Composition with function of brightening light color and skin care product containing composition

Abstract

The invention relates to a composition with a function of brightening light color and a skin care product containing the composition. The composition is prepared from rice fermentation liquor, 3-type recombinant collagen, 17-type recombinant collagen and hypnea elongata extract according to the mass ratio of (0.1 to 80): (0.1 to 50): (0.1 to 50): (0.1 to 30). Compared with the prior art, the composition disclosed by the invention is complementary in action mechanism, and has the effects of promoting collagen synthesis, removing yellowness and darkness, lightening skin color and uniformizing skin color through combined application.

Description

Technical Field

[0001] The invention relates to the field of cosmetics, in particular to a composition with the function of brightening and lightening pigmentation and a skin care product containing the composition. Background Art

[0002] Skin pigmentation, a common skin phenomenon, is indeed a common problem that troubles many people. Whether it is sun spots that gradually accumulate in the sun, or spots caused by aging, endocrine changes, genetic factors or certain health conditions, the skin is no longer as uniform and translucent as it was when it first appeared, and unpleasant pigmentation appears instead. Whether it is spots and dullness on the face, or uneven skin color in other parts of the body, it may affect people's self-confidence and appearance.

[0003] Many whitening products have appeared in the prior art. For example, patent application CN202110528985.5 discloses a composition with whitening and anti-aging functions and a preparation method, comprising the following components in parts by weight: collagen, 0.08-0.15 parts; loofah extract, 4.5-6.5 parts; alnus spatholobi proanthocyanidins, 1.5-2.5 parts; niacinamide, 0.8-1.2 parts; sodium oligohyaluronate, 1.8-2.2 parts; yeast extract, 10-16 parts; polypeptide mixture, 6-10 parts; α-glucan oligosaccharide, 2-3 parts; laurocapram, 1.5-2.5 parts; ethanol, 1-2 parts; butylene glycol, 18-22 parts; loofah water, 10-15 parts; deionized water, 10-15 parts. The preparation method is to mix the above components in proportion to obtain the final product. This technology improves the whitening and anti-aging effects from the perspective of inhibiting melanin production, reducing free radical damage to the skin, and improving skin elasticity and moisture. Currently, the only way to combat pigmentation with commercially available functional cosmetics is to use whitening agents or exfoliating products, but they are irritating to the skin or have an overall effect on the skin tone because they do not specifically act on the spots themselves, but generally whiten the skin as a whole. Therefore, there is an urgent need to provide a skin care product that can lighten pigmentation and even out skin tone, which can improve or reverse this condition to a certain extent and make the skin glow again. Summary of the invention

[0004] The purpose of the present invention is to overcome the defects of the above-mentioned prior art and provide a composition with the function of brightening and lightening pigmentation and a skin care product containing the composition.

[0005] The object of the present invention can be achieved by the following technical scheme: a composition with the function of brightening and relieving pigmentation, comprising rice fermentation liquid, type 3 recombinant collagen, type 17 recombinant collagen and elongated seaweed extract in a mass ratio of (0.1-80): (0.1-50): (0.1-50): (0.1-30).

[0006] Furthermore, the mass ratio of the rice fermentation broth, type 3 recombinant collagen, type 17 recombinant collagen and the Laminaria elongata extract is (1-14): (1-14): (1-18): (1-15).

[0007] Furthermore, the mass ratio of the rice fermentation broth, type 3 recombinant collagen, type 17 recombinant collagen and the Laminaria elongata extract is (1-10): (1-10): (1-10): (1-10).

[0008] The present invention also provides a skin care product containing the composition, wherein the weight percentage of the composition in the skin care product is 5-50%. Preferably, the weight percentage of the composition in the skin care product is 10-30%.

[0009] Furthermore, the skin care products include toner, softener, firming lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, facial mask, powder, body lotion, body cream, body oil, body milk or body essence.

[0010] Furthermore, the skin care product also contains 50-95% of matrix excipients by mass. Preferably, the matrix excipients account for 70-90% of the skin care product.

[0011] Furthermore, the matrix auxiliary materials include one or more of surfactants, thickeners, conditioners, moisturizers, preservatives, chelating agents, flavors, and deionized water.

[0012] Furthermore, the skin care product is preferably an essence, which comprises the following components in percentage by weight:

[0013] Phase A auxiliary materials include:

[0014] water to 100;

[0015] Xanthan gum 0.01~0.1;

[0016] Carbomer 0.05~1;

[0017] Sodium hyaluronate 0.01~0.8;

[0018] Allantoin 0.1~2;

[0019] Glycerol 1-10;

[0020] Phase B auxiliary materials include:

[0021] Jojoba oil 1-3;

[0022] Cetyl alcohol 0.1~1;

[0023] Cetearyl Glucoside (and) Cetearyl Alcohol 0.1~1;

[0024] Squalane 0.1~2;

[0025] Phase C auxiliary materials include:

[0026] Arginine 0.01~2;

[0027] Phase D auxiliary materials include:

[0028] (Daily use) essence 0.01~1;

[0029] Phenoxyethanol 0.1~0.5;

[0030] Ethylhexylglycerin 0.01~0.5;

[0031] Phase E materials include:

[0032] Rice fermentation liquid 1-14;

[0033] Type 3 recombinant collagen 1-14;

[0034] Recombinant collagen types 1-17;

[0035] 1-15 ml of elongated seaweed extract.

[0036] The essence is prepared by the following method:

[0037] a. Water phase: Place water in a container with a stirring device and stir, slowly disperse carbomer in the water, fully wet and mix xanthan gum, sodium hyaluronate, allantoin and glycerin, add them into the water, stir until completely dissolved, and heat to 75-80°C;

[0038] b. Oil phase; mix cetyl alcohol, cetearyl glucoside (and) cetearyl alcohol, squalane, and jojoba oil and heat to 75-80°C, stirring until completely dissolved;

[0039] c. Emulsification: Add the oil phase obtained in step b to the aqueous phase obtained in step a and homogenize at 2000-4000rpm for 4-6 minutes;

[0040] d. Add arginine, stir at 250-350 rpm for 2-4 min, and cool to 40-50°C with stirring;

[0041] e. Add (daily use) flavor, phenoxyethanol, ethylhexylglycerin, stir at 250-350rpm for 2-4min, then homogenize at 2000-4000rpm for 2-4min;

[0042] f. After cooling to 35-38°C, add rice fermentation liquid, type 3 recombinant collagen, type 17 recombinant collagen and seaweed extract, stir at 250-350rpm for 4-6min, and then homogenize at 2000-4000rpm for 4-6 minutes to obtain the essence.

[0043] Compared with the prior art, the present invention has the following beneficial effects:

[0044] 1. The present invention uses rice fermentation liquid, type 3 recombinant collagen, type 17 recombinant collagen and elongated seaweed extract in a specific ratio for synergistic effect, wherein the rice fermentation liquid contains more than 90 natural skin care ingredients such as amino acids, sugars, fruit acids, polyamines, nucleic acids, etc., has excellent skin affinity, super permeability and moisturizing and anti-aging properties, and provides multiple nutrients to the skin; elongated seaweed can stimulate the decomposition and metabolism of melanin, restore the control of skin pigmentation, and has a significant improvement effect on pigment deposition diseases such as spots and uneven skin color, thereby helping to remove cells containing melanin and reduce pigment deposition; the recombinant human collagen in the present invention is screened from 483-512 amino acid fragments, which are 100% homologous to the human body, and the collagen network formed after self-assembly of triple helices is consistent with the gap of the human body, has excellent transdermal effect, is stable and lasting, wherein type 3 collagen is a small molecule protein, can be absorbed through the skin, and directly supplements type 3 collagen in the entire skin layer; type 17 collagen is a transmembrane protein, and this fragment has a unique arginine pin and mortise and tenon structure, and the structure stabilizes and acts on the dermis. The combination of the two can directly supplement collagen to the skin. Due to the improvement of the cell microenvironment, the metabolism of melanocytes is promoted, thereby accelerating the metabolism and elimination of melanin. On the other hand, the improvement of the cell microenvironment promotes the metabolism of fibroblasts and promotes the expression of other collagens. Ultimately, direct collagen supplementation of the entire skin layer is achieved, and other collagens are regenerated.

[0045] The four active ingredients stimulate the decomposition of melanin, the replenishment and regeneration of collagen through better nutritional supplementation, accelerate the metabolism of melanocytes as a whole, give full play to the synergistic effect, and achieve better whitening and pigmentation effects.

[0046] 2. The composition of the present invention can play a synergistic role and complement each other in the mechanism of action. The combined application can promote collagen synthesis, remove yellowness and dullness, lighten skin pigmentation, and even out skin color, thereby achieving a better whitening and pigmentation effect. DETAILED DESCRIPTION

[0047] The present invention is described in detail below with reference to specific embodiments.

[0048] The present invention selects a specific ratio of rice fermentation liquid, type 3 recombinant collagen, type 17 recombinant collagen and elongated seaweed extract for combined use, which has a synergistic effect, has the effects of promoting collagen synthesis, removing yellowness and dullness, lightening skin color, and even skin color. The specific description is as follows:

[0049] 1. Rice fermentation liquid

[0050] Rice fermentation liquid is an active extract obtained by precision fermentation of high-purity and high-activity yeast species Saccharomyces veronae with high-quality rice at 40° north latitude. Rice fermentation liquid contains more than 90 natural skin care ingredients such as amino acids, sugars, fruit acids, polyamines, nucleic acids, etc., with excellent skin affinity, super permeability and moisturizing and anti-aging properties. Alpha-ketoglutaric acid (Alpha-KG) rich in rice fermentation liquid is an important component for the synthesis of ATP (cell energy factor). When cells have sufficient ATP supply, the synthesis and metabolism of their nutrients can proceed normally, and the vitality of cells can be repaired and maintained, thereby achieving skin renewal and youthfulness from the level of cell and energy metabolism. At the same time, it can also promote collagen synthesis, regulate microecology, and promote the production of various ingredients that enhance skin structure such as ceramides, desmosomes and tight junction proteins, achieving the effects of skin repair, toughness, self-repair and renewal from the stratum corneum and granular layer in multiple dimensions.

[0051] In a preferred embodiment, the rice fermentation broth is prepared by the following method:

[0052] (1) adding water to rice powder, heating, stirring, and becoming 30% rice milk, adding β-glucanase to the rice milk, the addition amount of the enzyme being 0.005% of the mass of the rice milk, and the enzyme activity being 200 U / g, performing enzymolysis, and obtaining a rice milk enzymolyzate;

[0053] (2) inoculating yeast strain Saccharomyces veronae into rice milk enzymatic hydrolysate, and fermenting under the following conditions: fermentation temperature of 35° C., fermentation time of 36 hours, pH value of the fermentation process being controlled between 6 and 6.5, and obtaining rice fermentation liquid after fermentation;

[0054] (3) The fermented rice fermentation liquid is concentrated 10 times by reverse osmosis technology to efficiently enrich the active substances, and then heated, sterilized, cooled and filtered to obtain the concentrated rice fermentation liquid, i.e., the yeast / rice fermentation product filtrate.

[0055] Recombinant collagen type 2 and 3

[0056] Type 3 recombinant collagen is a selected human type 3 collagen gene chip without non-human amino acid sequence modification. It has a clear triple helix structure. Due to its unique molecular structure and rich hydrophilic amino acid residues, it exhibits strong hydrophilic properties and is very suitable for the adhesion and growth of fibroblasts. It can also effectively prevent the conversion of tyrosine to dopaquinone by inhibiting the activity of tyrosinase. Tyrosinase is the main enzyme that catalyzes this reaction, and the inhibitory effect of recombinant human collagen directly leads to the obstruction of the melanin synthesis process, thereby significantly reducing the phenomenon of pigmentation, and has a significant improvement effect on pigment deposition diseases such as spots and uneven skin color.

[0057] In one embodiment of the present invention, the type 3 recombinant collagen is a commercially available product of Shanxi Jinbo Biopharmaceutical Co., Ltd.

[0058] 3. Recombinant collagen type 17

[0059] Type 17 recombinant collagen is a transmembrane protein that is mainly found at the junction of the dermis and epidermis of the skin. It is an important bridge connecting the dermis and epidermis. Although its content in the skin is relatively small, its role is crucial. Type 17 collagen is composed of three polypeptide chains, namely α1 (XVII), α2 (XVII) and α3 (XVII). These polypeptide chains are arranged in an α-helical structure, forming a certain degree of extensibility and stability. In addition, the amino terminus (N-terminus) of type 17 collagen is located in the cytoplasm, and the carboxyl terminus (C-terminus) is located in the extracellular matrix. This transmembrane structure gives it a unique connection and support role in the skin structure. Therefore, maintaining sufficient type 17 collagen in the skin is of great significance for maintaining skin health and delaying aging.

[0060] In one embodiment of the present invention, the type 17 recombinant collagen is a commercially available product of Shanxi Jinbo Biopharmaceutical Co., Ltd.

[0061] 4. Seaweed extract

[0062] Seagrass extract is a natural extract from large seaweed. In skin metabolism, SDF-1 is a key protein responsible for the communication between fibroblasts and melanocytes in the skin. One of its roles is to keep tyrosinase in a controlled state, ultimately regulating the pigmentation process. As we age, the production of SDF-1 decreases dramatically, which causes melanocytes to over-secrete melanin, forming dark spots on the surface of the skin. Seagrass produces a type of biopolyphenol, more specifically brown algae polyphenols (phloroglucinol oligomers), and these specific molecules can reactivate the expression of SDF-1, stimulate the catabolism of melanin, and restore control of skin pigmentation, thereby helping to remove cells containing melanin and reduce pigmentation.

[0063] In one embodiment of the present invention, the extract of the seaweed is a commercially available product of Givaudan, B-Lightyl TM .

[0064] 5. Synergy

[0065] The invention adopts rice fermentation liquid, type 3 recombinant collagen, type 17 recombinant collagen and elongated seaweed extract as raw materials and combines them in a suitable weight ratio. The four active ingredients can play a synergistic role and complement each other in the mechanism of action. The combined application has the effects of promoting collagen synthesis, removing yellowness and dullness, lightening skin color, and even skin color, thereby achieving a better whitening and lightening effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0066] Figure 1 This is a Visia skin spot diagram using the product obtained in Example 1.

[0068] The following is a detailed description through specific examples. The raw materials and detection means involved in the present invention are all commonly used raw materials and detection means in the art unless otherwise specified, and the amounts used in the present invention are all measured by mass unless otherwise specified.

[0069] Example 1

[0070] The whitening and pigmentation-reducing composition of this embodiment comprises the following ingredients in a weight ratio: rice fermentation liquid: type 3 recombinant collagen: type 17 recombinant collagen: Laminaria elongata extract = 25:25:25:25.

[0071] A skin care product comprising the above composition, wherein the mass percentage of the above composition in the skin care product is 10%, and further comprising a skin care product external dosage form matrix, wherein the skin care product external dosage form matrix can be conventionally selected by technicians in this field according to actual needs, and the skin care product can be prepared according to conventional methods.

[0072] Example 2

[0073] The whitening and pigmentation-reducing composition of this embodiment comprises the following ingredients in a weight ratio: rice fermentation liquid: type 3 recombinant collagen: type 17 recombinant collagen: elongated seaweed extract = 70:5:15:10.

[0074] A skin care product comprising the above composition, wherein the mass percentage of the above composition in the skin care product is 20%, and further comprising a skin care product external dosage form matrix. The skin care product external dosage form matrix can be conventionally selected by technicians in this field according to actual needs, and the skin care product can be prepared according to conventional methods.

[0075] Example 3

[0076] The whitening and pigmentation-reducing composition of this embodiment comprises the following ingredients in a weight ratio: rice fermentation liquid: type 3 recombinant collagen: type 17 recombinant collagen: elongated seaweed extract = 30:45:5:20.

[0077] A skin care product comprising the above composition, wherein the mass percentage of the above composition in the skin care product is 30%, and further comprising a skin care product external dosage form matrix. The skin care product external dosage form matrix can be conventionally selected by technicians in this field according to actual needs, and the skin care product can be prepared according to conventional methods.

[0078] Example 4

[0079] The whitening and pigmentation-reducing composition of this embodiment comprises the following ingredients in a weight ratio: rice fermentation liquid: type 3 recombinant collagen: type 17 recombinant collagen: elongated seaweed extract = 15:35:45:5.

[0080] A skin care product comprising the above composition, wherein the mass percentage of the above composition in the skin care product is 40%, and further comprising a skin care product external dosage form matrix. The skin care product external dosage form matrix can be conventionally selected by technicians in this field according to actual needs, and the skin care product can be prepared according to conventional methods.

[0081] Example 5

[0082] The whitening and pigmentation-reducing composition of this embodiment comprises the following ingredients in a weight ratio: rice fermentation liquid: type 3 recombinant collagen: type 17 recombinant collagen: elongated seaweed extract = 20:15:35:30.

[0083] A skin care product comprising the above composition, wherein the mass percentage of the above composition in the skin care product is 50%, and further comprising a skin care product external dosage form matrix. The skin care product external dosage form matrix can be conventionally selected by technicians in this field according to actual needs, and the skin care product can be prepared according to conventional methods.

[0084] Comparative Examples 1-8

[0085] Comparative Example 1 is the same as Example 1 except that it does not contain rice fermentation broth, Comparative Example 2 is the same as Example 1 except that it does not contain type 3 recombinant collagen, Comparative Example 3 is the same as Example 1 except that it does not contain type 17 recombinant collagen, Comparative Example 4 is the same as Example 1 except that it does not contain elongated seaweed strip extract, Comparative Example 5 is the same as Example 1 except that it contains only rice fermentation broth, Comparative Example 6 is the same as Example 1 except that it only contains type 3 recombinant collagen, Comparative Example 7 is the same as Example 1 except that it only contains type 17 recombinant collagen, and Comparative Example 8 is the same as Example 1 except that it only contains elongated seaweed strip extract.

[0086] The compositions of the above-mentioned embodiments and comparative examples can be used in various skin care products to achieve the effect of whitening and lightening pigmentation. The skin care products include various dosage forms of toner, softener, firming lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, facial mask, pressed powder, body lotion, body cream, body oil, body milk and body essence.

[0087] The skin care product comprises 5-50% of the composition obtained in Examples 1-5 and Comparative Examples 1-8, and 50-95% of conventional excipients in the field of skin care products. Conventional excipients include surfactants, thickeners, conditioners, moisturizers, preservatives, chelating agents, fragrances, and deionized water.

[0088] The following is a detailed description of the essence, that is, the skin care products of each embodiment and comparative example are essences, and the specific formula is shown in the following table.

[0089] Table 1 Raw material composition formula of Examples 1-5 and Comparative Examples 1-8 (weight percentage %)

[0090]

[0091]

[0092] (2) According to the dosage of each component of Examples 1-5 and Comparative Examples 1-8 in Table 1, an essence was prepared by the following method:

[0093] a. Water phase: Place water in a container with a stirring device and stir, slowly disperse carbomer in the water, fully wet and mix xanthan gum, sodium hyaluronate, allantoin and glycerin, add them into the water, stir until completely dissolved, and heat to 75-80°C;

[0094] b. Oil phase; mix cetyl alcohol, cetearyl glucoside (and) cetearyl alcohol, squalane, and jojoba oil and heat to 75-80°C, stirring until completely dissolved;

[0095] c. Emulsification: Add the oil phase obtained in step b to the aqueous phase obtained in step a and homogenize at 2000-4000rpm for 4-6 minutes;

[0096] d. Add arginine, stir at 250-350 rpm for 2-4 min, and cool to 40-50°C with stirring;

[0097] e. Add (daily use) flavor, phenoxyethanol, ethylhexylglycerin, stir at 250-350rpm for 2-4min, then homogenize at 2000-4000rpm for 2-4min;

[0098] All the above steps were strictly controlled for sterility.

[0099] f. After cooling to 35-38°C, add rice fermentation liquid, type 3 recombinant collagen, type 17 recombinant collagen and Seaweed extract, stir at 250-350rpm for 4-6min, and then homogenize at 2000-4000rpm for 4-6min to obtain the essence. After passing the test, filter the material and test the bacterial content, toxins, immunogenicity, etc. for quality control.

[0100] Effect test

[0101] Change rate = (analysis value after using the product - analysis value before using the product) ÷ analysis value before using the product × 100%

[0102] 1. Spot removal test

[0103] Subjects: A total of 130 subjects, 50 males and 80 females, aged 18 to 60 years old, met the voluntary inclusion criteria and were divided into 13 groups, 10 in each group. Written informed consent was signed. Before inclusion, subjects were asked a series of questions about medical history and health status according to the inclusion and exclusion criteria. At the same time, the skin of the test site was evaluated for compliance and skin color test screening, and the results were recorded;

[0104] Environmental conditions: During the test, visual assessment and instrument testing are carried out in an environment with a temperature of 21±1℃ and a relative humidity of 50±10%RH. Visual assessment is carried out under constant lighting conditions (fluorescent lamps or LED lighting with a color temperature of 5500-6500K). The subjects need to adapt to this environmental condition for at least 30 minutes before evaluation and testing can be carried out.

[0105] Test products: cosmetics obtained from Examples 1-5 and Comparative Examples 1-8

[0106] Experimental instruments: UV solar simulator: UVSOLARSIMULATOR, MODEL601-300V2.5 (all performance indicators meet the requirements of the measurement specifications), skin colorimeter: CHROMAMETERCR-400, skin melanin detector: skin melanin and hemoglobin tester MX18, skin color card: skin color from light to dark, 0 points represents the lightest skin color, and 9 points represents the darkest skin color.

[0107] Experimental method: Qualified subjects entered the stage of establishing the human skin melanization model. First, the minimum erythema dose (MED) of each subject's test site was determined, that is, the minimum dose of ultraviolet radiation required to cause a clearly visible erythema on the skin and reach most of the irradiation point (J / m 2 ) or the shortest time (s). Then, after selecting the test area at the test site, use a UV sunlight simulator to irradiate the same irradiation point once a day at a dose of 0.75 times the MED for 4 consecutive days.

[0108] The 4 days after the irradiation is the skin darkening period, and no treatment is required;

[0109] On the fifth day after the irradiation, the skin color of each test area was visually evaluated and instrumentally tested, and the test area with poor consistency (the area where the difference between the ITA° value and the average value of all test areas was greater than 5) was eliminated. On the same day, the cosmetics obtained in Examples 1-5 and Comparative Examples 1-8 were applied to each blackening test area according to different groups. The application area was 3×3 cm, the interval between each test area was greater than 1.0 cm, and the application amount was 2.00±0.05 mg / cm 2 , the test product was applied twice a day, with an interval of no less than 4 hours between the two applications;

[0110] The test substance was applied continuously for 4 weeks, and the skin color was visually evaluated and instrumentally tested at 1 week, 2 weeks, 3 weeks, and 4 weeks after application, and the results were recorded.

[0111] Experimental result calculation method: Spot area change rate = (average spot area of ​​10 people in each group after using the test sample - average spot area of ​​10 people in each group before using the test sample, i.e., at T0) / average spot area of ​​10 people in each group before using the test sample, i.e., at T0 * 100%. See Table 2 below for details:

[0112] Table 2 Changes in mean values ​​of skin spots

[0113] After 1 week of use (%) After 2 weeks of use (%) After 4 weeks of use (%) Example 1 16.32 19.56 23.64 Example 2 20.28 23.15 28.06 Example 3 23.44 26.38 32.49 Example 4 25.55 28.98 35.07 Example 5 27.06 31.17 37.78 Comparative Example 1 11.03 12.68 14.05 Comparative Example 2 11.58 13.31 14.86 Comparative Example 3 10.96 11.65 12.68 Comparative Example 4 10.43 11.44 12.09 Comparative Example 5 8.08 9.42 10.64 Comparative Example 6 7.15 8.34 10.18 Comparative Example 7 7.22 8.16 9.58 Comparative Example 8 6.48 7.40 8.26

[0114] According to instrument measurement, 100% of the subjects had their spots improved in the 1st, 2nd and 4th week of product use.

[0115] It can be seen from Table 2 above that after the subjects used the test products in Examples 1-5 and Comparative Examples 1-8, the average values ​​of the subjects' skin spots were generally reduced compared to the initial values, among which the skin spot content of the samples using Examples 1-5 was reduced at a higher rate. Moreover, the higher the proportion of the functional component in the cosmetics, the better the effect. When only one (Comparative Examples 5-8) or three (Comparative Examples 1-4) of the four functional components were contained, the effect was obviously not as good as that of Examples 1-5. Therefore, it is proved that the four functional components of the present invention have a synergistic effect.

[0116] 2. Visia Experiment

[0117] Subjects: 104 healthy volunteers with facial spots, all female, aged 30-55, divided into 13 groups, 8 people in each group. Changes of facial spots on the subjects were detected.

[0118] All subjects were healthy volunteers who had no skin diseases or other major diseases in the past three months and had not taken any antibiotics or hormone drugs. Written informed consent was provided to all subjects before the start of the study, and the subjects signed and confirmed after sufficient communication. This study was strictly supervised and guided by the Ethics Committee throughout the study, and the personal privacy information and data security of the subjects were strictly protected.

[0119] Environmental conditions: The experimental environmental conditions (temperature 20-22°C, humidity 40-60%) were set according to the experimental requirements, and the subjects were allowed to adapt to the experimental environment for more than 15-30 minutes.

[0120] Experimental instrument: Visia skin detector

[0121] Experimental method: 104 people were divided into 13 groups, 8 people in each group, and the groups used Examples 1-5 and Comparative Examples 1-8 respectively, once a day in the morning and evening, for 28 consecutive days. The experimenters collected facial image information and analyzed photos using Visia on D0, D7, D14 and D28. The data were statistically analyzed using SPSS28.0, and the test data were tested for normal distribution. If the test data was normally distributed, the T test method was used for statistical analysis; if the test data was non-normally distributed, the rank sum test method was used for statistical analysis. The rank sum test was used for statistical analysis of graded data. The statistical method significance level p < 0.05.

[0122] Feature counting means counting feature parameters without calculating their size and strength. A smaller value indicates better skin. Score means evaluating the size, area, and strength of feature parameters. A smaller score indicates better skin. See the table below for specific results:

[0123] Table 3 Changes in mean values ​​of skin spots

[0124] After 1 week of use (%) After 2 weeks of use (%) After 4 weeks of use (%) Example 1 -13.26 -16.74 -18.66 Example 2 -14.34 -17.58 -19.69 Example 3 -16.03 -18.96 -20.87 Example 4 -17.25 -19.79 -21.47 Example 5 -18.15 -20.61 -22.06 Comparative Example 1 -11.05 -12.64 -13.44 Comparative Example 2 -10.87 -11.85 -12.96 Comparative Example 3 -10.58 -11.23 -12.84 Comparative Example 4 -11.35 -12.76 -13.90 Comparative Example 5 -5.26 -6.64 -7.34 Comparative Example 6 -4.33 -5.38 -6.01 Comparative Example 7 -4.37 -5.50 -6.26 Comparative Example 8 -3.16 -4.40 -5.77

[0125] According to instrument measurement, 100% of the subjects' spots were improved after 7th, 14th and 28th days of product use.

[0126] It can be seen from Table 3 above that after the subjects used the test products in Examples 1-5 and Comparative Examples 1-8, the average number of spots on the subjects was generally reduced compared with the other groups, among which the samples using Examples 1-5 had a more obvious skin spot removal effect, and the higher the content of the functional component (Examples 2-5), the better the effect, and when only one of the four functional components (Comparative Examples 5-8) or three (Comparative Examples 1-4) was contained, the effect was obviously not as good as that of Examples 1-5. Therefore, it is proved that the four functional components of the present invention have a synergistic effect.

[0127] Figure 1 The Visia skin spot diagram using the product obtained in Example 1, wherein the left figure is the Visia skin spot diagram before use, and the right figure is the Visia skin spot diagram after 4 weeks of use. It can be seen from the figure that the number and area of ​​spots are well improved, and the skin brightness is significantly improved, and the overall skin color is more uniform, indicating that the product of the present invention has a good whitening and lightening effect.

[0128] In summary, it can be seen that the formula of the present invention cleverly combines the four effective ingredients of rice fermentation liquid, type 3 recombinant collagen, type 17 recombinant collagen, and elongated seaweed extract, which can exert a better synergistic effect, thereby improving the whitening and pigmentation effect of the product.

[0129] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the invention patent. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the attached claims.

Claims

1. A composition having the function of brightening and lightening pigmentation, characterized in that: The composition comprises rice fermentation liquid, type 3 recombinant collagen, type 17 recombinant collagen and elongated seaweed extract in a mass ratio of (0.1-80):(0.1-50):(0.1-50):(0.1-30).

2. A composition having the function of brightening and lightening pigmentation according to claim 1, characterized in that: The mass ratio of the rice fermentation liquid, type 3 recombinant collagen, type 17 recombinant collagen and the extract of Laminaria japonica is (1-14): (1-14): (1-18): (1-15).

3. A composition having the function of brightening and lightening pigmentation according to claim 1, characterized in that: The mass ratio of the rice fermentation liquid, type 3 recombinant collagen, type 17 recombinant collagen and the seaweed extract is (1-10): (1-10): (1-10): (1-10).

4. A skin care product containing the composition according to any one of claims 1 to 3, characterized in that: The weight percentage of the composition in skin care products is 5-50%.

5. The skin care product according to claim 4, characterized in that: The weight percentage of the composition in skin care products is 10-30%.

6. The skin care product according to claim 4, characterized in that The skin care products include toner, softener, firming lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, facial mask, powder, body lotion, body cream, body oil, body milk or body essence.

7. The skin care product according to claim 5, characterized in that: The skin care product also contains 50-95% of matrix auxiliary materials.

8. A skin care product according to claim 7, characterized in that: The matrix excipients account for 70-90% of the skin care products, and the matrix excipients include one or more of surfactants, thickeners, conditioners, moisturizers, preservatives, chelating agents, flavors, and deionized water.

9. A skin care product according to claim 6, characterized in that: The skin care product is an essence, which includes the following components in percentage by weight: Phase A auxiliary materials include: water to 100; Xanthan gum 0.01~0.1; Carbomer 0.05~1; Sodium hyaluronate 0.01~0.8; Allantoin 0.1~2; Glycerol 1-10; Phase B auxiliary materials include: Jojoba oil 1-3; Cetyl alcohol 0.1~1; Cetearyl Glucoside (and) Cetearyl Alcohol 0.1~1; Squalane 0.1~2; Phase C auxiliary materials include: Arginine 0.01~2; Phase D auxiliary materials include: (Daily use) essence 0.01~1; Phenoxyethanol 0.1~0.5; Ethylhexylglycerin 0.01~0.5; Phase E materials include: Rice fermentation liquid 1-14; Type 3 recombinant collagen 1-14; Recombinant collagen types 1-17; 1-15 ml of elongated seaweed extract.

10. A skin care product according to claim 9, characterized in that: The essence is prepared by the following method: a. Water phase: Place water in a container with a stirring device and stir, slowly disperse carbomer in the water, fully wet and mix xanthan gum, sodium hyaluronate, allantoin and glycerin, add them into the water, stir until completely dissolved, and heat to 75-80°C; b. Oil phase; mix cetyl alcohol, cetearyl glucoside (and) cetearyl alcohol, squalane, and jojoba oil and heat to 75-80°C, stirring until completely dissolved; c. Emulsification: Add the oil phase obtained in step b to the aqueous phase obtained in step a and homogenize at 2000-4000rpm for 4-6 minutes; d. Add arginine, stir at 250-350 rpm for 2-4 min, and cool to 40-50°C with stirring; e. Add (daily use) flavor, phenoxyethanol, ethylhexylglycerin, stir at 250-350rpm for 2-4min, then homogenize at 2000-4000rpm for 2-4min; f. After cooling to 35-38°C, add rice fermentation liquid, type 3 recombinant collagen, type 17 recombinant collagen and seaweed extract, stir at 250-350rpm for 4-6min, and then homogenize at 2000-4000rpm for 4-6 minutes to obtain the essence.

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