Anti-reversal growth type stable fertilizer and preparation method thereof

Abstract

This invention discloses a stress-resistant and growth-promoting stable fertilizer and its preparation method, belonging to the field of bio-organic fertilizer technology. This invention uses fermented soybean meal, pomegranate peel residue, Eucommia ulmoides leaf residue, compound microbial inoculum, and microbial activators as raw materials to prepare bio-organic fertilizer. Specifically, this invention screens two highly salt-tolerant and growth-promoting microbial strains to form functional microorganisms. *A. chinensis* activates the enzyme production activity of salt-tolerant *Microbacillus maculatus* through secretion of metabolites. The organic matter decomposed by the salt-tolerant *Microbacillus maculatus*, in turn, provides growth substrates for *A. chinensis*, forming a metabolic mutualistic relationship. Using this fertilizer can effectively regulate and stabilize soil activity and promote crop growth. Under salt stress, it can effectively increase the activity of stress-related enzymes, root vitality, and carotenoid content in leaves, improving the resistance of soybean plants to salt stress adverse conditions, and has broad development potential and application value.

Description

Technical Field

[0001] This invention belongs to the field of bio-organic fertilizer technology, specifically relating to a stress-resistant and growth-promoting stable fertilizer and its preparation method. Background Technology

[0002] With the intensification of agricultural production and the overuse of chemical fertilizers, problems such as soil degradation, environmental pollution, and decreased crop resistance are becoming increasingly serious. Although traditional chemical fertilizers can increase crop yields in the short term, long-term use can lead to soil compaction, microbial community imbalance, and decreased organic matter content, thereby affecting soil health and sustainable agricultural development.

[0003] Bio-organic fertilizer is a type of fertilizer that combines organic matter with beneficial microorganisms. Through microbial activity, it improves the soil environment and promotes plant growth. Currently, bio-organic fertilizers are mostly made from agricultural waste such as livestock and poultry manure and straw, with the addition of functional microorganisms such as nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, and potassium-solubilizing bacteria. While these fertilizers can improve soil fertility to some extent, their effects on stress resistance, plant growth promotion, and soil stability enhancement are limited.

[0004] Stress resistance and growth promotion refer to enhancing plant tolerance to adverse conditions and promoting plant growth through biological or chemical means. Current technologies mainly achieve this by adding plant growth regulators (such as gibberellins and abscisic acid) or functional fertilizers. However, these methods suffer from high costs, unstable effects, and environmental unfriendliness. Soil stability refers to the soil's ability to resist erosion and maintain its structure and fertility. Current technologies mainly improve soil structure by adding organic matter (such as humic acid) or mineral materials (such as bentonite). However, these methods have limited effects on regulating soil microbial communities and soil enzyme activity, making it difficult to fundamentally improve soil biological activity and ecological functions.

[0005] For example, Chinese patent application CN202210045898.9 discloses a bio-organic fertilizer for promoting the growth and efficiency of degraded farmland and its manufacturing method, which is composed of the following raw materials: cottonseed meal, straw powder, potassium fulvic acid, urea ammonium nitrogen fertilizer, potassium humate, magnesium ammonium phosphate, ammonium polyphosphate, hydroxypropyl methylcellulose, tricalcium silicate, potassium nitrate phosphate fertilizer, soybean meal, nano-modified charcoal, glucose, amino acids, peptone, sophorolipid, Bacillus subtilis, Bacillus, actinomycetes, nitrogen-fixing bacteria, and sodium tripolyphosphate. This invention utilizes microbial fermentation combined with organic raw materials and inorganic substances to fully stimulate the interaction and growth-promoting mechanism of the plant-soil-microorganism system. By enhancing the dominant microbial community, it activates soil nutrients and increases soil volume, thereby improving crop resistance, promoting crop growth, repairing degraded farmland, and increasing the productivity of degraded farmland.

[0006] Currently, how to develop a highly efficient and simple bio-organic fertilizer that can effectively promote crop stress resistance and growth, regulate soil activity, and provide stability is a pressing technical problem that needs to be solved. Summary of the Invention

[0007] This invention addresses the problems existing in the prior art by screening two functional microbial strains. After being combined, they can fully ferment organic materials and have good growth-promoting effects and salt tolerance. When applied to the soil, they can effectively promote crop growth and stabilize and enhance soil activity while providing rich nutrients to crops.

[0008] To achieve the above-mentioned technical objectives, the technical solution adopted by the present invention is as follows:

[0009] A stress-resistant and growth-promoting stable fertilizer includes the following raw materials: fermented soybean meal, pomegranate peel residue, eucommia leaf residue, compound microbial inoculum, and microbial activator.

[0010] Furthermore, the composite microbial culture includes salt-tolerant and alkali-tolerant small bacilli (… 嗜碱嗜盐杆菌属 嗜盐碱杆菌 ) and Madura hygroscopica ( 奇贝岛马杜拉放线菌 The volume ratio of the two is 1:1.

[0011] Furthermore, the strain of the salt-tolerant *Microbacterium malathione* is CGMCC No. 1.4011, with an original deposit date of May 1, 2005; the strain of *Actinomyces madurae* is CGMCC No. 4.6236, with an original deposit date of September 3, 2008. Both strains were purchased from the conservation center and can be obtained through open commercial channels without the need for biological preservation.

[0012] Furthermore, the microbial activator includes trehalose, chitosan, fish protein powder, potassium humate, and bayberry leaf extract in a mass ratio of 1:1:15:3:2.

[0013] Furthermore, the preparation method of the bayberry leaf extract is as follows: collect bayberry branches and leaves, rinse with clean water to remove mud and sand, drain the water, use a pulverizer to pulverize the branches and leaves into 1-2cm fragments, spread them on a drying ground to air dry naturally for 3-5 days, the moisture content of the pulverized material is ≤15%; mix the pulverized material with water at a ratio of 1:10, add 1%-3% citric acid to the mixture, soak at a constant temperature of 60-80℃ for 10-15 hours, separate the solid and liquid to obtain the extract, and collect the bayberry leaf filter residue for later use; mix the extract with zeolite powder at a ratio of 1:3, stir to adsorb, spread out to air dry until the moisture content is ≤20%, add 3-5kg zinc sulfate per ton of adsorbate, mix evenly to obtain the bayberry leaf extract.

[0014] A method for preparing a stress-resistant and growth-promoting stable fertilizer includes the following preparation steps:

[0015] (1) Preparation of soybean meal fermentation product: Crush soybean meal to 60-80 mesh, add 5% of the soybean meal mass of a compound enzymatic hydrolysate containing glucose, enzymatically hydrolyze at 50-55℃ for 4 hours, then inoculate with lactic acid bacteria at 3% of the soybean meal mass, and anaerobic ferment at 35-37℃ for 72 hours to obtain soybean meal fermentation product.

[0016] (2) Treatment of pomegranate peel residue and eucommia leaf residue: After the pomegranate peel residue is exploded with steam at 1-1.2MPa for 5-10 minutes, it is mixed with eucommia leaf residue at a mass ratio of 2:1; then neutralized with lime water with a mass concentration of 1% to pH 6.5-7.0, dried and pulverized to 100 mesh to obtain a mixture of pomegranate peel residue and eucommia leaf residue.

[0017] (3) Preparation of compound microbial culture: After activating salt-tolerant Microbacterium maltophilum and Actinobacillus maculatus, they were inoculated into liquid culture medium and fermented at 28-36℃ for no less than 30 hours, with an effective viable count ≥10. 8 CFU / mL, then mix the two bacterial solutions at a volume ratio of 1:1, centrifuge to collect the bacterial cells, and prepare a compound microbial bacterial solution;

[0018] (4) Mix the filter residue of bayberry leaf extract, fermented soybean meal, pomegranate peel residue, and eucommia leaf residue in a mass ratio of 0.5:1:3 and crush them to a particle size of ≤5 mm to ensure uniform texture. Add rice husk powder or straw powder, adjust the carbon-nitrogen ratio of the mixture to 25-30:1, add water to adjust the moisture content to 60-65%, and obtain the mixed fermented material.

[0019] (5) Add compound microbial liquid at 1-4% of the total mass of the mixed fermentation material, spray it evenly into the mixed fermentation material, add microbial activator at 1% of the mass of the mixed fermentation material, mix thoroughly with a mixer, and carry out composting fermentation.

[0020] (6) Composting fermentation: In the early stage of composting, the temperature is raised to 55-65℃ and fermentation continues for 3-4 days; in the 4-10 days, the temperature is maintained at 50-60℃, and the pile is turned once a day to promote aerobic fermentation; in the 11-20 days, the temperature is naturally cooled down and fermented, and when the temperature drops below 40℃, the turning frequency is changed to once every 3 days to promote the formation of humus; the moisture content is maintained at 55-65% throughout the process, and water is added or the pile is turned to remove moisture when necessary; the material is dark brown and loose, odorless, and the pH is stable at 7.0-8.0. The seed germination index is ≥80% to complete the fermentation.

[0021] (7) Dry the decomposed material obtained in step (6) until the moisture content is ≤20%, crush it and sieve it, add 2-5% binder to granulate it, and control the particle size at 2-3 mm to obtain the finished fertilizer.

[0022] Furthermore, the preparation method of the glucose-containing complex enzymatic hydrolysate in step (1) is as follows: add 5g glucose, 1g cellulase, and 2g neutral protease to 3 liters of water, stir until dissolved, add 10mL of white vinegar, and the pH is approximately 5.5.

[0023] Furthermore, the liquid culture medium used in step (3) of the salt-tolerant microbacterium consists of: 10 g / L peptone, 5 g / L yeast extract, 1.5 g / L NaCl, and pH 7.0. Sterilization conditions: autoclaving at 121°C for 20 minutes.

[0024] Step (3) The liquid culture medium used for the *Mandula chinensis* consists of: peptone: 10 g / L, NaCl: 1.5 g / L, yeast extract: 5 g / L, glucose: 5 g / L, pH: 7.0-7.5 (adjusted with 1 mol / L NaOH or HCl), and sterilization conditions: autoclaving at 121℃ for 20 minutes.

[0025] Furthermore, in step (6), the composting adopts a windrow structure with an initial pile width of 1.5-2m and a pile height of 1.0-1.2m. Bamboo rafts or wooden frames are laid at the bottom to ensure ventilation and control the temperature.

[0026] The fertilizer of this invention is applied as a base fertilizer, at a rate of 200-300 kg / mu for conventional fields and 400-500 kg / mu for infertile fields. It is particularly suitable for soybean cultivation.

[0027] Beneficial effects:

[0028] This invention uses fermented soybean meal, pomegranate peel residue, eucommia leaf residue, compound microbial inoculum, and microbial activator as raw materials to prepare bio-organic fertilizer, wherein:

[0029] (1) After glucose complex enzymatic hydrolysis (cellulase + neutral protease) and anaerobic fermentation by lactic acid bacteria, phytic acid and antigenic proteins in soybean meal are degraded to form small molecule oligopeptides, lactic acid and other substances, which significantly improves nitrogen availability and provides carbon and nitrogen sources for functional microorganisms. At the same time, the lactic acid acidification environment inhibits the growth of putrefactive bacteria and ensures the safety of fermentation products.

[0030] (2) After steam explosion (1.2 MPa, 5-10 min), the lignin-cellulose structure of pomegranate peel residue disintegrates, increasing its specific surface area by 3 times. This, combined with natural chlorogenic acid and eucommia gum from eucommia leaf residue, forms an organic carrier with water-retaining and slow-release functions. Subsequent neutralization with lime water (pH 6.5-7.0) can desensitize the phytotoxicity of pomegranate peel tannins and simultaneously introduce calcium ions (Ca). 2+ This promotes the formation of soil aggregates.

[0031] (3) This invention screened two highly salt-tolerant growth-promoting microbial strains to form functional microorganisms. The salt-tolerant *Microbacterium malathiformes* used has high salt tolerance. The bioactive substances it secretes, such as glycine betaine and functional proteins, can induce the synthesis of plant cell osmotic regulators (proline, soluble sugars), enhance plant cell membrane stability, and alleviate salt stress damage. At the same time, the amylase and cellulase it produces can decompose lignocellulose substances (such as pomegranate peel residue), release soluble carbon sources, and dissolve fixed phosphorus and potassium in the soil by secreting organic acids, thereby improving nutrient availability. *Actinomyces madurae*, the strain produces indoleacetic acid, which directly promotes root elongation and lateral root development; at the same time, it secretes siderophores to chelate Fe in the soil. 3+ It promotes iron absorption in plants and alleviates iron deficiency chlorosis. The thioglucan and polyketide antibacterial substances produced by its metabolism can inhibit the reproduction of soil-borne pathogens such as Fusarium and Phytophthora, reducing the incidence of plant diseases. When the volume ratio of the two bacteria is 1:1, *A. chinensis* activates the enzyme production activity of salt-tolerant bacilli by secreting metabolites. The organic matter decomposed by the salt-tolerant bacilli (such as small molecule peptides from soybean meal) provides growth substrates for *A. chinensis*, forming a metabolic mutualistic relationship, which enables efficient fermentation of organic matter. The complex microbial community maintains high activity after composting and field application.

[0032] (4) This invention simultaneously adds a microbial activator composed of trehalose, chitosan, fish protein powder, potassium humate, and bayberry leaf extract. The flavonoids and polyphenols in bayberry leaves, combined with the high adsorption capacity of zeolite and zinc sulfate micro-fertilizer, form a product that combines antioxidant properties with slow-release of trace elements (Zn). 2+ The active functional units of trehalose significantly enhance the activity of plant antioxidant enzymes (SOD, POD). Trehalose, as a microbial stress protectant, enhances the survival rate of strains under drought or high-salt environments by stabilizing the phospholipid bilayer structure of cell membranes; chitosan strengthens systemic resistance by inducing the phenylpropanoid metabolic pathway in plants, activating the expression of peroxidase (POD) and chitinase genes; fish protein powder provides a variety of free amino acids, which can be directly used as a nitrogen source for plants and a growth promoter for microorganisms.

[0033] (5) In summary, the fertilizer of the present invention can effectively regulate and stabilize soil activity and promote crop growth. Under salt stress, it can effectively enhance the activity of plant stress-related enzymes, root vitality and carotenoid content in leaves, and improve the ability of soybean plants to resist salt stress adverse conditions. It has broad development potential and application value. Attached Figure Description

[0034] Figure 1 A graph showing the salt tolerance test results of salt-tolerant microbacteria compared to *Madula chinensis*.

[0035] Figure 2 A graph showing the drought resistance test results of salt-tolerant and alkali-tolerant Microbacterium tumefaciens compared to that of Madura chinensis.

[0036] Figure 3 The diagrams show the growth characteristics of salt-tolerant *Microbacterium salina* and *Madula chinensis*. A1-A3 represent the salt-tolerant *Microbacterium salina*, showing the formation of phosphate-solubilizing zones, potassium-solubilizing zones, and siderophores. B1-B3 represent *Madula chinensis*, showing the formation of phosphate-solubilizing zones, potassium-solubilizing zones, and siderophores. Detailed Implementation

[0037] The technical solution of the present invention will be further described below with reference to specific embodiments, but it is not limited thereto.

[0038] Example 1

[0039] A stress-resistant and growth-promoting stable fertilizer includes the following raw materials: fermented soybean meal, pomegranate peel residue, eucommia leaf residue, compound microbial inoculum, and microbial activator.

[0040] The composite microbial culture includes salt-tolerant microbacteria ( ) 嗜盐碱嗜盐杆菌 ) and Madura hygroscopica ( 奇贝岛马杜拉放线菌 The volume ratio of the two is 1:1.

[0041] The strain of the salt-tolerant *Microbacterium malathione* is CGMCC No. 1.4011, with an original deposit date of May 1, 2005; the strain of *Actinomyces madurae* is CGMCC No. 4.6236, with an original deposit date of September 3, 2008. Both strains were purchased from the conservation center and can be obtained through open commercial channels without the need for biological preservation.

[0042] The microbial activator includes trehalose, chitosan, fish protein powder, potassium humate, and bayberry leaf extract in a mass ratio of 1:1:15:3:2.

[0043] The preparation method of the bayberry leaf extract is as follows: collect bayberry branches and leaves, rinse with clean water to remove mud and sand, drain the water, crush the branches and leaves into 1-2cm fragments using a crusher, spread them out in a drying yard to air dry naturally for 3-5 days, and the moisture content of the crushed material is ≤15%; mix the crushed material with water at a ratio of 1:10, add 1% citric acid to the mixture, soak at a constant temperature of 60-80℃ for 10 hours, separate the solid and liquid to obtain the extract, and collect the bayberry leaf residue for later use; mix the extract with zeolite powder at a ratio of 1:3, stir to adsorb, spread out to air dry until the moisture content is ≤20%, add 3kg zinc sulfate per ton of adsorbate, mix evenly to obtain the bayberry leaf extract.

[0044] A method for preparing a stress-resistant and growth-promoting stable fertilizer includes the following preparation steps:

[0045] (1) Preparation of soybean meal fermentation product: Crush soybean meal to 60-80 mesh, add 5% of the soybean meal mass of a compound enzymatic hydrolysate containing glucose, enzymatically hydrolyze at 50-55℃ for 4 hours, then inoculate with lactic acid bacteria at 3% of the soybean meal mass, and anaerobic ferment at 35-37℃ for 72 hours to obtain soybean meal fermentation product.

[0046] (2) Treatment of pomegranate peel residue and eucommia leaf residue: After the pomegranate peel residue is exploded with steam at 1-1.2MPa for 5 minutes, it is mixed with eucommia leaf residue at a mass ratio of 2:1; then neutralized with lime water with a mass concentration of 1% to pH 6.5-7.0, dried and pulverized to 100 mesh to obtain a mixture of pomegranate peel residue and eucommia leaf residue.

[0047] (3) Preparation of compound microbial culture: After activating salt-tolerant Microbacterium maltophilum and Actinobacillus maculatus, they were inoculated into liquid culture medium and fermented at 28-36℃ for no less than 30 hours, with an effective viable count ≥10. 8 CFU / mL, then mix the two bacterial solutions at a volume ratio of 1:1, centrifuge to collect the bacterial cells, and prepare a compound microbial bacterial solution;

[0048] (4) Mix the filter residue of bayberry leaf extract, fermented soybean meal, pomegranate peel residue, and eucommia leaf residue in a mass ratio of 0.5:1:3 and crush them to a particle size of ≤5 mm to ensure uniform texture. Add rice husk powder or straw powder, adjust the carbon-nitrogen ratio of the mixture to 25:1, add water to adjust the moisture content to 60-65%, and obtain the mixed fermented material.

[0049] (5) Add compound microbial liquid at 1% of the total mass of the mixed fermentation material, spray it evenly into the mixed fermentation material, add microbial activator at 1% of the mass of the mixed fermentation material, mix thoroughly with a mixer, and carry out composting fermentation.

[0050] (6) Composting fermentation: In the early stage of composting, the temperature is raised to 55-65℃ and fermentation continues for 3-4 days; in the 4-10 days, the temperature is maintained at 50-60℃, and the pile is turned once a day to promote aerobic fermentation; in the 11-20 days, the temperature is naturally cooled down and fermented, and when the temperature drops below 40℃, the turning frequency is changed to once every 3 days to promote the formation of humus; the moisture content is maintained at 55-65% throughout the process, and water is added or the pile is turned to remove moisture when necessary; the material is dark brown and loose, odorless, and the pH is stable at 7.0-8.0. The seed germination index is ≥80% to complete the fermentation.

[0051] (7) Dry the decomposed material obtained in step (6) until the moisture content is ≤20%, crush it and sieve it, add 2-5% binder to granulate it, and control the particle size at 2-3 mm to obtain the finished fertilizer.

[0052] Step (1) The preparation method of the complex enzymatic hydrolysate containing glucose is as follows: add 5g glucose, 1g cellulase and 2g neutral protease to 3 liters of water, stir until dissolved, add 10mL white vinegar, and the pH is approximately 5.5.

[0053] Step (3) The liquid culture medium used for the salt-tolerant microbacterium tumefaciens consisted of: 10 g / L peptone, 5 g / L yeast extract, 1.5 g / L NaCl, and pH 7.0. Sterilization conditions: autoclaving at 121°C for 20 minutes.

[0054] Step (3) The liquid culture medium used for the *Mandula chinensis* consists of: peptone: 10 g / L, NaCl: 1.5 g / L, yeast extract: 5 g / L, glucose: 5 g / L, pH: 7.0-7.5 (adjusted with 1 mol / L NaOH or HCl), and sterilization conditions: autoclaving at 121℃ for 20 minutes.

[0055] Step (6) The composting adopts a windrow structure with an initial pile width of 1.5-2m and a pile height of 1.0-1.2m. Bamboo racks or wooden frames are laid at the bottom to ensure ventilation and control the temperature.

[0056] Example 2

[0057] A stress-resistant and growth-promoting stable fertilizer includes the following raw materials: fermented soybean meal, pomegranate peel residue, eucommia leaf residue, compound microbial inoculum, and microbial activator.

[0058] The composite microbial culture includes salt-tolerant microbacteria ( ) 嗜盐碱嗜盐杆菌 ) and Madura hygroscopica ( 奇贝岛马杜拉放线菌 The volume ratio of the two is 1:1.

[0059] The strain of the salt-tolerant *Microbacterium malathione* is CGMCC No. 1.4011, with an original deposit date of May 1, 2005; the strain of *Actinomyces madurae* is CGMCC No. 4.6236, with an original deposit date of September 3, 2008. Both strains were purchased from the conservation center and can be obtained through open commercial channels without the need for biological preservation.

[0060] The microbial activator includes trehalose, chitosan, fish protein powder, potassium humate, and bayberry leaf extract in a mass ratio of 1:1:15:3:2.

[0061] The preparation method of the bayberry leaf extract is as follows: collect bayberry branches and leaves, rinse with clean water to remove mud and sand, drain the water, crush the branches and leaves into 1-2cm fragments using a pulverizer, spread them out in a drying yard to air dry naturally for 3-5 days, and the moisture content of the crushed material is ≤15%; mix the crushed material with water at a ratio of 1:10, add 3% citric acid to the mixture, soak at a constant temperature of 60-80℃ for 15 hours, separate the solid and liquid to obtain the extract, and collect the bayberry leaf residue for later use; mix the extract with zeolite powder at a ratio of 1:3, stir to adsorb, spread out to air dry until the moisture content is ≤20%, add 5kg zinc sulfate per ton of adsorbate, mix evenly to obtain the bayberry leaf extract.

[0062] A method for preparing a stress-resistant and growth-promoting stable fertilizer includes the following preparation steps:

[0063] (1) Preparation of soybean meal fermentation product: Crush soybean meal to 60-80 mesh, add 5% of the soybean meal mass of a compound enzymatic hydrolysate containing glucose, enzymatically hydrolyze at 50-55℃ for 4 hours, then inoculate with lactic acid bacteria at 3% of the soybean meal mass, and anaerobic ferment at 35-37℃ for 72 hours to obtain soybean meal fermentation product.

[0064] (2) Treatment of pomegranate peel residue and eucommia leaf residue: After the pomegranate peel residue is exploded with steam at 1-1.2MPa for 10min, it is mixed with eucommia leaf residue at a mass ratio of 2:1; then neutralized with lime water with a mass concentration of 1% to pH 6.5-7.0, dried and pulverized to 100 mesh to obtain a mixture of pomegranate peel residue and eucommia leaf residue.

[0065] (3) Preparation of compound microbial culture: After activating salt-tolerant Microbacterium maltophilum and Actinobacillus maculatus, they were inoculated into liquid culture medium and fermented at 28-36℃ for no less than 30 hours, with an effective viable count ≥10. 8 CFU / mL, then mix the two bacterial solutions at a volume ratio of 1:1, centrifuge to collect the bacterial cells, and prepare a compound microbial bacterial solution;

[0066] (4) Mix the filter residue of bayberry leaf extract, fermented soybean meal, pomegranate peel residue, and eucommia leaf residue in a mass ratio of 0.5:1:3 and crush them to a particle size of ≤5 mm to ensure uniform texture. Add rice husk powder or straw powder, adjust the carbon-nitrogen ratio of the mixture to 30:1, add water to adjust the moisture content to 60-65%, and obtain the mixed fermented material.

[0067] (5) Add compound microbial liquid at 4% of the total mass of the mixed fermentation material, spray it evenly into the mixed fermentation material, add microbial activator at 1% of the mass of the mixed fermentation material, mix thoroughly with a mixer, and carry out composting fermentation.

[0068] (6) Composting fermentation: In the early stage of composting, the temperature is raised to 55-65℃ and fermentation continues for 3-4 days; in the 4-10 days, the temperature is maintained at 50-60℃, and the pile is turned once a day to promote aerobic fermentation; in the 11-20 days, the temperature is naturally cooled down and fermented, and when the temperature drops below 40℃, the turning frequency is changed to once every 3 days to promote the formation of humus; the moisture content is maintained at 55-65% throughout the process, and water is added or the pile is turned to remove moisture when necessary; the material is dark brown and loose, odorless, and the pH is stable at 7.0-8.0. The seed germination index is ≥80% to complete the fermentation.

[0069] (7) Dry the decomposed material obtained in step (6) until the moisture content is ≤20%, crush it and sieve it, add 2-5% binder to granulate it, and control the particle size at 2-3 mm to obtain the finished fertilizer.

[0070] Step (1) The preparation method of the complex enzymatic hydrolysate containing glucose is as follows: add 5g glucose, 1g cellulase and 2g neutral protease to 3 liters of water, stir until dissolved, add 10mL white vinegar, and the pH is approximately 5.5.

[0071] Step (3) The liquid culture medium used for the salt-tolerant microbacterium tumefaciens consisted of: 10 g / L peptone, 5 g / L yeast extract, 1.5 g / L NaCl, and pH 7.0. Sterilization conditions: autoclaving at 121°C for 20 minutes.

[0072] Step (3) The liquid culture medium used for the *Mandula chinensis* consists of: peptone: 10 g / L, NaCl: 1.5 g / L, yeast extract: 5 g / L, glucose: 5 g / L, pH: 7.0-7.5 (adjusted with 1 mol / L NaOH or HCl), and sterilization conditions: autoclaving at 121℃ for 20 minutes.

[0073] Step (6) The composting adopts a windrow structure with an initial pile width of 1.5-2m and a pile height of 1.0-1.2m. Bamboo racks or wooden frames are laid at the bottom to ensure ventilation and control the temperature.

[0074] Comparative Example 1

[0075] In this comparative example, except that only a single salt-tolerant and alkali-tolerant small bacilli were used in the composite microbial culture, the raw materials and process steps were the same as in Example 2. That is:

[0076] A stress-resistant and growth-promoting stable fertilizer includes the following raw materials: fermented soybean meal, pomegranate peel residue, Eucommia ulmoides leaf residue, microorganisms, and microbial activators.

[0077] The microorganism is a salt-tolerant bacillus.

[0078] A method for preparing a stress-resistant and growth-promoting stable fertilizer includes the following preparation steps:

[0079] (1) Preparation of soybean meal fermentation product: Crush soybean meal to 60-80 mesh, add 5% of the soybean meal mass of a compound enzymatic hydrolysate containing glucose, enzymatically hydrolyze at 50-55℃ for 4 hours, then inoculate with lactic acid bacteria at 3% of the soybean meal mass, and anaerobic ferment at 35-37℃ for 72 hours to obtain soybean meal fermentation product.

[0080] (2) Treatment of pomegranate peel residue and eucommia leaf residue: After the pomegranate peel residue is exploded with steam at 1-1.2MPa for 10min, it is mixed with eucommia leaf residue at a mass ratio of 2:1; then neutralized with lime water with a mass concentration of 1% to pH 6.5-7.0, dried and pulverized to 100 mesh to obtain a mixture of pomegranate peel residue and eucommia leaf residue.

[0081] (3) Microbial preparation: After activating the salt-tolerant and alkali-tolerant small bacilli, inoculate them into liquid culture medium and ferment them at 28-36℃ for no less than 30 hours until the effective viable count is ≥10. 8 CFU / mL, centrifuge to collect bacterial cells, and prepare microorganisms;

[0082] (4) Mix the filter residue of bayberry leaf extract, fermented soybean meal, pomegranate peel residue, and eucommia leaf residue in a mass ratio of 0.5:1:3 and crush them to a particle size of ≤5 mm to ensure uniform texture. Add rice husk powder or straw powder, adjust the carbon-nitrogen ratio of the mixture to 30:1, add water to adjust the moisture content to 60-65%, and obtain the mixed fermented material.

[0083] (5) Add microorganisms at 4% of the total mass of the mixed fermentation material, spray them evenly into the mixed fermentation material, add microbial activator at 1% of the mass of the mixed fermentation material, mix thoroughly with a mixer, and carry out composting fermentation.

[0084] (6) Composting fermentation: In the early stage of composting, the temperature is raised to 55-65℃ and fermentation continues for 3-4 days; in the 4-10 days, the temperature is maintained at 50-60℃, and the pile is turned once a day to promote aerobic fermentation; in the 11-20 days, the temperature is naturally cooled down and fermented, and when the temperature drops below 40℃, the turning frequency is changed to once every 3 days to promote the formation of humus; the moisture content is maintained at 55-65% throughout the process, and water is added or the pile is turned to remove moisture when necessary; the material is dark brown and loose, odorless, and the pH is stable at 7.0-8.0. The seed germination index is ≥80% to complete the fermentation.

[0085] (7) Dry the decomposed material obtained in step (6) until the moisture content is ≤20%, crush it and sieve it, add 2-5% binder to granulate it, and control the particle size at 2-3 mm to obtain the finished fertilizer.

[0086] Comparative Example 2

[0087] In this comparative example, except that only *Madula chinensis* was used in the composite microbial inoculum, the raw materials and process steps were the same as in Example 2. That is:

[0088] A stress-resistant and growth-promoting stable fertilizer includes the following raw materials: fermented soybean meal, pomegranate peel residue, Eucommia ulmoides leaf residue, microorganisms, and microbial activators.

[0089] The microorganism in question is *Madura chinensis*.

[0090] A method for preparing a stress-resistant and growth-promoting stable fertilizer includes the following preparation steps:

[0091] (1) Preparation of soybean meal fermentation product: Crush soybean meal to 60-80 mesh, add 5% of the soybean meal mass of a compound enzymatic hydrolysate containing glucose, enzymatically hydrolyze at 50-55℃ for 4 hours, then inoculate with lactic acid bacteria at 3% of the soybean meal mass, and anaerobic ferment at 35-37℃ for 72 hours to obtain soybean meal fermentation product.

[0092] (2) Treatment of pomegranate peel residue and eucommia leaf residue: After the pomegranate peel residue is exploded with steam at 1-1.2MPa for 10min, it is mixed with eucommia leaf residue at a mass ratio of 2:1; then neutralized with lime water with a mass concentration of 1% to pH 6.5-7.0, dried and pulverized to 100 mesh to obtain a mixture of pomegranate peel residue and eucommia leaf residue.

[0093] (3) Microbial preparation: After activating the *Madula maculata* actinomycete, it was inoculated into a liquid culture medium and fermented at 28-36℃ for no less than 30 hours until the effective viable count was ≥10. 8 CFU / mL, centrifuge to collect bacterial cells, and prepare microorganisms;

[0094] (4) Mix the filter residue of bayberry leaf extract, fermented soybean meal, pomegranate peel residue, and eucommia leaf residue in a mass ratio of 0.5:1:3 and crush them to a particle size of ≤5 mm to ensure uniform texture. Add rice husk powder or straw powder, adjust the carbon-nitrogen ratio of the mixture to 30:1, add water to adjust the moisture content to 60-65%, and obtain the mixed fermented material.

[0095] (5) Add microorganisms at 4% of the total mass of the mixed fermentation material, spray them evenly into the mixed fermentation material, add microbial activator at 1% of the mass of the mixed fermentation material, mix thoroughly with a mixer, and carry out composting fermentation.

[0096] (6) Composting fermentation: In the early stage of composting, the temperature is raised to 55-65℃ and fermentation continues for 3-4 days; in the 4-10 days, the temperature is maintained at 50-60℃, and the pile is turned once a day to promote aerobic fermentation; in the 11-20 days, the temperature is naturally cooled down and fermented, and when the temperature drops below 40℃, the turning frequency is changed to once every 3 days to promote the formation of humus; the moisture content is maintained at 55-65% throughout the process, and water is added or the pile is turned to remove moisture when necessary; the material is dark brown and loose, odorless, and the pH is stable at 7.0-8.0. The seed germination index is ≥80% to complete the fermentation.

[0097] The decomposed material obtained in step (6) is dried until the moisture content is ≤20%, crushed and sieved, and granulated with 2-5% binder. The particle size is controlled at 2-3 mm to obtain the finished fertilizer.

[0098] Comparative Example 3

[0099] In this comparative example, except for changing the strain type of salt-tolerant and alkali-tolerant Microbacterium in the composite microbial culture, the raw materials and process steps are the same as in Example 2. That is:

[0100] A stress-resistant and growth-promoting stable fertilizer includes the following raw materials: fermented soybean meal, pomegranate peel residue, eucommia leaf residue, compound microbial inoculum, and microbial activator.

[0101] The composite microbial culture includes salt-tolerant bacilli and Madura actinomycetes, with a volume ratio of 1:1.

[0102] The strain of the salt-tolerant *Microbacterium malathione* is CGMCC No. 1.15264, with an original deposit date of June 15, 2015; the strain of *Actinomyces madurae* is CGMCC No. 4.6236, with an original deposit date of September 3, 2008. Both strains were purchased from the preservation center.

[0103] Comparative Example 4

[0104] In this comparative example, except for the use of different actinomycetes in the composite microbial inoculum, the raw materials and process steps are the same as in Example 2. That is:

[0105] A stress-resistant and growth-promoting stable fertilizer includes the following raw materials: fermented soybean meal, pomegranate peel residue, eucommia leaf residue, compound microbial inoculum, and microbial activator.

[0106] The composite microbial culture includes salt-tolerant bacilli and Madura actinomycetes, with a volume ratio of 1:1.

[0107] The strain number of the salt-tolerant microbacterium is CGMCC No.1.4011, and the original deposit date is May 1, 2005; the *Madura chinensis* was purchased from Dingxiangtong-Nuoan Gene Technology (Wuhan) Co., Ltd.

[0108] Comparative Example 5

[0109] In this comparative example, except that the bayberry leaf extract was not used in the microbial activator, the raw materials and process steps were the same as in Example 2. That is:

[0110] A stress-resistant and growth-promoting stable fertilizer includes the following raw materials: fermented soybean meal, pomegranate peel residue, eucommia leaf residue, compound microbial inoculum, and microbial activator.

[0111] The microbial activator includes trehalose, chitosan, fish protein powder, and potassium humate in a mass ratio of 1:1:15:3.

[0112] A method for preparing a stress-resistant and growth-promoting stable fertilizer includes the following preparation steps:

[0113] (1) Preparation of soybean meal fermentation product: Crush soybean meal to 60-80 mesh, add 5% of the soybean meal mass of a compound enzymatic hydrolysate containing glucose, enzymatically hydrolyze at 50-55℃ for 4 hours, then inoculate with lactic acid bacteria at 3% of the soybean meal mass, and anaerobic ferment at 35-37℃ for 72 hours to obtain soybean meal fermentation product.

[0114] (2) Treatment of pomegranate peel residue and eucommia leaf residue: After the pomegranate peel residue is exploded with steam at 1-1.2MPa for 10min, it is mixed with eucommia leaf residue at a mass ratio of 2:1; then neutralized with lime water with a mass concentration of 1% to pH 6.5-7.0, dried and pulverized to 100 mesh to obtain a mixture of pomegranate peel residue and eucommia leaf residue.

[0115] (3) Preparation of compound microbial culture: After activating salt-tolerant bacilli and madura actinomycetes, they were inoculated into liquid culture medium and fermented at 28-36℃ for no less than 30 hours. The effective viable count was ≥108 CFU / mL. The two cultures were then mixed at a volume ratio of 1:1, and the cells were collected by centrifugation to prepare compound microbial culture.

[0116] (4) Mix the fermented soybean meal with pomegranate peel residue and eucommia leaf residue at a mass ratio of 1:3 and crush it to a particle size of ≤5 mm to ensure uniform texture. Add rice husk powder or straw powder, adjust the carbon-nitrogen ratio of the mixture to 30:1, add water to adjust the moisture content to 60-65%, and obtain the mixed fermented material.

[0117] (5) Add compound microbial liquid at 4% of the total mass of the mixed fermentation material, spray it evenly into the mixed fermentation material, add microbial activator at 1% of the mass of the mixed fermentation material, mix thoroughly with a mixer, and carry out composting fermentation.

[0118] (6) Composting fermentation: In the early stage of composting, the temperature is raised to 55-65℃ and fermentation continues for 3-4 days; in the 4-10 days, the temperature is maintained at 50-60℃, and the pile is turned once a day to promote aerobic fermentation; in the 11-20 days, the temperature is naturally cooled down and fermented, and when the temperature drops below 40℃, the turning frequency is changed to once every 3 days to promote the formation of humus; the moisture content is maintained at 55-65% throughout the process, and water is added or the pile is turned to remove moisture when necessary; the material is dark brown and loose, odorless, and the pH is stable at 7.0-8.0. The seed germination index is ≥80% to complete the fermentation.

[0119] (7) Dry the decomposed material obtained in step (6) until the moisture content is ≤20%, crush it and sieve it, add 2-5% binder to granulate it, and control the particle size at 2-3 mm to obtain the finished fertilizer.

[0120] Strain resistance test:

[0121] Salt tolerance tests of salt-tolerant *Microbacterium malathione* strain CGMCC No. 1.4011 and *Actinomyces madurae* strain CGMCC No. 4.6236:

[0122] LB liquid culture media containing NaCl concentrations of 0%, 0.5%, 1.0%, 2.0%, 3.0%, 5.0%, 7.0%, 9.0%, and 11.0% (w / v) were prepared. Salt-tolerant *Microbacterium malathione* and *Actinomyces madurae* were inoculated into the media with different NaCl concentrations, with each group replicated three times. The media were cultured at 28-36°C with shaking at 120 rpm until the logarithmic growth phase (OD50). 600 Approximately 1.0), measuring sample biomass (OD). 600 ). OD 600 The value is usually used to measure the concentration of bacterial culture, the relative bacterial concentration (OD). 600 The salt tolerance value (SQV) can objectively reflect the relative salt tolerance of a bacterial strain under salt stress. The test results for the two strains are as follows: Figure 1 As shown, the OD values ​​of the two bacterial strains at a NaCl concentration of 0.5% were... 600 The concentration was higher than that without NaCl, indicating that a suitable salt concentration is beneficial to the growth of the strain. As the concentration increased, the growth activity of the strain was inhibited, but both strains maintained activity at high concentrations below 7%, demonstrating that both strains have good salt tolerance.

[0123] Drought resistance tests of salt-tolerant *Microbacterium malathione* strain CGMCC No. 1.4011 and *Actinomyces madurae* strain CGMCC No. 4.6236:

[0124] Beef extract peptone liquid culture medium was prepared with PEG-6000 concentrations of 0%, 7.5%, 9.0%, 10.5%, 12.0%, 13.5%, and 15.0%. Salt-tolerant *Microbacterium malathione* and *Actinomyces madurae* were inoculated into the different concentrations of the medium, with each group in triplicate. The cultures were incubated at 28-36°C with shaking at 120 rpm until the logarithmic growth phase (OD50). 600 Approximately 1.0), measuring sample biomass (OD). 600 ).

[0125] The drought resistance of the strain was studied by setting different concentrations of PEG-6000 to simulate drought. The results are as follows: Figure 2As shown, salt-tolerant bacilli exhibit stronger drought resistance compared to Madura chinensis.

[0126] Determination of growth-promoting characteristics of strains:

[0127] Following the method of Huang Chen et al. (Huang Chen, Yang Kaiyuan, Gao Peng, Liang Yinping, Han Lingjuan, Zhao Xiang. Screening, identification and characteristic study of phosphate-solubilizing bacteria in the rhizosphere of Lespedeza dauricum. Acta Grasslandica Sinica, 2022, 30(9):2345-2355.), we observed whether a transparent phosphate-solubilizing ring appeared around the colony to determine the phosphate-solubilizing ability of the isolates; we inoculated the bacterial cake upside down in the center of a CAS medium plate and incubated it at 28-36℃ for 5-7 days, observing whether a yellow halo appeared on the test plate, and the larger the diameter of the halo, the stronger the siderophore secretion ability (Yu Xinyan. Study on the regulatory mechanism of siderophore synthesis in high-siderophore-producing Pseudomonas HYS [D]. Wuhan: Wuhan University, 2014.); The IAA production capacity of the target isolates was determined by Salkowski's colorimetric method. For specific methods, please refer to the following reference: (Wang Xixiang, Xu Kun, Zhang Dongmei, Du Binghai, Yang Qianqian, Hu Xiuna, Ding Yanqin. Preliminary identification of five ginger growth-promoting bacteria and determination of IAA production and antibacterial ability. Shandong Agricultural Sciences, 2015, 47(1):36-40,46.).

[0128] Testing revealed that both *Salmonella haematobacterium* and *Actinomyces madurae* could decompose inorganic phosphorus and insoluble potassium salts in the culture medium, producing phosphate-solubilizing and potassium-solubilizing zones with diameters of 36.3 mm and 33.3 mm, respectively. The potassium-solubilizing zones had diameters of 22.7 mm and 20.7 mm, respectively. Both isolates produced yellow halos on the CAS detection medium, indicating their siderophore-producing activity (yellow halo diameters were 20.4 mm and 16.6 mm). The measured OD values ​​of the samples were... 530 The values ​​were 0.181 and 0.192. Using Salkowski's colorimetric method and referring to the IAA standard curve (y = 0.0142x - 0.0076), the calculated IAA yield was 13.3 μg·mL. -1 14.1 μg·mL -1 Both plants have good growth-promoting properties. Figure 3 The diagrams show the growth characteristics of salt-tolerant *Microbacterium salina* and *Madula chinensis*. A1-A3 represent the salt-tolerant *Microbacterium salina*, showing the formation of phosphate-solubilizing zones, potassium-solubilizing zones, and siderophores. B1-B3 represent *Madula chinensis*, showing the formation of phosphate-solubilizing zones, potassium-solubilizing zones, and siderophores.

[0129] Pot experiment:

[0130] The experimental setup and treatment groups are as follows:

[0131] A1: Blank control, no processing performed;

[0132] A2: Salt treatment;

[0133] A3: Salt treatment + application of fertilizer from Example 1, at a rate of 200 kg / mu;

[0134] A4: Salt treatment + application of fertilizer from Example 2, at a rate of 200 kg / mu;

[0135] A5: Salt treatment + application of fertilizer according to Comparative Ratio 1, at a rate of 200 kg / mu;

[0136] A6: Salt treatment + application of fertilizer according to Comparative Ratio 2, at a rate of 200 kg / mu;

[0137] A7: Salt treatment + application of fertilizer according to Comparative Ratio 3, at a rate of 200 kg / mu;

[0138] A8: Salt treatment + application of this comparative ratio 4 fertilizer, at a rate of 200 kg / mu;

[0139] A9: Salt treatment + application of this comparative fertilizer at 200 kg / mu; 15 replicates per treatment.

[0140] The specific operating method is as follows: Sifted soil is poured into mushroom bags at a rate of 600 g per bag. The soil is thoroughly watered with distilled water beforehand and allowed to evaporate naturally for three days. Soybean seeds are first disinfected with 75% ethanol for 30 seconds, then immediately washed twice with water, followed by shaking with 50% sodium hypochlorite solution for 3 minutes, and then rinsed 5-6 times with sterile water. Soybean seeds with wrinkled seed coats are picked up with tweezers and placed on 2% water agar plates for germination treatment. When the soybean sprouts reach 2-3 cm in length, they are transferred to mushroom bags for further growth. The plants are grown in an artificial climate chamber with a growth environment of 14 hours of light / 10 hours of darkness, 80% relative humidity, 25℃ temperature, and 190 µmol / m² effective light radiation. -2 s -1 Water with distilled water every three days during the growing season. After the first true leaf of the soybean unfolds, apply salt. Add 30 mL of 200 mM NaCl solution to each plant for the salt treatment, and apply an equal volume of sterile distilled water to the blank control. Mix the fertilizer with the planting soil at a depth of 0-20 cm before transplanting.

[0141] Soybean plants that have reached maturity were collected, and their plant height, stem diameter, dry weight, and chlorophyll content were measured. The specific procedures are as follows:

[0142] The plant height was measured by the distance from the cotyledon scar to the highest point of the leaf. The stem diameter below the cotyledon scar was measured using vernier calipers. Soybean stems, leaves, and roots were collected and placed in an oven at 105℃ for 5 minutes to kill the green, then dried at 85℃ for 4 hours. The samples dried to constant weight were weighed and recorded as dry weight. Three plants were used for each treatment. The SPAD value of soybean leaves was determined using a SPAD-TYS-B chlorophyll meter.

[0143] Determination of the activity of antioxidant enzymes in plant roots: MDA content, SOD, POD and CAT activity were determined using a kit method.

[0144] Soil enzyme activity assays: Catalase was determined by potassium permanganate titration; alkaline phosphatase, urease, and soil sucrase were determined using Solarbio Biochemical Kits (Solarbio Technology Co., Ltd., Beijing, China).

[0145] Table 1 Results of plant growth experiment

[0146]

[0147] Examples 1-2 maintained high growth indicators (plant height 81.2 cm, chlorophyll 56.7 SPAD) under salt stress, representing increases of 6.3-10.1% compared to Comparative Examples 1-2 (using only a single strain). Comparative Examples 3-4, which changed the strain species, also showed a decrease. This indicates that the specific strain selected in this invention, a 1:1 combination of salt-tolerant *Microbacterium malathiformes* and *Actinomyces madurae*, can significantly promote symbiotic microbial community and metabolic synergy, enhancing the salt tolerance of soybeans. Comparing Example 2 with Comparative Example 5 (without *Myrica rubra* leaf extract), chlorophyll content decreased by 16.2%. Flavonoids (such as myricetin) in *Myrica rubra* leaves can scavenge ROS and reduce chloroplast damage (SPAD reflects leaf photosynthetic capacity), while zinc sulfate enhances the activity of zinc-dependent chlorophyll synthase.

[0148] Table 2. Oxidative stress data in soybean root systems.

[0149]

[0150] Table 3 Effects on soil enzyme activity

[0151]

[0152] By comparing the experimental and control group data, the positive effects of the specific bacterial strain combination and bayberry leaf extract on soybean growth and antioxidant enzyme activity were clearly observed, further verifying the significant effect of this combination in improving soybean salt tolerance. It also played a crucial role in stabilizing and regulating soil biological activity, significantly increasing root enzyme activity such as alkaline phosphatase and urease, enhancing soil nutrient cycling, and optimizing the root microenvironment, thereby comprehensively improving the survival ability of soybeans under salt stress. Furthermore, the experiment showed that this combination can significantly reduce MDA content and increase the activity of antioxidant enzymes such as SOD and POD, effectively alleviating oxidative damage to soybean cells caused by salt stress, further ensuring the healthy and stable growth of soybeans.

[0153] It should be noted that the above embodiments are merely some preferred embodiments of the present invention, and not all embodiments. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of the present invention.

Claims

1. A stress-resistant and growth-promoting stable fertilizer, characterized in that, The raw materials include: fermented soybean meal, pomegranate peel residue, eucommia leaf residue, compound microbial inoculum, and microbial activator; the compound microbial inoculum includes salt-tolerant *Microbacterium malathione* and *Actinomyces madurae*, with a volume ratio of 1:1; the strain number of the salt-tolerant *Microbacterium malathione* is CGMCC No. 1.4011, and the strain number of *Actinomyces madurae* is CGMCC No. 4.6236; The microbial activator includes trehalose, chitosan, fish protein powder, potassium humate, and bayberry leaf extract in a mass ratio of 1:1:15:3:2; The preparation method of the bayberry leaf extract is as follows: collect bayberry branches and leaves, rinse with clean water to remove mud and sand, drain the water, crush the branches and leaves into 1-2cm fragments using a pulverizer, spread them on a drying ground to air dry naturally for 3-5 days, and the moisture content of the crushed material is ≤15%; mix the crushed material with water at a ratio of 1:10, add 1%-3% citric acid to the mixture, soak at a constant temperature of 60-80℃ for 10-15 hours, separate the solid and liquid to obtain the extract, and collect the bayberry leaf filter residue for later use; mix the extract with zeolite powder at a ratio of 1:3, stir and adsorb, spread out and air dry until the moisture content is ≤20%, add 3-5kg zinc sulfate per ton of adsorbate, mix evenly to obtain the bayberry leaf extract.

2. A method for preparing the stress-resistant and growth-promoting stable fertilizer according to claim 1, characterized in that, The preparation steps include the following: (1) Preparation of soybean meal fermentation product: Crush soybean meal to 60-80 mesh, add 5% of the soybean meal mass of a compound enzymatic hydrolysate containing glucose, enzymatically hydrolyze at 50-55℃ for 4 hours, then inoculate with lactic acid bacteria at 3% of the soybean meal mass, and anaerobic ferment at 35-37℃ for 72 hours to obtain soybean meal fermentation product. (2) Treatment of pomegranate peel residue and eucommia leaf residue: After the pomegranate peel residue is exploded with steam at 1-1.2MPa for 5-10 minutes, it is mixed with eucommia leaf residue at a mass ratio of 2:1; then neutralized with lime water with a mass concentration of 1% to pH 6.5-7.0, dried and pulverized to 100 mesh to obtain a mixture of pomegranate peel residue and eucommia leaf residue. (3) Preparation of compound microbial culture: After activating salt-tolerant Microbacterium maltophilum and Actinobacillus maculatus, they were inoculated into liquid culture medium and fermented at 28-36℃ for no less than 30 hours, with an effective viable count ≥10. 8 CFU / mL, then mix the two bacterial solutions at a volume ratio of 1:1, centrifuge to collect the bacterial cells, and prepare a compound microbial bacterial solution; (4) Mix the filter residue of bayberry leaf extract, fermented soybean meal, pomegranate peel residue, and eucommia leaf residue in a mass ratio of 0.5:1:3 and crush them to a particle size of ≤5 mm to ensure uniform texture. Add rice husk powder or straw powder, adjust the carbon-nitrogen ratio of the mixture to 25-30:1, add water to adjust the moisture content to 60-65%, and obtain the mixed fermented material. (5) Add compound microbial liquid at 1-4% of the total mass of the mixed fermentation material, spray it evenly into the mixed fermentation material, add microbial activator at 1% of the mass of the mixed fermentation material, mix thoroughly with a mixer, and carry out composting fermentation. (6) Composting fermentation: In the early stage of composting, the temperature is raised to 55-65℃ and fermentation continues for 3-4 days; in the 4-10 days, the temperature is maintained at 50-60℃, and the pile is turned once a day to promote aerobic fermentation; in the 11-20 days, the temperature is naturally cooled down and fermented, and when the temperature drops below 40℃, the turning frequency is changed to once every 3 days to promote the formation of humus; the moisture content is maintained at 55-65% throughout the process, and water is added or the pile is turned to remove moisture when necessary; the material is dark brown and loose, odorless, and the pH is stable at 7.0-8.

0. The seed germination index is ≥80% to complete the fermentation. (7) Dry the decomposed material obtained in step (6) until the moisture content is ≤20%, crush it and sieve it, add 2-5% binder to granulate it, and control the particle size at 2-3 mm to obtain the finished fertilizer.

3. The method for preparing the stress-resistant and growth-promoting stable fertilizer according to claim 2, characterized in that, Step (1) The preparation method of the complex enzymatic hydrolysate containing glucose is as follows: add 5g glucose, 1g cellulase and 2g neutral protease to 3 liters of water, stir until dissolved, add 10mL white vinegar, and the pH is approximately 5.

5.

4. The method for preparing the stress-resistant and growth-promoting stable fertilizer according to claim 2, characterized in that, Step (6) The composting adopts a windrow structure with an initial pile width of 1.5-2m and a pile height of 1.0-1.2m. Bamboo racks or wooden frames are laid at the bottom to ensure ventilation and control the temperature.

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