A mutant nad(p)h-quinone dehydrogenase, engineered microorganisms and uses thereof

By mutating the 27th amino acid of Corynebacterium glutamicum NAD(P)H-quinone dehydrogenase to aspartic acid or glycine, the problem of insufficient threonine production performance in the existing technology was solved, and a significant improvement in threonine yield and conversion rate was achieved.

CN122168548APending Publication Date: 2026-06-09MEIHUA BIOTECH LANGFANG CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
MEIHUA BIOTECH LANGFANG CO LTD
Filing Date
2024-12-06
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

In the existing technology, there are still shortcomings in improving the production performance of L-threonine by Corynebacterium glutamicum, and there is a lack of effective genetic engineering targets and methods. There are also few studies on the impact of NAD(P)H-quinone dehydrogenase mutants on amino acid production.

Method used

By mutating specific amino acids in the NAD(P)H-quinone dehydrogenase of Corynebacterium glutamicum to make its 27th amino acid aspartic acid or glycine, a mutant NAD(P)H-quinone dehydrogenase was developed to improve the synthesis and conversion rate of threonine.

Benefits of technology

It significantly improved the yield and conversion rate of threonine, provided a new genetic engineering strategy, and enhanced the production performance of the strain.

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Abstract

This invention relates to the field of genetic engineering technology, specifically to a mutant NAD(P)H-quinone dehydrogenase, engineered microorganisms, and their applications. The mutant NAD(P)H-quinone dehydrogenase provided by this invention can effectively enhance the threonine production capacity of bacterial strains. Introducing this mutant NAD(P)H-quinone dehydrogenase into bacterial strains significantly improves the threonine yield and conversion rate. This mutant NAD(P)H-quinone dehydrogenase provides a new strategy and method for improving the performance of amino acid-producing strains such as threonine, and has promising application prospects in amino acid strain modification.
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