Anti-acne composition and skin care product applying same

Abstract

The invention provides an anti-acne composition and a skin care product applying the anti-acne composition, and the anti-acne composition comprises the following components in parts by weight: 1-2 parts of cinnamyl aldehyde essential oil, 5-10 parts of a licorice extract, 10-20 parts of shea butter, and 5-10 parts of jojoba oil. The acne composition has the advantages of resisting bacteria, diminishing inflammation, relieving skin acne, being high in safety, promoting wound healing and inhibiting scar formation, and solves the problem of strong side effects caused by using western medicines to relieve acne in the prior art.

Description

Technical Field

[0001] The present application relates to the technical field of skin care products or pharmaceutical compositions, and in particular to an anti-acne composition and a skin care product using the same. Background Art

[0002] Acne is a common skin disease with a complex pathogenesis, which is mainly related to factors such as excessive sebum secretion, blocked hair follicles, bacterial infection and inflammatory response. Different degrees of acne will have different symptoms, resulting in different degrees of facial damage. Common manifestations are mainly acne, papules, pustules, and in severe cases, cysts, nodules and scars, often accompanied by seborrhea. Although acne does not affect normal actions, it has a great impact on the patient's psychology and social life because it often occurs on the face.

[0003] Propionibacterium acnes is a Gram-positive bacterium that often colonizes human skin and is an important component of the skin microbiome. The skin microbiome and the host together build the human skin barrier and help maintain the health of the host. For a long time, Propionibacterium acnes has been considered the microorganism most closely associated with acne, and there is a lot of evidence that it plays an important role in the pathogenesis of acne.

[0004] The current means of combating Propionibacterium acnes rely on traditional antibiotic drugs. For patients with mild and moderate acne, the first choice is still traditional antibiotic treatment. Although it reduces the number of Propionibacterium acnes to a certain extent, it also affects other skin commensal bacteria and destroys the skin barrier. In addition, antibiotics are inevitably cytotoxic, and the problem of microbial resistance caused by the abuse of antibiotics is equally serious. For patients with severe acne, isotretinoin is the most commonly used retinoid drug, but its potential risks to male reproduction are controversial, and there are also effects on human liver and kidney function and the potential risk of causing depression in patients, which greatly limits its use scenarios. Oral hormone drugs are another type of means, which can alleviate the occurrence of acne to a certain extent, but there are also risks such as allergic reactions and hormone secretion disorders.

[0005] The antibiotics and retinoic acid drugs used in the existing treatment methods have problems such as large side effects and drug resistance. Therefore, it is of great significance to develop a new, safe, natural and effective anti-acne skin care product containing Chinese herbal medicine. Summary of the invention

[0006] In order to solve the problems in the prior art, the present application provides an anti-acne composition and a skin care product using the same.

[0007] In a first aspect, the present application provides an anti-acne composition, which adopts the following technical solution:

[0008] An anti-acne composition comprises, by weight, 1-2 parts of cinnamaldehyde essential oil, 5-10 parts of liquorice extract, 10-20 parts of shea butter and 5-10 parts of jojoba oil.

[0009] Preferably, the cinnamaldehyde essential oil is prepared by distillation.

[0010] The anti-acne composition prepared by combining cinnamaldehyde essential oil, licorice extract, shea butter and jojoba oil in the present application has a good inhibitory effect on Propionibacterium acnes and inflammatory mediators / factors.

[0011] First, on the one hand, trans-cinnamaldehyde (TC) in cinnamaldehyde essential oil contains a hydrophobic six-carbon aromatic phenol group. The phenol-rich molecules can pass through the phospholipid bilayer of the cell wall of Propionibacterium acnes and bind to proteins, hindering the normal function of the cell membrane. Changes in cell membrane permeability and the loss of functional proteins that transport molecules and ions will disrupt microbial cells, subsequently leading to cytoplasm coagulation, enzyme denaturation, and loss of metabolites and ions; and trans-cinnamaldehyde (TC) can inhibit the polymerization of filamentous temperature-sensitive protein FtsZ and disrupt the Z-ring morphology formed by FtsZ polymerization, thereby achieving the purpose of inhibiting bacterial division. acnes, thereby significantly inhibiting the growth and proliferation of Propionibacterium acnes. Furthermore, trans-cinnamaldehyde (TC) can also inhibit the secretion of ATP synthase (F1F10-ATPase), thereby inhibiting the synthesis of ATP and reducing the ATP level in Propionibacterium acnes, thereby damaging the mitochondrial function of Propionibacterium acnes, thereby inducing functional abnormalities of Propionibacterium acnes and inhibiting the proliferation of Propionibacterium acnes. On the other hand, trans-cinnamaldehyde (TC) can inhibit the expression of some genes on the bacterial membrane, thereby affecting the material transport and resistance to osmotic stress of Propionibacterium acnes and reducing its structural and functional integrity.

[0012] Secondly, glycyrrhizic acid in licorice extract can cooperate with cinnamaldehyde essential oil to significantly inhibit the production of inflammatory factors. Glycyrrhizic acid can also inhibit the phosphorylation of key enzymes in the inflammatory pathway by binding to phospholipase A2 or affecting the pathway of arachidonic acid, thereby reducing the levels of inflammatory mediators such as prostaglandins and leukotrienes, thereby alleviating skin acne problems.

[0013] Finally, the linolenic acid and linoleic acid in jojoba oil and shea butter can inhibit the production and phagocytosis of reactive oxygen species in neutrophils, preventing low concentrations of linoleic acid from stimulating epithelial keratinization to form acne when passing through the sebaceous gland ducts, which in turn leads to blockage of the hair follicle ducts and hinders the outflow of sebum, thus avoiding the induction of acne; moreover, the palmitic acid in shea butter can inhibit the activity of 5-α reductase, thereby reducing the conversion of testosterone into dihydrotestosterone (DHT), thereby affecting androgen levels and avoiding hair follicle duct blockage and acne problems caused by abnormal androgen levels.

[0014] More importantly, the linolenic acid and linoleic acid in jojoba oil and shea butter can maintain the epidermal barrier function, thereby alleviating skin acne problems; this is because, when the skin barrier is damaged, water loss increases and the skin becomes dry, which stimulates the sebaceous glands to secrete more oil to compensate. Excessive oil can easily clog the pores, providing a favorable environment for the growth of Propionibacterium acnes. At the same time, the damaged skin barrier cannot effectively resist external stimuli, triggering an inflammatory response, further aggravating acne symptoms.

[0015] In summary, the mutual cooperation of the four ingredients in the anti-inflammatory and anti-acne composition of the present application can not only alleviate skin acne problems by inhibiting the proliferation of Propionibacterium acnes and destroying the integrity of its bacterial structure, but also alleviate skin acne problems by reducing sebaceous gland secretion and adjusting androgen levels, and establish a good protective barrier on the skin surface, promote the recovery of acne-prone skin, help promote the skin's absorption of effective ingredients, and form a virtuous cycle for repairing acne problems; in addition, the anti-acne composition in the present application has high safety, will not have side effects during use, and can also significantly reduce the occurrence of drug resistance.

[0016] Preferably, the mass ratio of the cinnamaldehyde essential oil to the licorice extract is 1:4-5, and the total weight parts of the cinnamaldehyde essential oil and the licorice extract is 6-10 parts.

[0017] By regulating the mass ratio of cinnamaldehyde essential oil to licorice extract to remain within the above range, the two can exert a significant synergistic effect to inhibit the proliferation of Propionibacterium acnes, thereby significantly improving skin problems caused by acne.

[0018] Preferably, the composition further comprises 2-4 parts of a skin conditioning agent, wherein the skin conditioning agent comprises ceramide and panthenol.

[0019] By introducing ceramide and panthenol as skin conditioners in the above composition, on the one hand, the combination of the two can improve the sebum membrane and inhibit excessive secretion of the sebum membrane, thereby helping to alleviate acne problems; on the other hand, the combination of the two can help the skin achieve water-oil balance, thereby effectively maintaining the hydration of the skin, and promoting the division and regeneration of skin cells, promoting the thickening of the stratum corneum, thereby enhancing the tolerance of the skin, reducing the sensitivity of acne skin, and establishing a good barrier protection effect on the skin surface, providing a stable skin foundation for the effective absorption of other ingredients in the composition, and helping to improve the improvement effect of the composition on acne problems.

[0020] Preferably, the mass ratio of the ceramide to the panthenol is 2-3:3-5.

[0021] Preferably, 1-3 parts of salicylic acid are also included.

[0022] Salicylic acid can be combined with licorice extract and cinnamaldehyde essential oil to significantly improve the anti-inflammatory effect. Salicylic acid can also reduce oil secretion on the skin surface and promote the exfoliation of the stratum corneum, thereby improving pore blockage and inflammatory response, and thus helping to alleviate skin acne problems.

[0023] In a second aspect, the present application provides a skin care product, which adopts the following technical solution:

[0024] A skin care product comprises the anti-acne composition as described above, wherein the anti-acne composition accounts for 20%-30% by weight of the skin care product.

[0025] Preferably, the anti-acne and anti-inflammatory cosmetic further comprises a moisturizer, an antioxidant, an emulsifier, a preservative, a thickener, and a pH adjuster.

[0026] Preferably, in the skin care product, by weight percentage, the anti-acne composition is 20-30%, the moisturizer is 13%-16%, the antioxidant is 6%-9%, the emulsifier is 4%-6%, the preservative is 2%-4%, the thickener is 1%-3%, and the pH adjuster is 0.3%-0.7%.

[0027] Preferably, the moisturizer includes at least one of glycerin, butylene glycol, hyaluronic acid, and polydimethylsiloxane; the antioxidant includes at least one of vegetable olive oil, vitamin C, and vitamin E; the emulsifier includes at least one of stearic acid and cetearyl alcohol, the preservative includes at least one of phenoxyethanol, carbomer, and potassium sorbate; the thickener includes xanthan gum; and the pH adjuster includes citric acid.

[0028] Preferably, the skin care product further comprises a solvent, and the solvent comprises water.

[0029] Preferably, the skin care product further comprises vanillin.

[0030] By introducing the above-mentioned moisturizing agent ingredients into skin care products, the hypoallergenic, non-irritating and non-acnegenic properties of the skin care products can be ensured, which helps to further improve the improvement effect of skin care products on acne. In addition, the nourishing ingredients in the skin care products can provide the skin with necessary nutrition and moisture, enhance the skin barrier function, and keep the skin soft and moisturized. After long-term use, users will find that the skin gradually regains a healthy luster, even skin tone, and is delicate and smooth; therefore, the skin care products of this application are not only suitable for people with acne problems, but also suitable for a wide range of consumers who pursue healthy skin quality. It is a safe, effective skin care product with a good user experience.

[0031] In a third aspect, the present application provides a method for preparing a skin care product, using the following technical solution:

[0032] A method for preparing a skin care product comprises the following steps:

[0033] S1, mixing the shea butter and the jojoba oil, heating to 70-80° C., and stirring to obtain a first mixture;

[0034] S2, adding the cinnamaldehyde essential oil, the licorice extract, and the salicylic acid to the first mixture, and continuing stirring at 60-70° C. to obtain a second mixture;

[0035] S3, mixing the ceramide and the panthenol evenly and then mixing with the second mixture, stirring for 15-20 minutes to obtain a third mixture;

[0036] S4, adding the moisturizer, the antioxidant, the emulsifier, the preservative, the thickener, and the pH adjuster to the third mixture, and continuously stirring to obtain an emulsion to be treated;

[0037] S5, lowering the temperature of the emulsion to be treated to 40-50°C, stirring for 30-40 minutes, and filtering to obtain the skin care product. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 This is the gas chromatography-mass spectrometry analysis diagram of cinnamaldehyde essential oil in this application.

[0039] Figure 2 This is a graph showing the effect of an acute skin irritation test of the anti-acne skin care product of Example 1 of the present application for 72 hours (Figures a and b represent rabbits No. 1 to 4 used in Example 1).

[0040] Figure 3 These are the effect diagrams of multiple skin irritation tests of the anti-acne skin care product 14d (Figures a and b represent rabbits No. 5 to 8 used in Example 1) of Example 1 of the present application.

[0041] Figure 4 These are pictures of the facial condition of the subjects before (a) and after (b) using the anti-acne skin care product of Example 1 of the present application. DETAILED DESCRIPTION

[0042] For better understanding and implementation, the technical solution of the present application will be clearly and completely described below in conjunction with embodiments. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all of the embodiments.

[0043] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used herein in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application.

[0044] Unless otherwise indicated, all numerical values ​​expressing quantities of ingredients, reaction conditions, and so forth used in the specification and claims are to be understood as modified by the term “about.” Accordingly, unless indicated to the contrary, the numerical parameters set forth herein are approximations that can vary depending upon the desired properties desired to be obtained.

[0045] As used herein, "and / or" means one or all of the mentioned elements.

[0046] As used herein, “including” and “comprising” encompasses the case where only the stated elements are present as well as the case where there are other elements other than the stated elements.

[0047] All percentages in this application are by weight unless otherwise stated.

[0048] Unless otherwise indicated, "a", "an", "an" and "the" used in this specification are intended to include "at least one" or "one or more". For example, "a component" means one or more components, so more than one component may be considered and may be employed or used in the implementation of the embodiment.

[0049] Example 1

[0050] 1. Preparation of anti-acne composition

[0051] The anti-acne composition comprises 1.5 parts of cinnamaldehyde essential oil, 8 parts of licorice extract, 15.5 parts of shea butter, and 8 parts of jojoba oil;

[0052] 2. Preparation of anti-acne skin care products

[0053] Calculated by weight percentage, the anti-acne skin care product includes 21% anti-acne composition, 15% moisturizer (glycerin, butylene glycol, hyaluronic acid, polydimethylsiloxane), 8% antioxidant (vegetable olive oil, vitamin C, vitamin E), 5% emulsifier (stearic acid, cetearyl alcohol), 3% preservative (phenoxyethanol, carbomer, potassium sorbate), 3% thickener (xanthan gum), 0.5% pH adjuster (citric acid), and the rest is water.

[0054] The anti-acne skin care product is prepared by a method comprising the following steps:

[0055] S1, mixing shea butter and jojoba oil, heating to 75° C., and stirring to obtain a first mixture;

[0056] S2, adding cinnamaldehyde essential oil, licorice extract and salicylic acid to the first mixture, and continuing stirring at 65° C. to obtain a second mixture;

[0057] S3, mixing ceramide and panthenol evenly, and then mixing with the second mixture, and stirring for 18 minutes to obtain a third mixture;

[0058] S4, adding a moisturizer, an antioxidant, an emulsifier, a preservative, a thickener, and a pH adjuster to the third mixture, and continuously stirring to obtain an emulsion to be treated;

[0059] S5, lowering the temperature of the emulsion to be treated to 45°C, stirring for 35 minutes, and filtering to obtain a skin care product.

[0060] The gas chromatography mass spectrometry analysis of cinnamaldehyde essential oil in this application is shown in FIG. Figure 1 shown.

[0061] The skin care product in this example was used to conduct a 72-hour acute skin irritation test on the back skin of rabbits No. 1-4. The test results are shown in the figure below: Figure 2 shown.

[0062] The skin care product in this example was used to conduct multiple skin irritation tests on the back skin of rabbits No. 5-8 for 14 days. The test results are shown in the figure below: Figure 3 shown.

[0063] The facial states of the subjects before and after using the skin care product of this embodiment are shown in the figure below. Figure 4 shown.

[0064] Example 2

[0065] 1. Preparation of anti-acne composition

[0066] The anti-acne composition comprises 2 parts of cinnamaldehyde essential oil, 5 parts of licorice extract, 19 parts of shea butter and 10 parts of jojoba oil;

[0067] 2. Preparation of anti-acne skin care products

[0068] Calculated by weight percentage, the anti-acne skin care product includes 20% anti-acne composition, 13% moisturizer (glycerin, butylene glycol, hyaluronic acid, polydimethylsiloxane), 6% antioxidant (vegetable olive oil, vitamin C, vitamin E), 4% emulsifier (stearic acid, cetearyl alcohol), 2% preservative (phenoxyethanol, carbomer, potassium sorbate), 1% thickener (xanthan gum), 0.3% pH adjuster (citric acid), and the rest is water.

[0069] The anti-acne skin care product is prepared by a method comprising the following steps:

[0070] S1, mixing shea butter and jojoba oil, heating to 80° C., and stirring to obtain a first mixture;

[0071] S2, adding cinnamaldehyde essential oil, licorice extract and salicylic acid to the first mixture, and continuing stirring at 60° C. to obtain a second mixture;

[0072] S3, mixing ceramide and panthenol evenly, and then mixing with the second mixture, and stirring for 15 minutes to obtain a third mixture;

[0073] S4, adding a moisturizer, an antioxidant, an emulsifier, a preservative, a thickener, and a pH adjuster to the third mixture, and continuously stirring to obtain an emulsion to be treated;

[0074] S5, lowering the temperature of the emulsion to be treated to 50° C., stirring for 30 minutes, and filtering to obtain a skin care product.

[0075] Example 3

[0076] 1. Preparation of anti-acne composition

[0077] The anti-acne composition comprises 1 part of cinnamaldehyde essential oil, 5 parts of licorice extract, 10 parts of shea butter and 5 parts of jojoba oil;

[0078] 2. Preparation of anti-acne skin care products

[0079] Calculated by weight percentage, the anti-acne skin care product includes 30% of the anti-acne composition, 16% of the moisturizer (glycerin, butylene glycol, hyaluronic acid, polydimethylsiloxane), 9% of the antioxidant (vegetable olive oil, vitamin C, vitamin E), 6% of the emulsifier (stearic acid, cetearyl alcohol), 4% of the preservative (phenoxyethanol, carbomer, potassium sorbate), 3% of the thickener (xanthan gum), 0.7% of the pH adjuster (citric acid), and the rest is water.

[0080] The anti-acne skin care product is prepared by a method comprising the following steps:

[0081] S1, mixing shea butter and jojoba oil, heating to 70° C., and stirring to obtain a first mixture;

[0082] S2, adding cinnamaldehyde essential oil, licorice extract and salicylic acid to the first mixture, and continuing stirring at 70° C. to obtain a second mixture;

[0083] S3, mixing ceramide and panthenol evenly, and then mixing with the second mixture, and stirring for 20 minutes to obtain a third mixture;

[0084] S4, adding a moisturizer, an antioxidant, an emulsifier, a preservative, a thickener, and a pH adjuster to the third mixture, and continuously stirring to obtain an emulsion to be treated;

[0085] S5, lowering the temperature of the emulsion to be treated to 40°C, stirring for 40 minutes, and filtering to obtain a skin care product.

[0086] Example 4

[0087] The difference between this embodiment and embodiment 1 is that the mass ratio of cinnamaldehyde essential oil to licorice extract in the anti-acne composition is 1:4, and the total weight parts of cinnamaldehyde essential oil and licorice extract is 9.5 parts; other steps and parameter settings are consistent with embodiment 1.

[0088] Example 5

[0089] The difference between this embodiment and embodiment 1 is that the mass ratio of cinnamaldehyde essential oil to licorice extract in the anti-acne composition is 1:5, and the total weight parts of cinnamaldehyde essential oil and licorice extract is 9.5 parts; other steps and parameter settings are consistent with embodiment 1.

[0090] Example 6

[0091] The difference between this embodiment and embodiment 4 is that the anti-acne extract further includes 2 parts of skin conditioner, and the skin conditioner includes ceramide and panthenol in a mass ratio of 2:3; other steps and parameter settings are consistent with embodiment 1.

[0092] Example 7

[0093] The difference between this embodiment and embodiment 4 is that the anti-acne extract further includes 4 parts of skin conditioners, and the skin conditioners include ceramide and panthenol in a mass ratio of 3:5; other steps and parameter settings are consistent with embodiment 1.

[0094] Example 8

[0095] The difference between this embodiment and embodiment 7 is that the anti-acne extract further includes 2 parts of salicylic acid; the other steps and parameter settings are consistent with those of embodiment 1.

[0096] Example 9

[0097] The difference between this embodiment and embodiment 1 is that the mass proportion of the anti-acne extract used in the anti-acne skin care product is 10%; the other steps and parameter settings are consistent with those in embodiment 1.

[0098] Example 10

[0099] The difference between this embodiment and embodiment 1 is that the mass proportion of the anti-acne extract used in the anti-acne skin care product is 38%; the other steps and parameter settings are consistent with those in embodiment 1.

[0100] Comparative Example 1

[0101] The difference between this comparative example and Example 1 is that the anti-acne composition does not contain cinnamaldehyde essential oil; other steps and parameter settings are consistent with Example 1.

[0102] Comparative Example 2

[0103] The difference between this comparative example and Example 1 is that the anti-acne composition does not contain licorice extract; other steps and parameter settings are consistent with Example 1.

[0104] Comparative Example 3

[0105] The difference between this comparative example and Example 1 is that the anti-acne composition does not contain shea butter and jojoba oil; other steps and parameter settings are consistent with Example 1.

[0106] Comparative Example 4

[0107] The difference between this comparative example and Example 1 is that eucalyptus oil in equal parts by weight is used instead of cinnamaldehyde essential oil; other steps and parameter settings are consistent with those in Example 1.

[0108] Comparative Example 5

[0109] The difference between this comparative example and Example 1 is that galangal oil in equal parts by weight is used instead of cinnamaldehyde essential oil; other steps and parameter settings are consistent with those in Example 1.

[0110] Comparative Example 6

[0111] The difference between this comparative example and Example 1 is that tea tree oil in equal parts by weight is used instead of cinnamaldehyde essential oil; other steps and parameter settings are consistent with Example 1.

[0112] Test Method

[0113] 1. Gas chromatography-mass spectrometry analysis

[0114] The cinnamaldehyde essential oil in this application was subjected to gas chromatography-mass spectrometry analysis, and chemical analysis was performed using a gas chromatograph (GC) (Hewlett Packard, Hewlett Manufacturing Company, USA) equipped with an HP-5MS chromatographic column (30m×0.25mm×0.25μm). The oven temperature was set to 40°C for 1 min, then raised to 250°C at a rate of 6°C / min and maintained at this temperature for 4 min. Helium was used as the carrier gas at a rate of 1.0 mL / min, and the scanning range was 40-500 amu.

[0115] 2. Irritation Test

[0116] The anti-acne skin care products prepared in the above examples and comparative examples were subjected to acute skin irritation tests and multiple skin irritation tests.

[0117] 2.1 Acute skin irritation test

[0118] The test steps are as follows:

[0119] Before the test, the hair on the back of the rabbits was trimmed to ensure that the anti-acne skin care products could directly contact the skin. 0.5 mL of the anti-acne skin care products (prepared with 40% ethanol) and 40% ethanol solution (control group) were applied to the back of the rabbits, respectively, with the application area of ​​2.5 cm × 2.5 cm. The drug area was fixed with cellophane, gauze and bandages for 4 hours. After 4 hours, the dressings were removed and the reaction of the skin in the test area was observed.

[0120] The observation time point was 72 hours after the removal of the dressing, and the skin reaction was recorded and scored; the scoring criteria included redness, swelling, peeling, ulceration and other manifestations, and the average score at each time point was calculated.

[0121] Scoring criteria: No irritation is 0, increasing in degree, with severe irritation being 9-10.

[0122] The skin irritation intensity was determined based on the highest integrated mean value at each time point, such as mild, moderate or severe irritation, and the test data were recorded in Table 1.

[0123] Table 1

[0124]

[0125]

[0126] 2.2 Multiple skin irritation tests

[0127] Test steps: Before the test, the hair on the back of the rabbits was trimmed to ensure that the anti-acne skin care products could directly contact the skin. 0.5 mL of the anti-acne skin care products and 40% ethanol solution (control group) were applied on the back of the rabbits, and the application area was 25 cm × 2.5 cm.

[0128] Apply once a day for 14 consecutive days. Starting from the second day, trim the hair before each application and remove the residual drug; after each removal of the drug, record and score the skin reaction and calculate the daily average score;

[0129] Scoring criteria: No irritation is 0, increasing in degree, with severe irritation being 9-10.

[0130] Based on the daily scores, the rated skin irritation intensity is recorded in Table 2.

[0131] Table 2

[0132]

[0133]

[0134] During the above irritation test, the temperature range of the animal breeding area was 22-26℃, and the temperature range was 40-70%, which met the test requirements. The weight of the domestic rabbits was 2.1kg-2.4kg, which met the test requirements. The domestic rabbits grew normally after contacting the anti-acne skin care products, and there were no abnormalities. During the test and the end of the test, the animal mortality rate was 0%.

[0135] 3. Antibacterial performance test

[0136] The antibacterial performance of the skin care products prepared in the above examples and comparative examples was tested, and the specific test steps are as follows:

[0137] P. acnes DMST 14916 was plated on BHI agar and stored in an anaerobic jar at 37°C for 48 h. The inoculated broth was adjusted to a 0.5 McFarland standard turbidity and an optical density (OD) of 0.08-0.13 at a wavelength of 600 nm.

[0138] Agar disk diffusion test: 300 μL of P. acnes suspension (1.5×108 CFU / mL) was spread on BHI agar. A paper disk (6.0 mm in diameter) was impregnated with 20 μL of anti-acne skin care product, and then the disk was placed on the BHI agar and gently pressed. Clindamycin (1% w / v) was used as a positive control, and distilled water was used as a negative control. The inoculated plates were cultured at 37°C under an anaerobic system for 48 h. After the incubation period, the clear zone diameter was measured with a vernier caliper, and the test data were recorded in Table 3. To determine the antibacterial activity of the masking agent against P. acnes, the masking agent (0.5 g) was spread in the agar wells instead of on the paper disc.

[0139] Broth dilution method: Different concentrations (0.25% w / v, 0.5% w / v, 1.0% w / v, 2.0% w / v) of essential oils were prepared by dilution with BHI broth using 2% w / v dimethyl sulfoxide (DMSO) and 2% w / v Tween-80 as solubilizers. 300 μL of P. acnes suspension (1.5×108 CFU / mL) was mixed with 2 mL of the test oil. Liquid paraffin was added and each test tube was sealed with plastic wrap and then cultured in an anaerobic jar at 37°C for 48 h. The minimum inhibitory concentration (MIC) value was taken as the lowest test sample concentration that could inhibit bacterial growth, at which a transparent mixture was observed. Clindamycin (1% w / v) was used as a positive control, while BHI broth containing a solubilizer was used as a negative control. The OD value of the test mixture was measured at a wavelength of 600 nm using a microplate reader to confirm the results. The OD values ​​were read immediately after mixing (0 h) and after 48 h of incubation, and the percentage of growth inhibition was calculated as follows:

[0140]

[0141] The test group: oil in BHI broth containing Propionibacterium acnes; the blank group: oil in BHI broth; the control group: BHI broth containing Propionibacterium acnes. The test data are recorded in Table 3.

[0142] Table 3

[0143] Serial number Transparent area diameter / mm Growth inhibition rate / % Example 1 27.00±0.01 41.54 Example 2 34.75±1.06 52.85 Example 3 31.25±1.05 48.69 Example 4 36.75±1.06 56.54 Example 5 37.75±1.05 58.08 Example 6 44.23±1.10 68.05 Example 7 45.22±1.10 69.57 Example 8 48.56±0.95 74.71 Example 9 20.65±0.55 32.08 Example 10 44.35±1.05 68.23 Comparative Example 1 21.85±1.05 33.62 Comparative Example 2 26.05±0.50 40.08 Comparative Example 3 22.05±0.55 33.92 Comparative Example 4 48.00±0.02 74.62 Comparative Example 5 44.50±0.71 68.00 Comparative Example 6 42.67±0.58 62.57

[0144] 4. Skin test

[0145] The anti-acne skin care product prepared in the above example was subjected to skin testing; 5 volunteers aged between 18 and 25 with facial acne were recruited as subjects.

[0146] 3.1 Transdermal water loss test

[0147] The skin of the above subjects was tested for transdermal water loss rate. The test method is as follows: Use the installation The transepidermal water loss rate was measured by a non-invasive skin detector with a TM300 probe (Courage & Khazak, Germany); using the open cavity method, the shorter side of the probe was gently and vertically attached to the skin surface of the test point, and the red button on the handle was pressed to start the measurement. The display screen showed the value every second. The system automatically stopped after 30 seconds, and the screen displayed the measurement results. The test results were recorded in Table 4.

[0148] 3.2 Skin stratum corneum moisture content test

[0149] The stratum corneum moisture content of the above subjects' skin was tested as follows: using the installation The non-invasive skin detector with CM825 probe (Courage & Khazak, Germany) measured the water content of the stratum corneum. The test probe was placed vertically on the skin surface at the test point, and the probe was lightly pressed. After the probe rebounded, the screen displayed the measurement result. The test was repeated three times for each part, and the average value was taken; the test results were recorded in Table 4.

[0150] Table 4

[0151] Serial number <![CDATA[Transdermal water loss rate / (g·hm -2 )]]> Water content of skin stratum corneum / (au) Example 1 12.78±5.92 30.70±11.76 Example 2 13.64±5.98 29.38±13.02 Example 3 14.26±5.86 27.52±14.29 Example 4 13.92±5.74 27.60±12.97 Example 5 12.86±5.97 26.80±12.63 Example 6 11.65±4.64 31.32±13.41 Example 7 10.45±5.08 32.76±12.57 Example 8 10.05±5.62 32.55±12.74 Example 9 11.42±6.23 22.32±12.75 Example 10 14.31±5.53 19.23±11.39 Comparative Example 1 14.59±5.35 19.40±12.81 Comparative Example 2 14.93±6.25 19.90±13.51 Comparative Example 3 16.35±4.92 17.09±13.91 Comparative Example 4 16.65±7.30 26.04±13.68 Comparative Example 5 13.34±6.78 24.73±12.74 Comparative Example 6 14.95±7.09 26.52±14.15

[0152] 5. Stability test

[0153] In order to explore the stability of this anti-acne skin care product and ensure its effective function, the light and temperature storage conditions were tested to determine its appropriate storage conditions.

[0154] The formulation of Example 8 was stored in a heating / cooling cycle from 4°C to 45°C for 6 cycles. After storage, the physical changes, including color, odor, separation, spreadability and covering properties, were determined by visual observation, and the rheometer ( The viscosity was measured using a 3700MW tumble dryer (Model DV-III, BrookfieldAMETEK, USA); and the test results are recorded in Table 5.

[0155] Table 5

[0156]

[0157]

[0158] In combination with Examples 1-3, Comparative Examples 1-7 and Tables 1-5, it can be seen that the present application prepares an anti-acne composition by combining cinnamaldehyde essential oil, licorice extract, shea butter and jojoba oil. On the one hand, it can not only inhibit the proliferation of Propionibacterium acnes by destroying the cell function of Propionibacterium acnes, but also inhibit the negative impact of Propionibacterium acnes on the skin by destroying its bacterial structure. On the other hand, it can alleviate skin acne problems by reducing androgen levels; more importantly, it can establish a good skin protection barrier on the surface of acne problem skin, thereby improving the ability of acne skin to resist external stimuli and helping to further alleviate acne problems; therefore, the anti-acne composition of the present application can significantly inhibit Propionibacterium acnes and establish an epidermal barrier effect on the skin surface after being used in skin care products, thereby effectively alleviating skin acne problems; and the skin care products of the present application have excellent storage stability.

[0159] Although the antibacterial effect of the skin care products in Comparative Examples 4-6 is improved compared with Example 1 after using eucalyptus oil, galangal oil, and tea tree oil instead of cinnamaldehyde essential oil, the irritation of the skin care products is also greatly increased and the barrier protection effect on the skin is reduced. It is not recommended to replace cinnamaldehyde essential oil as an ingredient in the anti-acne composition to alleviate skin acne problems.

[0160] In combination with Examples 1, 4-5 and Tables 1-4, it can be seen that by adjusting the mass ratio of cinnamaldehyde and licorice extract in the composition used in the skin care product, the antibacterial performance and the barrier protection function of the skin of the skin care product are significantly reduced, which helps to improve the anti-acne effect of the skin care product.

[0161] In combination with Examples 4, 6-7 and Tables 1-4, it can be seen that by introducing a skin conditioner into the anti-acne composition and controlling the mass ratio of ceramide panthenol in the skin conditioner, the antibacterial performance of the skin care product is improved and the barrier protection effect on the skin is improved. This is because, by introducing ceramide and panthenol into the anti-acne composition, on the one hand, the combination of the two can improve the sebum membrane and inhibit excessive secretion of the sebum membrane, thereby helping to alleviate the acne problem; on the other hand, the combination of the two can help the skin achieve water-oil balance, thereby effectively maintaining the hydration of the skin, and promoting the division and regeneration of skin cells, promoting the thickening of the stratum corneum, thereby enhancing the tolerance of the skin, reducing the sensitivity of acne skin, so as to establish a good barrier protection effect on the skin surface, and provide a stable skin basis for the effective absorption of other ingredients in the composition, which helps to improve the improvement effect of the composition on acne problems.

[0162] In combination with Examples 4, 7, 8 and Tables 1-4, it can be seen that the addition of salicylic acid and skin conditioners improves the irritation problem of skin care products. Salicylic acid can be combined with licorice extract and cinnamaldehyde essential oil to significantly improve the anti-inflammatory effect. Salicylic acid can also reduce oil secretion on the skin surface and promote the shedding of the stratum corneum, thereby improving pore blockage and inflammatory response, thereby helping to alleviate skin acne problems.

[0163] In combination with Examples 1, 9-10 and Tables 1-4, it can be seen that when the content of the anti-acne composition in the skin care product is too high or too low, it is not conducive to the skin care product to exert a good anti-acne effect; when the mass proportion of the anti-acne composition in the skin care product is 21%, it is the most preferred addition amount to exert the effect.

[0164] The above embodiments are only used to illustrate the technical solution of the present application rather than to limit the protection scope of the present application. Although the present application is described in detail with reference to the above embodiments, ordinary technicians in the relevant field should understand that the technical solution of the present application can be modified or equivalently replaced, but these modifications or replacements are all within the protection scope of the present application.

Claims

1. An anti-acne composition, characterized in that: Calculated by weight, the invention comprises 1-2 parts of cinnamaldehyde essential oil, 5-10 parts of licorice extract, 10-20 parts of shea butter and 5-10 parts of jojoba oil.

2. The anti-acne composition according to claim 1, characterized in that: The mass ratio of the cinnamaldehyde essential oil to the licorice extract is 1:4-5, and the total weight parts of the cinnamaldehyde essential oil and the licorice extract is 6-10 parts.

3. The anti-acne composition according to claim 1, characterized in that: The invention also comprises 2-4 parts of skin conditioning agents, wherein the skin conditioning agents include ceramide and panthenol.

4. The anti-acne composition according to claim 3, characterized in that: The mass ratio of the ceramide to the panthenol is 2-3:3-5.

5. The anti-acne composition according to claim 1, characterized in that: Also includes 1-3 parts of salicylic acid.

6. A skin care product, characterized in that: The anti-acne composition comprises the anti-acne composition according to any one of claims 1 to 5, wherein the mass proportion of the anti-acne composition in the skin care product is 20% to 30%.

7. The skin care product according to claim 6, characterized in that: The anti-acne and anti-inflammatory cosmetics also include moisturizers, antioxidants, emulsifiers, preservatives, thickeners, and pH regulators.

8. The skin care product according to claim 7, characterized in that: In the skin care product, by weight percentage, the anti-acne composition is 20-30%, the moisturizer is 13%-16%, the antioxidant is 6%-9%, the emulsifier is 4%-6%, the preservative is 2%-4%, the thickener is 1%-3%, and the pH adjuster is 0.3%-0.7%.

9. The skin care product according to claim 7, characterized in that: The moisturizer includes at least one of glycerin, butylene glycol, hyaluronic acid, and polydimethylsiloxane; the antioxidant includes at least one of vegetable olive oil, vitamin C, and vitamin E; the emulsifier includes at least one of stearic acid and cetearyl alcohol; the preservative includes at least one of phenoxyethanol, carbomer, and potassium sorbate; the thickener includes xanthan gum; and the pH adjuster includes citric acid.

10. The method for preparing the skin care product according to any one of claims 1 to 9, characterized in that: The steps include: S1, mixing the shea butter and the jojoba oil, heating to 70-80° C., and stirring to obtain a first mixture; S2, adding the cinnamaldehyde essential oil, the licorice extract, and the salicylic acid to the first mixture, and continuing stirring at 60-70° C. to obtain a second mixture; S3, mixing the ceramide and the panthenol evenly and then mixing with the second mixture, stirring for 15-20 minutes to obtain a third mixture; S4, adding the moisturizer, the antioxidant, the emulsifier, the preservative, the thickener, and the pH adjuster to the third mixture, and continuously stirring to obtain an emulsion to be treated; S5, lowering the temperature of the emulsion to be treated to 40-50°C, stirring for 30-40 minutes, and filtering to obtain the skin care product.

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