Skin barrier maintenance, moisturizing essence and preparation method thereof

Abstract

The present invention provides a skin barrier maintenance, moisturizing essence and a preparation method thereof, and relates to the field of cosmetic technology. The essence of the present invention comprises: a composite polypeptide, a hyaluronic acid complex, a plant active extract, a moisturizer, an emollient, a thickener, a preservative and water; the plant active extract is composed of seaweed extract, dark green agave extract, coltsfoot flower extract and field mint extract. The composite polypeptide has the comprehensive effects of repairing the barrier, anti-aging and firming, brightening the skin tone and long-lasting moisturizing through the synergistic effect of multiple targets; the plant active extract has the effects of moisturizing, repairing the skin's physical barrier, anti-inflammatory, and relieving dryness and sensitivity. The experimental results show that there is a synergistic effect between the composite polypeptide and the plant active extract, and the essence obtained by compounding the two has excellent skin barrier maintenance and moisturizing effects.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a skin barrier maintenance and moisturizing essence and a preparation method thereof. Background Art

[0002] In modern life, our skin faces a variety of challenges. Environmental pollution, UV radiation, climate change, and improper skincare practices can all lead to damage to the skin barrier. Once the skin barrier is damaged, water loss is increased, leading to dryness, roughness, flaking, itching, and other problems, seriously affecting the skin's appearance and health.

[0003] Currently, there are numerous moisturizing products on the market designed to address dry, dehydrated skin. However, most focus solely on surface moisturization, forming a closed film on the skin's surface to reduce water evaporation. This effect is often short-lived and limited. These products typically fail to fundamentally repair a damaged skin barrier, failing to meet consumers' demand for long-lasting, deep-level skin care.

[0004] While some traditional moisturizing ingredients, such as glycerin and hyaluronic acid, can absorb and retain moisture to a certain extent, they are not very effective in improving the skin's barrier function. Furthermore, some products contain a high level of synthetic chemicals, which may irritate the skin and trigger adverse reactions such as allergies, further burdening the skin.

[0005] As people pay more and more attention to skin health, developing an essence product that can effectively maintain the skin barrier, achieve long-lasting moisturizing and is safe and gentle has become an urgent need in the skin care field. Summary of the Invention

[0006] The purpose of the present invention is to provide a skin barrier maintenance, moisturizing essence and a preparation method thereof. The essence of the present invention has excellent skin barrier repair and moisturizing effects.

[0007] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0008] The invention provides a composition for maintaining the skin barrier and moisturizing the skin, comprising the following raw materials in parts by weight: 1-3 parts of a composite polypeptide, 0.5-1 parts of a hyaluronic acid complex, and 5-10 parts of a plant active extract; the plant active extract is composed of a seaweed extract, a dark green agave extract, a coltsfoot flower extract, and a field mint extract in a weight ratio of 3-7:2-5:1-3:0.1-0.2.

[0009] Preferably, the composite polypeptide is two or more of oligopeptide-1, tripeptide-1 copper, nonapeptide-1 and palmitoyl hexapeptide-12.

[0010] Preferably, the hyaluronic acid complex is composed of hyaluronic acid with a molecular weight of 10KDa-100KDa and hyaluronic acid with a molecular weight of 400KDa-500KDa in a weight ratio of 5-8:1-5.

[0011] Preferably, the preparation method of the seaweed extract comprises: crushing the seaweed, mixing with sodium hydroxide solution, heating, centrifuging, taking the supernatant, concentrating and precipitating with alcohol to obtain the seaweed extract.

[0012] Preferably, the dark green agave extract is obtained by sequentially hydrolyzing with β-1,4-glucan-4-glucanohydrolase and serrapeptase.

[0013] Preferably, the coltsfoot flower extract is obtained by extraction with an ethanol solution.

[0014] Preferably, the wild mint extract is obtained by supercritical CO2 extraction.

[0015] The present invention also provides the use of the composition in preparing skin barrier maintenance and moisturizing cosmetics.

[0016] The present invention also provides a skin barrier maintenance and moisturizing essence containing the above composition, which is composed of the following components in parts by weight, based on 100 parts by weight: 5-15 parts of the composition, 1-5 parts of a moisturizer, 3-8 parts of an emollient, 0.1-0.5 parts of a thickener, 0.1-0.3 parts of a preservative, and water to make up to 100 parts.

[0017] The present invention also provides a preparation method of the above-mentioned skin barrier maintenance and moisturizing essence, which is characterized by comprising: mixing a thickener with water and heating it, and mixing it with a moisturizer to obtain an aqueous phase; heating and dissolving the moisturizer to obtain an oil phase; adding the oil phase to the aqueous phase, adding the composition and a preservative to obtain the skin barrier maintenance and moisturizing essence.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] The present invention provides a composition for skin barrier maintenance and moisturizing, comprising: a composite polypeptide, a hyaluronic acid complex, and plant active extracts; the plant active extracts being composed of seaweed extract, dark green agave extract, coltsfoot flower extract, and field mint extract. The composite polypeptide, through multi-target synergistic action, exhibits comprehensive benefits of barrier repair, anti-aging, firming, skin brightening, and long-lasting moisturizing. The plant active extracts also exhibit moisturizing, repair of the skin's physical barrier, anti-inflammatory, and relief of dryness and sensitivity. Experimental results demonstrate a synergistic effect between the composite polypeptide and the plant active extracts, resulting in an essence with excellent skin barrier maintenance and moisturizing effects. DETAILED DESCRIPTION

[0020] The invention provides a composition for maintaining the skin barrier and moisturizing the skin, comprising the following raw materials in parts by weight: 1-3 parts of a composite polypeptide, 0.5-1 parts of a hyaluronic acid complex, and 5-10 parts of a plant active extract; the plant active extract is composed of a seaweed extract, a dark green agave extract, a coltsfoot flower extract, and a wild mint extract in a weight ratio of 3-7:2-5:1-3:0.1-0.2.

[0021] The polypeptides in the composite polypeptide of the present invention are preferably two or more of oligopeptide-1, tripeptide-1 copper, nonapeptide-1 and palmitoyl hexapeptide-12, more preferably composed of oligopeptide-1, tripeptide-1 copper, nonapeptide-1 and palmitoyl hexapeptide-12 in a weight ratio of (0.5-1.5):(3-8):(1-3):(8-12), and the weight ratio is more preferably 1:5:2:10.

[0022] The oligopeptide-1 in the composite polypeptide of the present invention directly stimulates the proliferation of epidermal cells, accelerates the repair of damaged barriers (such as dryness, sensitivity, and micro-injury), and reduces transepidermal water loss. The tripeptide-1 copper activates the synthesis of collagen and glycosaminoglycans (such as hyaluronic acid), enhances the support of the dermis, and indirectly consolidates the epidermal barrier structure to form an "inside-out" barrier protection network; palmitoyl hexapeptide-12 stimulates fibroblasts to secrete collagen, elastin, and fibronectin, improving skin elasticity and firmness. The tripeptide-1 copper scavenges free radicals, inhibits the degradation of collagen by matrix metalloproteinases, and prolongs the anti-aging effect. The tripeptide-1 copper protects existing collagen, and the palmitoyl hexapeptide-12 promotes new collagen. The anti-aging pathway delays wrinkles and sagging; Nonapeptide-1 blocks the binding of melanocyte-stimulating hormone to melanocyte receptors, inhibits tyrosinase activity from the source, and reduces melanin production. Oligopeptide-1 + tripeptide-1 copper accelerates epidermal renewal (and reduces post-inflammatory pigmentation, assists in fading spots, and achieves a bright complexion through the three stages of "inhibiting production - accelerating metabolism - anti-inflammatory and anti-settling"; Palmitoyl hexapeptide-12 enhances the water storage capacity of the dermis, and oligopeptide-1 repairs the integrity of the stratum corneum, reduces water evaporation, enhances short-term moisturizing effects, and covers the moisturizing needs of multiple layers of skin. The composite polypeptide of the present invention can achieve the four-in-one comprehensive effects of "barrier repair - anti-aging and firming - brightening skin tone - long-lasting moisturizing" through the synergistic action of multiple targets.

[0023] The hyaluronic acid complex described herein is preferably composed of hyaluronic acid with a molecular weight of 10-100kDa and hyaluronic acid with a molecular weight of 400-500kDa, in a weight ratio of 5-8:1-5, more preferably 6:3. The hyaluronic acid with a molecular weight of 400-500kDa can instantly boost skin moisturization, while the hyaluronic acid with a molecular weight of 10-100kDa penetrates deep into the epidermis (stratum spinosum and stratum basale), enhancing dermal-epidermal water exchange. Combined with the complex polypeptide, it creates a multi-dimensional moisturizing system that locks moisture in the surface layer, penetrates the middle layer, and repairs deep layers.

[0024] The preparation method of the seaweed extract of the present invention preferably comprises: crushing the seaweed, mixing with sodium hydroxide solution and heating, centrifuging, taking the supernatant, concentrating and precipitating with alcohol, and precipitating as the seaweed extract. The concentration of the sodium hydroxide is preferably 0.8%-1.5%, more preferably 1%, and the mass volume ratio of the seaweed to the sodium hydroxide solution is preferably 1:20-30g / mL, more preferably 1:25g / mL; before heating, it is preferably included in ultrasonic treatment, the frequency is preferably 20-40kHz, more preferably 30kHz, the time is preferably 30-40min, more preferably 35min; the heating temperature is preferably 85-95℃, more preferably 90℃, and the time is preferably 40-60min, more preferably 50min. The seaweed extract obtained by the preparation method of the present invention can enhance the skin's water-locking ability, relieve dryness, and has the effects of deep moisturizing and barrier repair.

[0025] The dark green agave extract of the present invention is obtained by sequentially hydrolyzing with β-1,4-glucan-4-glucanohydrolase and serrapeptase. The preparation method of the dark green agave extract preferably comprises: drying and crushing dark green agave leaves, mixing with deionized water, adding β-1,4-glucan-4-glucanohydrolase for enzymatic hydrolysis, inactivating the enzyme, adding serrapeptase for enzymatic hydrolysis, inactivating the enzyme, filtering, and concentrating and drying the filtrate to obtain the dark green agave extract. The enzymatic activity of the β-1,4-glucan-4-glucanohydrolase is preferably 200,000-300,000 U / g, more preferably 250,000 U / g. The addition amount is preferably 2%-5%, more preferably 3%, of the dark green agave leaves by weight. The enzymatic hydrolysis temperature is preferably 33-37°C, more preferably 35°C, the pH is preferably 4.5-5.5, more preferably 5, and the hydrolysis time is preferably 30-40 minutes, more preferably 35 minutes. The serrapeptase activity is preferably 150,000-250,000 U / g, more preferably 200,000 U / g. The addition amount is preferably 1%-3% of the weight of the dark green agave leaves, more preferably 2%. The enzymatic hydrolysis temperature is preferably 45-55°C, more preferably 50°C, the pH value is preferably 6.5-7.5, more preferably 7, and the time is preferably 30-60 minutes, more preferably 40 minutes. The dark green agave extract prepared by the present invention is rich in natural moisturizing factors (such as polysaccharides and amino acids), can inhibit the decomposition of hyaluronic acid, enhance the water content of the stratum corneum, lock in moisture for a long time and repair the skin barrier, and has soothing, anti-allergic, antibacterial, anti-aging, anti-inflammatory, astringent and oil-control effects.

[0026] The coltsfoot flower extract of the present invention is obtained by extraction with an ethanol solution. The preparation method of the coltsfoot flower extract is preferably as follows: the coltsfoot flower is dried and crushed, mixed with a 50%-60% (v / v) ethanol solution, extracted at 60-70°C for 40-50min, filtered, and the filtrate is concentrated under reduced pressure and dried to obtain the coltsfoot flower extract. A more preferred preparation method is: the coltsfoot flower is dried and crushed, mixed with a 55% (v / v) ethanol solution, extracted at 65°C for 45min, filtered, and the filtrate is concentrated under reduced pressure and dried to obtain the coltsfoot flower extract. The coltsfoot flower extract obtained by the preparation method of the present invention has the effects of soothing, moisturizing, nourishing, tightening pores, and anti-aging.

[0027] The wild mint extract of the present invention is obtained by supercritical CO2 extraction. The preparation method of the wild mint extract preferably comprises: drying and crushing the field mint, supercritically extracting for 1-2 hours under the conditions of 15-20MPa, 50-65°C, and a CO2 flow rate of 25-40kg / h, collecting the extract, and obtaining the field mint extract. A more preferred preparation method is: drying and crushing the field mint, supercritically extracting for 1.5 hours under the conditions of 18MPa, 55°C, and a CO2 flow rate of 30kg / h, collecting the extract, and obtaining the field mint extract. The field mint extract prepared by the method of the present invention not only has the effects of shrinking pores, soothing and calming, and anti-inflammatory, but also can change the permeability of the skin, promote the transdermal absorption of other components, and thus better exert the effects of skin barrier maintenance and moisturizing.

[0028] The sea hyacinth extract and dark green agave extract in the plant active extracts of the present invention can enhance the density of the stratum corneum, promote the expression of FLG / LOR proteins and repair the physical barrier; the sea hyacinth extract and dark green agave extract form a bionic moisturizing layer, combined with the anti-inflammatory effect of coltsfoot flower extract, relieve dryness and sensitivity and reduce transepidermal water loss; the oil-control and astringent properties of wild mint extract and the water-oil balancing ability of dark green agave extract work synergistically to solve the barrier fragility problem of combination skin / oily skin.

[0029] The present invention also provides the use of the composition in preparing skin barrier maintenance and moisturizing cosmetics.

[0030] The present invention also provides a skin barrier maintenance and moisturizing essence containing the above composition, which is composed of the following components in parts by weight, based on 100 parts by weight: 5-15 parts of the composition, 1-5 parts of a moisturizer, 3-8 parts of an emollient, 0.1-0.5 parts of a thickener, 0.1-0.3 parts of a preservative, and water to make up to 100 parts.

[0031] The moisturizer of the present invention is preferably one or more of PEG / PPG-14 / 7 dimethyl ether, glyceryl glucoside and sodium 1,2-hexanediol; the emollient is preferably one or more of squalane, isononyl isononanoate and phenyl trimethicone; the thickener is preferably one or more of carbomer, dehydroxanthan gum and sclerotium gum; the preservative is preferably one or more of phenoxyethanol, chlorphenesin and salicylic acid.

[0032] The present invention also provides a preparation method of the above-mentioned skin barrier maintenance and moisturizing essence, comprising: mixing a thickener with deionized water and heating it, and mixing it with a moisturizer to obtain an aqueous phase; heating and dissolving the moisturizer to obtain an oil phase; adding the oil phase to the aqueous phase, adding the composition and a preservative to obtain the skin barrier maintenance and moisturizing essence.

[0033] The method of using the skin barrier maintenance and moisturizing essence of the present invention is as follows: after cleansing the skin, take 2-3 drops of essence, pour it into the palm of your hand, rub it with the temperature of your palm to warm it, and evenly apply it to the face, avoiding the skin around the eyes, and gently massage until absorbed. Apply once in the morning and evening every day.

[0034] In the present invention, unless otherwise specified, all raw material components are commercially available products well known to those skilled in the art.

[0035] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions of the present invention. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0036] In the following examples, unless otherwise specified, all methods are conventional.

[0037] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0038] Example 1

[0039] Composition for skin barrier maintenance and moisturizing

[0040] (1) Preparation of plant active extracts

[0041] The extract was dried and ground, passed through a 150-mesh sieve, mixed with 25 times the weight of a 1% (w / w) sodium hydroxide solution, ultrasonically treated at 30 kHz for 35 minutes, heated and stirred at 60° C. for 50 minutes, centrifuged, and the supernatant was collected. The extract was concentrated, precipitated with anhydrous ethanol, and vacuum-dried to a water content of 3 wt% to obtain a Hemiptera extract.

[0042] The dark green agave leaves are dried, crushed and passed through a 400-mesh sieve, mixed with 10 times the weight of deionized water, and β-1,4-glucan-4-glucanohydrolase is added to perform enzymatic hydrolysis at 35°C and pH 5 for 35 minutes to inactivate the enzyme. Serrapeptase is added to perform enzymatic hydrolysis at 50°C and pH 7 for 40 minutes to inactivate the enzyme, filtered, and the filtrate is concentrated and vacuum-dried to a water content of 4wt% to obtain a dark green agave extract; wherein the β-1,4-glucan-4-glucanohydrolase has an enzymatic activity of 250,000 U / g and is added in an amount of 3% by weight of the dark green agave leaves; and the serrapeptase has an enzymatic activity of 200,000 U / g and is added in an amount of 2% by weight of the dark green agave leaves.

[0043] The coltsfoot flower was dried and crushed, passed through a 200-mesh sieve, mixed with 8 times the weight of a 55% (v / v) ethanol solution, and stirred and extracted at 65° C. and 200 rpm for 45 min. The filtrate was filtered, concentrated, and vacuum-dried to a water content of 1 wt% to obtain a coltsfoot flower extract.

[0044] The mint was dried and crushed to pass through an 80-mesh sieve, and supercritical extraction was performed under the conditions of 18 MPa, 55°C, and a CO2 flow rate of 30 kg / h for 1.5 h, and the extract was collected to obtain the mint extract;

[0045] Mixing the seaweed extract, the dark green agave extract, the coltsfoot flower extract, and the wild mint extract in a weight ratio of 5:3:2:0.15 to obtain a plant active extract;

[0046] (2) Compositions for skin barrier maintenance and moisturizing

[0047] 2 parts by weight of the composite polypeptide, 0.8 parts by weight of the hyaluronic acid complex and 8 parts by weight of the plant active extract are mixed to obtain a composition for skin barrier maintenance and moisturizing;

[0048] The composite polypeptide is composed of oligopeptide-1, tripeptide-1 copper, nonapeptide-1 and palmitoyl hexapeptide-12 in a weight ratio of 1:5:2:10; the hyaluronic acid complex is composed of hyaluronic acid with a molecular weight of 60KDa and hyaluronic acid with a molecular weight of 450KDa in a weight ratio of 6:3.

[0049] Example 2

[0050] Composition for skin barrier maintenance and moisturizing

[0051] (1) Preparation of plant active extracts

[0052] The seaweed was dried and crushed, passed through a 100-mesh sieve, mixed with 20 times the weight of a 0.8% (w / w) sodium hydroxide solution, ultrasonically treated at 20 kHz for 40 minutes, heated and stirred at 55° C. for 60 minutes, centrifuged, the supernatant was collected, concentrated, and precipitated with anhydrous ethanol. The precipitate was vacuum-dried to a water content of 4wt% to obtain a seaweed extract;

[0053] The dark green agave leaves are dried, crushed and passed through a 300-mesh sieve, mixed with 8 times the weight of deionized water, and β-1,4-glucan-4-glucanohydrolase is added to perform enzymatic hydrolysis at 33° C. and pH 4.5 for 40 minutes to inactivate the enzyme. Serrapeptase is added to perform enzymatic hydrolysis at 45° C. and pH 6.5 for 30 minutes to inactivate the enzyme, filtered, and the filtrate is concentrated and vacuum-dried to a water content of 3% by weight to obtain a dark green agave extract; wherein the β-1,4-glucan-4-glucanohydrolase has an enzymatic activity of 200,000 U / g and is added in an amount of 5% by weight of the dark green agave leaves; and the serrapeptase has an enzymatic activity of 150,000 U / g and is added in an amount of 3% by weight of the dark green agave leaves.

[0054] The coltsfoot flower was dried and ground, passed through a 150-mesh sieve, mixed with 5 times the weight of a 50% (v / v) ethanol solution, and stirred and extracted at 70°C and 150 rpm for 40 minutes. The mixture was filtered, and the filtrate was concentrated and vacuum-dried to a water content of 3 wt% to obtain a coltsfoot flower extract.

[0055] The mint was dried and crushed to pass through a 50-mesh sieve, and supercritical extraction was performed under the conditions of 15 MPa, 65°C, and a CO2 flow rate of 25 kg / h for 1 hour, and the extract was collected to obtain the mint extract;

[0056] Mixing the seaweed extract, the dark green agave extract, the coltsfoot flower extract, and the wild mint extract in a weight ratio of 3:2:1:0.1 to obtain a plant active extract;

[0057] (2) Compositions for skin barrier maintenance and moisturizing

[0058] Mixing 1 part by weight of the composite polypeptide, 0.5 parts by weight of the hyaluronic acid complex, and 5 parts by weight of the plant active extract to obtain a composition for skin barrier maintenance and moisturizing;

[0059] The composite polypeptide is composed of oligopeptide-1, tripeptide-1 copper, nonapeptide-1 and palmitoyl hexapeptide-12 in a weight ratio of 0.5:3:1:8; the hyaluronic acid complex is composed of hyaluronic acid with a molecular weight of 10KDa and hyaluronic acid with a molecular weight of 400KDa in a weight ratio of 5:1.

[0060] Example 3

[0061] Composition for skin barrier maintenance and moisturizing

[0062] (1) Preparation of plant active extracts

[0063] The seaweed was dried and crushed, passed through a 200-mesh sieve, mixed with 30 times the weight of a 1.5% (w / w) sodium hydroxide solution, ultrasonically treated at 40 kHz for 30 minutes, heated and stirred at 75° C. for 40 minutes, centrifuged, the supernatant was collected, concentrated, and precipitated with anhydrous ethanol. The precipitate was vacuum-dried to a water content of 5wt% to obtain a seaweed extract;

[0064] The dark green agave leaves are dried, crushed and passed through a 500-mesh sieve, mixed with 12 times the weight of deionized water, and β-1,4-glucan-4-glucanohydrolase is added to perform enzymatic hydrolysis at 37°C and pH 5.5 for 30 minutes to inactivate the enzyme. Serrapeptase is added to perform enzymatic hydrolysis at 55°C and pH 7.5 for 20 minutes to inactivate the enzyme, filtered, and the filtrate is concentrated and vacuum-dried to a water content of 3wt% to obtain a dark green agave extract; wherein the β-1,4-glucan-4-glucanohydrolase has an enzymatic activity of 300,000 U / g and is added in an amount of 2% by weight of the dark green agave leaves; and the serrapeptase has an enzymatic activity of 250,000 U / g and is added in an amount of 1% by weight of the dark green agave leaves;

[0065] The coltsfoot flower was dried and crushed, passed through a 200-mesh sieve, mixed with 10 times the weight of a 60% (v / v) ethanol solution, and stirred and extracted at 60°C and 250 rpm for 50 min. The filtrate was filtered, concentrated, and vacuum-dried to a water content of 2 wt% to obtain a coltsfoot flower extract.

[0066] The mint was dried and crushed to pass through a 100-mesh sieve, and supercritical extraction was performed at 20 MPa, 50°C, and a CO2 flow rate of 40 kg / h for 2 h, and the extract was collected to obtain the mint extract;

[0067] Mixing the seaweed extract, the dark green agave extract, the coltsfoot flower extract, and the wild mint extract in a weight ratio of 7:5:3:0.2 to obtain a plant active extract;

[0068] (2) Compositions for skin barrier maintenance and moisturizing

[0069] Mixing 3 parts by weight of the composite polypeptide, 1 part by weight of the hyaluronic acid complex, and 10 parts by weight of the plant active extract to obtain a composition for skin barrier maintenance and moisturizing;

[0070] The composite polypeptide is composed of oligopeptide-1, tripeptide-1 copper, nonapeptide-1 and palmitoyl hexapeptide-12 in a weight ratio of 1.5:8:3:12; the hyaluronic acid complex is composed of hyaluronic acid with a molecular weight of 100KDa and hyaluronic acid with a molecular weight of 500KDa in a weight ratio of 8:5.

[0071] Example 4

[0072] Skin barrier maintenance, moisturizing essence

[0073] Weigh 10 parts by weight of the composition of Example 1, 3 parts by weight of glyceryl glucoside, 5 parts by weight of squalane, 0.3 parts by weight of carbomer, 0.2 parts by weight of phenoxyethanol, and add deionized water to make up to 100 parts by weight;

[0074] Carbomer was mixed with deionized water, heated and dissolved at 73°C and 350 rpm, glyceryl glucoside was added and mixed, and stirred at 350 rpm for 15 minutes to obtain an aqueous phase; squalane was heated and dissolved at 70°C to obtain an oil phase; the oil phase was added to the aqueous phase, stirred at 2000 rpm for 20 minutes, and then the composition and phenoxyethanol were added, the pH value was adjusted to 6, stirred at 3000 rpm for 25 minutes, vacuum degassed and sterilized to obtain a skin barrier maintenance and moisturizing essence.

[0075] Example 5

[0076] Skin barrier maintenance, moisturizing essence

[0077] Weigh 5 parts by weight of the composition of Example 2, 5 parts by weight of PEG / PPG-14 / 7 dimethyl ether, 3 parts by weight of isononyl isononanoate, 0.5 parts by weight of sclerotium gum, 0.1 parts by weight of salicylic acid, and make up to 100 parts with deionized water;

[0078] The sclerotium gum was mixed with deionized water, heated and dissolved at 70°C and 300 rpm, and then mixed with PEG / PPG-14 / 7 dimethyl ether, and stirred at 300 rpm for 20 minutes to obtain an aqueous phase; isononyl isononanoate (oil phase) was added to the aqueous phase, stirred at 1500 rpm for 25 minutes, and then the composition and salicylic acid were added. The pH value was adjusted to 5.8, and the mixture was stirred at 2500 rpm for 30 minutes. The mixture was vacuum degassed and sterilized to obtain a skin barrier maintenance and moisturizing essence.

[0079] Example 6

[0080] Skin barrier maintenance, moisturizing essence

[0081] Weigh 15 parts by weight of the composition of Example 3, 1 part by weight of sodium 1,2-hexanediol, 3 parts by weight of phenyl trimethicone, 0.1 part by weight of dehydrogenated xanthan gum, 0.3 part by weight of chlorphenesin, and make up to 100 parts by weight with deionized water;

[0082] Dehydroxanthan gum was mixed with deionized water, heated and dissolved at 75°C and 400 rpm, 1,2-hexanediol sodium was added, and the mixture was stirred at 400 rpm for 15 minutes to obtain an aqueous phase; phenyl trimethicone (oil phase) was added to the aqueous phase, stirred at 2500 rpm for 15 minutes, and then the composition and chlorphenesin were added. The pH value was adjusted to 7.2, and the mixture was stirred at 3500 rpm for 20 minutes. The mixture was vacuum degassed and sterilized to obtain a skin barrier maintenance and moisturizing essence.

[0083] Comparative Example 1

[0084] The difference from Example 1 is that the preparation method of the dark green agave extract is as follows: the dark green agave leaves are dried, crushed through a 400-mesh sieve, mixed with 10 times the weight of deionized water, and semi-β-1,4-glucan-4-glucanohydrolase is added for enzymatic hydrolysis at 45°C and pH 5.5 for 35 minutes, the enzyme is inactivated, papain is added for enzymatic hydrolysis at 55°C and pH 6.5 for 40 minutes, the enzyme is inactivated, filtered, the filtrate is concentrated, and vacuum dried to a water content of 4wt% to obtain a dark green agave extract; wherein the enzymatic activity of the semi-β-1,4-glucan-4-glucanohydrolase is 250,000 U / g, and the amount added is 3% of the weight of the dark green agave leaves; the enzymatic activity of the papain is 200,000 U / g, and the amount added is 2% of the weight of the dark green agave leaves.

[0085] Comparative Example 2

[0086] The difference from Example 1 is that “2 parts by weight of composite polypeptide, 0.8 parts by weight of hyaluronic acid complex and 8 parts by weight of plant active extract” is replaced by “0.8 parts by weight of hyaluronic acid complex and 10 parts by weight of plant active extract”.

[0087] Comparative Example 3

[0088] The difference from Example 1 is that "2 parts by weight of complex polypeptide, 0.8 parts by weight of hyaluronic acid complex and 8 parts by weight of plant active extract" is replaced by "10 parts by weight of complex polypeptide and 0.8 parts by weight of hyaluronic acid complex".

[0089] Comparative Example 4

[0090] The difference from Example 4 is that the composition of Example 1 is replaced by the composition of Comparative Example 1.

[0091] Comparative Example 5

[0092] The difference from Example 4 is that the composition of Example 1 is replaced by the composition of Comparative Example 2.

[0093] Comparative Example 6

[0094] The difference from Example 4 is that the composition of Example 1 is replaced by the composition of Comparative Example 3.

[0095] Test Example 1

[0096] Cell scratch assay

[0097] The compositions of Examples 1-3 and Comparative Examples 1-3 were respectively diluted with DMEM culture medium to prepare dispersions with a concentration of 1% (w / w).

[0098] HaCat cells (MlBio / ELISA) in the logarithmic growth phase were digested, resuspended, and counted. 6×10 5 Cells / well were cultured (37°C, 5% CO2, 24h), and blank group, Example 1-3 group, and Comparative Example 1-3 group were set up respectively. After the cells were 100% fused, the tip of the gun was used to measure the glass slide, as close as possible to the horizontal scratch on the back. The tip of the gun should be vertical and not tilted. After the scratching, the cells were washed 3 times with PBS, the scratched cells were removed, and DMEM culture medium (blank group), 1% (w / w) dispersion of the composition of Example 1-3 and Comparative Example 1-3 were added respectively. Placed in a 37°C, 5% CO2 incubator, cultured for 24 hours, samples were taken, photos were taken, and the repair rate was calculated. The repair rate = [1-(scratch area after 24h of culture / initial scratch area)] × 100%. The specific results are shown in Table 1.

[0099] Table 1 Skin barrier repair rate of each group

[0100]

[0101] As shown in Table 1, the skin barrier repair rates of the compositions of Examples 1-3 and Comparative Examples 1-3 were significantly improved compared to the blank control, with Examples 1-3 showing a higher skin barrier repair rate than Comparative Examples 1-3. A comparison of the data from Example 1 and Comparative Example 1 shows that the skin barrier repair effects of the compositions obtained by combining dark green agave extracts using different enzymatic hydrolysis enzymes differ. A comparison of the data from Example 1 and Comparative Examples 2-3 shows that the combination of composite polypeptides and plant active extracts significantly enhances skin barrier repair.

[0102] Test Example 2

[0103] Skin barrier maintenance, skin feel and moisturizing tests

[0104] Sixty female volunteers aged 30-45 years with sensitive skin were selected and randomly divided into 6 groups. There was no significant difference in age, skin condition, etc. among the groups, so relevant assessments could be conducted.

[0105] After cleansing the skin, take 3 drops of the essence of Examples 4-6 and Comparative Examples 4-6 respectively, pour them into the palm of your hand, rub them with the temperature of your palm to warm them, and evenly apply them to the face, avoiding the skin around the eyes, and gently massage until absorbed. Apply once in the morning and evening.

[0106] After 30 days of continuous use, the skin barrier improvement and skin feel were evaluated, and the skin water retention rate was measured. The specific results are shown in Table 2.

[0107] The scoring criteria for skin barrier improvement are: particularly obvious improvement in skin barrier, 5 points; obvious improvement in skin barrier, 4 points; not obvious improvement in skin barrier, 3 points; no improvement in skin barrier, 2 points; sensitization phenomenon, 1 point.

[0108] The skin feel rating standard is: very comfortable, refreshing and moisturizing without being sticky, and without rubbing into mud, 5 points; relatively comfortable, without being sticky, moisturizing with an oily feeling, and without rubbing into mud, 4 points; fairly comfortable, relatively moisturizing, and basically without rubbing into mud, 3 points; not very comfortable, with a sticky feeling or basically no moisturizing feeling, a floating oily feeling, and occasional rubbing into mud, 2 points; very uncomfortable, sticky, oily, not well absorbed, and with rubbing into mud, 1 point.

[0109] The method for measuring the skin's moisture retention rate is: after using essence and allowing the facial moisture to dry naturally, use a skin tester to measure the facial skin moisture content A at room temperature of 25°C and humidity of 60%. Sit quietly indoors for 1 hour, and then measure the facial skin moisture content B again under the same conditions to calculate the moisture retention rate: Moisture retention rate (%) = B ÷ A × 100%.

[0110] Table 2 Skin barrier improvement and skin feel scores of each group

[0111]

[0112] As shown in Table 2, the essences of Examples 4-6 are more effective than those of Comparative Examples 4-6 in improving skin barrier function, skin feel, and moisturizing. The data from Example 4 and Comparative Example 4 indicate that the essences obtained by compounding dark green agave extracts using different enzymes have different effects on improving skin barrier function, skin feel, and moisturizing. The data from Example 4 and Comparative Examples 5-6 indicate that the composite polypeptides of the present invention and the active plant extracts have a synergistic effect in improving skin barrier function, skin feel, and moisturizing.

[0113] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A composition for skin barrier maintenance and moisturizing, characterized in that: The invention is prepared from the following raw materials in parts by weight: 1-3 parts of composite polypeptide, 0.5-1 parts of hyaluronic acid complex and 5-10 parts of plant active extract; The plant active extract is composed of seaweed extract, dark green agave extract, coltsfoot flower extract and field mint extract in a weight ratio of 3-7:2-5:1-3:0.1-0.2; The dark green agave extract is obtained by enzymatic hydrolysis of β-1,4-glucan-4-glucanohydrolase and serrapeptase in sequence; The composite polypeptide is composed of oligopeptide-1, tripeptide-1 copper, nonapeptide-1 and palmitoyl hexapeptide-12 in a weight ratio of (0.5-1.5):(3-8):(1-3):(8-12); The preparation method of the seaweed extract comprises: crushing the seaweed, mixing with a sodium hydroxide solution, heating, centrifuging, taking the supernatant, concentrating and precipitating with alcohol to obtain the seaweed extract; The coltsfoot flower extract is obtained by extraction with an ethanol solution; The wild mint extract is obtained by supercritical CO2 extraction.

2. The composition according to claim 1, characterized in that The hyaluronic acid complex is composed of hyaluronic acid with a molecular weight of 10KDa-100KDa and hyaluronic acid with a molecular weight of 400KDa-500KDa in a weight ratio of 5-8:1-5.

3. Use of the composition according to any one of claims 1 to 2 in the preparation of skin barrier maintenance and moisturizing cosmetics.

4. A skin barrier maintenance and moisturizing essence containing the composition according to any one of claims 1-2, characterized in that: The invention is composed of the following components in parts by weight, based on 100 parts by weight: 5-15 parts of the composition, 1-5 parts of the moisturizing agent, 3-8 parts of the emollient, 0.1-0.5 parts of the thickener, 0.1-0.3 parts of the preservative and water to make up to 100 parts.

5. The method for preparing the skin barrier maintenance and moisturizing essence according to claim 4, characterized in that: include: The thickener is mixed with water and heated, and then mixed with a moisturizer to obtain a water phase; the emollient is heated and dissolved to obtain an oil phase; The oil phase is added to the water phase, and the composition and preservatives are added to obtain a skin barrier maintenance and emollient moisturizing essence.

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