed in claim 1, It is characterized in that The invention comprises the following raw materials in parts by weight: 1-5 parts of astragalus, 1-5 parts of acanthopanax, 1-5 parts of salvia miltiorrhiza, 1-5 parts of gynostemma pentaphyllum, 0.1-0.5 parts of Haematococcus pluvialis oil powder and 0.2-0.7 parts of grape seed extract.

3. The composition with antioxidant function as claimed in claim 1, It is characterized in that The invention comprises the following raw materials in parts by weight: 10 parts of astragalus, 0.5 parts of acanthopanax senticosus, 10 parts of salvia miltiorrhiza, 0.5 parts of gynostemma pentaphyllum, 1 part of haematococcus pluvialis oil powder and 0.1 parts of grape seed extract.

4. The composition having antioxidant function as claimed in claim 1, It is characterized in that The invention comprises the following raw materials in parts by weight: 3 parts of astragalus, 3 parts of acanthopanax, 3 parts of salvia miltiorrhiza, 3 parts of gynostemma pentaphyllum, 0.36 parts of Haematococcus pluvialis oil powder and 0.5 parts of grape seed extract.

5. The composition with antioxidant function as claimed in claim 1, It is characterized in that The invention comprises the following raw materials in parts by weight: 5 parts of astragalus, 1 part of acanthopanax senticosus, 5 parts of salvia miltiorrhiza, 1 part of gynostemma pentaphyllum, 0.1 part of haematococcus pluvialis oil powder and 0.7 part of grape seed extract.

6. The composition having antioxidant function according to any one of claims 1 to 5, It is characterized in that The preparation method of the composition comprises the following steps: (1) Take Astragalus, Acanthopanax, Salvia miltiorrhiza, and Gynostemma pentaphyllum, add water and reflux to extract twice, filter, and obtain an extract; (2) concentrating the extract under reduced pressure to a relative density of 1.05 to 1.15 to obtain an extract, drying the extract to obtain a dry paste, and crushing the dry paste to obtain a dry paste powder; (3) The obtained dry paste powder, grape seed extract and Haematococcus pluvialis oil powder together constitute the composition.

7. The composition having antioxidant function as claimed in claim 6, It is characterized in that In step (1), each extraction is performed for 1.0 to 2.0 hours, and 9 to 11 times of water is added each time; in step (2), the temperature for reduced pressure concentration is 50 to 80° C., and the vacuum degree is 0.03 to 0.09 MPa; in step (2), the drying temperature is 110 to 120° C., and the vacuum degree is 0.04 to 0.10 MPa.

8. Use of the composition with antioxidant function according to any one of claims 1 to 5 in the preparation of health food, functional food or medicine.

9. A health food, It is characterized in that A composition with antioxidant function comprising the composition according to any one of claims 1 to 5.

10. The health food according to claim 9, It is characterized in that The invention also includes the following raw and auxiliary materials in parts by weight: 1 to 2 parts of microcrystalline cellulose; 0.1 to 1 part of cross-linked polyvinylpyrrolidone; 0.05 to 0.5 part of polyvinylpyrrolidone; 0.05 to 0.1 part of silicon dioxide and 0.01 to 0.08 part of magnesium stearate.

11. The health food according to claim 10, It is characterized in that The dosage form of the health food is granules, tablets or capsules.

12. The health food according to claim 11, It is characterized in that The preparation method of the health food tablet comprises the following steps: S1. Take Astragalus, Acanthopanax, Salvia miltiorrhiza, and Gynostemma pentaphyllum, add water and reflux to extract twice, filter, and obtain an extract; S2, decompressing and concentrating the extract to a relative density of 1.05 to 1.15 to obtain an extract, drying the extract to obtain a dry paste, and crushing the dry paste to obtain a dry paste powder; S3, mixing the dry paste powder, microcrystalline cellulose and grape seed extract, adding an ethanol solution of povidone K30 to prepare a soft material, granulating, and drying to obtain dry granules; S4, premixing the Haematococcus pluvialis oil powder and cross-linked polyvinylpyrrolidone to obtain a premixed powder, and mixing the premixed powder with the obtained dry granules, silicon dioxide, and magnesium stearate to obtain a total mixed granule; S5. Compressing and coating the obtained total mixed granules to obtain the product.

13. The health food according to claim 12, It is characterized in that In step S1, each extraction takes 1.0 to 2.0 hours, and 9 to 11 times of water is added each time; in step S2, the temperature for reduced pressure concentration is 50 to 80° C., and the vacuum degree is 0.03 to 0.09 MPa; in step S2, the drying temperature is 110 to 120° C., and the vacuum degree is 0.04 to 0.10 MPa.

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