Sterilization composition containing bupirimate and application thereof

Abstract

The invention relates to a bupirimate-containing bactericidal composition and application thereof, the bactericidal composition comprises an active component A and an active component B, the active component A is bupirimate, and the active component B is any one of Faneptadoquin or Bifemetstrobin; the mass ratio of the active component A to the active component B is (1: 45)-(60: 1). The bactericidal composition disclosed by the invention has a synergistic effect on phytopathogen under the condition that the application amount of the active compound is reduced, the bactericidal spectrum is widened, and the generation of drug resistance is effectively retarded and avoided.

Description

Technical Field

[0001] The invention relates to the field of agricultural fungicides, and in particular to a fungicide composition containing ethynol sulfonate and a use thereof. Background Art

[0002] Powdery mildew of melons is a common fungal disease that occurs on melon crops all over my country. It is widely distributed and causes serious damage. The pathogens are mainly Sphaerotheca fiuligenea and Erysiphecichoracearum. In my country, Sphaerotheca fiuligenea is a common parasite on cucumbers. Cucumbers, pumpkins, melons, and zucchini are particularly affected. The lesions are small white, nearly circular spots at the beginning, which expand later. Most of the lesions are connected and covered with white powdery substances. The white powdery substances turn gray or gray-brown in the later stage. The incubation period of the powdery mildew pathogen of melons is short, and it can reproduce multiple generations in one growing season. It also has a high reproduction rate, which leads to a decline in crop quality and yield, seriously affecting and restricting the safe production of crops. It has become a major problem that needs to be solved urgently.

[0003] At present, chemical agents are one of the main control technologies. The fungicides used to control powdery mildew of melons mainly include triazoles, morpholines and methoxyacrylates. Bupirimate, international common name: Bupirimate, CAS number: 41483-43-6. Bupirimate is a new generation of systemic fungicide, which belongs to adenosine nucleoside deaminase inhibitor. It has strong systemic conductivity, is resistant to rain erosion, has a long lasting effect after application, and is highly effective and broad-spectrum. It has both protective and therapeutic effects, can be quickly absorbed by roots, stems, and leaves, and circulates to various parts of the plant body. It has protective and therapeutic effects, and is particularly effective against powdery mildew of crops. It is used to control powdery mildew of ornamental plants such as fruits, vegetables, and flowers, as well as field crops.

[0004] Feneptamidoquin (quinoline amine) is a quinoline carboxamide fungicide developed by Syngenta. It has a broad control spectrum, is safe and efficient, and can effectively control a variety of fungal diseases. Its CAS registration number is 2132414-06-1 (racemic 2132414-04-9). Its chemical structure is as follows:

[0005]

[0006] Bifemetstrobin (bifenthrin) is a strobilurin fungicide developed by Sumitomo Chemical Co., Ltd., CAS registration number: 2454319-63-0, chemical structure is as follows:

[0007]

[0008] In agricultural production, due to the long-term use of a single fungicide or the unscientific use of pesticides, the drug resistance of pathogenic bacteria has gradually increased; at the same time, the excessive use of pesticides has led to environmental pollution and increased production costs, which is not conducive to the sustainable development of agriculture. The applicant has conducted a large number of experimental studies and found that the compounding of ethirimol sulfate with either Bifemetstrobin or Feneptamidoquin shows good control effects on plant pathogenic bacteria, especially powdery mildew of cucurbits, within a certain range, with obvious synergistic effects, reducing the dosage of pesticides, lowering production costs, and being environmentally safe. Summary of the Invention

[0009] Based on the above situation, the object of the present invention is to provide a bactericidal composition containing ethirimol sulfate and its use. The bactericidal composition achieves a synergistic effect through compounding, effectively reducing production costs. The bactericidal composition can be used to control diseases caused by plant pathogenic bacteria, reduce the dosage of pesticides, and at the same time delay the generation and development of drug resistance of pathogenic bacteria, being environmentally friendly.

[0010] To achieve the above object, the present invention adopts the following technical scheme: A bactericidal composition containing ethirimol sulfate, the bactericidal composition comprises active ingredient A and active ingredient B, the active ingredient A is ethirimol sulfate, and the active ingredient B is either Feneptamidoquin or Bifemetstrobin;

[0011] Further, the mass ratio of the active ingredient A to the active ingredient B is 1:45 to 60:1;

[0012] Further, the mass ratio of the ethirimol sulfate to Feneptamidoquin is 1:30 to 60:1;

[0013] Still further, the mass ratio of the ethirimol sulfate to Feneptamidoquin is 1:30 to 36:1;

[0014] Still further, the mass ratio of the ethirimol sulfate to Feneptamidoquin is 1:30, 1:16, 1:8, 7:2, 14:1, 28:1, 36:1, 60:1;

[0015] Further, the mass ratio of the ethirimol sulfate to Bifemetstrobin is 1:24 to 48:1;

[0016] Still further, the mass ratio of the ethirimol sulfate to Bifemetstrobin is 1:24 to 32:1

[0017] Furthermore, the mass ratio of ethirimol besylate to Bifemetstrobin is 1:24, 1:12, 1:6, 2:1, 6:1, 15:1, 32:1, 48:1;

[0018] Furthermore, based on 100 wt% of the total weight of the bactericidal composition, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 1 - 90 wt%;

[0019] Even further, based on 100 wt% of the total weight of the bactericidal composition, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 2 - 80 wt%;

[0020] Furthermore, in addition to the active ingredients, the bactericidal composition further comprises agriculturally acceptable auxiliary ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, thickeners, disintegrants, emulsifiers, defoamers, preservatives, stabilizers, synergists, carriers, solvents;

[0021] Furthermore, the wetting agent is selected from one or more of alkyl benzene sulfonates, alkyl naphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonates, alkyl phenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkyl phenol ethoxylates, fatty alcohol ethoxylates, fatty alcohol polyoxyethylene ether sulfates, silkworm excrement, Chinese honey locust powder, sapindus powder, SOPA, detergent, emulsifier 2000 series, and wetting penetrant F; and / or

[0022] Furthermore, the dispersant is selected from one or more of lignin sulfonates, alkyl naphthalene sulfonate formaldehyde condensates, naphthalene sulfonates, triphenylvinylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkyl phenol polyoxyethylene ethers, alkyl phenol polyoxyethylene ether methyl ether condensate sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylates, phosphates, EO-PO block copolymers, and EO-PO graft copolymers; and / or

[0023] Furthermore, the thickener is selected from one or more of xanthan gum, polyvinyl alcohol, organic bentonite, magnesium aluminum silicate, carboxymethyl cellulose; and / or

[0024] Furthermore, the disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0025] Further, the emulsifier is selected from one or more of fatty alcohol polyoxyethylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, phenethylphenol polyoxyethylene polypropylene ether, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, alkylbenzene sulfonate, styrylphenol polyoxyethylene ether, fatty acid polyoxyethylene ester; and / or

[0026] Further, the defoamer is selected from one or more of silicone oil, C 10 ~C 20 saturated fatty acid compounds, C 8 ~C 10 fatty alcohol compounds or silicone compounds; and / or

[0027] Further, the preservative is selected from one or more of sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate; and / or

[0028] Further, the stabilizer is selected from one or more of acid, succinic acid, adipic acid, borax, epoxidized vegetable oil; and / or

[0029] Further, the synergist is selected from synergistic phosphorus, synergistic ether; and / or

[0030] Further, the carrier is selected from one or more of kaolin, bentonite, attapulgite, light calcium carbonate, diatomite, silica; and / or

[0031] Further, the solvent is selected from one or more of benzene, toluene, xylene, methanol, ethanol, isopropanol, n-butanol, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, diesel oil, N,N-dimethylformamide, cyclohexanone, ethyl acetate, N-methylpyrrolidone, butyl ether, solvent oil, vegetable oil, vegetable oil derivatives and deionized water; and / or

[0032] Further, the bactericidal composition can be prepared into any preparation dosage form permitted in agriculture;

[0033] Further, the preparation dosage form is a solid preparation, a liquid preparation or a seed treatment preparation;

[0034] Further, the solid preparation is a directly used solid preparation, a dispersible solid preparation or a soluble solid preparation;

[0035] Furthermore, the directly used solid preparation is a powder, a granule, a ball, a tablet or a strip; the dispersible solid preparation is a wettable powder, an oil-dispersible powder, an emulsion powder, a water-dispersible granule, an emulsion granule or a water-dispersible tablet; the soluble solid preparation is a soluble powder, a soluble tablet or a soluble granule;

[0036] Furthermore, the liquid preparation is a solution preparation, a dispersion liquid preparation, an emulsion preparation, a suspension preparation or a multiphase preparation;

[0037] Still further, the solution preparation is a soluble agent, a solubilizable agent, an oil agent or a film-forming oil agent;

[0038] The dispersion liquid preparation is an emulsifiable concentrate, a latex, a dispersible liquid agent or an ointment;

[0039] The emulsion preparation is an aqueous emulsion, an oil emulsion, a microemulsion or a lipid preparation;

[0040] The suspension preparation is a suspending agent, a microcapsule suspension agent, an oil suspending agent or a dispersible oil suspending agent;

[0041] The multiphase preparation is a suspension-emulsion, a microcapsule suspension-suspending agent, a microcapsule suspension-aqueous emulsion or a microcapsule suspension-suspension-emulsion;

[0042] Furthermore, the seed treatment preparation includes a seed treatment solid preparation or a seed treatment liquid preparation;

[0043] Still further, the seed treatment solid preparation is a seed treatment dry powder agent or a seed treatment dispersible powder agent;

[0044] The seed treatment liquid preparation is a seed treatment liquid agent, a seed treatment emulsion or a seed treatment suspending agent;

[0045] Still further, the solid preparation is selected from water-dispersible granules or wettable powders, and the liquid preparation is selected from suspending agents, aqueous emulsions or emulsifiable concentrates.

[0046] Use of a bactericidal composition containing ethirimol blasticidin S and / or its preparation for controlling plant pathogenic bacteria.

[0047] Furthermore, the plant pathogenic bacteria are plant pathogenic fungi such as Basidiomycetes, Ascomycetes, Oomycetes and Deuteromycetes;

[0048] Furthermore, the oomycetes include Phytophthora, such as Phytophthora infestans, Phytophthora megasperma, Phytophthora parasitica, Phytophthora cinnamomi, and Phytophthora capsici; Pythium; Peronosporaceae such as Plasmopara viticola, Peronospora (including Peronospora tabacina and Peronospora parasitica); Pseudoperonospora and Bremia lactucae; Pythium such as Pythium aphanidermatum;

[0049] The Ascomycetes mentioned above include Alternaria, such as Alternaria solani and Alternaria brassicae; Guignardia, such as Guignardia bidwelli; Venturia, such as Venturia inaequalis; Septoria, such as Septoria nodorum and Septoria tritici; powdery mildew, such as Erysiphe (including Erysiphe graminis and Erysiphe polygoni), Uncinula necatur, Sphaerotheca fuligena (Erysiphe cichoracearum) and Podosphaera leucotricha; Botrytis, such as Botrytis cinerea and Monilinia fructicola; Sclerotinia, such as Sclerotinia sclerotiorum, Magnaporthe grisea, Phomopsis viticola; Helminthosporium, such as Helminthosporium triticirepentis; Pyrenophora teres; Colletotrichum, such as Colletotrichum graminicola and Colletotrichum orbiculare; Gaeumannomyces graminis;

[0050] The Basidiomycetes mentioned above include Puccinia spp., such as Puccinia recondita, Puccinia striiformis, Puccinia hordei, Puccinia graminis, and Puccinia arachidis; Hemileia vastatrix and Phakopsora pachyrhizi; other pathogens include Rhizoctonia spp., such as Rhizoctonia solani and Rhizoctonia oryzae; Fusarium spp., such as Fusarium roseum, Fusarium graminearum, and Fusarium oxysporum; Verticillium dahliae; Sclerotium rolfsii; Rynchosporium secalis; Cercospora arachidicola and Cercospora beticola;

[0051] Furthermore, the plant pathogenic bacteria and fungi are those on cereals, vegetables, fruits, and ornamental plants;

[0052] The bactericidal composition of the present invention is particularly suitable for controlling diseases caused by pathogenic bacteria on crop plants such as cereals (wheat, barley, rye, oats, rice, corn, sorghum, etc.), fruit trees (apple, pear, plum, peach, almond, cherry, banana, grape, strawberry, raspberry, blackberry, etc.), citrus trees (orange, lemon, tangerine, grapefruit, etc.), legumes (beans, peas, lentils, soybeans, etc.), vegetables (spinach, lettuce, asparagus, cabbage, carrot, onion, tomato, potato, eggplant, pepper, etc.), cucurbitaceae (pumpkin, zucchini, cucumber, melon, watermelon, etc.), oil plants (sunflower, rapeseed, peanut, castor, coconut, etc.), tobacco, coffee, tea, cocoa, sugar beet, sugar cane, cotton, etc.

[0053] Furthermore, the bactericidal composition and / or preparation are applied to the disease to be controlled or its growth medium in an effective dose.

[0054] Compared with the prior art, the beneficial effects of the technical solution of the present invention are as follows:

[0055] 1) The bactericidal composition of the present invention significantly improves the control effect on plant pathogenic bacteria and has an obvious synergistic effect.

[0056] 2) The bactericidal composition of the present invention can effectively reduce the dosage and application frequency of the medicament, and reduce the control cost.

[0057] 3) The bactericidal composition of the present invention can effectively control the generation of drug resistance by pathogenic microorganisms and can extend the service life of the single agent of the medicament. Detailed implementation manners

[0058] To make the technical solutions, objectives and advantages of the present invention clearer and more understandable, the present invention is described with the following specific embodiments, but the present invention can be implemented in various forms and should not be limited by the embodiments described herein.

[0059] In the following, the present invention has been described in detail with general descriptions and specific implementation manners. However, based on the present invention, some modifications or improvements can be made, which are obvious to those skilled in the art. All equivalent transformations made on the basis of the technical solutions of this application fall within the protection scope of the present invention.

[0060] Indoor bioactivity determination test

[0061] Example 1: Indoor activity determination test for cucumber powdery mildew

[0062] Test basis: The test refers to NY / T 1156.6-2006 "Pesticide indoor bioassay test guidelines" Part 6: Determination of combined effects of mixtures; fungicides.

[0063] NY / T 1156.11-2008 "Pesticide indoor bioassay test guidelines" Part 11: Test potted plant method for controlling cucurbit powdery mildew.

[0064] Test target: Erysiphe cichoracearum, the bacterial strain is taken from the group laboratory.

[0065] Test crops: Select the cucumber variety Xintaimici which is susceptible to powdery mildew, cultivate it in pots until the 4 true leaf stage, and number it for standby.

[0066] Test medicaments: 95% ethirimol besylate technical, 95% Bifemetstrobin technical, 95% Feneptamidoquin technical, and the above medicaments are all provided by the group R & D center.

[0067] Medicament preparation: First dissolve the above technicals with N,N-dimethylformamide, and then dilute them with 0.1% Tween-80 aqueous solution. Set 5 series of concentration gradients according to the activity of the medicaments.

[0068] Preparation of spore suspension: Spores on the powdery mildew-infected leaves were eluted with an aqueous solution containing 0.1% Tween-80, filtered through double-layer gauze, and formulated into a suspension with a spore concentration of 1×10 5 per mL for standby.

[0069] Inoculation and cultivation: The spore suspension was inoculated by spraying. The inoculated test materials were air-dried naturally and then transferred to a greenhouse for cultivation for 7 days under the condition of a temperature of 20°C - 24°C.

[0070] Pesticide treatment: The pesticide was evenly sprayed onto the standby cucumber seedlings by the spraying method and left to dry naturally. A treatment without pesticide was set as a blank control. There were 3 pots for each treatment and 4 replicates.

[0071] Data investigation: According to the disease incidence of the blank control, the disease incidence of each treatment was investigated by grading, and 30 leaves were investigated for each treatment.

[0072] The grading standard is as follows:

[0073] Grade 0: No disease spots;

[0074] Grade 1: The disease spot area accounts for less than 5% of the entire leaf area;

[0075] Grade 3: The disease spot area accounts for 6% - 15% of the entire leaf area;

[0076] Grade 5: The disease spot area accounts for 16% - 25% of the entire leaf area;

[0077] Grade 7: The disease spot area accounts for 26% - 50% of the entire leaf area;

[0078] Grade 9: The disease spot area accounts for more than 51% of the entire leaf area.

[0079] Data statistics and analysis:

[0080] The disease index was calculated according to formula (1)

[0081]

[0082] In the formula:

[0083] X - disease index;

[0084] N i - number of diseased leaves at each level;

[0085] i - relative grade value;

[0086] N - total number of leaves investigated.

[0087] The control effect was calculated according to formula (2)

[0088]

[0089] In the formula:

[0090] P—the control effect, in %;

[0091] CK—the disease index of the blank control;

[0092] PT—the disease index of the medicament treatment.

[0093] The synergistic effect of the mixed medicaments is evaluated according to Sun Yunpei's co-toxicity coefficient method (CTC), i.e., CTC ≤ 80 is antagonistic effect, 80 < CTC < 120 is additive effect, and CTC ≥ 120 is synergistic effect. The co-toxicity coefficient (CTC) is calculated according to formulas (3), (4), and (5).

[0094]

[0095] In the formulas:

[0096] ATI—the measured toxicity index of the mixture;

[0097] S—the EC 50 , in milligrams per liter (mg / L);

[0098] M—the EC 50 , in milligrams per liter (mg / L).

[0099] TTI = TI A ×P A +TI B ×P B ...........................................(4)

[0100] In the formulas:

[0101] TTI—the theoretical toxicity index of the mixture;

[0102] TI A —the toxicity index of medicament A;

[0103] P A —the percentage content of medicament A in the mixture, in milligrams per liter (mg / L);

[0104] TI B —the toxicity index of medicament B;

[0105] P B —the percentage content of medicament B in the mixture, in milligrams per liter (mg / L).

[0106]

[0107] In the formulas:

[0108] CTC - Co - toxicity coefficient;

[0109] ATI - Actual toxicity index of the mixture;

[0110] TTI - Theoretical toxicity index of the mixture.

[0111] The experiment used the DPS data processing system to process the data, established the toxicity regression equations respectively, and calculated the EC 50 value and the co - toxicity coefficient.

[0112] Test results:

[0113] The tested agents had high toxicity to the cucumber powdery mildew pathogen. The compound of ethirimol besylate and Feneptamidoquin showed good control effects against cucumber powdery mildew.

[0114] As shown in Table 1, when the mass ratio of ethirimol besylate to Feneptamidoquin was in the range of 1:30 - 60:1, the co - toxicity coefficients were all greater than 120, and the combined action showed synergism; when the mass ratio of ethirimol besylate to Feneptamidoquin was 1:30 - 36:1, the co - toxicity coefficient was greater than 150, and the synergistic effect was significant; when the mass ratio of ethirimol besylate to Feneptamidoquin was 7:2, the co - toxicity coefficient was the largest at 275.257, and the synergistic effect was the most significant.

[0115] Table 1 Indoor activity determination test of the mixture of ethirimol besylate and Feneptamidoquin against cucumber powdery mildew

[0116]

[0117] From the test results in Table 2, it can be seen that the compound of ethirimol besylate and Bifemetstrobin showed good control effects against cucumber powdery mildew. Among them, when the mass ratio of ethirimol besylate to Bifemetstrobin was in the range of 1:24 - 48:1, the co - toxicity coefficients were all greater than 150, and the combined action showed a synergistic effect; when the mass ratio of ethirimol besylate to Bifemetstrobin was 2:1, the co - toxicity coefficient was the largest at 238.807, and the synergistic effect was the most significant.

[0118] Table 2 Indoor activity determination test of the mixture of ethirimol besylate and Bifemetstrobin against cucumber powdery mildew

[0119]

[0120]

[0121] Preparation examples

[0122] Specific formulation composition and preparation method

[0123] Preparation Example 1: 18% ethirimol sulfonate·Feneptamidoquin emulsion in water (14:4)

[0124] Formulation: By weight percentage, 14% ethirimol sulfonate, 4% Feneptamidoquin, 8% EO-PO block copolymer, 20% cyclohexanone, 0.15% xanthan gum, 5% ethylene glycol, 0.5% sodium benzoate, 0.05% silicone defoamer, and deionized water to make up the balance.

[0125] Preparation method: According to the formulation design, mix the surfactant, antifreeze agent and water to form an aqueous phase, then dissolve ethirimol sulfonate and Feneptamidoquin with a solvent and add them to the aqueous phase under stirring. After stirring evenly, add the thickener and preservative and continue to shear for 10 min, then add the defoamer and stir evenly to form an O / W type emulsion in water.

[0126] Preparation Example 2: 12% ethirimol sulfonate·Feneptamidoquin emulsifiable concentrate (9:3)

[0127] Formulation: By weight percentage, 9% ethirimol sulfonate, 3% Feneptamidoquin, 15% N-methylpyrrolidone, 10% styrylphenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 10% cyclohexanone, 5% corn oil, and xylene to make up the balance.

[0128] Preparation method: Add the metered ethirimol sulfonate, Feneptamidoquin, solvent and cosolvent to a blending kettle and stir to dissolve them, then add the emulsifier and make up the balance with the remaining solvent. Stir evenly in a stirring kettle and filter to obtain the emulsifiable concentrate required by the present invention. Preparation Example 3: 30% ethirimol sulfonate·Feneptamidoquin suspending agent (21:9)

[0129] Formulation: By weight percentage, 21% ethirimol sulfonate, 9% Feneptamidoquin, 1% naphthalene sulfonate formaldehyde condensate, 1% alkylphenol polyoxyethylene ether, 3.5% sodium lignosulfonate, 1% polycarboxylate salt, 0.3% xanthan gum, 1.2% magnesium aluminum silicate, 0.5% fumed silica, 0.5% polyvinylpyrrolidone, 4% glycerol, 0.2% sodium benzoate, 0.5% silicone defoamer, and deionized water to make up the balance;

[0130] Preparation method: According to the formula ratio, the active ingredient ethirimol sulfate, Feneptamidoquin, surfactant and other functional auxiliaries are sequentially placed in a reaction kettle, mixed evenly with water, subjected to high-speed shearing and wet grinding, and finally homogenized and filtered to obtain the suspension product. Preparation Example 4: 20% Ethirimol Sulfate·Bifemetstrobin Emulsion in Water (15:5)

[0131] Formula: By weight percentage, 15% ethirimol sulfate, 5% Bifemetstrobin, 8% EO-PO block copolymer, 18% cyclohexanone, 0.15% xanthan gum, 5% glycerol, 0.5% sodium p-hydroxybenzoate, 0.1% silicone defoamer, and deionized water to make up the balance.

[0132] Preparation method: According to the formula design, the surfactant, antifreeze and water are mixed into the aqueous phase. Then, ethirimol sulfate and Bifemetstrobin are dissolved in a solvent and added to the aqueous phase under stirring. After stirring evenly, the thickener and preservative are added and sheared for 10 min, and then the defoamer is added and stirred evenly to form an O / W type emulsion in water.

[0133] Preparation Example 5: 24% Ethirimol Sulfate·Bifemetstrobin Suspension (20:4)

[0134] Formula: By weight percentage, 20% ethirimol sulfate, 4% Bifemetstrobin, 1.5% naphthalene sulfonate formaldehyde condensate, 1% alkylphenol polyoxyethylene ether, 3.5% sodium lignosulfonate, 1.5% calcium lignosulfonate, 0.2% xanthan gum, 1.2% magnesium aluminum silicate, 4% ethylene glycol, 0.5% fumed silica, 0.5% polyvinylpyrrolidone, 0.2% sodium p-hydroxybenzoate, 0.5% silicone defoamer, and deionized water to make up the balance;

[0135] Preparation method: According to the formula ratio, the active ingredient ethirimol sulfate, Bifemetstrobin, surfactant and other functional auxiliaries are sequentially placed in a reaction kettle, mixed evenly with water, subjected to high-speed shearing and wet grinding, and finally homogenized and filtered to obtain the suspension product.

[0136] Field efficacy test

[0137] Example 2: Field efficacy test of different agents against cucumber powdery mildew

[0138] Test site: Cucumber planting field in Qingzhou, Weifang City, Shandong Province, with moderate water and fertilizer management.

[0139] Test target: Cucumber powdery mildew pathogen.

[0140] Test crops, varieties and growth conditions: Cucumber (Jinyou No. 1), in the flowering and fruiting stage during the pesticide application period, with good growth.

[0141] Test pesticides: See the following table for test pesticides and application rates.

[0142] Table 3 Test pesticides and application rates

[0143]

[0144] Test settings: The plots of each pesticide treatment are distributed according to a randomized block design, with a protection row set around the plots. Each treatment has 4 replicates, and each plot is 20 m 2

[0145] Pesticide application method: Using the conventional spraying method, spray with a Lino brand HD400 type knapsack high-pressure sprayer, evenly spraying the liquid medicine on both the front and back of the leaves to make them wet but not dripping.

[0146] Investigation and statistical methods: The first pesticide application is carried out when the first disease spots are seen, and the pesticide is applied again after 7 days, with a total of two pesticide applications.

[0147] The disease incidence is investigated 7 days and 14 days after the last pesticide application. Randomly sample 5 points in each plot, select 10 diseased plants at each point, select 5 upper and middle leaves from each plant, label and fix the selected plants and leaves, grade each leaf according to the percentage of the disease spot area in the leaf area, and calculate the disease index and control effect.

[0148] The grading criteria are as follows:

[0149] Grade 0: No disease spots;

[0150] Grade 1: The disease spot area accounts for less than 5% of the whole leaf area;

[0151] Grade 3: The disease spot area accounts for 6%-10% of the whole leaf area;

[0152] Grade 5: The disease spot area accounts for 11%-20% of the whole leaf area;

[0153] Grade 7: The disease spot area accounts for 21%-40% of the whole leaf area;

[0154] Grade 9: The disease spot area accounts for more than 41% of the whole leaf area.

[0155] Pesticide efficacy calculation method:

[0156] The disease index and control effect are calculated using the following formula:

[0157]

[0158] In the formula: CK—Disease index after pesticide application in the blank control area;

[0159] PT—Disease index after spraying in the medicament treatment area.

[0160] Test results and analysis:

[0161] As can be seen from the following Table 4 of the field efficacy test, the compounding of ethirimol besylate with Feneptamidoquin or Bifemetstrobin shows good control effects against cucumber powdery mildew. For each mixing treatment, compared with the single agent, the control effects have been improved to varying degrees under the condition of reduced dosage. 7 days after spraying, the control effects of 18% ethirimol besylate·Feneptamidoquin aqueous emulsion (14:4), 30% ethirimol besylate·Feneptamidoquin suspension (21:9), 20% ethirimol besylate·Bifemetstrobin aqueous emulsion (15:5), and 24% ethirimol besylate·Bifemetstrobin suspension (20:4) against cucumber powdery mildew are 81.75%, 80.44%, 81.93%, and 83.18% respectively.

[0162] Table 4 Field efficacy test results of different medicaments against cucumber powdery mildew (7 days after spraying)

[0163]

[0164] As time increases, the control effect also becomes larger. 14 days after spraying, the control effects of 18% ethirimol besylate·Feneptamidoquin aqueous emulsion (14:4), 30% ethirimol besylate·Feneptamidoquin suspension (21:9), 20% ethirimol besylate·Bifemetstrobin aqueous emulsion (15:5), and 24% ethirimol besylate·Bifemetstrobin suspension (20:4) against cucumber powdery mildew are 82.37%, 82.70%, 85.09%, and 85.86% respectively.

[0165] Table 5 Field efficacy test results of different medicaments against cucumber powdery mildew (14 days after spraying)

[0166]

[0167] To sum up, through indoor toxicity determination and field efficacy tests, it can be seen that the bactericidal composition containing ethirimol besylate of the present invention has good control effects on plant pathogenic bacteria, is safe for target crops, has significant control effects, is superior to single agents in delaying the generation of drug resistance and prolonging the long-lasting effect, can effectively reduce costs, and reduce the residue of medicaments.

[0168] Although the present invention has been described in detail above with general descriptions and specific embodiments, based on the present invention, some modifications or improvements can be made, which are obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of the present invention claimed.

Claims

1. A bactericidal composition containing pyrimidine sulfonate, characterized in that: The bactericidal composition comprises active ingredient A and active ingredient B, wherein the active ingredient A is pyrimidine sulfonate, and the active ingredient B is any one of feneptamidoquin and bifemetstrobin.

2. The bactericidal composition according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:45 to 60:

1.

3. The bactericidal composition according to claim 1, characterized in that The mass ratio of the ethynol sulfonate to feneptamidoquin is 1:30 to 60:1; the mass ratio of the ethynol sulfonate to bifemetstrobin is 1:24 to 48:1; Preferably, the mass ratio of the bitumenol sulfonate to feneptamidoquin is 1:30 to 36:1; the mass ratio of the bitumenol sulfonate to bifemetstrobin is 1:24 to 32:

1.

4. The bactericidal composition according to claim 1, characterized in that The total weight of the bactericidal composition is 100 wt %, and the sum of the contents of the active ingredient A and the active ingredient B in the bactericidal composition is 1 to 90 wt %; Preferably, the total content of the active ingredient A and the active ingredient B in the bactericidal composition is 2 to 80 wt %.

5. The bactericidal composition according to claim 1, characterized in that In addition to the active ingredients, the fungicide composition also includes agriculturally permitted auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, thickeners, disintegrants, emulsifiers, defoamers, preservatives, stabilizers, synergists, carriers, and solvents.

6. The bactericidal composition according to claim 1, characterized in that The bactericidal composition can be prepared into any formulation form permitted in agriculture.

7. The bactericidal composition according to claim 6, characterized in that The dosage form of the preparation is a solid preparation or a liquid preparation; preferably, the solid preparation is a water-dispersible granule or a wettable powder, and the liquid preparation is a suspension, an emulsion in water or an emulsifiable concentrate.

8. Use of a fungicidal composition containing ethimol sulfonate and / or its preparation in controlling plant pathogenic bacteria.

9. The use according to claim 8, characterized in that The plant pathogenic bacteria are bacteria and fungi on cereals, vegetables, fruits and ornamental plants; preferably, the fungi are cucumber powdery mildew fungi.

10. The use according to claim 8, characterized in that The fungicidal composition and / or preparation is applied to the disease to be controlled or its growth medium at an effective dose.

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