Cattle and sheep parasite expelling auxiliary additive composition and preparation method thereof

Abstract

The invention discloses an insect-repelling auxiliary additive composition for cattle and sheep and a preparation method thereof, and particularly relates to a composite auxiliary additive composition for enhancing the effect of an insect-repelling drug and reducing the side effect of the drug and a preparation method of the composite auxiliary additive composition. The feed additive consists of the following components in percentage by mass: 5-8% of vitamin E, 10-15% of vitamin C, 0.05-0.1% of nano-selenium, 15-20% of saccharomyces cerevisiae (the viable count is greater than or equal to 1 * 10 < 9 > CFU / g), 10-15% of lactic acid bacteria (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), 3-5% of glutathione, 8-12% of choline chloride, 8-10% of mannan oligosaccharide, 2-3% of gamma-aminobutyric acid and the balance of montmorillonite. According to the invention, a four-dimensional synergistic system of oxidation resistance, immunoregulation, intestinal protection and nerve regulation is established for the first time, and through the interfacial effect of nano-selenium and organic components, the metabolic interaction of microorganisms and oligosaccharides and the space-time synergy of physical adsorption and biochemical detoxification, the insect expelling effect and animal health are both improved. The effect of the insect repellent can be enhanced, and the side effects caused by the insect repellent can be reduced.

Description

Technical Field

[0001] The invention relates to the technical field of livestock breeding additives, specifically to an auxiliary additive composition for deworming cattle and sheep and a preparation method thereof, in particular to a composite auxiliary additive composition for enhancing the effect of deworming drugs and reducing drug side effects and a preparation method thereof. Background Art

[0002] With the development of animal husbandry, the scale of cattle and sheep breeding is also expanding, and the number of cattle and sheep is increasing. Parasitic diseases in cattle and sheep, especially grazing and semi-grazing cattle and sheep, are frequent and serious, which not only affects the health of animals, but also becomes the main factor restricting the development of cattle and sheep breeding and the increase of farmers' and herdsmen's income. At present, the treatment of cattle and sheep parasites is to use drugs to expel or kill insects. Conventional anthelmintics often cause adverse reactions such as oxidative stress, intestinal flora imbalance, and liver damage in animals while killing parasites. The single use of antioxidants or probiotics in the prior art cannot systematically solve the above problems. The present invention innovatively achieves the dual goals of anthelmintic enhancement and toxicity reduction through the synergistic effect of multiple components. Summary of the invention

[0003] The object of the present invention is to provide an auxiliary additive composition for deworming cattle and sheep and a preparation method thereof, so as to solve the problems raised in the above-mentioned background technology.

[0004] To achieve the above object, the present invention provides the following technical solution: an auxiliary additive composition for deworming cattle and sheep, comprising the following components by mass percentage: 5-8% vitamin E, 10-15% vitamin C, 0.05-0.1% nano-selenium, and Saccharomyces cerevisiae (viable bacteria count ≥ 1×10 9 CFU / g) 15-20%, lactic acid bacteria (viable count ≥ 5×10 9 CFU / g) 10-15%, glutathione 3-5%, choline chloride 8-12%, manno-oligosaccharide 8-10%, γ-aminobutyric acid 2-3%, and montmorillonite as the balance.

[0005] A method for preparing a cattle and sheep deworming auxiliary additive composition specifically comprises the following steps: The first step is to ultrafinely grind an appropriate amount of montmorillonite to 200 meshes for later use; The second step is to weigh vitamin E, vitamin C, glutathione, choline chloride, and gamma-aminobutyric acid according to weight proportion, and then respectively perform rumen coating treatment for standby use; The third step is to add the ultrafine powder montmorillonite, nano selenium and the product of the second step into a V-type mixer and mix them thoroughly for at least 15 minutes; Step 4: Finally, add the corresponding weight ratios of Saccharomyces cerevisiae, Lactobacillus, and mannan oligosaccharide to the V-type mixer and mix for at least 20 minutes in an environment with a humidity < 30% to obtain the finished product; Step 5: Package the product according to the specifications and then store it in the dark.

[0006] Mechanism of action of each component: Vitamin E and Vitamin C: They form an antioxidant synergistic system to scavenge free radicals generated by the metabolism of anthelmintic drugs and maintain the integrity of cell membranes. VE is lipophilic and protects cell membranes, while VC is water-soluble and scavenges intracellular free radicals.

[0007] Nano-selenium: By activating the glutathione peroxidase system, it enhances the liver's detoxification function. Its nano-structure improves bioavailability and forms a ternary antioxidant network with VE and VC.

[0008] Saccharomyces cerevisiae + Lactobacillus: Build an intestinal microecological barrier and competitively inhibit the attachment of parasites. The mannan produced by the metabolism of yeast and mannan oligosaccharide in the formula synergistically activate intestinal TLR receptors and enhance mucosal immunity.

[0009] Glutathione: Directly neutralizes the toxicity of the metabolic intermediates of anthelmintic drugs and synergistically enhances the activity of hepatocyte detoxifying enzymes with choline chloride (increasing the efficiency of the CYP450 enzyme system by 30 - 45%).

[0010] Mannan oligosaccharide: Selectively promotes the proliferation of Bifidobacterium. Its β-1,3 glycosidic bond structure is similar to the surface antigen of parasites, inducing cross-immune reactions.

[0011] γ-Aminobutyric acid: Inhibits the overexcitation of the sympathetic nerve and reduces the increase in cortisol levels caused by anthelmintic stress by 38 - 52%.

[0012] Montmorillonite: Its layered structure adsorbs toxins released by parasites and excessive bile acids, and its cation exchange capacity regulates the intestinal electrolyte balance.

[0013] Compared with the prior art, the beneficial effects of the present invention are: In the present invention, antioxidant-detoxification synergy: VE + VC + nano-selenium + glutathione form a four-level antioxidant system, which acts on the cell membrane, cytoplasm, enzyme system, and small molecule detoxification levels respectively, and the total antioxidant capacity is increased by 2.3 - 2.8 times; immune regulation synergy: β-glucan and mannan oligosaccharide in the yeast cell wall synergistically activate macrophages and dendritic cells, increasing the IgA secretion by 65 - 80%; intestinal protection synergy: the physical adsorption of montmorillonite and the organic acids produced by the metabolism of Lactobacillus jointly maintain the intestinal pH, reducing the hatching rate of parasite eggs by 42 - 55%.

[0014] The present invention establishes for the first time a four-dimensional synergistic system of "antioxidation-immunomodulation-intestinal protection-neural regulation", which achieves both the improvement of anthelmintic effect and animal health through the interfacial effect of nano-selenium and organic components, the metabolic interaction of microorganisms and oligosaccharides, and the spatiotemporal synergy of physical adsorption and biodetoxification. It can enhance the effect of anthelmintic drugs and reduce the side effects of anthelmintic drugs. DETAILED DESCRIPTION

[0015] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0016] Example 1, a cattle and sheep deworming auxiliary additive composition, composed of the following components in percentage by weight: vitamin E 6%, vitamin C 10%, nano-selenium 0.1%, saccharomyces cerevisiae (viable bacteria count ≥ 1×10 9 CFU / g) 15%, lactic acid bacteria (viable count ≥ 5×10 9 CFU / g) 10-15%, glutathione 5%, choline chloride 10%, manno-oligosaccharide 10%, γ-aminobutyric acid 3%, and montmorillonite as the balance.

[0017] A method for preparing a cattle and sheep deworming auxiliary additive composition specifically comprises the following steps: The first step is to ultrafinely grind an appropriate amount of montmorillonite to 200 meshes for later use; The second step is to weigh vitamin E, vitamin C, glutathione, choline chloride, and gamma-aminobutyric acid according to weight proportion, and then respectively perform rumen coating treatment for standby use; The third step is to add the ultrafine powder montmorillonite, nano selenium and the product of the second step into a V-type mixer and mix them thoroughly for at least 15 minutes; Step 4: finally, add the corresponding weight proportions of saccharomyces cerevisiae, lactic acid bacteria and manno-oligosaccharide into a V-type mixer, and mix for at least 20 minutes in an environment with a humidity of <30% to obtain a finished product; The fifth step is to package the products according to the specifications and store them away from light.

[0018] Two groups of cattle were dewormed with albendazole for beef cattle. One group of cattle had 1% of this agent added to the deworming drug. One week later, the data of the two groups of cattle were compared. Compared with the blank experimental group, the negative conversion rate of worm eggs in the group of cattle added with this agent increased to 98.7% (89.2% for the control group), the serum MDA level decreased by 57.3%, the ALT activity decreased by 42.8%, and the duration of stress response was shortened by 62%.

[0019] Although embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cattle and sheep deworming auxiliary additive composition, characterized in that: It is composed of the following components by mass percentage: vitamin E 5-8%, vitamin C 10-15%, nano-selenium 0.05-0.1%, brewer's yeast (viable count ≥ 1×10 9 CFU / g) 15-20%, lactic acid bacteria (viable count ≥ 5×10 9 CFU / g) 10-15%, glutathione 3-5%, choline chloride 8-12%, manno-oligosaccharide 8-10%, γ-aminobutyric acid 2-3%, and montmorillonite as the balance.

2. A method for preparing a cattle and sheep anthelmintic additive composition, characterized in that: The specific steps include: The first step is to ultrafinely grind an appropriate amount of montmorillonite to 200 meshes for later use; The second step is to weigh vitamin E, vitamin C, glutathione, choline chloride, and gamma-aminobutyric acid according to weight proportion, and then respectively perform rumen coating treatment for standby use; The third step is to add the ultrafine powder montmorillonite, nano selenium and the product of the second step into a V-type mixer and mix them thoroughly for at least 15 minutes; Step 4: finally, add the corresponding weight proportions of saccharomyces cerevisiae, lactic acid bacteria and manno-oligosaccharide into a V-type mixer, and mix for at least 20 minutes in an environment with a humidity of <30% to obtain a finished product; The fifth step is to package the products according to the specifications and store them away from light.