Odor-removing and disinfecting spray composition as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of biomedical treatment, and particularly discloses an odor-removing and disinfecting spray composition as well as a preparation method and application thereof. The odor-removing and disinfecting spray composition is prepared from 8-12 parts of agilawood extract, 4-6 parts of lemon eucalyptus leaf extract, 3-5 parts of rosemary extract, 2-3 parts of polysorbate-80, 40-60 parts of ethanol, 20-30 parts of deionized water, small molecule peptide and a novel organic compound. The composition is used for home furnishing, clothes, in-car and other scenes, the composition is uniformly sprayed to a target area by pressing a spray head to eliminate peculiar smell and inhibit microbial growth, the home furnishing scenes comprise living rooms, bedrooms, kitchens, toilets and the like, the clothes scenes are stored in seasons or used when peculiar smell exists after long-time wearing, and the clothes scenes are used when peculiar smell exists after long-time wearing. The in-vehicle scene is used for removing peculiar smell, cigarette smell, food residual smell and the like of a new vehicle.

Description

Technical Field

[0001] The present invention belongs to the fields of biological medicine and chemical technology, and particularly relates to a sustainable antibacterial and deodorizing composition, a preparation method thereof, and an application thereof. Background Art

[0002] In daily life and many specific scenarios, such as hospital wards, gym locker rooms, school dormitories, etc., the problems of bacterial growth and odor emission are extremely prominent. In hospital wards, it is easy for germs to cross-infect, and the odors of medicines and the excrement of patients are mixed; the gym locker room is humid and stuffy, and the long-term residue of sweat causes a large number of bacteria to multiply, producing a pungent sour smell; the space in school dormitories is relatively small and crowded, and the odors emitted by clothes, shoes and socks and microbial contamination seriously affect the living environment and the health of students.

[0003] The disadvantages of traditional antibacterial and deodorizing means are significant. Although sodium hypochlorite-containing disinfectants have strong bactericidal power, they are often highly irritating, may be harmful to human health after volatilization, and are also prone to corrode the surface of items; ultraviolet sterilization has irradiation dead angles, cannot cover all directions, and has poor effects on some bacterial spores; activated carbon adsorbs odors without selectivity, has a limited capacity, needs to be replaced frequently, and has a high cost. With the increasing demand for a healthy and comfortable living environment, there is an urgent need to develop an efficient, safe and sustainable antibacterial and deodorizing solution to meet the usage requirements of diverse scenarios. Summary of the Invention

[0004] The present invention provides a deodorizing and disinfecting spray composition, comprising the following components in parts by weight: 8-12 parts of agarwood extract, 4-6 parts of lemon eucalyptus leaf extract, 3-5 parts of rosemary extract, 2-3 parts of polysorbate-80, 40-60 parts of ethanol, and 20-30 parts of deionized water, wherein the agarwood extract is obtained from natural agarwood by supercritical CO 2 extraction method, the lemon eucalyptus leaf extract is prepared by washing, drying and crushing fresh lemon eucalyptus leaves, using ethanol as a solvent, ultrasonically extracting at 40-50 °C for 2-3 hours, concentrating the extraction solution by rotary evaporation and drying under vacuum, and the rosemary extract is obtained by crushing dry rosemary, using water as a solvent, performing steam distillation for 2-3 hours, collecting the distillate, separating the liquid, and drying with anhydrous sodium sulfate.

[0005] Furthermore, it also includes the small amino acid peptide SEQ NO:15 - Lys - Pro - Cys - Trp - Arg - Gly - His (KPCWRGH) with an addition amount of 0.5 - 1 part. The small amino acid peptide is used to inhibit bacterial spores in the air, and its mechanism of action is as follows: The Cys residue binds to the special protein structure on the spore surface through the formation of a disulfide bond. The Lys and Arg residues carry positive charges and attract the negatively charged groups on the spore surface. The Pro residue increases the rigidity of the peptide chain to better fit with the irregular structure on the spore surface. The Trp and His residues utilize aromaticity and hydrophobicity to interact with the hydrophobic region on the spore surface.

[0006] Furthermore, it also includes the small amino acid peptide SEQ NO:16 - Glu - Asp - Val - Cys - Met - Tyr - Lys (EDVCMYK) with an addition amount of 0.8 - 1.2 parts. The small amino acid peptide is used to inhibit the growth of molds, and its mechanism of action is as follows: The Cys residue undergoes a chemical reaction with the sulfur - containing group on the mold cell wall. The Glu and Asp residues carry negative charges and bind to the cations on the mold cell surface. The Val and Met residues provide a hydrophobic environment for the peptide chain to insert into the lipid bilayer of the mold cell membrane. The aromatic ring structure of the Tyr residue binds to the relevant receptors on the mold cell surface. The Lys residue carries a positive charge to enhance the electrostatic adsorption and penetration ability to the mold cell.

[0007] Furthermore, it also includes two novel unreported small amino acid peptides SEQ NO:15 and SEQ NO:16. The addition amount of SEQ NO:15 is 0.4 - 0.6 part, and the addition amount of SEQ NO:16 is 0.6 - 0.8 part. The two act synergistically to inhibit the complex microbial - polluted environment. SEQ NO:15 mainly attacks bacterial spores, and SEQ NO:16 inhibits molds. SEQ NO:15 destroys the spore structure to promote the release of nutrients, and part of them can be absorbed by the mold cells damaged under the action of SEQ NO:16. SEQ NO:16 inhibits the stimulating effect of secondary metabolites such as toxins produced by molds on the resuscitation of bacterial spores, and vice versa.

[0008] Furthermore, it also includes the organic compound ethyl 4 - (2 - hydroxypropylamino) benzoate (abbreviation: HPAE) with an addition amount of 1 - 2 parts. The organic compound is synthesized by reacting ethyl p - aminobenzoate with propylene oxide under the action of a basic catalyst. During the synthesis process, the temperature is controlled at 60 - 70 °C, and the pH value is maintained at 9 - 10. The ester group in its molecular structure has lipophilicity to penetrate the microbial cell membrane. The amino group undergoes an acid - base neutralization reaction with the acidic groups inside the microbial cell. The hydroxyl group undergoes an esterification reaction or a condensation reaction with the functional groups in the odor molecules and forms hydrogen bonds with the proteins and enzyme molecules on the microbial surface.

[0009] Furthermore, it also includes the organic compound 3-(methylthio)-1-propanol (abbreviated as MTP), with an addition amount of 0.8 - 1.5 parts. The organic compound is synthesized by reacting 3-chloro-1-propanol and sodium methyl mercaptide as raw materials in a polar solvent at 40 - 50 °C. The mercapto group in the molecule has reducibility and the coordination ability with metal ions, undergoes redox reactions with the oxidative components in the odor molecules, binds to the active center of the metal enzyme in the microbial cells, and the hydroxyl group helps the compound dissolve and disperse in the aqueous environment.

[0010] Furthermore, it also includes the organic compound ethyl 2-(4-chlorophenoxy)-acetate (abbreviated as CPAE), with an addition amount of 1.2 - 2.2 parts. The organic compound is synthesized by carrying out an esterification reaction of 4-chlorophenol and ethyl chloroacetate as raw materials under the catalysis of concentrated sulfuric acid at 80 - 90 °C. The ester group can penetrate the microbial cell membrane, be hydrolyzed by esterase after entering the cell, and the released 4-chlorophenoxy and acetate ions change the intracellular environment. The phenoxy group enhances the hydrophobicity of the molecule, and the chlorine atom enhances the acidity of the phenoxy group, acting on the basic groups on the microbial surface. At the same time, CPAE can also undergo nucleophilic substitution reactions with the nucleophiles in the odor molecules.

[0011] Furthermore, it also includes two new unreported organic compounds: 5-amino-2-(methylthio)-benzimidazole (abbreviated as AMBI) and 3-bromo-2-hydroxypropyl acrylate (abbreviated as BHPA). The addition amount of AMBI is 0.6 - 1 part, and the addition amount of BHPA is 0.8 - 1.2 parts. AMBI is synthesized by cyclization reaction at high temperature (180 - 200 °C) with o-phenylenediamine and methionine as raw materials under the catalysis of polyphosphoric acid. The amino group can bind to biological macromolecules such as nucleic acids and proteins in microbial cells, and the sulfur atom can coordinate with metal ions on the surface of microbial cells. BHPA is synthesized by esterification reaction at 60 - 70 °C with 3-bromo-1,2-propanediol and acrylic acid as raw materials under the catalysis of concentrated sulfuric acid. The bromine atom has strong electrophilicity and reacts with nucleophilic groups in microbial cells. The ester group and hydroxyl group can chemically react with odor molecules. The two work synergistically. AMBI focuses on inhibiting the physiological processes inside microorganisms, and BHPA acts on the microbial cell membrane and participates in the transformation of odor molecules. Also included is the organic compound 2-(4-chlorophenoxy)-ethyl acetate (abbreviated as CPAE) with an addition amount of 1.2 - 2.2 parts. The organic compound is synthesized by esterification reaction at 80 - 90 °C with 4-chlorophenol and ethyl chloroacetate as raw materials under the catalysis of concentrated sulfuric acid. The ester group can penetrate the microbial cell membrane and is hydrolyzed by esterase after entering the cell, releasing 4-chlorophenoxy and acetate. The organic compounds: 5-amino-2-(methylthio)-benzimidazole (abbreviated as AMBI) and 3-bromo-2-hydroxypropyl acrylate (abbreviated as BHPA). The addition amount of AMBI is 0.6 - 1 part, and the addition amount of BHPA is 0.8 - 1.2 parts. AMBI is synthesized by cyclization reaction at high temperature (180 - 200 °C) with o-phenylenediamine and methionine as raw materials under the catalysis of polyphosphoric acid. The amino group can bind to biological macromolecules such as nucleic acids and proteins in microbial cells, and the sulfur atom can coordinate with metal ions on the surface of microbial cells. BHPA is synthesized by esterification reaction at 60 - 70 °C with 3-bromo-1,2-propanediol and acrylic acid as raw materials under the catalysis of concentrated sulfuric acid. The bromine atom has strong electrophilicity and reacts with nucleophilic groups in microbial cells. The ester group and hydroxyl group can chemically react with odor molecules. The two work synergistically. AMBI focuses on inhibiting the physiological processes inside microorganisms, and BHPA acts on the microbial cell membrane and participates in the transformation of odor molecules.

[0012] Furthermore, the method for the agarwood odor-removing spray composition is characterized by including the following steps:

[0013] Preparing agarwood extract: Crushing natural agarwood into an appropriate particle size, placing it into the extraction kettle of a supercritical CO 2 extraction device, setting the extraction temperature at 40 - 50 °C, the extraction pressure at 20 - 30 MPa, the CO 2 flow rate at 20 - 30 L / h, extracting for 2 - 3 hours, and after completion, decompressing and separating to make CO 2 vaporize and escape to obtain the agarwood extract. Through high-performance liquid chromatography analysis, ensure that the content of the main active ingredients meets the standard and the purity reaches more than 80%;

[0014] Preparing plant extract:

[0015] Eucalyptus citriodora leaf extract: After treating fresh Eucalyptus citriodora leaves according to the above steps, add 5-8 times the amount of ethanol, and perform ultrasonic extraction at 40-50°C with an ultrasonic power of 300-400W. Intermittently for 10-15 minutes every 30-40 minutes, repeat 2-3 times. After completion, rotate and evaporate to concentrate and remove ethanol, and vacuum dry to constant weight. After gas chromatography detection, the content of cineole is not less than 60%; Rosemary extract: Crush dried rosemary and put it into a steam distillation device, add water and heat to boiling, and distill gently for 2-3 hours. Collect the distillate, separate the liquid, and dry it with anhydrous sodium sulfate. After high performance liquid chromatography determination, the total content of carnosic acid and rosmarinic acid is not less than 30%;

[0016] Preparation of Agarwood Odor-Removing Spray Composition:

[0017] At normal temperature and pressure, first add polysorbate-80 to part of deionized water, stir at 300-400 revolutions per minute for 10-15 minutes until fully dissolved to form an aqueous phase;

[0018] Then add agarwood extract, Eucalyptus citriodora leaf extract, and rosemary extract to ethanol in sequence, and stir at 200-300 revolutions per minute at 40-50°C for 20-30 minutes to fully dissolve each extract to form an oil phase;

[0019] Then slowly drip the oil phase into the aqueous phase, stirring at 400-500 revolutions per minute while dripping. After dripping, continue to stir for 30-40 minutes to ensure that the emulsion is fully emulsified and uniform;

[0020] Finally, make up to the specified weight with the remaining deionized water, stir evenly, filter and sterilize through a 0.22-0.45μm microporous membrane filter, and fill into a container with a spray pump head to obtain the agarwood odor-removing spray composition.

[0021] Furthermore, the application of the agarwood odor-removing spray composition in odor removal and antibacterial aspects, characterized in that the composition is used in scenarios such as homes, clothes, and cars. By pressing the nozzle of the sprayer, the composition is evenly sprayed on the target area to eliminate odors and inhibit the growth of microorganisms. Home scenarios include living rooms, bedrooms, kitchens, bathrooms, etc. The clothing scenario is used when storing clothes for the changing season or when there is an odor after long-term wearing. The car scenario is used to remove odors such as new car odors, cigarette smells, and food residue smells.

[0022] Advantages of the invention:

[0023] Excellent antibacterial efficacy: Multiple components work synergistically to build a strong antibacterial defense line. The active ingredients in plant extracts can damage the bacterial cell wall and cell membrane. The natural antibacterial agent chitosan interferes with bacterial metabolism. Organic compounds react with key functional groups of bacteria to inhibit their physiological functions. After testing, against common Escherichia coli, Staphylococcus aureus, etc., the antibacterial rate is over 90%, far exceeding traditional antibacterial products. Notably, the addition of new amino acid small peptides and organic compounds further broadens the antibacterial spectrum. For stubborn bacterial spores and molds, the inhibitory effect is significantly improved, effectively avoiding the problem of bacterial drug resistance and providing a reliable guarantee for long-term antibacterial. Outstanding odor removal performance: Plant extracts have a natural fragrance. While cleverly masking odors, their active ingredients can also chemically react with odor molecules to decompose or transform the root cause of odors. Organic compounds react with odor molecules such as ammonia, aldehydes, and thiols to form stable substances, reducing the generation and emission of odors from the source. Practical tests show that the removal rate of sweat odor, etc. can reach over 85%, with a long-lasting effect, keeping the usage scenario fresh and pleasant for a long time. High safety: Plant extracts are derived from nature, have good biocompatibility, and are non-irritating to the human body. The carefully designed and synthesized organic compounds fully consider safety factors, with no obvious toxicity and irritation. This makes the product particularly suitable for daily use by the elderly, children, pregnant women, and people sensitive to chemical substances, comprehensively protecting the health of all groups. Long-lasting durability: With the help of a polymer organic compound carrier, the active ingredients are slowly released. Even after multiple washes, the product still maintains good antibacterial and odor removal performance, reducing the trouble of frequent replacement and disposal, presenting users with a long-lasting and worry-free usage experience, and having extremely broad application prospects in multiple scenarios such as clothing, home, and car, highlighting its commercial value. Detailed implementation methods

[0024] Example 1

[0025] 1. Weigh 8 parts of agarwood extract, 4 parts of lemon eucalyptus leaf extract, 3 parts of rosemary extract, 2 parts of polysorbate-80, 40 parts of ethanol, and 20 parts of deionized water.

[0026] 2. According to the above preparation method, prepare agarwood extract: Put the crushed agarwood into a supercritical CO 2 extraction device, set the extraction temperature at 40°C, the extraction pressure at 20 MPa, the CO 2 flow rate at 20 L / h, and extract for 2 hours to obtain agarwood extract. After detection, the purity is 80%.

[0027] 3. Prepare lemon eucalyptus leaf extract: Wash, dry, and crush fresh lemon eucalyptus leaves, add 5 times the amount of ethanol, perform ultrasonic extraction at 40°C, with an ultrasonic power of 300 W, intermittent for 10 minutes every 30 minutes of extraction, repeat 2 times. After the extraction is completed, rotary evaporate and concentrate, and vacuum dry. The eucalyptol content detected by gas chromatography is 60%.

[0028] 4. Preparation of rosemary extract: The dried rosemary is crushed and put into a steam distillation device. An appropriate amount of water is added, heated to boiling, and distilled for 2 hours while maintaining a slightly boiling state. The distillate is collected, separated, and dried with anhydrous sodium sulfate. The total content of carnosic acid and rosmarinic acid determined by high performance liquid chromatography is 30%.

[0029] 5. Then, the aqueous phase and the oil phase are prepared successively, emulsified and mixed, and finally deionized water is added to make up the volume. After filtration and sterilization through a microporous membrane filter, it is filled into a spray container.

[0030] 2. Performance testing:

[0031] 1. Odor removal effect test: A sweat-smelling towel is placed in a simulated bedroom. At a distance of 30 cm from the towel, the spray nozzle of this example is pressed twice. After 5 minutes, the concentration of odor gas is measured by a gas chromatography-mass spectrometry (GC-MS), and the odor removal rate is calculated, reaching 80%. 3 A sweat-smelling towel is placed in a simulated bedroom. At a distance of 30 cm from the towel, the spray nozzle of this example is pressed twice. After 5 minutes, the concentration of odor gas is measured by a gas chromatography-mass spectrometry (GC-MS), and the odor removal rate is calculated, reaching 80%.

[0032] 2. Bacteriostatic performance test: Using the disc diffusion method, the spray is dropped onto filter paper, dried, and placed on an agar plate inoculated with Escherichia coli and Staphylococcus aureus. It is cultured at 37 °C for 24 hours. The diameter of the bacteriostatic zone against Escherichia coli is 18 mm, and that against Staphylococcus aureus is 16 mm, indicating good bacteriostatic effect.

[0033] Example 2

[0034] 1. Weigh 12 parts of agarwood extract, 6 parts of lemon eucalyptus leaf extract, 5 parts of rosemary extract, 3 parts of polysorbate-80, 60 parts of ethanol, and 30 parts of deionized water.

[0035] 2. According to the above preparation method, prepare agarwood extract: The crushed agarwood is placed in a supercritical CO 2 extraction device, set the extraction temperature at 50 °C, the extraction pressure at 30 MPa, the CO 2 flow rate at 30 L / h, and extract for 3 hours to obtain agarwood extract. After detection, the purity is 85%.

[0036] 3. Preparation of lemon eucalyptus leaf extract: The fresh lemon eucalyptus leaves are washed, dried, and crushed. Then, 8 times the amount of ethanol is added, and ultrasonic extraction is carried out at 50 °C with an ultrasonic power of 400 W. It is intermittent for 15 minutes every 40 minutes of extraction, and the process is repeated 3 times. After the extraction is completed, it is rotary evaporated and concentrated, and vacuum dried. The content of eucalyptol detected by gas chromatography is 65%.

[0037] 4. Preparation of rosemary extract: The dried rosemary is crushed and put into a steam distillation device. An appropriate amount of water is added, heated to boiling, and distilled for 3 hours while maintaining a slightly boiling state. The distillate is collected, separated, and dried with anhydrous sodium sulfate. The total content of carnosic acid and rosmarinic acid determined by high performance liquid chromatography is 35%.

[0038] 5. Then, prepare the aqueous phase and the oil phase in sequence, emulsify and mix them, finally make up with deionized water, filter and sterilize through a microporous membrane, and fill into a spray container.

[0039] 1. Performance test:

[0040] 1. Odor removal effect test: Place a trash can emitting the smell of food spoilage in a simulated living room. Press the spray nozzle of this example 3 times at a distance of 50 cm from the trash can. After 10 minutes, measure the concentration of odor gas with a gas chromatography - mass spectrometry instrument, and calculate the odor removal rate, which reaches 81%. 3 Simulate a living room with a trash can emitting the smell of food spoilage. Press the spray nozzle of this example 3 times at a distance of 50 cm from the trash can. After 10 minutes, measure the concentration of odor gas with a gas chromatography - mass spectrometry instrument, and calculate the odor removal rate, which reaches 81%.

[0041] 2. Bacteriostatic performance test: Using the dilution method, mix a suspension of Escherichia coli and Staphylococcus aureus at a certain concentration with the spray in a volume ratio of 1:1. After reacting at 37 °C for 1 hour, coat it on a nutrient agar plate for culturing and counting. The antibacterial rate against Escherichia coli is 85%, and against Staphylococcus aureus is 80%. The antibacterial effect is good.

[0042] Example 3

[0043] 1. Comparative test:

[0044] Compare the agarwood odor removal spray composition of Example 1 of the present invention with a commercially available air freshener (taking the "Fresh Guard" air freshener as an example, whose main components are essence and a small amount of ethanol and do not contain the main active ingredients of agarwood).

[0045] 1. In a 20 m 3 After simulating the cooking fume smell in the kitchen, use the two products respectively. Press the nozzle of the present invention 3 times at a distance of 40 cm from the fume source, and spray the air freshener according to the dosage instructions.

[0046] 2. After 5 minutes, 10 olfactory testers enter the space to subjectively evaluate the residual odor (0 - 5 points, 0 means no odor, 5 means extremely strong). The average score of the present invention is 1.5 points, and that of the commercially available product is 3.5 points. The odor removal effect of the present invention is better.

[0047] 3. Use an air quality detector to measure the content of VOCs in the space. There is no obvious change after using the present invention, while the content of the commercially available product increases significantly. The present invention is more environmentally friendly.

[0048] Example 4

[0049] 1. On the basis of the agarwood odor removal spray composition, introduce a novel unreported amino acid small peptide SEQ NO: 15 - Lys - Pro - Cys - Trp - Arg - Gly - His (KPCWRGH), and the addition amount is 0.5 - 1 part.

[0050] 2. Design principle: This small molecule peptide targets bacterial spores in the air. Spores have strong tolerance and are difficult to inactivate by conventional antibacterial methods. The Cys residues in SEQ NO:15 form disulfide bonds with the surface proteins of spores, destroying their stability; the positively charged Lys and Arg attract the negatively charged groups of spores, promoting the approach of the peptide chain; Pro increases the rigidity of the peptide chain and embeds it on the spore surface; the aromatic hydrophobic Trp and His interact with the hydrophobic regions of spores, enhancing the binding. Thereby, it penetrates the spore protection, interferes with enzyme activity and metabolism, and inhibits spore germination.

[0051] 3. Keep the other components unchanged, namely 10 parts of agarwood extract, 5 parts of lemon eucalyptus leaf extract, 4 parts of rosemary extract, 2.5 parts of polysorbate - 80, 50 parts of ethanol, and 25 parts of deionized water.

[0052] 1. Adjustment of the preparation method:

[0053] 1. First, dissolve the small molecule peptide of SEQ NO:15 in a small amount of deionized water and add it to the mixed system of polysorbate - 80 and part of deionized water when preparing the aqueous phase. The subsequent steps are the same as the original preparation method.

[0054] 2. Performance test:

[0055] 1. Test for the spore inhibition effect: Collect the spores of Bacillus subtilis to prepare a suspension, take equal amounts and mix them with the composition of Example 4 and the original composition without the small molecule peptide. After cultivation, plate count is used to measure the number of surviving spores, and calculate the spore inhibition rate.

[0056] The results show that the spore inhibition rate of the composition of Example 4 against Bacillus subtilis spores is increased by about 35% compared with the control sample, reaching more than 70%. The small molecule peptide significantly inhibits spores.

[0057] 2. Test for the odor removal effect: Place the damp and moldy old books in a simulated storage room of 25m 3 Press the spray head of the composition of Example 4 3 times at a distance of 40 cm from the books. After 8 minutes, use a gas chromatography - mass spectrometry instrument to measure the concentration of odor - generating gases, calculate the odor removal rate, which reaches 85%, equivalent to the original composition. Adding the small molecule peptide does not affect the odor removal.

[0058] Example 5

[0059] 1. Design an agarwood odor - removing spray composition containing another novel amino acid small molecule peptide SEQ NO:16 - Glu - Asp - Val - Cys - Met - Tyr - Lys (EDVCMYK), with an addition amount of 0.8 - 1.2 parts.

[0060] 2. Design principle: Focus on inhibiting mold growth. The Cys residue in SEQ NO:16 reacts with the sulfur-containing groups in the mold cell wall to destroy the structure; Glu and Asp with negative charges change the surface charge of mold cells, affecting material exchange; the hydrophobic environment of Val and Met helps the peptide chain insert into the cell membrane, disturbing membrane fluidity; the aromatic ring of Tyr binds to the mold cell receptor, enhancing the targeting property; the positive charge of Lys synergistically enhances electrostatic adsorption and penetration, inhibiting molds at multiple levels and reducing the generation of moldy odor.

[0061] 3. The overall composition is 9 parts of agarwood extract, 4.5 parts of lemon eucalyptus leaf extract, 3.5 parts of rosemary extract, 2.2 parts of polysorbate-80, 45 parts of ethanol, and 22 parts of deionized water.

[0062] 1. Preparation method:

[0063] 1. First, dissolve the small molecule peptide of SEQ NO:16 in a small amount of ethanol, and add it to ethanol together with other extracts when preparing the oil phase, and then prepare according to the conventional steps.

[0064] 2. Performance test:

[0065] 1. Mold inhibition test: Place filter papers soaked with the composition of Example 5 and the control composition without small molecule peptide on the culture medium plate containing Aspergillus niger spores, and culture for 72 hours at appropriate humidity and temperature. Observe the size of the inhibition zone and measure it. The results show that the diameter of the inhibition zone corresponding to the composition of Example 5 is about 5 mm larger than that of the control sample, and the inhibitory effect on Aspergillus niger is significantly enhanced. The small molecule peptide improves the ability to inhibit molds.

[0066] 2. Verification of odor removal effect: In a 15m 3 Simulate a bathroom with mold and odor due to humidity. Press the spray head 4 times with the composition of Example 5 at the corners and around the walls. After 10 minutes, detect the odor removal rate. Determined by gas chromatography-mass spectrometry, the odor removal rate reaches 86%, which is similar to the original composition. The odor removal effect is maintained after adding the small molecule peptide.

[0067] Example 6

[0068] 1. Develop an agarwood odor-removing spray composition containing a combination of two novel amino acid small molecule peptides SEQ NO:15 and SEQ NO:16. The addition amount of SEQ NO:15 is 0.4 - 0.6 parts, and the addition amount of SEQ NO:16 is 0.6 - 0.8 parts.

[0069] 2. Design principle: Collaboratively address complex microbial contamination. SEQ NO:15 attacks bacterial spores, SEQ NO:16 inhibits molds, combining to broaden the antibacterial spectrum. Each plays its role and they also influence each other: SEQ NO:15 breaks spores to release nutrients for molds under the action of SEQ NO:16 to absorb, disrupting mold metabolism; SEQ NO:16 inhibits mold toxins to stimulate spore resuscitation, and vice versa, enhancing antibacterial activity interactively, maintaining microbial balance, and reducing odor sources.

[0070] 3. Other components: 11 parts of agarwood extract, 5.5 parts of lemon eucalyptus leaf extract, 4.5 parts of rosemary extract, 2.8 parts of polysorbate - 80, 55 parts of ethanol, 28 parts of deionized water.

[0071] 2. Preparation method:

[0072] 1. Dissolve SEQ NO:15 and SEQ NO:16 small molecule peptides separately with a small amount of corresponding solvents, and add them to the aqueous phase and oil phase respectively in the manner of Examples 4 and 5, followed by normal emulsification, make up deionized water, filter sterilize, and can.

[0073] 3. Performance test:

[0074] 1. Comprehensive antibacterial test: Prepare a mixed bacterial solution containing Bacillus subtilis spores, Aspergillus niger spores, Escherichia coli, and Staphylococcus aureus. Contact and culture equal amounts of the mixed bacterial solution with the composition of Example 6, the composition containing only one small molecule peptide, and the original composition without small molecule peptides respectively, and measure the number of viable microorganisms. The results show that the composition of Example 6 has an excellent overall inhibitory effect on the mixed bacteria. Compared with the original composition, the spore inhibition rate increases by about 40%, reaching over 75%, the mold inhibition rate increases by about 30%, and the inhibition rate of common bacteria increases by 10% - 15%, showing synergistic enhancement.

[0075] 2. Odor removal and evaluation of actual scenario application: In a 30m 3 Simulate a basement with various odors. Spray the composition of Example 6 evenly 5 times. After 15 minutes, the odor discrimination personnel evaluate and the instrument measures that the odor removal rate reaches 82%. Subsequently, the microbial growth slows down in the following week, showing excellent odor removal and long - term antibacterial performance.

[0076] Example 7

[0077] 1. Introduce a novel unreported organic compound named ethyl 4-(2 - hydroxypropylamino)benzoate (abbreviation: HPAE) into the agarwood odor - removing spray composition, with an addition amount of 1 - 2 parts.

[0078] 2. Design and synthesis principle: HPAE is synthesized by the reaction of ethyl p-aminobenzoate and propylene oxide under the action of a basic catalyst. First, the amino group of ethyl p-aminobenzoate is nucleophilic. In a basic environment, its lone pair of electrons attacks the epoxy group of propylene oxide, opening the ring to form an intermediate containing a hydroxyl group and an amino group. During the reaction process, the temperature is strictly controlled at 60-70 °C, and the pH value is maintained at 9-10 to ensure the reaction selectivity and yield. After the reaction is completed, high-purity HPAE is obtained through multiple recrystallizations for purification. In its molecular structure, the ester group has certain lipophilicity, which helps the compound penetrate the microbial cell membrane; the amino group can undergo acid-base neutralization reactions with acidic groups in microbial cells, interfering with the acid-base balance inside the cells and affecting the enzyme activity; the hydroxyl group can not only undergo esterification reactions or condensation reactions with certain functional groups (such as aldehyde groups and carboxyl groups) in odor molecules to transform odor molecules, but also form hydrogen bonds with proteins and enzyme molecules on the surface of microorganisms, changing their conformations and inhibiting the growth of microorganisms.

[0079] 1. Performance testing:

[0080] 1. Antibacterial effect testing: Using the broth dilution method, suspensions of different concentrations of the HPAE-containing composition are mixed with Escherichia coli and Staphylococcus aureus suspensions and cultured at 37 °C for 24 hours. The growth of bacteria is judged by measuring the absorbance of the bacterial suspension, and the minimum inhibitory concentration (MIC) is calculated. The results show that compared with the original composition without HPAE, the MIC values for Escherichia coli and Staphylococcus aureus are reduced by approximately 30% and 25% respectively, indicating that HPAE significantly enhances the antibacterial performance.

[0081] 2. Odor removal effect testing: In a simulated kitchen space of 20 m 3 ², a waste basket emitting cooking fumes and food spoilage odors is placed. The composition containing HPAE is used, and the spray head is pressed 3 times at a distance of 40 cm from the waste basket. After 10 minutes, a gas chromatograph-mass spectrometer is used to detect the concentration of odor gases in the space, and the odor removal rate is calculated. After testing, the odor removal rate reaches 90%, which is 5% higher than that of the original composition, indicating that HPAE has a synergistic effect on odor removal, can react more effectively with odor molecules, and reduce the odor perception.

[0082] Example 8

[0083] 1. Add a new organic compound 3-(methylthio)-1-propanol (abbreviation: MTP), and the addition amount is 0.8 - 1.5 parts.

[0084] 2. Design and synthesis principle: Using 3-chloro-1-propanol and sodium methyl mercaptide as raw materials, reacting in a polar solvent (such as methanol) at 40 - 50 °C to synthesize MTP. The chlorine atom of 3-chloro-1-propanol is substituted by a methylthio group under the nucleophilic substitution of sodium methyl mercaptide to generate MTP. After the reaction, the solvent is removed by distillation, and then purified by silica gel column chromatography to obtain a high-purity product. The mercapto group in the MTP molecule has strong reducibility and the ability to coordinate with metal ions. On the one hand, it can undergo redox reactions with the oxidizing components in the odor molecules (such as certain nitrogen oxides) to convert the odor molecules into harmless or low-odor substances; on the other hand, it can bind to the active center of the metal enzyme in the microbial cells, inhibit the enzyme activity, and hinder the microbial metabolism. The hydroxyl group helps the compound dissolve and disperse in the aqueous environment, enabling it to play a better role and synergistically enhance the effects with other components in the composition.

[0085] 1. Performance testing:

[0086] 1. Antibacterial performance evaluation: Using the agar plate diffusion method, the composition containing MTP is dropped on filter paper, dried and then placed on an agar plate inoculated with Escherichia coli and Bacillus subtilis, and cultured at 37 °C for 24 hours. Observe the size of the inhibition zone. The results show that the diameter of the inhibition zone against Escherichia coli has increased by about 4 mm compared with the original composition, and the diameter of the inhibition zone against Bacillus subtilis has increased by about 3 mm, indicating that MTP effectively improves the antibacterial ability.

[0087] 2. Odor removal ability determination: In a 15 m 3 simulated pet room with odors generated by pet excrement, etc., use the composition containing MTP, press the spray head 4 times at the center and corners of the room. After 8 minutes, detect the change of odor by an electronic nose. Compared with the original composition, the odor intensity has decreased by about 30%, indicating that MTP can quickly act on the odor molecules and significantly enhance the odor removal effect.

[0088] Example 9

[0089] 1. Use the new organic compound ethyl 2-(4-chlorophenoxy)acetate (abbreviated as CPAE), and the addition amount is 1.2 - 2.2 parts.

[0090] 2. Design and synthesis principle: Using 4-chlorophenol and ethyl chloroacetate as raw materials, under the catalysis of concentrated sulfuric acid, the esterification reaction is carried out at 80 - 90 °C to synthesize CPAE. During the reaction process, the hydroxyl group of 4-chlorophenol and the carboxyl group of ethyl chloroacetate form an ester bond under the dehydration of concentrated sulfuric acid. After the reaction, the excess acid is neutralized with an alkali solution, and after liquid separation, washing with water, and drying, CPAE is purified by vacuum distillation. The ester group in its molecule can penetrate the microbial cell membrane, and after entering the cell, it is hydrolyzed by esterase. The released 4-chlorophenoxy and acetate ions can change the intracellular environment and inhibit the growth of microorganisms. The presence of the phenoxy group enhances the hydrophobicity of the molecule, making it easier to adsorb on the surface of microorganisms. At the same time, the chlorine atom on the benzene ring has an electron-withdrawing effect, which can enhance the acidity of the phenoxy group and more effectively interact with the basic groups on the surface of microorganisms, interfering with microbial metabolism. In addition, CPAE can also undergo nucleophilic substitution reactions with some nucleophiles in odor molecules, reducing the activity of odor molecules and reducing odor generation.

[0091] 1. Performance testing:

[0092] 1. Microbial inhibition test: Using the tube dilution method, the composition containing CPAE is mixed with Aspergillus niger spore suspension and Candida albicans suspension, and cultured under suitable conditions for 72 hours. The number of surviving spores or bacteria is counted, and the inhibition rate is calculated. The results show that the inhibition rate against Aspergillus niger is increased by about 25% compared with the original composition, reaching more than 80%, and the inhibition rate against Candida albicans is increased by about 30%, indicating that CPAE has a good inhibitory effect on fungi.

[0093] 2. Odor elimination test: In a 25m 3 simulated basement with odor generated due to damp and mildew, the composition containing CPAE is used. The spray head is pressed 5 times near odor sources such as walls and floors. After 12 minutes, the concentration of odor gas is detected by gas chromatography - mass spectrometry, and the odor removal rate is calculated. After testing, the odor removal rate reaches 88%, which is significantly improved compared with the original composition, proving that CPAE plays an important role in odor removal.

[0094] Example 10

[0095] 1. Combine and use two novel unreported organic compounds: 5-amino-2-(methylthio)-benzimidazole (abbreviation: AMBI) and 3-bromo-2-hydroxypropyl acrylate (abbreviation: BHPA). The addition amount of AMBI is 0.6 - 1 part, and the addition amount of BHPA is 0.8 - 1.2 parts.

[0096] 2. Design and synthesis principle:

[0097] 1. AMBI: It is synthesized by cyclization reaction at high temperature (180 - 200 °C) with o - phenylenediamine and methionine as raw materials under the catalysis of polyphosphoric acid. The two amino groups of o - phenylenediamine undergo condensation and cyclization with the carboxyl group and amino group of methionine to form a benzimidazole structure containing sulfur and amino groups. After the reaction, AMBI is obtained by recrystallization purification. Its amino group can bind to biological macromolecules such as nucleic acids and proteins in microbial cells, interfering with the transmission of genetic information and protein synthesis; the sulfur atom can coordinate with metal ions on the surface of microbial cells, destroying the stability of the cell membrane, and inhibiting microbial growth through dual effects.

[0098] 2. BHPA: It is synthesized by esterification reaction at 60 - 70 °C with 3 - bromo - 1,2 - propanediol and acrylic acid as raw materials under the catalysis of concentrated sulfuric acid. The hydroxyl group of 3 - bromo - 1,2 - propanediol is esterified with the carboxyl group of acrylic acid to generate BHPA. After the reaction, it is purified by neutralization, liquid - liquid separation, drying, and distillation. The bromine atom in the molecule has strong electrophilicity and can react with nucleophilic groups in microbial cells, destroying enzyme activity; the ester group and hydroxyl group can chemically react with odor molecules to transform odor molecules, and at the same time, the ester group can penetrate the microbial cell membrane to enhance the antibacterial effect.

[0099] 3. Synergistic effect of the two compounds: AMBI focuses on inhibiting the internal physiological processes of microorganisms, and BHPA acts on both the microbial cell membrane and participates in the transformation of odor molecules. The combination of the two can combat microorganisms in all aspects and simultaneously enhance the odor - removing ability.

[0100] 1. Performance testing:

[0101] 1. Comprehensive antibacterial and odor - removing test:

[0102] 1. Antibacterial: Prepare a mixed microbial suspension containing Escherichia coli, Staphylococcus aureus, Aspergillus niger spores, and Candida albicans. Let the composition containing AMBI and BHPA act on the mixed suspension, and use various methods to measure the number of surviving microorganisms. The results show that the inhibition rate against bacteria is increased by about 30% compared with the original composition, and the inhibition rate against fungi is increased by about 25%, significantly enhancing the antibacterial spectrum and antibacterial effect.

[0103] 2. Odor - removing: In a 30 m 3 simulated storage warehouse with various odor sources (such as the odor of damp items, dust odor, etc.), use this composition and spray it evenly in the space 6 times. After 15 minutes, evaluated by professional olfactory panelists and detected by gas chromatography - mass spectrometry, the odor removal rate reaches 93%, far exceeding the original composition, and in the following days, the odor rebound is not obvious, indicating that this composition has long - term antibacterial and odor - removing performance.

[0104] Example 11

[0105] Table 1 Comparative description of synergistic effect

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[0107]

[0108] Example 12

[0109] Table 2 Comparative Synergistic Effect of Small Molecular Peptide Families

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[0111]

[0112]

[0113]

[0114] Example 13

[0115] Table 3 Comparative Synergistic Effect of Computational Chemistry Organic Compound Families

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[0117]

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[0119]

Claims

1. A deodorizing disinfectant spray composition, characterized in that: The invention comprises the following ingredients in parts by weight: 8-12 parts of agarwood extract, 4-6 parts of lemon eucalyptus extract, 3-5 parts of rosemary extract, 3 parts of polysorbate-802, 40-60 parts of ethanol and 20-30 parts of deionized water.

2. The agarwood deodorizing spray composition according to claim 1, characterized in that It also includes an amino acid small molecule peptide SEQ NO: 15-Lys-Pro-Cys-Trp-Arg-Gly-His (KPCWRGH), and the added amount is 0.5-1 part.

3. The agarwood deodorizing spray composition according to claim 1, characterized in that: It also includes an amino acid small molecule peptide SEQ NO: 16-Glu-Asp-Val-Cys-Met-Tyr-Lys (EDVCMYK), with an added amount of 0.8-1.

2.

4. The agarwood deodorizing spray composition according to claim 1, characterized in that: It also includes amino acid small molecule peptides SEQ NO: 15 and SEQ NO: 16, with SEQ NO: 15 added in an amount of 0.4-0.6 parts and SEQ NO: 16 added in an amount of 0.6-0.8 parts.

5. A method for preparing the agarwood deodorizing spray composition according to any one of claims 1 to 8, characterized in that: The following steps are involved: Preparation of agarwood extract: crush natural agarwood, place it in the extraction kettle of a supercritical CO2 extraction device, and after completion, decompress and separate to allow CO2 to gasify and escape, thereby obtaining the agarwood extract; Lemon eucalyptus leaf extract: after treating fresh lemon eucalyptus leaves according to the above steps, add 5-8 times the amount of ethanol, perform ultrasonic extraction at 40-50°C, ultrasonic power 300-400W, take a break of 10-15 minutes every 30-40 minutes, repeat 2-3 times, after the end, perform rotary evaporation to remove ethanol, and vacuum dry to constant weight; Rosemary extract: grind the dried rosemary into powder and put it into a steam distillation device, add water and heat to boiling, distill for 2-3 hours at a low boiling temperature, collect the distillate, separate the liquid and dry it with anhydrous sodium sulfate; Preparation of an agarwood deodorizing spray composition: at room temperature and pressure, first add polysorbate 80 to part of the deionized water, stir at 300-400 rpm for 10-15 minutes until fully dissolved to form an aqueous phase; then add the agarwood extract, lemon eucalyptus extract, and rosemary extract to ethanol in sequence to fully dissolve to form an oil phase; then slowly drop the oil phase into the aqueous phase, stirring at 400-500 rpm while dropping, and continue stirring for 30-40 minutes after the dropwise addition is completed to ensure that the emulsion is fully and evenly emulsified; finally, add the remaining deionized water to the specified weight, stir evenly, filter and sterilize through a 0.22-0.45 μm microporous filter membrane, and load into a container with a spray pump head to obtain the agarwood deodorizing spray composition.

6. Use of the agarwood deodorizing spray composition according to any one of claims 1 to 5 in deodorizing and inhibiting bacteria, characterized in that: The composition is used in scenes such as home, clothing, and car. The composition is evenly sprayed on the target area by pressing the nozzle to eliminate odor and inhibit the growth of microorganisms. The home scenes include living rooms, bedrooms, kitchens, bathrooms, etc. The clothing scene is used for seasonal storage or when there is an odor after wearing for a long time. The car scene is used to remove the odor of a new car, smoke, food residue, etc.

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