Chilades pandava attractant composition and application

Abstract

The invention discloses a purple gray butterfly attractant composition and application, and active substances in the purple gray butterfly attractant are composed of 4, 6-dimethyldodecane, glutaric acid (2, 4-di-tert-butylphenyl) monoester, tetracosane, eicosane, pristane, octadecane, iododecane, 2, 2, 4, 4, 6, 8, 8-heptamethylnonane and phytane. The active substance is taken as an effective component, and n-hexane is taken as a diluent for combination, so that an attraction preparation can be further obtained, and the preparation has a remarkable attraction effect on adults of the chilades pandava, is small in dosage, simple and convenient to use and high in target property, can be simultaneously applied to prevention and control of the chilades pandava and dynamic population monitoring, and has a wide application prospect. The method is of great significance to research and development of a new green prevention and control technology of the chilades pandava.

Description

Technical Field

[0001] The invention belongs to the technical field of biological control, and specifically relates to a zigzag butterfly attractant composition and application. Background Art

[0002] The Lycaenidae (Lepidoptera: Lycaenidae) is a relatively small group of butterfly insects. More than 6,700 species have been recorded worldwide, and more than 600 species have been recorded in China. Most species of Lycaenidae have strong regional characteristics and are sensitive to changes in the surrounding environment. Therefore, they are often used as indicator species for ecological environment monitoring. However, some species are important pests in agriculture and forestry. For example, Chiladespandava is an important leaf-eating pest on Cycas spp. The larvae swarm to harm the tender branches, young leaves, stem tip tissues and cones of Cycas. The young larvae eat the tender leaves and only the epidermis is left. As the age of the insect increases, the leaves are eaten to form notches or even bite off, and the tender pinnae are eaten up, leaving only dry petioles. In severe cases, the Cycas plant may die. However, with the acceleration of urbanization in my country, cycads are widely cultivated as the main greening tree species in urban forestry, resulting in the occurrence and damage of the curved purple butterfly becoming increasingly serious, and becoming the dominant pest species of cycads. Under natural conditions, the natural enemies of this insect have weak control ability; the common light trapping in physical control is ineffective against it; chemical control is prone to cause environmental pollution and accidental injury to natural enemies. Therefore, it is urgent to explore a green, healthy and sustainable control technology. Summary of the invention

[0003] The purpose of the present invention is to provide a composition and application of an attractant for the serpentine butterfly, so as to provide technical support for the green prevention and control of the insect.

[0004] To achieve the above object, the present invention provides the following technical solutions:

[0005] A zigzag purple butterfly attractant composition, whose active substances consist of 4,6-dimethyldodecane, glutaric acid (2,4-di-tert-butylphenyl) monoester, tetracosane, eicosane, pristane and octadecane. Specifically, the active substances consist of 0.01 μg / μL 4,6-dimethyldodecane, 100 μg / μL glutaric acid (2,4-di-tert-butylphenyl) monoester, 100 μg / μL tetracosane, 0.01 μg / μL eicosane, 100 μg / μL pristane and 10 μg / μL octadecane. The above-mentioned zigzag purple butterfly attractant composition is made into a zigzag purple butterfly female insect attractant.

[0006] A zigzag purple butterfly attractant composition, the active substances are composed of 4,6-dimethyldodecane, glutaric acid (2,4-di-tert-butylphenyl) monoester, tetracosane, eicosane, pristane, octadecane, and iododecane. Specifically, the active substances are composed of 0.01μg / μL 4,6-dimethyldodecane, 100μg / μL glutaric acid (2,4-di-tert-butylphenyl) monoester, 100μg / μL tetracosane, 0.01μg / μL eicosane, 100μg / μL pristane, 10μg / μL octadecane, and 0.1μg / μL iododecane. The above-mentioned zigzag purple butterfly attractant composition is made into a zigzag purple butterfly male attractant.

[0007] A zigzag purple butterfly attractant composition, the active substance is composed of 4,6-dimethyldodecane, glutaric acid (2,4-di-tert-butylphenyl) monoester, tetracosane, eicosane, pristane, octadecane, iododecane, 2,2,4,4,6,8,8-heptamethylnonane and phytane. Specifically, the active substance is composed of 0.01μg / μL 4,6-dimethyldodecane, 100μg / μL glutaric acid (2,4-di-tert-butylphenyl) monoester, 100μg / μL tetracosane, 0.01μg / μL eicosane, 100μg / μL pristane, 10μg / μL octadecane, 0.1μg / μL iododecane, 100μg / μL 2,2,4,4,6,8,8-heptamethylnonane and 100μg / μL phytane. The above-mentioned Zigzag Butterfly attractant composition is made into Zigzag Butterfly attractant.

[0008] The preparation method of the aforementioned attractant for the purple butterfly (female and male) is as follows: the active substance is diluted with n-hexane to a certain concentration, and a certain volume (10 μL) is drawn according to the dosage and added to the attractant core. The prepared attractant core is stored in a refrigerator at -20°C for future use.

[0009] The present invention also claims to protect the application of the attractant composition for Curved Lithops spheniscus in the prevention and control of Curved Lithops spheniscus and population monitoring.

[0010] The present invention has the following beneficial effects:

[0011] It is proved by gas chromatography-electroantennary coupling technology (GC-EAD) and behavioral experiments that the active substance of the present invention can lure adults of the butterfly, has a strong trapping effect on the adults of the butterfly, is environmentally friendly, harmless to the human body, can be accurately applied to the prediction of the insect situation and trapping and prevention of the butterfly, and has good economic and ecological benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1The GC-EAD reactions of the male and female adults of the present invention to the extracts from the adult body surface are shown in Figure 1. A, the GC-EAD reaction of the male insect to the whole body extract of the female insect; B, the GC-EAD reaction of the male insect to the extract of the female insect's forewing; C, the GC-EAD reaction of the male insect to the extract of the female insect's hindwing; D, the GC-EAD reaction of the female insect to the whole body extract of the male insect; E, the GC-EAD reaction of the female insect to the extract of the male insect's forewing; F, the GC-EAD reaction of the female insect to the extract of the male insect's hindwing.

[0013] Figure 2 The antennal potential response of male and female adults of the present invention's embodiment of the purple butterfly to a single active substance in the body surface extract. A. 4,6-dimethyldodecane, B. glutaric acid (2,4-di-tert-butylphenyl) monoester, C. iododecane, D. tetracosane, E. eicosane, F. pristane, G. 2,2,4,4,6,8,8-heptamethylnonane, H. octadecane, I. phytane; the data in the figure are mean ± standard error. Different uppercase and lowercase letters indicate significant differences at the 0.05 level (Tukey's HSD, P<0.05), and "*" indicates significant differences between males and females at the 0.05 level (independent sample t test, P<0.05).

[0014] Figure 3 This is the directional behavioral response of male and female adults of the butterfly to a single active substance in the body surface extract of the embodiment of the present invention. A1: 0.01 μg / μL 4,6-dimethyldodecane, A2: 100 μg / μL glutaric acid (2,4-di-tert-butylphenyl) monoester, A3: 0.1 μg / μL iodododecane, A4: 100 μg / μL tetracosane, A5: 0.01 μg / μL eicosane, A6: 100 μg / μL pristane, A7: 100 μg / μL 2,2,4,4,6,8,8-heptamethylnonane, A8: 10 μg / μL octadecane, A9: 100 μg / μL phytane, A10: n-hexane; Data in the figure are mean ± standard error, different uppercase and lowercase letters on the error bars indicate significant differences at the 0.05 level (Kruskal-Wallis test), and "*" indicates significant differences between males and females at the 0.05 level (Mann-Whitney test).

[0015] Figure 4 The directional behavior response of male and female adults of the butterfly to the mixture of body surface extracts in the embodiment of the present invention. The data in the figure are mean ± standard error, and different uppercase and lowercase letters on the error bar indicate significant differences at the 0.05 level (Kruskal-Wallis test), and "*" indicates significant differences between males and females at the 0.05 level (Mann-Whitney test). DETAILED DESCRIPTION

[0016] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings and specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0017] Example 1

[0018] Collection, analysis and determination of active substances from the body surface extracts of male and female adults of the butterfly

[0019] Collection of active substances from the body surface extracts of male and female adults of the butterfly: The body surface extracts of one-day-old adults of the butterfly were extracted by solvent extraction. At the peak of mate seeking, healthy male and female adults of the butterfly were soaked in 2 mL of n-hexane solution for 30 minutes to extract the whole body volatiles of male and female adults; the forewings and hindwings of male and female adults were quickly cut off with dissecting scissors, and soaked in 2 mL of n-hexane solution for 30 minutes to extract the volatiles of the forewings and hindwings. The soaked liquid was filtered through a 0.22 μm organic filter membrane and concentrated to 100 μL with nitrogen. Then, the extract was filled in a new brown injection bottle with a pointed bottom glass tube, sealed, and stored at -20°C for use.

[0020] GC-MS detection conditions: the chromatographic column was a DB-5ms Ultra Inert capillary column (30m×250μm×0.25μm), the carrier gas was helium, the injection volume was 1μL, the constant flow splitless mode, and the flow rate was 1mL·min -1 Column oven heating program: starting temperature 50℃, hold for 3min, 20℃·min -1 Heating to 125℃, 10℃·min -1 The temperature was raised to 300°C and maintained for 4 minutes. The EI ion source, electron energy 70 eV, proton scanning range 50-550 amu, quadrupole temperature 150°C, ion source temperature 230°C, electron multiplier voltage 1200 V. Each component was analyzed and compared with the mass spectrum of the standard compound in the standard spectral library (NIST17.0) for qualitative analysis.

[0021] GC-EAD analysis of the extracts from the body surface of the butterfly: Use surgical scissors to quickly cut off the antennae of the insect to be tested, pick up the antennae with an insect pin, connect the two ends of the antennae to the electrodes coated with conductive glue, and place the antennae about 1 cm away from the outlet of the odor tube. The continuous airflow is 300 mL min -1The conditions of gas chromatography are the same as those of GC-MS. The outlet of the chromatographic column is connected to a splitter with a split ratio of 1:1. Part of the split compound enters the hydrogen flame ionization detector (FID), and the other part is blown to the antennae through a constant temperature heating sleeve. The GC-EAD software is used to simultaneously record the gas chromatogram and antennae potential diagram. The peak area and retention time of the compound are compared to identify the substance that produces the potential reaction in the antennae of male and female butterfly. Only one antennae are used for each male and female adult, and each antennae are tested only once, which is repeated 4 times.

[0022] like Figure 1 As shown in the figure, the body surface extracts of male and female butterfly were analyzed by GC-EAD. The results showed that there were 15 substances in which female butterfly had antennae potential response to the volatiles of male butterfly, namely 4,5-dimethylnonane, 2,2,4,4,6,8,8-heptamethylnonane, 4,6-dimethyldodecane, 3-ethyl-2,6,10-trimethylundecane, glutaric acid (2,4-di-tert-butylphenyl) monoester, 2,6,11,15-tetramethylhexadecane, phytane, octadecane, iodocosane, pristane, iodocosane, eicosane, tetracosane, tetracosylcyclododecane, and dioctyl terephthalate. Among them, dioctyl terephthalate was only found to induce antennal potential response in female insects in whole-body extracts; iodohexadecane was only found to induce antennal potential response in female insects in hind-wing extracts; eicosane, phytane, 2,6,11,15-tetramethylhexadecane, pristane and tetramethylcyclododecane were only found to induce antennal potential response in female insects in whole-body and hind-wing extracts.

[0023] There are 10 substances that males of the butterfly have electroantennae responses to volatiles from females, namely 4,5-dimethylnonane, 2,2,4,4,6,8,8-heptamethylnonane, 4,6-dimethyldodecane, 3-ethyl-2,6,10-trimethylundecane, 5-hydroxy-2,4-di-tert-butylphenyl valerate, octadecane, 2,6,11,15-tetramethylhexadecane, iododecane, phytane, and tetramethylcyclododecane. Among them, iododecane was only found in the whole body and hind wing extracts to induce electroantennae responses in males.

[0024] Example 2

[0025] Electroantennary responses of male and female adults of the butterfly Lycoris flexuosa to single active substances extracted from its body surface

[0026] Chromatographically pure n-hexane was used as the solvent, and each standard compound was diluted into five concentration gradients of 0.01μg / μL, 0.1μg / μL, 1μg / μL, 10μg / μL, and 100μg / μL. Chromatographically pure n-hexane was used as the control. Before the test, the antennae of the adult insect to be tested were completely cut off from the antennal fossa. At the same time, the base and top of the antennae were quickly cut off about 1mm under a microscope (so that the antennae can better contact the conductive gel). Then, they were fixed between two metal electrodes through conductive gel to form a closed loop. The antenna potentiometer was debugged, and the EAG activity was measured after the baseline of the measurement interface was stable. During the test, 5μL of the test solution was taken with a pipette and evenly dripped on a 0.5cm×4cm qualitative filter paper strip. After the n-hexane evaporated, it was immediately placed in the wide end of the Pasteur tube and connected to the stimulating airflow tube. The stimulating airflow was 300mL min -1 Each stimulation lasted for 1 second, and the interval between two stimulations was 60 seconds to ensure that the antennae restored their sensitivity. For the same antennae, first do a n-hexane control, then stimulate the antennae with the test compound from low concentration to high concentration, stimulate each concentration once, and finally do a n-hexane control again, repeat 5 times.

[0027]

[0028] Wherein, ST: EAG response value (mV) of the test sample with different concentration gradients; CK1: the average value (mV) of the reaction of the same test sample with different concentration gradients measured in the first time against n-hexane (CK) in this round; CK2: the average value (mV) of the reaction of the same test sample with the same concentration gradient measured in the second time against n-hexane (CK) in this round.

[0029] Statistical analysis: One-way analysis of variance (ANOVA) was used to analyze the antennal potential responses of the butterfly to different concentrations of a single active substance, and Tukey's HSD test was used for multiple comparisons. The antennal potential responses of male and female adults to the same concentration were analyzed using independent sample t-test.

[0030] like Figure 2 As shown in the figure, under the concentration gradient, the antennal potential response values ​​of male and female adults of the butterfly to different single active substances are different, as shown in the following:

[0031] The antennal potential response values ​​of male and female 4,6-dimethyldodecane of the butterfly became smaller with the increase of concentration, and were significantly higher at 0.01μg / μL than other concentrations, but there was no significant difference between the antennal potential response values ​​of females at 100μg / μL and 0.01μg / μL, and there was no significant difference between the relative values ​​of EAG response of male and female adults to 4,6-dimethyldodecane at the same concentration.

[0032] The antennal potential response values ​​of male and female Lithoptera sphenodontia to glutaric acid (2,4-di-tert-butylphenyl) monoester increased with the increase of concentration, and were significantly higher at 100 μg / μL than at other concentrations. In addition, the antennal potential response values ​​of female adults of Lithoptera sphenodontia at 100 μg / μL were significantly higher than those of males (t 8 =2.955, P<0.05);

[0033] The antennal potential response values ​​of male and female adults of the butterfly to iodine first increased and then decreased with the increase of concentration. The antennal potential response value of females to iodine reached the maximum value at 0.1μg / μL, and the antennal potential response value of males to iodine showed no significant change with the increase of concentration. There was no significant difference between the relative values ​​of EAG response of male and female adults to iodine at the same concentration.

[0034] The antennal potential response value of female adults of the butterfly to tetracosane increased with the increase of concentration, and was significantly higher at 100μg / μL than at other concentrations, but the antennal potential response value of male adults to tetracosane decreased with the increase of concentration, but there was no significant difference. In addition, the antennal potential response value of male adults of the butterfly to tetracosane decreased at 0.01μg / μL (t 8 =-2.639, P<0.05) and 0.1 μg / μL (t 8 =-3.654, P<0.05) concentration, the antennal potential response value was significantly higher than that of female insects;

[0035] The antennal potential response value of female Lithops sphenoidea to eicosane increased with the increase of concentration, but there was no significant difference. However, the antennal potential response value of male Lithops sphenoidea to eicosane decreased with the increase of concentration, which was higher at 0.01μg / μL and 0.1μg / μL, and was lower at 0.1μg / μL (t 8 =-3.293, P<0.05) concentration, the antennal potential response value was significantly higher than that of female insects;

[0036] The antennal potential response value of female P. flexuosa to pristane increased with the increase of concentration, and was significantly higher at 100 μg / μL than other concentrations. The antennal potential response value of male P. flexuosa to pristane first increased and then decreased with the increase of concentration, but there was no significant difference. There was no significant difference in the relative value of EAG response of male and female adults to pristane at the same concentration.

[0037] The antennal potential response values ​​of male and female sphenotype butterfly to 2,2,4,4,6,8,8-heptamethylnonane increased with the increase of concentration. The antennal potential response values ​​of females at 100 μg / μL were significantly higher than those at other concentrations, while there was no significant difference in males. In addition, there was no significant difference in the relative values ​​of EAG response of male and female adults to 2,2,4,4,6,8,8-heptamethylnonane at the same concentration.

[0038] The antennal potential response values ​​of male and female Lithops sphenopsus to octadecane first increased and then decreased with the increase of concentration. There was no significant difference in females, but the antennal potential response values ​​of males at 10 μg / μL were significantly higher than those at other concentrations. 8 =-2.873, P<0.05) concentration, the antennal potential response value was significantly higher than that of female insects;

[0039] The antennal potential response values ​​of both male and female Lithops sphenoidea to phytane increased with the increase of concentration. The antennal potential response values ​​of males at 100 μg / μL were significantly higher than those at other concentrations, while there was no significant difference in females. 8 =-2.637,P<0.05), 1μg / μL(t 8 =-2.509,P<0.05), 10μg / μL(t 8 =-2.931,P<0.05), 100μg / μL(t 8 =-2.595, P<0.05) concentrations were significantly higher than those of female insects.

[0040] In summary, the optimal reaction concentrations that can induce a larger antennal potential response in the butterfly are: 0.01μg / μL 4,6-dimethyldodecane, 100μg / μL glutaric acid (2,4-di-tert-butylphenyl) monoester, 0.1μg / μL iodododecane, 100μg / μL tetracosane, 0.01μg / μL eicosane, 100μg / μL pristane, 100μg / μL 2,2,4,4,6,8,8-heptamethylnonane, 10μg / μL octadecane, and 100μg / μL phytane.

[0041] Example 3

[0042] Directional behavioral responses of male and female adults of the butterfly Lycoris flexuosa to a single active substance extracted from its body surface

[0043] Using n-hexane as solvent, the single active component of the body surface extract was diluted to 0.01μg / μL 4,6-dimethyldodecane, 100μg / μL glutaric acid (2,4-di-tert-butylphenyl) monoester, 0.1μg / μL iododecane, 100μg / μL tetracosane, 0.01μg / μL eicosane, 100μg / μL pristane, 100μg / μL 2,2,4,4,6,8,8-heptamethylnonane, 10μg / μL octadecane, and 100μg / μL phytane. n-Hexane was used as a control.

[0044] Under natural light conditions, 1-day-old virgin male and female adults with intact antennae and wings and healthy and active were selected for wind tunnel behavioral response tests during the peak period of mate seeking (09:00-15:59). The indoor temperature of the wind tunnel was 25±1℃, and the experimental wind speed was set to 0.5m / s. The hook for placing the lure was 25cm long, 10cm away from the activated carbon sponge, and pasted on the top of the wind tunnel. The test used a filter paper lure, which was triangular, with a base length of 2cm and a height of 1.5cm. The release platform was 25cm high and 10cm away from the downwind screen.

[0045] First, 10 adult insects to be tested were placed in a plastic cup with many ventilation holes. After entering the peak period of mate seeking, the test insects were placed in the wind tunnel conditions for more than 30 minutes to adapt, and then the wind tunnel behavior was measured. The experiment lasted no more than 6 hours. Before the test, 10 μL of the sample solution to be tested was dripped on the filter paper. After waiting for 30 seconds for the solvent to evaporate, the filter paper lure was inserted on the hook, and then the test insects were placed on the release table with the cup facing the upwind end to start the test. The test time was 15 minutes. The behavioral responses of the test insects were observed and recorded within 15 minutes. The behavioral responses included: no response (flying against the wind less than 50 cm), directional flight (flying against the wind 50 cm-125 cm), half-way (flying against the wind more than 125 cm), and approaching the taste source (less than 30 cm from the lure). Each lure was used only once, and each group of test insects was used only once. After the observation, the test insects were caught back in the cup and moved out of the wind tunnel. After 15 minutes, the next round of testing was carried out. Before changing the type of lure, spray the inner wall of the wind tunnel with anhydrous ethanol and quickly wipe it clean to eliminate the residual influence of the volatiles of the previous lure. After the anhydrous ethanol evaporates and is discharged from the wind tunnel, put the next group of adults to be tested into the wind tunnel to adapt to the wind tunnel environment. 30 male and female adults were tested for each sample.

[0046] Statistical analysis: The behavioral responses of C. zigzag to single active components were analyzed using the Kruskal-Wallis test, and the behavioral responses of male and female adults to the same substance were analyzed using the Mann-Whitney test.

[0047] like Figure 3 As shown, the results of wind tunnel behavior tests of single components showed that all substances could induce reactions in male and female adults of the butterfly, but the degree of reaction caused by different compounds was different.

[0048] like Figure 3 -A shows that all compounds can cause adults to leave the release platform, but the reaction rate of male and female adults of the butterfly to n-hexane is significantly higher than that of other substances in the non-reaction stage (female: H = 24.866, P < 0.01; male: H = 17.500, P < 0.05), indicating that n-hexane has the worst effect on attracting them. In addition, the probability of female butterfly not responding to iododecane is significantly higher than that of male butterfly (Z = 2.023, P < 0.05).

[0049] like Figure 3 -B, during the directional flight stage, the reaction rate of females to pristane was significantly higher than that of other substances (H=19.939, P<0.05), the reaction rate of males to iododecane was significantly higher than that of other substances (H=20.990, P<0.05), and the reaction rate of males to iododecane (Z=-2.023, P<0.05) and 2,2,4,4,6,8,8-heptamethylnonane (Z=-2.023, P<0.05) was significantly higher than that of females.

[0050] like Figure 3 -C, in the half-stage, the reaction rate of female insects to tetracosane and 2,2,4,4,6,8,8-heptamethylnonane was significantly higher than that of other substances (H=19.742, P<0.05), the reaction rate of male insects to eicosane was significantly higher than that of other substances (H=19.536, P<0.05), and there was no significant difference between male and female adults to the same substance.

[0051] like Figure 3 -D shows that in the approach stage, the reaction rate of female insects to eicosane was significantly higher than that of other substances (H=21.920, P<0.01), the reaction rate of male insects to tetracosane was significantly higher than that of other substances (H=21.174, P<0.05), and the reaction rate of female insects to eicosane was significantly higher than that of male insects (Z=-2.023, P<0.05).

[0052] In summary, the behavioral response rates of female insects to different single active substances are from high to low: eicosane>2,2,4,4,6,8,8-heptamethylnonane>tetracosane>octadecane>4,6-dimethyldodecane>glutaric acid (2,4-di-tert-butylphenyl) monoester>phytane>iododecane>n-hexane>pristane. The response rates of male insects to different single substances are from high to low: tetracosane>eicosane>octadecane>2,2,4,4,6,8,8-heptamethylnonane>iododecane>phytane>pristane>glutaric acid (2,4-di-tert-butylphenyl) monoester>4,6-dimethyldodecane>n-hexane.

[0053] Example 4

[0054] Orientational behavioral responses of male and female adults of the butterfly Lycoris flexuosa to a mixture of body surface extracts

[0055] In order to screen out the mixed formula with better attracting effect on the butterfly and the key components for attraction, different multi-component formulas were designed for wind tunnel tests. The M1 formula is a mixture of all the components screened out from the body surface extracts at the optimal concentrations of each antennal potential in equal proportions. The components in the M1 formula are gradually reduced by one or more according to the sequence to form the M2-M10 formulas. If a certain component does not weaken the attracting effect of the original formula on the butterfly after being removed from the original mixed component formula, it means that this component has little effect on the butterfly; if the removed component weakens the attracting effect of the original formula, it means that this component is necessary and is the key component of attraction. The specific formula is as follows: Figure 4 shown.

[0056] The method for preparing a mixture of extracts from the body surface of adult butterfly Lycaenidae includes:

[0057] Ratio M1: 0.01μg / μL 4,6-dimethyldodecane + 100μg / μL glutaric acid (2,4-di-tert-butylphenyl) monoester + 0.1μg / μL iodododecane + 100μg / μL tetracosane + 0.01μg / μL eicosane + 100μg / μL pristane + 100μg / μL 2,2,4,4,6,8,8-heptamethylnonane + 10μg / μL octadecane + 100μg / μL phytane.

[0058] Ratio M2: 0.01μg / μL 4,6-dimethyldodecane + 100μg / μL glutaric acid (2,4-di-tert-butylphenyl) monoester + 0.1μg / μL iododecane + 100μg / μL tetracosane + 0.01μg / μL eicosane + 100μg / μL pristane + 100μg / μL 2,2,4,4,6,8,8-heptamethylnonane + 10μg / μL octadecane

[0059] Ratio M3: 0.01μg / μL 4,6-dimethyldodecane + 100μg / μL glutaric acid (2,4-di-tert-butylphenyl) monoester + 0.1μg / μL iododecane + 100μg / μL tetracosane + 0.01μg / μL eicosane + 100μg / μL pristane + 100μg / μL 2,2,4,4,6,8,8-heptamethylnonane

[0060] Ratio M4: 0.01μg / μL 4,6-dimethyldodecane + 100μg / μL glutaric acid (2,4-di-tert-butylphenyl) monoester + 0.1μg / μL iododecane + 100μg / μL tetracosane + 0.01μg / μL eicosane + 100μg / μL pristane + 10μg / μL octadecane

[0061] Ratio M5: 0.01μg / μL 4,6-dimethyldodecane + 100μg / μL glutaric acid (2,4-di-tert-butylphenyl) monoester + 0.1μg / μL iododecane + 100μg / μL tetracosane + 0.01μg / μL eicosane + 10μg / μL octadecane

[0062] Ratio M6: 0.01μg / μL 4,6-dimethyldodecane + 100μg / μL glutaric acid (2,4-di-tert-butylphenyl) monoester + 0.1μg / μL iododecane + 100μg / μL tetracosane + 100μg / μL pristane + 10μg / μL octadecane

[0063] Ratio M7: 0.01μg / μL 4,6-dimethyldodecane + 100μg / μL glutaric acid (2,4-di-tert-butylphenyl) monoester + 0.1μg / μL iododecane + 0.01μg / μL eicosane + 100μg / μL pristane + 10μg / μL octadecane

[0064] Ratio M8: 0.01μg / μL 4,6-dimethyldodecane + 100μg / μL glutaric acid (2,4-di-tert-butylphenyl) monoester + 100μg / μL tetracosane + 0.01μg / μL eicosane + 100μg / μL pristane + 10μg / μL octadecane

[0065] Ratio M9: 0.01μg / μL 4,6-dimethyldodecane + 100μg / μL tetracosane + 0.01μg / μL eicosane + 100μg / μL pristane + 10μg / μL octadecane

[0066] Ratio M10: 100μg / μL tetracosane + 0.01μg / μL eicosane + 100μg / μL pristane + 10μg / μL octadecane

[0067] The test and analysis methods in Example 3 were used to determine the directional behavioral responses of male and female adults of the butterfly to the mixtures of the 10 ratios.

[0068] like Figure 4 As shown, all formulations can induce reactions in male and female adults of the butterfly, but the degree of reaction induced by different formulations varies to a certain extent.

[0069] like Figure 4 -A, all formulations could cause adults to leave the release platform, but there was no significant difference in the response rates of male and female adults of the butterfly to each formulation during the non-response stage (females: H = 12.769, P = 0.173; males: H = 13.203, P = 0.154).

[0070] like Figure 4-B, in the directional flight stage, the response rates of male and female adults to the M4 formula were significantly lower than those to other substances (female insects: H = 21.036, P < 0.05; male insects: H = 22.377, P < 0.01), and there was no significant difference in the response rates of male and females to the same formula.

[0071] like Figure 4 -C, in the half-stage, the reaction rate of female insects to M4 was significantly higher than that of other substances (H = 20.383, P < 0.05), and there was no significant difference in the reaction rate of male insects to each formula (H = 12.399, P = 0.192). In addition, the reaction rates of female insects to M4 (Z = -2.121, P < 0.05) and M5 (Z = -2.032, P < 0.05) were significantly higher than those of male insects.

[0072] like Figure 4 -D shows that at the approach stage, the reaction rates of male and female insects to M3, M5, M6 and M7 were significantly lower than those of other substances (female insects: H = 24.039, P < 0.01; male insects: H = 21.216, P < 0.05), indicating that the reduced octadecane, pristane, eicosane and tetracosane in these three formulas are important substances. In addition, the reaction rate of female insects to M8 was significantly higher than that of male insects (Z = -1.993, P < 0.05), and significantly higher than other formulas, indicating that M8 formula is the best formula for attracting female insects; the reaction rate of male insects to M4 was significantly higher than that of female insects (Z = -1.993, P < 0.05), and significantly higher than other formulas, indicating that M4 formula is the best formula for attracting male insects.

[0073] Example 5

[0074] The trapping effect of the mixture of body surface extracts on male and female adults of the butterfly under semi-natural conditions

[0075] Make a small hole in the center of a 0.2mm thick PVC transparent plastic plate so that a 0.5mL pointed bottom centrifuge tube with a lid can be placed in it to hold the formulated attractant. Evenly coat the entire transparent plastic plate with insect glue and hang it in a net cage (length × width × height = 2.5m × 1.5m × 3m). At this time, the transparent plastic plate can sway gently with the wind. Set up the test treatment as follows: In the directional behavior reaction test in Example 4 above, the two active ingredient mixtures M4 and M8 with the best trapping effect on male and female adults of the curved pattern purple gray butterfly were screened out for treatment, n-hexane was used as the control, and CK was a blank transparent plastic plate. Each transparent plastic plate is coated with insect glue. The specific ratio is as follows

[0076] Ratio M4: 0.01μg / μL 4,6-dimethyldodecane + 100μg / μL glutaric acid (2,4-di-tert-butylphenyl) monoester + 0.1μg / μL iododecane + 100μg / μL tetracosane + 0.01μg / μL eicosane + 100μg / μL pristane + 10μg / μL octadecane

[0077] Ratio M8: 0.01μg / μL 4,6-dimethyldodecane + 100μg / μL glutaric acid (2,4-di-tert-butylphenyl) monoester + 100μg / μL tetracosane + 0.01μg / μL eicosane + 100μg / μL pristane + 10μg / μL octadecane

[0078] The above-treated sticky insect board and the 1-day-old healthy male and female adults (20 males and 20 females) were placed in a net cage, and 100 μL of the above-mentioned formula lure was added to a 0.5 mL centrifuge tube. The trapping effect was observed every day. Only the adults that stayed on the sticky board were considered to be successfully trapped. The gender and number of adults trapped by different lures were recorded. One day was a repetition, and it was repeated 5 times. Each repetition replaced the formula lure, sticky insect board, and a new batch of 1-day-old healthy male and female adults, and the hanging position of the sticky insect board was changed clockwise (east, south, west, north, center) to eliminate the influence of position on the trapping effect.

[0079] Statistical analysis: The Kruskal-Wallis test was used to test the trapping effect of the mixture of body surface extracts on male and female adults of the butterfly under semi-natural conditions.

[0080] As shown in Table 1, under semi-natural conditions, the trapping effect of the M4 formula was the best, trapping 1 female and 3 male insects; the M8 formula trapped 2 female insects and 1 male insect; n-hexane trapped 1 female insect; and the trapping effect of the blank transparent plastic plate CK was 0.

[0081] Table 1 Different formulations of attractants in the semi-natural of the trapping results

[0082]

[0083] Note: M4 and M8 are the mixed ratios with the highest attraction rates for male and female insects screened out in the wind tunnel behavior reaction test in Example 4; CK is a blank transparent plastic plate. Different lowercase letters indicate significant differences at the 0.05 level (Kruskal-Wallis test).

Claims

1. A composition of attractant for the serpentine butterfly, characterized in that: The active substances are composed of 4,6-dimethyldodecane, glutaric acid (2,4-di-tert-butylphenyl) monoester, tetracosane, eicosane, pristane and octadecane.

2. The attractant composition of the butterfly according to claim 1, characterized in that: The active substance consists of 0.01 μg / μL 4,6-dimethyldodecane, 100 μg / μL glutaric acid (2,4-di-tert-butylphenyl) monoester, 100 μg / μL tetracosane, 0.01 μg / μL eicosane, 100 μg / μL pristane and 10 μg / μL octadecane.

3. The attractant composition of the butterfly according to claim 1, characterized in that: The active substance also includes iodododecane.

4. The attractant composition of the serpentine butterfly according to claim 2, characterized in that: The active substance also includes 0.1 μg / μL iodododecane.

5. The attractant composition for the serpentine butterfly according to claim 3, characterized in that: The active substances also include 2,2,4,4,6,8,8-heptamethylnonane and phytane.

6. The attractant composition for the serpentine butterfly according to claim 4, characterized in that: The active substances also include 100 μg / μL 2,2,4,4,6,8,8-heptamethylnonane and 100 μg / μL phytane.

7. Use of the attractant composition for Lithoptera serrulata according to claim 1 or 2 in preparing an attractant for Lithoptera serrulata females.

8. Use of the attractant composition for Lithoptera serrulata according to claim 3 or 4 in preparing an attractant for Lithoptera serrulata male insects.

9. Use of the Zigzag Lycaenidae attractant composition according to claim 5 or 6 in preparing a Zigzag Lycaenidae attractant.

10. Use of the attractant composition of any one of claims 1 to 6 for the control and population monitoring of the butterfly.

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