Composition comprising sophorolipid and lactic acid

Abstract

The present invention relates to a composition comprising a concentration of sophorolipid and lactic acid or a salt thereof.

Description

Field of the Invention

[0001] The present invention relates to a composition comprising a certain concentration of sophorolipid and lactic acid or its salt. Prior Art

[0002] US2022064577 discloses a cleaning composition comprising at least one pH regulator and at least one biosurfactant selected from glycolipids, lipopeptides, and mixtures thereof, wherein the composition has a pH of from about 1 to about 14.

[0003] One drawback of the disclosed system is that the concentration of its biosurfactant is very low, and thus it has no practical impact on the sustainability situation when included in the formulation. Another drawback is the use of a racemic solution of lactic acid, so it cannot ensure the exclusion of D-lactic acid in the solution, which should be avoided. It is not a highly toxic compound, but is recognized as a significant marker and toxic metabolite that can cause health problems (Pohanka M. D-Lactic Acid as a Metabolite: Toxicology, Diagnosis, and Detection. Biomed Res Int. June 17, 2020; 2020).

[0004] IN202011005229 discloses different cleaning bath preparations for pets, which contain rhamnolipid as a surfactant. Lactic acid is mentioned as a pH regulator. It is also described that the bath product itself exhibits antimicrobial properties. One drawback is that the antimicrobial activity of the biosurfactant may potentially imbalance the skin microbiome. Another drawback is the use of a racemic solution of lactic acid, so it cannot ensure the exclusion of D-lactic acid in the solution, which should be avoided. It is not a highly toxic compound, but is recognized as a significant marker and toxic metabolite that can cause health problems (Pohanka M. D-Lactic Acid as a Metabolite: Toxicology, Diagnosis, and Detection. Biomed Res Int. June 17, 2020; 2020).

[0005] Silveira, V.A.I., Kobayashi, R.K.T., de Oliveira Junior, A.G. et al. evaluated the antibacterial effect of the combination of sophorolipid and lactic acid on certain bacteria in an ethanol-based solution (Braz. J. Microbiol. 2021, 52, 1769–1778). As a drawback, the addition of ethanol can be listed, because its nature as a penetration enhancer can cause skin irritation and skin dryness. Another drawback is that ethanol can affect viscosity and is the reason for the low viscosity of the preparation. Another drawback regarding detergency is the determined lactone C18:1 diacetylated form, which is lower than the corresponding acid form. This patent lists the ratio of lactic acid and sophorolipid and the lactic acid used is 10 times that of sophorolipid, which can also be regarded as a drawback because it can affect the pKs value of the preparation. Another drawback is the use of a racemic solution of lactic acid, so it cannot be ensured that D-lactic acid is excluded from the solution, which should be avoided. It is not a highly toxic compound, but is recognized as a significant marker and toxic metabolite that can cause health problems (Pohanka M. D-Lactic Acid as a Metabolite: Toxicology, Diagnosis, and Detection. Biomed Res Int. June 17, 2020; 2020).

[0006] The team of I.M. Banat (FEMS Microbiology Letters. 2016, 363, fnv224) demonstrated that biosurfactants can have antibacterial properties against certain Gram-positive and Gram-negative bacteria. As an example, the combination of 1% sophorolipid and 0.8% lactic acid is listed. A drawback of this application is that the ratio of lactic acid to biosurfactant is relatively high, which may lead to an increased potential for skin irritation. These high concentrations of biosurfactants and antimicrobial activity may potentially imbalance the skin microbiome and cause inflammatory skin conditions. Another drawback is the use of a racemic solution of lactic acid, so it cannot be ensured that D-lactic acid is excluded from the solution, which should be avoided. It is not a highly toxic compound, but is recognized as a significant marker and toxic metabolite that can cause health problems (Pohanka M. D-Lactic Acid as a Metabolite: Toxicology, Diagnosis, and Detection. Biomed Res Int. June 17, 2020; 2020).

[0007] An object of the present invention is to provide a composition having excellent cleaning properties, especially for the skin surface.

[0008] Description of the Invention

[0009] Surprisingly, it has been found that the combination of sophorolipids and lactic acid solves the problems of the present invention.

[0010] The present invention thus provides compositions comprising a certain concentration of sophorolipids and lactic acid or its salts and their uses.

[0011] One advantage of the compositions according to the present invention is that their foaming is reduced, which is desirable in, for example, eye makeup removal applications and is particularly advantageous in cleaning processes carried out by machines to avoid excessive foam leakage.

[0012] A further advantage is the reduced need for microbiological active preservatives.

[0013] A further advantage of the compositions according to the present invention is their low viscosity and thus their simple processability in any desired aqueous surfactant system.

[0014] A further advantage of the compositions according to the present invention is their good skin and hair cleaning properties.

[0015] A further advantage of the compositions according to the present invention is their good makeup removal properties.

[0016] A further advantage of the compositions according to the present invention is their good lip balm removal properties.

[0017] A further advantage of the compositions according to the present invention is their good eye makeup removal properties.

[0018] A further advantage of the compositions according to the present invention is their excellent solubilization efficacy for essential oils at low dosages.

[0019] A further advantage of the compositions according to the present invention is their mildness and good physiological compatibility.

[0020] A further advantage of the compositions according to the present invention is their good skin feel during and after washing.

[0021] A further advantage of the compositions according to the present invention is their good rinsability compared to other surfactant compositions.

[0022] A further advantage of the compositions according to the present invention is that they leave a smooth and soft skin feel after washing.

[0023] A further advantage of the mixture compositions according to the present invention is that they can be synthesized without petrochemical-based raw materials.

[0024] A further advantage of the mixture compositions according to the present invention is that they can be synthesized without key raw materials such as tropical oils.

[0025] A further advantage of the mixture compositions according to the invention is their excellent microbial stability.

[0026] A further advantage is that they can contain the L-lactic acid enantiomer, which can be obtained only by fermentation and is thus a sustainable natural raw material.

[0027] A further advantage is that when preserved with lactic acid, the acidic sophorolipids exhibit improved makeup removing properties.

[0028] A further advantage of the compositions according to the invention is their excellent properties as emulsifiers, especially when the sophorolipids contain a high content of the lactone form.

[0029] A further advantage of the compositions according to the invention is their excellent properties as solubilizers, especially when the sophorolipids contain a high content of the lactone form.

[0030] A further advantage of the compositions according to the invention is their anti-acne activity, especially when the sophorolipids contain a high content of the lactone form.

[0031] A further advantage of the compositions according to the invention is their positive effect on the microbiome, such as on the skin or textile surfaces, especially when the sophorolipids contain a high content of the lactone form.

[0032] The invention includes a composition comprising

[0033] a) at least one sophorolipid

[0034] b) lactic acid and / or its salts,

[0035] characterized in that

[0036] the content of a) is from 1.2% to 70.0% by weight and

[0037] the content of b) is from 0.08% to 10.0% by weight,

[0038] where the weight percentages are based on the total composition.

[0039] Unless otherwise stated, the percentages are data expressed as weight percentages.

[0040] Whenever measured values are stated hereinafter, unless otherwise stated, these are determined at a temperature of 25 °C and a pressure of 1013 mbar.

[0041] Unless otherwise stated, the "pH" with respect to the present invention is defined as the value measured for the relevant composition after stirring for 5 minutes at 25 °C using a pH electrode calibrated according to ISO 4319 (1977).

[0042] In the context of the present invention, the term "sophorolipid" is preferably understood to refer to compounds of the general formulas (Ia) and (Ib) and their salts

[0043]

[0044]

[0045] wherein

[0046] R 1SL = H or CO-CH 3 ,

[0047] R 2SL = H or CO-CH 3 ,

[0048] R 3SL = a divalent organic moiety containing 6 to 32 carbon atoms and which is unsubstituted or hydroxy-functionally substituted, unbranched and optionally contains 1 to 3 double or triple bonds,

[0049] R 4SL = H, CH 3 or a monovalent organic group containing 2 to 10 carbon atoms and which is unsubstituted or hydroxy-functionally substituted, unbranched and optionally contains 1 to 3 double or triple bonds, and

[0050] nSL = 1 or 0.

[0051] The sophorolipids can be used according to the invention in their acid form or their lactone form.

[0052] The preferred compositions according to the invention are characterized in that component a) has a lactone-type sophorolipid content of more than 50% by weight, preferably more than 80% by weight, more preferably more than 95% by weight, where the weight percentages are based on the total of component a).

[0053] For the determination of the content of sophorolipids in acid or lactone form in the composition, reference is made to EP1411111B1, page 8, paragraph

[0053] .

[0054] The alternative preferred compositions according to the invention are characterized in that component a) has an acid-type sophorolipid content of 55% to 99% by weight, preferably 58% to 98% by weight, more preferably 82% to 95% by weight.

[0055] A preferred composition according to the invention is characterized in that the total content of monohydric alcohols selected from methanol, ethanol, propanol, isopropanol and butanol is from 0.0% to 10.0% by weight, preferably from 0.0% to 2.0% by weight,

[0056] where the weight percentages are based on the total composition.

[0057] A preferred composition according to the invention is characterized in that it comprises water, preferably in an amount of from 25.0% to 95.0% by weight,

[0058] where the percentages by weight are based on the total composition.

[0059] A preferred composition according to the invention is characterized in that the weight ratio of component a) to component b) is from 5:1 to 30:1, preferably from 10:1 to 20:1.

[0060] The lactic acid in component b) can be included as the free acid or in the form of its salts.

[0061] The counterions in the form of the lactate are preferably selected from sodium and ammonium, preferably sodium.

[0062] The lactic acid can be included in the form of the two enantiomers D- or L-.

[0063] Mixtures as well as racemates can of course be used.

[0064] A preferred composition according to the invention is characterized in that, in component b), the L-enantiomer content is higher than 51.0% by weight, preferably higher than 80.0% by weight, more preferably higher than 95% by weight,

[0065] where the percentages by weight are based on the total of component b).

[0066] The composition according to the invention preferably has a pH of from 3.5 to 11.0, preferably from 4.0 to 9.0, particularly preferably from 4.5 to 7.0.

[0067] A preferred composition according to the invention is characterized in that

[0068] the content of a) is from 30.0% to 55.0% by weight,

[0069] the content of b) is from 1.0% to 5.0% by weight, and

[0070] the content of water is from 30.0% to 69.0% by weight,

[0071] where the percentages by weight are based on the sum of a), b) and water.

[0072] A preferred composition according to the invention is a concentrated composition which can serve as a stock composition, for example as a basic ingredient for the preparation of formulations.

[0073] Thus, the preferred composition according to any one of the preceding claims is characterized in that

[0074] the content of a) is from 30.0% to 55.0% by weight,

[0075] The content of b) is 1.0% to 5.0% by weight, and

[0076] the content of water is 40.0% to 69.0% by weight,

[0077] where the weight percentages are based on the total composition.

[0078] Of course, the composite preparations for specific uses made by combining the concentrated stock composition of the present invention with other components are also part of the present invention.

[0079] Accordingly, the present invention encompasses a preferred composition according to the present invention, preferably a cosmetic, pharmaceutical or household care preparation, characterized in that

[0080] the content of a) is 1.2% to 10.0% by weight, and

[0081] the content of b) is 0.08% to 3.0% by weight,

[0082] where the weight percentages are based on the total composition.

[0083] The composition according to the present invention can be formulated as a household care composition; preferably, in this case, the following applies:

[0084] The preparation according to the present invention can further comprise one or more auxiliaries selected from bleaching systems, hydrotropes, polymers (which may be synthetic), biopolymers, anti-redeposition aids, fiber protectants, detergents, dye transfer inhibitors, fabric toners, opacifiers, blueing dyes, enzyme stabilizers, solvents, viscosity improvers, preservatives, pH regulators and salts such as NaCl and Na 2 SO 4 .

[0085] The composition according to the present invention can be formulated as a cosmetic or pharmaceutical composition; preferably, in this case, the following applies:

[0086] The composition according to the present invention can further comprise at least one additional component selected from emollients,

[0087] emulsifiers,

[0088] thickeners / viscosity regulators / stabilizers,

[0089] ultraviolet protection filters,

[0090] antioxidants,

[0091] hydrotropes,

[0092] solids and fillers,

[0093] Film-forming agent

[0094] Pearlescent additive

[0095] Deodorant and antiperspirant active ingredient

[0096] Insect repellent

[0097] Self-tanning agent

[0098] Preservative

[0099] Conditioning agent

[0100] Fragrance

[0101] Dye

[0102] Odor absorber

[0103] Superfatting agents

[0104] Other solvents

[0105] Substances that can be used as exemplary representatives of the respective classes are known to those skilled in the art and can be found, for example, in German application DE 102008001788.4. This patent application is hereby incorporated by reference and thus constitutes part of this disclosure.

[0106] Regarding further optional components and the amounts thereof, express reference is made to the relevant manuals known to those skilled in the art, such as K. Schrader, "Grundlagen und Rezepturen der Kosmetika [Cosmetics - fundamentals and formulations]", 2nd edition, pages 329 to 341, Hüthig Buch Verlag Heidelberg.

[0107] The amount of a particular additive depends on the intended use.

[0108] Typical boundary formulations for their respective applications are known in the prior art and are included, for example, in the manufacturer's manuals of specific bases and active ingredients. These existing formulations can generally be adopted as they are. However, if necessary, for adjustment and optimization, the required modifications can be made without complication by simple experiments.

[0109] A preferred composition according to the invention comprises a further biosurfactant, which is preferably selected from rhamnolipids, glycolipids, cellulose lipids, mannitol erythritol lipids and trehalose lipids, preferably rhamnolipids and glycolipids, and most preferably rhamnolipids.

[0110] In the context of the present invention, "biosurfactant" is understood to mean all glycolipids produced by fermentation. The term "biosurfactant" also encompasses glycolipids that have been chemically or enzymatically modified after fermentation, provided that the glycolipid structure is retained.

[0111] Raw materials that can be used for the production of biosurfactants are carbohydrates, especially sugars such as glucose and / or lipophilic carbon sources such as fats, oils, partial glycerides, fatty acids, fatty alcohols, long-chain saturated or unsaturated hydrocarbons.

[0112] In the context of the present invention, the term "surfactant" is understood to mean an organic substance having surface-active properties that is capable of reducing the surface tension of water to below 45 mN / m at 20 °C and at a concentration of 0.5% by weight based on the total composition. The surface tension is measured at 20 °C by the Du Noüy ring method.

[0113] The term "rhamnolipid" is preferably understood in the context of the present invention to particularly refer to compounds of general formula (I) and their salts,

[0114]

[0115] where

[0116] mRL = 2, 1 or 0,

[0117] nRL = 1 or 0,

[0118] R 1RL and R 2RL = independently of one another are the same or different organic residues having 2 to 24, preferably 5 to 13 carbon atoms, especially optionally branched, optionally substituted, especially hydroxy-substituted, optionally unsaturated, especially optionally mono-, di- or tri-unsaturated alkyl residues, preferably selected from pentenyl, heptenyl, nonenyl, undecenyl and tridecenyl and those of (CH 2 ) o -CH 3 where o = 1 to 23, preferably 4 to 12.

[0119] If nRL = 1, the glycosidic bond between the two rhamnose units is preferably of the α-configuration. The optically active carbon atom of the fatty acid is preferably present as the R-enantiomer (e.g., (R)-3-{(R)-3-[2-O-(α-L-rhamnopyranosyl)-α-L-rhamnopyranosyl]oxydecanoyl}oxydecanoate).

[0120] The term "dirhamnolipid" is understood in the context of the present invention to mean a compound of general formula (I) or its salt in which nRL = 1.

[0121] The term "monorhamnolipid" is understood in the context of the present invention to mean a compound of general formula (I) in which nRL = 0 or a salt thereof.

[0122] The different rhamnolipids are abbreviated according to the following nomenclature:

[0123] "diRL-CXCY" is understood to mean a dirhamnolipid of general formula (I) in which one of the residues R 1RL and R 2RL = (CH 2 ) o -CH 3 , where o = X - 4, and the remaining residue R 1 or R 2 = (CH 2 ) o -CH 3 , where o = Y - 4.

[0124] "monoRL-CXCY" is understood to mean a monorhamnolipid of general formula (I) in which one of the residues R 1RL and R 2RL = (CH 2 ) o -CH 3 , where o = X - 4, and the remaining residue R 1RL or R 2RL = (CH 2 ) o -CH 3 , where o = Y - 4.

[0125] Thus, the nomenclature used does not distinguish between "CXCY" and "CYCX".

[0126] For rhamnolipids in which mRL = 0, monoRL-CX or diRL-CX is used accordingly.

[0127] If one of the above-mentioned labels X and / or Y bears ":Z", this means that the corresponding residue R 1RL and / or R 2RL is equal to an unbranched, unsubstituted hydrocarbon residue having X - 3 or Y - 3 carbon atoms with Z double bonds.

[0128] Methods for preparing the relevant rhamnolipids are disclosed, for example, in EP2786743 and EP2787065.

[0129] The rhamnolipids applicable to the present invention can also be produced by fermentation of the genus Pseudomonas, especially Pseudomonas aeruginosa, which are preferably non-genetically modified cells. This is a technology that was already publicly known in the 1980s, as described, for example, in EP0282942 and DE4127908. The rhamnolipids produced in Pseudomonas aeruginosa cells that have been improved by genetic modification for higher rhamnolipid titers can also be used in the present invention; for example, Lei et al. disclosed such cells in Biotechnol Lett. June 2020; 42(6):997-1002.

[0130] The rhamnolipids produced by Pseudomonas aeruginosa are commercially available from Jeneil Biotech Inc., for example, under the trade name Zonix, from Logos Technologies (technology acquired by Stepan), for example, under the trade name NatSurFac, from Biotensidion GmbH, for example, under the trade name Rhapynal, from AGAEtechnologies, for example, under the names R90, R95, R95Md, R95Dd, from Locus Bio-Energy Solutions and from Shanghai Yusheng Industry Co., Ltd., for example, under the trade name Bio-201 Glycolipids.

[0131] The present invention provides a composition preferably comprising rhamnolipids as a further biosurfactant, characterized in that it comprises

[0132] 51% to 100% by weight, preferably 60% to 95% by weight, particularly preferably 80% to 90% by weight of mono-rhamnolipids, especially those of formula (I) where nRL = 0,

[0133] where the weight percentages are based on the total of all rhamnolipids present.

[0134] The present invention further provides a composition preferably comprising rhamnolipids as a further biosurfactant, characterized in that it comprises

[0135] 51% to 95% by weight, preferably 55% to 80% by weight, particularly preferably 60% to 70% by weight of diRL-C10C10,

[0136] where the weight percentages are based on the total of all rhamnolipids present.

[0137] A preferred composition according to the present invention is characterized in that the composition comprises the rhamnolipids as described above as a further biosurfactant in an amount of

[0138] 0.5 to 15 wt%, preferably 3 to 12 wt%, particularly preferably 5 to 10 wt% of diRL-C10C12:1,

[0139] where the weight percentages are based on the total sum of all rhamnolipids present.

[0140] A further preferred composition according to the invention is characterized in that the composition comprises rhamnolipids as described above as a further biosurfactant, in an amount of

[0141] 0.5 to 25 wt%, preferably 3 to 15 wt%, particularly preferably 5 to 12 wt% of diRL-C10C12,

[0142] where the weight percentages are based on the total sum of all rhamnolipids present.

[0143] A further preferred composition according to the invention is characterized in that the composition comprises rhamnolipids as described above as a further biosurfactant, in an amount of

[0144] 0.1 to 25 wt%, preferably 2 to 10 wt%, particularly preferably 4 to 8 wt% of diRL-C8C10,

[0145] where the weight percentages are based on the total sum of all rhamnolipids present.

[0146] A still further preferred composition according to the invention is characterized in that the composition comprises rhamnolipids as described above as a further biosurfactant, in an amount of

[0147] 0.1 to 5 wt%, preferably 0.5 to 3 wt%, particularly preferably 0.5 to 2 wt% of monoRL-C8C10 and / or, preferably and

[0148] 0.1 to 5 wt%, preferably 0.5 to 3 wt%, particularly preferably 0.5 to 2 wt% of monoRL-C10C10,

[0149] where the weight percentages are based on the total sum of all rhamnolipids present.

[0150] The present invention further provides, alternatively preferably, a composition comprising rhamnolipids as a further biosurfactant, characterized in that it comprises

[0151] 10 to 50 wt%, preferably 20 to 40 wt%, particularly preferably 25 to 35 wt% of monoRL-C10C10 and

[0152] The weight percentages are based on the sum of all rhamnolipids present.

[0153] An alternative preferred composition according to the invention is preferably characterized in that the composition comprises rhamnolipids as further biosurfactants as described above, in an amount of

[0154] 10 wt% to 30 wt%, preferably 12 wt% to 25 wt%, particularly preferably 15 wt% to 20 wt% of diRL-C10C10,

[0155] The weight percentages are based on the sum of all rhamnolipids present.

[0156] An alternative preferred composition according to the invention is preferably characterized in that the composition comprises rhamnolipids as further biosurfactants as described above, in an amount of

[0157] 10 wt% to 30 wt%, preferably 12 wt% to 25 wt%, particularly preferably 15 wt% to 20 wt% of monoRL-C8C10,

[0158] The weight percentages are based on the sum of all rhamnolipids present.

[0159] An alternative preferred composition according to the invention is preferably characterized in that the composition comprises rhamnolipids as further biosurfactants as described above, in an amount of

[0160] 3 wt% to 25 wt%, preferably 5 wt% to 20 wt%, particularly preferably 10 wt% to 15 wt% of monoRL-C10C12:1,

[0161] The weight percentages are based on the sum of all rhamnolipids present.

[0162] An alternative preferred composition according to the invention is preferably characterized in that the composition comprises rhamnolipids as further biosurfactants as described above, in an amount of

[0163] 1 wt% to 15 wt%, preferably 2 wt% to 10 wt%, particularly preferably 3 wt% to 8 wt% of diRL-C10C12,

[0164] The weight percentages are based on the sum of all rhamnolipids present.

[0165] Methods for preparing the relevant rhamnolipids are disclosed, for example, in EP2786743 and EP2787065.

[0166] The rhamnolipids applicable to the present invention can also be produced by fermentation of the genus Pseudomonas, especially Pseudomonas aeruginosa, which are preferably non-genetically modified cells. This is a technology that was already disclosed in the 1980s, as described, for example, in EP0282942 and DE4127908. The rhamnolipids produced in Pseudomonas aeruginosa cells that have been improved by genetic modification for higher rhamnolipid titers can also be used in the present invention; for example, Lei et al. disclosed such cells in Biotechnol Lett. June 2020; 42(6):997-1002.

[0167] The rhamnolipids produced by Pseudomonas aeruginosa are commercially available from Jeneil Biotech Inc., for example, under the trade name Zonix, from Logos Technologies (technology acquired by Stepan), for example, under the trade name NatSurFac, from Biotensidion GmbH, for example, under the trade name Rhapynal, from AGAE technologies, for example, under the names R90, R95, R95Md, R95Dd, from Locus Bio-Energy Solutions and from Shanghai Yusheng Industry Co., Ltd., for example, under the trade name Bio-201 Glycolipids.

[0168] The compositions according to the present invention can be used in different application fields, for example, for various cleaning applications.

[0169] In the present context, the preferred compositions according to the present invention are formulations, preferably cosmetic, laundry or dishwashing formulations.

[0170] Another aspect of the present invention is the use of the compositions according to the present invention for cleaning surfaces, which are preferably selected from skin, dish surfaces or textile surfaces, most preferably skin.

[0171] Another aspect of the present invention is the use of the compositions according to the present invention for moisturizing human skin. Brief description of the drawings:

[0173] Figure 1 Graphically illustrate the foam volume of the test solution over time.

[0174] The following cited examples describe the present invention by way of illustration and are not intended to limit the present invention to the embodiments specified therein. The scope of application of the present invention is obvious from the entire specification and claims. Examples

[0175] Example 1: Preparation of the composition according to the present invention

[0176] Preparation of sophorolipids

[0177] Based on the substrates glucose, sunflower oil, rapeseed oil or olive oil (mainly containing oleic acid as the fatty acid moiety), a crude product is prepared by fermentation with the yeast Candida bombicola. The growth medium contains the following components: 10 g / L glucose (D-(+)-glucose * 1H 2 O), 7.5 g / L YNB (yeast nitrogen base), 2 g / L yeast extract. 1.1 L of the medium is autoclaved in a 2 L fermenter and inoculated with a pre-culture in the exponential growth phase from the same medium. The temperature is set at 30 °C. Air is introduced by means of the stirrer speed to keep p(O 2 ) at 30% relative saturation, but the stirrer speed is never lower than 200 rpm. During the biomass formation phase, the pH drops to 3.5 and is maintained at this level by adding NaOH. After the end of the biomass formation phase (characterized by the depletion of the glucose present, an increase in p(O 2 ) or a decrease in p(CO 2 ), the product formation phase is initiated by adding 150 g of the corresponding oil, 200 mL of a 750 g / L glucose solution and 10 mL of a 150 g / L yeast extract solution. The end of the product formation phase is marked by a renewed increase in p(O 2 ). After fermentation, the batch is autoclaved and the crude product phase sediments as a precipitate. The crude product phase is washed with water and hexane accordingly. The product phase is then extracted with ethyl acetate, and the solvent is removed under reduced pressure. This gives a substantially anhydrous product corresponding to the dry matter of the present invention.

[0178] Analysis by HPLC-MS and NMR shows that the product mainly consists of the diacetylated sophorolipid lactone form with a glycosidic linkage of fatty acids (main components: 65 - 80 wt% sophorolipid lactone relative to all sophorolipids, 1 - 16 wt% fatty acids relative to the total composition, 1 - 3 wt% glycerol relative to the total composition).

[0179] This component acts as the sophorolipid in compositions C and D (high lactone form).

[0180] A 60 wt% aqueous suspension of sophorolipids is mixed with 0.5 wt% solid NaOH pellets. The mixture is then stirred at a temperature of 50 °C for 30 minutes to obtain the deacetylated acid form of sophorolipids by hydrolysis.

[0181] The batch is then adjusted to pH 3 by adding HCl, and the product is extracted with ethyl acetate. The ethyl acetate is removed to obtain a residue, which can be ground into a powder and has a water solubility of >50 wt%.

[0182] This component acts as sophorolipid in Compositions A and B (high-acid form), where the content of the acid form is greater than 55% by weight based on all sophorolipids.

[0183] Preparation of Composition A

[0184] Dissolve sophorolipid with a high acid content in water, and add L-(+)-lactic acid with stirring to obtain a final product with a concentration of 3% L-(+)-lactic acid and 45% sophorolipid. Adjust the pH value to 5.

[0185] Preparation of Composition B not of the present invention

[0186] Dissolve sophorolipid with a high acid content in water, and add sodium benzoate to the mixture with stirring to obtain a final product with a concentration of 1% sodium benzoate and 45% sophorolipid. Adjust the pH value to 5.

[0187] Preparation of Composition C not of the present invention

[0188] Dissolve sophorolipid with a relatively high lactone form in water, and add sodium benzoate to the mixture with stirring to obtain a final product with a concentration of 1% sodium benzoate and 45% sophorolipid. Adjust the pH value to 5.

[0189] Preparation of Composition D of the present invention

[0190] Dissolve sophorolipid with a relatively high lactone form in water, and add L-(+)-lactic acid to the mixture with stirring to obtain a final product with a concentration of 3% L-(+)-lactic acid and 45% sophorolipid. Adjust the pH value to 5.

[0191] Example 2: Makeup Removing Performance

[0192] The cleaning property was determined by removing makeup from a PMMA plate using a scrub abrasion and washability device. This method is designed to test the cleaning efficacy of aqueous surfactant solutions such as micellar water or facial toners, and can be used for makeup types that can dry completely on the plate.

[0193] Procedure:

[0194] First, use the corresponding positioning device to measure the color values of the PMMA plate itself at three defined positions. As a second step, apply the cosmetic product using a rod applicator. After drying for 1 hour, measure the color values of the coated PMMA plate at the exact same three positions as before. Fix the plate in the correct position, and prepare 2.5 g of the corresponding test solution by diluting each test substance with water to 3 wt%. Apply each to a cotton pad and attach the cotton pad to the sponge of the sliding carriage. After positioning the sliding carriage, the experiment starts. Set the cycle, where 1 cycle equals 1 forward and backward movement. Speed: 10 cycles per minute, and 1 cycle covers 160 mm. After drying for 1 hour, measure the color values on the plate at the 3 positions as before, and determine the makeup removal property using the three - rule method with the ΔE value (Δbare plate / coated plate, or Δcoated plate / clean plate).

[0195] Preparation of test solution:

[0196] Dilute all the test substances in Table 1 with water to a 3 wt% solution and adjust to pH 7.

[0197] Table 1

[0198]

[0199] The results in Table 1 show that the makeup - removing performance of Composition A is better than that of Composition B, and Composition D is better than C. Among them, Composition A containing sophorolipid with a high acidic content is superior to all of these; water and the reference are the worst.

[0200] Example 3: Evaluation of foaming property using a SITA foam tester

[0201] The foaming property of surfactants and surfactant - based cleaning products is an important consumer - perceived property. Consumers associate fast flash foaming and high foam volume with efficacy and high quality. Both of these parameters can be measured using the measuring device "SITA foam tester R - 2000" from SITA Messtechnik GmbH. In this device, air is introduced into a defined volume of surfactant solution through a special rotor to generate foam. The total volume of the liquid and the resulting foam is measured over time with the aid of computer - controlled sensing technology.

[0202] Using this method, evaluate the foaming property of Composition A containing sophorolipid and lactic acid compared to Composition B containing sophorolipid and sodium benzoate.

[0203] To evaluate the foaming performance, compositions A and B were each diluted with water to a concentration of 0.5 wt% active surfactant substance. Each solution was adjusted to a total hardness of 10 °dH (German hardness) and a pH value of 6. The foaming property of 300 mL of each test solution was tested for 10 seconds at 30 °C using a constant stirring speed of 1500 rpm. A total of 8 such measurement intervals were carried out for each test solution. All samples were tested in duplicate.

[0204] Figure 1 Graphical representation of the foam volume of each test solution over time:

[0205] Measurement parameters: Temperature: 30 °C ± 0.5 °C; Sample volume / measurement: 300 mL; Concentration of test sample: 0.5 wt% in water (10 °dH (German hardness)), pH adjusted with NaOH, Stirring speed: 1500 rpm; Stirring time: 10 seconds; Number of intervals: 8; Number of repetitions: 2.

[0206] As Figure 1 seen, at a pH value of 6, the composition containing sophorolipid and lactic acid has a lower foam volume compared to composition B. This result is desirable as a low foam volume is preferred in eye makeup removers and micellar water preparations.

[0207] Shampoo example formulations:

[0208] Example formulation 1: Volume and Body Shampoo

[0209]

[0210]

[0211] Example formulation 2: Repair Shampoo

[0212]

[0213]

[0214] Example formulation 3: Anti-dandruff Shampoo

[0215]

[0216]

[0217] Example formulation 4: Strengthening Shampoo

[0218]

[0219]

[0220] Example Preparation 5: Shampoo

[0221] Ingredient ％ Water ad 100 Sodium Laureth Sulfate 9.0 Cocamidopropyl Betaine 3.0 Composition A 3.0 Glycerin 2.5 Sodium Chloride 1.7 Glycol Distearate 1.5 Panthenol 0.2 Propylene Glycol 0.3 Hydroxypropyl Guar Hydroxypropyltrimonium Chloride 0.3 Sodium Benzoate 0.7 PEG-55 Propylene Glycol Oleate 0.5 Citric Acid ad pH 5.5 Hydrolyzed Wheat Protein 0.3 Argania Spinosa Kernel Oil 0.1 Laureth-4 0.5 Potassium Sorbate 0.2 Fragrance, Dye q.s.

[0222] Example Preparation 6: Moisturizing Shampoo

[0223]

[0224]

[0225] Example Preparation 7: Shampoo

[0226]

[0227]

[0228] Example Preparation 8: Cleansing Oil Shampoo

[0229]

[0230]

[0231] Example Preparation 9: Glossy Shampoo

[0232]

[0233]

[0234] Example Preparation 10: Shampoo

[0235]

[0236]

[0237] Example Preparation 11: Reinforcing Shampoo

[0238]

[0239]

[0240] Example Preparation 12: Shampoo

[0241]

[0242]

[0243] Example Preparation 13: Fortifying Shampoo

[0244]

[0245]

[0246] Example Preparation 14: Shampoo

[0247]

[0248]

[0249] Example Preparation 15: Brunette Shampoo

[0250]

[0251]

[0252] Example Preparation 16: 2-in-1 Shampoo

[0253] Ingredient ％ Water ad 100 Sodium Laureth Sulfate 8.0 PEG-200 Hydrogenated Glyceryl Palmitoleate 3.0 Composition A 3.0 Disodium Cocoamphodiacetate 3.0 Polysorbate 20 2.5 Glycerin 2.5 Sodium Chloride 2.0 PEG-7 Glyceryl Cocoate 2.0 Sodium Benzoate 1.0 Sodium Laureth-8 Sulfate 1.0 Sodium Oleth Sulfate 1.0 Sodium Hydroxide 0.3 PPG-5 Ceteth-20 1.0 PEG-55 Propylene Glycol Oleate 0.5 Polyquaternium-10 0.2 Salicylic Acid 0.3 Maltodextrin 0.2 Propylene Glycol 0.2 Disodium Ricinoleamido MEA Sulfosuccinate 0.1 Citric Acid ad pH 5.5 Prunus Armeniaca (Apricot) Kernel Oil 0.1 Fragrance, Dye q.s.

[0254] Example Preparation 17: Shampoo

[0255]

[0256] Example Preparation 18: Foam Shampoo

[0257]

[0258]

[0259] Example Preparation 19: Anti-Dandruff Shampoo

[0260]

[0261]

[0262] Example Preparation 20: Shampoo

[0263]

[0264]

[0265] Example Preparation 21: Intensive Care Oil - Shampoo

[0266]

[0267]

[0268] Example Preparation 22: Shampoo

[0269]

[0270]

[0271] Example Preparation 23: Shampoo

[0272]

[0273]

[0274] Example Preparation 24: Shampoo

[0275] Ingredient ％ Water ad 100 Sodium Cocoyl Sulfate 6.0 Lauryl Glucoside 4.0 Glycerin 3.5 Sodium Lactate 2.5 Composition A 2.0 Stearamidopropyl Dimethylamine 1.0 Betaine 0.8 Sodium Chloride 0.9 Simmondsia Chinensis (Jojoba) Seed Oil 0.8 Sodium Cocoyl Glutamate 0.7 Disodium Cocoyl Glutamate 0.5 Xanthan Gum 0.5 Hydrated Silica 0.5 Fragrance, Dye q.s.

[0276] Example Preparation 25: Children's Shampoo

[0277]

[0278]

[0279] Example Preparation 26: Intensive Cream Shampoo

[0280]

[0281]

[0282] Example Preparation 27: Shampoo

[0283] Ingredient ％ Water ad 100 Sodium Laureth Sulfate 7.5 Cocamidopropyl Betaine 3.0 Composition A 2.5 Sodium Chloride 2.0 Sodium Xylene Sulfonate 1.7 Cocamide MEA 1.5 Sodium Citrate 1.5 Citric Acid ad pH 5.0 Dimethicone Copolyol 1.0 Cassia Gum Hydroxypropyltrimonium Chloride 0.3 Sodium Benzoate 0.9 Disodium EDTA 0.7 Panthenol 0.7 Panthenyl Ethyl Ether 0.3 Persea Gratissima (Avocado) Oil 0.2 Methylchloroisothiazolinone 0.1 Methylisothiazolinone 0.1 Fragrance, Dye q.s.

[0284] Example Preparation 28: Caffeine Shampoo

[0285] Ingredient ％ Water ad 100 Sodium Laureth Sulfate 6.0 Laureth-2 4.0 Disodium Laureth Sulfosuccinate 2.5 Sodium Lauroyl Glutamate 2.5 Composition A 2.0 Sodium Chloride 2.0 Caffeine 1.5 Panthenol 0.2 PEG-120 Methyl Glucose Dioleate 0.5 Hydrolyzed Wheat Protein 0.7 Citric Acid ad pH 5.5 Sodium Citrate 0.5 Menthol 0.7 PEG-40 Hydrogenated Castor Oil 0.4 Potassium Sorbate 0.3 Polyquaternium-7 0.4 Disodium EDTA 0.5 Sodium Benzoate 0.7 PCA Zinc 0.3 Niacinamide 0.1 Limonene 0.1 Tocopherol 0.1 Phenoxyethanol 0.5 Methylparaben 0.3 Propylparaben 0.3 Dye q.s.

[0286] Example Preparation 29: Power Grey Shampoo

[0287]

[0288]

[0289] Example Preparation 30: Shampoo

[0290]

[0291]

[0292] Example Preparation 31: Caffeine Shampoo

[0293] Ingredient ％ Water ad 100 Sodium Cocoyl Sulfate 6.0 Glycerin 5.5 Lauryl Glucoside 4.5 Cocamidopropyl Betaine 3.0 Composition A 2.5 Lauryl Sarcosine 2.0 Caffeine 1.0 Xanthan Gum 1.0 Hydroxypropyl Guar Hydroxypropyltrimonium Chloride 0.3 Alcohol 0.8 Coffea Arabica Bean Extract 0.3 Panthenyl Ethyl Ether 0.4 Panthenol 0.2 Limonene 0.1 Linalool 0.1 Dye q.s.

[0294] Example Preparation 32: Shampoo

[0295] Ingredient ％ Water ad 100 Sodium Laureth Sulfate 6.5 Cocamidopropyl Betaine 3.0 Sodium Chloride 3.0 Composition A 2.0 Panthenol 0.2 Benzophenone-4 0.9 Glycol Distearate 0.7 Laureth-4 0.8 Glycerin 0.9 Formic Acid 0.2 Decyl Glucoside 0.9 Polyquaternium-10 0.4 Niacinamide 0.1 Sodium Benzoate 0.1 Potassium Sorbate 0.2 Citric Acid at pH 5.0 Fragrance, Dye q.s.

[0296] Example Preparation 33: Shampoo

[0297]

[0298]

[0299] Example Preparation 34: Volume Shampoo

[0300]

[0301]

[0302] Example Preparation 35: Shampoo

[0303]

[0304]

[0305] Example Preparation 36: Shampoo

[0306]

[0307]

[0308] Example Preparation 37: Shampoo

[0309]

[0310]

[0311] Example Preparation 38: Shampoo

[0312]

[0313]

[0314] Example Preparation 39: Conditioning Shampoo

[0315] Ingredient ％ Water up to 100 Sodium Cocoyl Amphoacetate 5.0 Cocamidopropyl Betaine 4.0 Glycolipid 1.5 Composition A 1.5 Hydroxypropyltrimonium Chloride Starch 1.2 Isostearamide MIPA; Glyceryl Laurate 1.2 Isoamyl Laurate 1.0 Bis-(Isostearoyl / Oleoyl Isopropyl) Dimethyl Ammonium Methyl Sulfate 0.5 Citric Acid 3.0 Fragrance, Preservative, Dye q.s.

[0316] Example Preparation 40: Conditioning Shampoo

[0317]

[0318]

[0319] Example Preparation 41: Conditioning Shampoo

[0320] Ingredient ％ Water up to 100 Sodium Cocoyl Amphoacetate 5.0 Cocamidopropyl Betaine 3.0 Disodium Laureth Sulfosuccinate 3.0 Glycolipid 1.5 Composition As 1.5 Palmamidopropyl Trimethyl Ammonium Chloride 2.0 Isostearamide MIPA, Glyceryl Laurate 1.0 PEG-200 Hydrogenated Glyceryl Palmitoleate; PEG-7 Glyceryl Cocoate 1.0 Methoxy PEG / PPG-7 / 3 Aminopropyl Polydimethylsiloxane 0.5 Hydroxypropyltrimonium Chloride Starch 0.3 Polyquaternium-10 0.2 Citric Acid at pH 5.5 Fragrance, Preservative q.s.

[0321] Example Preparation 42: Conditioning Shampoo

[0322]

[0323]

[0324] Example Preparation 43: Natural Shampoo

[0325] Ingredient ％ Water up to 100 Sodium Lauryl Sulfate 9.0 Glycerin 5.0 Lauryl Glucoside 2.5 Composition A 2.5 Sodium Lactate 1.0 Betaine 1.0 Hamamelis Virginiana Leaf Extract 1.0 Denatured Alcohol 1.0 Sodium / Cocoyl Glutamate 0.8 Maris Sal (Sea Salt) 0.5 Dipotassium Glycyrrhizate 0.5 Glycerophosphocholine 0.1 Fragrance, Preservative q.s.

[0326] Example Preparation 44: Natural Shampoo

[0327]

[0328]

[0329] Example Preparation 45: Natural Shampoo

[0330]

[0331]

[0332] Example Preparation 46: Natural Shampoo

[0333]

[0334]

[0335] Example Preparation 47: Natural Shampoo

[0336] Ingredient ％ Water up to 100 Cocamidopropyl Betaine 7.0 Cetyl Trimethyl Ammonium Chloride 5.0 Decyl Glucoside 5.0 Sodium Chloride 2.0 Composition A 1.0 Sorbitan Octanoate 0.8 Cocos Nucifera (Coconut) Oil 0.8 Inulin 0.5 Benzyl Alcohol 0.2 Cetearyl Alcohol 0.2 Xanthan Gum 0.2 Sodium Cocoyl Glutamate 0.1 Fragrance 0.1 Glycerin 0.2 Argania Spinosa Kernel Oil 0.1 Titanium Dioxide 0.1 Hydroxypropyl Guar Hydroxypropyltrimonium Chloride 0.1 Lactic Acid 0.1 Hexyl Cinnamal 0.1 Sodium Hydroxide 0.1 Ascorbyl Palmitate 0.1 Tocopherol 0.1

[0337] Example Preparation 48: Natural Shampoo

[0338] Ingredient ％ Water up to 100 Glycerin 5.0 Sodium Lauryl Sulfate 5.0 Lauryl Glucoside 4.0 Composition A 4.0 Betaine 3.0 Maris Sal (Sea Salt) 2.0 Camellia Oleifera Leaf Extract 1.5 Pisum Sativum Peptide 1.5 Oryza Sativa Extract 1.0 Pisum Sativum Seed Extract 0.5 Disodium Cocoyl Glutamate 0.4 Sodium Cocoyl Glutamate 0.4 PCA Glyceryl Oleate 0.2 Alcohol 0.1 Fragrance q.s.

[0339] Example Preparation 49: Anti-Hair Loss Tonic

[0340] Ingredient ％ Water up to 100 Denatured Alcohol 50.0 PEG-40 Hydrogenated Castor Oil 2.5 Composition A 1.5 PEG-7 Glyceryl Cocoate 2.0 Dihydrosphingosine 0.1 Fragrance, Preservative q.s.

[0341] Example Preparation 50: Bath Oil

[0342]

[0343] Example Preparation 51: Shower Mousse

[0344]

[0345]

[0346] Example Preparation 52: Body Wash

[0347] Ingredient ％ Glycerin 3.5 Sodium Laureth Sulfate 3.5 Composition A 3.0 Propylene Glycol 2.0 Chondrus Crispus Extract 2.0 Pyrus Malus Juice 1.0 Vitis Vinifera Juice 0.5 Fragrance 0.2 Butylphenyl Methylpropional 0.1

[0348] Example Preparation 53: Body Wash

[0349]

[0350]

[0351] Example Preparation 54: Shower Scrub

[0352] Ingredient ％ Water up to 100 Maris Sal (Sea Salt) 15.0 Sodium Laureth Sulfate 9.0 Composition A 2.0 Sodium Cocoyl Amphoacetate 1.0 Betaine 1.0 Citrus Limon (Lemon) Peel Powder 0.5 Lactic Acid at pH 5.5 Fragrance, Dye qs.

[0353] Example Preparation 55: Cellulose Scrub

[0354]

[0355]

[0356] Example Preparation 56: Exfoliating Body Scrub

[0357] Ingredient ％ Water up to 100 Coco-Glucoside 7.0 Alcohol 6.0 Glycerin 5.0 Glycine Soja Oil 5.0 Hydrated silica 4.0 Xanthan gum 3.0 Composition A 3.0 Disodium / sodium cocoyl glutamate 1.0 Coffea Arabica (Coffee) seed powder 1.0 Cocos Nucifera (Coconut) shell powder 1.0 Citric acid ad pH 5.2 Glyceryl oleate 0.5 Fragrance 0.1 Preservatives, dyes q.s.

[0358] Example Preparation 57: Dry Shampoo

[0359] Ingredients ％ Butane, propane 53.0 Denatured ethanol 36.0 Starch aluminum octenyl succinate 3.0 Panthenol 1.5 Tocopherol 1.0 Cocos Nucifera oil 1.0 Curcuma Longa (Turmeric) root extract 0.6 Silica 0.5 Sodium bicarbonate 0.5 Charcoal powder 0.4 Hydrolyzed rice protein 0.4 Aminopropyl phenyl polytrimethylsiloxane 0.4 Benzophenone-4 0.3 Polysorbate 20 0.3 Composition A 0.3 Cyclopentasiloxane 0.2 Cyclohexasiloxane 0.2 Glycerin 0.2 Water 0.2 Fragrance q.s.

[0360] Example Preparation 58: Dry Shampoo

[0361] ％ Butane, isobutane, propane 55.0 Oryza Sativa (Rice) starch 25.0 Denatured ethanol 18.0 Fragrance 1.0 Composition A 0.5 Distearyldimethylammonium chloride 0.3 Cetyltrimethylammonium chloride 0.2

[0362] Bathing Example Preparations

[0363] Example Preparation 59: Bath Gel

[0364] Ingredients ％ Water ad 100 Sodium laureth sulfate 8.5 Cocamidopropyl betaine 3.0 Sodium chloride 2.7 Composition A 2.5 Guar gum 0.5 Polyquaternium-7 0.5 Bisabolol 0.2 Trideceth-9 0.5 Sodium sulfate 0.9 Tetrasodium edetate 0.2 Citric acid ad pH 5.5 Sodium benzoate 0.7 Benzyl alcohol 0.7 Benzyl salicylate 0.1 Geraniol 0.2 Hexyl cinnamal 0.1 Limonene 0.1 Linalool 0.1 Dyes q.s.

[0365] Example Preparation 60: Body Wash

[0366]

[0367]

[0368] Example Preparation 61: Cooling Bath Gel

[0369]

[0370]

[0371] Example Preparation 62: Bath Gel

[0372]

[0373]

[0374] Example Preparation 63: Bath Gel

[0375] Ingredients ％ Water ad 100 Sodium laureth sulfate 8.0 Cocamidopropyl betaine 3.0 Acrylate copolymer 3.0 PEG-7 glyceryl cocoate 2.7 Composition A 2.5 Sunflower seed oil 1.5 Glycerin 1.5 Glycolipids 1.5 Sodium chloride 1.0 PEG-40 hydrogenated castor oil 1.0 Disodium cocoyl glutamate 1.0 Trisodium edetate 1.0 Propylene glycol 1.0 Microcrystalline cellulose 0.9 Benzophenone-4 0.5 Sodium sulfate 0.7 Phenoxyethanol 0.7 Acacia gum Propylparaben 0.1 Methylparaben 0.1 Benzyl alcohol 0.2 Fragrance, dyes q.s.

[0376] Example Preparation 64: Body Wash

[0377]

[0378]

[0379] Example Preparation 65: Anti-Aging Body Wash

[0380]

[0381]

[0382] Example Preparation 66: Bath Gel

[0383]

[0384]

[0385] Example Preparation 67: Bath Gel

[0386]

[0387]

[0388] Example Preparation 68: Men's Bath Gel

[0389] Ingredients ％ Water ad 100 Sodium laureth sulfate 9.5 Cocamidopropyl betaine 3.0 Cocamide MEA 3.0 Composition A 3.0 Glycerin 2.5 PPG-12 2.0 Sodium chloride 2.0 Citric acid ad pH 5.5 Sodium benzoate 1.0 Phenoxyethanol 0.7 Benzyl alcohol 0.7 Butylphenyl methylpropional 0.1 Citronellol 0.1 Geraniol 0.2 Hexyl cinnamal 0.1 Limonene 0.1 Linalool 0.1 Dyes q.s.

[0390] Example Preparation 69: Moisturizing Body Lotion

[0391]

[0392]

[0393] Example Preparation 70: Body Lotion

[0394]

[0395]

[0396] Example Preparation 71: Body Wash

[0397]

[0398]

[0399] Example Preparation 72: Body Wash

[0400] Ingredients ％ Water ad 100 Glycerin 7.5 Glycine Soja Oil 3.0 Lauryl glucoside 3.0 Sodium lauryl sulfate 3.0 Composition A 2.5 Alcohol 1.5 Xanthan gum 1.5 Butyrospermum Parkii Butter 1.2 Sodium cetostearyl sulfate 1.0 Sodium cocoyl glutamate 1.0 Disodium cocoyl glutamate 1.0 Tocopherol 0.1 Sunflower seed oil 0.3 Limonene 0.1 Benzyl salicylate 0.1 Linalool 0.1 Dyes q.s.

[0401] Example Preparation 73: Body Gel

[0402]

[0403]

[0404] Example Preparation 74: Body Gel

[0405] Ingredients ％ Water ad 100 Alcohol 5.0 Coco-glucoside 5.0 Glycerin 5.0 Composition A 2.5 Xanthan gum 2.5 Disodium cocoyl glutamate 2.0 Citric acid ad pH 5.0 Citrus Aurantifolia (Lime) oil 1.0 Sodium cocoyl glutamate 1.0 Glyceryl oleate 1.0 Sodium lauroyl lactylate 0.7 Limonene 0.1 Citral 0.1 Linalool 0.1 Dyes q.s.

[0406] Example Preparation 75: Body Oil

[0407] Ingredients ％ Water ad 100 Sodium laureth sulfate 8.0 Cocamidopropyl betaine 3.0 Composition A 2.0 Acrylate copolymer 2.0 Glycolipids 2.0 Tocopheryl acetate 0.5 PEG-7 glyceryl cocoate 0.5 Disodium edetate 0.4 Benzophenone-4 0.3 PEG-40 Hydrogenated Castor Oil 0.7 Cellulose 0.7 Hydroxypropyl Methylcellulose 0.7 Sodium Chloride 0.9 Ethylhexylglycerin 0.2 Phenoxyethanol 0.7 Methylisothiazolinone 0.3 Sodium Benzoate 0.3 Sodium Hydroxide 0.3 Citric Acid ad pH 5.5 Fragrance, Dye q.s.

[0408] Example Preparation 76: Body Gel

[0409]

[0410]

[0411] Example Preparation 77: Shower Gel

[0412] Ingredient ％ Water ad 100 Composition A 7.0 Sesamum Indicum (Sesame) Seed Oil 4.0 Glycolipid 3.0 Sucrose. Alcohol 2.0 Amino Acid 1.0 Glycerin 1.0 Carrageenan 0.3 Lavandula Officinalis Flower Oil 0.3 Xanthan Gum 0.3 Lactic Acid 0.1

[0413] Example Preparation 78: Shower Scrub

[0414] Ingredient ％ Water ad 100 Maris Sal (Sea Salt) 15.0 Sodium Laureth Sulfate 9.0 Composition A 2.0 Sodium Cocoyl Amphoacetate 1.0 Betaine 1.0 Citrus Limon (Lemon) Peel Powder 0.5 Lactic Acid ad pH 5.5 Fragrance, Dye qs.

[0415] Example Preparation 79: Cellulose Scrub

[0416] Ingredient ％ Water ad 100 Composition A 7.0 Cocoglucoside 6.0 Glycerin 5.0 Glyceryl Stearate 5.0 Citrus Aurantium Dulcis Fruit Water 4.5 Helianthus Annuus Hybrid Oil 4.0 Cellulose 2.0 Jojoba Esters 2.0 Cetyl Alcohol 2.0 Simmondsia Chinensis (Jojoba) Seed Oil 2.0 Olea europaea (Olive) Fruit Oil 2.0 Hydrogenated Castor Oil 0.8 Prunus Armeniaca (Apricot) Kernel Oil 0.5 Leontopodium alpinum Extract 0.1 Xanthan Gum 0.3 Benzyl Alcohol 0.2 Fragrance 0.1 Dehydroacetic Acid 0.1 Citric Acid ad pH 5.0 Lactic Acid 0.1 Sodium Hydroxide 0.1 Fragrance, Dye q.s.

[0417] Example Preparation 80: Exfoliating Body Scrub

[0418]

[0419]

[0420] Example Preparation 81: Washing Powder

[0421] Ingredient ％ Talc 60.0 Sodium Cocoamphohydroxypropylsulfonate 15.0 Sodium C14-16 Olefin Sulfonate 10.0 Sodium Lauroyl Glutamate 4.0 Composition A 3.0 Potassium Laurate 2.0 Sodium Myristoyl Glutamate 1.5 Chondrus Crispus 1.5 Isostearyl Alcohol 1.0 Glycerin 1.0 BHT 0.4 Butylene Glycol 0.3 Protease 0.2 Salicylic Acid 0.1

[0422] Example Preparation 82: Washing Powder

[0423] Ingredient ％ Hydrated Silica ad 100 Corn Starch 30.0 Sodium Cocoamphohydroxypropylsulfonate 6.0 Amylopektin 5.0 Sodium Dodecyl Sulfate 2.5 Sodium Bicarbonate 2.0 Citric Acid 1.5 Sodium Lauroyl Glutamate 1.0 Sodium Palmitate 1.0 Composition A 1.0 Diglycerin 0.9 Sodium Lauroyl Aspartate 0.8 Titanium Dioxide 0.5 Maltodextrin 0.5 Allantoin 0.4 Water 0.3 Butylene Glycol 0.3 Aloe Barbadensis Leaf Extract 0.1 Citrus Limon (Lemon) Peel Powder 0,1 Fragrance, Preservative, Dye q.s.

[0424] Example Preparation 83: Powdered Detergent

[0425]

[0426]

[0427] Example Preparation 84: Powdered Detergent

[0428] Ingredient ％ Talc ad 100 Distarch Phosphate 10.0 Disodium Laureth Sulfosuccinate 8.0 Magnesium Aluminum Silicate 5.0 Oryza Sativa Powder 5.0 Sodium Lauroyl Glutamate 2.0 Composition A 2.0 Glucose 2.0 Magnesium Oxide 2.0 Maltodextrin 2.0 Titanium Dioxide 1.0 Pumice 0.5 Chlorella Powder 0.1

[0429] Soap Example Preparation

[0430] Example Preparation 85: Mild Liquid Soap

[0431]

[0432]

[0433] Example Preparation 86: Soap

[0434]

[0435]

[0436] Example Preparation 87: Care Liquid Soap

[0437] Ingredient ％ Water ad 100 Aloe Barbadensis Leaf Juice 5.0 Ammonium Lauryl Sulfate 5.0 Cocoglucoside 4.0 Cocamidopropyl Betaine 4.0 Sodium Cocoyl Amphoacetate 1.5 Composition A 1.5 Glycerin 1.5 Sodium Chloride 1.5 Citric Acid ad pH 5.5 Sodium Benzoate 1.0 Sodium Salicylate 0.5 Methylisothiazolinone 0.3 Linalool 0.1 Butylphenyl Methylpropional 0.2 Limonene 0.1 Dye q.s.

[0438] Example Preparation 88: Cream Soap

[0439] Ingredient ％ Water ad 100 Alcohol 5.0 Cocoglucoside 5.0 Glycerin 5.0 Composition A 2.5 Disodium Cocooyl Glutamate 2.0 Xanthan Gum 1.5 Citric Acid at pH 5.5 Malva Sylvestris Leaf Extract 1.0 Sodium Lauroyl Lactylate 1.0 Glycerol Oleate 1.0 Sodium Cocoyl Glutamate 0.7 Linalool 0.1 Limonene 0.1 Dye q.s.

[0440] Example Preparation 89: Liquid Soap

[0441]

[0442]

[0443] Example Preparation 90: Liquid Soap

[0444] Ingredients ％ Water to 100 Glycerin 7.0 Alcohol 4.0 Sodium Lauryl Sulfate 3.0 Lauryl Glucoside 2.5 Composition A 2.0 Xanthan Gum 1.5 Mangifera Indica (Mango) Fruit Extract 0.7 Limonene 0.1 Linalool 0.1 Dye q.s.

[0445] Example Preparation 91: Liquid Soap

[0446]

[0447]

[0448] Example Preparation 92: Cream Soap

[0449]

[0450]

[0451] Example Preparation 93: Jelly Soap

[0452]

[0453] Example Preparation 94: Soap

[0454]

[0455]

[0456] Example Preparation 95: Soap

[0457] Ingredients ％ Water to 100 Composition A 6.0 Glycolipid 5.0 Propylene Glycol 5.0 Sunflower Seed Oil 4.0 Cocos Nucifera Oil 4.0 Titanium Dioxide 0.2 Sodium Hydroxide 0.2 Sodium Lauryl Sulfate 0.2 Fragrance 0.1 Sodium Chloride 0.1 L-Limonene 0.1

[0458] Example Preparation 96: Painting Soap

[0459]

[0460]

[0461] Example Preparation 97: Painting Soap

[0462]

[0463]

[0464] Immersion Bath Example Preparation

[0465] Example Preparation 98: Bath

[0466] Ingredients ％ Water to 100 PEG - 80 Sorbitan Laurate 4.0 Cocamidopropyl Betaine 4.0 Sodium Laureth Sulfate 4.0 Composition A 2.0 Polysorbate 20 2.0 Sodium Chloride 1.5 Citric Acid at pH 5.0 Sodium Lauroamphoacetate 1.0 PEG - 150 Distearate 1.0 Disodium Lauroamphodiacetate 1.0 Sodium Benzoate 0.5 Fragrance, Dye q.s.

[0467] Example Preparation 99: Creme Oil Bath

[0468]

[0469]

[0470] Example Preparation 100: Relaxing Good Sleep Bath

[0471]

[0472]

[0473] Example Preparation 51: Pampering Oil Bath

[0474]

[0475] Example Preparation 101: Aroma Bath

[0476]

[0477]

[0478] Example Preparation 102: Bath Bomb

[0479] Ingredients ％ Sodium Bicarbonate 70.0 Citric Acid 8.0 Citrus Nobilis Oil 6.0 Potassium Tartrate 6.0 Sodium Laureth Sulfate 4.0 Composition A 4.0 Betaine 1.5 Fragrance 0.5 Dye q.s.

[0480] Example Preparation 103: Bath Bomb

[0481] Ingredients ％ Sodium Bicarbonate 67.0 Citric Acid 9.0 Corn Starch 8.0 Butyrospermum Parkii Butter 5.0 Sodium Lauryl Sulfoacetate 4.0 Composition A 4.0 Theobroma Cacao Seed Butter 2.0 Theobroma Cacao (Cocoa) Fruit Powder 1.0 Fragrance, Dye q.s.

[0482] Example Preparation 104: Bath Salt

[0483] Ingredients ％ Maris Sal to 100 Fragrance 2.0 Glycolipid 1.0 Composition A 1.0 Silica 0.5 Simmondsia Chinensis Seed Oil 0.1 Preservative, Dye q.s.

[0484] Example Preparation 105: Bath Salt

[0485] Ingredients ％ Maris Sal to 100 Rosmarinus Officinalis Leaf Oil 3.0 Fragrance 1.5 Polysorbate 20 1.5 Composition A 1.5 Preservative, Dye q.s.

[0486] x-in-1 Example Preparation

[0487] Example Preparation 106: Body Wash and Shampoo

[0488]

[0489]

[0490] Example Preparation 107: Body Wash, Shampoo and Shaving Cream

[0491]

[0492]

[0493] Example Preparation 108: 2-in-1 Shower Gel and Shampoo

[0494] Ingredients ％ Water to 100 Sodium Laureth Sulfate 8.5 Glycerol 2.5 Sodium Cocoyl Isethionate 2.5 Ammonium Lauryl Sulfate 2.5 Lauryl Betaine 2.5 Lauryl Glucoside 1.5 Sodium Chloride 1.0 Composition A 1.0 Polyquaternium - 39 0.5 Polyquaternium - 7 0.5 Panthenol 0.1 Citric Acid at pH 5.5 Sodium Citrate 0.2 Propylene Glycol 0.3 PEG - 55 Propylene Glycol Oleate 0.7 Styrene / Acrylate Copolymer 0.2 Phenoxyethanol 0.7 Sodium Benzoate 0.3 Tetrasodium EDTA 0.1 Fragrance, Dye q.s.

[0495] Example Preparation 109: 2-in-1 Bubble Bath and Wash

[0496] Ingredients ％ Water to 100 Cocamidopropyl Betaine 4.0 Glycerol 4.0 Disodium Lauroamphodiacetate 2.0 Sodium Chloride 2.0 Cocoyl Glucoside 1.5 Hydroxypropyl Starch Phosphate 1.0 Disodium Lauroamphodiacetate 1.0 Composition A 1.0 Lauric Acid 1.0 Acrylate / Acrylic C10 - 30 Alkyl Ester Crosslinked Polymer 1.0 Polyquaternium - 7 1.0 Styrene / Acrylate Copolymer 0.5 Citric Acid at pH 5.5 Sodium Hydroxide 0.3 Sodium Benzoate 0.2 Benzoic Acid 0.1 Fragrance, Dye q.s.

[0497] Example Preparation 110: Body and Face Wash

[0498]

[0499]

[0500] Example Preparation 111: Hair and Body Shampoo

[0501]

[0502]

[0503] Example Preparation 112: After-Sun Hair and Body Shampoo

[0504]

[0505]

[0506] Example Preparation 113: Hair and Body Shampoo & Shower Gel

[0507] Ingredients ％ Water to 100 Sodium Laureth Sulfate 7.0 Sodium Chloride 3.8 Cocamidopropyl Betaine 3.5 Composition A 3.0 Panthenol 0.3 Hydrolyzed Keratin 0.7 Taurine 0.5 Simmondsia Chinensis Seed Oil 0.5 Disodium Lauroamphodiacetate 0.5 Citric Acid at pH 5.0 Sodium benzoate 0.7 Cocamide MEA 0.4 PEG-7 glyceryl cocoate 0.3 Polyquaternium-10 0.2 Propylene glycol 0.3 PEG-40 hydrogenated castor oil 0.4 Linalool 0.2 Geraniol 0.1 Butylphenyl methylpropional 0.1 Dye q.s.

[0508] Facial Cleansing Example Preparations

[0509] Example Preparation 114: Eye Makeup Remover

[0510]

[0511]

[0512] Example Preparation 115: Eye Makeup Remover

[0513] Ingredients ％ Water up to 100 Cyclopentasiloxane 8.0 Glycerin 6.0 Isocetane 6.0 Butylene glycol 4.0 Panthenol 3.0 Composition A 3.0 Sodium chloride 2.0 Disodium EDTA 1.0 Sodium hydroxide 0.2 Citric acid at pH 7 Phenoxyethanol 0.2 Methylparaben 0.1

[0514] Example Preparation 116: Micellar Lotion

[0515]

[0516]

[0517] Example Preparation 117: Waterproof Eye Makeup Remover

[0518] Ingredients ％ Water up to 100 Aloe Barbadensis leaf juice 5.0 Sorbitol 3.0 Glycerin 3.0 Panthenol 2.2 Sodium lauroyl glucamate 2.0 Biosaccharide gum-1 1.5 Lauryl glucoside 1.0 Composition A 0.5 Allantoin 0.2 Phenoxyethanol 0.2 Sodium benzoate 0.2 Sorbic acid q.s. Citric acid at pH 6

[0519] Example Preparation 118: Waterproof Eye Makeup Remover

[0520]

[0521]

[0522] Example Preparation 119: Micellar Gel

[0523] Ingredients ％ Water up to 100 Propylene glycol 5.0 Composition A 3.0 Glycerin, water, sodium acetylpropionate, sodium anisate 3.0 Polyglyceryl-4 caprate 2.0 Xanthan gum 1.5 Lactobacillus fermentation product 0.05 Lactic acid at pH 5.5

[0524] Example Preparation 120: Micellar Gel

[0525]

[0526]

[0527] Example Preparation 121: Micellar Makeup Remover

[0528] Ingredients ％ Water up to 100 Aloe Barbadensis leaf juice 4.0 Octyl / decyl glucoside 2.0 Glycolipid 1.5 Composition A 1.5 Betaine 1.0 Glycerin 1.0 Benzyl alcohol, caprylyl glycol, benzoic acid 1.0 Decyl glucoside 0.5 Fragrance q.s. Citric acid at pH 7.0

[0529] Example Preparation 122: Facial Cleansing Foam

[0530]

[0531]

[0532] Example Preparation 123: Facial Cleansing Foam

[0533]

[0534]

[0535] Example Preparation 124: Cleansing Foam

[0536]

[0537] Example Preparation 125: Cleansing Foam

[0538]

[0539]

[0540] Example Preparation 126: Cleansing Foam

[0541] Ingredients ％ Water up to 100 Glycerin 3.0 Decyl glucoside 3.0 Glycolipid 2.0 Pentylene glycol 2.0 Composition A 1.0 Citric acid at pH 6.0 Papain 0.1

[0542] Example Preparation 127: Pump Foam for Facial Cleansing

[0543]

[0544]

[0545] Example Preparation 128: Pump Foam for Facial Cleansing

[0546] Ingredients ％ Water up to 100 Sucrose 12.0 Glycerin 6.0 Rosa Damascena flower water 5.0 Cocoyl glucoside 4.5 Composition A 2.5 Water, sodium acetylpropionate, potassium sorbate 2.0 Sodium cocoyl glutamate 3.0 Polyglyceryl-4 caprate 3.0 Betaine 1.0 Citric acid at pH 5.0 Fragrance 0.8 Sodium chloride 0.5

[0547] Example Preparation 129: Micellar Water

[0548]

[0549]

[0550] Example Preparation 130: Refreshing Micellar Water

[0551] Ingredients ％ Water up to 100 Methylpropanediol 3.0 Propylene glycol 2.5 Glycerin 2.5 Sodium cocoyl amphoacetate 2.0 Glycolipid 1.5 Fragrance 0.8 Composition A 0.5 Sodium salicylate 0.5 Sodium benzoate 0.5 Citric acid at pH Sodium chloride 0.2 Tetrasodium EDTA 0.1

[0552] Example Preparation 131: Oil-Infused Micellar Water

[0553]

[0554]

[0555] Example Preparation 132: Facial Tonic

[0556]

[0557]

[0558] Example Preparation 133: Facial Tonic

[0559] Ingredients ％ Water up to 100 Glycerin 5.0 Octyl / decyl glucoside 2.0 Composition A 1.5 Glyceryl caprylate 1.5 Fragrance 1.0 Sodium acetylpropionate 1.0 Sodium anisate 1.0 Sodium salicylate 1.0 Sodium cocoyl glutamate 0.1 Polyglyceryl-6 oleate 0.1 Disodium cocoyl glutamate 0.1 Citric acid at pH 5.5 Sodium Surfactin 0.05

[0560] Example Preparation 134: Facial Tonic

[0561] Ingredients ％ Water up to 100 PEG-6 caprylic / capric glyceride 4.0 Composition A 3.0 Fructooligosaccharide 1.0 Mannitol 1.0 Xylitol 1.0 Propylene glycol 1.0 Cetrimonium bromide 1.0 Disodium EDTA 0.4 Rhamnose 0.1

[0562] Example Preparation 135: Micellar Water

[0563] Ingredients ％ Water up to 100 Glycerin 5.0 Polyglyceryl-4 caprate 5.0 Betaine 3.0 Composition A 2.0 Sodium acetylpropionate 2.0 Lactobacillus fermentation product 0.2 Sodium benzoate 0.2 Sodium hydroxide 0.2 Fragrance 0.1 Lactic acid at pH 5.8

[0564] Example formulation 136: Micellar water

[0565] Ingredients ％ Water up to 100 Glycerin 6.0 Poloxamer 184 2.5 Phenoxyethanol 1.0 PEG-40 hydrogenated castor oil 1.0 Lactobacillus extract, propylene glycol 0.8 Composition A 0.5 Fragrance 0.5 Citric acid at pH 6.4 Sodium hydroxide 0.2 Disodium EDTA 0.1

[0566] Example formulation 137: Micellar water

[0567]

[0568]

[0569] Example formulation 138: Micellar water

[0570]

[0571]

[0572] Example formulation 139: Micellar water

[0573] Ingredients ％ Water up to 100 Hexylene glycol 1.4 Glycerin 1.2 Composition A 1.0 Poloxamer 184 1.0 Disodium cocoamphodiacetate 1.0 Disodium EDTA 0.5 Polyaminopropyl biguanide 0.2 Lactic acid at pH 5.4

[0574] Example formulation 140: Micellar water

[0575]

[0576]

[0577] Example formulation 141: Facial cleanser and scrub

[0578]

[0579]

[0580] Microemulsion example formulations

[0581] Example formulation 142: Microemulsion for makeup removal

[0582] Ingredients ％ Water up to 100 1,3-Butanediol 15.0 Polyglyceryl-4 caprate 6.0 Polyglyceryl-3 caprate 6.0 Composition A 6.0 Glycerin 6.0 Isocetane 3.0 Sodium benzoate 0.8 Citric acid at pH 5.0

[0583] Example formulation 143: Microemulsion for makeup removal

[0584] Ingredients ％ Water up to 100 1,3-Butanediol 7.5 Polyglyceryl-4 Decanoate 5.5 Glycolipid 3.0 Composition A 1.5 Glycerin 5.0 Sodium Benzoate 0.8 Citric Acid Ad pH 5.0

[0585] Toothpaste example formulations

[0586] Example formulation 144: Toothpaste

[0587] Ingredients ％ Water up to 100 Glycerin 29.0 Sodium Metaphosphate 12.0 Hydrated Silica (Hydrated) 10.0 Xylitol 10.0 Composition A 1.0 Olaflur 0.13 Cocamidopropyl Betaine 0.5 Propylene Glycol 1.0 Chamomilla Recutita Flower Extract 0.4 Denatured Ethanol 0.5 Panthenol 0.1 Sodium Fluoride 0.1 Hydroxyethylcellulose 1.0 Titanium Dioxide 0.4 Fragrance 1.0 Citronellol 0.0 Eucalyptol 0.2 Eugenol 0.1 Menthol 0.15 Dyes, pH Regulators, Preservatives q.s.

[0588] Example Preparation 145: Toothpaste

[0589]

[0590]

[0591] Example Preparation 146: Toothpaste

[0592] Ingredients ％ Sorbitol 45.0 Water up to 100 Hydrated Silica (Hydrated) 18.0 Sodium Lauryl Sulfate 1.05 Composition A 0.75 PEG-32 1.0 Flavor 1.0 Cellulose Gum 0.8 Cocamidopropyl Betaine 0.35 Sodium Saccharin 0.2 Sodium Fluoride 0.23 Zinc Sulfate 0.3 Mica 0.5 Dye Solution (1% FD&C Blue No.1) 0.02 Titanium Dioxide 0.3 Eugenol 0.2 pH Regulators, Preservatives q.s.

[0593] Example Preparation 147: Toothpaste

[0594]

[0595]

[0596] Example Preparation 148: Toothpaste

[0597]

[0598]

[0599] Example Preparation 149: Toothpaste

[0600] Ingredients ％ Glycerin 27.0 Hydrated Silica (Hydrated) 21.0 Sodium Hexametaphosphate 13.0 Water up to 100 PEG-6 5.0 Flavor 1.0 Trisodium Phosphate 1.0 Sodium Lauryl Sulfate 1.05 Composition A 0.35 Chondrus Crispus 0.7 Cocamidopropyl Betaine 0.1 Sodium Saccharin 0.2 Polyethylene Glycol 0.5 Xanthan Gum 0.7 Sucralose 0.5 Mica 0.3 Titanium Dioxide 0.5 Sodium Fluoride 0.32 Dyes, pH Regulators, Preservatives q.s.

[0601] Example Preparation 150: Toothpaste

[0602]

[0603]

[0604] Example Preparation 151: Toothpaste

[0605]

[0606]

[0607] Example Preparation 152: Toothpaste

[0608] Ingredients ％ Water up to 100 Hydrated Silica (Hydrated) 21.0 Xylitol 10.0 Sorbitol 10.0 Composition A 1.5 Betaine 0.5 Aloe Barbadensis Leaf Juice Powder 0.3 Xanthan Gum 0.5 Zinc Gluconate 0.5 Sodium Cocoyl Sulfate 0.5 Mentha Spicata Crispa Herb Oil 0.1 Calendula Officinalis Flower Extract 0.2 Chamomilla Recutita Flower Extract 0.2 L-Limonene 0.1 Mentha Arvensis Leaf Oil 0.1 Sodium Fluoride 0.32 Titanium Dioxide 0.4 PH Regulators, Preservatives q.s.

[0609] Example Preparation 153: Toothpaste

[0610]

[0611]

[0612] Example Preparation 154: Toothpaste

[0613]

[0614]

[0615] Example Preparation 155: Toothpaste

[0616] Ingredients ％ Calcium Hydrogen Phosphate Dihydrate 25.0 Water up to 100 Sorbitol 20.0 Glycerin 20.0 Methyl Salicylate 1.0 Sodium Lauryl Sulfate 0.9 Composition A 0.9 Titanium Dioxide 0.5 Chondrus Crispus 0.8 Cellulose Gum 0.5 Sodium Silicate 0.3 Hydrated Silica (Hydrated) 1.0 Thymol 0.1 Sodium Saccharin 0.2 Sodium Hydroxide 0.5 Menthol 0.3 Dye Solution (1% FD&C Blue No.1) 0.02 pH Regulators, Preservatives q.s.

[0617] Example Preparation 156: Toothpaste

[0618]

[0619]

[0620] Example Preparation 157: Toothpaste

[0621]

[0622]

[0623] Example Preparation 158: Toothpaste

[0624] Ingredients ％ Glycerin 33.0 PEG-8 5.0 Hydrated Silica (Hydrated), 5.0 Calcium Sodium Phosphosilicate 3.0 Cocamidopropyl Betaine 1.0 Composition A 1.0 Sodium Methyl Cocoyl Taurate 0.3 Fragrance 0.8 Titanium Dioxide 0.5 Carbomer 0.5 Sodium Saccharin 0.2 Sodium Fluoride 0.32 Limonene 0.2 Dyes, pH Regulators, Preservatives q.s.

[0625] Example Preparation 159: Toothpaste

[0626]

[0627]

[0628] Example Preparation 160: Toothpaste

[0629]

[0630]

[0631] Example Preparation 161: Toothpaste

[0632]

[0633]

[0634] Example Preparation 162: Toothpaste

[0635] Ingredients ％ Water up to 100 Sorbitol 50.0 Hydrated Silica (Hydrated), 14.0 Silica Dimethyl Silylate 3.5 Hydroxyethylcellulose 3.5 Composition A 1.05 PEG-40 Hydrogenated Castor Oil (Hydrogenated) 0.8 Fragrance 0.9 Sodium Gluconate 0.4 Limonene 0.1 PEG-3 Tallowaminopropylamine 0.5 Olaflur 0.06 Stannous Fluoride 0.4 Sodium Saccharin 0.2 Potassium Hydroxide 0.4 Hydrochloric Acid 0.2 Dye Solution (1% FD&C Blue No.1) 0.04 pH regulator, preservative sufficient quantity to make

[0636] Example Preparation 163: Toothpaste

[0637]

[0638]

[0639] Example Preparation 164: Toothpaste

[0640]

[0641]

[0642] Example Preparation 165: Toothpaste

[0643] Ingredient ％ Glycerin 33.0 Hydrated silica 22.0 Water up to 100 Sorbitol 15.0 Potassium pyrophosphate 3.5 Composition A 1.5 Titanium dioxide 0.4 Sodium lauroyl sarcosinate 0.5 Cellulose gum 0.8 Lauryl glucoside 0.5 PVP 0.5 Cocamidopropyl betaine 0.5 Xanthan gum 0.5 Stannous fluoride 0.4 Dye, pH regulator, preservative sufficient quantity to make

[0644] Example Preparation 166: Toothpaste

[0645]

[0646]

[0647] Example Preparation 167: Toothpaste

[0648] Ingredient ％ Water up to 100 Hydrogenated starch hydrolysate (hydrogenated, hydrolyzed) 25.0 Hydrated silica (hydrated) 21.0 Hydroxyapatite 4.5 PEG - 32 4.5 Composition A 1.0 Sodium dodecyl sulfate 0.5 Fragrance 0.9 Sodium monofluorophosphate 0.76 Trisodium phosphate 0.5 Cellulose gum 1.0 Sodium saccharin 0.2 Sodium hydroxide 0.3 Limonene 0.1 Dye solution (1% FD&C Blue No.1) 0.05 Titanium dioxide 0.4 pH regulator, preservative sufficient quantity to make

[0649] Example Preparation 168: Toothpaste

[0650] Ingredient ％ Glycerin 32.0 Hydrated silica (hydrated) 21.0 Water up to 100 Sodium bicarbonate 30.0 PEG - 12 3.0 Composition A 1.5 Sodium dodecyl sulfate 1.0 Flavor 1.0 Sodium hydroxide 1.0 Cellulose gum 1.0 Chondrus Crispus 0.7 Sodium saccharin 0.15 Calcium peroxide 0.5 Titanium dioxide 0.5 Sodium monofluorophosphate 0.76 Dye, pH regulator, preservative sufficient quantity to make

[0651] Example Preparation 169: Toothpaste

[0652]

[0653]

[0654] Example Preparation 170: Toothpaste

[0655] Ingredient ％ Water up to 100 Calcium carbonate 30.0 Silica 7.0 Glycerin 5.0 Hydrated silica 2.5 Composition A 1.5 Fragrance 1.0 Chondrus Crispus powder 0.5 Cellulose gum 0.5 Chlorhexidine gluconate 0.1 Limonene 0.15 Sodium saccharin 0.15 Titanium dioxide 0.1 Sodium fluoride 0.32 Dye, pH regulator, preservative sufficient quantity to make

[0656] Example Preparation 171: Toothpaste

[0657]

[0658]

[0659] Example Preparation 172: Toothpaste

[0660]

[0661]

[0662] Example Preparation 173: Toothpaste

[0663] Ingredient ％ Water up to 100 Hydrated silica 15.0 Glycerin 15.0 Potassium nitrate 5.0 Sorbitol 4.5 Sodium cocoamphoacetate 0.5 Composition A 0.5 PEG - 40 hydrogenated castor oil 0.5 Carboxymethyl cellulose 0.5 Titanium dioxide 0.1 Sodium fluoride 0.32 Sodium saccharin 0.2 Fragrance 0.2 Chlorhexidine digluconate 0.12 Dye, pH regulator, preservative sufficient quantity to make

[0664] Example Preparation 174: Children's Toothpaste

[0665]

[0666]

[0667] Example Preparation 175: Children's Toothpaste

[0668] Ingredient ％ Sorbitol 33.0 Water up to 100 Hydrated silica (hydrated) 22.0 Composition A 1.0 Sodium C14 - 16 olefin sulfonate 1.0 Fragrance 0.8 Cellulose gum 0.8 Calcium glycerophosphate 0.5 Olaflur 0.07 Sodium fluoride 0.1 Propylene glycol 0.5 Sodium saccharin 0.2 Cocamidopropyl betaine 0.35 Mica 0.5 Citral 0.1 Limonene 0.1 Linalool 0.1 Titanium dioxide 0.4 Dye solution (1% FD&C Blue No.1) 0.05 Olaflur 0.07 pH regulator, preservative sufficient quantity to make

[0669] Example Preparation 176: Infant Toothpaste

[0670] Ingredient ％ Water up to 100 Hydrogenated starch hydrolysate (hydrogenated, hydrolyzed) 14.0 Hydrated silica (hydrated) 14.0 PEG - 32 3.5 Silica 7.0 Composition A 1.5 Cellulose gum 1.0 Fragrance 1.0 Propylene glycol 0.7 PEG - 40 hydrogenated castor oil (hydrogenated) 0.8 Olaflur 0.06 Sodium chloride 0.2 Sodium saccharin 0.15 Tocopheryl acetate 0.1 Glycerin 1.0 Retinyl palmitate 0.1 Dye solution (1% FD&C Blue No.1) 0.02 pH regulator, preservative sufficient quantity to make

[0671] Example Preparation 177: Children's Toothpaste

[0672]

[0673]

[0674] Example Preparation 178: Toddler Toothpaste

[0675] Ingredient ％ Water up to 100 Sorbitol 40.0 Hydrated silica (hydrated) 17.0 Glycerin 26.0 Composition A 1.5 Xanthan gum 1.0 Steareth - 30 1.0 Chondrus Crispus 1.0 Fragrance 1.0 Disodium hydrogen phosphate 0.5 Sodium benzoate 0.2 Amylase 0.1 Citric acid 0.3 Zinc gluconate 0.5 Glucose oxidase 0.1 Sodium fluoride 0.22 Sodium saccharin 0.15 Potassium thiocyanate 0.1 Lactoperoxidase 0.1 Titanium dioxide 0.3 Dye, pH regulator, preservative sufficient quantity to make

[0676] Example Preparation 179: 2-in-1 Toothpaste + Mouthwash

[0677] Ingredient ％ Sorbitol 38.0 Water up to 100 Hydrated silica (hydrated) 21.0 Pentasodium triphosphate 5.0 Sodium dodecyl sulfate 0.35 Composition A 1.05 PEG - 32 1.0 Fragrance 1.0 Aluminum oxide 0.5 Alcohol 0.7 Xanthan gum 0.8 Sodium fluoride 0.23 Sodium saccharin 0.2 Disodium hydrogen phosphate 0.5 Cocamidopropyl betaine 0.35 Zinc sulfate 0.5 Trisodium phosphate 0.3 Sodium chloride 0.4 Sodium sulfate 0.2 Limonene 0.1 Titanium dioxide 0.5 Dye, pH regulator, preservative q.s.

[0678] Example Preparation 180: 2-in-1 Toothpaste + Mouthwash

[0679]

[0680]

[0681] Mouthwash Example Preparation

[0682] Example Preparation 181: Alcohol-Free Mouthwash

[0683]

[0684]

[0685] Example Preparation 182: Alcohol-Free Mouthwash

[0686] Ingredients ％ Water ad 100 Sorbitol 15.0 Glycerin 7.5 Composition A 1.0 Xylitol 1.0 Cellulose Gum 0.5 PCA Zinc 0.5 Zinc Hydroxyapatite 0.05 Fragrance 0.9 Silica 0.05 Ricinus Communis Seed Oil 0.1 Ammonium Acryloyldimethyltaurate / VP Copolymer 0.05 Mentha Arvensis Leaf Oil 0.01 Sodium Myristoyl Sarcosinate 0.2 Sodium Methyl Cocoyl Taurate 0.2 Sodium Saccharin 0.5 Sodium Fluoride 0.05 Limonene 0.1 Dyes, pH Adjusters, Preservatives q.s.

[0687] Example Preparation 183: Alcohol-Free Mouthwash

[0688]

[0689]

[0690] Example Preparation 184: Alcohol-Free Mouthwash

[0691] Ingredients ％ Water ad 100 Glycerin 7.5 Composition A 0.5 Glycolipid 0.5 Flavor 0.3 Cetylpyridinium Chloride 0.04 Sodium Saccharin 0.05 Sucralose 0.05 Sodium Fluoride 0.02 Disodium Hydrogen Phosphate 0.25 Dyes, pH Adjusters, Preservatives q.s.

[0692] Example Preparation 185: Mouthwash

[0693]

[0694]

[0695] Example Preparation 43: Alcohol-free Mouthwash

[0696] Ingredients ％ Water ad 100 Sorbitol 15.0 Composition A 1.0 Aloe Barbadensis Leaf Extract 0.15 Calendula Officinalis Flower Extract 0.2 Chamomilla Recutita Flower Extract 0.2 Sodium Fluoride 0.02 Calcium Glycerophosphate 0.05 Zinc Gluconate 0.8 Mentha Spicata Crispa Herb Oil 0.05 Mentha Arvensis Leaf Oil 0.05 Menthol 0.04 Dyes, pH Adjusters, Preservatives q.s.

[0697] Example Preparation 186: Alcohol-free Mouthwash

[0698] Ingredients ％ Water ad 100 Glycerin 15.0 Sodium Gluconate 1.5 Composition A 0.5 PEG - 40 Hydrogenated Castor Oil 0.5 Olaflur 0.16 Fragrance 0.25 Stannous Chloride 0.05 Sodium Fluoride 0.02 Cocamidopropyl Betaine 0.35 Sodium Saccharin 0.07 Dyes, pH Adjusters, Preservatives q.s.

[0699] Example Preparation 187: Alcohol-free Mouthwash

[0700] Ingredients ％ Water ad 100 Glycerin 10.0 Sorbitol 8.0 Composition A 1.0 Glycolipid 0.5 Sodium Pyrophosphate 0.25 Fragrance 0.04 Limonene 0.1 Sodium Fluoride 0.02 Sodium Saccharin 0.08 Dyes, pH Adjusters, Preservatives q.s.

[0701] Example Preparation 188: Alcohol-free Mouthwash

[0702] Ingredients ％ Water ad 100 Glycerin 7.5 PEG - 60 Hydrogenated Castor Oil 1.0 Composition A 0.5 Sodium Citrate 0.45 Fragrance 0.3 Zinc Chloride 0.15 Cetylpyridinium Chloride 0.05 Sodium Saccharin 0.08 Sodium Fluoride 0.02 Dyes, pH Adjusters, Preservatives q.s.

[0703] Example Preparation 189: Alcohol-free Mouthwash

[0704] Ingredients ％ Water ad 100 Glycerin 11.0 Composition A 2.0 Flavor 1.0 Cetylpyridinium Chloride 0.5 Poloxamer 407 0.1 Sodium Saccharin 0.1 Sodium Fluoride 0.02 Sucralose 0.05 Mentha Viridis Leaf Oil 0.1 Dyes, pH Adjusters, Preservatives q.s.

[0705] Example Preparation 190: Alcohol-free Mouthwash

[0706]

[0707]

[0708] Example Preparation 191: Alcohol-free Mouthwash

[0709] Ingredients ％ Water ad 100 Sorbitol 10.0 PEG - 40 Hydrogenated Castor Oil 1.0 Potassium Citrate 1.0 Composition A 0.25 Glycerin 0.9 Fragrance 0.4 Zinc Sulfate 0.15 Sodium Saccharin 0.1 Propylene Glycol 0.3 Citrus Limon Juice 0.05 Aloe Barbadensis Leaf Extract 0.1 Limonene 0.05 Sodium Fluoride 0.02 Dyes, pH Adjusters, Preservatives q.s.

[0710] Example Preparation 192: Alcohol-free Mouthwash

[0711]

[0712]

[0713] Example Preparation 193: Mouthwash

[0714] Ingredients ％ Water ad 100 Glycerin 11.0 Propylene Glycol 7.5 Sorbitol 7.5 Potassium Pyrophosphate 1.5 Polysorbate 20 0.5 Composition A 0.5 Sodium Pyrophosphate 0.75 Zinc Citrate 0.15 PVM / MA Copolymer 0.1 Flavor 0.2 Sodium Fluoride 0.05 Sodium Saccharin 0.07 Dyes, pH Adjusters, Preservatives q.s.

[0715] Example Preparation 194: Mouthwash

[0716] Ingredients ％ Water ad 100 Glycerin 11.0 Alcohol 7.5 Sorbitol 7.5 Composition A 1.0 Poloxamer 407 0.5 Sodium Phosphate, Disodium Hydrogen Phosphate 0.15 Flavor 0.9 Cetylpyridinium Chloride 0.05 Sodium Saccharin 0.08 Sodium Fluoride 0.05 Dyes, pH Adjusters, Preservatives q.s.

[0717] Example Preparation 195: Mouthwash

[0718] Ingredients ％ Water ad 100 Alcohol 22.0 Sorbitol 10.0 Flavor 1.0 Composition A 1.0 Sodium Saccharin 0.09 Eucalyptol 0.1 Thymol 0.06 Sucralose 0.06 Sodium Fluoride 0.05 Menthol 0.04 Dyes, pH Adjusters, Preservatives q.s.

[0719] Example Preparation 196: Mouthwash

[0720] Ingredients ％ Water ad 100 Glycerin 12.0 Xylitol 7.0 Disodium hydrogen phosphate 1.5 Composition A 1.0 Cocamidopropyl betaine 0.5 Propylene glycol 0.5 Stannous fluoride 0.09 Fragrance 0.3 Peppermint (Mentha piperita) leaf extract 0.1 Chamomile (Chamomilla Recutita) flower extract 0.2 Sage (Salvia Officinalis) leaf extract 0.25 Myrrh resin (Commiphora Myrrha Resin) extract 0.15 Sodium saccharin 0.1 Citral 0.05 Limonene 0.02 Linalool 0.02 Dye, pH regulator, preservative q.s.

[0721] Example Preparation 55: Mouthwash

[0722]

[0723]

[0724] Example Preparation 197: Mouthwash

[0725] Ingredients ％ Water ad 100 Denatured ethanol 33.0 Glycerin 10.0 Sorbitol 8.0 PEG-40 hydrogenated castor oil 1.0 Composition A 1.0 Glycolipid 0.5 Fragrance 0.6 Menthol 0.05 Sodium saccharin 0.07 Aluminum lactate 0.2 Thymol 0.06 Turmeric (Curcuma Xanthorrhiza) root extract 0.05 Sodium fluoride 0.02 Dye, pH regulator, preservative q.s.

[0726] Example Preparation 198: Mouthwash

[0727]

[0728]

[0729] Example Preparation 199: Mouthwash

[0730] Ingredients ％ Water ad 100 Composition A 1.0 Eucalyptol 0.9 Zinc chloride 0.25 Methyl salicylate 0.05 Thymol 0.05 Levomenthol 0.07 Stannous fluoride 0.09 Dye, pH regulator, preservative q.s.

[0731] Example Preparation 200: Mouthwash

[0732]

[0733]

[0734] Example Preparation 201: Mouthwash

[0735]

[0736]

[0737] Example Preparation 202: Mouthwash

[0738] Ingredients ％ Water ad 100 Alcohol 15.0 Sorbitol 7.0 Sodium lauroyl sarcosinate 1.0 Composition A 0.25 PEG-40 hydrogenated castor oil 0.25 Fragrance 0.4 Cetylpyridinium chloride 0.04 Sodium citrate 0.15 Sodium saccharin 0.08 Sodium fluoride 0.05 Limonene 0.05 Dye, pH regulator, preservative q.s.

[0739] Example Preparation 203: Mouthwash

[0740]

[0741]

[0742] Example Preparation 204: Mouthwash

[0743] Ingredients ％ Water ad 100 Glycerin 10.0 Hydrogenated starch hydrolysate 1.0 PVP 0.5 Composition A 0.5 Fragrance 0.4 Zinc acetate 0.2 Sodium fluoride 0.32 Acesulfame potassium 0.15 Chlorhexidine digluconate 0.2 Dye, pH regulator, preservative q.s.

[0744] Example Preparation 205: Mouthwash

[0745]

[0746]

[0747] Example Preparation 206: Mouthwash

[0748] Ingredients ％ Water ad 100 Glycerin 15.0 Macrogolglycerol Hydroxystearate 7.0 Sorbitol 7.0 Composition A 0.5 Cocamidopropyl betaine 0.2 Sodium fluoride 0.02 Chlorhexidine digluconate 0.2 Flavor 0.5 Dye, pH regulator, preservative q.s.

[0749] Example Preparation 207: Mouthwash Concentrate

[0750] Ingredients ％ Propylene glycol 55.0 Water ad 100 Fragrance 2.0 Sodium saccharin 0.4 Composition A 0.75 Polysorbate 20 0.25 Myrrh (Commiphora Myrrha) oil 2.0 Sage (Salvia Officinalis) oil 0.2 Peppermint (Mentha piperita) oil 0.2 Chamomile (Chamomilla Recutita) flower extract 0.2 Limonene 0.1 Sodium fluoride 0.02 Dye, pH regulator, preservative q.s.

[0751] Example Preparation 208: Mouthwash Concentrate

[0752] Ingredients ％ Propylene glycol 45.0 PEG-6 5.0 Water ad 100 Composition A 1.0 Bisabolol 0.2 Zinc chloride 0.25 Cetylpyridinium chloride 0.1 Sodium saccharin 0.5 Sodium fluoride 0.05 Sage (Salvia Officinalis) oil 2.5 Eugenol 0.2 Limonene 0.1 Linalool 0.1 Citronellol 0.1 Dye, pH regulator, preservative q.s.

[0753] Example Preparation 209: Mouthwash Concentrate

[0754]

[0755]

[0756] Example Preparation 210: Peroxide Mouthwash

[0757] Ingredients ％ Water ad 100 Glycerin 11.0 Alcohol 5.0 <![CDATA[H 2 O 2 35%]]> 4.30 Polyphosphate 1.0 Menthol 0.05 Cooling agent 0.02 Flavor 0.15 Calcium chloride 0.03 Composition A 0.50 Sodium saccharin 0.1 Sodium fluoride 0.22 Dye, pH regulator, preservative q.s.

[0758] Example Preparation 211: Fluoride Gel

[0759]

[0760]

[0761] Example Preparation 212: Fluoride Gel

[0762] Ingredients ％ Water ad 100 Sorbitol 15.00 Silica 5.0 PEG 600 3.5 Potassium pyrophosphate 1.5 Xanthan gum 0.5 Sodium dodecyl sulfate 0.75 Composition A 0.75 Fragrance 0.9 Methol 0.05 Anethole 0.05 Citronellol 0.2 Sodium fluoride 5.0 Dye, pH regulator, preservative q.s.

[0763] Example Preparation 213: Dental Floss

[0764] Ingredients ％ PTFE ad 100 Fragrance 1.0 Acacia Senegal Gum 0.7 White beeswax (Cera Alba) 0.7 Potassium Acesulfame 0.5 Sodium Lauryl Sulfate 0.25 Composition A 0.25 Glycerol 0.7 Silicon Dioxide 0.5 Sodium Fluoride 0.22

[0765] Example Preparation 214: Teeth Wipe

[0766] Ingredient ％ Water q.s.100 Denatured Ethanol 30.00 Glycerol 20.00 Stevia 1.0 Polysorbate 20 0.5 Composition A 0.5 Fragrance 1.0 Dye, pH Regulator, Preservative q.s.

[0767] Example Preparation 215: Chewing Gum

[0768] Ingredient ％ Chewing Gum Base ad 100 Sorbitol 40.0 Isomalt 9.0 Xylitol 2.5 Mannitol D 2.5 Aspartame 0.1 Acesulfam K 0.05 Emulgum 0.2 Sorbitol 12.00 Glycerol 1.0 Composition A 1.0 Flavor 1.0 Sodium Fluoride 0.05 Dye, pH Regulator, Preservative q.s.

[0769] Example formulation 216: Mouthwash tablets

[0770]

[0771]

[0772] Example formulation 217: Tooth Tabs

[0773] Ingredient ％ Anhydrous Calcium Hydrogen Phosphate ad 100 Potassium Hydrogen Tartrate 15.0 Sorbitol 15.0 Composition A 6.0 Lauryl Sarcosine 0.8 Kaolin 0.5 Sodium Saccharin 0.5 Fragrance 0.2 Dye, pH Regulator, Preservative q.s.

[0774] Example formulation 218: Toothpaste powder

[0775]

[0776]

[0777] Additional example formulations (in wt%):

[0778]

[0779]

[0780]

[0781]

[0782]

[0783]

[0784]

[0785]

Claims

1. A composition comprising a) at least one sophorolipid b) lactic acid and / or its salts, characterized in that the content of a) is from 1.2% to 70.0% by weight and the content of b) is from 0.08% to 10.0% by weight, where the weight percentages are based on the total composition.

2. The composition according to claim 1, characterized in that the total content of monohydric alcohols selected from methanol, ethanol, propanol, isopropanol and butanol is from 0.0% to 10.0% by weight, preferably from 0.0% to 2.0% by weight, where the weight percentages are based on the total composition.

3. The composition according to claim 1 or 2, characterized in that it contains water, preferably in an amount of from 25.0% to 95.0% by weight, where the weight percentages are based on the total composition.

4. The composition according to any one of the preceding claims, characterized in that the weight ratio of a) to b) is from 5:1 to 30:

1.

5. The composition according to any one of the preceding claims, characterized in that component a) has a lactone-type sophorolipid content of more than 50% by weight, preferably more than 80% by weight, more preferably more than 95% by weight, where the weight percentages are based on the total of component a).

6. The composition according to any one of claims 1 to 5, characterized in that component a) has an acid-type sophorolipid content of from 55% to 99% by weight, preferably from 58% to 98% by weight, more preferably from 82% to 95% by weight, where the weight percentages are based on the total of component a).

7. The composition according to any one of the preceding claims, characterized in that in component b), the L-enantiomer content is higher than 51.0% by weight, preferably higher than 80.0% by weight, more preferably higher than 95% by weight, where the weight percentages are based on the total of component b).

8. The composition according to any one of the preceding claims, characterized in that it has a pH of from 3.5 to 11.0, preferably from 4.0 to 9.0, particularly preferably from 4.5 to 7.

0.

9. The composition according to any one of the preceding claims, characterized in that the content of a) is from 30.0% to 55.0% by weight, the content of b) is from 1.0% to 5.0% by weight, and the content of water is from 30.0% to 69.0% by weight, where the weight percentages are based on the sum of a), b) and water.

10. The composition according to any one of the preceding claims, characterized in that the content of a) is from 30.0% to 55.0% by weight, the content of b) is from 1.0% to 5.0% by weight, and the content of water is from 40.0% to 69.0% by weight, where the weight percentages are based on the total composition.

11. The composition according to any one of claims 1 to 8, characterized in that the content of a) is from 1.2% to 10.0% by weight, and the content of b) is from 0.08% to 3.0% by weight, where the weight percentages are based on the total composition.

12. The composition according to any one of the preceding claims, characterized in that It contains further biosurfactants, which are preferably selected from rhamnolipids, glycolipids, cellulose lipids, mannitol erythritol lipids and trehalose lipids, preferably rhamnolipids and glycolipids, and most preferably rhamnolipids.

13. Use of the composition according to any one of the preceding claims for cleaning a surface, which surface is preferably selected from the group consisting of the skin, the surface of tableware or the surface of textiles.

14. Use of the composition according to any one of claims 1 to 12 for moisturizing human skin.

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