Oral care composition for improving tooth remineralization effect and application thereof

Abstract

The invention relates to the technical field of oral care products, in particular to an oral care composition for improving the tooth remineralization effect and application of the oral care composition. The invention discloses an oral care composition for improving a tooth remineralization effect. The oral care composition comprises wheat active polypeptide and epsilon-polylysine in a weight ratio of (0.1-2): (0.05-0.5). The composition in the technical scheme can be applied to preparation of oral care products such as mouthwash, toothpaste, oral spray and tooth gel, and the effect of repairing enamel is achieved. According to the technical scheme, the technical problem that safe and efficient oral care products containing wheat active polypeptides are lacked in the prior art can be solved. Under the action of the wheat active polypeptide and epsilon-polylysine, tooth remineralization is promoted, and the corrosion resistance of the product is enhanced. The composition provided by the scheme is easy to be compatible with other common oral care components, the formula is convenient to adjust according to specific requirements, a product better meeting market requirements is developed, and the composition has ideal application prospects.

Description

Technical Field

[0001] The present invention relates to the technical field of oral care products, and in particular to an oral care composition for improving tooth remineralization effect and application thereof. Background Art

[0002] Diseases such as tooth erosion, caries and dentin sensitivity all originate from tooth demineralization, which seriously threatens people's oral health. Preventing tooth demineralization and improving its remineralization capacity is one of the key strategies to effectively deal with the above problems. Fluoride is widely used as the most recognized and effective remineralizing component. However, due to the dose-effect relationship of fluoride, although high concentrations of fluoride can provide excellent anti-caries effects, they may also cause chronic fluoride poisoning and even lead to the occurrence of fluorosis or skeletal fluorosis. When the oral pH value is lower than 5.5, hydroxyapatite will dissolve, causing tooth demineralization. In this process, saliva plays an important role. It can not only neutralize acidic substances in the oral cavity through components such as bicarbonate and phosphate to prevent and slow down demineralization, but also stably supply calcium ions, phosphate ions and fluoride elements to promote the natural remineralization of teeth.

[0003] Chinese invention patent CN108601956A introduces a mouthwash and its preparation method, revealing the potential of partially hydrolyzed plant protein (such as wheat hydrolyzed protein) in promoting tooth remineralization and enhancing the anti-caries effect of fluoride. Wheat active peptides, as a small molecule peptide mixture after wheat protein hydrolysis, can adhere to the surface of tooth enamel and adsorb mineral components in saliva, thereby enhancing the buffering capacity of saliva, replenishing minerals, and contributing to the natural remineralization of tooth enamel, showing a repair effect similar to fluoride. Therefore, adding wheat active peptides to oral care products can not only inhibit tooth demineralization and promote remineralization, but also replace traditional fluoride materials to avoid their potential toxic side effects.

[0004] However, wheat active peptide raw materials have a high risk of microbial contamination due to their natural origin and production process. The structure and properties of the peptide itself may also make it a good culture medium for microbial growth, further increasing the risk of product deterioration. In view of this, the development of oral care products with stable properties and containing wheat active peptides faces significant challenges. In order to ensure the effectiveness and safety of the product, effective preservative measures must be taken. This not only involves finding suitable preservatives to inhibit microbial growth, but also requires consideration of how to achieve this goal without affecting the biological activity of wheat active peptides. The ideal solution should be able to ensure the long-term stability of the product without affecting its effect of promoting tooth remineralization and inhibiting demineralization. Therefore, how to develop safe and efficient oral care products containing wheat active peptides to give full play to its inhibitory effect on tooth demineralization and promoting remineralization has become a key issue that needs to be urgently solved in this field. Summary of the invention

[0005] The purpose of the present invention is to provide an oral care composition for improving the tooth remineralization effect, so as to solve the technical problem of the lack of safe and efficient oral care products containing wheat active polypeptides in the prior art.

[0006] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0007] An oral care composition for improving tooth remineralization effect comprises wheat active polypeptide and ε-polylysine in a weight ratio of 0.1-2:0.05-0.5.

[0008] Furthermore, the composition of the wheat active polypeptides is as follows: >95% of the wheat active polypeptides have a molecular weight distribution of 0.5-10 kD, and >65% of the wheat active polypeptides have a molecular weight distribution of 0.5-3.5 kD.

[0009] Furthermore, the purity of the ε-polylysine is ≥94%, and the molecular weight distribution of the ε-polylysine is 3.6-4.3 kD.

[0010] The technical solution also provides an oral care product containing an oral care composition for improving the tooth remineralization effect.

[0011] Furthermore, the oral care product includes at least one of mouthwash, toothpaste, oral spray, and tooth gel.

[0012] Furthermore, in the oral care product, the added amounts of wheat active polypeptide and ε-polylysine are 0.1-2% and 0.05-0.5% respectively.

[0013] Furthermore, the oral care product also includes a matrix; the matrix includes at least one of a moisturizer, a thickener, a surfactant, a solubilizer, a sweetener, a preservative, a flavor and water.

[0014] Furthermore, the oral care product is a mouthwash; the raw materials of the mouthwash include 0.1-2 parts of wheat active polypeptide, 0.05-0.5 parts of ε-polylysine, 1-50 parts of moisturizer, 0.1-20 parts of solubilizer, and 0.1-5 parts of flavor, by weight.

[0015] The technical solution also provides a method for preparing an oral care product, comprising the following steps performed in sequence:

[0016] S1: Stir the moisturizer and water until homogenous, add the solubilizer and stir until homogenous;

[0017] S2: Add wheat active polypeptide and ε-polylysine in sequence and stir evenly;

[0018] S3: Add essence and mix well to make mouthwash.

[0019] The technical solution also provides an application of an oral care product in the preparation of a product for repairing tooth enamel.

[0020] In summary, the technical principle of this technical solution is:

[0021] The oral care composition for improving tooth remineralization proposed by the present invention is mainly achieved by the compound use of wheat active polypeptide and ε-polylysine. Wheat active polypeptide can adhere to the surface of tooth enamel, absorb calcium ions, phosphate ions and other mineral components in saliva, thereby enhancing the buffering capacity of saliva, replenishing minerals, and contributing to the natural remineralization of tooth enamel. ε-polylysine, as a broad-spectrum antibacterial agent of natural origin, can not only effectively inhibit the growth of microorganisms and reduce the risk of product deterioration during storage, but also, although ε-polylysine itself does not have the performance of promoting mineralization, it can further promote the biological activity of wheat active polypeptide with wheat active polypeptide.

[0022] In the composition, wheat active polypeptides (>95% of the wheat active polypeptides have a molecular weight distribution of 0.5-10kD, and >65% of the wheat active polypeptides have a molecular weight distribution of 0.5-3.5kD) and ε-polylysine (purity ≥94%, molecular weight distribution of 3.6-4.3kD) are mixed in a specific ratio to form a synergistic effect between the two, which can effectively promote tooth remineralization and provide necessary anticorrosion protection. The inventor further analyzed that the reason for the synergistic effect between the two may be that a certain proportion of wheat active polypeptides and polylysine are mixed, and the two are adsorbed on the surface of tooth enamel and work together to produce specific physical and chemical bonding, thereby facilitating the enrichment of calcium and phosphorus ions in saliva on the tooth surface, crystal nucleation, growth aggregation and other processes, and ultimately improving the mineralization effect of tooth enamel.

[0023] The beneficial effects of this technical solution are:

[0024] Highly effective in promoting tooth remineralization: Through the action of wheat active peptides, the composition can significantly improve the remineralization effect of teeth, help repair early caries damage, and prevent problems such as erosion and dentin sensitivity. Compared with traditional fluoride, this method avoids the toxic side effects that may be caused by high concentrations of fluoride.

[0025] Enhanced antiseptic properties: ε-polylysine, as a natural antibacterial agent, can not only effectively inhibit the growth of microorganisms and ensure the safety and stability of the product, but can also be decomposed into lysine in the human body. It is safer than other chemical preservatives and is suitable for long-term use.

[0026] Synergistic effect: Studies have found that the specific ratio of ε-polylysine and wheat active peptides can not only bring out their respective advantages, but also produce a synergistic effect, further enhance the effect of tooth remineralization, and improve the overall stability and effectiveness of the product.

[0027] Wide application: The composition is suitable for a variety of oral care products, such as mouthwash, toothpaste, oral spray, tooth gel, etc., to meet the needs of different users and expand the scope of application.

[0028] Formula flexibility: The composition is easily compatible with other commonly used oral care ingredients (such as moisturizers, thickeners, surfactants, etc.), making it easy to adjust the formula according to specific needs and develop products that better meet market needs. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 These are scanning electron micrographs of the bovine enamel surfaces in the self-control acid-etched areas and remineralized areas of the experimental group 2, the control group 2-1, the control group 2-2, and the control group 6 of Experimental Example 2. DETAILED DESCRIPTION

[0030] The present invention is further described in detail below in conjunction with examples, but the embodiments of the present invention are not limited thereto. Unless otherwise specified, the technical means used in the following examples and experimental examples are conventional means well known to those skilled in the art, and the materials, reagents, etc. used can be obtained from commercial sources.

[0031] Example 1: Preparation of oral care composition

[0032] (1) Oral Care Composition

[0033] The oral care composition is composed of the following raw materials: wheat active polypeptide and ε-polylysine in a mass ratio of 0.1-2:0.05-0.5.

[0034] If the oral care composition is used to prepare oral care products, every 100 parts of the oral care products contain 0.1-2 parts of wheat active polypeptide and 0.05-0.5 parts of ε-polylysine, that is, the mass percentages of wheat active polypeptide and ε-polylysine are 0.1-2% and 0.05-0.5%, respectively. Oral care products include mouthwash, toothpaste, oral spray, tooth gel, etc.

[0035] Among them, wheat active polypeptides (purchased from BASF, more specifically, BASF's Enameguard natural hydrolyzed wheat protein product) and ε-polylysine (more specifically, ε-polylysine purchased from Zhengzhou Bainafo Bioengineering Co., Ltd.) can be obtained through commercial means.

[0036] Wheat active peptides can effectively adhere to the surface of tooth enamel, absorb calcium ions, phosphate ions and other minerals in saliva, thereby enhancing the buffering capacity of saliva, and replenishing the mineral ions required by teeth, which is conducive to the natural remineralization of tooth enamel. In this scheme, more than 95% of the wheat active peptides have a molecular weight distribution in the range of 0.5-10kD, and more than 65% of the wheat active peptides have a molecular weight distribution in the range of 0.5-3.5kD. ε-Polylysine is a natural biological preservative produced by Streptomyces fermentation, and its main component is lysine monomers connected by peptide bonds. In this scheme, ε-Polylysine with a molecular weight of 3.6-4.3kD and a purity of not less than 94% is specifically used. Adding ε-Polylysine to oral care products, especially oral care products such as those containing hydrolyzed wheat protein, can provide additional antiseptic protection and reduce the risk of microbial contamination.

[0037] (2) Oral care products containing oral care compositions

[0038] The oral care product comprises an oral care composition and a matrix, wherein the matrix comprises at least one of a conventional humectant, a thickener, a surfactant, a solubilizer, a sweetener, a preservative, an aroma modifier (flavor) and water. Preferably, when preparing a mouthwash, the mouthwash comprises 0.1-2 parts of wheat active polypeptide, 0.05-0.5 parts of ε-polylysine, 1-50 parts of a humectant, 0.1-20 parts of a solubilizer, and 0.1-5 parts of a flavor.

[0039] When the oral care product is a mouthwash, the preparation method is as follows:

[0040] S1: Stir the moisturizer and water until homogenous, add the solubilizer and stir until homogenous;

[0041] S2: Add the oral care composition and stir evenly. The oral care composition is added by sequentially adding wheat active polypeptide and ε-polylysine, and mixing and stirring until the mixture is evenly dispersed and clear.

[0042] S3: Add essence and mix well to make mouthwash.

[0043] Mouthwash was prepared according to the formula in Table 1 and the above-mentioned preparation method. In the specific experiment, wheat active polypeptide was Enameguard from BASF, and ε-polylysine was purchased from Zhengzhou Bainafo Bioengineering Co., Ltd. (ε-polylysine purity>94%, molecular weight distribution range of 3.6-4.3kD). Glycerin (humectant), sorbitol (humectant), poloxamer (solubilizer) and mint flavor were added to the formula of the mouthwash. It should be noted that, in addition to adding the oral care composition of the present scheme (wheat active polypeptide and ε-polylysine in a mass ratio of 0.1-2:0.05-0.5) to conventional mouthwash in the prior art, the above-mentioned oral care composition can also be added to conventional toothpaste, oral spray and tooth gel in the prior art. The oral care composition can achieve good antiseptic and antibacterial effects and repair enamel (enamel remineralization) in carriers such as mouthwash, toothpaste, oral spray and tooth gel, and the wheat active polypeptide and ε-polylysine can show a synergistic effect in promoting enamel mineralization in the above-mentioned conventional oral care products.

[0044] Table 1: Mouthwash components (unit: g; the total amount of raw materials in each example is 100 g)

[0045]

[0046]

[0047] Experimental Example 1: Mouthwash Antiseptic Challenge Test

[0048] The test method refers to the USP method (United States Pharmacopeia). The test samples were placed in a sterile container, and mixed bacterial suspensions (Escherichia coli in logarithmic growth period, Staphylococcus aureus, and Pseudomonas aeruginosa in a volume ratio of 1:1:1) and fungal suspensions (Candida albicans and Aspergillus niger spores) were added. The colony count results showed that the bacterial concentration of each test sample was 6.8×10 6 The concentration of fungal culture liquid is 6.05×10 5 , the concentration of the bacterial solution met the test requirements. The samples were gently mixed to ensure uniform microbial distribution and cultured in the dark at 20-25°C. Colony counts were performed after contact times of 14 and 28 days, respectively. The evaluation criteria for passing the antiseptic challenge are: the logarithmic value of bacteria decreases by ≥2 in 14 days and does not increase in 28 days; and the logarithmic value of fungi does not increase in 14 and 28 days. For samples whose logarithmic values ​​cannot pass the evaluation criteria after the 14-day colony count, the subsequent 28-day colony count will not be performed. See Table 2 for the experimental strain culture conditions and Table 3 for the antiseptic challenge test results.

[0049] Table 2: Experimental strain culture conditions

[0050] strain Culture medium Cultivation temperature Cultivation time Staphylococcus aureus ATCC 6538 TSA 36℃±1℃ 18h-24h Escherichia coli ATCC 8739 TSA 36℃±1℃ 18h-24h Pseudomonas aeruginosa ATCC 9027 TSA 36℃±1℃ 18h-24h Aspergillus niger ATCC 16404 SDA 28℃±2℃ 3d-5d Candida albicans ATCC 10231 SDA 28℃±2℃ 48h-72h

[0051] Table 3: Corrosion Challenge Test Results

[0052]

[0053]

[0054] The test results show that except for experimental group 4, control group 1-2, control group 2-2, control group 3, control group 4, control group 5, and control group 6, all other mouthwash samples can pass the antiseptic challenge test, indicating that the ε-polylysine concentration in the mouthwash can play a good antiseptic effect within the range of 0.05-0.5%. The addition of ε-polylysine solves the stability problem of wheat active polypeptides, making the antiseptic performance of oral care products containing wheat active polypeptides more ideal.

[0055] Experimental Example 2: Remineralization Experiment

[0056] (1) Experimental principle: Use 1% citric acid (pH 3.5) to etch bovine tooth enamel for demineralization. Then soak the demineralized samples in mouthwash. Detect the hardness of bovine tooth enamel before and after remineralization. Whether there is a significant difference compared with the control is used to evaluate the remineralization effect of mouthwash.

[0057] (2) Reagent preparation: ① 1% citric acid: weigh 10 g of anhydrous citric acid powder, dissolve it in 800 mL of deionized water, add sodium hydroxide solution to adjust its pH to 3.5, and finally use deionized water to adjust the volume to 1 L. ② Artificial saliva: 2.2 g / L gastric mucin, 0.381 g / L NaCl, 0.426 g / L CaCl 2 ·2H 2 O, 1.476g / LK 2 HPO 4 ·3H 2 O, 1.114 g / L KCl. First, stir MUCIN until it is completely dissolved and sterilized, then add other ingredients in sequence, stir to dissolve and make up to volume, and finally adjust the pH to 7.0 with 85% lactic acid solution.

[0058] (3) Operation steps: Screen hardness value between (230-360) kg / mm 2 For each piece of tooth enamel sample, 10 mL of 1% citric acid was added and soaked for 10 minutes to complete the acid etching of the tooth enamel sample. The acid-etched sample was placed in deionized water at 4°C for later use. The hardness value of the acid-etched tooth enamel sample was marked as HV 脱矿后 Then, the extracorporeal circulation treatment was performed according to the steps in Table 4. The hardness value of the acid-etched enamel sample after extracorporeal circulation treatment was marked as HV. 再矿化Five points were selected for each enamel sample to measure the hardness value, and the average value of the five measurement points was taken as the hardness of the enamel sample. Each embodiment or comparative example included six enamel samples.

[0059] Table 4: Extracorporeal circulation steps

[0060]

[0061] (4) Determination of the microhardness of bovine tooth enamel surface: Calculate the change in microhardness value of bovine tooth enamel before and after remineralization △HV = HV 再矿化 -HV 脱矿后 , using independent sample t test, if the △HV value of the mouthwash in the other experimental groups is greater than the △HV value of the control group 6, and there is a statistically significant difference between the △HV values ​​of the two groups (P < 0.05), then the mouthwash is considered to have the effect of promoting the remineralization of tooth enamel. The experimental results are shown in Table 5.

[0062] Table 5: Microhardness test results of bovine enamel surface (* indicates: independent sample t test, compared with control group 6, p < 0.05; average HV 脱矿后 The average hardness of bovine enamel before mineralization in repeated experiments. 再矿化 The average hardness of bovine tooth enamel after repeated mineralization experiments; in each group of experiments, the △HV of each bovine tooth enamel sample before and after mineralization was calculated, and then the average value was taken, which is the average △HV)

[0063] Grouping <![CDATA[Average HV 脱矿后 > <![CDATA[Average HV 再矿化 > Average △HV Experimental Group 1 185.6±18.7 217.5±12.5 31.9±5.3* Experimental Group 2 189.3±11.1 243.7±16.2 54.4±8.4* Experimental Group 3 184.2±13.9 253.4±17.6 69.2±13.2* Experimental Group 4 185.9±18.2 201.3±15.6 15.4±5.7* Experimental Group 5 188.7±14.3 241.2±21.8 52.5±11.5* Control group 1-1 186.8±15.1 195.3±9.7 8.5±2.2 Control group 1-2 188.4±17.3 205.3±16.9 16.9±4.6* Control group 2-1 184±12.8 196.6±13.4 11.6±2.8 Control group 2-2 189.1±12.5 215.8±16.1 26.8±8.7* Control group 2-3 187.5±9.4 212.6±19.7 25.1±6.1* Control group 3 186.2±15.2 226.9±16.3 40.7±9.7* Control group 4 184.4±18.2 193.1±11.5 8.7±2.9 Control group 5 187.5±19.6 233.6±13.7 46.1±9.4* Control group 6 189.8±19.9 201.1±17.0 9.8±2.5

[0064] From the results in Table 5, we can see that:

[0065] ① Compared with the control group 6, the microhardness of the experimental groups 1, 2 and 3 increased significantly, and was positively correlated with the concentration of the composition, indicating that as the concentration of the composition increased, the remineralization effect of the composition increased accordingly.

[0066] ② Compared with control group 6, the microhardness values ​​of control group 1-1, control group 2-1, and control group 4 had no significant changes, indicating that the mouthwash containing ε-polylysine alone had no significant remineralization effect.

[0067] The mechanism of bioactive peptides regulating biomineralization has not yet been revealed. Studies have shown that factors such as peptide sequence, length, side group structure, and secondary and tertiary spatial structure of the peptide will affect its biomineralization performance. Chinese patent CN112480400B discloses a poly-lysine grafted modified mineralization crystal growth inhibitor and its preparation method and application. 3The anhydride of the polylysine generates a mineralized crystal nucleation and growth inhibitor, which inhibits the nucleation and growth of mineral crystals during the mineralization process. Chinese patent CN114573806B discloses a polylysine grafted modified mineralized crystal nucleation and growth promoter, and its preparation method and application. A mineralized crystal nucleation and growth promoter is generated by grafting an acid anhydride containing a -COOH group on the side chain of polylysine to promote the nucleation and growth of mineral crystals during the mineralization process. At present, the biomineralization properties of different active polypeptides are still difficult to predict. Whether each active polypeptide has a mineralization effect and its mechanism of action still need to be further explored. Polylysine will show the effect of inhibiting or promoting mineralization according to different grafting methods. The experimental results of this scheme show that ε-polylysine does not undergo any grafting treatment and has no significant effect on mineralization itself.

[0068] ③ Compared with control group 6, the microhardness of control group 1-2, control group 2-2, control group 3, and control group 5 increased significantly, indicating that the mouthwash containing wheat active polypeptides has a remineralization effect. There was no significant difference between control group 2-2 and control group 2-3 (P < 0.05), indicating that the preservative sodium benzoate did not have a significant effect on the mineralization of wheat active polypeptides, but simply acted as a preservative.

[0069] ④ The △HV value of experimental group 1 is greater than the sum of the △HV values ​​of control group 1-1 and control group 1-2 [31.9>(8.5+16.9=25.4)]. 0.05 parts of ε-polylysine itself does not have the effect of promoting mineralization (there is no significant difference between the △HV of control group 1-1 and the △HV of group 6 that does not use wheat active polypeptide and ε-polylysine). However, after using 0.05 parts of ε-polylysine and 0.1 parts of wheat active polypeptide together (experimental group 1), it was found that the △HV of experimental group 1 was increased by nearly 90% relative to the △HV of control group 1-2 (only 0.1 parts of wheat active polypeptide were used without adding ε-polylysine). This shows that ε-polylysine has the effect of promoting the mineralization effect of wheat active polypeptide when it does not have the effect of promoting mineralization itself.

[0070] The △HV value of experimental group 2 was greater than the sum of the △HV values ​​of control group 2-1 and control group 2-2 [54.4>(11.6+26.8=38.4)], and the △HV value of experimental group 3 was greater than the sum of the △HV values ​​of control group 2-1 and control group 3 [69.2>(11.6+40.7=52.3)]. The above experimental data also show that ε-polylysine has the effect of promoting the mineralization effect of wheat active polypeptide, that is, ε-polylysine and wheat active polypeptide have a synergistic effect in promoting tooth remineralization.

[0071] ⑤ The △HV (15.4) of experimental group 4 (0.1 part of wheat active polypeptide and 0.02 part of ε-polylysine) is less than the sum of the △HV values ​​of control groups 1-2 (0.1 part of wheat active polypeptide) and control group 4 (0.02 part of ε-polylysine) (16.9+8.7=25.6). This shows that under this ratio (too little ε-polylysine), the synergistic effect of wheat active polypeptide and ε-polylysine does not exist.

[0072] The △HV (52.5) ​​of experimental group 5 (2.5 parts of wheat active polypeptide and 0.5 parts of ε-polylysine) is less than the sum of the △HV values ​​of control group 2-1 (0.5 parts of ε-polylysine) and control group 5 (2.5 parts of wheat active polypeptide) (11.6+46.1=57.7). This shows that under this ratio (excessive dosage of wheat active polypeptide), the synergistic effect of wheat active polypeptide and ε-polylysine does not exist.

[0073] Therefore, experimental groups 4 and 5 showed that ε-polylysine and wheat active polypeptide had a synergistic mineralization effect only within a specific ratio range.

[0074] In summary, the weight ratio of wheat active polypeptide to ε-polylysine is in the range of 0.1-2:0.05-0.5. The combination of the two has a synergistic mineralization effect while solving the problem of wheat active polypeptide being susceptible to microbial contamination. The combination is better than the effect of using a single component. In the prior art, ε-polylysine is only used as a preservative. However, in the application scenario of this solution, ε-polylysine and wheat active polypeptide have a certain mutual influence, which greatly promotes the mineralization effect of wheat active polypeptide and produces unexpected technical effects.

[0075] (5) Scanning electron microscopy observation of bovine tooth enamel surface: The scanning electron microscopy images of bovine tooth enamel surface after remineralization treatment with different formulas of mouthwash are shown in detail. Figure 1 (Showing microscopic images of the enamel surfaces of experimental group 2, control group 2-1, control group 2-2 and control group 6 after acid etching and with or without restoration treatment), the mouthwash formulas of the experimental group and the control group are shown in Table 1. As shown in the figure, a large number of parallel enamel column fiber bundles in the acid-etched self-control area of ​​each sample are exposed, making the enamel surface scaly. Since the enamel column interstitial is more susceptible to acid etching, it can be clearly seen from the figure that the enamel column interstitial has basically disappeared, and the gaps between the enamel columns have increased significantly.

[0076] The comparative observation results show that:

[0077] ① Compared with the sample acid-etched control area and control group 6, the amount of mineral deposition on the enamel surface after remineralization (repair) in experimental group 2 increased significantly, and a thin mineralized layer was formed covering the enamel surface, indicating that the mouthwash containing wheat active peptides and ε-polylysine has a significant remineralization effect. Similarly, the mouthwashes in experimental groups 1 and 3 also showed similar effects to the mouthwash in experimental group 2.

[0078] ② There was no obvious mineral deposition in the acid-etched control area and mineralized area of ​​the samples of control group 6 and control group 2-1, indicating that the mouthwash without the composition and the mouthwash containing ε-polylysine alone had no significant mineralizing effect.

[0079] ③Compared with the sample's own control acid-etched area, a certain amount of mineral deposition can be observed in the mineralized area of ​​the control group 2-2, indicating that the mouthwash containing wheat active peptides alone has a certain mineralization effect.

[0080] ④ Compared with the control group 2-2, the mineral deposition amount of the experimental group 2 increased significantly and a continuous thin mineralized layer appeared covering the surface of the enamel fiber bundles. The above comparison results indicate that the remineralization effect of the combination is better than that of a single component in the combination. ε-polylysine is added to the mouthwash as a preservative. In addition to its own preservative effect, it can significantly enhance the mineralization efficacy of the mouthwash containing wheat active polypeptides.

[0081] Experimental Example 3: Stability Experiment

[0082] For the mouthwash samples of experimental groups 1-3 in Table 1, stability tests were carried out in accordance with GB / T 43543-2023, including heat stability (40°C), cold stability (4°C), and pH stability. The results of the 90-day stability test are shown in Table 6. The results show that the three mouthwashes have good stability, and the composition (wheat active polypeptide + ε-polylysine) provided in this scheme is easily compatible with other commonly used oral care ingredients. In addition to being prepared as a mouthwash, the composition of this scheme is added to conventional toothpaste, oral spray, tooth gel, etc., and the overall product also has corresponding stability.

[0083] Table 6: Stability test results

[0084]

[0085] The above is only an embodiment of the present invention, and the common knowledge such as the known specific structure and characteristics in the scheme is not described in detail here. It should be pointed out that for those skilled in the art, several deformations and improvements can be made without departing from the structure of the present invention, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. An oral care composition for improving tooth remineralization effect, characterized in that: The invention comprises wheat active polypeptide and ε-polylysine in a weight ratio of 0.1-2:0.05-0.

5.

2. An oral care composition for improving tooth remineralization effect according to claim 1, characterized in that: The composition of the wheat active polypeptides is as follows: >95% of the wheat active polypeptides have a molecular weight distribution of 0.5-10 kD, and >65% of the wheat active polypeptides have a molecular weight distribution of 0.5-3.5 kD.

3. An oral care composition for improving tooth remineralization effect according to claim 2, characterized in that: The purity of the epsilon-polylysine is ≥94%, and the molecular weight distribution of the epsilon-polylysine is 3.6-4.3 kD.

4. An oral care product comprising the oral care composition for improving tooth remineralization effect according to any one of claims 1 to 3.

5. The oral care product according to claim 4, characterized in that: The oral care product includes at least one of mouthwash, toothpaste, oral spray and tooth gel.

6. The oral care product according to claim 5, characterized in that: In the oral care product, the added amounts of wheat active polypeptide and ε-polylysine are 0.1-2% and 0.05-0.5% respectively.

7. The oral care product according to claim 6, characterized in that: The oral care product further comprises a matrix; the matrix comprises at least one of a moisturizer, a thickener, a surfactant, a solubilizer, a sweetener, a preservative, a flavor and water.

8. The oral care product according to claim 7, characterized in that: The oral care product is a mouthwash; the raw materials of the mouthwash include 0.1-2 parts of wheat active polypeptide, 0.05-0.5 parts of ε-polylysine, 1-50 parts of moisturizer, 0.1-20 parts of solubilizer, and 0.1-5 parts of flavor, in parts by mass.

9. The method for preparing the oral care product according to claim 8, characterized in that: The method includes the following steps: S1: Stir the moisturizer and water until homogenous, add the solubilizer and stir until homogenous; S2: Add wheat active polypeptide and ε-polylysine in sequence and stir evenly; S3: Add essence and mix well to make mouthwash.

10. Use of the oral care product according to claim 4 in preparing a product for repairing tooth enamel.

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