Aeromonas sp. And application thereof

Abstract

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to an aeromonas sp. And application thereof. The Aeromonas sanarellai is classified and named as Aeromonas sanarellai, the strain number is P1-5, the Aeromonas sanarellai is preserved in the China General Microbiological Culture Collection Center on October 31, 2024, and the preservation number is CGMCC No.32451, and the Aeromonas sanarellai is preserved in the China General Microbiological Culture Collection Center on October 31, 2024. The nucleotide sequence of the 16S rDNA (ribosomal deoxyribonucleic acid) of the aeromonas Rayleiana is as shown in SEQ ID NO. 1; the strain has nitrogen fixing, phosphorus dissolving and growth promoting properties, can normally grow under the culture condition of 2% NaCl, and provides a novel microbial resource for treatment of coastal saline-alkali soil; the strain can also significantly increase the absorption of nutrient elements such as nitrogen, phosphorus and the like, enhance the salt resistance and stress resistance of plants and promote the growth of the plants, and has wide application prospects in the aspects of saline-alkali soil improvement and plant growth promotion and stress resistance.

Description

Technical Field

[0001] The invention belongs to the technical field of agricultural microorganisms, and specifically relates to an Aeromonas St. Reilly strain and application thereof. Background Art

[0002] Coastal saline-alkali soil is one of the main types of saline-alkali soil. However, due to its high salt content and low soil fertility, coastal saline-alkali soil limits plant growth and significantly reduces agricultural productivity. Excessive salt content in the soil will destroy the ion homeostasis in plant cells, hinder the plant's absorption of required nutrients, and have a toxic effect on the plant's root system and growth and development. Therefore, the rational use and improvement of saline-alkali land is of great significance to ensuring my country's food security.

[0003] Common existing methods for improving saline-alkali land include irrigation and salt removal, application of chemical improvers, organic fertilizers, etc., but these methods are usually costly and may have negative impacts on the environment. The use of biological methods to improve saline-alkali soil, especially the application of microbial remediation technology in saline-alkali soil, has received widespread attention in recent years. Studies have shown that some specific microbial groups, such as nitrogen-fixing bacteria, phosphate-dissolving bacteria, potassium-dissolving bacteria, etc., can effectively improve the soil environment, enhance the tolerance of plants to saline-alkali environments, and promote plant growth. They have shown great application potential in the improvement of saline-alkali land and increasing crop yields.

[0004] At present, the microorganisms that have been found to be able to be used for saline-alkali land improvement are mainly Bacillus, Pseudomonas, Halomonas and other species with salt-alkali tolerance. The potential of Aeromonas in saline-alkali land improvement has been gradually explored, among which the salt-alkali tolerance and plant growth promotion functions of bivalvium species have been proven. However, there are no reports on the research of Aeromonas sanarellii in saline-alkali land improvement. Therefore, it is of great scientific significance and practical value to develop Aeromonas sanarellii with strong salt-alkali adaptability and explore its application in saline-alkali land improvement and agricultural production. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a strain of Aeromonas St. Reilly P1-5 for improving coastal saline-alkali soil and its application in view of the deficiencies of the prior art. The strain has strong salt tolerance and multiple growth-promoting characteristics, including nitrogen fixation, phosphorus solubilization, siderophore production, indoleacetic acid (IAA) production, etc., and can also significantly reduce soil salt content, increase the content of available phosphorus, promote plant absorption of nutrients, enhance plant salt tolerance and stress resistance, thereby promoting plant growth.

[0006] In order to solve the above technical problems, the present invention discloses a strain of Aeromonas sanarellii, classified and named Aeromonas sanarellii, with strain number P1-5, which was deposited in the General Microbiological Center of China National Microbiological Culture Collection Administration on October 31, 2024, with the deposit number CGMCC No.32451.

[0007] The 16S rDNA nucleotide sequence of Aeromonas st.

[0008] SEQ ID NO.1:

[0009]

[0010] Among them, the Aeromonas St. Reilly is a Gram-negative bacterium, and the surface of the colony is smooth and round, light yellow and opaque.

[0011] The Aeromonas St. Reilly has the functions of fixing nitrogen and dissolving inorganic phosphorus.

[0012] Among them, the Aeromonas St. Reilly has the plant stress resistance and growth promotion functions of secreting iron carriers and producing indoleacetic acid.

[0013] Furthermore, the application of the above-mentioned Aeromonas St. Reilly in the improvement of coastal saline-alkali soil is also within the protection scope of the present invention;

[0014] Specifically, the application is the application of Aeromonas St. Reilly to reduce soil salinity in coastal saline-alkali soil and / or the application of Aeromonas St. Reilly to increase soil available phosphorus in coastal saline-alkali soil.

[0015] Furthermore, the use of the above-mentioned Aeromonas St. Reilly in promoting plant growth in coastal saline-alkali soil is also within the protection scope of the present invention;

[0016] Specifically, the application includes at least one of the following applications:

[0017] The application of Aeromonas St. Reilly in promoting the growth of plants such as biomass, vine length, stem thickness, etc. in coastal saline-alkali soil;

[0018] The application of Aeromonas Saint-Reilly in coastal saline-alkali soil to promote the absorption of nitrogen and phosphorus nutrients by plants;

[0019] Aeromonas St. Reilly reduces Na in plants in coastal saline-alkali soil + / K + Ratio, application to increase plant salt tolerance and stress resistance;

[0020] Preferably, the above-mentioned plants include but are not limited to sweet potatoes.

[0021] Furthermore, the present invention also provides a coastal saline-alkali soil improver, wherein the improver contains the above-mentioned Aeromonas St. Reilly;

[0022] Specifically, in some embodiments of the present invention, the Aeromonas St. Reilly was inoculated into LB liquid culture medium, and cultured at 30°C and 180r / min for 12h to prepare a seed solution, and then inoculated into LB liquid culture medium at a 1% v / v inoculation amount, and cultured for 24h to obtain a bacterial solution (~10 8CFU / ml); after the sweet potato seedlings grew normally for 5 days, each pot was irrigated with 50mL of bacterial solution, and the control group was irrigated with the same amount of sterile culture medium. After 14 days, the bacterial solution and the same amount of sterile culture medium were applied again, and the soil salt content and available phosphorus content after the sweet potato was harvested were measured, indicating that the St. Reilly Aeromonas has a good ability to reduce salt and dissolve phosphorus in coastal saline-alkali soil, proving the application potential of St. Reilly Aeromonas in the preparation of coastal saline-alkali soil conditioners.

[0023] Furthermore, a plant growth promoter in coastal saline-alkali soil, the growth promoter contains the above-mentioned Aeromonas St. Reilly;

[0024] Specifically, in some embodiments of the present invention, the Aeromonas St. Reilly was inoculated into LB liquid culture medium, and cultured at 30°C and 180r / min for 12h to prepare a seed solution, and then inoculated into LB liquid culture medium at a 1% v / v inoculation amount, and cultured for 24h to obtain a bacterial solution (~10 8 CFU / ml); After the sweet potato seedlings grew normally for 5 days, each pot was watered with 50mL of bacterial solution, and the control group was watered with the same amount of sterile culture medium. After 14 days, the bacterial solution and the same amount of sterile culture medium were applied again. The aboveground biomass, vine length, stem diameter, total phosphorus, total potassium, and Na content of the sweet potatoes after harvest were measured. + / K was measured, indicating that the Aeromonas St. Reilly can significantly promote the growth of sweet potatoes in coastal saline-alkali soil, demonstrating the application potential of Aeromonas St. Reilly in the preparation of plant growth promoters in coastal saline-alkali soil.

[0025] The above-mentioned coastal saline-alkali soil refers to a moderate coastal saline-alkali soil with a soluble salt content of 2 to 4 g / kg.

[0026] Beneficial effects:

[0027] (1) The present invention discovered and preserved for the first time a salt-alkali tolerant strain Aeromonas Sanrellis P1-5, which has the ability to fix nitrogen, dissolve phosphorus and promote growth, and can grow normally under 2% NaCl culture conditions, providing a new type of microbial resource for the treatment of coastal saline-alkali soil.

[0028] (2) Multiple functions and high efficiency: The strain P1-5 provided by the present invention not only has the effect of reducing salt-soluble phosphorus in coastal saline-alkali soil, but also can significantly increase the absorption of nutrients such as nitrogen and phosphorus, enhance the salt tolerance and stress resistance of plants, and promote plant growth. This strain has broad application prospects in saline-alkali land improvement and plant growth promotion and stress resistance.

[0029] (3) Environmental friendliness: The strain P1-5 provided by the present invention is an indigenous microorganism screened from coastal saline-alkali soil. It is easy to adapt to the soil environment, has the characteristics of low cost and green environmental protection, and will not cause pollution to the environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments, and the above and / or other advantages of the present invention will become more clear.

[0031] Figure 1 The colony morphology of the strain P1-5 of the present invention and its morphology under a microscope;

[0032] Figure 2 The cell morphology of the strain P1-5 of the present invention under a microscope after Gram staining;

[0033] Figure 3 The effect of the strain P1-5 of the present invention on the salt content and available phosphorus content of coastal saline-alkali soil;

[0034] Figure 4 The effect of the strain P1-5 of the present invention on the growth indexes of sweet potatoes in coastal saline-alkali soil;

[0035] Figure 5 The strain P1-5 of the present invention has an effect on the nitrogen and phosphorus nutrients and Na + / K + impact. DETAILED DESCRIPTION

[0036] The experimental methods described in the following examples are conventional methods unless otherwise specified; the reagents and materials described are commercially available unless otherwise specified.

[0037] Example 1: Isolation, screening and identification of strain P1-5

[0038] (1) Rhizosphere soil collection: Crop rhizosphere soil was collected from soils of different salinities at Jinhai Farm, Dafeng District, Yancheng (120.493°E, 32.594°N). The collected rhizosphere soil was placed in ziplock bags and brought back to the laboratory and stored at 4°C.

[0039] (2) Initial screening of strains: After passing the soil sample through a 10-mesh sieve (pore size of about 2 mm), 5 g of rhizosphere soil sample was weighed in a sterile clean bench and placed in a conical flask containing 45 ml of sterile water (7 to 8 sterilized glass beads were placed in the flask), and shaken on a shaker at 180 rpm and a constant temperature of 25°C for 30 min. After standing for 10 min, 10 -1 Use a pipette to draw 0.5 ml of 10 -1 The sample dilution was placed in a centrifuge tube containing 4.5 ml of sterile water and shaken to obtain 10 -2 According to this concentration gradient method, the sample dilution is gradually diluted to 10 -3 , 10 -4 , 10 -5 , 10 -6Sample dilution. Take 100ul of each gradient and spread it on beef extract peptone solid culture medium, and repeat each concentration three times. After placing the plate in a 30℃ constant temperature incubator and inverting it for 48 hours, pick single colonies of different forms on the solid culture medium and inoculate them on new beef extract peptone solid culture medium. Observe the growth of the strain every 24 hours, purify it more than 5 times, until it grows into a single colony with good growth condition, inoculate the single colony on beef extract peptone solid culture medium and store it in a 4℃ refrigerator for later use;

[0040] The beef extract peptone solid culture medium formula is: peptone 10g, sodium chloride 5g, beef extract powder 3g, agar 15g, water 1L, pH 7.2-7.4; sterilized at 121°C for 20min.

[0041] (3) Strain rescreening: In order to screen salt-tolerant microorganisms, all the initially screened bacteria were inoculated into basic sterile LB liquid medium for activation in batches, and beef extract peptone solid medium with NaCl content of 2%, 4%, 6%, 8%, and 10% (wt%) was prepared respectively. The activated strains were streaked on plates and cultured at 30°C for 24 to 48 hours. The growth of the strains was observed and the results were recorded to determine the salt tolerance of the strains. Colonies grown within 48 hours were recorded as tolerant, and no colonies were recorded as intolerant;

[0042] The formula of the beef extract peptone solid culture medium containing NaCl is as follows: 10 g peptone, 5 g sodium chloride, 3 g beef extract powder, 15 g agar, 1 L water, followed by adding 2%, 4%, 6%, 8%, 10% (wt%) NaCl, pH 7.2-7.4; sterilizing at 121°C for 20 min;

[0043] The formula of the LB liquid culture medium is: 10 g of tryptone, 5 g of yeast extract, 10 g of NaCl, 1 L of water, pH 7.0; sterilized at 121°C for 20 min.

[0044] (4) Strain purification and storage: The isolated and purified strain was inoculated into 25 ml LB liquid culture medium and cultured overnight at 30°C and 150 r / min. The cultured bacterial liquid was mixed with 50% glycerol at a ratio of 1:1 in a 2 ml centrifuge tube and stored in a -80°C ultra-low temperature refrigerator.

[0045] (5) Morphological identification of strains: The purified strain P1-5 was selected and cultured on beef extract peptone solid medium by the plate streak method at 30°C for 1 day. The colony morphology was observed. Figure 1 The colony is smooth, round, light yellow, opaque, and easy to pick up. Microscopic observation shows that it is a Gram-negative bacterium ( Figure 2 ).

[0046] (6) Molecular biological identification of strains: DNA was extracted from the strain P1-5 obtained by screening, and the strain was amplified using the bacterial universal primers 27F (SEQ ID No. 2: 5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (SEQ ID No. 3: 5'-TACGGTTACCTTGTTACGACTT-3') as a template. After amplification, the strain was sequenced by Nanjing Jisi Huiyuan Biotechnology Co., Ltd. The 16S rDNA nucleotide sequence obtained by sequencing is shown in SEQ ID NO. 1. The sequenced sequence was compared for homology in the NCBI database (https: / / blast.ncbi.nlm.nih.gov / Blast.cgi), and the results showed that the similarity between strain P1-5 and Aeromonas sanarellii strain HAE1 was as high as 99.65%. Therefore, it was identified that the present invention obtained a strain of Aeromonas sanarellii P1-5 through separation and purification.

[0047] (7) Strain preservation: Aeromonas Saint-Reilly P1-5 was sent to the General Microbiology Center of China Microorganism Culture Collection Administration (CGMCC) for preservation on October 31, 2024, and was classified and named Aeromonas asanaerllii, with the strain number P1-5 and the preservation number CGMCC No.32451. The preservation address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0048] Example 2: Analysis of growth-promoting properties of strain P1-5

[0049] The isolated single colony of strain P1-5 was transferred to a conical flask containing 25 mL of liquid culture medium and cultured at 30°C and 150 r / min for 24 h to obtain seed solution. The ability of strain P1-5 to fix nitrogen, dissolve inorganic phosphorus, dissolve potassium, produce siderophores, produce indoleacetic acid (IAA), and produce 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase was determined.

[0050] (1) Nitrogen fixation ability test: Take 10uL of seed liquid and inoculate it on Ashby nitrogen-free medium. Incubate it upside down in a 30℃ constant temperature incubator for 7 days to observe whether the colony can grow normally on the nitrogen-free medium.

[0051] (2) Inorganic phosphorus solubility test: 10 μL of seed solution was applied to the inorganic phosphorus solid culture medium and incubated upside down in a 30°C constant temperature incubator for 7 days to observe whether there was a degradation transparent zone around the colony.

[0052] (3) Potassium-dissolving ability test: 10 μL of seed solution was applied to a silicate bacterial culture medium and incubated upside down in a 30°C constant temperature incubator for 7 days to observe whether a degradation transparent zone was formed on the culture medium.

[0053] (4) Iron carrier production capacity test: Take 10uL of seed solution and spot it on the CAS test medium. Incubate it upside down at 30℃ in a constant temperature incubator for 7 days and observe whether there is a yellow halo on the medium.

[0054] (5) IAA production capacity test: Take 0.5 ml of seed liquid and add it to 25 ml of liquid culture medium (the liquid culture medium is a mixture of YN liquid culture medium and 2.5 mg / mL tryptophan in a ratio of 4:1), shake and culture at 30°C, 180 r / min for 48 hours, take 1 ml of culture medium after 48 hours, centrifuge at 4°C, 8000 r / min for 10 minutes, take 200 ul of supernatant after centrifugation, add 400 ul of Salkowski colorimetric reagent, stand in the dark for 30 minutes, and observe whether a color reaction occurs. If red appears, it means that the plant has the ability to produce IAA.

[0055] (6) ACC deaminase production capacity test: 0.5 ml of seed liquid was inoculated into 25 ml of YN liquid medium and cultured at 30°C and 150 r / min for 3 days. Then 0.5 ml of culture medium was transferred into 5 ml of DF and 5 ml of ADF liquid medium respectively. Each medium was cultured for one day. After centrifugation, the absorbance of the supernatant was measured at 540 nm. If the growth of the strain in ADF medium was significantly better than that in DF medium, it indicated that the strain had the ability to produce ACC deaminase.

[0056] The above-mentioned Ashby nitrogen-free medium is composed of: 0.2 g of potassium dihydrogen phosphate, 0.2 g of magnesium sulfate, 0.2 g of sodium chloride, 5.0 g of calcium carbonate, 10.0 g of mannitol, 0.1 g of calcium sulfate, 15 g of agar, and 1 L of deionized water, pH 6.9-7.1.

[0057] The inorganic phosphorus solid culture medium is composed of: 10.0 g of glucose, 0.5 g of ammonium sulfate, 0.5 g of yeast extract powder, 0.3 g of sodium chloride, 0.3 g of potassium chloride, 0.3 g of magnesium sulfate, 0.03 g of ferrous sulfate, 0.03 g of manganese sulfate, 5.0 g of tricalcium phosphate, 15 g of agar, and 1 L of deionized water, pH 7.0-7.5; sterilized at 121°C for 15 min.

[0058] The silicate bacterial culture medium is composed of: 5.0 g sucrose, 0.5 g magnesium sulfate, 0.1 g calcium carbonate, 2.0 g disodium hydrogen phosphate, 0.005 g ferric chloride, 1.0 g glass powder, 15.0 g agar, 1 L deionized water, pH 6.8-7.2; sterilized at 121°C for 15 min.

[0059] The composition of the CAS detection medium is: chrome azuro blue S 0.0605g, hexadecyltrimethylammonium bromide 0.0729g, ferric chloride hexahydrate 0.00265g, sodium dihydrogen phosphate dihydrate 0.295g, sodium dihydrogen phosphate dodecahydrate 1.214g, ammonium chloride 0.125g, potassium dihydrogen phosphate 0.0375g, sodium chloride 0.0625g, agar 15g, deionized water 1L, pH 6.7-6.9; sterilized at 115°C for 30min.

[0060] The YN liquid culture medium is composed of: 10.0 g sucrose, 0.5 g yeast extract, 0.1 g sodium chloride, 1.0 g ammonium sulfate, 1.0 g dipotassium hydrogen phosphate, 0.5 g magnesium sulfate heptahydrate, 1 L deionized water, pH 7.2; sterilized at 121°C for 20 min.

[0061] The preparation method of Salkowaki color developer is as follows: add 7.5mL 0.5mol / L ferric chloride hexahydrate to 150mL sulfuric acid, and then add 250mL deionized water.

[0062] The above-mentioned DF liquid culture medium is composed of: 4 g potassium dihydrogen phosphate, 6 g disodium hydrogen phosphate, 0.2 g magnesium sulfate heptahydrate, 2 g glucose, 2 g gluconic acid, 2 g citric acid, 0.01 mg boric acid, 0.0112 mg magnesium sulfate, 0.1246 mg zinc sulfate, 0.0782 mg copper sulfate, 0.01 mg molybdenum trioxide, 1.0 mg ferrous sulfate heptahydrate, and 1 L deionized water; sterilize at 121°C for 20 min.

[0063] The above-mentioned ADF liquid culture medium is composed of: 4 g potassium dihydrogen phosphate, 6 g disodium hydrogen phosphate, 0.2 g magnesium sulfate heptahydrate, 2 g glucose, 2 g gluconic acid, 2 g citric acid, 5.0 mmol / L ACC mother solution, 0.01 mg boric acid, 0.0112 mg magnesium sulfate, 0.1246 mg zinc sulfate, 0.0782 mg copper sulfate, 0.01 mg molybdenum trioxide, 1.0 mg ferrous sulfate heptahydrate, and 1 L deionized water; sterilize at 121°C for 20 min.

[0064] The results of the strain growth-promoting ability test showed (Table 1) that strain P1-5 had good nitrogen fixation, inorganic phosphorus solubilization, siderophore production and IAA production abilities, while strain P1-5 had no significant effects on potassium solubilization and ACC deaminase production.

[0065] Table 1 Growth-promoting ability of strain P1-5

[0066] strain Nitrogen fixation Dissolved Inorganic Phosphorus Potassium Siderophore production Produced by IAA ACC deaminase P1-5 + + - + + -

[0067] Note: “+” means “yes”, “-” means “no”.

[0068] Example 3 Application of strains to reduce salt and dissolve phosphorus and promote growth in coastal saline-alkali soil

[0069] (1) Potted experiment: The test soil was collected from the Dongtai Tiaozini Reclamation Area in Yancheng City, Jiangsu Province. The soil type is coastal saline-alkali soil, with a soil pH of 8.99, a salt content of 2.38 g / kg, an organic matter content of 4.92 g / kg, a total nitrogen content of 0.33 g / kg, an available phosphorus content of 17.69 mg / kg, and an available potassium content of 242.50 mg / kg. The soil was air-dried and sieved (2 mm) before being placed in plastic pots with a diameter of 13 cm and a height of 12 cm, with 2 kg per pot. The test plant was Xushu 51, and two sweet potato seedlings of the same growth were grafted into each pot. The experiment was set up with two treatments: no bacteria (CK) and a single inoculation of bacterial solution (P1-5), and each treatment was repeated 3 times.

[0070] (2) Preparation of bacterial solution: strain P1-5 was inoculated into 25 mL LB liquid medium, and cultured at 30°C and 180 r / min for 12 h to obtain seed solution, which was then inoculated into 200 mL LB liquid medium at a 1% inoculum amount and cultured for 24 h before use (~10 8 CFU / ml).

[0071] (3) After the sweet potato seedlings have grown normally for 5 days, each pot is irrigated with 50 mL of bacterial solution, and the control group is irrigated with the same amount of sterile culture medium. After 14 days, 50 mL of bacterial solution and the same amount of sterile culture medium are applied again. During this period, watering and weeding are carried out on time. The potted plants are placed in the greenhouse of the Institute of Agricultural Resources and Environment of Jiangsu Academy of Agricultural Sciences for growth. In the later stage, watering is carried out in a timely and appropriate amount to maintain the soil moisture at 60% of the field water holding capacity. The sweet potatoes are harvested and sampled after 42 days of growth to measure the aboveground biomass, vine length, stem diameter, total phosphorus, total potassium, and Na content of the sweet potatoes. + / K + , as well as soil salinity and available phosphorus content. Figures 3 to 5 shown.

[0072] Compared with the uninoculated control, the application of P1-5 bacterial solution can significantly reduce the salt content of coastal saline-alkali soil by 14.5% and significantly increase the available phosphorus content in the soil by 6.3% ( Figure 3 ), indicating that strain P1-5 has a good ability to reduce salt and dissolve phosphorus in coastal saline-alkali soil.

[0073] Compared with the uninoculated control, the application of P1-5 bacterial solution significantly increased the aboveground biomass dry weight of sweet potato by 42.9%, and the vine length and stem diameter of sweet potato significantly increased by 37.4% and 34.4%, respectively ( Figure 4 ), indicating that strain P1-5 can significantly promote the growth of sweet potato in coastal saline-alkali soil.

[0074] Compared with the uninoculated control, the application of P1-5 bacterial solution significantly increased the total nitrogen and total phosphorus contents of the plants by 8.3% and 23.5%, respectively. + / K+ The ratio was significantly reduced by 37.6% ( Figure 5 ), indicating that strain P1-5 can not only significantly promote the absorption of nitrogen and phosphorus nutrients by sweet potatoes and improve the mineral nutrition status of sweet potatoes, but also + / K + Increase the salt tolerance and stress resistance of sweet potatoes and alleviate the adverse effects of salt on the growth of sweet potatoes.

[0075] In summary, the Aeromonas sanarellii strain selected from coastal saline-alkali soil in this study, numbered P1-5, has the ability to fix nitrogen, dissolve phosphorus, produce siderophores and produce IAA. The results of the sweet potato pot experiment showed that in a saline-alkali environment, strain P1-5 can not only effectively reduce soil salinity and increase the available phosphorus content in the soil, but also significantly improve the growth indicators of sweet potatoes such as biomass, vine length, and stem diameter, improve the nutritional status of the plants, increase the absorption of nitrogen and phosphorus nutrients, and reduce the Na in the plants. + / K + ratio, which enhances the salt tolerance of sweet potatoes.

[0076] The present invention provides a strain of Aeromonas St. Reilly and its application ideas. There are many methods and ways to implement the technical solution. The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention. All components not specified in this embodiment can be implemented by existing technologies.

Claims

1. A strain of Aeromonas St. Reilly, characterized in that: The classification name is Aeromonas sanarellii, the strain number is P1-5, and it was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on October 31, 2024, with the deposit number CGMCC No.32451.

2. The Aeromonas Saint-Reilly according to claim 1, characterized in that The 16SrDNA nucleotide sequence of Aeromonas St. Reilly is shown in SEQ ID NO.

1.

3. The Aeromonas Saint-Reilly according to claim 2, characterized in that The Aeromonas St. Reilly is a Gram-negative bacterium, and the surface of the colony is smooth and round, light yellow and opaque.

4. The Aeromonas Saint-Reilly according to claim 3, characterized in that The Aeromonas St. Reilly has the functions of fixing nitrogen and dissolving inorganic phosphorus.

5. The Aeromonas Saint-Reilly according to claim 4, characterized in that The Aeromonas Saint-Reilly has the plant stress resistance and growth promotion functions of secreting iron carriers and producing indoleacetic acid.

6. Use of Aeromonas St. Reilly as described in any one of claims 1 to 5 in improving coastal saline-alkali soil.

7. Use of Aeromonas St. Reilly as claimed in any one of claims 1 to 5 in promoting plant growth in coastal saline-alkali soil.

8. A coastal saline-alkali soil conditioner, characterized in that: The improving agent contains the Aeromonas St. Reilly described in any one of claims 1 to 5.

9. A plant growth promoter in coastal saline-alkali soil, characterized in that: The growth promoter contains the Aeromonas St. Reilly described in any one of claims 1 to 5.

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