Pseudomonas monteilii strain psm006 and applications thereof

Abstract

The application belongs to the technical field of microorganisms, and particularly relates to a Pseudomonas monteilii psm006 and application thereof. Pseudomonas monteilii The Pseudomonas monteilii psm006 has been preserved in the China General Microbiological Culture Collection Center (CGMCC) on January 3, 2025, and the address of the CGMCC is No. 1, Yihuangyuan, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO. 33294. The application provides the Pseudomonas monteilii psm006, which can survive in a certain saline-alkali environment, has good silicate dissolution capacity, certain phosphorus dissolution and nitrogen fixation capacity, and can secrete auxin and the like to improve the germination rate of Chinese cabbage seeds in a salt environment.

Description

Technical Field

[0001] This application belongs to the field of microbial technology, specifically relating to a strain of Pseudomonas montmorillonii psm006 and its applications. Background Technology

[0002] Currently, due to human land reclamation, over-irrigation, and other factors related to climate change, over 6% of the world's total land area is affected by salinization. Salinization has a very serious impact on both soil and plants. Saline-alkali soils are characterized by high salt content and strong alkalization, leading to reduced soil moisture content and poor soil aeration, resulting in a condition that is "muddy when wet and compacted when dry." The harm caused by soil salinization is mainly due to two reasons: high concentrations of salt ions in the soil cause an imbalance in osmotic pressure in plants, thereby reducing root water absorption and leading to internal dehydration, similar to dehydration caused by drought. Simultaneously, high concentrations of salt ions also inhibit the absorption of nutrients such as phosphorus, potassium, silicon, and nitrogen by plants. Furthermore, the accumulation of salt ions in the soil leads to ion toxicity, directly interfering with the growth and metabolic processes of plant cells, thus affecting plant growth.

[0003] Existing methods for improving saline-alkali soil mainly include physical, chemical, and microbial methods. Among physical methods, underground pipe drainage is a relatively feasible and effective approach. This involves laying underground pipes at a certain depth in the soil, utilizing rainwater or irrigation water to dissolve and drain salts from the soil, thereby reducing salt concentration. However, this method is costly in terms of manpower and resources, making it less economical and environmentally friendly. Chemical methods, on the other hand, can cause serious environmental pollution if chemical amendments are overused. To ensure the continued healthy growth of crops in saline-alkali soil, additional fertilizers are frequently needed to meet their nutritional requirements. These chemical fertilizers remain in the soil for extended periods, having long-term environmental impacts, affecting soil fertility, crop health, and carbon footprint, leading to eutrophication and heavy metal accumulation, ultimately harming human health. Therefore, there is an urgent need to develop a green improvement method. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide a strain of *Pseudomonas montelukastii* psm006 and its applications, specifically through the following technical solution:

[0005] In a first aspect, the present invention provides a strain of *Pseudomonas montmorillonii* psm006, which is a strain of *Pseudomonas montmorillonii* (… Pseudomonas monteilii psm006 was deposited on January 3, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC NO. 33294.

[0006] This invention screened a highly efficient silica-solubilizing strain, *Pseudomonas montmorilloni* psm006, from soil. This strain also exhibits certain salt and alkali tolerance and growth-promoting abilities. This invention investigated the effects of this strain on Chinese cabbage seeds by simulating a saline environment. The results showed that PSM006 could promote the germination rate of Chinese cabbage seeds under saline conditions.

[0007] The 16S rDNA gene sequence of the above-mentioned Pseudomonas montelukastii psm006 is shown in SEQ ID No. 1.

[0008] SEQ ID No. 1:

[0009]

[0010] Secondly, the present invention provides the application of the above-mentioned Pseudomonas montmorillonii psm006 or its bacterial solution in promoting the germination of Chinese cabbage seeds and / or in the preparation of Chinese cabbage seed germination promoters.

[0011] Thirdly, the present invention provides a germination promoter for Chinese cabbage seeds, which includes the aforementioned Pseudomonas montmorillonii psm006.

[0012] Fourthly, the present invention provides the application of the above-mentioned Pseudomonas montmorillonii psm006 or its bacterial culture in the degradation of silica and / or the preparation of silica degrading agents.

[0013] Fifthly, the present invention provides a silicate degrading agent comprising the aforementioned Pseudomonas montmorillonii psm006.

[0014] In a sixth aspect, the present invention provides the use of the above-mentioned Pseudomonas montmorillonii psm006 or its bacterial suspension in inhibiting plant pathogens and / or preparing plant pathogen inhibitors.

[0015] As a further preferred embodiment, the plant pathogens mentioned above include the pathogens causing corn stalk rot or the pathogens causing wheat take-all disease.

[0016] In a seventh aspect, the present invention provides a plant pathogen inhibitor, which includes the aforementioned Pseudomonas montmorillonii psm006 or its bacterial suspension.

[0017] Eighthly, embodiments of this application provide a method for inhibiting plant pathogens, wherein the above-mentioned Pseudomonas montmorillonii psm006 or its bacterial solution is used to treat the plant, the plant pathogens including the corn stalk rot pathogen or the wheat take-all pathogen.

[0018] The beneficial effects of this invention are as follows:

[0019] This invention provides a strain of *Pseudomonas montmorillonii* psm006, which can survive in certain saline-alkali environments and has good silicate-dissolving ability as well as certain phosphorus-solubilizing and nitrogen-fixing abilities. Furthermore, the strain can secrete auxins to improve the germination rate of cabbage seeds under saline conditions. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0021] Figure 1The colony morphology (a) and Gram staining (b) of Pseudomonas montmorillonii psm006 are shown. Scale bar: 1 μm.

[0022] Figure 2 The image shows the phylogenetic tree of Pseudomonas montmorillonii psm006;

[0023] Figure 3 The image shows the growth of Pseudomonas montmorillonii psm006 on a silica-dissolving plate (a) and the silica-dissolving hydrolysis zone (the dashed line in b).

[0024] Figure 4 The image shows the determination of the silica-dissolving ability of Pseudomonas montmorillonii psm006 in liquid.

[0025] Figure 5 The figure shows the growth of Pseudomonas montmorillonii psm006 in LB liquid medium containing different concentrations of NaCl.

[0026] Figure 6 The figure shows the growth of Pseudomonas montmorillonii psm006 in LB liquid medium at different pH values.

[0027] Figure 7 The image shows the phosphate hydrolysis zone of Pseudomonas montmorillonii psm006;

[0028] Figure 8 The image shows the nitrogen-fixing hydrolysis zone of Pseudomonas montmorillonii psm006;

[0029] Figure 9 The figure shows the size of the hydrolytic hydrolysis zone of Pseudomonas montmorillonii psm006 strain under different saline-alkali conditions: (a) under mild (3% NaCl) saline-alkali conditions; (b) under moderate (6% NaCl) saline-alkali conditions. The solid red lines in the figure represent colonies, and the dashed red lines represent the hydrolytic hydrolysis zone.

[0030] Figure 10 The results of the experiment on the promotion of cabbage seed germination by Pseudomonas montmorilloni psm006 are shown. Scale bar: 1cm.

[0031] Figure 11 The results of Pseudomonas montmorillonii psm006 inhibiting pathogens are shown: (a) Pathogen of maize stalk rot: Fusarium moniliforme ( Fusarium verticillioides (b) Pathogen of wheat take-all disease: *Oat vulgaris* (*Oat vulgaris*) Gaeumonnomyces graminis var avenae ). Detailed Implementation

[0032] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0033] Example 1

[0034] Screening and identification of Pseudomonas montelukastii psm006

[0035] The experimental samples of this invention were collected from plant rhizosphere soil in the saline-alkali land of Dalad Banner, Inner Mongolia. The specific implementation method is as follows:

[0036] 1. Preparation of soil suspension: Weigh 10.0g of soil sample and add it to a 250mL shake flask containing 90mL of sterile water (this process must be performed under aseptic conditions). Place the flask in a shaker and shake (180 rpm, 2-3 hours) to obtain 10... -1 Soil suspension; the soil suspension was diluted 10 times using the 10-fold dilution method. -2 10 -3 10 -4 10 -5 10 -6 ;

[0037] 2. Purify the strain: Take 10 -4 10 -5 10 -6 100 μL of each dilution gradient suspension was evenly spread onto Alexandrov agar plates (5 g / L sucrose, 2 g / L Na2HPO4, 0.5 g / L MgSO4·7H2O, 0.005 g / L FeCl3·6H2O, 0.1 g / L CaCO3, 2.5 g / L magnesium silicate, 20 g / L agar) and incubated at 30°C for 2–3 days. Single colonies with characteristics of silica-dissolving microorganisms were picked, purified, and streaked on Alexandrov agar plates. This process was repeated 2–3 times.

[0038] 3. Strain identification

[0039] (1) Gram staining

[0040] The single strain was screened by dilution plating method and streaked on LB agar plates (NaCl 10 g / L, peptone 10 g / L, yeast extract 5 g / L, agar 1.5~2.0 g / L), and incubated at 30℃ for 24 h to observe colony morphology. The bacterial culture was inoculated into liquid LB medium (NaCl 10 g / L, peptone 10 g / L, yeast extract 5 g / L). 10 μL of the bacterial culture was dropped onto a sterile glass slide and the strain was fixed by gently heating it over an alcohol lamp flame. After the slide cooled, crystal violet solution was added until it covered the colonies, and the staining was performed for about 1 minute. The stain was then gently rinsed off under tap water and dried with absorbent paper. Iodine solution was added until it covered the colonies, and the staining was performed for about 1 minute. The stain was rinsed off in the same manner and dried. 95% anhydrous ethanol was added to decolorize the colonies until they were colorless, and the slide was washed with water. Tomato red stain was added until it covered the colonies, and the staining was performed for about 1 minute. The slide was then rinsed off in the same manner and dried. The strain was then observed under a microscope.

[0041] The results are as follows Figure 1 As shown: The colonies of strain psm006 on LB solid plates are white, smooth, opaque, with neat edges, a raised center, and a diameter of 1 mm. Gram staining results are purple-red, indicating that it is a Gram-negative bacterium. Under a microscope, the cells of psm006 are observed to be rod-shaped or slightly curved.

[0042] (2) Molecular biological identification

[0043] The genome of the bacterial strain was extracted using a bacterial DNA extraction kit (Sangon Biotech (Shanghai) Co., Ltd.). Using this genome as a template, the selected strain was amplified by PCR using the universal 16S rDNA primer sequences 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-GGTTACCTTGTTACGACTT-3') (the amplification system is shown in Table 1). After completion, the strain was sent to Shanghai JEL Biotechnology Co., Ltd. for sequencing. The sequencing results are shown in SEQ ID No. 1.

[0044] Table 1 PCR system

[0045]

[0046] The obtained sequences were submitted to NCBI for BLAST sequence alignment, and a phylogenetic tree was constructed using mega11 software (Molecular Evolutionary Genetics Analysis).

[0047] The results show that:

[0048] like Figure 2As shown, the strain can be identified as belonging to the genus *Pseudomonas* through its phylogenetic tree, and therefore named *Pseudomonas montelukastii*. Pseudomonas monteilii ).

[0049] Example 2

[0050] The silicon solubility of Pseudomonas montmorillonite psm006 obtained from Example 1 was tested. The specific process is as follows:

[0051] (1) Qualitative testing of plate form:

[0052] Take 10 μL of bacterial culture and place it on a magnesium silicate plate (10 g / L glucose, 2.5 g / L magnesium silicate, 1.5 g / L agar). Observe the size of the hydrolysis zone. The results are as follows: Figure 3 As shown, the strain can produce transparent hydrolysis zones on the plate, indicating that it has the ability to dissolve silicon.

[0053] (2) Quantitative detection using liquid method:

[0054] Inoculate 1% of the culture medium into Alexandrov broth and incubate at 30°C and 200 rpm for 5 days. Centrifuge the culture at 8000 rpm for 15 min, collect the supernatant, filter it, and analyze the soluble silicon content in the supernatant using ICP-MS (Inductively Coupled Plasma-Mass Spectrometry). Results are as follows. Figure 4 As shown, compared with the blank control group CK without the addition of the strain, the soluble silicon content in the culture supernatant after the strain was added was significantly increased, reaching a statistically significant difference.

[0055] Example 3

[0056] Detection of salt and alkali tolerance and growth-promoting ability of Pseudomonas montelukastii psm006

[0057] (1) Salt tolerance test

[0058] Single colonies were picked and incubated overnight in LB liquid medium. They were then inoculated at a 1% inoculum into LB liquid medium containing 1%, 3%, 5%, 6%, 7%, 8%, 9%, 10%, and 11% NaCl (w / v), respectively, and cultured in a shaker at 30°C for 24 hours. The OD600 of the bacterial culture was measured using a spectrophotometer.

[0059] The results are as follows Figure 5 As shown, the OD600 of the strain gradually decreased with increasing NaCl concentration. Taking 6% NaCl as the critical value, the growth of the strain was inhibited when the NaCl concentration was 6% (w / v), but a relatively large number of cells could still grow. When the NaCl concentration was greater than 6% (w / v), the OD600 dropped sharply to below 1, indicating that the growth of the strain was greatly inhibited, suggesting that the strain can tolerate a maximum NaCl concentration of 6%.

[0060] (2) Alkali resistance test

[0061] Single colonies were picked and incubated overnight in LB liquid medium. Inoculation was then performed at 1% inoculum in LB liquid medium at pH values ​​of 7, 8, 9, 10, and 11, respectively. The cultures were incubated at 30°C in a shaker for 24 hours. The OD values ​​of the bacterial cultures were measured using a spectrophotometer. 600 .

[0062] The results are as follows Figure 6 As shown, the strain can grow and reproduce well at pH ≤ 9. When the pH is greater than 9, the OD600 drops to almost 0, indicating that the strain can tolerate an alkaline environment up to pH 9.

[0063] (3) Determination of auxin (IAA)

[0064] Single colonies were picked and incubated overnight in LB liquid medium. A 1% inoculum was then added to LB liquid medium containing L-tryptophan (200 mg / L) and cultured in a shaker at 180 r / min and 30℃ for 36 h. Two mL of the bacterial culture was transferred to a centrifuge tube and centrifuged at 10000 r / min for 10 min. 1.5 mL of the supernatant was collected and an equal volume of Salkowski colorimetric solution (50 mL 35% HClO4 + 1 mL 0.5 mol / L FeCl3) was added to a 5 mL centrifuge tube. The mixture was incubated in the dark for 30 min, and the OD530 value was measured. A standard solution of analytical grade indoleacetic acid was prepared, and the Salkowski colorimetric reaction was performed to obtain a standard curve. The IAA content per unit volume of fermentation broth was calculated by referring to the standard curve.

[0065] The results showed that this strain (Pseudomonas montelukastii psm006) could produce 11.5±0.46 mg / L indoleacetic acid, compared to only 8.0 mg / L in the negative control Escherichia coli DH5-α.

[0066] (4) Determination of ferrocarriers

[0067] Single colonies were picked and incubated overnight in LB liquid medium; 1% inoculum was then inoculated into MKB liquid medium and incubated at 30°C in a shaker for 36 h; 1 mL of bacterial culture was centrifuged at 12000 rpm for 10 min, and 100 μL of the supernatant was mixed with 100 μL of CAS quantitative detection solution. The same procedure was performed under sterile ddH2O as a control. After reacting at room temperature for 1 h, the OD630 values ​​of the sample (As) and control (Ar) were recorded respectively. The siderophore yield was calculated using the following formula:

[0068] Ferric carrier unit (%) = 100 × (Ar - As) / Ar

[0069] The results showed that the siderophore production of this strain (Pseudomonas montelukastii psm006) was 67.9±1.02%, compared with only 14.9% in the negative control Escherichia coli DH5-α.

[0070] (5) Assay for ACC deaminase production capacity

[0071] a. Pick a single colony and place it in 30 mL of DF (KH2PO4 4 g / L, Na2HPO4 6 g / L, MgSO4·7H2O 0.2 g / L, glucose 2 g / L, gluconic acid 2 g / L, citric acid 2 g / L, H3BO3 0.01 mg / L, MnSO4·H2O 0.01 mg / L, ZnSO4·7H2O 0.078 mg / L, CuSO4·5H2O 0.05 mg / L, MoO3 0.01 mg / L, FeSO4·7H2O 1 mg / L) liquid medium and incubate at 30 ℃ and 200 r / min in the dark with shaking for 12 h.

[0072] b. Centrifuge the bacterial suspension at 4 ℃ and 8000 r / min for 10 min, collect the bacterial suspension, and resuspend the precipitate in 30 mL of ADF (DF medium with 5 mmol / L ACC solution) liquid medium, and culture in the dark with shaking at 30 ℃ and 200 r / min for 24 h.

[0073] c. Centrifuge again at 4 ℃ and 8000 r / min for 10 min, resuspend in 5 mL of 0.1 mol / L Tris-HCl buffer (pH 7.6), wash twice; transfer to a 1.5 mL centrifuge tube, resuspend the bacterial cells in 600 μL of 0.1 mol / L Tris-HCl buffer (pH 8.5) to dissolve the bacterial cells; add 30 μL of toluene and vortex for 30 s to disrupt the bacterial cells;

[0074] d. Enzyme activity assay: Take 200 μL of the lysed cell suspension, add 20 μL of 0.5 mol / L ACC solution, mix well, and heat in a water bath at 30 ℃ for 15 min;

[0075] e. Then add 1 mL of 0.56 mol / L HCl solution and shake well, then centrifuge at 10000 r / min for 10 min;

[0076] f. Take 1 mL of the above centrifuged supernatant, add 800 μL of 0.56 mol / L HCl solution, mix well, then add 300 μL of 2 g / L 2,4-dinitrophenylhydrazine solution, and incubate in a water bath at 30 ℃ for 30 min.

[0077] g. Add 2 mL of 2 mol / L NaOH solution for color development and detect the OD540 value.

[0078] After deducting spontaneous products from the control sample, the OD540 value of each strain was used to calculate the corresponding α-butanone content. The amount of 1 μmol / L α-butanone formed per minute was defined as 1 enzyme activity unit.

[0079] (6) Measurement of cell protein concentration

[0080] Take 10 μL of toluene-modified bacterial cells and dilute 10-fold with 1×PBS; dilute 1 mg / mL BSA protein standard solution with 1×PBS to obtain 20 μg / mL. -1 40 μg·mL -1 60 μg·mL -1 80 μg·mL -1 100 μg·mL -1 Protein concentration gradients were established, with 1×PBS as a blank control, and each concentration was replicated in triplicate. Protein concentrations were measured using the Bradford Protein Kit, and the OD595 value of each well was measured using a microplate reader after 5 min of reaction. A standard curve was plotted with the average OD595 value as the ordinate and the corresponding protein concentration as the abscissa. The protein concentration of the sample dilution was determined on the standard curve based on the average OD595 value of the sample dilution.

[0081]

[0082] The results showed that psm006 could produce ACC deaminase with an enzyme activity of 1.17 ± 0.35 U / mg. With Escherichia coli DH5-α as a negative control, the enzyme activity produced was 0.0073 U / mg.

[0083] (7) Determination of phosphorus solubility

[0084] Take 10 μL of bacterial culture and incubate it in Monkina Inorganic Phosphorus Medium (PKO) (10 g / L glucose, 0.5 g / L (NH4)2SO4, 0.3 g / L KCl, 0.3 g / L NaCl, 0.03 g / L FeSO4·7H2O, 0.3 g / L MgSO4·7H2O, 0.03 g / L MnSO4·4H2O, 10 g / L Ca3(PO4)2, 15 g / L agar) at 30°C for 5-7 days. If a transparent hydrolysis zone is produced, it indicates that the bacteria have phosphorus-solubilizing ability.

[0085] The results are as follows Figure 7As shown: On PKO plates, transparent hydrolysis zones appeared around the colonies, indicating that the strain has phosphate-solubilizing ability.

[0086] (8) Nitrogen fixation capacity determination

[0087] Take 10 μL of bacterial culture and place it on Ashby nitrogen-free medium plates (KH2PO4 0.2 g / L, NaCl 0.2 g / L, MgSO4 0.2 g / L, CaCO3 5 g / L, CaSO4 0.1 g / L, glucose 10 g / L, agar powder 20 g / L) and incubate at 30 ℃ for 5-7 days. If a transparent hydrolysis zone is produced, it indicates that the bacteria have nitrogen-fixing ability.

[0088] The results are as follows Figure 8 As shown: On Ashby nitrogen-free medium plates, transparent hydrolysis zones appeared around the colonies, indicating that the strain has nitrogen-fixing ability.

[0089] Example 4

[0090] Verify the silica-dissolving ability of Pseudomonas montelukastii psm006 under salt conditions.

[0091] Take 10 μL of bacterial culture on magnesium silicate plates under different conditions and observe the size of the hydrolysis zone. Two gradients of NaCl were designed for the experiment: (1) 3% NaCl (w / v) was added to magnesium silicate medium; (2) 6% NaCl (w / v) was added to magnesium silicate medium.

[0092] Experimental results are as follows Figure 9 As shown in the results, the strain still exhibits a certain degree of silica-dissolving ability on both plates with additional NaCl (a being 3% NaCl and b being 6% NaCl), indicating that this strain has the potential to be applied to the improvement of saline-alkali land.

[0093] Example 5

[0094] Verification of Pseudomonas montmorilloni psm006 in improving crop germination rate

[0095] 1. Effects of psm006 on the germination of Chinese cabbage seeds

[0096] (1) Inoculate the strain with strong growth-promoting ability into Alexander liquid medium, incubate at 30 °C for 24 h, and then centrifuge at 5000 rpm for 10 min;

[0097] (2) The bacterial cells were resuspended in sodium chloride of two different concentrations to make their OD values ​​the same, and a suspension was obtained.

[0098] Six treatments were designed: (1) CK group: tap water was used as a blank control; (2) 3 g / L NaCl without bacteria; (3) 6 g / L NaCl without bacteria; (4) additional strain psm006 was added; (5) psm006 was added to 3 g / L NaCl; (6) psm006 was added to 6 g / L NaCl.

[0099] The filter paper was immersed in the suspension and liquid of the above different treatments, and then the surface-sterilized cabbage seeds and filter paper were placed together in a petri dish to form a "sandwich" structure and placed in a suitable environment for 3-5 days.

[0100] (3) Observe the seed germination.

[0101] The results are as follows Figure 10 As shown, salt stress inhibits the germination of Chinese cabbage seeds, and the higher the salt concentration, the more obvious the inhibitory effect. However, after inoculation with the psm006 strain, it can promote the germination of Chinese cabbage under salt stress and alleviate the damage caused by salt stress.

[0102] Example 6

[0103] Antibacterial assay of Pseudomonas montelukastii psm006

[0104] Use a sterile toothpick to pick up a 2×2cm fungal block and place it upside down in the center of a PDA solid plate. Add an equal amount of psm006 bacterial solution with the same OD to the three corners of the plate. Incubate at 26 ℃ for 5 days and then observe the antibacterial results.

[0105] The result is as follows Figure 11 As shown, and by Figure 11 It can be seen that strain psm006 is effective against the pathogen of maize stalk rot: Fusarium moniliforme (… Fusarium verticillioides ) and the pathogen of wheat take-all disease: — *Oat vulgaris* ( Gaeumonnomyces graminis var avenae All of them have a certain antagonistic effect and can inhibit the growth and reproduction of certain pathogens, indicating that the strain has potential biocontrol capabilities.

[0106] The embodiments of this application have been described above with reference to the accompanying drawings. Specific examples have been used to illustrate the principles and implementation methods of this application. The description of the above embodiments is only for the purpose of helping to understand the core ideas of this application. However, this application is not limited to the specific embodiments described above. The specific embodiments described above are merely illustrative and not restrictive. Those skilled in the art can make many other forms under the guidance of this application without departing from the spirit and scope of the claims, and all of these forms are within the protection scope of this application.

Claims

1. A strain of *Pseudomonas montelukastii* psm006, characterized in that, The Pseudomonas montelukastii ( Pseudomonas monteilii psm006 was deposited on January 3, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC NO. 33294.

2. The use of Pseudomonas montmorillonii psm006 or its bacterial suspension as described in claim 1 in promoting the germination of Chinese cabbage seeds and / or in the preparation of a Chinese cabbage seed germination promoter.

3. A germination promoter for Chinese cabbage seeds, characterized in that, The cabbage seed germination promoter includes Pseudomonas montmorillonii psm006 as described in claim 1.

4. The use of Pseudomonas montmorillonii psm006 or its bacterial culture as described in claim 1 in the degradation of silica and / or the preparation of silica degrading agents.

5. A silicate degrading agent, characterized in that, The silicate degrading agent includes Pseudomonas montelukastii psm006 as described in claim 1.

6. The use of *Pseudomonas montmorillonii* psm006 or its bacterial suspension as described in claim 1 in inhibiting plant pathogens and / or preparing plant pathogen inhibitors, characterized in that... The plant pathogens are Fusarium moniliforme and Oat varietalus.

7. A plant pathogen inhibitor, characterized in that, The plant pathogen inhibitor includes Pseudomonas montmorillonii psm006 or its bacterial suspension as described in claim 1.

8. A method for inhibiting plant pathogens, characterized in that, Plants were treated with Pseudomonas montmorillonii psm006 or its bacterial solution as described in claim 1, wherein the plant pathogens were Fusarium moniliforme and Oat varietalus.

Images